Journal of Medical Virology最新文献

{"title":"Outbreak of Measles in Southern Switzerland: Is It Time to Put Stricter Rules on Vaccination?","authors":"Marco Bongiovanni, Alexandra Nobile, Giorgio Merlani, Niccolò Ramponi, Paola Bellini, Enos Bernasconi","doi":"10.1002/jmv.70244","DOIUrl":"https://doi.org/10.1002/jmv.70244","url":null,"abstract":"","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatitis C Virus Saint Petersburg Variant Detection With Machine Learning Methods","authors":"Nurhan Arslan,&nbsp;Bernhard Reuter,&nbsp;Joachim Buech,&nbsp;Thomas Lengauer,&nbsp;Martin Obermeier,&nbsp;Rolf Kaiser,&nbsp;Nico Pfeifer","doi":"10.1002/jmv.70169","DOIUrl":"https://doi.org/10.1002/jmv.70169","url":null,"abstract":"<p>Hepatitis C virus infection is a significant global health concern, affecting millions worldwide. Although direct-acting antivirals achieve over 90% success rate, treatment failures still occur, particularly when pan-genotypic DAAs are unavailable, and drugs need to be chosen based on the present HCV genotype. Genotyping tests can be misleading, especially in cases involving the 2k/1b recombinant variant. The 2k/1b variant was first discovered in Saint Petersburg in 2002 and is most commonly observed in Eastern European countries, including Russia, Georgia, and Ukraine. Due to migration, the 2k/1b variant has spread to Western Europe and other regions, potentially increasing HCV transmission and changing the virus's epidemiological landscape. The situation highlights the importance of molecular epidemiology in monitoring the spread of the 2k/1b variant. Accurate detection and characterization of the 2k/1b variant are crucial for an effective treatment if no pan-genotypic DAAs are available. To address this need, machine learning models were developed to predict the 2k/1b variant based on 1b and 2k/1b sequence data from nonstructural proteins. They were integrated into the geno2pheno_HCV tool, providing physicians and researchers with an open-access resource for determining HCV genotypes, including the 2k/1b variant.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70169","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epstein-Barr Virus-Associated Pneumonia in a Healthy Adult Presenting With Pulmonary Nodules and Deep Vein Thrombosis: A Case Report and Literature Review","authors":"Payam Tabarsi,&nbsp;Marjan Hemmatian,&nbsp;Maryam Moradi","doi":"10.1002/jmv.70226","DOIUrl":"https://doi.org/10.1002/jmv.70226","url":null,"abstract":"<div>\u0000 \u0000 <p>Epstein-Barr virus (EBV) is a common herpesvirus that affects more than 90% of people worldwide. It is usually spread through saliva. Although most infections show no symptoms or only lead to mono, uncommon complications such as respiratory issues can arise, particularly in individuals with weakened immune systems. While rare, cases of EBV-related pneumonia, lung nodules, and venous thromboembolism have been occasionally reported. A 17-year-old male came in with fever, dyspnea, massive hemoptysis, and sore throat. Numerous pulmonary nodules were shown in imaging. According to broncho-alveolar lavage, and biopsy results EBV-induced pneumonia was diagnosed. During hospitalization, the patient also experienced deep vein thrombosis and needed heparin treatment. The current study contributes to the increasing amount of research on unusual displays of EBV in healthy adults. It highlights the significance of considering EBV in the differential diagnosis for healthy adults with unexplained pulmonary nodules and venous thromboembolism.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Measles Virus Genotype D8 in Wastewater of the Brussels Capital Region, Belgium","authors":"Annabel Rector,&nbsp;Mandy Bloemen,&nbsp;Bart Hoorelbeke,&nbsp;Marc Van Ranst,&nbsp;Elke Wollants","doi":"10.1002/jmv.70251","DOIUrl":"https://doi.org/10.1002/jmv.70251","url":null,"abstract":"<p>We analyzed wastewater from Belgian treatment plants to look for measles virus. Genotype D8 was identified in Brussels North samples, matching sequences from 15 regional measles cases. Finding measles virus in wastewater can suggest undetected virus transmission. Wastewater surveillance is a valuable tool for identifying viral circulation and supporting public health interventions against outbreaks.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70251","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Polo-Like Kinase 1 Dampens the Replication of Vaccinia Virus in Mammalian Cells","authors":"Junda Zhu,&nbsp;Xuejiao Jia,&nbsp;Shuning Ren,&nbsp;Zihui Zhang,&nbsp;Hua Li,&nbsp;Jing Wang,&nbsp;Baifen Song,&nbsp;Wenxue Wu,&nbsp;Chen Peng","doi":"10.1002/jmv.70240","DOIUrl":"https://doi.org/10.1002/jmv.70240","url":null,"abstract":"<div>\u0000 \u0000 <p>Since the eradication of smallpox, zoonotic poxviruses, such as the mpox virus (MPXV), continue to pose a threat to public health. Identifying drugs that reduce poxvirus infection and replication, as well as understanding their molecular mechanisms, is essential for epidemic control. Polo-like kinase 1 (PLK1) has been shown to facilitate vaccinia virus (VACV) infection and replication. This study confirms the effects of the PLK1 inhibitors HMN-214 and ON-01910 on VACV replication in A549 cells. Both viral titers and DNA loads were significantly reduced in treated cells after infection. Additionally, ON-01910 demonstrated broad-spectrum antiviral activity against the lumpy skin disease virus (LSDV) and the infectious bovine rhinotracheitis virus (IBRV) in vitro. PLK1 knockdown in A549 cells also led to a reduction in VACV protein expression, viral titers, and DNA levels. Further analysis showed that VACV infection leads to the accumulation of PLK1 near viral factories. However, despite its strong in vitro effects, ON-01910 did not significantly reduce VACV replication in mice. These findings highlight the critical role of PLK1 in VACV replication and its potential as a target for antiviral therapy against orthopoxviruses.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiological Investigations for Chikungunya Virus in Children With Acute Encephalitis Syndrome in a Non-Outbreak Setting in Southern India","authors":"Tina Damodar,&nbsp;Chitra Pattabiraman,&nbsp;Bhagteshwar Singh,&nbsp;Maria Jose,&nbsp;Namratha Prabhu,&nbsp;Akhila L,&nbsp;Pramada Prasad,&nbsp;Uddhava V. Kinhal,&nbsp;A. V. Lalitha,&nbsp;Fulton Sebastian Dsouza,&nbsp;Sushma Veeranna Sajjan,&nbsp;Vykuntaraju K. Gowda,&nbsp;Vasanthapuram Ravi,&nbsp;Ruwanthi Kolamunnage-Dona,&nbsp;Benedict D. Michael,&nbsp;Tom Solomon,&nbsp;Ravi Yadav,&nbsp;Lance Turtle","doi":"10.1002/jmv.70233","DOIUrl":"https://doi.org/10.1002/jmv.70233","url":null,"abstract":"<p>Chikungunya virus (CHIKV) is an emerging cause of acute encephalitis syndrome (AES) in India, with limited data on its role in childhood AES in southern India. We systematically evaluated children with AES in southern India during a non-epidemic period for CHIKV. Serum and cerebrospinal fluid (CSF) samples were tested for CHIKV using IgM ELISA and real-time reverse transcriptase PCR. Amplicon sequencing was performed on PCR-positive samples. Clinical and laboratory features were compared between children with and without CSF CHIKV positivity (PCR/IgM antibodies). Of 376 children with AES, 20 (5.3%) had positive CHIKV tests. Co-infections were common, particularly with scrub typhus. Children presented with diverse symptoms affecting various organ systems. Neurological manifestations included meningism, seizures, cerebellar signs, behavioral abnormalities, cranial nerve involvement, involuntary movements, and hemiparesis/hemiplegia. Children with CSF CHIKV positivity showed more focal neurological deficits and transaminitis, and less musculoskeletal symptoms. Sequencing confirmation of CHIKV was made in all patients with positive CHIKV PCR, revealing a close relationship with 2016 Kenyan and Indian strains, albeit in a different clade within the East/Central/South African genotype. Along with important mutations known to impact CHIKV infectivity, four novel amino acid substitutions were detected in envelope protein coding regions. Our findings underscore the importance of routine and comprehensive CHIKV testing for children with AES, irrespective of season/outbreak. The high rate of co-infections warrants further research. Continued genomic surveillance is essential to monitor emerging mutations with epidemic potential, increased severity and the risk of neurological disease.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70233","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143423548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RT-RPA Assisted CRISPR/Cas12a Based One-Pot Rapid and Visual Detection of the Pan-Dengue Virus","authors":"Pooja Bhardwaj,&nbsp;Preeti Dhangur,&nbsp;Alagarasu Kalichamy,&nbsp;Rajeev Singh","doi":"10.1002/jmv.70219","DOIUrl":"https://doi.org/10.1002/jmv.70219","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>Globally ≤ 4 billion of the population are at potential risk of contracting dengue virus (DENV) infection. Seasonal outbreaks of dengue are frequently reported causing a high healthcare burden. Undiagnosed DENV can lead to severe morbidity and mortality. Early diagnosis of DENV relies on molecular methods, which are impractical in resource-constrained settings (RCSs). Dengue can be caused by any of the four distinct DENV serotypes. Therefore, a simple method for rapid diagnosis of Pan-DENV serotypes is of utmost importance at RCSs. A fluorescence detection platform for Pan-DENV using RT-RPA and CRISPR/Cas12a was developed targeting nonstructural 1 (<i>NS1</i>) gene for DENV-1, 2, and 3, and envelope (<i>E</i>) gene for DENV-2. Further, crRNA specific to DENV serotypes were designed to facilitate CRISPR/Cas12a detection. Analytical sensitivity was determined using synthetic RNA and DENV serotypes genome. Clinical validation of the assay was performed using RNA extracted from AES/AFI clinical samples. The developed CRISPR/Cas12a-based detection platform can detect all four serotypes of DENV viz 1−4 in a single pot using fluorescence detection. This assay showed the limit of detection ≥ 781 zg reaction⁻¹, ≥ 1.81 ag reaction⁻¹, ≥ 62.5 fg reaction⁻¹, and ≥ 2.5 pg reaction⁻¹ for synthetic DENV-1, DENV-2, DENV-3, and DENV-4 template, respectively. Our assay demonstrated the analytic sensitivity of ≥ 10 ng reaction⁻¹ for DENV-1 and DENV-4, and ≥ 0.5 ng reaction⁻¹ for DENV-3 and DENV-4 genomes. This assay showed no cross-reactivity with other related etiologies tested causing AFI/AES. With 76 clinical samples (DENV PCR positive = 16, DENV PCR negative = 60), the assay demonstrated 93.7% sensitivity and 100% specificity with an overall accuracy of 98.7% for detection of the Pan-DENV serotypes. Our assay displayed comparable results to that of RT-PCR. The ease of interpretation and rapid detection of the Pan-DENV, represents the potential of the developed assay as an ideal point-of-care test. This assay upon field-deployment could help in reducing healthcare burden, provide differential diagnosis and support initiating early and prompt treatment to patients at RCS.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor Necrosis Factor-Alpha Inhibits the Replication of Patient-Derived Archetype BK Polyomavirus While Activating Rearranged Strains","authors":"Lise Lauterbach-Rivière,&nbsp;Lucia Thuringer,&nbsp;Pascal Feld,&nbsp;Lina Kathrin Toews,&nbsp;Jessica Schüssler,&nbsp;Jonas Klinz,&nbsp;Lars Gläser,&nbsp;Stefan Lohse,&nbsp;Anna Sternjakob,&nbsp;Gilles Gasparoni,&nbsp;Kathrin Kattler-Lackes,&nbsp;Jörn Walter,&nbsp;Marcel A. Lauterbach,&nbsp;Sven Rahmann,&nbsp;Lars Möller,&nbsp;Michael Laue,&nbsp;Martin Janssen,&nbsp;Michael Stöckle,&nbsp;David Schmit,&nbsp;Danilo Fliser,&nbsp;Sigrun Smola","doi":"10.1002/jmv.70210","DOIUrl":"https://doi.org/10.1002/jmv.70210","url":null,"abstract":"<p>To date, no drugs are approved for BK polyomavirus (BKPyV) reactivation, a major cause of nephropathy after kidney transplantation. Recently, tumor necrosis factor-α (TNF-α) blockade has been proposed as a promising therapy, however, the effect of TNF-α on the clinically most common archetype (ww) BKPyV remained unclear. Assays in primary renal proximal tubule epithelial cells (RPTEC) allowed efficient replication only of BKPyV strains with rearranged (rr) non-coding control regions (NCCR), which may develop at later disease stages, but not of ww-BKPyV. Here, we optimized culture conditions allowing robust replication of patient-derived ww-BKPyV, while efficiently preserving their ww-NCCR. TNF-α promoted rr-BKPyV replication, while the T_H1 cytokine IFN-γ suppressed it, also in the presence of TNF-α. Surprisingly, TNF-α alone was sufficient to suppress all ww-BKPyV strains tested. Comprehensive analysis using siRNAs, and chimeric or mutated BKPyV-strains revealed that the response to TNF-α depends on the NCCR type, and that the NF-κB p65 pathway but not the conserved NF-κB binding site is essential for the TNF-α-induced enhancement of rr-BKPyV replication. Our data suggest that in immunosuppressed patients with archetype-dominated infections, TNF-α blockade could interfere with natural TNF-α-mediated anti-BKPyviral control, and this could be detrimental when IFN-γ-driven T_H1 responses are impaired. Ongoing inflammation, however, could lead to the selection of rearrangements responding to NCCR-activating pathways downstream of NF-κB p65 signaling, that may overcome the initial TNF-α-mediated suppression. Our findings also highlight the importance of using clinically relevant BKPyV isolates for drug testing and discovery, for which this new assay paves the way.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70210","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Five-Year (2017–2022) Evolutionary Dynamics of Human Coronavirus HKU1 in Southern France With Emergence of Viruses Harboring Spike H512R Substitution","authors":"Houmadi Hikmat,&nbsp;Lorlane Le Targa,&nbsp;Céline Boschi,&nbsp;Justine Py,&nbsp;Aurélie Morand,&nbsp;Jean-Christophe Lagier,&nbsp;Sarah Aherfi,&nbsp;Jacques Fantini,&nbsp;Bernard La Scola,&nbsp;Philippe Colson","doi":"10.1002/jmv.70217","DOIUrl":"https://doi.org/10.1002/jmv.70217","url":null,"abstract":"<p>HCoV-HKU1 diversity and evolution were scarcely studied. We performed next-generation sequencing (NGS) and analysis of HCoV-HKU1 genomes over 5 years. NGS used Illumina technology on NovaSeq 6000 following whole genome PCR amplification by an in-house set of primers designed using Gemi and PrimalScheme. Genome assembly and analyses used CLC Genomics, Mafft, BioEdit, Nextstrain, Nextclade, MEGA, and iTol bioinformatic tools. Spike molecular modeling and dynamics simulations used Molegro Molecular Viewer and Hyperchem programs. Twenty-eight PCR systems allowed obtaining 158 HCoV-HKU1 genomes including 69 and 89 of genotypes A and B, respectively. Both genotypes co-circulated during the study period but one predominated each year. A total of 1683 amino acid substitutions including 80 in ≥ 10 genomes were detected in genotype A relatively to a 2004 reference. H512R in spike, first detected in 2009 and reported as involved in antibody neutralization, was found in all genotype A, almost always with V387I and K478N, and was predicted here to significantly improve cellular TMPRSS2 protein binding. Also, 1802 amino acid substitutions including 64 in ≥ 10 genomes were detected in genotype B relatively to a 2005 reference. This study substantially expands the global set of HCoV-HKU1 genomes. Genomics with protein structural analyses contributed to our understanding of HCoV-HKU1 evolution.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70217","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Umbilical Cord Mesenchymal Stem Cells Could Reduce Lung Damage Caused by H1N1 Influenza Virus Infection","authors":"Chun Yan Guo,&nbsp;Yi Wang,&nbsp;Qing Feng,&nbsp;Li Jun Sun,&nbsp;Yang Meng Feng,&nbsp;Yi Han Dong,&nbsp;Cui Xiang Xu","doi":"10.1002/jmv.70214","DOIUrl":"https://doi.org/10.1002/jmv.70214","url":null,"abstract":"<div>\u0000 \u0000 <p>Influenza A virus infection can cause acute respiratory distress syndrome (ARDS), and to date, viral pneumonia has been the main cause of ARDS. Bone marrow mesenchymal stem cells have shown promise for treating lung injury caused by avian influenza virus infection. At present, studies of the use of other stem cell types to treat human influenza virus-mediated lung damage are sparse. We assessed the use of umbilical cord mesenchymal stem cells (UC-MSCs) to treat damage from serious H1N1 influenza virus infections in cell and animal-based experiments. Maximum viral titers, inflammatory factor expression levels, differential expression of alveolar cell-related proteins, animal weight and survival rate, lung histopathology, and other indicators were evaluated. Compared with the control group, in cellular experiments, UC-MSCs could effectively inhibit H1N1 influenza viral replication and repair damaged host cells. In animal experiments, UC-MSCs reduced expression of pro-inflammatory cytokines, reduced entry of inflammatory cells into the lungs, alleviated lung inflammation, significantly reduced the extent of lung injury in mice, and improved lung histopathology, improving overall survival. A positive role of umbilical cord-derived mesenchymal stem cells in treating lung injury caused by H1N1 influenza virus infection that is worthy of clinical promotion has been demonstrated.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 2","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}