Journal of nematology最新文献

{"title":"First reports of <i>Hemicycliophora poranga</i>, <i>Helicotylenchus dihystera</i> and <i>Tylenchorhynchus zeae</i> (Tylenchomorpha) from Greece and further records of four other nematode species.","authors":"Ilenia Clavero-Camacho, Alba N Ruiz-Cuenca, Carolina Cantalapiedra-Navarrete, Antonio Archidona-Yuste, Ioannis Giannakou, Maria Kormpi, Juan E Palomares-Rius, Pablo Castillo, Emmanuel A Tzortzakakis","doi":"10.2478/jofnem-2023-0044","DOIUrl":"10.2478/jofnem-2023-0044","url":null,"abstract":"<p><p>Nematode samplings in various areas and crops of Greece were carried out and the recovered nematode species were characterized using morphological and molecular data. Seven species of plant-parasitic nematodes were recovered, three of which are reported for the first time in Greece, including <i>Hemicycliophora poranga</i>, <i>Helicotylenchus dihystera</i> and <i>Tylenchorhynchus zeae</i>. Four other recovered species had already been reported in Greece, including <i>Bitylenchus hispaniensis</i>, <i>Helicotylenchus microlobus</i>, <i>Nanidorus minor</i> and <i>Scutellonema brachyurus</i>. D2-D3 segments of 28S rRNA gene for all of these nematode species are provided.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10657206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138460474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological, molecular, and histopathological studies on <i>Hadjelia truncata</i> from <i>Columba livia domestica</i> and its role as an environmental biological indicator.","authors":"Mohammed Albeshr, Rajwa Alsharief, Fatimah Al-Otibi, Esam M Al-Shaebi, Osama B Mohammed, Rewaida Abdel-Gaber","doi":"10.2478/jofnem-2023-0050","DOIUrl":"10.2478/jofnem-2023-0050","url":null,"abstract":"<p><p>Pigeons are a cosmopolitan group of birds with abundant and large populations associated with human activities. This study focused on determining parasitic infections within domestic pigeons (<i>Columba livia domestica</i>). Forty-eight pigeons were examined for infections, of which 29.16% were infected with a nematode parasite, identified as <i>Hadjelia truncata</i> (Habronematidae), under the koilin layer of their gizzards. The population of nematodes in infected gizzards did not exceed 20 adult worms. DNA from the gizzard worms was extracted and subjected to PCR using primers that amplify the partial 18S rDNA and cytochrome C oxidase subunit I (COX I) regions. Identification of this parasite based on microscopic study revealed the presence of trilobed lips with cephalic papillae and amphidial pores, as well as other characteristic features. In males, spicules were unequal with the presence of six pedunculated pairs of caudal papillae (4 pre- and 2 post-anal) and a tail surrounded with caudal ala. In females, the vulva was a rounded aperture located in front of the posterior end of the esophagus and uteri, which was filled with numerous embryonated eggs. DNA Sequences from partial 18S rDNA were homologous to sequences obtained from <i>H. truncata</i> in GenBank with a high percentage of identity. DNA sequences from mitochondrial gene COX I, however, were unique, and they were the first sequenced for <i>H. truncata</i>, since no sequences for this taxon were previously available in GenBank. Histopathological examination revealed enlargement of infected gizzards in comparison to non-infected ones, with the presence of necrosis and interstitial infiltration in the koilin layer. Concentrations of heavy metals (Fe, Cu, Zn, Cd, Cr, and Co) were measured using inductivity-coupled plasma in tissues (liver, muscles, and gizzards) from infected and non-infected pigeons as well as their parasites. Results showed different affinities of metals to tissues. Recovered parasites can minimize element concentration from their pigeon tissues. In Saudi Arabia, this study was considered the first report identifying pigeon nematodes and evaluating of the effects of their pathogenicity on the animals' welfare, as well as their application as a useful tool for monitoring environmental pollution.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138460476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nematode Genome Announcement: A Draft Genome of Seed Gall Nematode, <i>Anguina tritici</i>.","authors":"Ashish Kumar Singh, Antara Das, Ila Joshi, Manish Kumar, Deshika Kohli, Pankaj, Kishor Gaikwad, Pradeep Kumar Jain, Anil Sirohi","doi":"10.2478/jofnem-2023-0031","DOIUrl":"10.2478/jofnem-2023-0031","url":null,"abstract":"<p><p><i>Anguina tritici</i> is the first plant-parasitic nematode described in literature, dating back to the year 1743. It is responsible for causing earcockle (seed gall) and tundu diseases in wheat and rye. Notably, this nematode has been observed to survive in an anhydrobiotic state for up to 32 years within wheat seed galls. These exceptional characteristics have inspired the sequencing of the <i>A. tritici</i> genome. In this study, we present the initial draft genome of <i>A. tritici</i>, obtained using the Illumina MiSeq platform with coverage of 60-fold. The genome is estimated to have a size of 164 Mb and comprises 39,965 protein-coding genes, exhibiting a GC content of 39.1%. The availability of this genome data will serve as a foundation for future functional biological investigations, particularly for genes whose functions remain unknown to this day.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138460477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Root-Knot Nematode species, <i>Meloidogyne karsseni</i> n. sp. (Nematoda: Meloidogynidae), From Mexico and a Taxonomic Update on <i>M. paranaensis</i> From Guatemala.","authors":"Phougeishangbam Rolish Singh, Denis Gitonga, Abolfazl Hajihassani, Adriaan Verhage, Eveline van Aalst-Philipse, Marjolein Couvreur, Wim Bert","doi":"10.2478/jofnem-2023-0042","DOIUrl":"10.2478/jofnem-2023-0042","url":null,"abstract":"<p><p>A new root-knot nematode (RKN) species, <i>Meloidogyne karsseni</i> n. sp., associated with sweet pepper from Mexico, and a population of <i>M. paranaensis</i> from Guatemala, are described using data from morphological, biochemical (isozyme enzymes), molecular, and phylogenetic analyses. <i>Meloidogyne karsseni</i> n. sp. can be morphologically diagnosed using the combined features of the second-stage juveniles, viz. body length (345 to 422 μm), a conical rounded head region, a post-labial annule lacking transverse striation, a thin stylet 11 to 12 μm long, rounded to oval and backwardly sloping knobs, dorsal gland orifice (DGO) at 5.2 to 6.0 μm from the knobs, a hemizonid just above the secretory-excretory (SE) pore, a tapering tail with finely rounded terminus and one or two very weak constrictions at hyaline tail tip; the female characters viz. oval-to-rounded perineal pattern with coarse striation on lateral sides around the anus, low dorsal arch with finer striations, and distinctly visible lateral lines; and the male characteristics viz. a rounded and continuous head, a post-labial annule without transverse striations, a robust stylet 20 to 24 μm long, rounded-to-oval and slightly backwardly sloping knobs, and a DGO at 2.4 to 2.9 μm from the knobs. In all the studied males of <i>M. paranaensis</i>, a characteristic sclerotization around the duct of SE-pore was also observed for the first time. Sequences of 18S, D2-D3 of 28S, and ITS of rDNA, and <i>cox</i>1 of mtDNA were generated for the two species, and in the phylogenetic trees based on these genes, both species appeared in the tropical RKN species complex clade.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10590206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49690973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Management of Reniform Nematode in Cotton Using Winter Crop Residue Amendments Under Greenhouse Conditions.","authors":"Rebeca Sandoval-Ruiz, Zane J Grabau","doi":"10.2478/jofnem-2023-0041","DOIUrl":"10.2478/jofnem-2023-0041","url":null,"abstract":"<p><p><i>Rotylenchulus reniformis</i> (reniform nematode, RN) is among the most important nematodes affecting cotton. Cultural practices, such as rotation and soil amendment, are established methods for managing RN. Management may be enhanced if crop residue has biofumigant properties against RN. The objective was to evaluate the efficacy of winter crop amendments for managing RN in the greenhouse. Reniform nematode-infested soil was amended with dry or fresh organic matter (OM, 2% w/w) from winter crops - canola, carinata, hairy vetch, oat, or no crop. Cotton was subsequently grown in this soil. Independent of the crop, dry OM amendments were more effective than no amendment at managing RN, while fresh OM amendments were not. Soil and root RN abundances and reproduction factors were generally lower in Trials 1 and 3 for dry OM than fresh OM amendments or control without OM. In Trial 2, none of the OM treatments reduced RN parameters compared with no OM control. In general, when compared to plants without RN or OM, RN did not produce significant changes in growth parameters but did affect physiology (Soil Plant Analysis Development, or SPAD, values). In conclusion, dry OM amendments can help manage RN, crop growth does not always relate to RN abundances, and SPAD values could help indicate RN presence.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10590205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49690974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tomato <i>Mi</i>-gene Resistance-Breaking Populations of <i>Meloidogyne</i> Show Variable Reproduction on Susceptible and Resistant Crop Cultivars.","authors":"A T Ploeg, C S Stoddard, T A Turini, J J Nunez, E M Miyao, S A Subbotin","doi":"10.2478/jofnem-2023-0043","DOIUrl":"10.2478/jofnem-2023-0043","url":null,"abstract":"<p><p>Sixteen <i>Meloidogyne</i> isolates from tomato fields in California grown with resistant cultivars were multiplied on resistant tomato in a greenhouse. Of these resistance-breaking isolates, one was identified as <i>M. javanica</i>, and all others as <i>M. incognita.</i> The reproduction of the <i>M. javanica</i> isolate and four <i>M. incognita</i> isolates on six resistant tomato cultivars and on susceptible and resistant cultivars of pepper, sweetpotato, green bean, cotton, and cowpea was evaluated and compared to an avirulent <i>M. incognita</i> population in greenhouse pot trials. On resistant tomato cultivars, there were minor but significant differences between the resistance-breaking <i>Meloidogyne</i> isolates and between the different tomato cultivars. Of the other resistant crop cultivars, pepper was resistant to all isolates and green bean to all <i>M. incognita</i> isolates, while cotton and cowpea allowed reproduction of one of the resistance-breaking <i>M. incognita</i> isolates. The resistant sweetpotato cv. Bonita behaved like resistant tomato, allowing reproduction of all five resistance-breaking isolates but not of the avirulent <i>M. incognita</i>. Our results showed that variability exists among resistance-breaking <i>Meloidogyne</i> isolates, and that isolates overcoming resistance in tomato may also be virulent on resistant sweetpotato.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10578805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41236095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Society of Nematologists 1961.","authors":"","doi":"10.2478/jofnem-2023-0047","DOIUrl":"10.2478/jofnem-2023-0047","url":null,"abstract":"","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10578804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41236094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant Parasitic Nematode Identification in Complex Samples with Deep Learning.","authors":"Sahil Agarwal, Zachary C Curran, Guohao Yu, Shova Mishra, Anil Baniya, Mesfin Bogale, Kody Hughes, Oscar Salichs, Alina Zare, Zhe Jiang, Peter DiGennaro","doi":"10.2478/jofnem-2023-0045","DOIUrl":"10.2478/jofnem-2023-0045","url":null,"abstract":"<p><p>Plant parasitic nematodes are significant contributors to yield loss worldwide, causing devastating losses to every crop species, in every climate. Mitigating these losses requires swift and informed management strategies, centered on identification and quantification of field populations. Current plant parasitic nematode identification methods rely heavily on manual analyses of microscope images by a highly trained nematologist. This mode is not only expensive and time consuming, but often excludes the possibility of widely sharing and disseminating results to inform regional trends and potential emergent issues. This work presents a new public dataset containing annotated images of plant parasitic nematodes from heterologous soil extractions. This dataset serves to propagate new automated methodologies or speedier plant parasitic nematode identification using multiple deep learning object detection models and offers a path towards widely shared tools, results, and meta-analyses.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10578830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41236093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Virulence Phenotypes of <i>Heterodera glycines</i> during 2020 in Indiana.","authors":"Ricky Critchfield, Jaden King, John Bonkowski, Darcy Telenko, Tom Creswell, Lei Zhang","doi":"10.2478/jofnem-2023-0039","DOIUrl":"10.2478/jofnem-2023-0039","url":null,"abstract":"<p><p>The soybean cyst nematode (SCN, <i>Heterodera glycines</i>) is the most yield-limiting pathogen of soybean in the US. This study was carried out in order to provide updated information on SCN virulence phenotypes in Indiana. A total of 124 soil samples were collected from soybean fields in 2020 and all of them tested positive for SCN. The virulence phenotypes of 42 representative SCN populations were determined with seven soybean indicator lines using the standard HG type test. The most predominant HG types were 2.5.7 and 1.2.5.7, which accounted for 64% and 14% of the SCN populations tested, respectively. None of the SCN populations tested were rated as HG type 0, compared with 28% of the populations in a previous survey in Indiana during 2006-2008. Nearly 88% of the SCN populations evaluated in this study overcame the resistance provided by PI 88788, which is the most common source of resistance in soybean, up from 56% in the 2006-2008 survey. Approximately 14% of SCN populations tested were virulent to PI 548402 (Peking), in contrast to 0% in the 2006-2008 survey. This study reveals a trend of increasing virulence of SCN populations to resistant sources of soybean in Indiana. The results highlighted the importance of rotating soybean varieties with different types of resistance and identifying new sources of resistance for sustainable management of SCN.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10577647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41236092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reniform Nematode Management Using Winter Crop Rotation and Residue Incorporation Methods in Greenhouse Experiments.","authors":"Rebeca Sandoval-Ruiz, Zane J Grabau","doi":"10.2478/jofnem-2023-0035","DOIUrl":"10.2478/jofnem-2023-0035","url":null,"abstract":"<p><p><i>Rotylenchulus reniformis</i> (reniform nematode, RN) is an important pathogen in cotton production. Cultural practices such as crop rotation and biofumigation-management of soil pathogens by biocidal compounds from crop residues-may help manage RN. The objective of this study was to evaluate the efficacy of winter crops for RN management through combinations of rotation and crop residue incorporation in a cotton greenhouse experiment. A total of 10 treatments were evaluated in soil inoculated with RN: three winter crops (carinata, oat, or hairy vetch) grown in rotation with no shoot organic matter (OM) incorporated (1-3), fresh shoot OM incorporated (4-6), or dry shoot OM incorporated (7-9), and a fallow control (10). Roots were re-incorporated in all treatments except fallow. Subsequently, cotton was grown. Oat and fallow were better rotation crops to lower soil RN abundances at winter crop termination than hairy vetch and carinata. After the OM incorporation treatments and cotton growth, oat was generally more effective at managing RN in cotton than carinata or hairy vetch. Within each crop, incorporation treatment generally did not affect RN management. Cotton growth was not consistently affected by the treatments.</p>","PeriodicalId":16475,"journal":{"name":"Journal of nematology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10499337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10272922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}