Journal of Medical Virology最新文献

{"title":"Spatial Proteomics Using BiFCPL Identifies Regulators of DMV Formation Involved in Coronavirus Replication","authors":"Maoge Zhou,&nbsp;Jinping Yang,&nbsp;Buyun Tian,&nbsp;Xingyue Wang,&nbsp;Zihan Chen,&nbsp;Hang Yang,&nbsp;Bingjie Kong,&nbsp;Li Xiao,&nbsp;Zonghong Li","doi":"10.1002/jmv.70547","DOIUrl":"https://doi.org/10.1002/jmv.70547","url":null,"abstract":"<div>\u0000 \u0000 <p>β-Coronaviruses hijack host factors to remodel host endo-membranes to form double membrane vesicles (DMVs), which act as central hubs for the replication of viral genomes. Understanding the molecular mechanism underlying DMV formation is critical for developing effective antiviral strategies and has garnered significant attention. However, the host factors involved in DMV formation remain scanty. Here, we employed a bimolecular fluorescence complementation-based proximity labeling (BiFCPL) strategy to investigate the proteome of DMVs generated by co-expression of SARS-CoV-2 NSP3 and NSP4. Our analysis identified 62 proteins with high confidence, among which five proteins were localized in the endoplasmic reticulum (ER), and were further confirmed to be recruited to DMVs through interactions with NSP3/NSP4. Moreover, we demonstrated that the absence of GRAMD1B or TEX2 resulted in the formation of enlarged DMVs induced by either NSP3/NSP4 or coronaviruses infection, and impaired coronaviruses replication as well. Collectively, our study concerning host-virus interactions sheds light on novel host factors involved in DMV formation.</p></div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic Analysis of the Tonsil Virome Highlights Its Diagnostic Potential for Rheumatoid Arthritis","authors":"Xudong Gao,&nbsp;Ying Zheng,&nbsp;Huanhuan Chang,&nbsp;Jian Liu,&nbsp;Xiaoke Sun,&nbsp;Yi Ren,&nbsp;Lu Li,&nbsp;Gang Zhao,&nbsp;Yan Cheng,&nbsp;Shiyang Ma,&nbsp;Juhui Zhao,&nbsp;Jiang Deng,&nbsp;Tong Kang,&nbsp;Zhaojun Qiao,&nbsp;Hongli Zhao,&nbsp;Danhong Xie,&nbsp;Ting Wang,&nbsp;Shenghui Li,&nbsp;Haitao Shi,&nbsp;Ameng Shi,&nbsp;Pan Zhang","doi":"10.1002/jmv.70570","DOIUrl":"https://doi.org/10.1002/jmv.70570","url":null,"abstract":"<div>\u0000 \u0000 <p>Rheumatoid arthritis (RA) is a chronic autoimmune disease whose exact pathogenesis remains unclear, despite links to genetics, environmental factors, and microbial dysbiosis. Recent studies have highlighted the role of the microbiome in RA, yet the contribution of the tonsil virome remains unexplored. This study aims to investigate whether changes in the tonsil virome are associated with RA progression and assess its diagnostic potential. Using metagenomic data from 32 RA patients and 30 healthy controls (HCs), we identified 45 782 viral operational taxonomic units (vOTUs), with 14 341 classified as core vOTUs. RA patients exhibited significantly reduced virome richness and diversity, whereas Siphoviridae and Microviridae dominated both groups. Statistical analysis identified 235 RA-associated viral markers, including 13 enriched in RA and 222 in HCs. RA-enriched markers were primarily bacteriophages infecting Streptococcaceae, whereas HCs displayed more diverse viral–host interactions. Random forest models demonstrated strong discriminatory power of viral markers in distinguishing RA patients from HCs, achieving an AUC of 0.960, outperforming bacterial markers. Correlation analyses further linked viral markers to immune cell subsets, suggesting that tonsil virome alterations may influence immune dysregulation in RA. This study reveals significant changes in the tonsil virome of RA patients, highlighting its potential as a diagnostic tool and offering new insights into RA pathogenesis. These findings pave the way for future research into the virome's role in autoimmune diseases and therapeutic development.</p></div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does Lumpy Skin Disease Have the Potential to Become Zoonotic?","authors":"Lauro Velazquez-Salinas,&nbsp;Amanda M. Harvey,&nbsp;Chad Mire","doi":"10.1002/jmv.70553","DOIUrl":"https://doi.org/10.1002/jmv.70553","url":null,"abstract":"&lt;p&gt;The purpose of this letter is to call the attention of the scientific community to the recent manuscript published by Tomar and Khairnar [&lt;span&gt;1&lt;/span&gt;]. In this study, using a metagenomic analysis, the authors discovered the presence of genome fragments of Capripoxvirus lumpyskinpox (LSDV) in nasopharyngeal swab samples (obtained from SARS-CoV-2 surveillance activities during 2023) collected from 12 human subjects located in the districts of Nagpur, Chandrapur, and Bhandara state of Maharashtra, India. Herein, we present our perspective about the potential of LSDV to infect humans as well as our suggestions about further steps to confirm the results published by Tomar and Khairnar.&lt;/p&gt;&lt;p&gt;LSDV belongs to the poxvirus family and capripoxvirus genus. It produces lumpy skin disease—a highly contagious cattle-buffalo vector-borne disease reportable to the World Organization for Animal Health (WOAH) [&lt;span&gt;2&lt;/span&gt;]. Currently, none of the viral species included in this genus are considered zoonotic agents. The poxvirus family contains multiple viral species which can infect a variety of hosts (Figure 1). Currently, a total of eleven poxviruses linked to four poxviridae genera have demonstrated the ability to infect humans (Figure 1).&lt;/p&gt;&lt;p&gt;Although multiple poxviruses have the capability to infect humans, the most relevant poxviruses for public health are grouped in the orthopoxvirus genus (Figure 1A). These include orthopoxvirus variola (one of the deadliest viruses in human history) [&lt;span&gt;3&lt;/span&gt;] and orthopoxvirus monkeypox (the most relevant orthopoxvirus infection in humans in the orthopoxvirus variola post-eradication era) [&lt;span&gt;4&lt;/span&gt;]. These two viruses, along with the molluscum contagiosum [&lt;span&gt;5&lt;/span&gt;], are the only poxviruses with the ability to promote sustained human-to-human transmission. This ability is an important condition for a zoonotic virus that has crossed the species barrier, like orthopoxvirus monkeypox, to become significant for public health.&lt;/p&gt;&lt;p&gt;When considering the phylogenetic relationship of capripoxviruses with other poxviruses that have the ability to infect humans, capripoxviruses are most closely related to viruses in the genus Yatapoxvirus (Figure 1), which includes yatapoxvirus tanapox and yatapoxvirus yabapox. Neither of these viruses are regarded as a major human health threat [&lt;span&gt;6&lt;/span&gt;]; however, capripoxviruses and yatapoxviruses are linked to vector transmission [&lt;span&gt;7&lt;/span&gt;]. This characteristic, along with the capability of poxviruses to replicate in the cytoplasm, are two conditions positively associated with the potential zoonotic risk of a viral agent [&lt;span&gt;8&lt;/span&gt;].&lt;/p&gt;&lt;p&gt;Another important aspect of LSDV to consider is its potential to produce cross-species spillover. Between 2021 and 2023, six new animal species were discovered to be clinically infected with this virus [&lt;span&gt;9&lt;/span&gt;] (Figure 1A). Interestingly, half of these new hosts were detected in India during 2022–2023 [&lt;","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70553","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contribution of Hematological, Biochemical, and Inflammatory Factors in Temporal Variation of Disease Severity Among Eastern Indian Dengue Patients","authors":"Priyanka Ghosh,&nbsp;Sudeshna Mallik,&nbsp;Anusri Tripathi","doi":"10.1002/jmv.70567","DOIUrl":"https://doi.org/10.1002/jmv.70567","url":null,"abstract":"<div>\u0000 \u0000 <p>India, being dengue (DENV) endemic country, faces recurrent outbreaks. Favorable environmental conditions for dengue in eastern India also enhanced patient mortality during 2019–2024. The current study was performed to understand the effect of DENV secondary infection, hematological and biochemical parameters, serotypic shift, and viral load on disease severity. DENV was screened among 1169 symptomatic patients by qRT-PCR, NS1-antigen, anti-dengue-IgM, IgG-ELISA, and categorized according to WHO classification during 2020–2023. DENV-titer, serotype was determined by qRT-PCR. Statistical analysis was performed using GraphPad Prism 9 software. Among total 663 dengue + patients, 42.23%, 55.5%, 51.88%, 41.78% were confirmed by DENV-NS1-antigen, anti-dengue-IgM and IgG-antibody ELISA, qRT-PCR, respectively. Markedly increased prevalence of severe dengue symptoms, namely thrombocytopenia, hemorrhage, ascites, and liver enlargement, was observed during 2021–2022 and among secondary dengue patients. Severe dengue patients were mostly detected by IgM+, IgM+PCR+, NS1+IgG+PCR+, and NS1+IgM+IgG+PCR+ marker combinations. DENV3 and DENV2 + 3 serotypes were most prevalent during 2021–2022. Moreover, significantly decreased count of WBC, RBC, MCHC, platelet, albumin, HDL, LDL; increased amount of hemoglobin, HCT, ALT, ALP, urea, and AST/ALT was observed among patients of 2021–2022 and those with thrombocytopenia, hemorrhage, ascites and liver enlargement (<i>p</i> value &lt; 0.0001–0.0282). Significantly higher concentration of IL-6 and IL-8 among those with high viral load, secondary dengue infection, and disease severity also substantiated this finding (<i>p</i> value = 0.0104–0.0464). Thus, the present study provided evidence for the role of co-infecting serotypes, hematological, biochemical, and pro-inflammatory factors on year-wise complexity of dengue severity among eastern Indian patients.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dominance of DENV-1 and Flavivirus Serological Cross-Reactivity During the 2016 Dengue Outbreak in Vietnam","authors":"Do Duc Anh,&nbsp;Do Huy Loc,&nbsp;Hoang Van Tong,&nbsp;Do Thi Huyen Dieu,&nbsp;Ngo Thu Hang,&nbsp;Nguyen Huu Lanh,&nbsp;Nguyen Thi Hong Nhung,&nbsp;Peter G. Kremsner,&nbsp;Le Huu Song,&nbsp;Nguyen Linh Toan,&nbsp;Thirumalaisamy P. Velavan","doi":"10.1002/jmv.70569","DOIUrl":"https://doi.org/10.1002/jmv.70569","url":null,"abstract":"<p>Flaviviruses such as dengue virus (DENV), Zika virus (ZIKV), and Japanese encephalitis virus (JEV) pose a major health burden in Vietnam, where overlapping clinical features and serological cross-reactivity complicate accurate diagnosis and outbreak control. This study aimed to investigate circulating DENV serotypes and assess serological cross-reactivity with other flaviviruses during the 2016 dengue outbreak in central Vietnam. Aretrospective study was conducted on 146 hospitalized dengue patients during the 2016 outbreak in Binh Dinh province. Laboratory diagnosis included NS1antigen testing, ELISA (IgM/IgG), and real-time RT-PCR for DENV serotyping. IgM and IgG cross-reactivity with five flaviviruses, including DENV, ZIKV, JEV, West Nile virus (WNV), and tick-borne encephalitis virus (TBEV), and onealphavirus, chikungunya virus (CHIKV), was evaluated using ELISA. DENV-1 positive samples were further analysed by sequencing the capsid-premembrane (CprM) gene. DENV-1 was the predominant serotype (86%), with all sequenced strains clustering within genotype I. Secondary infections were more frequent (64%) than primary infections (36%) and were associated with a significantly higher median age (<i>p</i> = 0.003) and elevated hs-CRP levels (<i>p</i> = 0.029). Strong IgG cross-reactivity was observed among flaviviruses, particularly DENV, JEV, WNV, and TBEV (<i>r</i> &gt; 0.85), while ZIKV and CHIKV showed low seropositivity. Incontrast, IgM responses demonstrated greater virus specificity. Ten PCR-negative cases showed broad serological reactivity, suggesting possible misdiagnosis or late-stage infection. Our findings reveal that DENV-1 genotype I was the predominant serotype during the 2016 outbreak in central Vietnam. Extensive IgG cross-reactivity among flaviviruses hinders serological diagnosis, highlighting the need for integrated molecular surveillance to ensure accurate outbreak response.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70569","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to “RT-RPA Assisted CRISPR/Cas12a Based One-Pot Rapid and Visual Detection of the Pan-Dengue Virus”","authors":"","doi":"10.1002/jmv.70576","DOIUrl":"https://doi.org/10.1002/jmv.70576","url":null,"abstract":"<p>P. Bhardwaj, P. Dhangur, A. Kalichamy, and R. Singh, “RT-RPA Assisted CRISPR/Cas12a Based One-Pot Rapid and Visual Detection of the Pan-Dengue Virus,” <i>Journal of Medical Virology</i> <b>92</b>, no. 2: e70219, https://doi.org/10.1002/jmv.70219.</p><p>In the above-referenced article, typing errors occurred in the Abstract due to the authors' negligence. Corrections are provided below.</p><p>In Abstract, sentence 7 can be read as a fluorescence detection platform for Pan-DENV using RT-RPA and CRISPR/Cas12a was developed targeting nonstructural 1 (<i>NS1</i>) gene for <b>DENV-1, 3, and 4</b> instead of <b>DENV-1, 2, and 3</b>.</p><p>And sentence 13 can be read as Our assay demonstrated the analytic sensitivity of ≥ 10 ng reaction⁻¹ for DENV-1 and DENV-4, and ≥ 0.5 ng reaction⁻¹ for <b>DENV-2 and DENV-3 genomes</b> instead of <b>DENV-3 and DENV-4 genomes.</b></p><p>The authors regret these errors.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70576","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of NTRK Fusion Genes in the Tumor Immune Microenvironment of HPV (+/−) Cervical Cancer","authors":"Qiongying Wang,&nbsp;Chan Zhang,&nbsp;Shijia Liu,&nbsp;Wangshu Li,&nbsp;Wenjuan Wei,&nbsp;Aziz ur Rehman Aziz,&nbsp;Han Lu,&nbsp;Daqing Wang","doi":"10.1002/jmv.70564","DOIUrl":"https://doi.org/10.1002/jmv.70564","url":null,"abstract":"<p>Cervical cancer, a prevalent gynecological malignancy, is primarily associated with human papillomavirus (HPV) infection. However, some cases display distinct molecular alterations beyond HPV, significantly impacting the tumor immune microenvironment (TIME) and posing therapeutic challenges. Among these molecular changes, NTRK gene fusions have emerged as critical drivers of tumor progression, invasiveness, and poor prognosis. This review highlights the role of NTRK fusions in cervical cancer oncogenesis, examining their effects on cellular signaling pathways and their interplay with HPV status in shaping TIME. The relationship between HPV infection and NTRK fusions is explored, revealing their combined influence on disease progression. Additionally, the potential of NTRK-targeted therapies in precision oncology is discussed, emphasizing their promise as treatment options for cervical cancer. By addressing the molecular, immune, and clinical complexities of cervical cancer, this review provides valuable insights into advancing research and therapeutic strategies.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70564","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of Amplicon-Based Protocol for Sequencing of Respiratory Syncytial Virus Genome","authors":"Guglielmo Ferrari,&nbsp;Great Romano,&nbsp;Antonino Maria Guglielmo Pitrolo,&nbsp;Fausto Baldanti,&nbsp;Antonio Piralla","doi":"10.1002/jmv.70571","DOIUrl":"https://doi.org/10.1002/jmv.70571","url":null,"abstract":"<p>The most prevalent cause of severe respiratory infections in children is the human respiratory syncytial virus (RSV). The advent of next-generation sequencing (NGS) has made it possible to incorporate this technology into pathogen monitoring and surveillance. Whole-genome sequencing (WGS) of RSV has now become a relatively widely used method for tracking viral evolution. Here we report an improved high-throughput RSV-WGS assay performed directly on clinical samples that is suitable for short-read sequencing platforms. A total of 100 RSV-positive samples collected between November 2022 and March 2024 fulfilled the inclusion cycle quantification criteria and were randomly included in the validation process. The WGS protocol was designed to amplify three distinct amplicons to cover the entire RSV genome. The protocol described here can be successfully replicated in several instances (approximately 95%) in samples with a relatively low viral load, typically corresponding to cycle of quantification values of 27–32. The amplicon-based protocol produced meaningful sequencing results in terms of median depth of coverage (more than 12000×) and median of mapped reads (&gt; 1 × 10⁶ reads). The sequences that had passed the filters showed a coverage of at least 98% across the entire genome, with cycle quantification values of 32. Based on the obtained data resulting in an easy-to-perform protocol helpful for the molecular epidemiology surveillance of RSV.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70571","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High Cytomegalovirus Load Is Associated With More Severe CMV-Associated Thrombocytopenia: A 6-Year Study in Eastern China","authors":"Qiji Guo,&nbsp;Yuhang Wu,&nbsp;Hao Xu,&nbsp;Xiaohui Fang,&nbsp;Xiaojun Xu,&nbsp;Ao Dong,&nbsp;Hongqiang Shen,&nbsp;Wei Li,&nbsp;Tianming Yuan,&nbsp;Qiang Shu,&nbsp;Lisu Huang","doi":"10.1002/jmv.70568","DOIUrl":"https://doi.org/10.1002/jmv.70568","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>Cytomegalovirus (CMV)-associated thrombocytopenia is a rare complication, and its prevalence, severity, and prognosis remain poorly characterized. Among 2927 children confirmed CMV infection, 87 (3%) had thrombocytopenia from July 2018 to April 2024. The clinical characteristics of CMV-associated thrombocytopenia were described, according to CMV-DNA load. Logistic regression was used to compare the risk factors for organ hemorrhage. In this prospective cohort of CMV-associated thrombocytopenia, most children were infants with onset age at 53 (24.0–90.3) days. Among them, 78.2% exhibited bleeding manifestations, 16.1% experienced organ hemorrhage, 51.7% had severe thrombocytopenia, and 5.7% died. Interestingly, low platelet count (≤ 20 × 10⁹/L) was not a high-risk factor (OR, 3.89 [95% CI, 0.59–42.37]) for organ hemorrhage. However, patients in the high CMV-DNA load group had a 3.61 times higher risk of organ hemorrhage (OR, 3.61 [95% CI, 1.03–12.65]), which remained significant in subgroup analyses according to shorter activated partial thromboplastin time (OR, 5.16 [95% CI, 1.16–22.89]). In conclusion, CMV infection may lead to significant thrombocytopenia, which result in severe organ hemorrhage particularly in infants. High CMV-DNA load contributes to the severity of the condition.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viral Reprogramming of Nucleotide Synthesis and Its Impact on Viral Infection","authors":"Lara Dsouza,&nbsp;Zhilong Yang","doi":"10.1002/jmv.70563","DOIUrl":"https://doi.org/10.1002/jmv.70563","url":null,"abstract":"<p>Nucleotides are the building blocks of DNA and RNA. They also play essential roles in various other biological processes, including protein glycosylation, ribosome biogenesis, and cytoskeletal function. The significance of the regulation of nucleotide metabolism has recently gained more attention in many physiological and pathological contexts, including viral infections that often reprogram host cell metabolism to support viral replication. However, whilst nucleotides have long been known to be important for viral nucleic acid synthesis, the molecular mechanisms undertaken by viruses to regulate nucleotide synthesis are only beginning to be understood. In this review, we present a comprehensive analysis of nucleotide regulation by upstream growth factor signaling mechanisms in various families of RNA and DNA viruses, such as herpesviruses, poxviruses, influenza viruses, and coronaviruses. We place a primary emphasis on discussing the signaling pathways as the regulatory mechanisms and highlight the gaps in understanding the mechanistic details. We underscore recent research that investigates the roles of different viral factors in modulating nucleotide metabolism in the infections of DNA and RNA viruses. Finally, we discuss the emerging area of inquiry that explores the relationship between nucleotide metabolism and immune regulation. A thorough understanding of how nucleotides are regulated during viral infections is essential for developing novel effective therapeutic strategies against these viruses.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70563","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}