Journal of Medical Virology最新文献

{"title":"Correction to “Effects of Different Feeding Patterns on the Gut Virome of 6-Month-Old Infants”","authors":"","doi":"10.1002/jmv.70623","DOIUrl":"https://doi.org/10.1002/jmv.70623","url":null,"abstract":"<p>Pan, C., Xu, P., Yuan, M., Wei, S., Lu, Y., Lu, H. and Zhang, W. (2025). Effects of Different Feeding Patterns on the Gut Virome of 6-Month-Old Infants. Journal of Medical Virology, 97: e70344. https://doi.org/10.1002/jmv.70344</p><p>In the published article, on page 4, the captions for “Figure 2”, “Figure 3”, and “Figure 4” were misplaced. Specifically, the text currently labeled for one figure corresponds to another figure, failing to establish the correct correspondence between the figures and their captions. The correct correspondence should be as follows:</p><p>Figure 2. The composition of enterovirus family. (A). Heatmap representing the reads number of each viral family of individual library on log10 scale. The composition of the virus genome is represented by rectangles of different colors, and the name of the virus family is represented on the right. Sample groups and virus family types are displayed in the corresponding colors (see color legend). (B). The bar chart shows the proportion of different virus families in each individual library.</p><p>Figure 3. Comparison of virus alpha diversity (Shannon index) between groups of different feeding patterns at the (A) order and (B) family. The horizontal bars inside the box represent the median values.</p><p>Figure 4. PCoA analysis of different feeding patterns at the level of the order (A) and the family (B).</p><p>We apologize for this error.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 10","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jmv.70623","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145146679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Specific Sexually Transmitted Pathogens on Cervix: A Prospective Study Based on Cervical Cancer Screening Cohort","authors":"Simiao Chen,&nbsp;Tingyuan Li,&nbsp;Yakun Wang,&nbsp;Yu Dai,&nbsp;Qinjing Pan,&nbsp;Zhihui Zhang,&nbsp;Xun Zhang,&nbsp;Qiong Liao,&nbsp;Shijun Jia,&nbsp;Dongsheng Wang,&nbsp;Lingling Zhu,&nbsp;Xingsheng Cai,&nbsp;Chunlin Wang,&nbsp;Lingmei Yan,&nbsp;Xiaoyan Le,&nbsp;Hua Yang,&nbsp;Youlin Qiao,&nbsp;Jennifer S. Smith,&nbsp;Yuqian Zhao,&nbsp;Lan Zhu,&nbsp;Wen Chen","doi":"10.1002/jmv.70616","DOIUrl":"https://doi.org/10.1002/jmv.70616","url":null,"abstract":"<div>\u0000 \u0000 <p>Previous studies showed the association between sexually transmitted infections (STIs) and cervical lesions remains ambiguous. This study was conducted among 8371 women from a screening cohort. Seven specific sexually transmitted pathogens (STPs), including one viral [high-risk human papillomavirus (hrHPV), low-risk HPV (lrHPV)], five bacterial [Ureaplasma parvum (UP), Mycoplasma hominis (MH), Ureaplasma urealyticum (UU), Chlamydia trachomatis (CT), and Mycoplasma genitalium (MG)], and one parasitic [Trichomonas vaginalis (TV)] pathogen, were tested by Next Generation Sequencing assay using well-stored baseline samples. Odds ratios (ORs) for incident cervical lesions with different STPs were calculated by Logistic Regression analysis. Within 3-year follow-up, 133 and 72 participants were diagnosed with histopathological cervical intraepithelial neoplasia grade 1 (CIN1) and CIN2+, respectively. The adjusted ORs (aORs) of atypical squamous cells of undetermined significance or worse (ASC-US+) for women with hrHPV, lrHPV, UP, MH, TV, CT, and MG infections were 2.62 (95% CI: 2.19–3.13), 1.94 (95% CI: 1.55–2.43), 1.48 (95% CI: 1.26–1.74), 1.47 (95% CI: 1.25–1.73), 1.65 (95% CI: 1.27–2.15), 1.26 (95% CI: 0.79–2.01) and 2.33 (95% CI: 1.41–3.85), respectively. The aORs of cytological high-grade squamous intraepithelial lesions (HSIL) for women with hrHPV, TV, and MG infections were 13.01 (95% CI: 5.78–29.31), 3.48 (95% CI: 1.38–8.75), and 5.87 (95% CI: 1.58–21.77). The aORs of CIN1 for hrHPV, lrHPV, and MH were 6.88(95% CI: 4.79–9.90), 2.04(95% CI: 1.29–3.14), and 1.47(95% CI: 1.02–2.11). The aOR of CIN2+ for women with hrHPV infection was 17.56 (95% CI: 10.31–29.92), no significance was observed for CIN2+ with non-hrHPV STIs. Specific STP infections were significantly associated with subsequent cervical cytological ASC-US+ (hrHPV, lrHPV, UP, MH, TV, and MG) and HSIL (hrHPV, TV, and MG). Infection with lrHPV and MH could increase the CIN1 risk in future though no obvious CIN2+ risk elevation was observed.</p></div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 10","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145146680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemagglutinin of Emerging Low Pathogenic Avian Influenza Viruses Underpins High Pathogenicity to Mammals","authors":"Xiaoyi Gao,&nbsp;Jinze Dong,&nbsp;Linlin Wang,&nbsp;Chuankuo Zhao,&nbsp;Xinsen Li,&nbsp;Shujun Zhang,&nbsp;Yudong Li,&nbsp;Yong Zhou,&nbsp;Wenjing Peng,&nbsp;Yanxin Hu,&nbsp;Qi Tong,&nbsp;Litao Liu,&nbsp;Honglei Sun,&nbsp;Yipeng Sun,&nbsp;Jinhua Liu,&nbsp;Zhimin Jiang,&nbsp;Juan Pu","doi":"10.1002/jmv.70615","DOIUrl":"https://doi.org/10.1002/jmv.70615","url":null,"abstract":"<div>\u0000 \u0000 <p>The pathogenicity of emerging early zoonotic avian H7N9 and H3N8 low-pathogenic avian influenza viruses (LPAIVs) in humans is significantly enhanced compared to that of their internal gene providers, H9N2 avian influenza viruses (AIVs), suggesting a pivotal role for surface HA or NA. Here, we generated several reassortant AIVs that combined HA, NA, or HA + NA from emerging zoonotic H7N9 or H3N8 LPAIV with internal H9N2 AIV genes and investigated the impact of HA and NA on the replication and pathogenicity of reassortants in human cells and mice. The crucial affected phase was determined by analyzing the receptor binding, viral adsorption, HA cleavage efficiency, viral endocytosis, and budding. We found that mice infected with the virus containing the early zoonotic H7N9 LPAIV HA, but not NA, exhibited high mortality, weight loss, severe lung damage, and increased viral load in the lungs. We found that HA substitution enhanced viral replication in human A549 cells and displayed dual sialic acid receptor binding ability. This substitution also facilitated viral attachment to mammalian cells and promoted endocytosis by enhancing HA0 cleavage efficiency; however budding was not affected. Additionally, HA from emerging zoonotic H3N8 LPAIVs elevate the pathogenicity of reassortants in mice. Together, our study revealed that HA of emerging zoonotic LPAIVs contributes to high pathogenicity in mammals by augmenting viral entry and causing lung injury, thereby highlighting HA with double receptor binding properties and HA cleavage efficiency as new markers for risk assessment of emerging zoonotic AIVs in the future.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 10","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145146769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determinants of Changes in HPV Vaccination Willingness Among Men Who Have Sex With Men: An Observational Cohort Study","authors":"Jingjing He,&nbsp;Tian Tian,&nbsp;Xiaoqing Tuo,&nbsp;Wenhui Yu,&nbsp;Shulipan Asilibieke,&nbsp;Zewen Zhang,&nbsp;Lirong Liu,&nbsp;Miaomiao Xi,&nbsp;Heng Yang,&nbsp;Guozhen Zhang,&nbsp;Jianghong Dai","doi":"10.1002/jmv.70611","DOIUrl":"10.1002/jmv.70611","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>Human papillomavirus (HPV) infection is highly prevalent among men who have sex with men (MSM) and increases the risk of cancer; however, vaccine uptake remains low. Our objective was to explore the factors influencing changes in HPV vaccination willingness among MSM. HIV-negative MSM aged ≥ 18 years in Xinjiang, China, were enrolled in an observational cohort study conducted from March 1, 2017, to March 30, 2023. A self-administered questionnaire was used to collect baseline and follow-up data every 6 months on HPV vaccination willingness, demographics, sexual behaviors, and HPV knowledge. Anal swabs were used for HPV genotyping. Participants were categorized based on their longitudinal willingness as consistently willing, transitioning, or consistently unwilling. Multivariable logistic regression was used to analyze determinants of changes in vaccination willingness. Among 746 enrolled MSM (median age 30 [IQR:25–36]), 442 (59.2%) were consistently willing to vaccinate, 160 (21.4%) transitioned to willingness, and 144 (19.4%) were consistently unwilling. In multivariate analysis, lower education (high school or below: adjusted odds ratio (AOR) = 2.4, 95% confidence interval (95% CI): 1.4–4.1; some college: AOR = 1.8, 95% CI: 1.1–2.9), lower income (1001–5000 yuan: AOR = 0.39, 95% CI: 0.19–0.77; 5001–10 000 yuan: AOR = 0.26, 95% CI: 0.13–0.54; ≥ 10 001 yuan: AOR = 0.14, 95% CI: 0.05–0.42), and lower HPV knowledge (AOR = 0.6, 95% CI: 0.4–0.9) were independently associated with vaccination willingness. HPV vaccine willingness was low but dynamic among MSM. Addressing modifiable factors, such as HPV knowledge, may improve uptake.</p>\u0000 </section>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 10","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Mini-Hemagglutinin Subunit Vaccine Induces Robust Mucosal and Systemic Immunity and Protection Against a Lethal Challenge With the H1N1 and H5N1 Influenza Viruses in Mice","authors":"Tangqi Wang,&nbsp;Ruiwen Han,&nbsp;Jia Li,&nbsp;Xueting Cheng,&nbsp;Zhenyong Qi,&nbsp;Jialuo Bing,&nbsp;Chengcheng Zhai,&nbsp;Donghong Wang,&nbsp;Yao Deng,&nbsp;Wenjie Tan","doi":"10.1002/jmv.70614","DOIUrl":"10.1002/jmv.70614","url":null,"abstract":"<div>\u0000 \u0000 <p>Conventional influenza vaccines target the globular head domains of hemagglutinins (HAs), which are highly plastic and provide strain-specific immunity. Therefore, a universal influenza vaccine with conserved antigens and novel adjuvants is urgently required. Mucosal immunity is the first line of defense against viral infections; however, proven mucosal adjuvants are lacking. Therefore, we developed an intranasal Mini-HA subunit vaccine combined with the CTA1-DD adjuvant, which elicited robust mucosal and systemic immune responses in the lungs and spleens of mice, including antigen-specific IgA, IgG, and effector T cells. Furthermore, the Mini-HA subunit vaccine provided the mice with cross-protection against H1N1 and H5N1 influenza viruses. These results indicate that combining the Mini-HA antigen and mucosal adjuvant CTA1-DD is a promising strategy for developing broad-spectrum influenza vaccines.</p></div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 10","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytomegalovirus UL21-5 mRNAemia as a Marker of Active Virus Replication in Allogeneic Hematopoietic Transplant Recipients","authors":"Carlos Solano de la Asunción,&nbsp;María Dolores Ocete,&nbsp;Estela Giménez,&nbsp;Eliseo Albert,&nbsp;Concepción Gimeno,&nbsp;José Luis Piñana,&nbsp;Carlos Solano,&nbsp;David Navarro","doi":"10.1002/jmv.70617","DOIUrl":"10.1002/jmv.70617","url":null,"abstract":"","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 10","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection and Phylogenetic Characterization of Human Aichi Virus in Sewage by Next Generation Sequencing in Eastern China, 2019–2022","authors":"Meng Chen,&nbsp;Yao Liu,&nbsp;Xiaojuan Lin,&nbsp;Feng Ji,&nbsp;Suting Wang,&nbsp;Aiqiang Xu,&nbsp;Zexin Tao","doi":"10.1002/jmv.70603","DOIUrl":"10.1002/jmv.70603","url":null,"abstract":"<div>\u0000 \u0000 <p>Aichi virus (AiV) infection is associated with gastroenteritis symptoms, and sewage surveillance has emerged as an effective approach for assessing AiV prevalence. However, a comprehensive understanding of its epidemiology and genetic diversity remains limited, particularly in China. In this study, monthly sewage samples were collected from Jinan, China, between 2019 and 2022. The AiV genome was quantitatively analyzed, and the VP1 region was amplified from sewage concentrates for subsequent next-generation sequencing (NGS). The results revealed a remarkably high detection rate of 95.83% (46/48) over the 4-year study period. Quantitative analysis showed AiV genome concentrations ranging from 1.4 × 10³ to 2.7 × 10⁵ genomic copies per liter in sewage, with distinct seasonal patterns and peak occurrences during summer and autumn. Phylogenetic analysis of the VP1 region revealed that Jinan sequences clustered into three groups within genotypes A and B, with an average <i>p</i>-distance of 0.091. Genotype A was detected in all sewage samples (100.00%), while genotype B was found in 52.50% of samples. In terms of relative abundance, they constituted 86.06% and 13.94% of the total clean reads, respectively. This study represents the first application of amplicon NGS for AiV detection in sewage samples in China, revealing the continuous co-circulation of multiple transmission lineages and significant genetic diversity of AiV in wastewater systems. The findings demonstrate that NGS technology significantly enhances detection sensitivity and provides substantially more comprehensive data for the molecular epidemiology of AiV.</p>\u0000 </div>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 9","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Pyrido-Quinoxaline Derivative That Downregulates Reticulon 3 Protein Exhibits Potent Antiviral Activity Against Zika Virus","authors":"Erika Plicanti,&nbsp;Andrea Deiana,&nbsp;Silvia Nottoli,&nbsp;Giulia Lottini,&nbsp;Roberta Ibba,&nbsp;Sandra Piras,&nbsp;Carlo Di Marzo,&nbsp;Silvia Vegni,&nbsp;Michele Lai,&nbsp;Mauro Pistello,&nbsp;Antonio Carta,&nbsp;Giulia Freer","doi":"10.1002/jmv.70605","DOIUrl":"10.1002/jmv.70605","url":null,"abstract":"<p>In the wake of the COVID-19 pandemic, awareness of emerging pathogens has significantly increased, prompting greater investment in research and preparedness. In this context, arboviral diseases are recognized as unmet medical challenges due to their rapid spread. Notably, the geographical range of several flaviviral diseases is expanding: Zika virus (ZIKV), a member of the <i>Flaviviridae</i> family, has recently been linked to outbreaks associated with a rise in microcephaly cases in tropical regions. To contribute to the development of novel antiviral therapies, evaluation of a set of compounds with an antiviral activity against ZIKV was carried out. These compounds were originally identified as inhibitors of bovine viral diarrhea virus, another member of the <i>Flaviviridae</i> family. Two related compounds turned out to be active against ZIKV. One emerged as a particularly strong antiviral candidate, demonstrating high efficacy in inhibiting ZIKV replication, and became the focus of this study. Its activity was tested against a number of viruses of human health relevance and the compound was found to be effective against a number of viruses that use the endoplasmic reticulum as a replication hub. Indeed, we found that the Reticulon 3 protein is potently downregulated in the presence of the compound, whereas other endoplasmic reticulum-resident proteins are not affected. Because Reticulon 3 has a role in the replication of positive-sense single-stranded RNA viruses, an indirect antiviral effect of the compound studied was hypothesized. This compound may be considered as a promising lead for further studies aimed at the development of broad-spectrum antiviral drugs.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 9","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12445440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virus–Host Interaction: Investigating Novel Transcription Factors Involved in Coupling Human Papillomavirus Life Cycle and Epithelial Differentiation","authors":"Aline L. Ribeiro,&nbsp;Valéria Talpe-Nunes,&nbsp;Amanda S. Caodaglio,&nbsp;Rafaella A. L. Nunes,&nbsp;João S. P. Sobrinho,&nbsp;Laura Sichero","doi":"10.1002/jmv.70610","DOIUrl":"10.1002/jmv.70610","url":null,"abstract":"<p>High-risk human papillomavirus (HPV) is the etiological agent of nearly all cervical cancers, with HPV-18 being the second most prevalent type. The HPV life cycle is closely associated with epithelial differentiation, a process governed by cellular transcription factors (TFs). During progression toward malignancy, HPV disrupts differentiation and promotes uncontrolled cell proliferation. We aimed to identify differentiation-modulated TFs linking viral transcription to differentiation, providing insights into mechanisms driving viral life cycle and pathogenesis. DNA-binding activity of 345 TFs was compared between undifferentiated and differentiated keratinocytes to identify differentiation-associated TFs. In silico analyses identified putative binding sites for these TFs within the HPV-18 long control region (LCR). Chromatin immunoprecipitation confirmed direct binding of PAX6, HMGB1, and NFE2 to the LCR, but not FOXI1. Immunohistochemistry in keratinocyte raft cultures demonstrated differentiation-dependent expression patterns for all four TFs. Functional assays revealed that each TF is capable of modulate HPV-18 early promoter activity, with effects varying by differentiation status. Notably, expression of these TFs was disrupted by the viral oncoproteins E6 and E7, underscoring a mechanism by which HPV alters host differentiation. These findings identify novel differentiation-linked regulators of HPV-18 transcription and highlight host targets exploited by the virus in its life cycle and pathogenesis.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 9","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Japanese Encephalitis Virus Genotype 5 Infectious Clone and Reporter System for Antiviral Evaluation","authors":"Jae-Yeon Park,&nbsp;Wataru Kamitani,&nbsp;Hyun-Jin Shin,&nbsp;Hye-Mi Lee","doi":"10.1002/jmv.70608","DOIUrl":"10.1002/jmv.70608","url":null,"abstract":"<p>The recent emergence of Japanese encephalitis virus genotype 5 (JEV5) has raised concerns regarding limited vaccine efficacy. Here, we report the construction of a full-length infectious cDNA clone of JEV5 using a bacterial artificial chromosome (BAC) system, based on a South Korea isolate, K15P38 strain. The resulting clone enabled recovery of replication-competent virus and was genetically distinguishable from the parental strain via engineered silent mutations. To extend its application, we introduced nanoluciferase and enhanced green fluorescent protein into the viral genome, generating reporter viruses suitable for replication tracking. Both reporter viruses supported real-time quantification of viral replication and demonstrated utility in antiviral compound screening. Antiviral evaluation using the nucleoside analog NITD008 yielded EC₅₀ values consistent between reporter and classical plaque assays. These findings provide a reverse genetics platform for studying JEV5 and suggest applicability for genotype-specific vaccine and antiviral research.</p>","PeriodicalId":16354,"journal":{"name":"Journal of Medical Virology","volume":"97 9","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12439625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}