Journal of Medical Virology最新文献

{"title":"Disease Burden of RSV Infection in Adult Patients in Comparison to Influenza Virus Infection","authors":"Georgii Trifonov,&nbsp;Erik Büscher,&nbsp;David Fistera,&nbsp;Clemens Kill,&nbsp;Joachim Risse,&nbsp;Christian Taube,&nbsp;Daniel Todt,&nbsp;Ulf Dittmer,&nbsp;Carina Elsner","doi":"10.1002/jmv.70373","DOIUrl":"https://doi.org/10.1002/jmv.70373","url":null,"abstract":"<p>Respiratory Syncytial Virus (RSV) is well known for its impact on children, but its burden in adults remains underexplored, partly due to limited PCR testing before the COVID-19 pandemic. In this study, the medical burden of RSV infections in adults was retrospectively investigated using 6-year longitudinal data from a university hospital in North Rhine-Westphalia, Germany. Outcomes of 380 PCR-confirmed RSV cases were compared with 1088 influenza A/B cases from 2018 to 2023, stratified by age groups ( &lt; 60 and ≥ 60 years). Among RSV cases, 59.7% required hospitalization, of which 22.9% needed oxygen supply. In the whole group hospitalization rates were comparable between RSV and influenza cases, but oxygen supply was more frequent in influenza infections. However, in patients aged ≥ 60 years, no significant differences were observed in hospitalization, oxygen supply, or fatal outcomes between RSV and influenza, indicating a comparable disease burden for both viruses in this group. These findings highlight the significant clinical impact of RSV in adults, particularly those aged ≥ 60 years, paralleling that of influenza. Given influenza's established pathogenic reputation, this underscores the importance of targeted vaccination strategies against RSV, especially for high-risk age groups.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 5","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70373","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143889109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Evolution Between HIV-1 Groups M and O: HIV-1/MO Recombinant Forms","authors":"Moisan Alice,&nbsp;Tombette Fabienne,&nbsp;Plantier Jean-Christophe","doi":"10.1002/jmv.70358","DOIUrl":"https://doi.org/10.1002/jmv.70358","url":null,"abstract":"<p>HIV exhibits significant genetic diversity, with genetic recombination being a major evolutionary process. The co-circulation of HIV-1/M and HIV-1/O variants has led to the description of 20 HIV-1/M+O dual infections since 1998. Despite the genetic divergence between these variants, HIV-1/M+O dual infections have resulted in the emergence of HIV-1/MO intergroup recombinant forms, with 20 unique HIV-1/MO recombinant forms (URF_MO) currently described, raising the question of a possible benefit of the recombination and the modalities of their emergence. This review summarized the current knowledge of HIV-1/MO recombinant forms, including their virological and genetic characteristics, phylogenetic analysis, genome profiles, and breakpoints number and location. This study also identified the potential impacts of HIV-1/MO recombination on diagnosis, monitoring, and treatment, as well as the replicative capacity of such recombinants. This review highlighted the greater diversity and complexity of HIV-1/MO recombinants than originally thought, offering new research perspectives on their emergence and virological properties.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 5","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70358","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Standard and Rapid Antigen Detection Assays for Human Metapneumovirus","authors":"Fangyuan Zhang,&nbsp;Xiuying Liu,&nbsp;Huarui Duan,&nbsp;Xuehua Yang,&nbsp;Peixiang Gao,&nbsp;Jingya Zhou,&nbsp;Xinhui Zhang,&nbsp;Shengnan Pan,&nbsp;Xuemeng Dong,&nbsp;Yi Liao,&nbsp;Junyu Liu,&nbsp;Zhengde Xie,&nbsp;Xiaojing Chi,&nbsp;Wei Yang","doi":"10.1002/jmv.70374","DOIUrl":"https://doi.org/10.1002/jmv.70374","url":null,"abstract":"<div>\u0000 \u0000 <p>Human metapneumovirus (hMPV), identified as a novel respiratory pathogen in 2001, is responsible for causing acute respiratory illness across various patient demographics. Early detection of hMPV is crucial for administering timely treatment, thereby controlling the virus's propagation. There is a pressing need for the development of a more convenient and expeditious detection strategy for hMPV. The present study focused on the expression and purification of the highly conserved nucleoprotein (N) of hMPV, which served as an antigen in the generation of specific nanobodies and mouse monoclonal antibodies. Subsequently, we evaluated the efficacy of these immunological reagents in detecting the hMPV antigen through the application of double-antibody sandwich enzyme-linked immunosorbent assay and colloidal gold lateral flow chromatography test strips. These detection methods were successfully utilized on the recombinant antigen, cell culture-derived hMPV, and nasopharyngeal swab specimens. The findings offer promising avenues for the development of convenient and rapid detection techniques, which are particularly pertinent during the virus's epidemic seasons.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 5","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outbreak of Enterovirus D68 in Young Children, Brescia, Italy, August to November 2024","authors":"Serena Messali,&nbsp;Anna Bertelli,&nbsp;Laura Dotta,&nbsp;Marta Giovanetti,&nbsp;Leonardo Sclavi,&nbsp;Giulia Venneri,&nbsp;Massimo Ciccozzi,&nbsp;Raffaele Badolato,&nbsp;Arnaldo Caruso,&nbsp;Francesca Caccuri","doi":"10.1002/jmv.70372","DOIUrl":"https://doi.org/10.1002/jmv.70372","url":null,"abstract":"<p>Enterovirus D68 (EV-D68) is responsible for a plethora of clinical manifestations ranging from asymptomatic infections to severe respiratory symptoms and neurological disorders. EV-D68 was first detected in children with pneumonia in 1962 and, from then, only sporadic cases were reported until 2014, when outbreaks were notified across the world. After the withdrawal of preventive measures against SARS-CoV-2, a significant increase in EV-D68 infections has been reported in 2021–2022. A surveillance program to evaluate the incidence of enterovirus/rhinovirus (EV/RV) infections was implemented at the Brescia Civic Hospital, Italy. Fifty-five EV/RV-positive respiratory samples, belonging to pediatric patients, were subjected to NGS. We observed that 61.8% of samples were positive for EV, with EV-D68 as the most prevalent genotype predominantly detected between August and November 2024. Phylogenetic analysis revealed that EV-D68 sequences formed two monophyletic clades corresponding to the A2 and B3 lineages, highlighting their recent introduction in Italy. Interestingly, 40% of pediatric EV-D68 infections were detected with at least one other EV/RV. Our study highlights the crucial role played by genomic surveillance of respiratory infections to monitor the circulation of emerging and re-emerging viruses, as well as their evolution. This will be fundamental to enable prompt intervention strategies.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 5","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70372","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clathrin-Independent Carriers/Glycosylphosphatidylinositol-Anchored-Protein-Enriched Endosomal Compartment Endocytic Pathway Is Critical for Enterovirus A71 Entry Into Human Oral Epidermoid Carcinoma KB Cells","authors":"Zhiwei He,&nbsp;Binghui Xia,&nbsp;Tianqi Zhao,&nbsp;Ping Zhao,&nbsp;Hao Ren,&nbsp;Zhongtian Qi,&nbsp;Yongzhe Zhu","doi":"10.1002/jmv.70369","DOIUrl":"https://doi.org/10.1002/jmv.70369","url":null,"abstract":"<div>\u0000 \u0000 <p>Enterovirus A71 (EV-A71) causes hand, foot, and mouth disease, which can lead to further infections and spreads via the oral cavity; however, the mechanism of how EV-A71 infects the human oral cavity remains unclear. Screening and validation using small-interfering RNAs and chemical inhibitors showed that EV-A71 entry into human oral epidermoid carcinoma KB cells was independent of clathrin-, caveolin-, endophilin-, dynamin-, and macropinocytosis-mediated pathways. However, the clathrin-independent carriers/GPI-anchored-protein-enriched endosomal compartment (CLIC/GEEC) pathway is crucial for EV-A71 entry into KB cells and normal human oral epithelial cells (NHOEC), which requires the entire actin cytoskeleton and membrane cholesterol. Inhibition of GBF1, ARF1, CDC42, PICK1, GRAF1, and IRSp53, the key molecules of the CLIC/GEEC pathway, significantly suppressed EV-A71 entry and infection. Confocal microscopy showed that internalized EV-A71 colocalized with CDC42 and GRAF1. Knockdown of CDC42 or GRAF1 reduced the number of internalized viral particles, which were predominantly localized at the plasma membrane. By using the sucrose density gradient centrifugation, EV-A71 and glycosylphosphatidylinositol-anchored GFP (GPI-GFP) were observed within the same low-density components. Furthermore, endocytosed EV-A71 was colocalized with GRAF1 and GPI-GFP and transported to the late endosomes. Mouse experiments demonstrated that the GBF1 inhibitor, Golgicide A, significantly reduced EV-A71 infection-induced mortality. Immunohistochemical staining and histopathological section analysis revealed that Golgicide A markedly decreased the viral load in brain tissue and oral epithelium, and alleviated the pathological damage induced by the virus in brain tissue. Our findings reveal a novel pathway for EV-A71 entry into KB cells and provide a new target for drug development.</p></div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 5","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic Review and Meta-Analysis of the Association Between Clinical Severity and Co-Infection of Human Adenovirus With Other Respiratory Pathogens in Children","authors":"Dandan Niu,&nbsp;Yanxiao Gao,&nbsp;Yingluan Zhang,&nbsp;Qiuying Lv,&nbsp;Yiwen Jiang,&nbsp;Yuanxi Jia,&nbsp;Zhigao Chen,&nbsp;Honglin Wang,&nbsp;Yanpeng Cheng,&nbsp;Feng Sha,&nbsp;Meng Ren,&nbsp;Yixiong Chen,&nbsp;Xindong Zhang,&nbsp;Zhen Zhang,&nbsp;Jinling Tang,&nbsp;Tiejian Feng","doi":"10.1002/jmv.70370","DOIUrl":"https://doi.org/10.1002/jmv.70370","url":null,"abstract":"<p>The correlation between the co-infection of human respiratory adenovirus (HAdV) and clinical severity has not been firmly established yet. We carried out a systematic review and meta-analysis. We scoured six databases for studies published up to 16 May 2024. A total of 66 cohort studies, which involved 16 251 participants, were incorporated. When compared with patients suffering from HAdV single infection, those with co-infection of viruses (risk ratios [RRs] = 1.40, 95% confidence interval [CI]: 1.05–1.86), bacteria (RR = 1.50, 95% CI: 1.05–2.16), or fungi (RR = 2.86, 95% CI: 2.17–3.76) were more prone to experience severe clinical outcomes. Co-infection with <i>Mycoplasma pneumoniae</i> had a tendency to elevate the risk of common pneumonia (RR = 1.81, 95% CI: 1.66–1.97), and bacterial co-infection was likely to extend the hospital stay (mean differences = 2.23 days, 95% CI: 0.44–4.03). In summary, the co-infection of HAdV with other viral, bacterial, fungal respiratory pathogens or <i>Mycoplasma pneumoniae</i> heightened the risk of severe clinical outcomes in pediatric patients, leading to an increased utilization of medical resources. This implied that the ecological and biological mechanisms underlying the potential interactions between HAdV and other microorganisms merited further investigation.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 5","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70370","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional Cure of Hepatitis B and D Coinfection After ART Nonadherence in a Person With HIV","authors":"Alexander Killer,&nbsp;Smaranda Gliga,&nbsp;Björn-Erik Ole Jensen,&nbsp;Paul Park,&nbsp;Nadine Lübke,&nbsp;Andreas Walker,&nbsp;Jörg Timm,&nbsp;Tom Luedde,&nbsp;Hans H. Bock","doi":"10.1002/jmv.70375","DOIUrl":"https://doi.org/10.1002/jmv.70375","url":null,"abstract":"","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 5","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological Response to Mpox and Direct Virus Detection in Asymptomatic Patient Prior to the First Diagnosed Case: A Retrospective Study of the 2022 Montpellier Epidemic","authors":"Steven Henry,&nbsp;Maeliss Champagne,&nbsp;Ahidjo Ayouba,&nbsp;Martine Peeters,&nbsp;Leo-Pol Rio,&nbsp;Marie Bistoquet,&nbsp;Carmen Alcocer Cordellat,&nbsp;Sylvain Godreuil,&nbsp;Edouard Tuaillon,&nbsp;Vincent Foulongne","doi":"10.1002/jmv.70365","DOIUrl":"https://doi.org/10.1002/jmv.70365","url":null,"abstract":"<p>The Mpox (formerly monkeypox) outbreak in 2022 presented unprecedented challenges, including widespread transmission in non-endemic regions, particularly among men who have sex with men. This study examines Mpox infections in Montpellier, France, focusing on diagnostic testing, serological profiles, and potential asymptomatic cases, based on data from Montpellier University Hospital. We retrospectively analyzed results from 91 patients tested positive for Mpox DNA. Antibody responses were monitored using novel Mpox virus peptide-based serological assays. Our findings highlight that MPXV antibodies can develop early in infection, peaking within 2 weeks to 3 months, though responses varied by antigen type. Additionally, asymptomatic Mpox infections were suggested, with virus detected in blood, urine, anal, oral and genital swabs screened Sexual Transmitted Infection samples. Notably, viral presence was confirmed in Montpellier samples as early as mid-May 2022, before the first known symptomatic case in Hospital, in the same period as the first official case in France. This study underscores the need for expanded screening in high-risk clinics to control Mpox spread and supports the potential utility of serological assays for broad immune profiling. Enhanced diagnostic tools and proactive surveillance in sexual health settings are recommended to improve outbreak response and prevent further transmission.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 5","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70365","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of Subgenomic RT-PCR for Predicting SARS-CoV-2 Infectivity Compared to Genomic RT-PCR and Culture Isolation","authors":"Célia Sentis,&nbsp;Delphine Parraud,&nbsp;Geneviève Billaud,&nbsp;Martine Valette,&nbsp;Maude Bouscambert-Duchamp,&nbsp;Bruno Lina,&nbsp;Florence Morfin,&nbsp;Alexandre Gaymard","doi":"10.1002/jmv.70363","DOIUrl":"https://doi.org/10.1002/jmv.70363","url":null,"abstract":"<p>SARS-CoV-2 clinical samples can be detected as positive for a long period of time using real-time RT-PCR, even when patients are no longer infectious. Viral culture is the gold standard for assessing a patient's infectivity, but it is a time-consuming technique and lacks sensitivity. SARS-CoV-2 subgenomic RNA (sgRNA) detection has been used as a proxy for assessing the infectivity but only a limited number of studies have described its use in vitro and in clinical samples. This study aimed to evaluate the correlation between results from viral culture, genomic RT-PCR (gRT-PCR), and subgenomic RT-PCR (sgRT-PCR) during in vitro infection and in clinical samples. In vitro viral replication kinetics showed that both genomic RNA (gRNA) and subgenomic RNA (sgRNA) levels remained stable up to 21 days in the absence of replication-competent virus. Using clinical samples, sgRNA was detected in 87.5% of culture-positive samples, demonstrating better performances than gRT-PCR (Positive predictive value (PPV) 93.3% and Negative predictive value (NPV) of 87.5%) and an almost perfect agreement with culture results (Cohen <i>κ</i> = 0.81 [95% CI: 0.66–0.95]). These findings suggest that testing for sgRNA and/or using a gRNA Ct cut-off of 21.2 could be used as a proxy to determine the presence of SARS-CoV-2 replication-competent virus.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 5","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70363","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-Acute Sequelae of COVID-19 on Alopecia Areata in Individuals With Mental Illness in South Korea and Japan: A Binational Population-Based Cohort Study","authors":"Jaehyeong Cho,&nbsp;Jaeyu Park,&nbsp;Yejun Son,&nbsp;Soeun Kim,&nbsp;Hyesu Jo,&nbsp;Seoyeon Kyung,&nbsp;Hayeon Lee,&nbsp;Dong Keon Yon","doi":"10.1002/jmv.70364","DOIUrl":"https://doi.org/10.1002/jmv.70364","url":null,"abstract":"<div>\u0000 \u0000 <p>While previous studies have primarily focused on post-acute sequelae of COVID-19, the long-term effects of SARS-CoV-2 infection on alopecia areata (AA) among individuals with mental illness remain underexplored. Thus, this study aimed to address this gap by examining the association between post-acute sequelae of COVID-19 and AA, with a specific focus on individuals with mental illness. This study utilized bi-national, large-scale, and population-based cohorts of individuals with pre-existing mental illness: a Korean nationwide cohort (K-COV-N cohort; main cohort; total <i>n</i> = 3 248 448) and a Japanese claims-based cohort (JMDC cohort; replication cohort; total <i>n</i> = 696 332). The outcome focused on the new onset of AA following 30 days after SARS-CoV-2 infection, defined using ICD-10 codes L63. Using a propensity score-based overlap weighted algorithm, the adjusted hazard ratio (aHR) for AA following COVID-19 was calculated for individuals with mental illness. In the main cohort, the risk of AA as post-acute sequelae of COVID-19 was identified among individuals with mental illness (aHR, 1.32 [95% CI, 1.23–1.43]). The risk was significant for mild mental illness (aHR, 1.33 [95% CI, 1.24–1.44]) and within 6 months postinfection (aHR, 1.48 [95% CI, 1.35–1.63]). Similar findings were observed in the replication cohort. In conclusion, among individuals with mental illness, the risk of post-acute sequelae of COVID-19 on AA was elevated—particularly in those with mild mental illness—though this risk decreased over time. Our findings highlight the importance of early screening, integrated care, and equitable healthcare access for managing post-acute sequelae of COVID-19.</p></div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 5","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143871682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}