Journal of Medical Virology最新文献

Intravenous immunoglobulin‑based adjuvant therapy for severe fever with thrombocytopenia syndrome: A single‑center retrospective cohort study 严重发热伴血小板减少综合征的静脉注射免疫球蛋白辅助治疗：单中心回顾性队列研究。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-11-04 DOI: 10.1002/jmv.70017

Yu Zhai, Haopeng Li, Peng Xia, Yunfei Jiang, Hanwen Tong, Dongming Zhou, Chenxiao Jiang, Yun Liu, Jun Wang

{"title":"Intravenous immunoglobulin‑based adjuvant therapy for severe fever with thrombocytopenia syndrome: A single‑center retrospective cohort study","authors":"Yu Zhai, Haopeng Li, Peng Xia, Yunfei Jiang, Hanwen Tong, Dongming Zhou, Chenxiao Jiang, Yun Liu, Jun Wang","doi":"10.1002/jmv.70017","DOIUrl":"10.1002/jmv.70017","url":null,"abstract":"Intravenous immunoglobulin (IVIG) is frequently administered to patients with severe fever with thrombocytopenia syndrome (SFTS), particularly those with severe manifestations, although its efficacy remains controversial. The study retrospectively analyzed the effects of IVIG administration on SFTS patients in both mild and severe groups. The primary outcome measure was 28-day mortality. Inverse probability of treatment weighting (IPTW) with propensity score was used to account for baseline confounders. A total of SFTS patients with complete data enrolled from January 1, 2015, to August 1, 2023. Death at 28 days occurred for 68 (17.5%) patients. By unadjusted analysis, no difference was observed for 28-day mortality between the IVIG and non-IVIG groups in both the mild and severe groups. Similar results were found by propensity score matching and by IPTW analysis. Although IVIG is frequently used as adjuvant therapy for severe SFTS patients, no significant association was observed between IVIG treatment and reduced mortality in this patient population.","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BK Polyomavirus Infection of Bladder Microvascular Endothelial Cells Leads to the Activation of the cGAS-STING Pathway BK 多瘤病毒感染膀胱微血管内皮细胞导致 cGAS-STING 通路激活

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-11-02 DOI: 10.1002/jmv.70038

Kateřina Bruštíková, Boris Ryabchenko, David Liebl, Lenka Horníková, Jitka Forstová, Sandra Huérfano

{"title":"BK Polyomavirus Infection of Bladder Microvascular Endothelial Cells Leads to the Activation of the cGAS-STING Pathway","authors":"Kateřina Bruštíková, Boris Ryabchenko, David Liebl, Lenka Horníková, Jitka Forstová, Sandra Huérfano","doi":"10.1002/jmv.70038","DOIUrl":"10.1002/jmv.70038","url":null,"abstract":"BK polyomavirus (BKPyV) infection in humans is usually asymptomatic but ultimately results in viral persistence. In immunocompromised hosts, virus reactivation can lead to nephropathy or hemorrhagic cystitis. The urinary tract serves as a silent reservoir for the virus. Recently, it has been demonstrated that human bladder microvascular endothelial cells (HBMVECs) serve as viral reservoirs, given their unique response to infection, which involves interferon (IFN) production. The aim of the present study was to better understand the life cycle of BKPyV in HBMVECs, uncover the molecular pathway leading to IFN production, and to identify the connection between the viral life cycle and the activation of the IFN response. Here, in the early stage of infection, BKPyV virions were found in internalized monopinocytic vesicles, while later they were detected in late endosomes, lysosomes, tubuloreticular structures, and vacuole-like vesicles. The production of viral progeny in these cells started at 36 h postinfection. Increased cell membrane permeability and peaks of virion release coincided with the leakage of viral and cellular DNA into the cytosol at approximately 60 h postinfection. Leaked DNA colocalized with and activated cGAS, leading to the activation of STING and the consequent transcription of IFNB and IFN-related genes; in contrast, the IFN response was attenuated by exposure to the cGAS inhibitor, G140. These findings highlight the importance of the cGAS-STING pathway in the innate immune response of HBMVECs to BKPyV.","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fourth-Generation HIV Rapid Tests: Enhanced Sensitivity and Reduced Diagnostic Window for HIV-1 Primary Infection Screening 第四代 HIV 快速检测试剂盒：第四代 HIV 快速检测试剂盒：提高 HIV-1 初诊感染筛查的灵敏度并缩短诊断窗口期。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-11-02 DOI: 10.1002/jmv.70044

Vincent Guiraud, Angèle Naizet, Habiba Khan, Ghizlane Benhafoun, Pierre Hernandez, Luigi Piccin, Agnès Pichon, Ay Ling Leng, Léna Yousfi, Agnès Gautheret-Dejean

{"title":"Fourth-Generation HIV Rapid Tests: Enhanced Sensitivity and Reduced Diagnostic Window for HIV-1 Primary Infection Screening","authors":"Vincent Guiraud, Angèle Naizet, Habiba Khan, Ghizlane Benhafoun, Pierre Hernandez, Luigi Piccin, Agnès Pichon, Ay Ling Leng, Léna Yousfi, Agnès Gautheret-Dejean","doi":"10.1002/jmv.70044","DOIUrl":"10.1002/jmv.70044","url":null,"abstract":"As most HIV rapid tests (HRT) detect only HIV-1/2 antibodies, their performance during primary HIV infection is poor. Determine HIV Early detect (Abbott) (Determine) is the only HRT with an HIV-1 p24-antigen detection, but the impact of this addition in shortening the diagnostic window remains unclear. A total of 183 HIV-1 primary infection samples were tested using the HRTs Determine and ONE STEP anti-HIV (1&2) Test (InTec Products) (One-Step). The pre-seroconversion subgroup was defined as p24-antigen positivity without Western blot nor Liaison XL (fouth generation enzyme immunoassay with distinct signal for p24-antigen and HIV-1 antibody) HIV-1 antibodies. Global sensitivity (95% CI) was 95% (91–97) for Determine versus 80% (74%–85%) for One-Step (difference p = 1.38e−06). Pre-seroconversion subgroup sensitivity was lower, at 71.9 (54.6%-84.4%) for Determine and 9.7% (3.3%–24.9%) for One-Step. Among the 45 samples with an HIV-1 infection date, no HRT was reactive up to 2 weeks. Between 2 and 3 weeks, Determine sensitivity was 78% (45%–95%) versus 56% (27%–81%) for One-Step. From 3 weeks to 1 month Determine sensitivity was 90% (62%–98%) and One-Step 45% (21%–72%). The last negative sample occurred at 3 weeks for Determine versus 70–90 days for One-Step. HRT with p24-antigen detection significantly shortens the diagnostic window from approximatively 3 months to 1 month. HRTs should be used with caution in the first month after HIV infection.","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Torque Teno Virus Control by the Classical Pathway of Complement Activation-A Retrospective Analysis From a First-in-Human Trial Utilizing Sutimlimab. 通过补体激活的经典途径控制Torque Teno病毒--使用Sutimlimab的首次人体试验的回顾性分析。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-11-01 DOI: 10.1002/jmv.70039

Sebastian Kapps, Jakob Mühlbacher, Dorian Kulifaj, Sophie Courjal, Farsad Eskandary, Martin Schiemann, Bernd Jilma, Georg A Böhmig, Gregor Bond, Markus Wahrmann

{"title":"Torque Teno Virus Control by the Classical Pathway of Complement Activation-A Retrospective Analysis From a First-in-Human Trial Utilizing Sutimlimab.","authors":"Sebastian Kapps, Jakob Mühlbacher, Dorian Kulifaj, Sophie Courjal, Farsad Eskandary, Martin Schiemann, Bernd Jilma, Georg A Böhmig, Gregor Bond, Markus Wahrmann","doi":"10.1002/jmv.70039","DOIUrl":"https://doi.org/10.1002/jmv.70039","url":null,"abstract":"Torque Teno virus (TTV) load is linked with the functionality of its host's immune system and has been proposed as a potential monitoring tool for immune-modulating therapy. However, the immunological mechanisms of TTV control are incompletely understood. To assess the effect of the classical complement pathway on TTV, 64 healthy volunteers and 10 kidney transplant recipients treated with the anti-C1s antibody sutimlimab were analyzed for serum TTV copy numbers (c/mL) by qPCR. Overall, a correlation was observed between the decrease in complement activity caused by sutimlimab and the TTV load increase (ρ = -0.367, p < 0.001). Subgroup analysis indicated a trend toward TTV load increase in healthy volunteers following the highest sutimlimab dose compared to baseline (100 mg/kg body weight; median 3.5 log10 c/mL, interquartile range [IQR] 2.8-4.4 vs. 2.9 log10 c/mL, 0.8-3.5; p = 0.063). Administering multiple lower doses (30 mg/kg) also showed a trend toward TTV load increase in healthy volunteers (1.8 log10 c/mL, 0-2.3 vs. 1.9, 1.3-2.8; p = 0.054) and a significant increase in transplant recipients (3.5 log10 c/mL, 3.0-6.1 vs. 4.1, 3.5-6.4; p = 0.004). This report suggests a role for the classical complement pathway in controlling TTV load.","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stimulator of interferon genes (STING) inhibits coronavirus infection by disrupting viral replication organelles 干扰素基因刺激器（STING）通过破坏病毒复制细胞器来抑制冠状病毒感染。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-10-29 DOI: 10.1002/jmv.70020

Kun Song, Abdul Hasan, Wenzhuo Hao, Yakun Wu, Yiwen Sun, Wenjun Li, Lingyan Wang, Shitao Li

{"title":"Stimulator of interferon genes (STING) inhibits coronavirus infection by disrupting viral replication organelles","authors":"Kun Song, Abdul Hasan, Wenzhuo Hao, Yakun Wu, Yiwen Sun, Wenjun Li, Lingyan Wang, Shitao Li","doi":"10.1002/jmv.70020","DOIUrl":"10.1002/jmv.70020","url":null,"abstract":"Stimulator of interferon genes (STING) is an endoplasmic reticulum (ER) protein that plays a crucial role in cytosolic DNA-mediated innate immunity. Both STING agonists and antagonists have demonstrated their ability to enhance mouse survival against coronavirus, however, the physiological role of endogenous STING in coronavirus infection remains unclear. Our research unveils that STING inhibits coronavirus replication by impeding the formation of the ER-derived double-membrane vesicles (DMVs), the organelles in which coronavirus replicates. We found that STING was still capable of inhibiting coronavirus OC43 infection in cells, regardless of the knockout of cGAS or MAVS, or blocking type I interferon receptor. Moreover, STING disrupted the interaction between two crucial proteins, NSP4 and NSP6, involved in DMV formation, leading to the disruption of DMV formation. Taken together, our study sheds light on a novel antiviral role of STING in coronavirus infection, elucidating how it disrupts the formation of viral replication organelles, thereby impeding the replication process.","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of 2022 Outbreak MPXV and Previous Clade II MPXV 2022 年爆发的 MPXV 与之前的 Clade II MPXV 的对比分析。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-10-28 DOI: 10.1002/jmv.70023

Joseph Patrick McGrail, Alberto Paniz Mondolfi, Juan David Ramírez, Santiago Vidal, Adolfo García-Sastre, Gustavo Palacios, Mari Paz Sanchez-Seco, Susana Guerra

{"title":"Comparative Analysis of 2022 Outbreak MPXV and Previous Clade II MPXV","authors":"Joseph Patrick McGrail, Alberto Paniz Mondolfi, Juan David Ramírez, Santiago Vidal, Adolfo García-Sastre, Gustavo Palacios, Mari Paz Sanchez-Seco, Susana Guerra","doi":"10.1002/jmv.70023","DOIUrl":"10.1002/jmv.70023","url":null,"abstract":"The 2022–2024 outbreak of MPOX is an important worldwide public health issue that has triggered significant concerns in the scientific community. MPOX is caused by monkeypox virus (MPXV) belonging to the Poxviridae family. The study of MPXV presents a multifaceted challenge due to the diverse viral formThis study was supported by ISIDORe consortium and Agencia Estatal de Investigación.s produced by this pathogen. Notably the intracellular mature viruses (MVs) primarily contribute to localized lesions and host-to-host transmission, while the extracellular enveloped viruses (EVs) are associated with systemic infection. Clinically, MPOX manifests as a vesiculopustular rash that initially emerges on the face and trunk, subsequently spreading throughout the body, with heightened severity observed in immunocompromised individuals. Results obtained in this manuscript indicate that the 2022 outbreak MPXV has a significantly slower viral cycle compared with previous Clade II strains, with WRAIR 7-61 being more intermediate and USA 2003 producing highest viral titers. Additionally, proteomic and phospho-proteomic analysis displays differences in protein expression between these three strains. These findings highlight key differences between the current Lineage B.1 MPXV and previous strains. Further studies will be undertaken to demonstrate if these differences are important for the apparent increased human-to-human transmission mechanisms observed in the Clade IIb MPXV outbreak.","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous Versus Homologous COVID-19 Boosters: Immune Response Outcomes in Renal Transplant Recipients 异源与同源 COVID-19 增强剂：肾移植受者的免疫反应结果。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-10-28 DOI: 10.1002/jmv.70030

Yesim Yildiz, Emre Yasar, Erensu Ozturk, Mine Sebnem Karakan, Ozant Helvaci, Hasan Selcuk Ozger, Z. Cemre Araz, Pinar Aysert Yildiz, Asiye Ugras Dikmen, Kayhan Caglar, Murat Dizbay, Ulver Derici, Galip Guz

{"title":"Heterologous Versus Homologous COVID-19 Boosters: Immune Response Outcomes in Renal Transplant Recipients","authors":"Yesim Yildiz, Emre Yasar, Erensu Ozturk, Mine Sebnem Karakan, Ozant Helvaci, Hasan Selcuk Ozger, Z. Cemre Araz, Pinar Aysert Yildiz, Asiye Ugras Dikmen, Kayhan Caglar, Murat Dizbay, Ulver Derici, Galip Guz","doi":"10.1002/jmv.70030","DOIUrl":"10.1002/jmv.70030","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 We aimed to investigate the immune responses to homologous and heterologous COVID-19 booster vaccinations in renal transplant recipients (RTRs) and to identify factors affecting these responses. In this prospective multicenter observational study, we measured the antibody kinetics of 90 RTRs using the chemiluminescent microparticle immunoassay method. The mean age of participants was 45.2 ± 11.4 years, with 35.6% being female. On the 42nd day after the first vaccine dose, the median antibody level was 16.7 (IQR 2.5−249.5) AU/mL, and the seropositivity rate was 60% (n = 36). Mycophenolic acid (MFA) (OR: 0.087, 95% CI: 0.024−0.311) and ACE inhibitor use (OR: 0.203, 95% CI: 0.052−0.794) were identified as independent factors affecting seropositivity. Patients who received the Pfizer/BioNTech booster had significantly higher antibody levels compared to those who received the CoronaVac/Sinovac booster (p = 0.021). Additionally, a significantly higher rate of COVID-19 positivity was observed among patients who received the CoronaVac/Sinovac booster (p = 0.031). Heterologous COVID-19 booster vaccination is significantly more effective than homologous inactivated booster vaccination in enhancing immune responses and preventing new infections in RTRs. MFA and ACE inhibitor usage were independent factors affecting seropositivity. Additional COVID-19 vaccine doses are needed in this patient group.\u0000 </section>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obesity's Unexpected Influence: Reduced Alphavirus Transmission and Altered Immune Activation in the Vector 肥胖的意外影响：减少阿尔法病毒传播和改变病媒的免疫激活。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-10-28 DOI: 10.1002/jmv.70032

Pallavi Rai, Emily M. Webb, Sally L. Paulson, Lin Kang, James Weger-Lucarelli

{"title":"Obesity's Unexpected Influence: Reduced Alphavirus Transmission and Altered Immune Activation in the Vector","authors":"Pallavi Rai, Emily M. Webb, Sally L. Paulson, Lin Kang, James Weger-Lucarelli","doi":"10.1002/jmv.70032","DOIUrl":"10.1002/jmv.70032","url":null,"abstract":"Chikungunya virus (CHIKV) and Mayaro virus (MAYV) are emerging/re-emerging alphaviruses transmitted by Aedes spp. mosquitoes and responsible for recent disease outbreaks in the Americas. The capacity of these viruses to cause epidemics is frequently associated with increased mosquito transmission, which in turn is governed by virus−host−vector interactions. Although many studies have explored virus−vector interactions, significant gaps remain in understanding how vertebrate host factors influence alphavirus transmission by mosquitoes. We previously showed that obesity, a ubiquitous vertebrate host biological factor, reduces alphavirus transmission potential in mosquitoes. We hypothesized that alphavirus-infected obese bloodmeals altered immune genes and/or pathways in mosquitoes, thereby inhibiting virus transmission. To test this, we conducted RNA sequencing (RNA-seq) and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) on midgut RNA from mosquitoes fed on alphavirus-infected lean and obese mice. This approach aimed to identify potential antiviral or proviral genes and pathways altered in mosquitoes after consuming infected obese bloodmeals. We found upregulation of the Toll pathway and downregulation of several metabolic and other genes in mosquitoes fed on alphavirus-infected obese bloodmeals. Through gene knockdown studies, we demonstrated the antiviral role of Toll pathway and proviral roles of AAEL009965 and fatty acid synthase (FASN) in the transmission of alphaviruses by mosquitoes. Therefore, this study utilized obesity to identify factors influencing alphavirus transmission by mosquitoes and this research approach may pave the way for designing broadly effective antiviral measures to combat mosquito-borne viruses, such as releasing transgenic mosquitoes deficient in the identified genes.","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and antigenic characterization of influenza A(H3N2) virus after 13 consecutive years of influenza surveillance in Senegal, 2010–2022 2010-2022 年塞内加尔连续 13 年流感监测后甲型 H3N2 流感病毒的基因和抗原特征。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-10-23 DOI: 10.1002/jmv.70010

Mamadou Malado Jallow, Mamadou Aliou Barry, Ndiendé Koba Ndiaye, Cheikh Talibouya Touré, Cheikh Talla, Davy Kiori, Samba Niang Sagne, Sara Sy, Deborah Goudiaby, Mbayame Ndiaye Niang, Moussa Moise Diagne, Gamou Fall, Cheikh Loucoubar, Ndongo Dia

{"title":"Genetic and antigenic characterization of influenza A(H3N2) virus after 13 consecutive years of influenza surveillance in Senegal, 2010–2022","authors":"Mamadou Malado Jallow, Mamadou Aliou Barry, Ndiendé Koba Ndiaye, Cheikh Talibouya Touré, Cheikh Talla, Davy Kiori, Samba Niang Sagne, Sara Sy, Deborah Goudiaby, Mbayame Ndiaye Niang, Moussa Moise Diagne, Gamou Fall, Cheikh Loucoubar, Ndongo Dia","doi":"10.1002/jmv.70010","DOIUrl":"10.1002/jmv.70010","url":null,"abstract":"Despite decades of influenza surveillance in many African countries, little is known about the evolutionary dynamics of seasonal influenza viruses. This study aimed to characterize the epidemiological, genetic and antigenic profiles of A/H3N2 viruses in Senegal from 2010 to 2022. A/H3N2 infection was confirmed using reverse transcription-polymerase chain reaction. Subsequently, a representative of A/H3N2 isolates was selected for genome sequencing. Predicted vaccine efficacy was measured using the Pepitope model. During the study period, 22638 samples were tested and influenza was detected in 31.8%, among which type A was confirmed in 78.1%. Of the Influenza A cases, the H3N2 subtype was detected in 29.8%, peaking at expected times during the rainy season. Genome sequencing of 123A/H3N2 isolates yielded 24 complete and 99 partial genomic sequences. Phylogenetic analysis revealed the circulation of multiple clades of A/H3N2 in Senegal, including 2a.3, 3C.2 and 3C.3a. A/H3N2 isolates were mainly susceptible to the influenza antiviral drugs oseltamivir and zanamivir, but the primary adamantine-resistance marker, S31N was encountered in all isolates. At least nine potential N-linked glycosylation sites were predicted among A/H3N2 strains, six of which (at positions 24, 38, 79, 181, 262 and 301) remains conserved among all isolates. Antigenic distances between circulating strains and vaccine viruses indicated varying vaccine efficacies, from suboptimal to moderate protection. The findings emphasize the need to enhance local genomic and antigenic surveillance and further research on influenza epidemiology and genetic evolution in sub-Saharan Africa.","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continuous Global Improvement of Human Papillomavirus (HPV) Genotyping Services: The 2022 and 2023 HPV LabNet International Proficiency Studies 全球人类乳头瘤病毒 (HPV) 基因分型服务的持续改进：2022 年和 2023 年 HPV LabNet 国际能力研究。

IF 6.8 3区医学

Journal of Medical Virology Pub Date : 2024-10-22 DOI: 10.1002/jmv.70022

Laila Sara Arroyo Mühr, Carina Eklund, Camilla Lagheden, Emel Yilmaz, Ola Forslund, Marina Lilja, Joakim Dillner

{"title":"Continuous Global Improvement of Human Papillomavirus (HPV) Genotyping Services: The 2022 and 2023 HPV LabNet International Proficiency Studies","authors":"Laila Sara Arroyo Mühr, Carina Eklund, Camilla Lagheden, Emel Yilmaz, Ola Forslund, Marina Lilja, Joakim Dillner","doi":"10.1002/jmv.70022","DOIUrl":"10.1002/jmv.70022","url":null,"abstract":"The International Human Papillomavirus (HPV) Reference Center launches annual global HPV genotyping proficiency panels to enhance the precision and international standardization of HPV genotyping services. This study aims to assess the proficiency levels achieved in the global HPV genotyping proficiency panels conducted in 2022 and 2023, and to evaluate trends in performance over time. The proficiency panels comprised 44 blinded samples each, including 40 samples containing various purified plasmids corresponding to HPV types combined with human DNA, plus four control samples (one negative control and three extraction controls). Proficiency required a sensitivity of 50 International Units (IU)/5 µL for HPV 16 and HPV 18 500 IU/5 µL for HPVs 6, 11, 31, 33, 45, 52, and 58 and 500 genome equivalents (GE)/5 µL for other HPV types in both single and multiple infections, while avoiding false positivity. In 2022, 78 laboratories submitted a total of 154 data sets, and in 2023, 81 laboratories contributed 141 data sets. Most data sets (87%, 258/295) utilized commercially available HPV assays. Proficiency was common, with 77% of data sets meeting the proficiency criteria in 2022 and 79% in 2023. False positive results significantly decreased from 22% in 2022 to 13% in 2023. The high proficiency and increasing specificity in HPV genotyping services indicates progress toward more reliable HPV testing. High accuracy is crucial for supporting global efforts in HPV and cervical cancer elimination.","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":null,"pages":null},"PeriodicalIF":6.8,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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