Journal of nematology最新文献

Evaluating Vacuum and Steam Heat to Eliminate Pinewood Nematodes in Naturally Infested Whole Pine Logs. 评估用真空和蒸汽加热消除受自然侵染的整根松树原木中的松材线虫。

IF 1.4 4区生物学

Journal of nematology Pub Date : 2024-10-05 eCollection Date: 2024-03-01 DOI: 10.2478/jofnem-2024-0038

J D Eisenback, Z Chen, M White

{"title":"Evaluating Vacuum and Steam Heat to Eliminate Pinewood Nematodes in Naturally Infested Whole Pine Logs.","authors":"J D Eisenback, Z Chen, M White","doi":"10.2478/jofnem-2024-0038","DOIUrl":"10.2478/jofnem-2024-0038","url":null,"abstract":"Pinewood nematodes threaten forest health and continue to interfere with international trade because they can be spread around the globe via nematode-infested wood. International Standards for Phytosanitary Measure (ISPM-15) requires that all pine wood be treated at 56°C for 30 min to ensure that all pests and pathogens are killed within sawn wood, whereas fumigation with methyl bromide is the currently approved practice and widely used in treating whole logs. A method of treatment that uses less energy and time or does not rely on environmentally damaging gases is urgently needed. Because vacuum with steam has shown promise in treating several different commodities, the purpose of this study was to use it to eradicate pinewood nematodes in whole logs. Three protocols were applied: 1) 48°C for 15 min., 2) 56°C for 30 min., and 3) 60°C for 60 min. The third protocol reduced the population to statistically zero; however, some samples contained at least 1 survivor. Unfortunately, these surviving nematodes increased in number one month after treatment, and one year later, they continued to reproduce in the wood. Therefore, this protocol needs to be further refined to remove pinewood nematodes completely. Explanation of the survival of individual nematodes within whole logs remains a matter for conjecture: 1) certain portions of the wood were somehow insulated from the heat and did not achieve the lethal temperature, and 2) survival stages may be able to survive temperatures that are deadly to the normal life stages.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11456328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of two new Pristionchus species from South Korea. 描述来自韩国的两个 Pristionchus 新种。

IF 1.4 4区生物学

Journal of nematology Pub Date : 2024-10-04 eCollection Date: 2024-03-01 DOI: 10.2478/jofnem-2024-0032

Matthias Herrmann, Natsumi Kanzaki, Christian Weiler, Penghieng Theam, Christian Rödelsperger, Ralf Sommer

引用次数: 0

Description and Molecular Characterization of a New Dorylaimid Nematode, Mesodorylaimus pini n. sp. (Nematoda: Dorylaimidae) from Korea. 来自韩国的一种新的Dorylaimid线虫Mesodorylaimus pini n. sp.（线虫纲：Dorylaimidae）的描述和分子特征。

IF 1.4 4区生物学

Journal of nematology Pub Date : 2024-09-30 eCollection Date: 2024-03-01 DOI: 10.2478/jofnem-2024-0028

Abraham Okki Mwamula, Sang Myeong Lee, Young Hak Jung, Yi Seul Kim, Dong Woon Lee

{"title":"Description and Molecular Characterization of a New Dorylaimid Nematode, Mesodorylaimus pini n. sp. (Nematoda: Dorylaimidae) from Korea.","authors":"Abraham Okki Mwamula, Sang Myeong Lee, Young Hak Jung, Yi Seul Kim, Dong Woon Lee","doi":"10.2478/jofnem-2024-0028","DOIUrl":"10.2478/jofnem-2024-0028","url":null,"abstract":"Mesodorylaimus pini n. sp., a new species isolated from the bark and cambium layer of a dead black pine tree is characterized herein using integrative taxonomy, considering both morphological and molecular phylogenetic analyses of the 18S- and 28S-rRNA genes. Mesodorylaimus pini n. sp. is characterized by having a medium-sized body 1.50-1.89 mm long; lip region angular and offset by a depression; a relatively long odontostyle (17.0-19.0 μm); vulval opening a transverse slit, positioned slightly posteriorly; pars refringens vaginae with two elongated drop-shaped to spindle-shaped sclerotizations; an intestine-prerectum junction with a long anteriorly directed conical or tongue-like projection; a relatively long female tail (115-187 μm); spicules 48.0-57.0 μm long; and regularly spaced 7-8 ventromedian supplements. It is closest to M. subtilis, especially in having similar body length and number of ventromedian supplements but can be differentiated from M. subtilis by the longer odontostyle, tongue-like projection, and longer spicules. The phylogenies based on the 18S- and 28S-rRNA sequences showed a well-supported sister relation of M. pini n. sp. with M. subtilis, M. japonicus, M. bastiani, M. pseudobastiani, Calcaridorylaimus castaneae, C. heynsi, and other member species of the group.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nematode genome announcement: A chromosome-scale genome assembly for the Pristionchus pacificus reference mapping strain PS1843. 线虫基因组公告：Pristionchus pacificus 参考制图菌株 PS1843 的染色体级基因组组装。

IF 1.4 4区生物学

Journal of nematology Pub Date : 2024-09-11 eCollection Date: 2024-03-01 DOI: 10.2478/jofnem-2024-0063

Waltraud Röseler, Ralf J Sommer, Christian Rödelsperger

{"title":"Nematode genome announcement: A chromosome-scale genome assembly for the Pristionchus pacificus reference mapping strain PS1843.","authors":"Waltraud Röseler, Ralf J Sommer, Christian Rödelsperger","doi":"10.2478/jofnem-2024-0063","DOIUrl":"10.2478/jofnem-2024-0063","url":null,"abstract":"Pristionchus pacificus is a free-living nematode that shares many features with Caenorhabditis elegans, such as its short generation time and hermaphroditism, but also exhibits novel traits, i.e., a mouth-form dimorphism that enables predation. The availability of various genetic tools and genomic resources make it a powerful model organism for comparative studies. Here, we present an updated genome of the P. pacificus strain PS1843 (Washington) that is most widely used for genetic analysis. Assembly of PacBio reads together with reference-guided scaffolding resulted in a chromosome-scale genome spanning 171Mb for the PS1843 strain. Whole genome alignments between the P. pacificus PS1843 genome and the genome of the P. pacificus reference strain PS312 (California) revealed megabase-sized regions on chromosomes III, IV, and X that explain the majority of genome size difference between both strains. The improved PS1843 genome will be useful for future forward genetic studies and evolutionary genomic comparisons at the intra-species level.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11406906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

JON 63rd Meeting 2024 Park City ABSTRACTS. JON 第 63 次会议 2024 年帕克城 ABSTRACTS.

IF 1.4 4区生物学

Journal of nematology Pub Date : 2024-09-11 eCollection Date: 2024-03-01 DOI: 10.2478/jofnem-2024-0036

引用次数: 0

Genome Announcement: Draft Genome Assembly of Heterodera humuli Generated Using Long-Read Sequencing. 基因组公告：利用长读测序生成的 Heterodera humuli 基因组组装草案。

IF 1.4 4区生物学

Journal of nematology Pub Date : 2024-08-28 eCollection Date: 2024-03-01 DOI: 10.2478/jofnem-2024-0029

Lester A Núñez-Rodríguez, Catherine L Wram, Cedar Hesse, Inga A Zasada

引用次数: 0

Taxonomy of Tobrilidae species from the Alkaline Lakes of the western Nebraska Sandhills. 内布拉斯加州西部沙丘碱性湖泊中 Tobrilidae 种类的分类。

IF 1.4 4区生物学

Journal of nematology Pub Date : 2024-08-24 eCollection Date: 2024-03-01 DOI: 10.2478/jofnem-2024-0025

Peter G Mullin, Timothy Harris, Rebecca Higgins, Enakshy Dutta, Dorota L Porazinska, Kirsten Powers, Thomas Powers

{"title":"Taxonomy of Tobrilidae species from the Alkaline Lakes of the western Nebraska Sandhills.","authors":"Peter G Mullin, Timothy Harris, Rebecca Higgins, Enakshy Dutta, Dorota L Porazinska, Kirsten Powers, Thomas Powers","doi":"10.2478/jofnem-2024-0025","DOIUrl":"10.2478/jofnem-2024-0025","url":null,"abstract":"Six distinct COI mitochondrial Haplotype Groups (HG) are morphologically, ecologically, and genetically characterized from the aquatic nematode family Tobrilidae. Collection locations included the extreme habitats of the Alkaline Lakes in the western Nebraska Sandhills and the contaminated stream, Johnson Creek, bordering the AltEn 2021 catastrophic pesticide release near the village of Mead in eastern Nebraska. Maximum likelihood and genetic distance metrics supported the genetic integrity of the haplotype groups. Discriminant function analysis of COI haplotype group datasets of combined morphological characters and soil chemistry attributes for both male and female Tobrilidae were classified correctly in all but one case. Scanning electron microscopy revealed new details about amphid apertures, male supplements, and spicules. Partial 18S gene phylogeny suggests that the genus Semitobrilus may not be a member of the subfamily Neotobrilinae, and three specimens in the 226 tobrilid dataset provide evidence of incongruence between COI and 18S derived phylogenies. Given the strong signal provided by the environmental chemistry data, tobrilid mitochondrial haplotypes may well have value as environmental indicators.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of nematicides for Meloidogyne enterolobii management in sweetpotato. 评估杀线虫剂在甘薯中的应用。

IF 1.4 4区生物学

Journal of nematology Pub Date : 2024-08-24 eCollection Date: 2024-03-01 DOI: 10.2478/jofnem-2024-0033

Jingwei Chen, Jukui Ma, Fangyuan Gao, Wei Tang, Dongjing Yang, Chengling Zhang, Zhao Liang, Yiping Xie, Houjun Sun

{"title":"Evaluation of nematicides for Meloidogyne enterolobii management in sweetpotato.","authors":"Jingwei Chen, Jukui Ma, Fangyuan Gao, Wei Tang, Dongjing Yang, Chengling Zhang, Zhao Liang, Yiping Xie, Houjun Sun","doi":"10.2478/jofnem-2024-0033","DOIUrl":"10.2478/jofnem-2024-0033","url":null,"abstract":"Sweetpotato is an important crop whose roots are consumed by people worldwide. Meloidogyne enterolobii stands out as a highly deleterious variant among the species of root-knot nematode that causes significant damage in sweetpotato. In the present study, the activity of four nematicides against M. enterolobii was assessed both in vitro and in growth cabinet experiments. After 48 hours of exposure, fluopyram and cyclobutrifluram had a greater negative effect on the motility of M. enterolobii second-stage juveniles (J2s) compared to fluensulfone and hymexazol, with respective median effective concentration (EC50) values of 0.204, 0.423, 22.335 and 216.622 mg L-1. When M. enterolobii eggs were incubated for 72 hours at the highest concentration of each nematicides, the inhibitory hatching effect of cyclobutrifluram (2.5 mg L-1), fluopyram (1.25 mg L-1) and fluensulfone (80 mg L-1) surpassed 85%, whereas hymexazol (640 mg L-1) was only 67%. Similar results were observed in growth cabinet experiments as well. The disease index (DI) and gall index (GI) were significantly decreased by all four nematicides compared to the control. However, the application of hymexazol did not yield a statistically significant difference in the egg masses index compared to the control, a finding which may be attributed to its potentially limited penetrability through the eggshell barrier. Overall, this study has demonstrated that all four nematicides effectively suppress M. enterolobii in sweetpotato, and this is the first report on the nematicidal activity of cyclobutrifluram and hymexazol against M. enterolobii.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Report and Molecular Variability of Belonolaimus longicaudatus Associated with Turfgrass in Maryland. 马里兰州首次报告与草坪有关的 Belonolaimus longicaudatus 及其分子变异性。

IF 1.4 4区生物学

Journal of nematology Pub Date : 2024-08-24 eCollection Date: 2024-03-01 DOI: 10.2478/jofnem-2024-0026

Benjamin Waldo, Andrea Skantar, Zafar Handoo, Shiguang Li, Alemayehu Habteweld, Fereshteh Shahoveisi

{"title":"First Report and Molecular Variability of Belonolaimus longicaudatus Associated with Turfgrass in Maryland.","authors":"Benjamin Waldo, Andrea Skantar, Zafar Handoo, Shiguang Li, Alemayehu Habteweld, Fereshteh Shahoveisi","doi":"10.2478/jofnem-2024-0026","DOIUrl":"10.2478/jofnem-2024-0026","url":null,"abstract":"Turfgrass is a crop used extensively in athletic fields and golf courses in Maryland. A soil sample collected in July 2023 from an athletic field in Baltimore County, Maryland, part of a turfgrass nematode survey, contained Belonolaimus longicaudatus. In the southeastern United States, B. longicaudatus is an economically important pathogen of warm season turfgrass. The density was four individuals/100 cm3 of soil, and no visual symptoms were observed in the bermudagrass field. Morphological features and morphometrics of males and females were consistent with B. longicaudatus and placed the Maryland population in a subclade that was geographically represented by populations from north and west Florida, Texas, and South Carolina. Sequencing of the internal transcribed spacer region ITS1 and ITS2 and 28S large ribosomal subunit D2-23 expansion region confirmed the species' identity. Phylogenetic trees and parsimony network analysis placed the Maryland isolate in a large grouping of B. longicaudatus populations including those from Alabama, Delaware, Florida, Indiana, Mississippi, South Carolina, and Texas. To our knowledge, this is the first report of B. longicaudatus in Maryland.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142117003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Field Applications of Fluorinated Nematicides for Meloidogyne enterolobii Management on Tomato. 田间应用含氟杀线虫剂治理番茄上的虫害。

IF 1.4 4区生物学

Journal of nematology Pub Date : 2024-08-16 eCollection Date: 2024-03-01 DOI: 10.2478/jofnem-2024-0030

R Castro-López, C A López-Orona, J A Martínez-Gallardo, M A Tirado-Ramírez, G Gómez, W Rubio-Aragón, J A Edeza-Urias, M C Villa-Medina

{"title":"Field Applications of Fluorinated Nematicides for Meloidogyne enterolobii Management on Tomato.","authors":"R Castro-López, C A López-Orona, J A Martínez-Gallardo, M A Tirado-Ramírez, G Gómez, W Rubio-Aragón, J A Edeza-Urias, M C Villa-Medina","doi":"10.2478/jofnem-2024-0030","DOIUrl":"10.2478/jofnem-2024-0030","url":null,"abstract":"Mexico is the 8th largest producer of tomatoes. Meloidogyne enterolobii is reported in Sinaloa, affecting tomato cultivars with genetic resistance to Meloidogyne spp. We aimed to evaluate field applications of fluopyram, fluensulfone, and fluazaindolizine treatments for managing M. enterolobii on tomatoes. Experiments were set on raised beds in a shade house. Nematicides were applied via drip irrigation. Under fluopyram treatment, M. enterolobii did not reduce the number of extra-large-size fruits. The number of large-size fruits with fluopyram and fluazaindolizine plus fluopyram treatments was also unaffected by M. enterolobii. Yield from the treatments fluopyram, fluazaindolizine plus fluopyram, and fluensulfone plus fluopyram was similar to the control treatment without M. enterolobii. Finally, fluazaindolizine plus fluopyram, fluopyram, and fluensulfone plus fluopyram treatments showed the highest reduction of root galling. We conclude that the fluopyram was more effective as an individual treatment. Pre-plant applications of fluensulfone and fluazaindolizine reduced the damage to the plant and the loss of yield; however, the complementary application of fluorinated nematicides improved the management of M. enterolobii in the tomato crop.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11330566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0