Journal of nematology最新文献_第10页

Morphological and Molecular Characterization of Filenchus multistriatus n. sp. (Tylenchomorpha: Tylenchidae) and Data on a Known Species of the Genus from Bushehr Province, Southern Iran. 伊朗南部布什尔省Filenchus multistatus n.sp.的形态和分子特征（Tylenchomorpha：Tylenchidae）以及该属一已知物种的数据。

IF 1.3 4区生物学

Journal of nematology Pub Date : 2023-05-17 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0008

Somayeh Monemi, Mohammad Reza Atighi, Joaquín Abolafia, Pablo Castillo, Majid Pedram

{"title":"Morphological and Molecular Characterization of Filenchus multistriatus n. sp. (Tylenchomorpha: Tylenchidae) and Data on a Known Species of the Genus from Bushehr Province, Southern Iran.","authors":"Somayeh Monemi, Mohammad Reza Atighi, Joaquín Abolafia, Pablo Castillo, Majid Pedram","doi":"10.2478/jofnem-2023-0008","DOIUrl":"10.2478/jofnem-2023-0008","url":null,"abstract":"During a nematological survey in southern Iran, a population belonging to the family Tylenchidae was recovered from a tomato field in Bushehr province. The recovered population belongs to the genus Filenchus, was described and illustrated herein as F. multistriatus n. sp. It is mainly characterized by having a wide and low annulated lip region continuous with adjacent body; amphidial openings confined to the labial plate; four lines in lateral fields forming three bands, with the two outer bands broken by transverse, and the inner one broken by both transverse and longitudinal lines; and median bulb oval with visible valve and elongate-conoid tail uniformly and gradually narrowing toward the distal region, ending in a widely rounded tip. Its morphological and morphometric differences with three closely similar species were discussed. The phylogenetic relationships of the new species with other relevant genera and species were reconstructed using partial sequences of small, and large subunit ribosomal DNA (SSU and LSU rDNA) sequences. Morphometric and morphological data were also provided for an Iranian population of F. sandneri recovered from Bushehr province. Both populations were characterised using SEM data.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230008"},"PeriodicalIF":1.3,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10191216/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10234280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of Plant-Parasitic Nematodes in Michigan Corn Fields. 密歇根玉米地植物寄生线虫的分布。

IF 1.4 4区生物学

Journal of nematology Pub Date : 2023-05-02 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0015

Sita Thapa, Elisabeth Darling, Emilie Cole, Kristin Poley, Marisol Quintanilla

{"title":"Distribution of Plant-Parasitic Nematodes in Michigan Corn Fields.","authors":"Sita Thapa, Elisabeth Darling, Emilie Cole, Kristin Poley, Marisol Quintanilla","doi":"10.2478/jofnem-2023-0015","DOIUrl":"10.2478/jofnem-2023-0015","url":null,"abstract":"Plant-parasitic nematodes (PPNs) can cause substantial economic yield losses to many agronomic crops in the United States. A regional-scale survey was completed across 20 counties to determine PPNs prevalence in Michigan corn and how factors such as soil type, tillage, irrigation, and cropping systems influence their distribution. Ten different major genera of PPNs were identified in Michigan corn fields: Longidorus (needle), Helicotylenchus (spiral), Pratylenchus (lesion), Meloidogyne (root-knot), Heterodera (cyst), Hoplolaimus (lance), Tylenchorhynchus or Merlinius (stunt), Paratylenchus (pin), Criconemella (ring), and Xiphinema (dagger). No significant differences among different categories of tillage for lesion, stunt, or needle nematode prevalence was detected. Lesion nematodes were most prevalent in muck soil, while stunt nematode prevalence was significantly affected by the soil type. Needle nematodes were least abundant in irrigated soils and in contrast, stunt nematodes were higher in non-irrigated soils. Spiral nematodes were the most common PPNs in Michigan corn in all cropping systems. These findings will be helpful in planning future nematode studies in Michigan and in developing and evaluating corn nematode management strategies.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230015"},"PeriodicalIF":1.4,"publicationDate":"2023-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10353600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10291495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reaction of Winter Cover Crops to Meloidogyne enterolobii and Glasshouse Bioassay for Evaluating Utility in Managing M. enterolobii in Soybeans. 冬覆盖作物对肠根结线虫的反应和用于评估大豆中肠根结霉菌管理效用的玻璃屋生物测定。

IF 1.4 4区生物学

Journal of nematology Pub Date : 2023-05-02 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0014

Neel Saha, Tanner Schwarz, Samantha Mowery, Adrienne M Gorny

{"title":"Reaction of Winter Cover Crops to Meloidogyne enterolobii and Glasshouse Bioassay for Evaluating Utility in Managing M. enterolobii in Soybeans.","authors":"Neel Saha, Tanner Schwarz, Samantha Mowery, Adrienne M Gorny","doi":"10.2478/jofnem-2023-0014","DOIUrl":"10.2478/jofnem-2023-0014","url":null,"abstract":"Meloidogyne enterolobii is an invasive and highly aggressive root-knot nematode pathogen impacting the Southeastern United States. Winter cover cropping may be a cost-effective method for reducing populations of M. enterolobii in between summer cash crops, yet a gap in the knowledge remains about the response of these cover crops to M. enterolobii and their utility in suppressing nematode populations prior to a cash crop. A \"two-step\" glasshouse bioassay was performed to evaluate eight winter cover crops popular in North Carolina for their direct response to M. enterolobii infection, and to quantify their effect in reducing nematode populations for the following soybean plants. Data on cover crop root galling, soybean root galling, soybean shoot fresh weight, soybean root fresh weight, eggs per gram of soybean root, and a modified reproductive factor were collected. Cereal cover crops did not display root galling, and there was significantly less root galling in those soybean plants following cereal winter cover crops when compared to those following broadleaf winter cover crops. Broadleaf winter cover crops resulted in significantly higher eggs per gram of soybean root and modified reproductive factor in the soybean plants, compared to cereal cover crops and non-inoculated controls. Results from this study suggest that cereal winter cover crops may be poor-hosts to M. enterolobii and may significantly reduce M. enterolobii populations before a soybean crop, compared to broadleaf winter cover crops. This study lays the groundwork for management recommendations and future field trials to assess management of M. enterolobii through winter cover cropping.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230014"},"PeriodicalIF":1.4,"publicationDate":"2023-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10236376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10236399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

18S-NemaBase: Curated 18S rRNA Database of Nematode Sequences. 18S NemaBase：线虫序列的18S rRNA Curated数据库。

IF 1.4 4区生物学

Journal of nematology Pub Date : 2023-04-21 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0006

Kaitlin Gattoni, Eli M S Gendron, Rebeca Sandoval-Ruiz, Abigail Borgemeier, J Parr McQueen, Rachel M Shepherd, Dieter Slos, Thomas O Powers, Dorota L Porazinska

{"title":"18S-NemaBase: Curated 18S rRNA Database of Nematode Sequences.","authors":"Kaitlin Gattoni, Eli M S Gendron, Rebeca Sandoval-Ruiz, Abigail Borgemeier, J Parr McQueen, Rachel M Shepherd, Dieter Slos, Thomas O Powers, Dorota L Porazinska","doi":"10.2478/jofnem-2023-0006","DOIUrl":"10.2478/jofnem-2023-0006","url":null,"abstract":"Nematodes are the most abundant and diverse animals on the planet but lack representation in biodiversity research. This presents a problem for studying nematode diversity, particularly when molecular tools (i.e., barcoding and metabarcoding) rely on well-populated and curated reference databases, which are absent for nematodes. To improve molecular identification and the assessment of nematode diversity, we created and curated an 18S rRNA database specific to nematodes (18S-NemaBase) using sequences sourced from the most recent publicly available 18S rRNA SILVA v138 database. As part of the curation process, taxonomic strings were standardized to contain a fixed number of taxonomic ranks relevant to nematology and updated for the most recent accepted nematode classifications. In addition, apparent erroneous sequences were removed. To test the efficacy and accuracy of 18S-NemaBase, we compared it to an older but also curated SILVA v111 and the newest SILVA v138 by assigning taxonomies and analyzing the diversity of a nematode dataset from the Western Nebraska Sandhills. We showed that 18S-NemaBase provided more accurate taxonomic assignments and diversity assessments than either version of SILVA, with a much easier workflow and no need for manual corrections. Additionally, observed diversity further improved when 18S-NemaBase was supplemented with reference sequences from nematodes present in the study site. Although the 18S-NemaBase is a step in the right direction, a concerted effort to increase the number of high-quality, accessible, full-length nematode reference sequences is more important now than ever.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230006"},"PeriodicalIF":1.4,"publicationDate":"2023-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10152464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10601724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Report of the Gastropod-Killing Nematode, Phasmarhabditis californica, in Washington State, U.S.A. 美国华盛顿州加利福尼亚Phasmarhabditis腹足线虫病的首次报告。

IF 1.3 4区生物学

Journal of nematology Pub Date : 2023-04-21 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0013

Rory J Mc Donnell, Dana K Howe, Dee R Denver

引用次数: 0

Morphological and Molecular Characterization of Ottolenchus isfahanicus n. sp. (Tylenchomorpha: Tylenchoidea) from Rhizosphere of Gramineous Plants in Isfahan Province, Iran. 伊朗伊斯法罕省禾本科植物根际Ottolenchus isfahanicus n.sp.（Tylenchomorpha:Tylenchoidea）的形态和分子特征。

IF 1.3 4区生物学

Journal of nematology Pub Date : 2023-04-21 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0011

Zeinab Mahboubi, Mojtaba Keramat, Ebrahim Pourjam, Mohammad Reza Atighi, Ilenia Clavero-Camacho, Joaquín Abolafia, Pablo Castillo, Majid Pedram

{"title":"Morphological and Molecular Characterization of Ottolenchus isfahanicus n. sp. (Tylenchomorpha: Tylenchoidea) from Rhizosphere of Gramineous Plants in Isfahan Province, Iran.","authors":"Zeinab Mahboubi, Mojtaba Keramat, Ebrahim Pourjam, Mohammad Reza Atighi, Ilenia Clavero-Camacho, Joaquín Abolafia, Pablo Castillo, Majid Pedram","doi":"10.2478/jofnem-2023-0011","DOIUrl":"10.2478/jofnem-2023-0011","url":null,"abstract":"A new species from the family Tylenchidae was recovered in the grasslands of Isfahan province, Iran, and is herein described based on morphological and molecular data. Ottolenchus isfahanicus n. sp. is mainly characterized by having a faintly annulated cuticle; elongated, slightly sigmoid amphidial apertures developed metacorpus with distinct valve under LM; vulva located at 69.472.3% of body length; large spermatheca about 2.75 times of corresponding body width; and elongated conoid tail with broadly rounded tip. SEM observations showed that the lip region is smooth; the amphidial apertures are elongated, slightly sigmoid slits; and the lateral field is a simple band. It is further characterized by 477-to-515-μm-long females with delicate 5.7-to-6.9-μm-long stylets with small, slightly posteriorly sloping knobs, as well as functional males, in the population. The new species closely resembles O. facultativus, but is separated from it based on morphological and molecular data. It was further morphologically compared with O. discrepans, O. fungivorus, and O. sinipersici. The phylogenetic relationships of the new species with other relevant genera and species were reconstructed using near-full-length sequences of small subunit and D2-D3 expansion segments of large subunit (SSU and LSU D2-D3). In the inferred SSU phylogeny, the newly generated sequence of Ottolenchus isfahanicus n. sp. formed a clade with two sequences of O. sinipersici and sequences assigned to O. facultativus and O. fungivorus. In the inferred LSU phylogeny, the three newly generated sequences of the new species and LSU sequences of O. sinipersici and O. discrepans formed a clade.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230011"},"PeriodicalIF":1.3,"publicationDate":"2023-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10152461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10601725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Planting Date, Cultivar and Vernalization Using Gibberellic Acid on the Severity of Root-Knot Nematode Damage to Globe Artichoke in Subtropical Sandy Soil. 在亚热带沙质土壤中，种植日期、品种和赤霉素春化对朝鲜蓟根结线虫危害程度的影响。

IF 1.4 4区生物学

Journal of nematology Pub Date : 2023-04-19 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0012

Hung X Bui, Shinsuke Agehara, Weining Wang, Johan A Desaeger

{"title":"Effects of Planting Date, Cultivar and Vernalization Using Gibberellic Acid on the Severity of Root-Knot Nematode Damage to Globe Artichoke in Subtropical Sandy Soil.","authors":"Hung X Bui, Shinsuke Agehara, Weining Wang, Johan A Desaeger","doi":"10.2478/jofnem-2023-0012","DOIUrl":"10.2478/jofnem-2023-0012","url":null,"abstract":"Globe artichoke (Cynara cardunculus var. scolymus L.) is a new alternative crop in Florida. This long-season crop poses a very high risk of root-knot nematode (RKN) damage, the most important nematode problem in vegetable production in Florida. This study aimed to examine the impact of RKN damage on artichoke production in the subtropical climate of Florida. Treatments consisted of four cultivars ('Green Globe Improved', 'Green Queen', 'Imperial Star', and 'Opal') planted on three different dates (October 5, October 19, and November 2) in Experiment 1, and three cultivars ('Green Globe Improved', 'Green Queen', and 'Imperial Star') grown with or without vernalization using gibberellic acid (GA3) in Experiment 2. Both field experiments were conducted on sandy soil in west-central Florida during the 2020-2021 and 2021-2022 growing seasons. We collected RKN population density and gall index (GI) data to assess RKN damage. In Experiment 1, all tested cultivars showed moderate to high RKN infection in the 2021-2022 season, with 43% to 75% of roots galled. There was no effect of planting dates on RKN damage in the 2020-2021 season. However, delaying the planting date from October 5 to November 2 reduced the RKN damage while increasing the RKN population densities in the 2021-2022 season. In Experiment 2, all tested cultivars showed high RKN infestation, with more than 80% of roots galled. Vernalization by GA3 did not affect the severity of RKN damage. Our results suggest that all tested artichoke cultivars are highly susceptible to RKN in subtropical environments of Florida, raising an alarm on the risk of RKN damage to commercial artichoke production and increasing awareness about the need for RKN management.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230012"},"PeriodicalIF":1.4,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10152460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10601723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meloidogyne paramali n. sp. (Nematoda: Meloidogyninae) and First Report of M. marylandi in maple and yacca tree from Japan. 日本槭树和桉树的马利氏线虫(线虫纲:马利氏线虫科)和马利氏线虫首次报告。

IF 1.4 4区生物学

Journal of nematology Pub Date : 2023-04-19 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2022-0036

Jianfeng Gu, Yiwu Fang, Xinxin Ma, Baolin Shao, Kan Zhuo

{"title":"Meloidogyne paramali n. sp. (Nematoda: Meloidogyninae) and First Report of M. marylandi in maple and yacca tree from Japan.","authors":"Jianfeng Gu, Yiwu Fang, Xinxin Ma, Baolin Shao, Kan Zhuo","doi":"10.2478/jofnem-2022-0036","DOIUrl":"10.2478/jofnem-2022-0036","url":null,"abstract":"Meloidogyne paramali n. sp. was detected from Japanese maple trees (Acer palmatum) from Chiba, Japan during quarantine inspections in China. This species is characterized by second-stage juveniles (J2) with short tail length 32.2 (24-36.8) μm, finely rounded to broadly pointed tail terminus with extremely short hyaline tail terminus 4.3 (3.0-4.9) μm; perineal patterns of females characterized by an oval or irregular appearance, with round and low dorsal arch, and fine and smooth striae. M. paramali n. sp. is very similar to M. mali in that the perineal pattern has fine, smooth striae and both J2 have a short tail, but it can be distinguished from the latter by perineal pattern of the female (lateral field distinct vs. indistinct), shorter J2 hyaline tail terminus (4.3 [3.0-4.9] μm vs. 8.2 [4.8-12.7] μm, and by J2 tail with finely rounded to broadly pointed tail terminus, never sharply pointed vs. finely rounded and almost pointed. The polytomous key codes of the new species are as follows: Female: A21, B2, C32, D4; Male: A21, B3, C2, D1, E2, F2; J2: A2, B23, C43, D34, E12, F34. Detailed phylogenetic analysis based on partial 18S, ITS, D2-D3 28S, and partial mtCOI sequences also confirmed it as a new species, which is very close to M. mali and M. vitis and forms molecular group VIII. M. marylandi and other Meloidogyne species detected from plants from Japan in China are also discussed.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20220036"},"PeriodicalIF":1.4,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10152465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10488867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant-Parasitic Nematodes and their Effects on Ornamental Plants: A Review. 植物寄生线虫及其对观赏植物的影响：综述。

IF 1.4 4区生物学

Journal of nematology Pub Date : 2023-04-14 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0007

Amanda D Howland, Marisol Quintanilla

{"title":"Plant-Parasitic Nematodes and their Effects on Ornamental Plants: A Review.","authors":"Amanda D Howland, Marisol Quintanilla","doi":"10.2478/jofnem-2023-0007","DOIUrl":"10.2478/jofnem-2023-0007","url":null,"abstract":"Worldwide, the ornamental plant industry is estimated to be valued at $70 billion, with the United States' ornamental plant industry valued at $4.8 billion in 2020. Ornamental plants are cultivated for numerous reasons worldwide, such as decorative, medicinal, social, and utility purposes, making the ornamental field a high growth industry. One of the main pathogen groups affecting the yield and growth of the ornamental plant industry is plant-parasitic nematodes, which are microscopic roundworms that feed on plant parts causing significant yield loss. There are many kinds of plant-parasitic nematodes that affect ornamental plants, with the main genera being Meloidogyne spp., Aphelenchoides spp., Paratylenchus spp., Pratylenchus spp., Helicotylenchus spp., Radopholus spp., Xiphinema spp., Trichodorus spp., Paratrichodorus spp., Rotylenchulus spp., and Longidorus spp. The aim of this review is to focus on the effects, hosts, and symptoms of these major plant-parasitic nematodes on ornamental plants and synthesize current management strategies in the ornamental plant industry.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230007"},"PeriodicalIF":1.4,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10111207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10237748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Vicia sativa L. on Motility, Mortality and Expression Levels of hsp Genes in J2 Stage of Meloidogyne hapla. Vicia sativa L.对根结线虫J2期活力、死亡率及hsp基因表达水平的影响。

IF 1.4 4区生物学

Journal of nematology Pub Date : 2023-04-14 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0009

Renata Dobosz, Łukasz Flis, Jan Bocianowski, Tadeusz Malewski

{"title":"Effect of Vicia sativa L. on Motility, Mortality and Expression Levels of hsp Genes in J2 Stage of Meloidogyne hapla.","authors":"Renata Dobosz, Łukasz Flis, Jan Bocianowski, Tadeusz Malewski","doi":"10.2478/jofnem-2023-0009","DOIUrl":"10.2478/jofnem-2023-0009","url":null,"abstract":"Assuming that the seeds of Vicia sativa L. have a stressful effect on J2 stage Meloidogyne hapla, we undertook research on the effect of these seeds on the motility and mortality of J2 and determined the expression levels of selected hsp genes in J2. The assessment of the effect of V. sativa seeds on the motility of M. hapla specimens consisted of observing the movement of J2 immersed in a seed diffusate or in a tomato root filtrate at temperatures of 10, 17, and 21°C. In J2 treated with V. sativa (cv. Ina) seed diffusates, the expression level of hsp genes was determined by qPCR. J2 exposed to V. sativa diffusates were found to lose their motility, while their mortality did not exceed 30%. J2 in the seed diffusate were characterized by an increase in the expression levels of the Mh-hsp90, Mh-hsp1, and Mh-hsp43 genes. It is suggested that the hsp90 gene may be a potential bioindicator of the environmental impact on Meloidogyne nematodes. The impaired ability to move in J2 of M. hapla is attributable to the occurrence of V. sativa seeds in their habitat. These studies may contribute to developing methods of reducing crop damage caused by M. hapla.","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230009"},"PeriodicalIF":1.4,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10111211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10237754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0