Journal of Medical Virology最新文献_第8页

A Single-Cell Atlas Revealed Altered B Cells and Neutrophils Immune Signatures and Inflammatory Responses in SFTSV Infection 单细胞图谱揭示了SFTSV感染中B细胞和中性粒细胞免疫特征和炎症反应的改变

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2025-04-22 DOI: 10.1002/jmv.70354

Qiujing Wang, Ziniu Dai, Xiaodan Hu, Zhengmei Lu, Di Zheng, Lingyun Wang, Liyun Xu, Xiaoci Hong, Jinhao Bi, Xinyi Li, Dapeng Li, Shibo Li

{"title":"A Single-Cell Atlas Revealed Altered B Cells and Neutrophils Immune Signatures and Inflammatory Responses in SFTSV Infection","authors":"Qiujing Wang, Ziniu Dai, Xiaodan Hu, Zhengmei Lu, Di Zheng, Lingyun Wang, Liyun Xu, Xiaoci Hong, Jinhao Bi, Xinyi Li, Dapeng Li, Shibo Li","doi":"10.1002/jmv.70354","DOIUrl":"https://doi.org/10.1002/jmv.70354","url":null,"abstract":"<div>\u0000 \u0000 <p>Severe fever with thrombocytopenia syndrome virus (SFTSV) presents an emerging public threat due to its high mortality rate and ever-expanding geographic distribution. However, characterization of SFTSV infection pathogenesis and immunological impact at single-cell level remains underexplored. Here, we employ single-cell transcriptome-wide sequencing in peripheral blood mononuclear cells (PBMCs) from hospitalized SFTSV-infected patients to map the immune landscape across acute and convalescent stages of infection. The results reveal significant alterations in immune cell compositions, along with profound disruption in intercellular crosstalk. B cells and neutrophils appear to be the primary target for SFTSV infection besides monocytes, as evidenced by heightened virus-related pathways in these two cell types during the acute phase. In addition, SFTSV infection induces a substantial inflammatory response, which were prominently reflected in monocytes and neutrophils. These data illustrate the complex immune remodeling and inflammatory cascades triggered by SFTSV, with a particular focus on its effects on B cells and neutrophils, bringing novel insights into future therapeutic developments.</p></div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 5","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Cell Atlas of the Peripheral Immune Response in Patients With Chronic Hepatitis B 慢性乙型肝炎患者外周血免疫应答的单细胞图谱

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2025-04-21 DOI: 10.1002/jmv.70360

Li Huang, Bo Ye, Feinan Cao, Bing Ruan, Xuefen Li

{"title":"Single-Cell Atlas of the Peripheral Immune Response in Patients With Chronic Hepatitis B","authors":"Li Huang, Bo Ye, Feinan Cao, Bing Ruan, Xuefen Li","doi":"10.1002/jmv.70360","DOIUrl":"https://doi.org/10.1002/jmv.70360","url":null,"abstract":"<div>\u0000 \u0000 <p>Cellular immune responses are crucial in determining outcomes of the hepatitis B virus (HBV) infection. Ineffective immune responses enable persistent HBV infection and contribute to progressive liver disease. Understanding the mechanisms underlying immunological HBV tolerance and restoring functional adaptive immune responses is essential for successful chronic hepatitis B (CHB) treatment. This study examined the dysregulated immune responses and immunopathological cell states associated with CHB using single-cell RNA sequencing of peripheral blood mononuclear cells to investigate immune cell composition and transcriptional differences between patients with CHB and healthy donors. Phenotypic alterations in the lymphoid and myeloid compartments were observed following HBV infection. T cell immune profiling in patients with CHB showed enrichment of exhausted CD8+ T cells, impaired cytotoxicity of effector CD8+ T cells, and increased regulatory T cell (Treg) suppressive activity. Immature neutrophils and a unique CD14+ monocyte subset (myeloid-derived suppressor cells) exhibited potent immunosuppressive abilities. A novel population of CD14+CD163+VSIG4+ M2-like macrophages with immunosuppressive and anti-inflammatory phenotypes was enriched in a patient with severe CHB and liver failure, indicating a potential contribution to dysfunctional immune responses. Our study demonstrated immune exhaustion and evasion in chronic HBV infection, elucidating its immunopathological features and suggesting new therapeutic strategies for immune-mediated disorders and unresolved chronic HBV infection.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 5","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Visual Nomogram Survival Prediction Model in Acquired Immune Deficiency Syndrome (AIDS)-Related Diffuse Large B-Cell Lymphoma 获得性免疫缺陷综合征（AIDS）相关弥漫大 B 细胞淋巴瘤的可视化提名图生存预测模型

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2025-04-21 DOI: 10.1002/jmv.70359

Tao Yang, Haike Lei, Jun Li, Yang Liang, Chaoyu Wang, Jun Liu, Yan Wu, Jun Liu, Qiwen Zhou, Haiyan Min, Zailin Yang, Xiaomei Zhang, Yunhong Huang, Guo Wei, Wei Zhang, Min Wang, Xiaoqiong Tang, Zhanshu Liu, Yaokai Chen, Hui Zhou, Robert Peter Gale, Yongzhong Wu, Yao Liu

{"title":"A Visual Nomogram Survival Prediction Model in Acquired Immune Deficiency Syndrome (AIDS)-Related Diffuse Large B-Cell Lymphoma","authors":"Tao Yang, Haike Lei, Jun Li, Yang Liang, Chaoyu Wang, Jun Liu, Yan Wu, Jun Liu, Qiwen Zhou, Haiyan Min, Zailin Yang, Xiaomei Zhang, Yunhong Huang, Guo Wei, Wei Zhang, Min Wang, Xiaoqiong Tang, Zhanshu Liu, Yaokai Chen, Hui Zhou, Robert Peter Gale, Yongzhong Wu, Yao Liu","doi":"10.1002/jmv.70359","DOIUrl":"https://doi.org/10.1002/jmv.70359","url":null,"abstract":"<div>\u0000 \u0000 <p>Estimating the prognosis of people with newly diagnosed AIDS-related diffuse large B-cell lymphoma (AR-DLBCL) is challenging. We did a prospective, multicenter cohort study using data from 306 consecutive subjects, including training cohort (<i>N</i> = 215) and external validation cohorts (<i>N</i> = 91), to develop and validate a visual nomogram, termed the ARDPI model. Seven co-variates were independently correlated with survival, including age, LMR, CD5, blood EBV-DNA copy number, CD4/CD8, CNS involvement, and anti-HIV therapy (ART), were used to develop the ARDPI model. AUROCs of the model for 1-, 3-, and 5-year survivals were 0.80 (95% CI: 0.72, 0.88), 0.78 (0.69, 0.87), and 0.77 (0.63, 0.91) in the training cohort and 0.85 (0.75, 0.95), 0.80 (0.66, 0.94), and 0.79 (0.61, 0.99) in the external validation cohort. The prediction accuracy of the ARDPI model was better compared with the IPI and NCCN-IPI models. Using the ARDPI model, we identified three risk cohorts with 3-year survivals of 88% (79, 98%), 35% (23, 54%), and 23% (12, 45%) in the training cohort (<i>p</i> < 0.001) and 93% (80, 100%), 46% (27, 78%), and 17% (5, 47%) in the external validation cohort (<i>p</i> < 0.001). The ARDPI accurately predicts the survival of newly diagnosed persons with AR-DLBCL and has clinical benefits. Accuracy is better compared with the IPI and NCCN-IPI prediction models. We also developed a web server to facilitate using our model.</p></div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 5","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Post-Acute Sequelae of COVID-19 on Irritable Bowel Syndrome in Individuals With Mental Illness in South Korea: A Population-Based Cohort Study COVID-19对韩国精神疾病患者肠易激综合征急性后后遗症：一项基于人群的队列研究

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2025-04-19 DOI: 10.1002/jmv.70345

Tae Hyeon Kim, Yejun Son, Jaeyu Park, Soeun Kim, Hyesu Jo, Hayeon Lee, Dong Keon Yon

引用次数: 0

Analysis of Blood Microbiome From People Living With HIV and Donors by 16S rRNA Metagenomic Sequencing 用16S rRNA宏基因组测序分析HIV感染者和献血者血液微生物组

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2025-04-18 DOI: 10.1002/jmv.70341

Qianqian Wei, Limin Chen, Yijing Yin, Mudao Pai, Hongmei Duan, Wenlan Zeng, Xue Hu, Min Xu, Shilin Li

{"title":"Analysis of Blood Microbiome From People Living With HIV and Donors by 16S rRNA Metagenomic Sequencing","authors":"Qianqian Wei, Limin Chen, Yijing Yin, Mudao Pai, Hongmei Duan, Wenlan Zeng, Xue Hu, Min Xu, Shilin Li","doi":"10.1002/jmv.70341","DOIUrl":"https://doi.org/10.1002/jmv.70341","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>Utilize 16S rRNA sequencing technology to characterize bacterial species susceptible to people living with HIV (PLWH) across different stages. This mapping aims to establish a foundational framework for preventing secondary HIV infections, prolonging patient survival, enhancing quality of life, and advancing the diagnosis, treatment, and research of bacterial co-infections. In this study, we classified the participants into three groups: The blood of donors living with HIV (DI group), AIDS patients who have received ART treatment (PI group), and healthy blood donors as the control group (DH group). Each group was divided into three parallel subgroups, with 30 samples pooled from each parallel group for plasma extraction. As initial processing steps, the nine parallel subgroups were subjected to nucleic acid extraction and PCR amplification targeting the 16SV34 region. The resulting amplified products were subsequently forwarded to a sequencing company. It can be seen from the Venn diagram that the DI groups showed significantly higher bacterial diversity than the PI group and the DH group. The PI group had lower bacterial relative abundance and diversity compared to the DI group, with a community structure more similar to the control group. The DI group is particularly susceptible to several significant pathogens, including Ralstonia, Pseudomonas, Acinetobacter, Methyloversatilis, and Vibrio. The study revealed a greater quantity and diversity of bacteria in the DI blood compared to the PI and DH groups. This observation may be attributed to PI group patients in this study being hospitalized and receiving treatment.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 4","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enrichment of G-to-U Substitution in SARS-CoV-2 Functional Regions and Its Compensation via Concurrent Mutations SARS-CoV-2功能区域G-to-U取代的富集及其通过并发突变的补偿

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2025-04-18 DOI: 10.1002/jmv.70353

Xierzhatijiang Sulaiman, Yan Han, Sheng Liu, Kailing Li, Marissa Shang, Lei Yang, Kenneth White, Yong Zang, Jikui Shen, Jun Wan

{"title":"Enrichment of G-to-U Substitution in SARS-CoV-2 Functional Regions and Its Compensation via Concurrent Mutations","authors":"Xierzhatijiang Sulaiman, Yan Han, Sheng Liu, Kailing Li, Marissa Shang, Lei Yang, Kenneth White, Yong Zang, Jikui Shen, Jun Wan","doi":"10.1002/jmv.70353","DOIUrl":"https://doi.org/10.1002/jmv.70353","url":null,"abstract":"<p>We surveyed single nucleotide variant (SNV) patterns from 5 903 647 complete SARS-CoV-2 genomes. Among 10 012 SNVs, APOBEC-mediated C-to-U (C > U) deamination was the most prevalent, followed by G > U and other RNA editing-related substitutions including (A > G, U > C, G > A). However, C > U mutations were less frequent in functional regions, for example, S protein, intrinsic disordered regions, and nonsynonymous mutations, where G > U were over-represented. Notably, G-loss substitutions rarely appeared together. Instead, G-gain mutations tended to more frequently co-occur with others, with a marked preference in the S protein, suggesting a compensatory mechanism for G loss in G > U mutations. The temporal patterns revealed C > U frequency declined until late 2021 then resurged in early 2022. Conversely, G > U steadily decreased, with a pronounced drop in January 2022, coinciding with reduced COVID-19 severity. Vaccinated individuals exhibited a slightly but significantly higher C > U frequency and a notably lower G > U frequency compared to the unvaccinated group. Additionally, cancer patients had higher G > U frequency than general patients during the same period. Interestingly, none of the C > U SNVs were uniquely identified in 2724 environmental samples. These findings suggest novel functional roles of G > U in COVID-19 symptoms, potentially linked to oxidative stress and reactive oxygen species, while C > U remains the dominant substitution, likely driven by host immune-mediated RNA editing.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 4","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70353","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Phase I Study Evaluating Safety and Tolerability of Viral-Specific T Cells Against BK-Virus in Adult Kidney Transplant Recipients 一项评估成人肾移植受者抗bk病毒病毒特异性T细胞安全性和耐受性的I期研究

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2025-04-18 DOI: 10.1002/jmv.70357

Lucy Ptak, Ross O. Meyers, Olga Radko-Ganz, Kimberly A. McDowell, Margaret Jorgenson, Maggie Chilsen, Didier Mandelbrot, Jacques Galipeau, Sandesh Parajuli

{"title":"A Phase I Study Evaluating Safety and Tolerability of Viral-Specific T Cells Against BK-Virus in Adult Kidney Transplant Recipients","authors":"Lucy Ptak, Ross O. Meyers, Olga Radko-Ganz, Kimberly A. McDowell, Margaret Jorgenson, Maggie Chilsen, Didier Mandelbrot, Jacques Galipeau, Sandesh Parajuli","doi":"10.1002/jmv.70357","DOIUrl":"https://doi.org/10.1002/jmv.70357","url":null,"abstract":"<p>BK polyomavirus (BKPyV) poses a significant threat to kidney transplant recipients (KTR). Current management primarily involves reducing immunosuppression, which increases the risk of rejection. Cell-based immunotherapy with virus-specific T cells (VST) has emerged as an alternative approach for treating BKPyV in KTRs. This single-center phase I, open-label trial enrolled KTRs with persistent BKPyV-DNAemia and BKPyV nephropathy (BKPyVAN) (NCT05042076) despite being on lower immunosuppression. BK-specific T cells were isolated from leukapheresis products from compatible donors. Patients were treated with VST therapy and followed for 52 weeks. Safety and tolerability were the primary focus of this trial. Three patients completed the trial. No grade III or IV adverse events, acute rejections, or graft versus host disease were reported. All patients tolerated the therapy well, with no significant safety concerns observed during the follow-up period. BK-VST demonstrated promising safety and tolerability profiles in this small cohort of kidney transplant recipients with severe BK infections. These findings suggest that VST therapy may offer a safe adjunctive treatment option for BKPyV infections post-transplantation. Larger studies are needed to confirm these preliminary results and assess long-term efficacy in treating BKPyV infections and preserving graft function in kidney transplant recipients.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 4","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70357","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum to “Reflections Regarding Validation of New HPV Tests With Reduced HPV Genotypes: Report From an IARC Expert Consultation” “关于减少HPV基因型的新HPV检测验证的反思：来自IARC专家咨询的报告”的勘误

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2025-04-17 DOI: 10.1002/jmv.70361

引用次数: 0

Patterns of Co-infection of HPV52 With Other HPV Genotypes and Their Risks of Cervical Precancer and Carcinoma HPV52与其他HPV基因型合并感染的模式及其对宫颈癌前病变和癌的风险

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2025-04-17 DOI: 10.1002/jmv.70312

Yaping Wang, Zhiheng Wang, Wenjie Qu, Fang Chen, Yan Wang, Wenqian Shi, Yingxin Gong, Qi Zhou, Jiayin Mo, Lin Lin, Tianyi Bi, Fangying Chen, Long Sui, Yanyun Li

{"title":"Patterns of Co-infection of HPV52 With Other HPV Genotypes and Their Risks of Cervical Precancer and Carcinoma","authors":"Yaping Wang, Zhiheng Wang, Wenjie Qu, Fang Chen, Yan Wang, Wenqian Shi, Yingxin Gong, Qi Zhou, Jiayin Mo, Lin Lin, Tianyi Bi, Fangying Chen, Long Sui, Yanyun Li","doi":"10.1002/jmv.70312","DOIUrl":"https://doi.org/10.1002/jmv.70312","url":null,"abstract":"<p>Human papillomavirus 52 (HPV52) is the second most frequent HPV type in high-grade squamous intraepithelial lesion (HSIL) cases in China. However, few researchers have explored the co-infection of HPV52 with other HPV genotypes and their correlation with cervical lesions. In this study, 13,809 HPV52-positive patients visiting the Obstetrics and Gynecology Hospital of Fudan University from 2018 to 2023 were included in the first stage to investigate the risk of cervical lesions among different multiple infection patterns. Another 443 HPV52-positive patients were further included for sequence alignment and phylogenetic analysis. In the current study, the most common HPV52 dual-infection patterns were as follows: HPV16 + HPV52, HPV52 + HPV58, HPV52 + HPV53, and HPV52 + HPV81. Compared with HPV52 single infection, the risk of HSIL+ was increased in HPV16 + HPV52 (OR = 3.47, 95% CI: 2.56, 4.69) and HPV52 + HPV58 (OR = 1.99, 95% CI: 1.35, 2.92) groups. The most common triple-infection patterns were HPV16 + HPV52 + HPV53 and HPV52 + HPV53 + HPV81, followed by HPV52 + HPV53 + HPV58. HPV53 was the most common co-infection type with HPV52 in cases of triple or more multiple infections. However, compared with dual infection, the addition of HPV53 did not affect the risk of HSIL+. Two synonymous mutations, G207A (<i>p</i> = 0.029) and C1203T (<i>p</i> = 0.021), showed statistically significant differences in distribution between single and multiple infection groups. Our results demonstrated that HPV52 showed preferences for co-infection with HPV16, 585,381. HPV52 co-infection with HPV16 and HPV58 increased the risk of HSIL+, while co-infection with HPV53 did not increase the risk of HSIL+. Virus variants with certain mutations may be more susceptible to multiple infections.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 4","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70312","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytomegalovirus-RNA Accurately Identifies Clinically Significant Infection Needing Preemptive Therapy in Liver Transplanted Children: A Proof-of-Concept Study 巨细胞病毒- rna准确识别肝移植儿童中需要先发制人治疗的临床显著感染：一项概念验证研究

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2025-04-15 DOI: 10.1002/jmv.70347

Emanuele Nicastro, Eleonora Severi, Ilaria Passera, Michele Totaro, Lorenza Matarazzo, Laura Fornataro, Francesco Morotti, Alessandra Tebaldi, Ezio Bonanomi, Michela Bravi, Angelo Di Giorgio, Samuele Covini, Marta Dolci, Domenico Pinelli, Marco Enrico Giovanni Arosio, Lorenzo D'Antiga

{"title":"Cytomegalovirus-RNA Accurately Identifies Clinically Significant Infection Needing Preemptive Therapy in Liver Transplanted Children: A Proof-of-Concept Study","authors":"Emanuele Nicastro, Eleonora Severi, Ilaria Passera, Michele Totaro, Lorenza Matarazzo, Laura Fornataro, Francesco Morotti, Alessandra Tebaldi, Ezio Bonanomi, Michela Bravi, Angelo Di Giorgio, Samuele Covini, Marta Dolci, Domenico Pinelli, Marco Enrico Giovanni Arosio, Lorenzo D'Antiga","doi":"10.1002/jmv.70347","DOIUrl":"https://doi.org/10.1002/jmv.70347","url":null,"abstract":"<p>Preemptive therapy (PET) is safe and effective in controlling Cytomegalovirus (CMV) infection after pediatric liver transplantation (LT) and allows to observe the kinetics of quantitative CMV-DNA viral load till it reaches the treatment thresholds. While early detection of low-to-moderate CMV-DNA levels may not indicate active viral replication, awaiting the viral load to exceed the treatment threshold may lead to viremic breakthroughs and CMV disease. We assessed the capacity of quantitative CMV-RNA (UL21.5 mRNA) to identify active viral replication and its accuracy in identifying clinically significant CMV infection (csCMVi) needing PET in LT children. One-hundred and forty-four comparative quantitative CMV-RNA and CMV-DNA determinations were obtained from 12 children followed prospectically for 6 months after LT. Of 52 CMV-DNA-positive specimens, 17 (32%) were also CMV-RNA-positive, while CMV-RNA was undetectable in CMV-DNA-negative specimens. All children with csCMVi had early detectable CMV-RNA, peaking simultaneously to CMV-DNA (median CMV-DNA: 65 906 cp/mL; median CMV-RNA: 767 cp/mL); conversely, none of those with persistently low DNAemia proved CMV-RNA-positive. In this first pilot study, CMV-RNA had 100% sensitivity and specificity in identifying children needing PET after pediatric LT. The early detection of CMV-RNA marks significant CMV infection/reactivation, thus allowing to avoid unnecessary antiviral treatment.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 4","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70347","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0