Journal of nematology最新文献

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Virulence of Two Isolates of Meloidogyne enterolobii (Guava Root-Knot Nematode) from North Carolina on Cotton Lines Resistant to Southern Root-Knot Nematode (M. incognita) and Reniform Nematode (Rotylenchulus reniformis). 来自北卡罗来纳州的两个瓜根结线虫分离株对棉花抗南方根结线虫(M.incognita)和肾形线虫(Rotylenchulus reniformis)的毒力。
IF 1.3 4区 生物学
Journal of nematology Pub Date : 2023-06-23 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0021
Amanda G Gaudin, Martin J Wubben, Jack C McCarty, Johnie N Jenkins
{"title":"Virulence of Two Isolates of <i>Meloidogyne enterolobii</i> (Guava Root-Knot Nematode) from North Carolina on Cotton Lines Resistant to Southern Root-Knot Nematode (<i>M. incognita</i>) and Reniform Nematode (<i>Rotylenchulus reniformis</i>).","authors":"Amanda G Gaudin, Martin J Wubben, Jack C McCarty, Johnie N Jenkins","doi":"10.2478/jofnem-2023-0021","DOIUrl":"10.2478/jofnem-2023-0021","url":null,"abstract":"<p><p><i>Meloidogyne enterolobii</i> [the guava root-knot nematode (RKN)] is an emerging plant-parasitic nematode that poses a threat to Upland cotton (<i>Gossypium hirsutum</i>) production in the southeastern United States. Like other RKN spp., <i>M. enterolobii</i> has a wide host range and proven ability to overcome resistance sources that have helped protect crops from other <i>Meloidogyne</i> spp., including the southern RKN (<i>Meloidogyne incognita</i>). In this study we evaluated the virulence of two North Carolina <i>M. enterolobii</i> isolates on Upland cotton germplasm lines having resistance quantitative trait loci (QTL) to RKN (M240 RNR, MRk-Rn-1) and/or reniform nematode (<i>Rotylenchulus reniformis</i>) (M713 Ren1, MRk-Rn-1) in comparison to their susceptible recurrent parents (DPL61, SG747). Multiple assays using eggs or J2 as inoculum demonstrated that both isolates reproduced equally well on all germplasm lines, producing reproductive factor (RF) values ≥ 6 on the otherwise nematode-resistant lines. Measurements of seedling growth in control and inoculated containers suggested that existing nematode-resistance QTL may offer a level of tolerance to <i>M. enterolobii</i> infection that should be further explored in greenhouse and field environments. <i>Meloidogyne enterolobii</i> infection of SG747 and MRk-Rn-1 showed nearly identical stages of symptom and nematode development over a time-course of 24 days. These data demonstrate that existing RKN and RN resistance QTL available in elite cotton varieties to producers are most likely insufficient in preventing yield loss due to <i>M. enterolobii</i> and that future research should focus on (i) understanding the <i>M. enterolobii</i>-cotton interaction at the molecular level, and (ii) screening novel germplasm collections to identify resistance loci.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230021"},"PeriodicalIF":1.3,"publicationDate":"2023-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10288305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10602706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First Reports and Morphological and Molecular Characterization of Pratylenchus delattrei and Quinisulcius capitatus Associated with Chickpea in Ethiopia. 埃塞俄比亚与鹰嘴豆相关的Pratylenchus delattrei和Quinisulcius capitatus的首次报道及其形态学和分子特征。
IF 1.3 4区 生物学
Journal of nematology Pub Date : 2023-06-11 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0027
Habtamu Kefelegn, Beira Hailu Meressa, Sunheng Yon, Marjolein Couvreur, Wim M L Wesemael, Misghina G Teklu, Wim Bert
{"title":"First Reports and Morphological and Molecular Characterization of <i>Pratylenchus delattrei</i> and <i>Quinisulcius capitatus</i> Associated with Chickpea in Ethiopia.","authors":"Habtamu Kefelegn, Beira Hailu Meressa, Sunheng Yon, Marjolein Couvreur, Wim M L Wesemael, Misghina G Teklu, Wim Bert","doi":"10.2478/jofnem-2023-0027","DOIUrl":"10.2478/jofnem-2023-0027","url":null,"abstract":"<p><p>Chickpea (<i>Cicer arietinum</i> L.) is classed among the most important leguminous crops of high economic value in Ethiopia. Two plant-parasitic nematode species, <i>Pratylenchus delattrei</i> and <i>Quinisulcius capitatus</i>, were recovered from chickpea-growing areas in Ethiopia and characterized using molecular and morphological data, including the first scanning electron microscopy data for <i>P. delattrei</i>. New sequences of D2-D3 of 28S, ITS rDNA and mtDNA <i>COI</i> genes have been obtained from these species, providing the first <i>COI</i> sequences for <i>P. delattrei</i> and <i>Q. capitatus,</i> with both species being found for the first time on chickpea in Ethiopia. Furthermore, <i>Pratylenchus delattrei</i> was recovered in Ethiopia for the first time. The information obtained about these nematodes will be crucial to developing effective nematode management plans for future chickpea production.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230027"},"PeriodicalIF":1.3,"publicationDate":"2023-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10258564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10290949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rotation of Cotton (Gossypium hirsutum) Cultivars and Fallow on Yield and Rotylenchulus reniformis. 棉花(Gossypium hirsutum)品种的轮作和对产量和肾形Rotylenchulus reniformis的影响。
IF 1.4 4区 生物学
Journal of nematology Pub Date : 2023-06-06 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0024
Casiani Soto-Ramos, Terry A Wheeler, Jonathan Shockey, Cecilia Monclova-Santana
{"title":"Rotation of Cotton (<i>Gossypium hirsutum</i>) Cultivars and Fallow on Yield and <i>Rotylenchulus reniformis</i>.","authors":"Casiani Soto-Ramos, Terry A Wheeler, Jonathan Shockey, Cecilia Monclova-Santana","doi":"10.2478/jofnem-2023-0024","DOIUrl":"10.2478/jofnem-2023-0024","url":null,"abstract":"<p><p>A three-year rotation of cotton (<i>Gossypium hirsutum</i>) cultivars either resistant (R) or susceptible (S) to <i>Rotylenchulus reniformis</i> and fallow (F) was examined for effect on cotton yield and nematode density. In year 1, 2, and 3, the resistant cultivar (DP 2143NR B3XF) yielded 78, 77, and 113% higher than the susceptible cultivar (DP 2044 B3XF). Fallow in year 1 followed by S in year 2 (F1S2) improved yield in year 2 by 24% compared with S1S2, but not as much as R1S2 (41% yield increase over S1S2). One year of fallow followed by R (F1R2) had lower yield in year 2 (11% reduction) than R1R2. The highest yield after three years of these rotations occurred with R1R2R3, followed by R1S2R3 (17% less yield) and F1F2S3 (35% less yield). <i>Rotylenchulus reniformis</i> density in soil averaged 57, 65, and 70% lower (year 1, 2, 3, respectively) in R1R2R3 compared with S1S2S3. In years 1 and 2, LOG<sub>10</sub> transformed nematode density (LREN) was lower in F1, and F1F2, than for all other combinations. In year 3, the lowest LREN were associated with R1R2R3, F1S2F3, and F1F2S3. The highest LREN were associated with F1R2S3, F1S2S3, S1S2S3, R1R2S3, and R1S2S3. The combination of higher yield and lower nematode density will be a strong incentive for producers to use the <i>R. reniformis</i> resistant cultivars continuously.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230024"},"PeriodicalIF":1.4,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10243494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10227766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reaction of Commercial Cultivars of Kiwifruit to Infection by Root-knot Nematode and Its Biocontrol Using Endophytic Bacteria. 猕猴桃商品品种对根结线虫感染的反应及其内生细菌的生物防治。
IF 1.3 4区 生物学
Journal of nematology Pub Date : 2023-06-05 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0020
Seyedeh Najmeh Banihashemian, Salar Jamali, Morteza Golmohammadi, Sina Noorizadeh, Mohammad Reza Atighi
{"title":"Reaction of Commercial Cultivars of Kiwifruit to Infection by Root-knot Nematode and Its Biocontrol Using Endophytic Bacteria.","authors":"Seyedeh Najmeh Banihashemian, Salar Jamali, Morteza Golmohammadi, Sina Noorizadeh, Mohammad Reza Atighi","doi":"10.2478/jofnem-2023-0020","DOIUrl":"10.2478/jofnem-2023-0020","url":null,"abstract":"<p><p>Root-knot nematodes (RKN) cause considerable economic losses to kiwifruit production annually. Screening of resistant cultivars has been one of the long-standing methods to manage root-knot nematodes. Here, the reaction of the four most common commercial cultivars of kiwifruit, namely, <i>Actinidia chinensis</i> var. <i>deliciosa</i> cv. Hayward, <i>A. chinensis</i> var. <i>deliciosa</i> cv. Abbott, <i>A. chinensis</i> var. <i>deliciosa</i> cv. Bruno, and <i>A. chinensis</i> var. <i>chinensis</i> cv. Haegeum (commonly known as 'Golden' kiwifruit) to infection by the RKN, <i>Meloidogyne incognita</i>, was evaluated. Among examined cultivars 'Golden' was the most susceptible, having on average 52.8 galls, 56.1 egg masses per gram of root, and 642 J2 population per 200 gram of soil. 'Bruno' showed the highest resistance, with 3.3 galls, 4.1 egg masses per gram of root, and 79 J2 in 200 g of soil. Then, two potential biological control agents, namely <i>Priestia megaterium</i> 31.en and <i>Agrobacterium tumefaciens</i> 19.en were used on 'Hayward' seedlings against <i>M. incognita</i> and showed a significant reduction in the number of galls and egg masses on roots, juvenile population in the soil, and increased the growth parameters of the plants compared to non-treated seedlings. We demonstrated that integrated management using resistant cultivars and biological control can provide a safe and economic method to control RKN, and these resistant cultivars can be used in breeding programs.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230020"},"PeriodicalIF":1.3,"publicationDate":"2023-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10241307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10239817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First Report of Direct Damage Caused by the Stubby-Root Nematode, Nanidorus minor, to Strawberry (Fragaria x ananassa), in Florida. 佛罗里达州草莓(Fragaria x ananassa)的小型短梗根线虫直接危害的首次报告。
IF 1.3 4区 生物学
Journal of nematology Pub Date : 2023-06-05 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0016
Clemen J Oliveira, Renato N Inserra, Johan A Desaeger
{"title":"First Report of Direct Damage Caused by the Stubby-Root Nematode, <i>Nanidorus minor</i>, to Strawberry (<i>Fragaria</i> x <i>ananassa</i>), in Florida.","authors":"Clemen J Oliveira, Renato N Inserra, Johan A Desaeger","doi":"10.2478/jofnem-2023-0016","DOIUrl":"10.2478/jofnem-2023-0016","url":null,"abstract":"<p><p>In 2019-2022, declining symptoms were observed in two commercial strawberry farms in Hillsborough County, Florida. The fields in the two farms consisted of raised beds covered by plastic mulch. Both were fumigated with a mixture of 1,3-dichloropropene (40%) + chloropicrin (60%) before planting. Samples collected from large patches with declining plants were infested with stubby-root nematodes. No sting and root-knot nematode species were detected. The results of morphological and molecular analyses indicated that the stubby-root nematode populations were representative of the species <i>Nanidorus minor</i>. The two cultivars 'Florida Brilliance' and 'Florida Sensation' in the two fields included plants with stubby root symptoms showing a reduction in the size of the root system and arrested growth and elongation of the feeder roots on the first strawberry crop. The nematode population densities in the two fields increased at the end of strawberry season and averaged 66 and 96 specimens in 200 cm<sup>3</sup> soil. In one of the fields, a second strawberry crop was established as in the previous year using the same practices (fumigation and raised beds covered with plastic). However, in this field the population of <i>N. minor</i> declined and did not reach damaging levels at the end of the season on the second strawberry crop. The factors causing the decline of the nematode population were not elucidated. This is the first report of a direct damaging effect of <i>N. minor</i> to strawberry.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230016"},"PeriodicalIF":1.3,"publicationDate":"2023-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10241308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10238283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of non-fumigant nematicides on reproduction and pathogenicity of Meloidogyne enterolobii and disease severity in tobacco. 非熏蒸杀线虫剂对烟根结线虫繁殖和致病性及烟草病害严重程度的影响。
IF 1.4 4区 生物学
Journal of nematology Pub Date : 2023-06-05 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0025
Md Shah Alam, Churamani Khanal, Joseph Roberts, William Rutter
{"title":"Impact of non-fumigant nematicides on reproduction and pathogenicity of <i>Meloidogyne enterolobii</i> and disease severity in tobacco.","authors":"Md Shah Alam, Churamani Khanal, Joseph Roberts, William Rutter","doi":"10.2478/jofnem-2023-0025","DOIUrl":"10.2478/jofnem-2023-0025","url":null,"abstract":"<p><p><i>Meloidogyne enterolobii</i> is a highly aggressive quarantine pathogen which threatens the multibillion-dollar tobacco industry and is not manageable with the currently available management methods in tobacco. There is currently no known host plant resistance in tobacco and previous studies have shown that the lower level of the currently recommended rate of non-fumigant nematicides does not provide satisfactory management of <i>M. enterolobii</i>. The current study was conducted with the hypothesis that <i>M. enterolobii</i> can be better managed using a single soil application of the maximum allowed rate of non-fumigant nematicides. Treatments involved three non-fumigant chemical nematicides (oxamyl, fluopyram, and fluensulfone), a biological nematicide derived from Burkholderia, and a non-treated control. Fluensulfone significantly suppressed the nematode reproduction relative to the control, the suppression being 71% for eggs and 86% for the second stage juveniles (J2). Fluopyram also suppressed nematode reproduction, although this was statistically insignificant, with the suppression being 26% and 37% for eggs and J2, respectively. Oxamyl significantly suppressed J2 (80%), but not eggs (50%) in relation to the control. The most significant reduction of disease severity was achieved by the application of fluensulfone (64%), followed by oxamyl (54%) and fluopyram (48%). Except for fluensulfone, which significantly reduced the root biomass, none of the nematicides significantly impacted root and shoot biomass. The biological nematicide did not significantly affect nematode reproduction, pathogenicity, or disease severity. The results from the current study suggest that while the non-fumigant nematicides provided a good level of the nematode suppression, more research is needed to improve the efficacy of non-fumigant nematicides through employing better application methods or finding better chemistries.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230025"},"PeriodicalIF":1.4,"publicationDate":"2023-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10241305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10238286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphological and Molecular Characterization of Talanema eshtiaghii sp. n. (Dorylaimida, Qudsianematidae) from Iran. 伊朗Talanema eshtiaghii sp.n.(Dorylaimida,Qudsianemalidae)的形态学和分子特征。
IF 1.3 4区 生物学
Journal of nematology Pub Date : 2023-06-05 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0022
Nasir Vazifeh, Gholamreza Niknam, Habibeh Jabbari, Reyes Peña-Santiago
{"title":"Morphological and Molecular Characterization of <i>Talanema eshtiaghii</i> sp. n. (Dorylaimida, Qudsianematidae) from Iran.","authors":"Nasir Vazifeh, Gholamreza Niknam, Habibeh Jabbari, Reyes Peña-Santiago","doi":"10.2478/jofnem-2023-0022","DOIUrl":"10.2478/jofnem-2023-0022","url":null,"abstract":"<p><p>A new species of the genus <i>Talanema</i>, recovered from the northwest of Iran, was described based on morphological, morphometric, and molecular data. <i>Talanema</i> eshtiaghii sp. n. was characterized by its 1.45-1.68 mm long body, lip region offset by constriction and 13-15 μm wide, odontostyle 15-18 μm long, double guiding ring, neck 312-362 μm long, pharyngeal expansion occupying 41-43% of the total neck length, uterus tripartite, and 111-189 μm long or 2.1-3.2 body diameters, vulva transverse (V = 55-58), tail similar in both sexes, conical with a dorsal concavity (30-44 μm, c = 33-56, c' = 1.0-1.6), spicules 49-56 μm long, and 14-18 shortly spaced ventromedian supplements in front of the level of the anterior end of spicules, with distinct hiatus. It was compared to four closely similar species, with emphasis on the most relevant traits to distinguish them. Molecular phylogenetic studies using partial sequence of the 28S rDNA (D2-D3 segment) revealed that the new species forms a clade with other currently sequenced representatives of <i>Talanema</i>, tentatively supporting the monophyly of this genus.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230022"},"PeriodicalIF":1.3,"publicationDate":"2023-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10241306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10238285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A New Biological Product Shows Promising Control of the Northern Root-Knot Nematode, Meloidogyne hapla, in Greenhouse Tomatoes. 一种新的生物制品有望控制温室番茄中的北方根结线虫。
IF 1.3 4区 生物学
Journal of nematology Pub Date : 2023-05-26 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0023
Elisabeth Darling, Abigail Palmisano, Henry Chung, Marisol Quintanilla-Tornel
{"title":"A New Biological Product Shows Promising Control of the Northern Root-Knot Nematode, <i>Meloidogyne hapla</i>, in Greenhouse Tomatoes.","authors":"Elisabeth Darling, Abigail Palmisano, Henry Chung, Marisol Quintanilla-Tornel","doi":"10.2478/jofnem-2023-0023","DOIUrl":"10.2478/jofnem-2023-0023","url":null,"abstract":"<p><p>Tomato plants are susceptible to significant yield losses when infested by the northern root-knot nematode, <i>Meloidogyne hapla.</i> While there are many options for conventional chemical management, few of these options offer effective control for organic growers or those who seek to adopt more environmentally considerate strategies. In this study, we showed that a new, biologically based product (referred to as \"MN21.2\") has potential for controlling populations of the northern root-knot nematode, <i>Meloidogyne hapla</i>, as a pest of susceptible tomato (cv. Rutgers) in a greenhouse trial. This is significant because if this product's efficacy is supported under field conditions, it may provide organic tomato growers with a valuable tool for fighting the plant-parasitic nematode pest, <i>M. hapla.</i></p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230023"},"PeriodicalIF":1.3,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10390845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10237492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus. 香蕉Hoplolaimus seinhorsti和Hoplolaimus pararobustus的特征(中国:Hoplolaimicae),以及Hoplolaimuns属的系统发育和物种划分。
IF 1.3 4区 生物学
Journal of nematology Pub Date : 2023-05-23 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0019
Emmanuel Olajide, Phougeishangbam Rolish Singh, Yao A Kolombia, Merlin Kornelia Rumbarar, Marjolein Couvreur, Wim Bert
{"title":"Characterization of <i>Hoplolaimus seinhorsti</i> and <i>Hoplolaimus pararobustus</i> (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus <i>Hoplolaimus</i>.","authors":"Emmanuel Olajide, Phougeishangbam Rolish Singh, Yao A Kolombia, Merlin Kornelia Rumbarar, Marjolein Couvreur, Wim Bert","doi":"10.2478/jofnem-2023-0019","DOIUrl":"10.2478/jofnem-2023-0019","url":null,"abstract":"<p><p>The morphological and molecular characterisations of two lance nematode species isolated from the rhizosphere of banana, <i>Hoplolaimus seinhorsti</i> and <i>H. pararobustus</i>, are provided based on an integrative study that includes light and scanning electron microscopy, phylogenetic analysis and two tree-based molecular species delimitation methods (GMYC and bPTP). Nineteen new sequences were obtained, including 5 partial 18S rRNA, 6 D2-D3 of 28S rRNA, 1 ITS rRNA and 7 <i>COI</i> mtDNA (the first <i>COI</i> sequences of <i>H. seinhorsti</i> and <i>H. pararobustus</i>), and an updated morphological character comparison of 37 <i>Hoplolaimus</i> species is presented. The tree-based molecular species-delimitation approaches employed gave markedly differing results, and also showed remarkable discrepancies among the investigated genes, although the bPTP output was found to agree well with established morphological species delimitations. Both species-delimitation approaches did, however, provide the same output for the <i>COI</i> mtDNA sequences, and the <i>COI</i> mtDNA gene sequence was also found to correspond better to established morphological species. It is therefore recommended by this paper as representing the most suitable barcode marker for <i>Hoplolaimus</i> species identification. This integrative study also resulted in the corrective reassignment of 17 gene sequences that were previously unidentified or incorrectly classified, as well as concluding that <i>H. pararobustus</i> consists of two cryptic species.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230019"},"PeriodicalIF":1.3,"publicationDate":"2023-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10586616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphological and Molecular Characterization of Diplogasteroides sp., a Cryptic Population of the Haslacheri Group (Diplogastridae), and Parasitorhabditis terebranus (Rhabditidae) from Korea. 韩国Haslacheri群(双壳虫科)的一个隐蔽种群——双壳拟杆菌属(Diplogastridae)和圆棒线虫(Rhabditidae)的形态学和分子特征。
IF 1.4 4区 生物学
Journal of nematology Pub Date : 2023-05-23 eCollection Date: 2023-02-01 DOI: 10.2478/jofnem-2023-0017
Abraham Okki Mwamula, Sang Myeong Lee, Young Hak Jung, Ho-Wook Lee, Yi Seul Kim, Young Ho Kim, Dong Woon Lee
{"title":"Morphological and Molecular Characterization of <i>Diplogasteroides</i> sp., a Cryptic Population of the <i>Haslacheri</i> Group (Diplogastridae), and <i>Parasitorhabditis terebranus</i> (Rhabditidae) from Korea.","authors":"Abraham Okki Mwamula, Sang Myeong Lee, Young Hak Jung, Ho-Wook Lee, Yi Seul Kim, Young Ho Kim, Dong Woon Lee","doi":"10.2478/jofnem-2023-0017","DOIUrl":"10.2478/jofnem-2023-0017","url":null,"abstract":"<p><p><i>Diplogasteroides</i> sp., a cryptic population of <i>D. haslacheri</i>, and <i>Parasitorhabditis terebranus</i> were reported from the frass of <i>Monochamus alternatus</i> galleries in dead <i>Pinus thunbergii</i> for the first time in Korea. Females and males are morphologically characterized and their linked DNA barcodes (18S-rRNA, 28S-rRNA, ITS-rRNA and COI) supplied. Females and males of the two species from Korea conform to the original species descriptions from Europe and the USA, with variations in a few details in morphometrics. Specifically, <i>Diplogasteroides</i> sp. is morphologically very similar to <i>D. haslacheri</i>. However, it cannot be designated as <i>D. haslacheri</i> due to the existence of cryptic species complex within the <i>haslacheri</i> group (<i>D. haslacheri</i>, <i>D. asiaticus, D. nix, D. andrassyi</i>, and <i>D. carinthiacus</i>), a condition requiring hybridization studies to test species identity within the group. Based on analysis of COI sequences, differences among these cryptic species are evident. Thus, in addition to hybridization tests, the COI might be a powerful DNA barcoding marker for the precise identification of these cryptic species within the genus. Additionally, this is the first molecular characterization of <i>P. terebranus</i>, and the species is herein recorded for the first time outside its type locality.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":"55 1","pages":"20230017"},"PeriodicalIF":1.4,"publicationDate":"2023-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277748/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10602703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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