Journal of nematology最新文献

{"title":"JON 63rd Meeting 2024 Park City ABSTRACTS.","authors":"","doi":"10.2478/jofnem-2024-0036","DOIUrl":"https://doi.org/10.2478/jofnem-2024-0036","url":null,"abstract":"","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240036"},"PeriodicalIF":1.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11406932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome Announcement: Draft Genome Assembly of <i>Heterodera humuli</i> Generated Using Long-Read Sequencing.","authors":"Lester A Núñez-Rodríguez, Catherine L Wram, Cedar Hesse, Inga A Zasada","doi":"10.2478/jofnem-2024-0029","DOIUrl":"10.2478/jofnem-2024-0029","url":null,"abstract":"<p><p>The hop cyst nematode, <i>Heterodera humuli</i>, is the most common plant-parasitic nematode associated with hop worldwide. This study reports the draft genome of <i>H. humuli</i> generated on the PacBio Sequel IIe System with the ultra-low DNA input HiFi sequencing method, and the corresponding genome annotation. This genome resource will help further studies on <i>H. humuli</i> and other cyst nematodes.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240029"},"PeriodicalIF":1.4,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11366395/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxonomy of Tobrilidae species from the Alkaline Lakes of the western Nebraska Sandhills.","authors":"Peter G Mullin, Timothy Harris, Rebecca Higgins, Enakshy Dutta, Dorota L Porazinska, Kirsten Powers, Thomas Powers","doi":"10.2478/jofnem-2024-0025","DOIUrl":"10.2478/jofnem-2024-0025","url":null,"abstract":"<p><p>Six distinct COI mitochondrial Haplotype Groups (HG) are morphologically, ecologically, and genetically characterized from the aquatic nematode family Tobrilidae. Collection locations included the extreme habitats of the Alkaline Lakes in the western Nebraska Sandhills and the contaminated stream, Johnson Creek, bordering the AltEn 2021 catastrophic pesticide release near the village of Mead in eastern Nebraska. Maximum likelihood and genetic distance metrics supported the genetic integrity of the haplotype groups. Discriminant function analysis of COI haplotype group datasets of combined morphological characters and soil chemistry attributes for both male and female Tobrilidae were classified correctly in all but one case. Scanning electron microscopy revealed new details about amphid apertures, male supplements, and spicules. Partial 18S gene phylogeny suggests that the genus <i>Semitobrilus</i> may not be a member of the subfamily Neotobrilinae, and three specimens in the 226 tobrilid dataset provide evidence of incongruence between COI and 18S derived phylogenies. Given the strong signal provided by the environmental chemistry data, tobrilid mitochondrial haplotypes may well have value as environmental indicators.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240025"},"PeriodicalIF":1.4,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of nematicides for <i>Meloidogyne enterolobii</i> management in sweetpotato.","authors":"Jingwei Chen, Jukui Ma, Fangyuan Gao, Wei Tang, Dongjing Yang, Chengling Zhang, Zhao Liang, Yiping Xie, Houjun Sun","doi":"10.2478/jofnem-2024-0033","DOIUrl":"10.2478/jofnem-2024-0033","url":null,"abstract":"<p><p>Sweetpotato is an important crop whose roots are consumed by people worldwide. <i>Meloidogyne enterolobii</i> stands out as a highly deleterious variant among the species of root-knot nematode that causes significant damage in sweetpotato. In the present study, the activity of four nematicides against <i>M. enterolobii</i> was assessed both <i>in vitro</i> and in growth cabinet experiments. After 48 hours of exposure, fluopyram and cyclobutrifluram had a greater negative effect on the motility of <i>M. enterolobii</i> second-stage juveniles (J2s) compared to fluensulfone and hymexazol, with respective median effective concentration (EC₅₀) values of 0.204, 0.423, 22.335 and 216.622 mg L^-1. When <i>M. enterolobii</i> eggs were incubated for 72 hours at the highest concentration of each nematicides, the inhibitory hatching effect of cyclobutrifluram (2.5 mg L^-1), fluopyram (1.25 mg L^-1) and fluensulfone (80 mg L^-1) surpassed 85%, whereas hymexazol (640 mg L^-1) was only 67%. Similar results were observed in growth cabinet experiments as well. The disease index (DI) and gall index (GI) were significantly decreased by all four nematicides compared to the control. However, the application of hymexazol did not yield a statistically significant difference in the egg masses index compared to the control, a finding which may be attributed to its potentially limited penetrability through the eggshell barrier. Overall, this study has demonstrated that all four nematicides effectively suppress <i>M. enterolobii</i> in sweetpotato, and this is the first report on the nematicidal activity of cyclobutrifluram and hymexazol against <i>M. enterolobii</i>.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240033"},"PeriodicalIF":1.4,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report and Molecular Variability of <i>Belonolaimus longicaudatus</i> Associated with Turfgrass in Maryland.","authors":"Benjamin Waldo, Andrea Skantar, Zafar Handoo, Shiguang Li, Alemayehu Habteweld, Fereshteh Shahoveisi","doi":"10.2478/jofnem-2024-0026","DOIUrl":"10.2478/jofnem-2024-0026","url":null,"abstract":"<p><p>Turfgrass is a crop used extensively in athletic fields and golf courses in Maryland. A soil sample collected in July 2023 from an athletic field in Baltimore County, Maryland, part of a turfgrass nematode survey, contained <i>Belonolaimus longicaudatus</i>. In the southeastern United States, <i>B. longicaudatus</i> is an economically important pathogen of warm season turfgrass. The density was four individuals/100 cm³ of soil, and no visual symptoms were observed in the bermudagrass field. Morphological features and morphometrics of males and females were consistent with <i>B. longicaudatus</i> and placed the Maryland population in a subclade that was geographically represented by populations from north and west Florida, Texas, and South Carolina. Sequencing of the internal transcribed spacer region ITS1 and ITS2 and 28S large ribosomal subunit D2-23 expansion region confirmed the species' identity. Phylogenetic trees and parsimony network analysis placed the Maryland isolate in a large grouping of <i>B. longicaudatus</i> populations including those from Alabama, Delaware, Florida, Indiana, Mississippi, South Carolina, and Texas. To our knowledge, this is the first report of <i>B. longicaudatus</i> in Maryland.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240026"},"PeriodicalIF":1.4,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142117003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field Applications of Fluorinated Nematicides for <i>Meloidogyne enterolobii</i> Management on Tomato.","authors":"R Castro-López, C A López-Orona, J A Martínez-Gallardo, M A Tirado-Ramírez, G Gómez, W Rubio-Aragón, J A Edeza-Urias, M C Villa-Medina","doi":"10.2478/jofnem-2024-0030","DOIUrl":"10.2478/jofnem-2024-0030","url":null,"abstract":"<p><p>Mexico is the 8^th largest producer of tomatoes. <i>Meloidogyne enterolobii</i> is reported in Sinaloa, affecting tomato cultivars with genetic resistance to <i>Meloidogyne</i> spp. We aimed to evaluate field applications of fluopyram, fluensulfone, and fluazaindolizine treatments for managing <i>M. enterolobii</i> on tomatoes. Experiments were set on raised beds in a shade house. Nematicides were applied via drip irrigation. Under fluopyram treatment, <i>M. enterolobii</i> did not reduce the number of extra-large-size fruits. The number of large-size fruits with fluopyram and fluazaindolizine plus fluopyram treatments was also unaffected by <i>M. enterolobii</i>. Yield from the treatments fluopyram, fluazaindolizine plus fluopyram, and fluensulfone plus fluopyram was similar to the control treatment without <i>M. enterolobii</i>. Finally, fluazaindolizine plus fluopyram, fluopyram, and fluensulfone plus fluopyram treatments showed the highest reduction of root galling. We conclude that the fluopyram was more effective as an individual treatment. Pre-plant applications of fluensulfone and fluazaindolizine reduced the damage to the plant and the loss of yield; however, the complementary application of fluorinated nematicides improved the management of <i>M. enterolobii</i> in the tomato crop.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240030"},"PeriodicalIF":1.4,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11330566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxic Effects of the Trap Crop <i>Solanum sisymbriifolium</i> on the Hatch and Viability of <i>Globodera pallida</i>.","authors":"Lindsay Schulz, Inna Popova, Louise-Marie Dandurand","doi":"10.2478/jofnem-2024-0027","DOIUrl":"10.2478/jofnem-2024-0027","url":null,"abstract":"<p><p><i>Globodera pallida</i>, the pale cyst nematode, is a quarantined potato pest first found in Idaho in 2006. The containment and eradication of this economically devastating pest has been the focus of control since its discovery. <i>Globodera pallida</i> survives for 30+ years in soil and can cause up to 80% yield loss in susceptible potato varieties. Soil fumigants have been key to eradication efforts but many have been banned. Therefore, new control methods are needed. <i>Solanum sisymbriifolium</i> induces hatching but limits <i>G. pallida</i> reproduction and can be used as an alternative control measure. However, as <i>S. sisymbriifolium</i> has little economic value as a crop and its seeds are largely unavailable, it has not been widely adopted by potato producers. There is evidence that this plant kills the nematode by producing toxins, although this is poorly understood. Liquid-liquid extraction of <i>S. sisymbriifolium</i> leaf and stem tissues by hexane and 1-butanol reduced hatch by 49.5%, and 68.3%, respectively, compared with the potato root diffusate control. Many chemicals may be responsible for this toxic effect, including steroidal glycoalkaloids produced by plants in the Solanaceae family. The discovery of novel chemistries for nematicide development would be valuable for potato cyst nematode control.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240027"},"PeriodicalIF":1.4,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11310831/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicted distribution of <i>Metaparasitylenchus hypothenemi</i> (Tylenchida: Allantonematidae), parasite of the coffee berry borer.","authors":"M Simota-Ruiz, A Castillo, J Cisneros-Hernández, O Carmona-Castro","doi":"10.2478/jofnem-2024-0031","DOIUrl":"10.2478/jofnem-2024-0031","url":null,"abstract":"<p><p><i>Metaparasitylenchus hypothenemi</i> is an endoparasitic nematode of the coffee berry borer <i>Hypothenemus hampei</i>. The nematode has only been recorded across a limited geographical range in coffee-growing areas of southeastern Mexico. Because of its confined geographical distribution, the effect of altitude, temperature, and mean annual precipitation on <i>M. hypothenemi's</i> presence/absence in the Soconusco region of Mexico was investigated. The geographical distribution of this parasite was predicted based on current data, using geographical information systems (GIS), the MaxEnt algorithm, and historical data to improve the prediction accuracy for other Neotropical regions. In Soconusco, the presence of this parasite is directly related to annual precipitation, especially in the areas with the highest annual rainfall (4000 - 4700 mm/year). Four species distribution models were generated for the Neotropical region with environmental variables for sites with parasite presence data, predicting a range of possible distribution with a high probability of occurrence in southeastern Mexico and southwestern Guatemala and a low probability in areas of Central and South America. Characterization of the abiotic habitat conditions suitable for <i>M. hypothenemi</i> development allows us to predict its distribution in the Neotropics and contributes to our understanding of its ecological relationship with environmental variables.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240031"},"PeriodicalIF":1.4,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11305401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and Molecular Analysis of Two Mycophagous Nematodes, <i>Aphelenchoides bicaudatus</i> and <i>A. rutgersi</i> (Nematoda: Aphelenchoididae) from Florida Strawberry.","authors":"Clemen J Oliveira, Sergei A Subbotin, Johan A Desager, Paul Dahlin, Silvia Vau, Renato N Inserra","doi":"10.2478/jofnem-2024-0021","DOIUrl":"10.2478/jofnem-2024-0021","url":null,"abstract":"<p><p>From 2016 to 2021, nematode surveys in Florida strawberry fields revealed several species of foliar nematodes (<i>Aphelenchoides</i> spp.). <i>Aphelenchoides besseyi sensu stricto</i> was detected only in 2016 and 2017 on photosynthetic strawberry leaves/buds, but other not well characterized populations of <i>Aphelenchoides</i> sp. were found on declining/dessicated leaves. Morphological analyses showed that these samples of <i>Aphelenchoides</i> sp. consisted of <i>A. bicaudatus</i>, a species detected in Florida for the first time, and <i>A. rutgersi</i>, a species previously reported in Florida from the citrus rhizosphere. These two species differed from <i>A. besseyi</i> in the shape of their tail terminus: bifurcate in <i>A. bicaudatus</i>; mucronate with a ventral thin mucro in <i>A. rutgersi</i>; and stellate in <i>A. besseyi</i>. One population each of these species was used for morphological and molecular analyses after being reared on <i>Monilinia fructicola</i>. Body and tail length differences were observed among Florida <i>A. bicaudatus</i> and other populations from the Far East and South Africa. Phylogenetic analyses of the rRNA gene sequences showed that Florida <i>A. bicaudatus</i> grouped with those of species from South Korea, Taiwan, and the Netherlands and several other populations listed as <i>Aphelenchoides</i> sp. from Brazil, Costa Rica, and Japan, which were considered as representatives of <i>A. bicaudatus</i> in this study. Similarly, sequences of Florida <i>A. rutgersi</i> grouped with those from environmental samples in Japan and North Carolina, which were listed as <i>Aphelenchoides</i> sp. and were considered as representatives of <i>A. rutgersi</i> in this study. Photosynthetic strawberry leaf samples were free from both <i>A. bicaudatus</i> and <i>A. rutgersi</i>, indicating that these two species did not damage strawberry. They were associated with desiccated leaves and/or propagative stolons, usually infected by fungi, confirming that they are mycetophagous under field conditions in this study. Results of soybean leaf inoculation on moist filter paper containing <i>A. bicaudatus</i> specimens showed that this species could become phytophagous under artificial conditions. Nematodes penetrated the leaf epidermis and migrated into the mesophyll causing leaf tissue discoloration/necrosis, which remained localized within the infested area. Soybean leaf damage was almost negligible, and no nematode reproduction was observed in the inoculated soybean areas.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240021"},"PeriodicalIF":1.4,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11224714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinase Polymerase Amplification assay for detection of the British root-knot nematode, <i>Meloidogyne artiellia</i>.","authors":"Sergei A Subbotin, Juan E Palomares-Rius, Pablo Castillo","doi":"10.2478/jofnem-2024-0023","DOIUrl":"10.2478/jofnem-2024-0023","url":null,"abstract":"<p><p>Recombinase polymerase amplification (RPA) is an isothermal <i>in vitro</i> nucleic acid amplification technique that has been adopted for simple, robust, rapid, reliable diagnostics of nematodes. In this study, the real-time RPA assay and RPA assay combined with lateral flow dipsticks (LF-RPA) have been developed targeting the ITS rRNA gene of the British root-knot nematode, <i>Meloidogyne artiellia</i>. The assay provided specific and rapid detection of this root-knot nematode species from crude nematode extracts without a DNA extraction step with a sensitivity of 0.125 second-stage juvenile (J2) specimen per a reaction tube for real-time RPA during 11 min and a sensitivity of 0.5 J2 specimens per a reaction tube for LF-RPA during 25 min. The RPA assays were validated with a wide range of non-target root-knot nematodes. The LF-RPA assay has great potential for nematode diagnostics in the laboratory having minimal available equipment.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240023"},"PeriodicalIF":1.4,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11224650/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}