Journal of nematology最新文献

{"title":"Host Status of Ornamental Shade Trees and Shrubs to Plant Parasitic Nematodes.","authors":"T Benedetti, J E Weiland, I A Zasada","doi":"10.2478/jofnem-2024-0024","DOIUrl":"10.2478/jofnem-2024-0024","url":null,"abstract":"<p><p>Oregon leads the United States in nursery production of shade trees and is third in deciduous and broadleaf evergreen shrub production. Plant-parasitic nematodes have been implicated in problems with the growth of plants in nurseries and are also of phytosanitary risk. A greenhouse experiment was conducted to evaluate the host status of four trees (<i>Quercus alba, Quercus garryana, Acer campestre, Thuja occidentalis</i>) and two shrubs <i>(Buxus sempervirens</i>, <i>Rhododendron catawbiense</i>) to <i>Meloidogyne incognita, Meloidogyne hapla</i>, and <i>Pratylenchus neglectus</i>. Each plant/nematode treatment was replicated five times, and the experiment was conducted twice. Plants were inoculated with 3,000 eggs of <i>M. incognita</i> or <i>M. hapla</i> and 2,500 individuals of <i>P. neglectus</i> two weeks after planting. After three months, the plants were harvested, and the total density of nematodes in soil and roots for <i>P. neglectus</i> and the total density of second-stage juveniles (J2) in soil and eggs on roots for <i>M. hapla</i> and <i>M. incognita</i> were determined. The final nematode population (Pf) and reproductive factor (RF = Pf/initial population density) were calculated. For <i>M. incognita</i> and <i>M. hapla</i>, all of the ornamental trees and shrubs would be considered as fair to good hosts with RF values > 1. <i>Meloidogyne incognita</i> had the highest Pf (5,234 total J2 and eggs/pot) and RF value (28.4) on <i>A. campestre</i>. For <i>P. neglectus</i>, all of the ornamental trees and shrubs were fair to good hosts, except for <i>B. sempervirens</i>. <i>Buxus sermpervirens</i> was not a host for <i>P. neglectus,</i> with an RF value of almost 0. This is the first report of <i>Q. alba, Q. garryana</i>, and <i>A. campestre</i> as hosts for <i>M. incognita, M. hapla</i>, and <i>P. penetrans</i>. This is also the first report of <i>T. occidentalis</i> and <i>R. catawbiense</i> as hosts for <i>P. penetrans</i> and the non-host status of <i>B. sermpervirens</i> for <i>P. penetrans</i>.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240024"},"PeriodicalIF":1.4,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11323970/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141982559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introduction to <i>Strongyloides stercoralis</i> Anatomy.","authors":"Michelle L Castelletto, Damia Akimori, Ruhi Patel, Nathan E Schroeder, Elissa A Hallem","doi":"10.2478/jofnem-2024-0019","DOIUrl":"10.2478/jofnem-2024-0019","url":null,"abstract":"<p><p><i>Strongyloides stercoralis</i>, commonly known as the human threadworm, is a skin-penetrating gastrointestinal parasitic nematode that infects hundreds of millions of people worldwide. Like other <i>Strongyloides</i> species, <i>S. stercoralis</i> is capable of cycling through a single free-living generation. Although <i>S. stercoralis</i> and the free-living nematode <i>Caenorhabditis elegans</i> are evolutionarily distant, the free-living adults of <i>S. stercoralis</i> are similar enough in size and morphology to <i>C. elegans</i> adults that techniques for generating transgenics and knockouts in <i>C. elegans</i> have been successfully adapted for use in <i>S. stercoralis</i>. High-quality genomic and transcriptomic data are also available for <i>S. stercoralis</i>. Thus, one can use a burgeoning array of functional genomic tools in <i>S. stercoralis</i> to probe questions about parasitic nematode development, physiology, and behavior. Knowledge gained from <i>S. stercoralis</i> will inform studies of other parasitic nematodes such as hookworms that are not yet amenable to genetic manipulation. This review describes the basic anatomy of <i>S. stercoralis</i>.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240019"},"PeriodicalIF":1.4,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11162604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report of Mermithidae (Enoplea: Mermithida) Parasitizing Adult Stable Flies in Japan.","authors":"Kaori Shimizu, Taizo Saito, Yasuhiro Takashima, Haruhiko Okada, Mitsuhiko Asakawa, Yasuo Inoshima","doi":"10.2478/jofnem-2024-0022","DOIUrl":"10.2478/jofnem-2024-0022","url":null,"abstract":"<p><p>Mermithidae is a family of nematodes that parasitize a wide range of invertebrates worldwide. Herein, we report nematodes that were unexpectedly found in three of 486 adult stable flies (<i>Stomoxys calcitrans</i>) captured from three farms (F1, F2, and F3) in different regions of Gifu Prefecture, Japan. We aimed to characterize these nematodes both at the morphological and molecular level. Morphological studies revealed that the nematodes were juveniles of Mermithidae. Phylogenetic analysis based on 18S and 28S rDNA indicated that the mermithids from farms F1 and F2 could be categorized into the same cluster as <i>Ovomermis sinensis</i> and <i>Hexamermis</i> sp., whereas the mermithid from farm F3 clustered with <i>Amphimermis</i> sp. Additionally, these mermithids could be categorized within the same clusters as related mermithids detected in Japan that parasitize various arthropod orders. Our findings suggest that these stable flies may have been parasitized by mermithids already present in the region and that genetically distinct species of mermithids occur across Japan. To the best of our knowledge, this is the first report of mermithids parasitizing adult stable flies in Japan.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240022"},"PeriodicalIF":1.4,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141283942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the use of metabarcoding to reveal eukaryotic associations with mononchids nematodes.","authors":"Joseph O Maosa, Siqi Wang, Shuhan Liu, Hongmei Li, Xue Qing, Wim Bert","doi":"10.2478/jofnem-2024-0016","DOIUrl":"10.2478/jofnem-2024-0016","url":null,"abstract":"<p><p>Nematodes play a vital ecological role in soil and marine ecosystems, but there is limited information about their dietary diversity and feeding habits. Due to methodological challenges, the available information is based on inference rather than confirmed observations. The lack of correct dietary requirements also hampers rearing experiments. To achieve insight into the prey of mononchid nematodes, this study employed high-throughput Illumina paired-end sequencing using universal eukaryotic species 18S primers on 10 pooled mononchid nematode species, namely <i>Mylonchulus brachyuris, M. brevicaudatus, Mylonchulus</i> sp., <i>Clarkus parvus, Prionchulus</i> sp. <i>M. hawaiiensis, M. sigmaturellus, M. vulvapapillatus, Anatonchus</i> sp. and <i>Miconchus</i> sp. The results indicate that mononchids are associated with a remarkable diversity of eukaryotes, including fungi, algae, and protists. While the metabarcoding approach, first introduced here for mononchids, proved to be a simple and rapid method, it has several limitations and crucial methodological challenges that should be addressed in future studies. Ultimately, such methods should be able to evaluate the dietary complexity of nematodes and provide a valuable avenue for unraveling the dietary requirements of previously unculturable nematodes. This can contribute to the methodology of understanding their feeding habits and contributions to ecosystem dynamics.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240016"},"PeriodicalIF":1.4,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11086744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140912485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant-parasitic Nematodes Associated with Grasses Grown for Seed in the Willamette Valley of Oregon.","authors":"H M Rivedal, I A Zasada, T N Temple, A B Peetz, L A Núñez-Rodríguez, R J Starchvick, E T Braithwaite","doi":"10.2478/jofnem-2024-0020","DOIUrl":"10.2478/jofnem-2024-0020","url":null,"abstract":"<p><p>Plant-parasitic nematodes (PPN) are an understudied pathogen group in the Oregon cool-season grass seed cropping system. In this survey, the PPN associated with annual ryegrass, bentgrass, fine fescue, orchardgrass, perennial ryegrass, and tall fescue were determined. Thirty-seven fields were sampled in the 2022 or 2023 growing season by collecting 10 soil cores in each of six 100-m transects for nematode extraction and visual identification. PerMANOVA testing indicated significant differences in PPN community composition across grass host and sampling time. <i>Pratylenchus</i> and <i>Meloidogyne</i> were the most commonly encountered nematodes, with maximum population densities of 1,984 and 2,496 nematodes/100 g soil, respectively. Sequencing of the COX1 gene region indicated the presence of <i>P. crenatus, P. fallax, P. neglectus, P. penetrans</i>, and <i>P. thornei</i>, with some of these species being detected for the first time on these grass hosts. The only <i>Meloidogyne</i> sp. found in these grasses was <i>M. nassi</i>, based upon sequencing of the ITS gene region. This first-of-its-kind survey indicates the need for further assessment of the impact of these PPNs on yield and stand longevity in cool-season grass seed fields in Oregon.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240020"},"PeriodicalIF":1.4,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11086743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140913707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Efficacy of Entomopathogenic Nematodes Plus an Adjuvant against <i>Helicoverpa zea</i> and <i>Chrysodeixis includens</i> in Aboveground Applications.","authors":"Minling Zhang, Nathan Spaulding, Gadi V P Reddy, David I Shapiro-Ilan","doi":"10.2478/jofnem-2024-0018","DOIUrl":"10.2478/jofnem-2024-0018","url":null,"abstract":"<p><p>In the southern United States, corn earworm, <i>Helicoverpa zea</i> (Boddie), and soybean looper, <i>Chrysodeixis includens</i> (Walker) are economically important crop pests. Although Bt crops initially provided effective control of target pests such as <i>H. zea</i>, many insect pests have developed resistance to these Bt crops. Alternative approaches are needed, including biological control agents such as entomopathogenic nematodes (EPNs). However, the effectiveness of EPNs for aboveground applications can be limited due to issues such as desiccation and ultraviolet radiation. Effective adjuvants are needed to overcome these problems. Ten strains of EPNs were tested for virulence against eggs, first to fourth instars, fifth instars, and pupae of <i>H. zea</i> and <i>C. includens</i> in the laboratory. These 10 EPN strains were <i>Heterorhabditis bacteriophora</i> (HP88 and VS strains), <i>H. floridensis</i> (K22 strain), Hgkesha (Kesha strain), <i>Steinernema carpocapsae</i> (All and Cxrd strains), <i>S. feltiae</i> (SN strain), <i>S. rarum</i> (17c+e strain), and <i>S. riobrave</i> (355 and 7-12 strains). EPNs could infect eggs of <i>H. zea</i> or <i>C. includens</i> in the laboratory, but the infection was low. The mortality caused by 10 EPN strains in seven days was significantly higher for the first to fourth instars of <i>H. zea</i> compared to the control, as was the fifth instars of <i>H. zea</i>. Similarly, for the first to fourth and fifth instars of <i>C. includens</i>, the mortality was significantly higher compared to the controls, respectively. However, only <i>S. riobrave</i> (355) had significantly higher mortality than the control for the pupae of <i>H. zea</i>. For the pupae of <i>C. includens</i>, except for <i>H. bacteriophora (HP88)</i>, <i>S. rarum (17c+e)</i>, and <i>H. floridensis</i> (K22), the mortality of the other seven strains was significantly higher than the control. Subsequently, <i>S. carpocapsae</i> (All) and <i>S. riobrave</i> (7-12) were chosen for efficacy testing in the field with an adjuvant 0.066% Southern Ag Surfactant (SAg Surfactant). In field experiments, the SAg Surfactant treatment significantly increased the mortality and EPN infection for <i>S. carpocapsae</i> (All) on first instars of <i>H. zea</i> in corn plant whorls. On soybean plants, with the SAg Surfactant, <i>S. carpocapsae</i> (All) was more effective than <i>S. riobrave</i> (7-12) on fifth instars of <i>C. includens</i>. This study indicates that EPNs can control <i>H. zea</i> and <i>C. includens</i>, and SAg Surfactant can enhance EPN efficacy.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240018"},"PeriodicalIF":1.4,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11078269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140891913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geographical distribution and phoretic associations of the viviparous nematode <i>Tokorhabditis atripennis</i> with <i>Onthophagus</i> dung beetles in Japan.","authors":"Yuya Ikeda, Yuto Koike, Ryoji Shinya, Koichi Hasegawa","doi":"10.2478/jofnem-2024-0013","DOIUrl":"10.2478/jofnem-2024-0013","url":null,"abstract":"<p><p>Viviparity is generally considered to be rare in animals. In nematodes, only six species of Rhabditida are viviparous. Five of these species have been identified in association with <i>Onthophagus</i> dung beetles, with <i>Tokorhabditis atripennis</i> being repeatedly isolated from the dung beetle <i>Onthophagus atripennis</i> in Japan. <i>T. atripennis</i> is easy to culture in a laboratory setting, and its host, <i>O. atripennis</i>, is distributed all over Japan. Therefore, <i>T. atripennis</i> is an ideal candidate for ecological and evolutionary studies on viviparity. However, the extent of their distribution and relationship with dung beetles, as well as habitats, remain unclear. In the present study, we conducted field surveys and successfully isolated 27 strains of viviparous nematodes associated with tunneler dung beetles from various regions of Japan, all of which were identified as <i>T. atripennis</i>. <i>T. atripennis</i> exhibited a strong association with <i>Onthophagus</i> dung beetles, especially <i>O. apicetinctus</i> and <i>O. atripennis</i>. And it was predominantly found in specific anatomical locations on the beetle bodies, such as the 'groove between pronotum and elytron' and the 'back of the wings'. Our findings suggest that <i>Onthophagus</i> species are the primary hosts for <i>T. atripennis</i>, and <i>T. atripennis</i> exhibits a close relationship with the living environments of tunneler beetles. This association may play a significant role in the evolution of viviparity in nematodes.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240013"},"PeriodicalIF":1.4,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11044808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140855300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vertical Migration of Second-stage Juveniles of <i>Meloidogyne enterolobii</i> as Influenced by Temperature and Host.","authors":"Ana Karina S Oliveira, Elvira M R Pedrosa, Diego A H S Leitão, Janete A Brito, Ênio F de F Silva, Donald W Dickson","doi":"10.2478/jofnem-2024-0012","DOIUrl":"10.2478/jofnem-2024-0012","url":null,"abstract":"<p><p>Infective second-stage juveniles (J2) of <i>Meloidogyne</i> spp. migrate towards host roots, which depends on several factors, including root exudates and soil temperature. Although <i>Meloidogyne enterolobii</i> is a highly virulent nematode that affects major agricultural crops worldwide, there is limited ecological data about it. The objective of this study was to determine the J2 migration pattern vertically in 14-cm long segmented soil columns towards tomato (<i>Solanum lycopersicum</i>) and marigold (<i>Tagetes patula</i>) roots, each grown at two soil temperatures (20 or 26ºC). Bottomless cups with tomatoes or marigolds were attached to the top of each column; cups with no plants were used as untreated controls. Juveniles (1,000/column) were injected into a hole located 1 cm from the bottom of each column. The apparatuses were placed in growth chambers at 20 or 26ºC, and J2 were allowed to migrate for 3, 6, 9, or 12 days after injection (DAI). At each harvest, J2 were extracted from each ring of the columns and counted to compare their distribution, and root systems were stained to observe root penetration. <i>M. enterolobii</i> migrated over 13 cm vertically 3 DAI regardless of temperature, even without plant stimuli. The vertical migration was greater at 26ºC, where 60% of active J2 were found at distances >13 cm at 12 DAI. Temperature did not affect root penetration. Overall, a greater number of J2 was observed in tomato roots, and root penetration increased over time.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240012"},"PeriodicalIF":1.4,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11033720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140865005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cotton host resistance as a tool for managing <i>Rotylenchulus reniformis</i> in Louisiana.","authors":"Tristan T Watson","doi":"10.2478/jofnem-2024-0014","DOIUrl":"10.2478/jofnem-2024-0014","url":null,"abstract":"<p><p>The reniform nematode, <i>Rotylenchulus reniformis</i>, is a major yield-limiting pest of upland cotton (<i>Gossypium hirsutum</i>) in the United States that has been steadily increasing in incidence in many states. Reniform nematode-resistant cotton cultivars have recently become commercially available for cotton producers; however, few field trials have evaluated their efficacy as a nematode management tool. The aim of this study was to evaluate reniform nematode population development, plant growth, and seed cotton yield of reniform nematode-resistant cotton cultivars in two nematode-infested fields in Louisiana. Replicated small-plot field trials were conducted in St. Joseph, LA (NERS field) and Winnsboro, LA (MRRS field) during the 2022 and 2023 growing seasons. In 2022, cultivars evaluated included: (1) DP 1646 B2XF (susceptible/tolerant), (2) DP 2141NR B3XF (resistant), (3) PHY 332 W3FE (resistant), (4) PHY 411 W3FE (resistant), and (5) PHY 443 W3FE (resistant). In 2023, an additional susceptible cotton cultivar, PHY 340 W3FE, was also included. All nematode-resistant cotton cultivars evaluated provided suppression of reniform nematode population development relative to that of the susceptible cotton cultivars, with suppression of nematode soil population densities at harvest ranging from 49 - 81% relative to DP 1646 B2XF. The resistant cultivar PHY 411 W3FE provided the most consistent suppression of reniform nematode population development, reducing reniform nematode soil population densities at harvest in both field locations and both trial years. In contrast, DP 2141NR B3XF only reduced soil population densities at harvest in the NERS field in 2023. Despite relatively consistent nematode suppression and improvements in plant vigor ratings and canopy coverage associated with the resistant cotton cultivars, a yield increase was only observed with PHY 332 W3FE and PHY 411 W3FE planted at the NERS field in 2023. Despite strong resistance to reniform nematode in the evaluated cotton cultivars, nematode soil population densities still increased during the growing season in plots planted with resistant cotton cultivars, emphasizing the need for additional management tactics to use alongside host resistance. This study indicates that new reniform nematode-resistant cotton cultivars show promising potential to reduce nematode population development during the growing season in Louisiana.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240014"},"PeriodicalIF":1.4,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11033718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140855236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of <i>Nothotylenchus savadkoohensis</i> n. sp. (Rhabditida, Anguinidae) from Iran based on morphological and molecular data.","authors":"Soheila Rezaei, Ebrahim Pourjam, Mohammad Reza Atighi, Majid Pedram","doi":"10.2478/jofnem-2024-0015","DOIUrl":"10.2478/jofnem-2024-0015","url":null,"abstract":"<p><p><i>Nothotylenchus savadkoohensis</i> n. sp. was recovered from rotten wood samples of an unidentified forest tree in the Mazandaran province and described herein. It is mainly characterized by an elongated conoid tail ending in a sharply pointed tip and four lines in the lateral field. Females of the new species have 379-662 μm long bodies with 5.8-6.9 μm long stylets ending in fine posteriorly sloping knobs, the metacorpus not valvate, the pharyngeal bulb slightly overlapping the intestine, and the vulva at 76.5-84.0% of body length. Males are also common and have 13.0-14.5 μm long spicules and bursa cloacal. By having an elongated conoid tail and four lines in the lateral field, the new species comes close to four known species, namely <i>N. acris, N. acutus, N. antricolus</i>, and <i>N. truncatus</i>. The morphological differences between the new species and the abovementioned species are discussed. The new species was sequenced for its D2-D3 segment of LSU and ITS rDNA regions. In the LSU phylogenetic tree, the currently available LSU sequences of the genus <i>Nothotylenchus</i> occupied distant placements from each other and the LSU sequence of the new species formed clade with a sequence assigned to <i>Neotylenchus</i> sp. In ITS phylogeny, the newly generated sequence of the new species formed a clade with a clade that includes sequences of <i>Ditylenchus</i> sp. and <i>Neomisticius platypi</i> and <i>N. variabilis</i>.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"56 1","pages":"20240015"},"PeriodicalIF":1.4,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11033712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}