Journal of Medical Virology最新文献

{"title":"Detection of Measles Virus Genotype D8 in Wastewater of the Brussels Capital Region, Belgium","authors":"Annabel Rector,&nbsp;Mandy Bloemen,&nbsp;Bart Hoorelbeke,&nbsp;Marc Van Ranst,&nbsp;Elke Wollants","doi":"10.1002/jmv.70251","DOIUrl":"https://doi.org/10.1002/jmv.70251","url":null,"abstract":"<p>We analyzed wastewater from Belgian treatment plants to look for measles virus. Genotype D8 was identified in Brussels North samples, matching sequences from 15 regional measles cases. Finding measles virus in wastewater can suggest undetected virus transmission. Wastewater surveillance is a valuable tool for identifying viral circulation and supporting public health interventions against outbreaks.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70251","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Polo-Like Kinase 1 Dampens the Replication of Vaccinia Virus in Mammalian Cells","authors":"Junda Zhu,&nbsp;Xuejiao Jia,&nbsp;Shuning Ren,&nbsp;Zihui Zhang,&nbsp;Hua Li,&nbsp;Jing Wang,&nbsp;Baifen Song,&nbsp;Wenxue Wu,&nbsp;Chen Peng","doi":"10.1002/jmv.70240","DOIUrl":"https://doi.org/10.1002/jmv.70240","url":null,"abstract":"<div>\u0000 \u0000 <p>Since the eradication of smallpox, zoonotic poxviruses, such as the mpox virus (MPXV), continue to pose a threat to public health. Identifying drugs that reduce poxvirus infection and replication, as well as understanding their molecular mechanisms, is essential for epidemic control. Polo-like kinase 1 (PLK1) has been shown to facilitate vaccinia virus (VACV) infection and replication. This study confirms the effects of the PLK1 inhibitors HMN-214 and ON-01910 on VACV replication in A549 cells. Both viral titers and DNA loads were significantly reduced in treated cells after infection. Additionally, ON-01910 demonstrated broad-spectrum antiviral activity against the lumpy skin disease virus (LSDV) and the infectious bovine rhinotracheitis virus (IBRV) in vitro. PLK1 knockdown in A549 cells also led to a reduction in VACV protein expression, viral titers, and DNA levels. Further analysis showed that VACV infection leads to the accumulation of PLK1 near viral factories. However, despite its strong in vitro effects, ON-01910 did not significantly reduce VACV replication in mice. These findings highlight the critical role of PLK1 in VACV replication and its potential as a target for antiviral therapy against orthopoxviruses.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiological Investigations for Chikungunya Virus in Children With Acute Encephalitis Syndrome in a Non-Outbreak Setting in Southern India","authors":"Tina Damodar,&nbsp;Chitra Pattabiraman,&nbsp;Bhagteshwar Singh,&nbsp;Maria Jose,&nbsp;Namratha Prabhu,&nbsp;Akhila L,&nbsp;Pramada Prasad,&nbsp;Uddhava V. Kinhal,&nbsp;A. V. Lalitha,&nbsp;Fulton Sebastian Dsouza,&nbsp;Sushma Veeranna Sajjan,&nbsp;Vykuntaraju K. Gowda,&nbsp;Vasanthapuram Ravi,&nbsp;Ruwanthi Kolamunnage-Dona,&nbsp;Benedict D. Michael,&nbsp;Tom Solomon,&nbsp;Ravi Yadav,&nbsp;Lance Turtle","doi":"10.1002/jmv.70233","DOIUrl":"https://doi.org/10.1002/jmv.70233","url":null,"abstract":"<p>Chikungunya virus (CHIKV) is an emerging cause of acute encephalitis syndrome (AES) in India, with limited data on its role in childhood AES in southern India. We systematically evaluated children with AES in southern India during a non-epidemic period for CHIKV. Serum and cerebrospinal fluid (CSF) samples were tested for CHIKV using IgM ELISA and real-time reverse transcriptase PCR. Amplicon sequencing was performed on PCR-positive samples. Clinical and laboratory features were compared between children with and without CSF CHIKV positivity (PCR/IgM antibodies). Of 376 children with AES, 20 (5.3%) had positive CHIKV tests. Co-infections were common, particularly with scrub typhus. Children presented with diverse symptoms affecting various organ systems. Neurological manifestations included meningism, seizures, cerebellar signs, behavioral abnormalities, cranial nerve involvement, involuntary movements, and hemiparesis/hemiplegia. Children with CSF CHIKV positivity showed more focal neurological deficits and transaminitis, and less musculoskeletal symptoms. Sequencing confirmation of CHIKV was made in all patients with positive CHIKV PCR, revealing a close relationship with 2016 Kenyan and Indian strains, albeit in a different clade within the East/Central/South African genotype. Along with important mutations known to impact CHIKV infectivity, four novel amino acid substitutions were detected in envelope protein coding regions. Our findings underscore the importance of routine and comprehensive CHIKV testing for children with AES, irrespective of season/outbreak. The high rate of co-infections warrants further research. Continued genomic surveillance is essential to monitor emerging mutations with epidemic potential, increased severity and the risk of neurological disease.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70233","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RT-RPA Assisted CRISPR/Cas12a Based One-Pot Rapid and Visual Detection of the Pan-Dengue Virus","authors":"Pooja Bhardwaj,&nbsp;Preeti Dhangur,&nbsp;Alagarasu Kalichamy,&nbsp;Rajeev Singh","doi":"10.1002/jmv.70219","DOIUrl":"https://doi.org/10.1002/jmv.70219","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>Globally ≤ 4 billion of the population are at potential risk of contracting dengue virus (DENV) infection. Seasonal outbreaks of dengue are frequently reported causing a high healthcare burden. Undiagnosed DENV can lead to severe morbidity and mortality. Early diagnosis of DENV relies on molecular methods, which are impractical in resource-constrained settings (RCSs). Dengue can be caused by any of the four distinct DENV serotypes. Therefore, a simple method for rapid diagnosis of Pan-DENV serotypes is of utmost importance at RCSs. A fluorescence detection platform for Pan-DENV using RT-RPA and CRISPR/Cas12a was developed targeting nonstructural 1 (<i>NS1</i>) gene for DENV-1, 2, and 3, and envelope (<i>E</i>) gene for DENV-2. Further, crRNA specific to DENV serotypes were designed to facilitate CRISPR/Cas12a detection. Analytical sensitivity was determined using synthetic RNA and DENV serotypes genome. Clinical validation of the assay was performed using RNA extracted from AES/AFI clinical samples. The developed CRISPR/Cas12a-based detection platform can detect all four serotypes of DENV viz 1−4 in a single pot using fluorescence detection. This assay showed the limit of detection ≥ 781 zg reaction⁻¹, ≥ 1.81 ag reaction⁻¹, ≥ 62.5 fg reaction⁻¹, and ≥ 2.5 pg reaction⁻¹ for synthetic DENV-1, DENV-2, DENV-3, and DENV-4 template, respectively. Our assay demonstrated the analytic sensitivity of ≥ 10 ng reaction⁻¹ for DENV-1 and DENV-4, and ≥ 0.5 ng reaction⁻¹ for DENV-3 and DENV-4 genomes. This assay showed no cross-reactivity with other related etiologies tested causing AFI/AES. With 76 clinical samples (DENV PCR positive = 16, DENV PCR negative = 60), the assay demonstrated 93.7% sensitivity and 100% specificity with an overall accuracy of 98.7% for detection of the Pan-DENV serotypes. Our assay displayed comparable results to that of RT-PCR. The ease of interpretation and rapid detection of the Pan-DENV, represents the potential of the developed assay as an ideal point-of-care test. This assay upon field-deployment could help in reducing healthcare burden, provide differential diagnosis and support initiating early and prompt treatment to patients at RCS.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor Necrosis Factor-Alpha Inhibits the Replication of Patient-Derived Archetype BK Polyomavirus While Activating Rearranged Strains","authors":"Lise Lauterbach-Rivière,&nbsp;Lucia Thuringer,&nbsp;Pascal Feld,&nbsp;Lina Kathrin Toews,&nbsp;Jessica Schüssler,&nbsp;Jonas Klinz,&nbsp;Lars Gläser,&nbsp;Stefan Lohse,&nbsp;Anna Sternjakob,&nbsp;Gilles Gasparoni,&nbsp;Kathrin Kattler-Lackes,&nbsp;Jörn Walter,&nbsp;Marcel A. Lauterbach,&nbsp;Sven Rahmann,&nbsp;Lars Möller,&nbsp;Michael Laue,&nbsp;Martin Janssen,&nbsp;Michael Stöckle,&nbsp;David Schmit,&nbsp;Danilo Fliser,&nbsp;Sigrun Smola","doi":"10.1002/jmv.70210","DOIUrl":"https://doi.org/10.1002/jmv.70210","url":null,"abstract":"<p>To date, no drugs are approved for BK polyomavirus (BKPyV) reactivation, a major cause of nephropathy after kidney transplantation. Recently, tumor necrosis factor-α (TNF-α) blockade has been proposed as a promising therapy, however, the effect of TNF-α on the clinically most common archetype (ww) BKPyV remained unclear. Assays in primary renal proximal tubule epithelial cells (RPTEC) allowed efficient replication only of BKPyV strains with rearranged (rr) non-coding control regions (NCCR), which may develop at later disease stages, but not of ww-BKPyV. Here, we optimized culture conditions allowing robust replication of patient-derived ww-BKPyV, while efficiently preserving their ww-NCCR. TNF-α promoted rr-BKPyV replication, while the T_H1 cytokine IFN-γ suppressed it, also in the presence of TNF-α. Surprisingly, TNF-α alone was sufficient to suppress all ww-BKPyV strains tested. Comprehensive analysis using siRNAs, and chimeric or mutated BKPyV-strains revealed that the response to TNF-α depends on the NCCR type, and that the NF-κB p65 pathway but not the conserved NF-κB binding site is essential for the TNF-α-induced enhancement of rr-BKPyV replication. Our data suggest that in immunosuppressed patients with archetype-dominated infections, TNF-α blockade could interfere with natural TNF-α-mediated anti-BKPyviral control, and this could be detrimental when IFN-γ-driven T_H1 responses are impaired. Ongoing inflammation, however, could lead to the selection of rearrangements responding to NCCR-activating pathways downstream of NF-κB p65 signaling, that may overcome the initial TNF-α-mediated suppression. Our findings also highlight the importance of using clinically relevant BKPyV isolates for drug testing and discovery, for which this new assay paves the way.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70210","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Five-Year (2017–2022) Evolutionary Dynamics of Human Coronavirus HKU1 in Southern France With Emergence of Viruses Harboring Spike H512R Substitution","authors":"Houmadi Hikmat,&nbsp;Lorlane Le Targa,&nbsp;Céline Boschi,&nbsp;Justine Py,&nbsp;Aurélie Morand,&nbsp;Jean-Christophe Lagier,&nbsp;Sarah Aherfi,&nbsp;Jacques Fantini,&nbsp;Bernard La Scola,&nbsp;Philippe Colson","doi":"10.1002/jmv.70217","DOIUrl":"https://doi.org/10.1002/jmv.70217","url":null,"abstract":"<p>HCoV-HKU1 diversity and evolution were scarcely studied. We performed next-generation sequencing (NGS) and analysis of HCoV-HKU1 genomes over 5 years. NGS used Illumina technology on NovaSeq 6000 following whole genome PCR amplification by an in-house set of primers designed using Gemi and PrimalScheme. Genome assembly and analyses used CLC Genomics, Mafft, BioEdit, Nextstrain, Nextclade, MEGA, and iTol bioinformatic tools. Spike molecular modeling and dynamics simulations used Molegro Molecular Viewer and Hyperchem programs. Twenty-eight PCR systems allowed obtaining 158 HCoV-HKU1 genomes including 69 and 89 of genotypes A and B, respectively. Both genotypes co-circulated during the study period but one predominated each year. A total of 1683 amino acid substitutions including 80 in ≥ 10 genomes were detected in genotype A relatively to a 2004 reference. H512R in spike, first detected in 2009 and reported as involved in antibody neutralization, was found in all genotype A, almost always with V387I and K478N, and was predicted here to significantly improve cellular TMPRSS2 protein binding. Also, 1802 amino acid substitutions including 64 in ≥ 10 genomes were detected in genotype B relatively to a 2005 reference. This study substantially expands the global set of HCoV-HKU1 genomes. Genomics with protein structural analyses contributed to our understanding of HCoV-HKU1 evolution.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70217","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Umbilical Cord Mesenchymal Stem Cells Could Reduce Lung Damage Caused by H1N1 Influenza Virus Infection","authors":"Chun Yan Guo,&nbsp;Yi Wang,&nbsp;Qing Feng,&nbsp;Li Jun Sun,&nbsp;Yang Meng Feng,&nbsp;Yi Han Dong,&nbsp;Cui Xiang Xu","doi":"10.1002/jmv.70214","DOIUrl":"https://doi.org/10.1002/jmv.70214","url":null,"abstract":"<div>\u0000 \u0000 <p>Influenza A virus infection can cause acute respiratory distress syndrome (ARDS), and to date, viral pneumonia has been the main cause of ARDS. Bone marrow mesenchymal stem cells have shown promise for treating lung injury caused by avian influenza virus infection. At present, studies of the use of other stem cell types to treat human influenza virus-mediated lung damage are sparse. We assessed the use of umbilical cord mesenchymal stem cells (UC-MSCs) to treat damage from serious H1N1 influenza virus infections in cell and animal-based experiments. Maximum viral titers, inflammatory factor expression levels, differential expression of alveolar cell-related proteins, animal weight and survival rate, lung histopathology, and other indicators were evaluated. Compared with the control group, in cellular experiments, UC-MSCs could effectively inhibit H1N1 influenza viral replication and repair damaged host cells. In animal experiments, UC-MSCs reduced expression of pro-inflammatory cytokines, reduced entry of inflammatory cells into the lungs, alleviated lung inflammation, significantly reduced the extent of lung injury in mice, and improved lung histopathology, improving overall survival. A positive role of umbilical cord-derived mesenchymal stem cells in treating lung injury caused by H1N1 influenza virus infection that is worthy of clinical promotion has been demonstrated.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral Ad5 Vector-Based SARS-CoV-2 Vaccine Effectively Induces Mucosal and Systemic Immune Responses in BALB/c Mice","authors":"Tongyao Mao,&nbsp;Peng Zhang,&nbsp;Surui Jiang,&nbsp;Dandi Li,&nbsp;Jinsong Li,&nbsp;Qing Zhang,&nbsp;Hong Wang,&nbsp;Xiangyu Kong,&nbsp;Zhaojun Duan","doi":"10.1002/jmv.70236","DOIUrl":"https://doi.org/10.1002/jmv.70236","url":null,"abstract":"<div>\u0000 \u0000 <p>Mucosal immunity is essential for preventing viral infections through the mucosal route. The emerging SARS-CoV-2 variants have posed additional hurdles to the efficiency of existing vaccines. The rapid development of novel vaccines that generate broad mucosal and systemic immunity could be the most effective strategy to address this issue. In this study, we developed a recombinant and replication-deficient type-5 adenoviral vaccine with a built-in double-strand RNA adjuvant and the vaccine expresses the SARS-CoV-2 Omicron BA.1 spike (S) antigen (hereinafter referred to as “the oral vaccine”). We found that two doses of the oral vaccine in BALB/c mice generated long-lasting S-specific mucosal and systemic immune responses, as well as broad neutralizing antibodies and SIgA antibodies. In addition, we found that compared to an mRNA vaccine booster, using the oral vaccine as a booster could induce both effective mucosal and systemic immunity, addressing the limitation of mRNA vaccines in eliciting mucosal immunity. Prospective oral vaccines require further investigation into development and potential applications, particularly viral challenge experiments, before clinical trials.</p></div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-World Effectiveness of Sotrovimab in Patients Infected With SARS-CoV-2 Omicron Subvariant BA.2 in Western Sydney, Australia","authors":"Eric Kalo,&nbsp;Ziad Basyouni,&nbsp;Gideon Meyerowitz Katz,&nbsp;Vahid Karkvandi,&nbsp;Leanne Watson,&nbsp;Helen Crowther,&nbsp;Scott Read,&nbsp;Matthew V. N. O'Sullivan,&nbsp;Jasmin Ellis,&nbsp;Joanne Medlin,&nbsp;Golo Ahlenstiel","doi":"10.1002/jmv.70235","DOIUrl":"https://doi.org/10.1002/jmv.70235","url":null,"abstract":"<p>Laboratory-based findings suggest that Sotrovimab is significantly less effective against emerging CARS-CoV-2 variants, however, clinical data is lacking. Here we examined the effectiveness of sotrovimab, in preventing emergency department (ED) presentation and subsequent hospitalization in high-risk subgroups of patients during the SARS-CoV-2 Delta and Omicron waves in Western Sydney, Australia (<i>n</i> = 515). Risk for ED attendance was comparable in Omicron patients, whether BA.1 or BA.2, compared to Delta patients (hazard ratio of 0.97 [0.36–2.64]). These findings highlight the need for caution when using in vitro findings to drive clinical practice, especially when the consequence is to withhold potentially lifesaving treatment.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70235","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inflammatory Burden Index as a Predictor of In-Hospital Mortality in Patients With Severe Fever With Thrombocytopenia Syndrome","authors":"Zhixian Yang,&nbsp;Luonan Wang,&nbsp;Baoyu Hong,&nbsp;Zhicheng He,&nbsp;Qixing Zhang,&nbsp;Tingting Shen,&nbsp;Junjie Shen,&nbsp;Shichun Shen,&nbsp;Yan Cheng,&nbsp;Chen Gong","doi":"10.1002/jmv.70225","DOIUrl":"https://doi.org/10.1002/jmv.70225","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>Severe fever with thrombocytopenia syndrome (SFTS) is an infectious disease caused by a novel bunyavirus that poses a significant threat to human health. The aim of this study was to identify a precise and user-friendly indicator for predicting the mortality of patients with SFTS. We retrospectively analyzed data from 181 hospitalized patients with SFTS. Inflammatory markers, including the neutrophil-to-lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR), platelet-to-lymphocyte ratio (PLR), systemic inflammation index (SII), systemic inflammatory response index (SIRI), C-reactive protein-to-albumin ratio (CAR), and inflammatory burden index (IBI), were compared between the survival group and the nonsurvival group. A receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of the IBI for the poor prognosis of SFTS patients. Survival analysis was performed using the Kaplan‒Meier (KM) method. Univariate and multivariate Cox regression models were used to explore factors influencing the prognosis of hospitalized patients with SFTS. The results indicate that patients with high IBI had significantly higher mortality rates than those with low IBI. ROC curve analysis revealed that the IBI had better predictive value than the other indicators did, with an optimal cutoff value of 0.878. Kaplan–Meier survival analysis revealed that patients with high IBI had higher mortality rates and shorter survival times. Multivariate Cox regression analysis demonstrated that the IBI was an independent risk factor for poor prognosis in patients with SFTS. Therefore, the IBI can be used to help clinicians identify high-risk individuals and implement timely therapeutic interventions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}