Journal of nematology最新文献

{"title":"Geographical distribution and phoretic associations of the viviparous nematode <i>Tokorhabditis atripennis</i> with <i>Onthophagus</i> dung beetles in Japan.","authors":"Yuya Ikeda, Yuto Koike, Ryoji Shinya, Koichi Hasegawa","doi":"10.2478/jofnem-2024-0013","DOIUrl":"10.2478/jofnem-2024-0013","url":null,"abstract":"<p><p>Viviparity is generally considered to be rare in animals. In nematodes, only six species of Rhabditida are viviparous. Five of these species have been identified in association with <i>Onthophagus</i> dung beetles, with <i>Tokorhabditis atripennis</i> being repeatedly isolated from the dung beetle <i>Onthophagus atripennis</i> in Japan. <i>T. atripennis</i> is easy to culture in a laboratory setting, and its host, <i>O. atripennis</i>, is distributed all over Japan. Therefore, <i>T. atripennis</i> is an ideal candidate for ecological and evolutionary studies on viviparity. However, the extent of their distribution and relationship with dung beetles, as well as habitats, remain unclear. In the present study, we conducted field surveys and successfully isolated 27 strains of viviparous nematodes associated with tunneler dung beetles from various regions of Japan, all of which were identified as <i>T. atripennis</i>. <i>T. atripennis</i> exhibited a strong association with <i>Onthophagus</i> dung beetles, especially <i>O. apicetinctus</i> and <i>O. atripennis</i>. And it was predominantly found in specific anatomical locations on the beetle bodies, such as the 'groove between pronotum and elytron' and the 'back of the wings'. Our findings suggest that <i>Onthophagus</i> species are the primary hosts for <i>T. atripennis</i>, and <i>T. atripennis</i> exhibits a close relationship with the living environments of tunneler beetles. This association may play a significant role in the evolution of viviparity in nematodes.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11044808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140855300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vertical Migration of Second-stage Juveniles of <i>Meloidogyne enterolobii</i> as Influenced by Temperature and Host.","authors":"Ana Karina S Oliveira, Elvira M R Pedrosa, Diego A H S Leitão, Janete A Brito, Ênio F de F Silva, Donald W Dickson","doi":"10.2478/jofnem-2024-0012","DOIUrl":"10.2478/jofnem-2024-0012","url":null,"abstract":"<p><p>Infective second-stage juveniles (J2) of <i>Meloidogyne</i> spp. migrate towards host roots, which depends on several factors, including root exudates and soil temperature. Although <i>Meloidogyne enterolobii</i> is a highly virulent nematode that affects major agricultural crops worldwide, there is limited ecological data about it. The objective of this study was to determine the J2 migration pattern vertically in 14-cm long segmented soil columns towards tomato (<i>Solanum lycopersicum</i>) and marigold (<i>Tagetes patula</i>) roots, each grown at two soil temperatures (20 or 26ºC). Bottomless cups with tomatoes or marigolds were attached to the top of each column; cups with no plants were used as untreated controls. Juveniles (1,000/column) were injected into a hole located 1 cm from the bottom of each column. The apparatuses were placed in growth chambers at 20 or 26ºC, and J2 were allowed to migrate for 3, 6, 9, or 12 days after injection (DAI). At each harvest, J2 were extracted from each ring of the columns and counted to compare their distribution, and root systems were stained to observe root penetration. <i>M. enterolobii</i> migrated over 13 cm vertically 3 DAI regardless of temperature, even without plant stimuli. The vertical migration was greater at 26ºC, where 60% of active J2 were found at distances >13 cm at 12 DAI. Temperature did not affect root penetration. Overall, a greater number of J2 was observed in tomato roots, and root penetration increased over time.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11033720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140865005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cotton host resistance as a tool for managing <i>Rotylenchulus reniformis</i> in Louisiana.","authors":"Tristan T Watson","doi":"10.2478/jofnem-2024-0014","DOIUrl":"10.2478/jofnem-2024-0014","url":null,"abstract":"<p><p>The reniform nematode, <i>Rotylenchulus reniformis</i>, is a major yield-limiting pest of upland cotton (<i>Gossypium hirsutum</i>) in the United States that has been steadily increasing in incidence in many states. Reniform nematode-resistant cotton cultivars have recently become commercially available for cotton producers; however, few field trials have evaluated their efficacy as a nematode management tool. The aim of this study was to evaluate reniform nematode population development, plant growth, and seed cotton yield of reniform nematode-resistant cotton cultivars in two nematode-infested fields in Louisiana. Replicated small-plot field trials were conducted in St. Joseph, LA (NERS field) and Winnsboro, LA (MRRS field) during the 2022 and 2023 growing seasons. In 2022, cultivars evaluated included: (1) DP 1646 B2XF (susceptible/tolerant), (2) DP 2141NR B3XF (resistant), (3) PHY 332 W3FE (resistant), (4) PHY 411 W3FE (resistant), and (5) PHY 443 W3FE (resistant). In 2023, an additional susceptible cotton cultivar, PHY 340 W3FE, was also included. All nematode-resistant cotton cultivars evaluated provided suppression of reniform nematode population development relative to that of the susceptible cotton cultivars, with suppression of nematode soil population densities at harvest ranging from 49 - 81% relative to DP 1646 B2XF. The resistant cultivar PHY 411 W3FE provided the most consistent suppression of reniform nematode population development, reducing reniform nematode soil population densities at harvest in both field locations and both trial years. In contrast, DP 2141NR B3XF only reduced soil population densities at harvest in the NERS field in 2023. Despite relatively consistent nematode suppression and improvements in plant vigor ratings and canopy coverage associated with the resistant cotton cultivars, a yield increase was only observed with PHY 332 W3FE and PHY 411 W3FE planted at the NERS field in 2023. Despite strong resistance to reniform nematode in the evaluated cotton cultivars, nematode soil population densities still increased during the growing season in plots planted with resistant cotton cultivars, emphasizing the need for additional management tactics to use alongside host resistance. This study indicates that new reniform nematode-resistant cotton cultivars show promising potential to reduce nematode population development during the growing season in Louisiana.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11033718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140855236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of <i>Nothotylenchus savadkoohensis</i> n. sp. (Rhabditida, Anguinidae) from Iran based on morphological and molecular data.","authors":"Soheila Rezaei, Ebrahim Pourjam, Mohammad Reza Atighi, Majid Pedram","doi":"10.2478/jofnem-2024-0015","DOIUrl":"10.2478/jofnem-2024-0015","url":null,"abstract":"<p><p><i>Nothotylenchus savadkoohensis</i> n. sp. was recovered from rotten wood samples of an unidentified forest tree in the Mazandaran province and described herein. It is mainly characterized by an elongated conoid tail ending in a sharply pointed tip and four lines in the lateral field. Females of the new species have 379-662 μm long bodies with 5.8-6.9 μm long stylets ending in fine posteriorly sloping knobs, the metacorpus not valvate, the pharyngeal bulb slightly overlapping the intestine, and the vulva at 76.5-84.0% of body length. Males are also common and have 13.0-14.5 μm long spicules and bursa cloacal. By having an elongated conoid tail and four lines in the lateral field, the new species comes close to four known species, namely <i>N. acris, N. acutus, N. antricolus</i>, and <i>N. truncatus</i>. The morphological differences between the new species and the abovementioned species are discussed. The new species was sequenced for its D2-D3 segment of LSU and ITS rDNA regions. In the LSU phylogenetic tree, the currently available LSU sequences of the genus <i>Nothotylenchus</i> occupied distant placements from each other and the LSU sequence of the new species formed clade with a sequence assigned to <i>Neotylenchus</i> sp. In ITS phylogeny, the newly generated sequence of the new species formed a clade with a clade that includes sequences of <i>Ditylenchus</i> sp. and <i>Neomisticius platypi</i> and <i>N. variabilis</i>.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11033712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Weed Species for Host Status to the Root-Knot Nematodes <i>Meloidogyne enterolobii</i> and <i>M. incognita</i> Race 4.","authors":"Tanner Schwarz, Chitra, Katherine Jennings, Adrienne Gorny","doi":"10.2478/jofnem-2024-0017","DOIUrl":"10.2478/jofnem-2024-0017","url":null,"abstract":"<p><p>Weeds that compete with valuable crops can also host plant-parasitic nematodes, acting as a source of nematode inoculum in a field and further damaging crops. The host status of 10 weed species commonly found in North Carolina, USA, was determined for the root-knot nematodes <i>Meloidogyne enterolobii</i> and <i>M. incognita</i> race 4 in the greenhouse. Each weed species was challenged with 5,000 eggs/plant of either <i>M. enterolobii</i> or <i>M. incognita</i> race 4, with five replicate plants per treatment in two separate greenhouse trials. Root galling severity and total number of nematode eggs per root system were recorded 60 days after inoculation. Reproduction factor (Rf = final nematode population/initial nematode population) was calculated to determine the host status of each weed species to <i>M. enterolobii</i> and <i>M. incognita</i> race 4. Four weed species (<i>Datura stramonium, Digitaria sanguinalis, Senna obtusifolia,</i> and <i>Cyperus esculentus</i>) were poor hosts (Rf < 1) to both nematode species, and roots of these weed plants did not display galling. Four weed species (<i>Ipomoea hederacea, Amaranthus palmeri, Portulaca pilosa,</i> and <i>Ipomoea lacunosa</i>) were hosts (Rf > 1) to both nematode species, and all had observable root gall formation. <i>Sida rhombifolia</i> and <i>Cyperus rotundus</i> were poor hosts to <i>M. enterolobii</i> but susceptible hosts to <i>M. incognita</i>. This study documents a differential host status of some common weeds to <i>M. enterolobii</i> and <i>M. incognita</i> race 4, and these results highlight the necessity of managing root-knot nematodes through controlling weeds in order to protect valuable crops.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11033719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140870546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of <i>Hypothenemus hampei</i> Ferrari (Coleoptera: Curculionidae: Scolytinae) parasitized by the nematode <i>Metaparasitylenchus hypothenemi</i> Poinar (Tylenchida: Allantonematidae) to different colors of light.","authors":"M Simota-Ruiz, A Castillo-Vera","doi":"10.2478/jofnem-2024-0011","DOIUrl":"10.2478/jofnem-2024-0011","url":null,"abstract":"<p><p><i>Metaparasitylenchus hypothenemi</i> is a nematode that naturally parasitizes <i>Hypothenemus hampei</i> in a coffee-producing region in Chiapas, Mexico. This study investigated changes in the attraction of parasitized borers to light. We compared the attraction of adult <i>H. hampei</i> females (parasitized and uninfected) to 14 different light wavelengths (350-670 nm) with a control (570 nm, yellow) under laboratory conditions. The response ranges of non-parasitized and parasitized borers were 370-650 nm and 340-650 nm, respectively. The attraction curve showed a similar shape in both borer groups (parasitized and non-parasitized), but a wide wavelength range (380-590 nm) attracted more parasitized than non-parasitized borers. The maximum response of the uninfected borers occurred at 520 nm (green), while parasitized borers exhibited three response peaks (380 nm, violet; 460 nm, blue; 520 nm, green). Parasitized borers were significantly more attracted to green light (520 nm) than to the control. The altered attraction to light in borers parasitized by <i>M. hypothenemi</i> is discussed from the perspective of possible host manipulation and the natural prevalence of this parasite.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11001266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140863205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of pest nematodes associated with soybean (<i>Glycine max</i>) in Wisconsin from 1998 to 2021.","authors":"A E MacGuidwin, D L Smith, S P Conley, K A Saikai","doi":"10.2478/jofnem-2023-0053","DOIUrl":"10.2478/jofnem-2023-0053","url":null,"abstract":"<p><p>The prevalence of <i>Heterodera glycines</i> and other cyst and vermiform genera was determined from 8,009 soil samples over two decades. Prevalence of cyst nematodes for farms increased from 16% in 1998 to 1999, reaching a peak of 40%, with marked differences among Wisconsin's nine agricultural districts in how much the odds of a positive test increased. Estimates at the sample scale also increased over time but peaked at 29%. Assay of all nematodes beginning in 2012 showed <i>Pratylenchus</i>, <i>Helicotylenchus</i>, and <i>Xiphinema</i> to be more prevalent in Wisconsin soybean fields than cyst nematodes. Prevalence estimates for <i>Pratylenchus</i> and <i>Helicotylenchus</i> for soybean and rotation crops ranged from 76 to 89% and 58 to 83%, respectively. Species identification of <i>Pratylenchus</i> from a subset of the samples revealed six species. The majority of cyst-positive samples were infested with <i>Pratylenchus</i>, and count data showed that the number of cyst eggs and juveniles per 100 cm³ soil was 60% lower in samples positive for <i>Pratylenchus</i>. The influence was reciprocal, as <i>Pratylenchus</i> population densities were 41% lower in samples positive for cyst nematodes, suggesting a competitive interaction. The Wisconsin soybean nematode testing program provides a useful model for estimating nematode prevalence using citizen-based surveys.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10981769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140338547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redetection and description of the European dagger nematode <i>Xiphinema diversicaudatum</i> on peach (<i>Prunus persica</i> L.) in Canada.","authors":"Jerry Akanwari, Qing Yu, Tahera Sultana","doi":"10.2478/jofnem-2024-0010","DOIUrl":"10.2478/jofnem-2024-0010","url":null,"abstract":"<p><p>The study reports the detection of <i>Xiphinema diversicaudatum</i> in a peach field in Ontario, Canada. Comprehensive population characterization involved morphological and molecular analyses using 18S and 28S rDNA sequences. Morphological and molecular analysis demonstrated a close relationship between the Ontario population and those from Central Europe. This is the first report of <i>X. diversicaudatum</i> from peaches (<i>Prunus persica</i>) in Canada and in North America.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10956561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140184679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Draft Transcriptome Announcement of <i>Anguina tritici</i>.","authors":"Manish Kumar, Neeraj, Alkesh Hada, Vishal Singh Somvanshi, Ashish Kumar Singh, Yashwant Kumar Yadava, Pradeep Kumar Jain, Kishore Gaikwad, Anil Sirohi","doi":"10.2478/jofnem-2024-0007","DOIUrl":"10.2478/jofnem-2024-0007","url":null,"abstract":"<p><p><i>Anguina tritici</i>, the wheat seed gall nematode, causes the 'ear-cockle' or seed gall disease of wheat (<i>Triticum sp.</i>), leading to an extensive decline of yield (30-70%) in underdeveloped wheat cultivating countries of the world. The nematode is known to survive in anhydrobiotic conditions for up to 32 years. Here, we present the first transcriptome assembly of <i>A. tritici</i>, which will be a valuable resource for understanding the genes responsible for nematode survival and above-ground plant parasitism. The final 133.2 Mb assembly consists of 105606 open reading frames (including isoforms) with the following BUSCO scores against Nematoda database: 80.3% complete (16.4% single copy and 63.9% duplicated), 2.1% fragmented, and 17.6% missing.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10954354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140175133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Depth distribution of plant-parasitic nematodes on bentgrass golf greens in Missouri and Indiana.","authors":"Asa L McCurdy, Jefferson Barizon, G L Miller","doi":"10.2478/jofnem-2024-0006","DOIUrl":"10.2478/jofnem-2024-0006","url":null,"abstract":"<p><p>Control of plant-parasitic nematodes (PPNs) on golf putting greens with nematicides is dependent on the seasonal occurrence and depth distribution of target PPN populations. This study aimed to determine if plant-parasitic nematode populations on golf course putting greens in Missouri and Indiana peaked at a targetable depth at a specific time in the year, focusing primarily on lance (<i>Hoplolaimus</i> spp.) and root-knot (<i>Meloidogyne</i> spp.) nematodes. To elucidate species diversity in the region, rDNA from a subset of lance and root-knot nematodes was sequenced and analyzed, with additional micromorphology of a lance nematode assessed in scanning electron micrographs (SEM). Soil samples were taken to a depth of 25 cm and stratified into 5 cm increments during April, June, August and October at seven sites across Missouri, three in the Kansas City metro of Kansas in 2021 and in ten sites across Indiana in 2022. Samples were stratified in five-centimeter increments and aggregated for a total of 100 cm³ of soil at each depth for each sampling. Samples were processed using a semi-automatic elutriator followed by the sucrose-flotation method, and populations were counted using a hemocytometer and recorded. For molecular characterization, rDNA was extracted and analyzed from 31 individual lance nematodes from one site in Missouri and eight sites in Indiana, and 13 root-knot nematodes from nine sites across Indiana. A significant interaction occurred between sampling month and depth for lance and ring nematodes Missouri/KS, with both PPN populations peaking at the 0-5 cm depth during October, which is well after most targeted nematicide applications are applied. Ring nematodes in Indiana did not follow this trend and were most abundant in August at a depth of 0-5 cm. No significant interaction between depth and month occurred for lance or root-knot nematodes in Indiana, or root-knot nematodes in Missouri/KS. <i>Hoplolaimus stephanus</i> and <i>H. magnistylus</i> were the lance species identified on golf greens, and <i>Meloidogyne naasi</i>, <i>M. graminicola</i> and <i>M. marylandi</i> were the root-knot species identified. Scanning-electron micrographs confirmed morphological characteristics unique to <i>H. stephanus</i>.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10954353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}