Journal of Medical Virology最新文献

{"title":"Oncogenic Mechanisms of Kaposi's Sarcoma-Associated Herpesvirus on Cell Metabolism and Cell Transformation","authors":"Quanyuan Wan,&nbsp;Andrew J. Tucker,&nbsp;Jun Zhao","doi":"10.1002/jmv.70565","DOIUrl":"https://doi.org/10.1002/jmv.70565","url":null,"abstract":"<p>Kaposi's sarcoma-associated herpesvirus (KSHV) is a human double-stranded DNA virus that is responsible for the development of Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), multicentric Castleman's disease (MCD), and KSHV inflammatory cytokine syndrome (KICS). KSHV infection manipulates distinct cellular proteins and signaling pathways, resulting in immune escape, cell death inhibition, infinite cell growth, and cancer formation. Current treatments for KSHV-associated cancers are limited to conventional strategies targeting nonviral cancers which encounter limited efficacy and drug resistance. Understanding the molecular and cellular mechanisms underlying KSHV tumorigenesis is essential for the development of effective prevention and targeted therapeutics. In this review, we summarize recent studies and provide an updated understanding of the mechanisms employed by KSHV for metabolic reprogramming, cell immortalization, cell proliferation, and cell transition.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70565","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic High Epstein-Barr Viral Load Carriage Is Positively Correlated With Tacrolimus Intra-Patient Variability After Pediatric Liver Transplantation","authors":"Anrui Wang,&nbsp;Xiaoke Dai,&nbsp;Chenyu Yang,&nbsp;Bingqian Tan,&nbsp;Mingman Zhang","doi":"10.1002/jmv.70562","DOIUrl":"https://doi.org/10.1002/jmv.70562","url":null,"abstract":"<p>Chronic high Epstein-Barr virus (EBV) load (CHL) carriage has been closely associated with EBV infection after pediatric liver transplantation. Elevated tacrolimus (Tac) blood concentrations increased the risk of EBV-associated diseases. Tacrolimus intra-patient variability (Tac-IPV) help predict poor outcomes. This study examines whether Tac-IPV correlate with CHL carriage in living-donor-dominant pediatric liver transplantation. We analyzed the clinical data of 153 pediatric liver transplant recipients receiving Tac treatment with a 2-year follow-up period. Tac-IPV quantification as coefficient of variation (CV). CHL was defined as EBV DNA &gt; 16,000 copies/mL in whole blood or &gt; 200 copies/10⁵ peripheral blood mononuclear cells in ≥ 50% of samples over 6 months. The results showed that 81 cases (52.94%) of recipients developed CHL after liver transplantation. CV-IPV (<i>OR</i> = 1.034, 95% <i>CI</i>: 1.006–1.063, <i>p</i> = 0.019) was an independent risk factor for CHL carriage. Additional risk factors included younger age, absence of mycophenolate mofetil use, and earlier timing of first EBV viremia. In conclusion, CHL carriage development in pediatric liver transplant recipients is positively correlated with Tac-IPV.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70562","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circulating Cell-Free Mitochondrial DNA as a Prognostic Biomarker in Patients With HBV-Related Acute-on-Chronic Liver Failure","authors":"Qiankun Hu,&nbsp;Jiajia Han,&nbsp;Chong Chen,&nbsp;Shuai Tao,&nbsp;Chenlu Huang,&nbsp;Jiacheng Lin,&nbsp;Xun Qi,&nbsp;Zhiping Qian,&nbsp;Mengxin Lu,&nbsp;Xinyan Li,&nbsp;Yi Zhang,&nbsp;Xuhua Jiang,&nbsp;Jianming Zheng,&nbsp;Huazhen Zhao,&nbsp;Feifei Yang,&nbsp;Jiming Zhang,&nbsp;Liang Chen,&nbsp;Xiaoni Kong,&nbsp;Xueyun Zhang,&nbsp;Yuxian Huang","doi":"10.1002/jmv.70551","DOIUrl":"https://doi.org/10.1002/jmv.70551","url":null,"abstract":"<div>\u0000 \u0000 <p>Acute-on-chronic liver failure (ACLF), particularly hepatitis B virus-related ACLF (HBV-ACLF), is a severe condition with high short-term mortality. This study aimed to evaluate the prognostic value of circulating cell-free mitochondrial DNA (cf-mtDNA) in predicting short-term mortality in HBV-ACLF patients. A total of 320 HBV-ACLF patients were included in the study population, with 192 patients in the derivation cohort and 128 in the validation cohort. Plasma cf-mtDNA levels were quantified using qPCR. Plasma cf-mtDNA levels were significantly elevated in HBV-ACLF patients (3.95 log₁₀ copies/μL) compared to healthy controls (2.99 log₁₀ copies/μL) and patients with chronic liver disease (3.03 log₁₀ copies/μL) (<i>p</i> &lt; 0.001). Plasma cf-mtDNA levels progressively increased with disease severity and were associated with multi-organ failure. Sequential measurements of cf-mtDNA showed a significant decrease in survivors (4.00 vs. 3.78 log₁₀ copies/μL, <i>p</i> = 0.019), while non-survivors maintained persistently elevated levels with minimal change (4.19 vs. 4.15 log₁₀ copies/μL, <i>p</i> = 0.359). The cut-off value of 3.9 log₁₀ copies/μL for cf-mtDNA effectively stratified patients into high- and low-risk groups, with significantly lower survival rates in the high-risk group (28-day: 46.2% vs. 86.0%, <i>p</i> &lt; 0.001; 90-day: 37.7% vs. 72.1%, <i>p</i> &lt; 0.001). Using cf-mtDNA in combination with key clinical parameters, we developed a novel prognostic score that exhibited superior predictive accuracy for 28- and 90-day mortality (AUROCs: 0.907 and 0.906, respectively), outperforming established prognostic scores (all <i>p</i> &lt; 0.05). These findings were validated in an independent cohort. Cf-mtDNA is a superior biomarker for predicting short-term mortality in HBV-ACLF. Its association with multi-organ failure and disease severity highlights the potential utility in early risk assessment and treatment optimization.</p></div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144861669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Variants Affect Distinct Metabolic Pathways in Pediatric Multisystem Inflammatory Syndrome and Severe COVID-19","authors":"Alysson Henrique Urbanski,&nbsp;Flávia Cristina de Paula Freitas,&nbsp;Tiago Minuzzi Freire da Fontoura Gomes,&nbsp;Michelle Orane Schemberger,&nbsp;Bárbara Carvalho Santos dos Reis,&nbsp;Flavia Amêndola Anísio de Carvalho,&nbsp;Roberta Soares Faccion,&nbsp;Lucas de Almeida Machado,&nbsp;Deborah Antunes dos Santos,&nbsp;Daniela Prado Cunha,&nbsp;Margarida dos Santos Salú,&nbsp;Daniella Campelo Batalha Cox Moore,&nbsp;Mayra Marinho Presibella,&nbsp;Juliana Fontes Noguchi,&nbsp;Henrique Lira Borges,&nbsp;Lais Kimie Tomiura,&nbsp;Luiza Silva de Castro,&nbsp;Letícia Graziela Costa Santos,&nbsp;Esdras Matheus Gomes da Silva,&nbsp;Vinícius Da Silva Coutinho Parreira,&nbsp;Luis Gustavo Morello,&nbsp;Fabricio Klerynton Marchini,&nbsp;Maria Regina Tizzot,&nbsp;Mauricio Marcondes Ribas,&nbsp;Gilberto Pascolat,&nbsp;Carmen Australia Paredes Marcondes Ribas,&nbsp;Fábio Fernandes da Rocha Vicente,&nbsp;Alexandre Rossi Paschoal,&nbsp;Rubens Cat,&nbsp;Benilton de Sá Carvalho,&nbsp;Jaqueline Carvalho de Oliveira,&nbsp;Marcus F. Oliveira,&nbsp;Luiz Lehmann Coutinho,&nbsp;Acácia Maria Lourenço Francisco Nasr,&nbsp;Irina Nastassja Riediger,&nbsp;Jeanine Marie Nardin,&nbsp;Liya Regina Mikami,&nbsp;Ana Carolina Ramos Guimarães,&nbsp;Patricia Savio de Araujo-Souza,&nbsp;Arnaldo Prata-Barbosa,&nbsp;Zilton Farias Meira de Vasconcelos,&nbsp;Helisson Faoro,&nbsp;Hellen Geremias dos Santos,&nbsp;Fabio Passetti","doi":"10.1002/jmv.70556","DOIUrl":"https://doi.org/10.1002/jmv.70556","url":null,"abstract":"<p>The coronavirus disease 2019 (COVID-19) pandemic has triggered a global health crisis, with over 700 million confirmed cases and at least 7 million deaths reported by early 2024. Children are less vulnerable to severe SARS-CoV-2 infection than adults and typically experience milder respiratory symptoms. However, a rare but significant complication, known as multisystem inflammatory syndrome in children (MIS-C), can develop weeks after infection, characterized by a spectrum of inflammatory symptoms. This study employed whole-exome sequencing and over-representation analysis to identify genetic variants of potential clinical significance related to MIS-C or severe COVID-19 in a group of children with acute respiratory distress syndrome (ARDS), all of whom were unvaccinated for COVID-19. We observed the enrichment of potentially pathogenic genetic variants in genes related to carbohydrate metabolism, particularly glycogen breakdown, in severe COVID-19 pediatric patients, and in genes related to cholesterol and lipoprotein metabolism in MIS-C patients. These findings offer insights into the genetic underpinnings of MIS-C and severe COVID-19, suggesting potential genes and biological pathways for further research.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70556","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144853856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Epidemiology of Coxsackievirus A10 Associated With Hand, Foot and Mouth Disease From 2021 to 2024 in Shenzhen, China","authors":"Long Chen,&nbsp;Xiang-Jie Yao,&nbsp;Hong Yang,&nbsp;Hai-Long Zhang,&nbsp;Xiao-Lu Shi,&nbsp;Bo Peng,&nbsp;Shi-Min Li,&nbsp;Jun Meng","doi":"10.1002/jmv.70552","DOIUrl":"https://doi.org/10.1002/jmv.70552","url":null,"abstract":"<div>\u0000 \u0000 <p>The study aimed to investigate epidemiological profile and molecular characteristics of coxsackievirus A10 (CVA10) associated with hand, foot and mouth disease (HFMD) in Shenzhen, China and comparatively analyze genomes of CVA10 strains related to differential clinical phenotypes. A total of 3170 clinical specimens collected between 2021 and 2024 were examined for CVA10 using real-time RT-PCR. Complete VP1 sequences and near-complete genome sequences of CVA10 were determined by RT-PCR methods and sequencing. Sequences were analyzed using a series of bioinformatics programs. Two (33.33%) out of 6 severe cases were infected with CVA10. The detection rate of CVA10 associated with mild HFMD ranged from 1.21% to 6.11% in 2021–2024, with an overall detection rate of 3.73%. There was no significant difference in the infection rate of CVA10 between males and females or different age groups. The CVA10 infections mainly occurred in Spring (March to May) and Summer (June to August) in Shenzhen. Of the 74 VP1 sequences determined, 71 (95.95%) of them were detected in the sub-genotype C2, 3 (4.05%) were assigned to the genotype D. Genomic sequence analysis indicated that the genotype D of CVA10 of this study derived from genetic recombination between CVA10 and CVA16 in 3A–3D coding region (nucleotide position: 5075-6896). Different variable sites were observed in the two CVA10 strains associated with different severe complications when compared to CVA10 strains associated with mild diseases. In conclusion, CVA10 associated with HFMD circulated at a low level in Shenzhen in 2021–2024, with C2 as the predominant genotype. Recombinant genotype D of CVA10 was introduced first to Shenzhen in 2024. The study emphasizes the importance of continuous molecular surveillance of CVA10.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144853860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"eIF2α Kinases Involve in the Regulation of VSV Replication Through CHOP in SMMC7721 Cells","authors":"Runxin Luan,&nbsp;Yiyuan Shang,&nbsp;Mingjie Chen,&nbsp;Senyue Li,&nbsp;Rong Zhang,&nbsp;Shengzhen Sun,&nbsp;Panlin Wang,&nbsp;Dan Song,&nbsp;Yanan Zhang,&nbsp;Xiangchen Li,&nbsp;Yongshu Wu","doi":"10.1002/jmv.70548","DOIUrl":"https://doi.org/10.1002/jmv.70548","url":null,"abstract":"<div>\u0000 \u0000 <p>Previous studies have shown that eIF2α kinases involved in viral replication through eIF2α phosphorylation upon vesicular stomatitis virus (VSV) infection. The oncotherapy approach of VSV is based on its inducing controlled apoptosis in tumor cells. In this study, we explorated the role of eIF2α kinases in VSV replication and CHOP expression in SMMC7721 cell lines. We found that three eIF2α kinases involved in VSV replication, PERK inhibited viral replication through eIF2α phosphorylation, both PKR and GCN2 initiated CHOP expression to favor viral replication at late stage, and HRI had no effect on VSV replication. These findings confirmed that eIF2α kinases initiate an integrated response and regulate viral replication to restore cellular homeostasis upon viral infection. The activation of CHOP expression may facilitate the release and spread of viral progeny, enrich the characteristics of VSV-induced apoptosis through CHOP expression, and provide a strategy for cancer therapy.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the Global Burden of Rotavirus-Induced Diarrheal Diseases: Trends From 1990 to 2021 and Projections for 2050","authors":"Dingjun Wang,&nbsp;Xiaotong Lai,&nbsp;Xinghan Ren,&nbsp;Xinyue Shen,&nbsp;Hongbo Jiang,&nbsp;Tiantian Liu","doi":"10.1002/jmv.70559","DOIUrl":"https://doi.org/10.1002/jmv.70559","url":null,"abstract":"<div>\u0000 \u0000 <p>This study utilizes Global Burden of Diseases (GBD) 2021 data to analyze trends and predictions related to rotavirus-induced diarrheal diseases. We extracted data from GBD 2021 to assess overall and regional trends in rotavirus-induced diarrheal diseases. We calculated relative risks associated with age, period, and birth cohort, and fitted curves relating disease burden to the Sociodemographic Index (SDI) for various countries, with predictions extending to 2050. In 2021, ~170 000 deaths globally were attributed to rotavirus-induced diarrheal diseases. While the global burden has significantly declined since 1990, populations in low SDI regions remain heavily affected, and an upward trend is observed in High SDI regions. Newborns aged 0–6 days are particularly vulnerable, with the disability-adjusted life years (DALY) rate decreasing as age increases. For those aged 45 and older, the burden rises steadily, peaking at ages 80–90. Predictions suggest a continued decline by 2050. Despite progress in rotavirus prevention, economic challenges in High SDI regions threaten healthcare funding and increase transmission risks. Ongoing global economic downturns and conflicts may hinder efforts, underscoring the need for informed policy formulation to address these challenges effectively.</p></div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-Country Inequalities in Hepatitis E Virus Infection From 1990 to 2021: A Brief Analytical Insights From the Global Burden of Disease Study 2021","authors":"Mengting Liu,&nbsp;Xiaomeng Zhang,&nbsp;Zhongyan Xu","doi":"10.1002/jmv.70560","DOIUrl":"https://doi.org/10.1002/jmv.70560","url":null,"abstract":"","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance of Genotype-Specific High-Risk Human Papillomavirus Testing in Cervical Cancer Screening: A Hospital-Based Study","authors":"Sarocha Boonkate,&nbsp;Wathirada Karnchanabanyong,&nbsp;Irene Ruengkhachorn,&nbsp;Sompop Kuljarusnont,&nbsp;Nida Jareemit,&nbsp;Navin Horthongkham,&nbsp;Archiraya Pattama,&nbsp;Sukanya Athipanyasilp,&nbsp;Suchanan Hanamornroongruang","doi":"10.1002/jmv.70561","DOIUrl":"https://doi.org/10.1002/jmv.70561","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>This study explored histopathological outcomes among women who tested positive for high-risk human papillomavirus (hrHPV), examined the significance of extended HPV genotyping, and identified predictors of cervical intraepithelial neoplasia grade 2 or worse (CIN2⁺). This retrospective review assessed medical records of women who screened positive for hrHPV between January 1, 2020, and December 31, 2023. Genotyping results, diagnostic procedures, and histopathological findings were collected. Data were analyzed using SPSS, with <i>p</i> &lt;  0.05 considered statistically significant. Among 1981 women, the median age was 40 years (IQR 32.0‒49.0), and the median parity was 1 (IQR 0‒2). Overall, 1223 women (61.7%) had prior screening, 1215 women (61.3%) had previous cytology, and 107 women (5.4%) had prior hrHPV testing. Single-genotype infection occurred in 1408 women (74.7%), with HPV52, HPV16, and HPV58 identified in 23.7%, 15.6%, and 15.4% of cases, respectively. CIN2⁺ was detected in 152 women (7.7%), including 130 with CIN2/CIN3/AIS and 22 with cancer. Detection of HPV16 significantly increased the risk of CIN2⁺ (odds ratio [OR] 4.534, 95% CI: 3.197‒6.430), as did multiparity (OR 1.497, 95% CI: 1.070‒2.094). The immediate risk of CIN2⁺ for HPV31, HPV39, HPV56, HPV66, and HPV68 was below 4%. Among hrHPV-positive women, 7.7% had CIN2⁺. Extended hrHPV genotyping may refine risk stratification by highlighting HPV16 and multiparity as significant predictors of CIN2⁺ lesions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70561","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurological Manifestations in Oropouche Virus Infection: A Systematic Review and Meta-Analysis","authors":"Ranjit Sah,&nbsp;Prakasini Satapathy,&nbsp;Abhay M. Gaidhane,&nbsp;Nasir Vadia,&nbsp;Soumya V. Menon,&nbsp;Kattela Chennakesavulu,&nbsp;Rajashree Panigrahi,&nbsp;Ganesh Bushi,&nbsp;Mahendra Singh,&nbsp;Sanjit Sah,&nbsp;Rachana Mehta,&nbsp;Awakash Turkar,&nbsp;S. Govinda Rao,&nbsp;Khang Wen Goh,&nbsp;Muhammed Shabil,&nbsp;Andrea G. Rodriguez-Morales,&nbsp;Tania do Socorro Souza Chaves,&nbsp;Pasesa Quispe-Torrez,&nbsp;Rodrigo Nogueira Angerami,&nbsp;Bijaya Kumar Padhi,&nbsp;Alfonso J. Rodriguez-Morales","doi":"10.1002/jmv.70532","DOIUrl":"https://doi.org/10.1002/jmv.70532","url":null,"abstract":"<div>\u0000 \u0000 <p>Oropouche virus (OROV), an emerging arbovirus, poses a significant public health concern in tropical and subtropical regions of Latin America, as well as in other parts of the world, with imported cases reported in North America and Europe. While OROV is primarily associated with acute febrile illness, especially emerging evidence suggests it may cause neurological complications, though these remain understudied. This systematic review and meta-analysis aim to estimate the prevalence of neurological manifestations in OROV infections. Following the PRISMA 2020 guidelines, a systematic literature search was conducted across PubMed, Web of Science, and Embase up to January 25, 2025, and registered in PROSPERO (Registration ID: CRD42025634617). Nested Knowledge software was employed for the screening and data extraction processes. Data extraction and quality assessment were performed using a modified version of the Newcastle-Ottawa Scale. A meta-analysis was conducted using R software to estimate the pooled prevalence rates of neurological manifestations, with heterogeneity assessed using the I² statistic. Sensitivity analyses and publication bias assessments were also performed. Ten studies from Brazil, Peru, and Colombia were included, encompassing a total of 2872 patients. The pooled prevalence of neurological symptoms was high, with headache (89.16%), myalgia (70.71%), and eye pain (52.87%) being the most common. Other symptoms included arthralgia (56.5%), back pain (46.1%), and nausea (43.3%). Significant heterogeneity was observed across studies, likely due to variations in geography and diagnostic methods. Sensitivity analyses confirmed the robustness of the findings. Neurological manifestations are prevalent in OROV infections, with headache, myalgia, and eye pain being the most frequent. The clinical overlap with other arboviruses complicates diagnosis, underscoring the need for improved diagnostic tools and surveillance of neurological syndromes associated with arboviruses in endemic regions and newly emerging areas with recent circulation of the virus. To improve generalizability, future research should broaden geographic analyses and concentrate on longitudinal and standardized studies to better understand the temporal dynamics of symptoms.</p></div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 8","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}