Xuemei Bai, Zhijie Wang, Hongbo Xiong, Chipeng Yan, Yufeng Yao, Chengqi Xu, Hui Li, Qing K Wang
{"title":"Molecular mechanism for the interaction of MOG1 with the intracellular loop II of cardiac sodium channel Na<sub>v</sub>1.5 and its role in arrhythmias.","authors":"Xuemei Bai, Zhijie Wang, Hongbo Xiong, Chipeng Yan, Yufeng Yao, Chengqi Xu, Hui Li, Qing K Wang","doi":"10.1016/j.yjmcc.2025.06.005","DOIUrl":"https://doi.org/10.1016/j.yjmcc.2025.06.005","url":null,"abstract":"<p><p>SCN5A encodes the cardiac sodium channel α-subunit Na<sub>v</sub>1.5, and its variants cause long QT syndrome (LQTS), Brugada syndrome (BrS) and other arrhythmias. MOG1 interacts with Na<sub>v</sub>1.5 to increase cardiac sodium current densities, however, molecular mechanisms remain poorly defined. The objectives of this study were to identify the crucial structural elements responsible for the interaction between MOG1 and Na<sub>v</sub>1.5 intracellular Loop II, and determine the significance of this interaction to cardiac arrhythmias. Whole-cell patch-clamping was used to record sodium current I<sub>Na</sub> in tsA201 and neonatal rat primary cardiomyocytes. Glutathione S-transferase (GST) pull-down assays were used to characterize protein-protein interactions. Mutagenesis was used to create deletions and point mutations. Characterization of large deletions and small deletions of Na<sub>v</sub>1.5 Loop II 940-1200 defined the MOG1-interacting domain to V1190-H1200. Point mutation analysis revealed that amino acids R<sub>1195</sub>, Y<sub>1199</sub> and H<sub>1200</sub> were involved in MOG1-Na<sub>v</sub>1.5 Loop II interaction. Two variants of MOG1-interacting domain from human patients showed important functional effects. Variant p.R1195C was identified in two individuals with cardiac arrhythmias in ClinVar, weakened the interaction between Na<sub>v</sub>1.5 and MOG1, and reduced MOG1-enhanced cardiac sodium current densities. Variant p.Y1199S was identified in one individual with LQTS and one with cardiac arrhythmias, generated late I<sub>Na</sub>, weakened the interaction between Na<sub>v</sub>1.5 and MOG1, and reduced MOG1-enhanced cardiac sodium current densities. This study identifies three critical amino acids R<sub>1195</sub>, Y<sub>1199</sub> and H<sub>1200</sub> of Na<sub>v</sub>1.5 Loop II for interaction with MOG1, and reveals the molecular mechanisms by which variants p.R1195C and p.Y1199S in MOG1-interacting domain cause LQTS and cardiac arrythmias.</p>","PeriodicalId":16402,"journal":{"name":"Journal of molecular and cellular cardiology","volume":" ","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thomas G. Martin , Stephen J. Langer , Claudia Crocini , Eunhee Chung , Leslie A. Leinwand
{"title":"Activation of FoxO1 prevents and reverses cardiac hypertrophy from diverse stimuli","authors":"Thomas G. Martin , Stephen J. Langer , Claudia Crocini , Eunhee Chung , Leslie A. Leinwand","doi":"10.1016/j.yjmcc.2025.06.008","DOIUrl":"10.1016/j.yjmcc.2025.06.008","url":null,"abstract":"<div><div>The heart is a dynamic organ capable of structural and functional remodeling in the wake of changing mechanical and/or circulating cues. While the molecular underpinnings of cardiac hypertrophy are well-defined, the mechanisms of hypertrophy regression following stimulus removal are relatively less understood. Here, we demonstrate that activation of forkhead box proteins (FoxOs), and increased expression of their autophagy gene targets, are common features of hypertrophy regression after both exercise and pregnancy in mice. Additionally, we show FoxO1 activation is sufficient to prevent and reverse adrenergic agonist-dependent pathological hypertrophy. Our findings highlight the central role of FoxO1 in regulating cardiac mass.</div></div>","PeriodicalId":16402,"journal":{"name":"Journal of molecular and cellular cardiology","volume":"205 ","pages":"Pages 62-67"},"PeriodicalIF":4.9,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Javad Habibi , Vincent G. DeMarco , Dongqing Chen , Adam Whaley-Connell , Michael A. Hill , Guanghong Jia
{"title":"Endothelial CD36 mediates diet-induced increases in aortic stiffness","authors":"Javad Habibi , Vincent G. DeMarco , Dongqing Chen , Adam Whaley-Connell , Michael A. Hill , Guanghong Jia","doi":"10.1016/j.yjmcc.2025.06.009","DOIUrl":"10.1016/j.yjmcc.2025.06.009","url":null,"abstract":"<div><div>A Western diet (WD) contributes to the rising prevalence of obesity and insulin resistance, both of which are key risk factors for arterial stiffening and related cardiovascular diseases. We recently found that elevated CD36 is associated with increased ectopic lipid accumulation, systemic and tissue insulin resistance, and arterial stiffening. Here, we further examined whether endothelial cell (EC) specific CD36 (ECCD36) participates in WD-induced aortic insulin resistance, lipid accumulation, inflammation, fibrosis, remodeling, and associated aortic stiffening. Female ECCD36 knockout (ECCD36<sup>−/−</sup>) and wild-type (ECCD36<sup>+/+</sup>) mice, at six weeks of age, were fed either a Western diet (WD) or a standard chow diet (CD) for 16 weeks. Aortic stiffness and activity were investigated by ultrasound (pulse wave velocity) and wire myography, respectively. Gene expression was monitored by western blot and quantitative PCR. Lipid content and aortic remodeling were explored by Oil red O staining and immunostaining, respectively. 16 weeks of WD increased aortic stiffening that was associated with vascular insulin resistance and reduced insulin metabolic signaling via phosphoinositide 3-kinases/protein kinase B. The pathophysiological changes in vascular insulin resistance and stiffening were associated with activation of mammalian target of rapamycin/S6 kinase signaling, increased lipid disorders, decreased tight junction-associated protein occludin, and increased proinflammatory response, and aortic remodeling. These abnormalities were blunted in ECCD36<sup>−/−</sup> mice fed a WD. These findings suggest that under an obesogenic Western diet (WD), heightened ECCD36 signaling contributes to aortic insulin resistance, increased lipid accumulation, increased endothelial permeability and proinflammatory responses, fibrosis, vascular remodeling, and consequent aortic stiffening.</div></div>","PeriodicalId":16402,"journal":{"name":"Journal of molecular and cellular cardiology","volume":"205 ","pages":"Pages 52-61"},"PeriodicalIF":4.9,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jose Alberto Navarro-Garcia , Joshua A. Keefe , Jia Song , Na Li , Xander H.T. Wehrens
{"title":"Mechanisms underlying atrial fibrillation in chronic kidney disease","authors":"Jose Alberto Navarro-Garcia , Joshua A. Keefe , Jia Song , Na Li , Xander H.T. Wehrens","doi":"10.1016/j.yjmcc.2025.06.002","DOIUrl":"10.1016/j.yjmcc.2025.06.002","url":null,"abstract":"<div><div>Chronic kidney disease (CKD) is a serious and progressive worldwide health problem affecting 15 % of the global population. CKD is associated with higher mortality rates due to secondary complications such as cardiovascular disease. Common cardiovascular complications found in CKD patients include left ventricular hypertrophy, heart failure, and cardiac arrhythmias. The most common type of cardiac arrhythmia in CKD patients is atrial fibrillation (AF). Proper management of AF is important due to its high risk of cardiovascular complications and stroke. The incidence of AF remains higher in CKD patients than in the healthy population, highlighting the need to improve our understanding of the mechanisms underlying CKD-induced AF. In this review, we discuss well-known systemic factors linking CKD to AF pathogenesis. We highlighted the involvement of several inflammatory mediators in the CKD-induced atrial arrhythmogenesis. We also address special considerations for experimental models of CKD and AF management in CKD patients. Finally, we emphasize the need for a deeper understanding of the molecular underpinning, and for high-quality clinical investigations into the CKD-AF connection.</div></div>","PeriodicalId":16402,"journal":{"name":"Journal of molecular and cellular cardiology","volume":"205 ","pages":"Pages 37-51"},"PeriodicalIF":4.9,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seulhee Kim , Hwayeon Lim , Patrick Ernst , Li Zhu , Jiashuai Zhang , Min Xie , Xiaoguang “Margaret” Liu , Lufang Zhou
{"title":"Optogenetic mitochondrial preconditioning enhances cardiomyocyte survival under stress","authors":"Seulhee Kim , Hwayeon Lim , Patrick Ernst , Li Zhu , Jiashuai Zhang , Min Xie , Xiaoguang “Margaret” Liu , Lufang Zhou","doi":"10.1016/j.yjmcc.2025.06.004","DOIUrl":"10.1016/j.yjmcc.2025.06.004","url":null,"abstract":"<div><div>Mitochondria play a central role in preconditioning-mediated cytoprotection, yet the specific role of mitochondrial membrane potential (ΔΨ<sub>m</sub>) in this process remains incompletely understood. In this study, we employed a next-generation, mitochondrial-targeted optogenetic system (mOpto) to induce precisely controlled (partial and transient) ΔΨ<sub>m</sub> depolarization and investigate its role in enhancing cardiomyocyte resilience to stress. Human AC16 cardiomyocytes expressing mOpto were subjected to low-intensity LED illumination for preconditioning, followed by exposure to stressors including FCCP, H<sub>2</sub>O<sub>2</sub>, or simulated ischemia-reperfusion. mOpto-preconditioned cells exhibited significantly improved viability, attenuated ΔΨ<sub>m</sub> depolarization, and reduced reactive oxygen species (ROS) production compared to non-preconditioned controls. Notably, this cytoprotective effect occurred independently of canonical ROS signaling and mitochondrial ATP-sensitive potassium channel (mitoK<sub>ATP</sub>) activation. Transcriptional analysis revealed coordinated mitochondrial and metabolic reprogramming, including upregulation of genes involved in lipid biosynthesis, mitochondrial quality control, energy homeostasis, and a shift toward mitochondrial fusion. Importantly, mOpto preconditioning conferred similar cytoprotective effects in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), underscoring the translational potential of this approach. These findings demonstrate that mOpto-mediated transient ΔΨ<sub>m</sub> depolarization induces a preconditioning effect that enhances cardiomyocyte resilience through the establishment of a mitochondrial “memory” and dynamic remodeling of mitochondrial function.</div></div>","PeriodicalId":16402,"journal":{"name":"Journal of molecular and cellular cardiology","volume":"205 ","pages":"Pages 24-36"},"PeriodicalIF":4.9,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144263399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Margaretha A.J. Morsink , Josephine M. Watkins , Katelyn Zhu , Xiaokan Zhang , Lori J. Luo , Barry M. Fine , Bryan Z. Wang , Gordana Vunjak-Novakovic
{"title":"The BAG3-HSP70-CHIP axis controls the degradation of TGFBR2 in cardiac fibroblasts","authors":"Margaretha A.J. Morsink , Josephine M. Watkins , Katelyn Zhu , Xiaokan Zhang , Lori J. Luo , Barry M. Fine , Bryan Z. Wang , Gordana Vunjak-Novakovic","doi":"10.1016/j.yjmcc.2025.06.003","DOIUrl":"10.1016/j.yjmcc.2025.06.003","url":null,"abstract":"<div><div>Transforming Growth Factor Beta (TGF-β) is a master regulator of cardiac fibrosis, in part through the type II TGF-β receptor (TGFBR2) which initiates signaling after ligand binding. We previously identified the co-chaperone protein Bcl2-associated athanogene (BAG3) as a modulator of TGFBR2 through ubiquitination and proteasomal degradation. However, the E3 ligase of TGFBR2 was not known. Using induced pluripotent stem cell-derived cardiac fibroblasts, we identified C-terminal interacting protein of HSP70 (CHIP) as an E3 ubiquitin ligase utilized by BAG3 for TGFBR2 degradation in cardiac fibroblasts. Overexpression of CHIP significantly decreased TGFBR2 stability, while inhibition of CHIP led to increased sensitivity to TGF-β and subsequent promotion of a fibrogenic program. Further, the BAG3-HSP70 interaction was crucial to this process, as disruption of the axis increased TGFBR2 stability and sensitivity to TGF-β signaling. Together, these findings demonstrate that the BAG3-HSP70-CHIP axis controls TGF-β signaling in cardiac fibroblasts and could serve as a new therapeutic target for cardiac fibrosis.</div></div>","PeriodicalId":16402,"journal":{"name":"Journal of molecular and cellular cardiology","volume":"205 ","pages":"Pages 13-23"},"PeriodicalIF":4.9,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cytoplasmic mutant RBM20 causes arrhythmogenicity in murine atria.","authors":"Kensuke Ihara , Satoshi Iwamiya , Masaki Ikuta , Yurie Soejima , Yuichi Hiraoka , Atsushi Nakano , Susumu Minamisawa , Tetsushi Furukawa , Hidehito Kuroyanagi , Tetsuo Sasano","doi":"10.1016/j.yjmcc.2025.06.001","DOIUrl":"10.1016/j.yjmcc.2025.06.001","url":null,"abstract":"<div><div>RNA binding motif protein 20 (RBM20) is a critical splicing regulator in cardiomyocytes, and mutations in its RSRSP domain are associated with severe dilated cardiomyopathy (DCM) and a high prevalence of atrial fibrillation (AF). RBM20 mutation has long been thought to cause DCM through the disturbed splicing of the target genes by its loss of function. However, recent studies have highlighted that the gain of function of mutant RBM20, independent of splicing defects, may also play a critical role in the pathogenesis of DCM. Despite these findings, the contribution of the gain of function of mutant RBM20 to the development of AF remains poorly understood. In this study, we aimed to elucidate the contribution of mutant RBM20 in atrial arrhythmogenicity by generating a novel atrial-specific mutant RBM20-expressing mouse model (<em>Sln</em><sup><em>Cre/+</em></sup>; LSL-<em>Rbm20</em><sup><em>S637A</em></sup> mice). These mice specifically expressed mutant RBM20 in the atria while maintaining RBM20-dependent alternative splicing. Analyses revealed the spontaneous development of atrial tachycardia and increased inducibility of AF, despite the absence of atrial structural remodeling or heart failure in <em>Sln</em><sup><em>Cre/+</em></sup>; LSL-<em>Rbm20</em><sup><em>S637A</em></sup> mice. Reduced atrial conduction velocity was observed, along with decreased and mislocalized expression of connexin 43, as well as abnormal Ca<sup>2+</sup> handling and altered phosphorylation of Ca<sup>2+</sup>-handling proteins. These findings suggest that mutant RBM20 contributes to the arrhythmogenicity through mechanisms independent of splicing regulation, involving alterations in Ca<sup>2+</sup> handling and electrical conduction property in murine atria.</div></div>","PeriodicalId":16402,"journal":{"name":"Journal of molecular and cellular cardiology","volume":"205 ","pages":"Pages 1-12"},"PeriodicalIF":4.9,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xijian Ke , Jonathan S. Baillie , Enrico D. Lemma , Martin Bastmeyer , Markus Hecker , Nina D. Ullrich
{"title":"The role of connexin-43 in modeling arrhythmogenic diseases with induced pluripotent stem cell-derived cardiomyocytes","authors":"Xijian Ke , Jonathan S. Baillie , Enrico D. Lemma , Martin Bastmeyer , Markus Hecker , Nina D. Ullrich","doi":"10.1016/j.yjmcc.2025.05.008","DOIUrl":"10.1016/j.yjmcc.2025.05.008","url":null,"abstract":"<div><div>A common pathophysiological characteristic of arrhythmic diseases is the disruption of electrical signal transmission across the heart causing life-threatening rhythm disorders. These conditions are associated with decreased expression of connexin-43 (Cx43) at intercalated discs and its translocation to the lateral membranes, however, the underlying mechanisms remain unclear. Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM) offer a model for studying these pathophysiological processes. Here, we tested the hypothesis that chronic stress, usually preceding arrhythmic developments, modulates Cx43 expression. iPSC-CM were electrically stimulated at a normal rate and by tachypacing, and their electrical and Ca<sup>2+</sup> signaling properties were analyzed. Our data revealed that tachypacing significantly reduced Cx43 expression by a micro-RNA miR-1-dependent mechanism. Anti-miR-1 treatment restored Cx43 expression in conditions of stress, enhanced Na<sup>+</sup> currents, improved Ca<sup>2+</sup> propagation and synchronized electrical activity. These findings suggest miR-1 as a potential pharmacological target for mitigating arrhythmogenic remodeling and restoring robust electrical signal transmission in cardiomyocytes.</div></div>","PeriodicalId":16402,"journal":{"name":"Journal of molecular and cellular cardiology","volume":"204 ","pages":"Pages 79-88"},"PeriodicalIF":4.9,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144154692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kyung Chan Park , Amir Mashia Jaafari , Christopher Anthony Smith , Althea Rennisa Lobo , Lorenzo Errichelli , Gül Şimşek , Mala Gunadasa-Rohling , Alexander Marchant , Maria O. Levitin , Virginia Castilla-Llorente , Patrick Vilela , Pawel Swietach
{"title":"A Langendorff-heart discovery pipeline demonstrates cardiomyocyte targeting by extracellular vesicles functionalized with beta-blockers using click-chemistry","authors":"Kyung Chan Park , Amir Mashia Jaafari , Christopher Anthony Smith , Althea Rennisa Lobo , Lorenzo Errichelli , Gül Şimşek , Mala Gunadasa-Rohling , Alexander Marchant , Maria O. Levitin , Virginia Castilla-Llorente , Patrick Vilela , Pawel Swietach","doi":"10.1016/j.yjmcc.2025.05.007","DOIUrl":"10.1016/j.yjmcc.2025.05.007","url":null,"abstract":"<div><div>Extracellular vesicles (EVs) are widely explored as vehicles for delivering therapeutic or experimental cargo to cardiomyocytes. Efforts to improve EV bioavailability in the heart, and reduce their off-target actions, require screening methods that can replicate the physiological and anatomical barriers present in the myocardium. Additionally, discovery pipelines must exercise control over EV dosage and timing, and provide a means of assessing cargo incorporation into cardiomyocytes specifically. These criteria are not generally met by experiments on cultured cells or animals. Here, we present a Langendorff-heart discovery pipeline that combines the strengths of <em>in vivo</em> and <em>in vitro</em> approaches. Langendorff-mode perfusion enables controlled exposure of beating hearts to re-circulated EVs. Following perfusion, cardiomyocytes can be isolated enzymatically for analysis, such as imaging. We tested this discovery pipeline by functionalizing EVs with beta-blockers (atenolol, metoprolol) using click-chemistry and incorporating the fluorescent protein NeonGreen2 to track the fate of EV cargo. Fluorescence in cardiomyocytes, including their nuclear regions, increased after Langendorff-treatment with beta-blocker decorated EVs, but only if these contained NeonGreen2, implicating the fluorescent cargo as the source of signal. Superior binding efficacy of beta-blockers was confirmed by referencing to the substantially lower signals obtained using wild-type EVs or EVs presenting myomaker or myomixer proteins, motifs that modestly enrich cardiac EV uptake in mice. Our findings demonstrate successful cardiomyocyte targeting using EVs decorated with beta-receptor binders. We propose the Langendorff-perfused heart as an intermediate step - nested between <em>in vitro</em> characterisation and animal testing - in discovery pipelines for seeking improved cardiac-specific EV designs.</div></div>","PeriodicalId":16402,"journal":{"name":"Journal of molecular and cellular cardiology","volume":"204 ","pages":"Pages 89-100"},"PeriodicalIF":4.9,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cristina M. Risi , Maicon Landim-Vieira , Betty Belknap , P. Bryant Chase , Jose R. Pinto , Vitold E. Galkin
{"title":"The role of the troponin T interactions with actin in regulation of cardiac thin filament revealed by the troponin T pathogenic variant Ile79Asn","authors":"Cristina M. Risi , Maicon Landim-Vieira , Betty Belknap , P. Bryant Chase , Jose R. Pinto , Vitold E. Galkin","doi":"10.1016/j.yjmcc.2025.05.005","DOIUrl":"10.1016/j.yjmcc.2025.05.005","url":null,"abstract":"<div><div>Cardiac muscle contraction/relaxation cycle depends on the rising and falling Ca<sup>2+</sup> levels in sarcomeres that control the extent of interactions between myosin-based thick and actin-based thin filaments. Cardiac thin filament (cTF) consists of actin, tropomyosin (Tm) that regulates myosin binding to actin, and troponin complex that governs Tm position upon Ca<sup>2+</sup>-binding. Troponin has three subunits – Ca<sup>2+</sup>-binding troponin C (TnC), Tm stabilizing troponin T (TnT), and inhibitory troponin I (TnI). TnT N-terminus (TnT1) interactions with actin stabilize the inhibited state of cTF. TnC, TnI, and Tm work in concert to control actomyosin interactions. Cryo-electron microscopy (cryo-EM) provided factual structures of healthy cTF, but structures of cTF carrying missense mutations linked to human cardiomyopathy are unknown. Variant Ile79Asn in human cardiac TnT (TnT-I79N) increases myofilament Ca<sup>2+</sup> sensitivity and slows cross-bridge kinetics, leading to severe hypertrophic/restrictive cardiomyopathy. Here, we used TnT-I79N mutation as a tool to examine the role of TnT1 in the complex mechanism of cTF regulation. Comparison of the cryo-EM structures of murine wild type and TnT-I79N native cTFs at systolic Ca<sup>2+</sup> levels (pCa = 5.8) demonstrates that TnT-I79N causes 1) dissociation of the TnT1 loop from its actin interface that results in Tm release to a more activated position, 2) reduced interaction of TnI C-terminus with actin-Tm, and 3) increased frequency of Ca<sup>2+</sup>-bound regulatory units. Our data indicate that the TnT1 loop is a crucial element of the allosteric regulatory network that couples Tn subunits and Tm to maintain adequate cTF response to physiological Ca<sup>2+</sup> levels during a heartbeat.</div></div>","PeriodicalId":16402,"journal":{"name":"Journal of molecular and cellular cardiology","volume":"204 ","pages":"Pages 55-67"},"PeriodicalIF":4.9,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144134291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}