Journal of Medical Virology最新文献

{"title":"Monkeypox Virus and Pregnancy","authors":"Jonatane Andrieu,&nbsp;Jean-louis Mège,&nbsp;Soraya Mezouar","doi":"10.1002/jmv.70337","DOIUrl":"https://doi.org/10.1002/jmv.70337","url":null,"abstract":"<p>Human monkeypox (Mpox) is a zoonotic disease caused by monkeypox virus (MPXV) present in western Africa and exported sporadically worldwide. MPXV causes illness in individuals and pregnant women which constitute a population at risk with obstetrical and fetal complications including miscarriage, stillbirth and premature delivery. There are accumulated data suggesting a vertical transmission of MPXV from mother to fetus. This review provides an overview of the literature on MPXV infection in pregnant women with a specific focus on vertical transmission.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 4","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70337","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143827032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Emerging Roles of Alternative Splicing in Human Oncovirus Infection","authors":"Xuefei Liao,&nbsp;Li Yang,&nbsp;Mingjuan Jiang,&nbsp;Yujie Xin,&nbsp;Huirong Yan,&nbsp;Qingshuang Qin,&nbsp;Mengdi Chen,&nbsp;Jianhong Lu","doi":"10.1002/jmv.70346","DOIUrl":"https://doi.org/10.1002/jmv.70346","url":null,"abstract":"<div>\u0000 \u0000 <p>Alternative splicing (AS) is one of the most potent mechanisms for expanding the diversity of proteomes. During infection, human oncogenic viruses may exploit the AS to facilitate their replication cycle. Moreover, persistently infecting viruses can target key genes involved in classical signaling pathways to promote viral persistence and tumor progression. Here, we highlight how oncogenic viruses hijack AS system to manipulate host biological processes, and the host's AS system in turn modulates viral infection and replication. In addition, we have summarized the relatively underexplored involvement of noncoding RNAs in AS following tumor virus infection. This bidirectional interaction provides novel insights into interaction of virus-host and opens new avenues for therapeutic strategies targeting oncogenic viral infections.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 4","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143827033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-Term Stroke and Mortality Risk Reduction Associated With Acute-Phase Paxlovid Use in Mild-to-Moderate COVID-19","authors":"Cheng-Hsun Chuang,&nbsp;Yu-Hsun Wang,&nbsp;Liang-Tsai Yeh,&nbsp;Chao-Bin Yeh","doi":"10.1002/jmv.70351","DOIUrl":"https://doi.org/10.1002/jmv.70351","url":null,"abstract":"<div>\u0000 \u0000 <p>This retrospective cohort study investigated whether Paxlovid (nirmatrelvir/ritonavir) use during the acute phase of mild-to-moderate COVID-19 reduces the risk of ischemic or hemorrhagic stroke occurring more than 3 months post-diagnosis, a condition classified as long COVID. Utilizing TriNetX electronic health records comprising 118 million patients in the United States, adults aged 18 years or older with confirmed COVID-19 diagnoses from 2022 to 2023 were categorized into Paxlovid (administered within 5 days of diagnosis) and non-Paxlovid groups. Exclusion criteria included prior cerebrovascular disease, mortality within 3 months, use of specific antivirals, and severe clinical conditions such as ICU admission, intubation, mechanical support, SpO₂ &lt; 90%, respiratory rate &gt; 30/min, sepsis, systemic inflammatory response syndrome, and acute respiratory distress syndrome. The index date was the initial COVID-19 diagnosis. Propensity score matching in a 1:1 ratio controlled for confounding factors, and stroke (ischemic or hemorrhagic) and mortality were analyzed using Kaplan–Meier survival curves and Cox proportional hazards models. Among 181 992 matched pairs, Paxlovid use was associated with a significantly reduced risk of ischemic and hemorrhagic stroke (hazard ratio [HR] 0.85; 95% confidence interval [CI]: 0.80–0.89) and all-cause mortality (HR 0.68; 95% CI: 0.63–0.73) during the long COVID period, defined as more than 90 days post-diagnosis. Subgroup analyses demonstrated consistent protective effects across age, sex, BMI, comorbidities such as hypertension, diabetes, and hyperlipidemia, and vaccination status. Notably, older adults (HR 0.81; 95% CI: 0.76–0.86) and individuals with metabolic conditions, including obesity (HR 0.86; 95% CI: 0.78–0.96), exhibited pronounced benefits, and the protective effects were observed irrespective of vaccination status. These findings highlight that Paxlovid use during the acute phase of COVID-19 significantly reduces the risk of long-term cerebrovascular events and mortality, emphasizing its critical role in mitigating long-term complications associated with COVID-19.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 4","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143827035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HBcrAg Dynamic Change During Treatment Predicts HBsAg Loss in Pediatric Patients With Chronic Hepatitis B","authors":"Ling Ye,&nbsp;Wenxian Ouyang,&nbsp;Yingping Gu,&nbsp;Zhenzhen Yao,&nbsp;Xin Lai,&nbsp;Sisi Li,&nbsp;Meng Yang,&nbsp;Songxu Peng","doi":"10.1002/jmv.70350","DOIUrl":"https://doi.org/10.1002/jmv.70350","url":null,"abstract":"<div>\u0000 \u0000 <p>In children with chronic hepatitis B(CHB), HBsAg loss is a critical indicator of functional cure. However, little is known about the correlation between the kinetics of HBcrAg and HBsAg loss in the pediatric population. The aim of this study was to investigate whether the HBcrAg change in children CHB patients receiving antiviral therapy is associated with the occurrence of HBsAg loss. A prospective cohort study was conducted on 86 pediatric patients with treatment-naive CHB. The speed of HBcrAg change was calculated during the treatment period, and the patients were divided into two groups (rapid decline, slow decline) according to the median speed (0.0221 log₁₀ U/mL/week). The primary outcome is the serological HBsAg loss in CHB children after antiviral therapy. In 86 participants, 26 (30.2%) achieved HBsAg loss at the end of follow-up. Baseline HBsAg levels (HR = 0.36, 95% CI: 0.19–0.68) and the speed of HBcrAg decline during treatment (HR = 1.30, 95% CI: 1.10–1.40) were found to be independent predictors of the occurrence of HBsAg loss. The Kaplan–Meier curve showed that the cumulative incidence of HBsAg loss in the rapid decline group was significantly higher than that in the slow decline group. Also, receiver operating characteristic curve showed that the speed of HBcrAg decline could effectively predict the occurrence of HBsAg loss (AUC: 0.735, <i>p</i> &lt; 0.001). Moreover, when combining the baseline HBsAg levels for prediction, the AUC increased to 78.4%. The speed of HBcrAg decline during antiviral therapy was significantly associated with HBsAg loss in pediatric CHB patients.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 4","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143818755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Changed Endemic Pattern of Respiratory Syncytial Virus Infections in Pediatric Patients With the Relaxation of NPIs in Beijing, China, 2022−2023","authors":"Mingli Jiang,&nbsp;Fengjie Wang,&nbsp;Yanpeng Xu,&nbsp;Zhenzhi Han,&nbsp;Qi Guo,&nbsp;Yu Sun,&nbsp;Runan Zhu,&nbsp;Dongmei Chen,&nbsp;Yutong Zhou,&nbsp;Dong Qu,&nbsp;Ling Cao,&nbsp;Wei Qu,&nbsp;Fengmin Lu,&nbsp;Linqing Zhao","doi":"10.1002/jmv.70348","DOIUrl":"https://doi.org/10.1002/jmv.70348","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>With the cancellation of non-pharmaceutical interventions (NPIs) since December 26, 2022, in Beijing, it is essential to update the endemic pattern of respiratory syncytial virus (RSV) in pediatric patients. Respiratory specimens were collected from hospitalized children with acute respiratory infections (ARIs) from January 2022 to December 2023 in Beijing for multiple pathogen screening. Then, specimens positive for RSV were subtyped by PCR and genotyped by <i>G</i> gene sequencing and phylogenetic analysis with Mega X. The clinical data of children only positive for RSV were compared using SPSS 22.0 software. Among 7131 specimens enrolled, there were 9.21% (203/2205) and 10.74% (529/4926) positive for RSV before and after the cancellation of NPIs, respectively. The expected RSV endemic season from November 2022 to March 2023 disappeared, and the RSV positive rates kept in 0.00% in January and February 2023, which were then increased rapidly to 14.81% in April and 24.60% in May, and to 14.35% and 18.18% again in November and December 2023, with the dominant subtype of RSV transferred from A to B in November 2023. Phylogenetic analysis revealed that clusters ON1.2 and BA9.3, especially, a new variant RSV-B-BA9-954bp, only showed in specimens collected after the cancellation of NPIs. Higher proportion of children aged 3−6 years and over 6 years which increased from 10.56% to 21.41%, and from 3.33% to 7.59%, respectively, and less severe pneumonia cases which decreased from 50.00% to 17.86%, were observed after the cancellation of NPIs. With the cancellation of NPIs, a delayed endemic season of RSV was shown in April and May 2023, with new clusters of ON1.2 and BA9.3, especially a new variant (RSV-B-BA9-954bp), a high proportion of children aged 3−6 years and over 6 years, and less severe pneumonia cases.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 4","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143818756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seropositivity to Dengue, Zika, Yellow Fever, and West Nile Viruses in Senegal, West Africa","authors":"Sebastian Gallon,&nbsp;Mouhamad Sy,&nbsp;Prince Baffour Tonto,&nbsp;Ibrahima Mbaye Ndiaye,&nbsp;Mariama Toure,&nbsp;Amy Gaye,&nbsp;Mariama Aidara,&nbsp;Amadou Moctar Mbaye,&nbsp;Abdoulaye Kane Dia,&nbsp;Mamadou Alpha Diallo,&nbsp;Jules Francois Gomis,&nbsp;Mamadou Samb Yade,&nbsp;Younous Diedhiou,&nbsp;Baba Dieye,&nbsp;Khadim Diongue,&nbsp;Mame Cheikh Seck,&nbsp;Aida S. Badiane,&nbsp;Daouda Ndiaye,&nbsp;Bobby Brooke Herrera","doi":"10.1002/jmv.70338","DOIUrl":"https://doi.org/10.1002/jmv.70338","url":null,"abstract":"<p>West Africa serves as a critical region for the co-circulation of mosquito-borne flaviviruses, which often precipitate sporadic outbreaks. This study investigated the seropositivity to dengue virus serotypes 1–4 (DENV-1–4), Zika virus (ZIKV), yellow fever virus (YFV), and West Nile virus (WNV) in three regions of Senegal: Sindia, Thies, and Kedougou. We retrospectively analyzed 470 serum samples for flavivirus immunoglobulin G (IgG) using a DENV-2 envelope (E) ELISA. Our findings revealed an overall flavivirus seroprevalence of 37.23%. Among the DENV-2 E IgG positive samples, the proportion of subjects with IgG to DENV-1–4, ZIKV, YFV, or WNV NS1 was 57.14%, 12.57%, 80.57%, and 17.14%, respectively, with 66.86% harboring neutralizing antibodies against two or more flaviviruses. We also identified that residents in Sindia (ZIKV, aOR, 9.428; 95% CI: 1.882–47.223 and WNV, aOR, 6.039; 95% CI: 1.855–19.658) and Kedougou (ZIKV, aOR, 7.487; 95% CI: 1.658–33.808 and WNV, aOR, 1.142; 95% CI: 0.412–3.164) were at significant risk for ZIKV and WNV exposure. This study underscores the complexity of flavivirus epidemiology in West Africa and the necessity for enhanced surveillance to inform public health strategies.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 4","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70338","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole Genome Sequencing and Genetic Diversity of Respiratory Viruses Detected in Children With Acute Respiratory Infections: A One-Year Cross-Sectional Study in Senegal","authors":"Anna Julienne Selbé Ndiaye,&nbsp;Sebastien Cortaderona,&nbsp;Léa Delorme,&nbsp;Mamadou Beye,&nbsp;Idir Kacel,&nbsp;Vincent Bossi,&nbsp;Gora Lo,&nbsp;Nafissatou Leye,&nbsp;Abdou Padane,&nbsp;Halimatou Diop-Ndiaye,&nbsp;Coumba Touré Kane,&nbsp;Ndèye Ramatoulaye Diagne,&nbsp;Cheikh Sokhna,&nbsp;Souleymane Mboup,&nbsp;Pierre-Edouard Fournier","doi":"10.1002/jmv.70342","DOIUrl":"https://doi.org/10.1002/jmv.70342","url":null,"abstract":"<p>Acute respiratory infections (ARI) are a health priority, especially in countries with limited resources. They are a major cause of morbidity and mortality, especially among children and the elderly. In Senegal, the endemic circulation of respiratory viruses other than influenza has been demonstrated. However, there is a paucity of data exploring the genetic diversity of these viruses based on whole-genome sequencing. In this study, we present data on the genetic diversity of respiratory viruses in children under 15 years old in Senegal, including an overview of the different pathogens detected. Between November 2022 and November 2023, we collected nasopharyngeal swabs from children seen in curative consultations for symptoms of acute respiratory infections. Of the 156 children included, 73.7% tested positive for at least one pathogen. The most frequently detected virus was rhinovirus (50.0%), followed by influenza B (41.6%) and human parainfluenza virus type 3 (7.6%). Combinations of rhinovirus/influenza B, human parainfluenza virus type 2/human parainfluenza virus type 4, and rhinovirus/influenza B/adenovirus were the most frequently identified. A statistically significant association was detected between some of the viruses detected. A high genetic diversity of respiratory viruses circulating in children was revealed. The strains were phylogenetically close to various strains circulating worldwide, suggesting a global circulation of respiratory viruses. Our study provides the first complete genome sequences of human parainfluenza viruses type 2, 3, 4 and human bocavirus from Senegal and thus contributes to the enrichment of international databases on sequences from Senegal and underlines the importance of sequencing in the dynamics of pathogen circulation.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 4","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70342","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemic Outbreak of Respiratory Syncytial Virus Infection After the end of the Zero-COVID-19 Policy in China: Molecular Characterization and Disease Severity Associated With a Novel RSV-B Clade","authors":"Yulin Zhang,&nbsp;Dongya Pu,&nbsp;Qi Liu,&nbsp;Binbin Li,&nbsp;Binghuai Lu,&nbsp;Bin Cao","doi":"10.1002/jmv.70343","DOIUrl":"https://doi.org/10.1002/jmv.70343","url":null,"abstract":"<div>\u0000 \u0000 <p>The resurgence of respiratory syncytial virus (RSV) has become a major concern recently. This study aimed to describe the temporal dynamics, genotype variability and disease severity of RSV infection after the end of the Zero-COVID-19 policy in Beijing, China. A total of 905 patients were positive for RSV at National Center for Respiratory Medicine in Beijing from November 2019 to April 2024. Of these, 238 positive samples from different patients were successfully sequenced, 96 of which were identified as the RSV-A and 142 as the RSV-B. Phylogenetic analyses were performed to investigate genetic diversity. The first surge of RSV infection after the end of the Zero-COVID-19 policy was quite intense and occurred out of season, mainly affecting children. The subsequent RSV outbreak had a significant impact among adults. RSV cases were caused by various clades and a new clade B.D.E.1 was identified as the main cause of the epidemic outbreak in adults. Pneumonia in immunocompromised hosts caused by clade B.D.E.1 was more common compared to other clades. Accumulation of substitutions in clade B.D.E.1 could confer a fitness advantage in vivo. However, there was no statistical difference in clinical outcomes between patients infected by clade B.D.E.1 and those infected by other RSV clades. This study addressed the timing and trends of the RSV infections, focusing on epidemic outbreaks, molecular characterization, and disease severity associated with a novel clade B.D.E.1. A more effective prevention strategy for RSV infections in childhood, immunosuppressed adults and elderly might be warranted.</p></div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 4","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic Analysis of 442 Clinical Plasma Samples From Individuals With Symptom Records Revealed Subtypes of Convalescent Patients Who Had COVID-19","authors":"Jiangfeng Liu,&nbsp;Li Guo,&nbsp;Jingchuan Zhong,&nbsp;Yue Wu,&nbsp;Xinming Wang,&nbsp;Xiaoyue Tang,&nbsp;Kaiyuan Min,&nbsp;Yehong Yang,&nbsp;Wanjun Peng,&nbsp;Qiaochu Wang,&nbsp;Tao Ding,&nbsp;Xiaoying Gu,&nbsp;Hui Zhang,&nbsp;Ying Liu,&nbsp;Chaolin Huang,&nbsp;Bin Cao,&nbsp;Jianwei Wang,&nbsp;Lili Ren,&nbsp;Juntao Yang","doi":"10.1002/jmv.70203","DOIUrl":"https://doi.org/10.1002/jmv.70203","url":null,"abstract":"<p>After the coronavirus disease 2019 (COVID-19) pandemic, the postacute effects of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection have gradually attracted attention. To precisely evaluate the health status of convalescent patients with COVID-19, we analyzed symptom and proteome data of 442 plasma samples from healthy controls, hospitalized patients, and convalescent patients 6 or 12 months after SARS-CoV-2 infection. Symptoms analysis revealed distinct relationships in convalescent patients. Results of plasma protein expression levels showed that C1QA, C1QB, C2, CFH, CFHR1, and F10, which regulate the complement system and coagulation, remained highly expressed even at the 12-month follow-up compared with their levels in healthy individuals. By combining symptom and proteome data, 442 plasma samples were categorized into three subtypes: S1 (metabolism-healthy), S2 (COVID-19 retention), and S3 (long COVID). We speculated that convalescent patients reporting hair loss could have a better health status than those experiencing headaches and dyspnea. Compared to other convalescent patients, those reporting sleep disorders, appetite decrease, and muscle weakness may need more attention because they were classified into the S2 subtype, which had the most samples from hospitalized patients with COVID-19. Subtyping convalescent patients with COVID-19 may enable personalized treatments tailored to individual needs. This study provides valuable plasma proteomic datasets for further studies associated with long COVID.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 4","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70203","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenome-Based Characterization of the Gut Virome Signatures in Patients With Gout","authors":"Liansha Huang,&nbsp;Changming Chen,&nbsp;Jinxin Meng,&nbsp;Qiulong Yan,&nbsp;Guangbin Luo,&nbsp;Shanshan Sha,&nbsp;Yida Xing,&nbsp;Changyan Liu,&nbsp;Mingxi Xu,&nbsp;Lin Zhao,&nbsp;Shumin Guo,&nbsp;Xiaoling Wu,&nbsp;Huiling Chen,&nbsp;Jie Ma,&nbsp;Wei You,&nbsp;Yan Zhang,&nbsp;Ruochun Guo,&nbsp;Shenghui Li,&nbsp;Xueming Yao,&nbsp;Wukai Ma,&nbsp;Xiaodan Kong,&nbsp;Peng Zhou,&nbsp;Wen Sun","doi":"10.1002/jmv.70336","DOIUrl":"https://doi.org/10.1002/jmv.70336","url":null,"abstract":"<div>\u0000 \u0000 <p>The gut microbiome has been implicated in the development of autoimmune diseases, including gout. However, the role of the gut virome in gout pathogenesis remains underexplored. We employed a reference-dependent virome approach to analyze fecal metagenomic data from 102 gout patients (77 in the discovery cohort and 25 in the validation cohort) and 86 healthy controls (HCs) (63 and 23 in each cohort). A subset of gout patients in the discovery cohort provided longitudinal samples at Weeks 2, 4, and 24. Our analysis revealed significant alterations in the gut virome of gout patients, including reduced viral richness and shifts in viral family composition. Notably, <i>Siphoviridae</i>, <i>Myoviridae</i>, and <i>Podoviridae</i> were depleted, while <i>Quimbyviridae</i>, <i>Retroviridae</i>, and <i>Schitoviridae</i> were enriched in gout patients. We identified 359 viral operational taxonomic units (vOTUs) associated with gout. Enriched vOTUs in gout patients predominantly consisted of Fusobacteriaceae, Bacteroidaceae, and Selenomonadaceae phages, while control-enriched vOTUs included Ruminococcaceae, Oscillospiraceae, and Enterobacteriaceae phages. Longitudinal analysis revealed that a substantial proportion of these virome signatures remained stable over 6 months. Functional profiling highlighted the enrichment of viral auxiliary metabolic genes, suggesting potential metabolic interactions between viruses and host bacteria. Notably, gut virome signatures effectively discriminated gout patients from HCs, with high classification performance in the validation cohort. This study provides the first comprehensive characterization of the gut virome in gout, revealing its potential role in disease pathogenesis and highlighting virome-based signatures as promising biomarkers for gout diagnosis and future therapeutic strategies.</p></div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 4","pages":""},"PeriodicalIF":6.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}