Journal of Medical Virology最新文献

{"title":"Differential Serotype Specificity in the IgG Subclass Profile of the Anti-Domain III Response Elicited by Dengue Virus Infection","authors":"Luis Gabriel González-Lodeiro,&nbsp;Patricia Barrios Roque,&nbsp;Nivaldo Gómez Hernández,&nbsp;Danya Medina-Carrasco,&nbsp;Lisandra E. García de Castro,&nbsp;Vivian Huerta Galindo","doi":"10.1002/jmv.70255","DOIUrl":"https://doi.org/10.1002/jmv.70255","url":null,"abstract":"<div>\u0000 \u0000 <p>Dengue is a potentially fatal disease caused by any of the four serotypes of dengue virus complex (DENV1-4). Domain III (DIII) of the envelope protein mediates early virus:cell interactions and is target of potent neutralizing antibodies. Little data is available on the dynamic of IgG subclasses in anti-DIII response elicited during viral infection. Fifty-eight human sera were used to characterize the IgG subclass profile of the anti-DIII antibody response in terms of abundance and serotype-specificity. Immunodominant epitopes were also determined using 70 Ala-mutants of a recombinant DIII protein that spans residues with more than 15% of the exposed area in the virion. IgG1 and IgG3 were found as the subclasses that react to control primary infections while a significant response was detected for all IgG subclasses in response to secondary infections. Anti-DIII IgG1 exhibits a distinctive pattern of serotype-specificity with respect to the other IgG subclasses in the recognition of recombinant DIII proteins corresponding to the four DENV serotypes. The dominant epitope of IgG1 is located in the FG-loop, which is characterized by high variability in its amino acid sequence. In contrast, the dominant epitopes of IgG2, IgG3, and IgG4 were defined as regions enriched in complex- and subcomplex conserved residues such as the A-strand and the AB-loop of DIII. IgG1 plays a prominent role in neutralizing circulating DENV during infection. A balanced and timely response of the different IgG subclasses is critical in the evolution of dengue disease.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 3","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Adenovirus B14p1 First Identification in Conjunctival Swabs From a Patient With Moderately Acute Follicular Conjunctivitis in Japan","authors":"Nozomu Hanaoka,&nbsp;Hisatoshi Kaneko,&nbsp;Noriko Yaguchi,&nbsp;Kenichiro Takahashi,&nbsp;Kosuke Murakami","doi":"10.1002/jmv.70265","DOIUrl":"https://doi.org/10.1002/jmv.70265","url":null,"abstract":"","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 3","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reinfection With Cytomegalovirus During Pregnancy: A Prospective Cohort Study in Canada","authors":"Juliette St-Georges,&nbsp;Safari Joseph Balegamire,&nbsp;Ariane Larouche,&nbsp;Suresh Boppana,&nbsp;Christian Renaud,&nbsp;Benoît Mâsse,&nbsp;Yves Giguere,&nbsp;Jean-Claude Forest,&nbsp;Valerie Lamarre,&nbsp;François Audibert,&nbsp;Soren Gantt,&nbsp;Isabelle Boucoiran","doi":"10.1002/jmv.70261","DOIUrl":"https://doi.org/10.1002/jmv.70261","url":null,"abstract":"<p>Congenital cytomegalovirus infections (cCMV) are an important cause of childhood neurodevelopmental deficits. Most cCMV are the result of maternal non-primary infections during pregnancy, which can be due to reactivation or reinfection. To identify the rate of CMV reinfection during pregnancy and its risk factors. We performed a secondary analysis of CMV seropositive participants from two prospective cohort studies in Quebec, Canada. Antibody responses to four strain-specific CMV epitopes located in glycoproteins B and H were measured by enzyme-linked immunosorbent assay. CMV reinfection was defined as the appearance of an antibody response to a new epitope in the third compared to the first trimester. Risk factors for reinfection were assessed. Among 1614 participants, CMV reinfection was identified in 2.7% of participants, representing an incidence of 54.99 per 1000 person-years at risk (95% confidence interval 39.95–73.82). Age, marital status, household income, continent of birth or ethnicity were not associated with reinfection during pregnancy. The incidence of CMV reinfection during pregnancy is like what has been reported for primary infection in Quebec. A greater understanding of the patterns of reinfection is needed to inform strategies to reduce the burden of disease from cCMV.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 3","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70261","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic and Genetic Evolution Analysis of Complete SFTSV Genome Sequences in Shandong Province, China","authors":"Shixiao Dong,&nbsp;Haitao Jiao,&nbsp;Hong Zhao,&nbsp;Lili Wang,&nbsp;Xun Peng,&nbsp;Li Song,&nbsp;Lintao Sai","doi":"10.1002/jmv.70263","DOIUrl":"https://doi.org/10.1002/jmv.70263","url":null,"abstract":"<div>\u0000 \u0000 <p>Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by SFTS virus (SFTSV). Shandong province is one of the epidemic regions with high incidence rate of SFTS. To investigate phylogenetical and genetic evolution characteristics of SFTSV in Shandong province, we isolated SFTSV from suspected patients between April 2023 and October 2024, and then whole SFTSV genomes were amplified and sequenced in this study. A total of 25 new strains were analyzed together 56 strains submitted in Genbank from Shandong province. Phylogenetical and genetic analyses of the data set revealed that four genotypes were co-circulating in Shandong province. C3 genotype was the most common genotype in each year with lower genetic divergence. 298 amino acid substitutions were detected in the four proteins of SFTSV, but only two substitutions (Arg624Lys and Arg962Ser) had been proven to have potential impacts on biological functions. In addition, one reassortment strain (C3/C4/C4 for L, M and S segments) and three recombinant strains were identified. Analysis of selection pressure at the level of amino acid substitutions indicated genes within the four ORFs of SFTSV were all subjected to negative selection. In conclusion, the genetic characteristics and evolutionary mechanism of SFTSV was complex in Shandong province. It is necessary to conduct continuous surveillance to grasp the genetic evolution patterns, and to discover novel prevalent variants in a timely manner.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 3","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HERV Modulation in Colorectal Carcinoma Patients: A Snapshot of Endogenous Retroviral Transcriptome","authors":"Nicole Grandi,&nbsp;Ching-Hsuan Liu,&nbsp;Saili Chabukswar,&nbsp;Daniele Carta,&nbsp;Yun Yen,&nbsp;Liang-Tzung Lin,&nbsp;Enzo Tramontano","doi":"10.1002/jmv.70249","DOIUrl":"https://doi.org/10.1002/jmv.70249","url":null,"abstract":"<p>Human endogenous retroviruses (HERVs) are proviral relics of infections that affected primates' germ line. Many HERV elements retain a residual capacity to encode transcripts and proteins that have been occasionally domesticated for the host physiology. In addition, HERV transcriptional modulation is of great interest to clarify the etiology of complex disorders such as cancer, even if a few studies assessed the specific HERV loci modulated in tumor tissues. In the present work, we used a transcriptomic approach to investigate the specific expression of ~3300 HERV loci in paired tumor and normal tissues of 7 colorectal cancer (CRC) patients. A total of 102 HERVs were significantly modulated in CRC, with a general tendency towards downregulation. Of note, among the 42 upregulated HERVs 23 belonged to the HERV-H group, that is the most investigated in CRC. De novo transcriptome reconstruction and qPCR validation allowed to identify a transcript from a HERV-H locus on chromosome Xp22.3 with high specific expression in CRC samples, potentially encoding for a partial Pol protein. These results provide a detailed description of HERV transcriptional variations in CRC and its interindividual variability, identifying a HERV-H transcript that deserves further investigation for its possible impact on tumor progression.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 3","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70249","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover Image, Volume 97, Number 2, February 2025","authors":"Huanhuan Lu,&nbsp;Jinbo Xiao,&nbsp;Jingdong Song,&nbsp;Yang Song,&nbsp;Hai Li,&nbsp;Hu Ren,&nbsp;Jichen Li,&nbsp;Ruyi Cong,&nbsp;Hangwen Li,&nbsp;Yi Fang,&nbsp;Dongmei Yan,&nbsp;Shuangli Zhu,&nbsp;Qiang Sun,&nbsp;Ying Liu,&nbsp;Yong Zhang","doi":"10.1002/jmv.70270","DOIUrl":"https://doi.org/10.1002/jmv.70270","url":null,"abstract":"<p><b>Front Cover Caption:</b> The cover image is based on the article <i>The Immunogenicity of Coxsackievirus A6 (D3a Sub-Genotype) Virus-Like Particle and mRNA Vaccines</i> by Yong Zhang et al., https://doi.org/10.1002/jmv.70201.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70270","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143471976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urgent Focus on the Surgical Risks of People Living With HIV: A Systematic Review and Meta-Analysis","authors":"Kangpeng Li,&nbsp;Lingxue Luo,&nbsp;Yunxiao Ji,&nbsp;Qiang Zhang","doi":"10.1002/jmv.70260","DOIUrl":"https://doi.org/10.1002/jmv.70260","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>With the widespread promotion and application of antiretroviral therapy in clinical practice, people living with HIV (PLWH) have the potential to live as long as non-HIV people and the probability of surgery for PLWH has been increasing dramatically. However, the overall postoperative outcome and risk are still unclear. We performed comprehensive and methodical searches in PubMed, Embase, and Web of Science without date and language restrictions. Study outcomes included: (1) cure rate, (2) mortality, (3) reoperation rate, (4) incidence of any postoperative complications, (5) length of stay, and (6) operation duration. NOS scores were employed to evaluate bias risk, while publication bias was assessed using funnel plots and Egger tests. Review Manager version 5.4.1, R version 4.4.1, and Stata version 14.0 were employed to determine quantitative analysis, considering a significance level of <i>p </i>&lt; 0.05. A total of 50 studies were included, involving 54 565 PLWH undergoing surgical treatment. Synthesis analysis showed that the mortality (OR = 1.70, 95% CI: 1.58−1.83, <i>p </i>&lt; 0.00001), reoperation rate (OR = 1.78, 95% CI: 1.36−2.34, <i>p </i>&lt; 0.00001), complication rate (OR = 1.56, 95% CI: 1.26−1.95, <i>p </i>&lt; 0.00001), LOS (OR = 1.63, 95% CI: 1.28−1.99, <i>p </i>&lt; 0.00001), and operation time (OR = 7.37, 95% CI: 1.14−13.59, <i>p </i>= 0.02) were increased in PLWH. However, there was no significant difference in the cure rate compared to the control group (OR = 1.27, 95% CI: 0.90−1.79, <i>p </i>= 0.18). Subgroup analysis showed that complication rates increased again in orthopedic (OR = 1.65, 95% CI: 1.34−2.05, <i>p </i>&lt; 0.00001) and general surgery (OR = 1.72, 95% CI: 1.08−2.74, <i>p </i>= 0.02). However, the type of procedure, publication quality, study type, and patient origin were not sources of complication rate heterogeneity. Meta-regression showed that CD4 count had no effect on complication rate, but the anti-retroviral therapy rate had 34.89% explanatory power. There is an increased risk of postoperative death, reoperation, complications, and prolonged hospital stay and surgical duration in PLWH. However, conducting extensive prospective studies across multiple centers is crucial to validate these findings.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and Analytical Evaluation of the Abbott Alinity m HR HPV Assay in a New Generation First-Void Urine Collector","authors":"Severien Van Keer,&nbsp;Laura Téblick,&nbsp;Gilbert Donders,&nbsp;Steven Weyers,&nbsp;Jean Doyen,&nbsp;Ann Cornelis,&nbsp;Koen Van de Vijver,&nbsp;Katty Delbecque,&nbsp;Annemie De Smet,&nbsp;Pierre Van Damme,&nbsp;Alex Vorsters","doi":"10.1002/jmv.70254","DOIUrl":"https://doi.org/10.1002/jmv.70254","url":null,"abstract":"<div>\u0000 \u0000 <p>Urine-based self-sampling approaches can simplify cervical screening programs whilst increasing response. This study reports on the performance of Abbott Alinity m HR HPV on urine, self-collected at home using a new generation first-void urination device that is suitable for postal delivery (Novosanis Colli-Pee Small Volumes). First-void urine and paired cervical samples from 297 females attending colposcopy (age 25–65, NCT04530201) were analysed for the presence of Human Papillomavirus (HPV) DNA. Cervical disease was confirmed by colposcopy and/or histology. HPV testing on first-void urine was less sensitive for high-grade cervical intraepithelial neoplasia (CIN2 +; ratio 0.91; 95% CI: 0.83–0.99), though equally specific (&lt; CIN2; ratio 1.04; 95% CI: 0.92–1.19) compared to cervical samples at the manufacturer established cut-off for cervical samples. Adjusting the cut-off for first-void urine improved sensitivity for CIN2+ (ratio 0.96; 95% CI: 0.90–1.03), whilst maintaining equal specificity compared to cervical samples (ratio 1.00; 95% CI: 0.88–1.14). Cohen's kappa agreements of HPV outcomes between sample pairs were good to excellent at both cut-offs (range: 0.64–0.85). Using the HPV test's adjusted cutoff for first-void urine, no difference in clinical sensitivity or specificity was observed between first-void urine and cervical samples. These data highlight the importance of evaluating self-sample-specific cut-offs for HPV assays, previously validated on cervical samples.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Animal Models for Long COVID: Current Advances, Limitations, and Future Directions","authors":"Yu Zhang,&nbsp;Huan Chen,&nbsp;Yumeng Li,&nbsp;Chenxi Luo,&nbsp;Yunkai Zhu,&nbsp;Xiaoyang Zhou,&nbsp;Ruixuan Wang,&nbsp;Jiuxiang He,&nbsp;Hongxia Guo,&nbsp;Xiaofeng Xu,&nbsp;Minyue Qiu,&nbsp;Jintao Li","doi":"10.1002/jmv.70237","DOIUrl":"https://doi.org/10.1002/jmv.70237","url":null,"abstract":"<div>\u0000 \u0000 <p>Long COVID (LC) represents a chronic, systemic, and often disabling condition that poses a significant ongoing threat to public health. Foundational scientific studies are needed to unravel the underlying mechanisms, with the ultimate goal of developing effective preventative and therapeutic strategies. Therefore, there is an urgent demand for animal models that can accurately replicate the clinical features of LC. This review integrates clinical epidemiological data to summarize the pathological changes in extrapulmonary systems involved in LC. Additionally, it critically examines the capacity of existing animal models, including nonhuman primates, genetically modified mice, and Syrian hamsters, to exhibit enduring postinfection symptoms that align with human clinical manifestations, and identifies key areas requiring further development. The objective is to offer insights that will aid in the development of next-generation animal models, thereby accelerating our understanding of how acute respiratory viral infections transition into chronic conditions, and ensuring preparedness for future pandemics.</p></div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Evaluation of the PowerChek Respiratory Virus Panel RT-PCR Assay Detecting 16 Respiratory Viruses Including SARS-CoV-2","authors":"Jinyeong Kim,&nbsp;Eunhee Han,&nbsp;Jieun Kim,&nbsp;Young Jin Kim,&nbsp;Mi Hyun Bae","doi":"10.1002/jmv.70243","DOIUrl":"https://doi.org/10.1002/jmv.70243","url":null,"abstract":"<p>The endemic transition of coronavirus disease 2019 (COVID-19) has made diagnosis of acute respiratory infections complex. There is a demand for tests that can detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with other respiratory viruses. The PowerChek Respiratory Virus Panel (PowerChek, Kogenebiotech) is a newly developed real-time reverse transcription polymerase chain reaction (RT-PCR) assay that simultaneously detects SARS-CoV-2 and 15 other respiratory viruses; however, its performance requires assessment. The aim of this study was to compare the performance of the PowerChek assay with that of established RT-PCR assays. Nasopharyngeal swab specimens were obtained from patients with acute respiratory symptoms. Specimens were tested using the Allplex Respiratory Virus panel, Allplex SARS-CoV-2 PCR assays (Allplex, Seegene), and PowerChek assay. In total, 407 specimens were tested. The PowerChek assay detected all positive cases identified by the Allplex assays and 11 additional detection targets in 10 specimens. The overall agreement between the two assays was 97.5% (397/407). The PowerChek Respiratory Virus Panel assay demonstrated a reliable performance comparable to that of established RT-PCR assays. This assay could be clinically useful because the simultaneous detection of SARS-CoV-2 and other respiratory viruses and pre-dispensed RT-PCR reagents can simplify testing and diagnosis.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}