Journal of nematology最新文献_第3页

Distribution and characterization of the root-knot nematode, Meloidogyne javanica (Treub, 1885) Chitwood, 1949 (Meloidogynidae) isolates, in some regions of two main pistachio growing provinces of Iran. 根结线虫，Meloidogyne javanica （Treub, 1885）和Chitwood， 1949 （Meloidogynidae）分离株在伊朗两个开心果主要种植省部分地区的分布和特征。

IF 2 4区生物学

Journal of nematology Pub Date : 2026-05-29 eCollection Date: 2026-01-01 DOI: 10.2478/jofnem-2026-0015

Fatemeh Shekari Mahoonaki, Esmat Mahdikhani Moghadam, Mohammad Reza Atighi, Mohammad Zakiaghl, Fatemeh Bazeghi, Majid Pedram

{"title":"Distribution and characterization of the root-knot nematode, Meloidogyne javanica (Treub, 1885) Chitwood, 1949 (Meloidogynidae) isolates, in some regions of two main pistachio growing provinces of Iran.","authors":"Fatemeh Shekari Mahoonaki, Esmat Mahdikhani Moghadam, Mohammad Reza Atighi, Mohammad Zakiaghl, Fatemeh Bazeghi, Majid Pedram","doi":"10.2478/jofnem-2026-0015","DOIUrl":"10.2478/jofnem-2026-0015","url":null,"abstract":"Pistachio is one of the most economically important horticultural crops worldwide, and its yield is annually reduced due to damages caused by root-knot nematodes (RKNs). To identify the species of RKNs associated with pistachio trees in Kerman and Khorasan Razavi provinces of Iran, 84 localities were investigated and 37 isolates of the predominant species, Meloidogyne javanica, were identified based on the perineal pattern of females and species-specific primers Fjav and Rjav. Eight isolates (KS1-1, KS1-10, KN7-1, KR3-1, KR8-1, KSh1-1, KhF1-4, and KhF2-1) were further characterized using morphological, morphometric, and molecular data (Small subunit [SSU] rDNA, COII-16S, and NADH dehydrogenase subunit 5 [Nad5] mtDNA sequences). The SSU sequences of these eight isolates showed over 98% identity with several sequences of tropical Meloidogyne species. The COII-16S and Nad5 sequences displayed 99.63 and 100% identity with other available M. javanica sequences, respectively. This study provided, for the first time, comprehensive morphometric, molecular, and geographical distribution data of M. javanica isolates parasitizing pistachio in two main pistachio-producing provinces of Iran. Based on the results of this research, the prevalence of M. javanica in the pistachio orchards of the studied regions was 44.04%, indicating its widespread distribution. Rafsanjan in Kerman province and Mahvelat in Khorasan Razavi province were two highly infested cities based on the present study. Therefore, regular monitoring is essential for effective management of this pest.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"58 1","pages":"189-207"},"PeriodicalIF":2.0,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13221253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148138476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Draft Genomes of Heterorhabditis bacteriophora and its symbiont from Southwestern China. 中国西南嗜菌异habditis bacteriophora及其共生体基因组草图。

IF 2 4区生物学

Journal of nematology Pub Date : 2026-05-28 eCollection Date: 2026-01-01 DOI: 10.2478/jofnem-2026-0016

Yanjun Man, Zhen Wang, Zhidong Wang, Ning Yang, Sizhuo Zhang, Ziduan Han

引用次数: 0

Comparative metabarcoding study performed on the mock soil nematode communities, composed of DESS-preserved and freshly extracted nematodes. 对dess保存和新鲜提取的模拟土壤线虫群落进行了比较元条形码研究。

IF 2 4区生物学

Journal of nematology Pub Date : 2026-05-16 eCollection Date: 2026-01-01 DOI: 10.2478/jofnem-2026-0014

Katarzyna Rybarczyk-Mydłowska, Mohammed Ahmed, Grażyna Winiszewska

{"title":"Comparative metabarcoding study performed on the mock soil nematode communities, composed of DESS-preserved and freshly extracted nematodes.","authors":"Katarzyna Rybarczyk-Mydłowska, Mohammed Ahmed, Grażyna Winiszewska","doi":"10.2478/jofnem-2026-0014","DOIUrl":"10.2478/jofnem-2026-0014","url":null,"abstract":"Amplicon sequencing-based community analyses offer an attractive alternative to microscopy-based nematode identification approaches where expert knowledge is required. However, the potential of analysing DESS-preserved samples in metabarcoding studies remained underexplored. Moreover, previous studies often omitted certain representative groups of nematodes. To fill this gap, a nematode metabarcoding experiment was conducted on two types of mock community sample sets: DESS pretreated and freshly acquired, both of them covering taxa of various feeding strategies. Additionally, the performance of two, 18S and 28S rDNA-based (the D2A/D2 primer pair for the first time in such studies), polymerase chain reaction primer combinations, and the efficiency of two DNA isolation methods were compared. The results of this study show that the DESS preservative can be successfully used in qualitative metabarcoding studies of soil nematode communities. All 24, initially morphologically identified, nematode taxa were recovered from the DESS-treated and fresh nematode samples, using both rDNA primer combinations and both DNA isolation methods tested. Each of the two isolation methods used had stronger effect on read recovery of slightly different taxa.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"58 1","pages":"165-185"},"PeriodicalIF":2.0,"publicationDate":"2026-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13183384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147972940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Helicotylenchus coreanus n. sp. (Nematoda: Hoplolaimidae): a new spiral nematode associated with Abies koreana Wils. from the Republic of Korea. （线虫纲：圆线虫科）：与朝鲜冷杉有亲缘关系的一种新螺旋线虫。来自大韩民国。

IF 2 4区生物学

Journal of nematology Pub Date : 2026-05-15 eCollection Date: 2026-01-01 DOI: 10.2478/jofnem-2026-0013

Abraham Okki Mwamula, Chang-Hwan Bae, Suk-Hyung Ko, Yeong-Don Lee, Eun-Young Yim, Seung-Hoon Lee, Dong Woon Lee

{"title":"Helicotylenchus coreanus n. sp. (Nematoda: Hoplolaimidae): a new spiral nematode associated with Abies koreana Wils. from the Republic of Korea.","authors":"Abraham Okki Mwamula, Chang-Hwan Bae, Suk-Hyung Ko, Yeong-Don Lee, Eun-Young Yim, Seung-Hoon Lee, Dong Woon Lee","doi":"10.2478/jofnem-2026-0013","DOIUrl":"10.2478/jofnem-2026-0013","url":null,"abstract":"A new species of the genus Helicotylenchus extracted from the rhizospheric soils of dead Abies koreana was described using morphological characters, morphometrics, and molecular DNA barcodes. Helicotylenchus coreanus n. sp. is characterized by its lateral fields with non-areolated bands, modified at vulval region by widening on vulval side, inner lateral field incisures on tail end fused distally into a Y- or U-shape, phasmids located 0-7 annuli posterior to anal opening, rarely three annuli anterior to anal opening, lip region hemispherical, continuous with body contour, marked by six or seven distinct annuli, stylet 3.4-4.0 times as long as lip region diam., stylet knobs rounded to flattened, spermatheca rounded, empty, tail mostly longer than anal body diam., with 16-31 annuli; rarely less than 15 annuli on ventral contour, tail terminal region with a well-demarcated hyaline portion, 3.0-5.5 μm long, terminus notched, sometimes with two small notches; in the more conoid individuals, terminus is truncate or rounded. The new species was morphologically compared with the six related species of the genus, including H. indenticaudatus, H. holguinensis, H. parapteracercus, H. ussuriensis, H. belli, and H. oscephalus. The phylogenetic relationships among species were reconstructed using 18S-rRNA, 28S-rRNA, and COI gene sequences. Inferences from the more informative 28S-rRNA and COI genes supported sister relations of Helicotylenchus coreanus n. sp. with H. oscephalus.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"58 1","pages":"150-164"},"PeriodicalIF":2.0,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13178048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147963900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of Deladenus longicaudatus n. sp. (Sphaerularioidea: Neotylenchidae) from Mazandaran province, northern Iran: A morphological and molecular phylogenetic study. 伊朗北部马赞达兰省长尾Deladenus longicaudatus n. sp.描述（球藻总科：新球藻科）：形态和分子系统发育研究。

IF 2 4区生物学

Journal of nematology Pub Date : 2026-05-07 eCollection Date: 2026-01-01 DOI: 10.2478/jofnem-2026-0002

Mehdi Salemi, Farahnaz Jahanshahi Afshar, Mohammad Reza Atighi, Majid Pedram

{"title":"Description of Deladenus longicaudatus n. sp. (Sphaerularioidea: Neotylenchidae) from Mazandaran province, northern Iran: A morphological and molecular phylogenetic study.","authors":"Mehdi Salemi, Farahnaz Jahanshahi Afshar, Mohammad Reza Atighi, Majid Pedram","doi":"10.2478/jofnem-2026-0002","DOIUrl":"10.2478/jofnem-2026-0002","url":null,"abstract":"Deladenus longicaudatus n. sp. was recovered from decaying wood of a broad-leaf forest tree in the forests of Javaherdeh, Mazandaran province, northern Iran, and is herein described and illustrated based upon morphological, morphometric, and molecular characters. The new species is mainly characterized by its small to medium body size of free-living mycetophagous females (523-682 µm long) with an elongate conical tail (c = 9.3-11.0 and c' = 3.9-5.3), a relatively anterior vulval position (V = 84.5-89.0%), the pharyngo-intestinal junction at the level of the nerve ring, the secretory-excretory pore (S-E pore) posterior to the nerve ring, and at the same level as the hemizonid. It is further characterized by seven incisures in the lateral fields, a short stylet (5.0-6.5 µm), small lateral vulval flaps, and absence of a postvulval uterine sac (PUS). By having similarities in tail shape, c' ratio greater than 3, posterior position of the S-E pore and hemizonid to the nerve ring, and lack of PUS, the new species is comparable to four known species of the genus, namely, D. gilanica, D. hyrcanus, D. zyzyphus, and D. parvus. It is furthermore typologically comparable to mycetophagous phase of Hexatylus vigissi. In molecular phylogenetic analyses using partial small and large subunit (SSU and LSU D2-D3) rDNA sequences, the SSU sequences of the new species formed a moderately supported clade with sequences of Howardula spp. In LSU phylogeny, the newly generated sequences of the new species formed a clade with corresponding sequence of D. hyrcanus.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"58 1","pages":"126-136"},"PeriodicalIF":2.0,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13154951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147868722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Papaya seed extract for management of Radopholus similis on Anthurium. 番木瓜种子提取物在红掌上管理的应用。

IF 2 4区生物学

Journal of nematology Pub Date : 2026-05-05 eCollection Date: 2026-01-01 DOI: 10.2478/jofnem-2026-0003

Lauren Braley, Roxana Myers, Roshan Paudel, Wei Wen Su, Koon-Hui Wang

{"title":"Papaya seed extract for management of Radopholus similis on Anthurium.","authors":"Lauren Braley, Roxana Myers, Roshan Paudel, Wei Wen Su, Koon-Hui Wang","doi":"10.2478/jofnem-2026-0003","DOIUrl":"10.2478/jofnem-2026-0003","url":null,"abstract":"Anthurium cut flowers are an economically important ornamental crop in Hawai'i, but their production is threatened by the burrowing nematode, Radopholus similis, which reduces yield and increases production costs. This study evaluated the nematicidal potential of crude extract from ground papaya seed (PGS CE), a local agricultural by-product that contains glucosinolates that hydrolyze into benzyl isothiocyanate, active against R. similis. Greenhouse trials demonstrated that monthly drenches with 0.5 or 1.0% PGS CE significantly reduced R. similis population densities in both roots and cinder media, decreased root lesion severity, and increased leaf production without phytotoxicity or adverse effects on free-living bacterivorous nematodes. While 1.0% PGS CE slightly reduced plant biomass, 0.5% PGS CE improved plant growth and flower production. Regression analysis supported the link between nematode suppression and enhanced anthurium health. Thus, PGS CE presents a sustainable, locally available, and effective biofumigant for nematode management in anthurium, warranting further optimization and future field-scale validation.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"58 1","pages":"116-125"},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13148781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147839156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of Pristionchus endotocus n. sp., a new obligately bagging androdioecious species from the Philippines. 菲律宾专装袋雄性异雄新种内托棱蚊的描述。

IF 2 4区生物学

Journal of nematology Pub Date : 2026-04-28 eCollection Date: 2026-01-01 DOI: 10.2478/jofnem-2026-0007

Berke Güner, Natsumi Kanzaki, Christian Weiler, Christian Rödelsperger, Nanette Hope N Sumaya, Ralf J Sommer, Matthias Herrmann

{"title":"Description of Pristionchus endotocus n. sp., a new obligately bagging androdioecious species from the Philippines.","authors":"Berke Güner, Natsumi Kanzaki, Christian Weiler, Christian Rödelsperger, Nanette Hope N Sumaya, Ralf J Sommer, Matthias Herrmann","doi":"10.2478/jofnem-2026-0007","DOIUrl":"10.2478/jofnem-2026-0007","url":null,"abstract":"Pristionchus pacificus is an important model organism with a well-developed suite of molecular tools and a genomic dataset. Studies that integrate population genetics, ecology, and evo-devo in P. pacificus are supported by an extensive and robust phylogeny consisting of more than 50 Pristionchus species and close to 3,000 strains. Asia by and large has emerged as the biodiversity hot spot of the genus, becoming the focus of recent sampling efforts for Pristionchus isolates. Here, we describe a new androdioecious species of Pristionchus discovered in recent samplings in the Philippines based on a combination of molecular markers, morphological and morphometric data, and mating experiments. All strains of the new species were collected from Scarabeoid beetles in the Philippines and are basal to a sub-clade of the maupasi clade. Pristionchus endotocus n. sp. exhibits constant bagging and a strong bias toward the stenostomatous morph under laboratory conditions and might therefore provide an additional reference point for life history trait studies in Pristionchus.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"58 1","pages":"78-93"},"PeriodicalIF":2.0,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13127830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nematophagous Hyalorbilia spp. isolated from Heterodera schachtii in California parasitize Heterodera glycines. 从加州沙氏异线虫分离的噬线虫透明菌寄生于甘氨酸异线虫。

IF 2 4区生物学

Journal of nematology Pub Date : 2026-04-28 eCollection Date: 2026-01-01 DOI: 10.2478/jofnem-2026-0009

Jennifer Smith Becker, James Borneman, Jiue-In Yang, Jörn Ole Becker

{"title":"Nematophagous Hyalorbilia spp. isolated from Heterodera schachtii in California parasitize Heterodera glycines.","authors":"Jennifer Smith Becker, James Borneman, Jiue-In Yang, Jörn Ole Becker","doi":"10.2478/jofnem-2026-0009","DOIUrl":"10.2478/jofnem-2026-0009","url":null,"abstract":"The goal of this study is to test whether parasitic Hyalorbilia spp., isolated from sugarbeet cyst nematodes (Heterodera schachtii) in California, could also suppress soybean cyst nematodes (H. glycines). Three H. oviparasitica clade strains, DoUCR50, HsImV27, and ARF18-L, were cultured in powdered peat for use in greenhouse nematode suppression tests. Soils were amended with 500 CFU/cm3 of each fungal strain or autoclaved peat inoculum as a control, seeded with soybeans (Glycine max cv. Williams 82), and 3 weeks later, inoculated with 250 J2 of H. glycines. After 1,260 degree days, cysts and females were collected by flotation sieving and enumerated. DoUCR50, HsImV27, and ARF18-L reduced cysts and females of H. glycines by 73, 87, and 0%, respectively. Egg parasitism was tested by incubating eggs of H. schachtii and H. glycines on water agar cultures of DoUCR50 and HsImV27. Individual eggs of H. schachtii or H. glycines were parasitized by DoUCR50 at rates of 15 or 13%, respectively. Eggs placed in pairs were parasitized at 38 and 43%, while eggs placed in groups of four were parasitized at 70 and 63%, respectively. HsImV27 parasitized fewer than 10% of eggs of either nematode, regardless of egg group size. Both DoUCR50 and HsImV27 were observed to parasitize white females of H. glycines and H. schachtii in tissue culture.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"58 1","pages":"94-103"},"PeriodicalIF":2.0,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13127828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth and defense enzyme responses of grafted cucurbit crops to varying initial populations of Meloidogyne incognita. 嫁接瓜类作物对不同初始种群的生长和防御酶反应。

IF 2 4区生物学

Journal of nematology Pub Date : 2026-04-28 eCollection Date: 2026-01-01 DOI: 10.2478/jofnem-2026-0006

Hosny H Kesba, Sherif M El-Ganainy, Nourhan M M Hassnin, Ali H H Ali

{"title":"Growth and defense enzyme responses of grafted cucurbit crops to varying initial populations of Meloidogyne incognita.","authors":"Hosny H Kesba, Sherif M El-Ganainy, Nourhan M M Hassnin, Ali H H Ali","doi":"10.2478/jofnem-2026-0006","DOIUrl":"10.2478/jofnem-2026-0006","url":null,"abstract":"Root-knot nematode (Meloidogyne incognita) is among the most serious constraints to cucurbit crop production worldwide. This study evaluated the effects of different initial population densities (0, 1,000, 2,000, 4,000, and 8,000 second-stage juveniles [J 2]/plant) on nematode development, reproduction, plant growth, and defense-related enzyme activities in watermelon (Citrullus lanatus, cv. Aswan F1) and sweet melon (Cucumis melo, cv. Faransawy) hybrids, their grafted combinations, and the resistant rootstocks 6001 F1 and BS F1. Plants were grown in sterilized sandy-loam soil under greenhouse conditions, and nematode parameters, including gall index, females per root system, egg masses per root, final population (Pf), reproduction factor, penetration rate, and reproduction rate were assessed 60 days after inoculation. Both host genotype and inoculum density significantly (P < 0.001) affected nematode reproduction and plant growth traits. Resistant rootstocks and their grafted scions consistently exhibited lower Pf/Pi ratios, fewer galls, reduced shoot weight losses, and significantly higher peroxidase and polyphenol oxidase activities compared to susceptible cultivars. These findings demonstrate that combining resistant rootstocks with grafting can effectively suppress M. incognita multiplication and associated growth losses, while enzyme activity profiling provides a useful biochemical indicator of partial resistance in cucurbit breeding and management programs.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"58 1","pages":"104-115"},"PeriodicalIF":2.0,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13127829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beech leaf disease symptom detection using deep learning and computer vision tools. 利用深度学习和计算机视觉工具检测山毛榉叶病症状。

IF 2 4区生物学

Journal of nematology Pub Date : 2026-04-27 eCollection Date: 2026-01-01 DOI: 10.2478/jofnem-2026-0011

Benjamin D Waldo, Paulo Vieira, Matthew A Borden, Shiguang Li

{"title":"Beech leaf disease symptom detection using deep learning and computer vision tools.","authors":"Benjamin D Waldo, Paulo Vieira, Matthew A Borden, Shiguang Li","doi":"10.2478/jofnem-2026-0011","DOIUrl":"10.2478/jofnem-2026-0011","url":null,"abstract":"Beech leaf disease (BLD) has rapidly emerged as a significant threat to forests across the eastern United States and Canada, and early detection is a major challenge. Current surveillance relies on visual identification of characteristic leaf banding, a method that can miss early infections. To address this limitation, we developed deep learning models capable of distinguishing between BLD symptomatic leaves and asymptomatic leaves. A primary dataset of symptomatic and asymptomatic leaves collected in Maryland (Dataset I) was used for model development, and an independent set of images collected in North Carolina, Ohio, and New England (Dataset II) provided real-world validation. In Dataset I model testing, EfficientNetV2-Small was the most accurate model (100%), followed by ResNet50 (99.32%), MobileNetV3-Large (97.95%), and InceptionV3 (94.88%). Independent testing on Dataset II also identified EfficientNetV2-Small as the most accurate model (96.55%). Grad-CAM visualizations confirmed that EfficientNetV2-Small focused on banded regions of BLD leaves that are a main characteristic of the disease. These findings demonstrate the potential of deep learning and computer vision approaches to support more efficient monitoring of BLD in forested regions.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"58 1","pages":"66-77"},"PeriodicalIF":2.0,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13133654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0