{"title":"Toxicity of Glyphosate on Physalaemus albonotatus (Steindachner, 1864) from Western Brazil","authors":"F. Simioni, Débora Guimarães da Silva, T. Mott","doi":"10.5132/EEC.2013.01.008","DOIUrl":"https://doi.org/10.5132/EEC.2013.01.008","url":null,"abstract":"Amphibian declines have been reported worldwide and pesticides can negatively impact this taxonomic group. Brazil is the world’s largest consumer of pesticides, and Mato Grosso is the leader in pesticide consumption among Brazilian states. However, the effects of these chemicals on the biota are still poorly explored. The main goals of this study were to determine the acute toxicity (CL50) of the herbicide glyphosate on Physalaemus albonotatus, and to assess survivorship rates when tadpoles are kept under sub-lethal concentrations. Three egg masses of P. albonotatus were collected in Cuiaba, Mato Grosso, Brazil. Tadpoles were exposed for 96 h to varying concentrations of glyphosate to determine the CL50 and survivorship. The CL50 was 5.38 mg L-1 and there were statistically significant differences in mortality rates and the number of days that P. albonotatus tadpoles survived when exposed in different sub-lethal concentrations of glyphosate. Different sensibilities among amphibian species may be related with their historical contact with pesticides and/or specific tolerances. Further studies are required to determine the degree to which this taxonomic group is threatened by chemical contaminants.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125512036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Patricia M. Castañé, B. L. Eissa, Natalia Alejandra Ossana
{"title":"Respuesta de biomarcadores bioquímicos, morfológicos y comportamentales de la carpa común, Cyprinus carpio, por exposición a muestras ambientales","authors":"Patricia M. Castañé, B. L. Eissa, Natalia Alejandra Ossana","doi":"10.5132/EEC.2013.01.006","DOIUrl":"https://doi.org/10.5132/EEC.2013.01.006","url":null,"abstract":"Se caracterizo la calidad ecotoxicologica del agua del rio Lujan, en 3 sitios de su trayecto con grado dispar de estres, mediante bioensayos semi-estaticos utilizando la carpa comun, Cyprinus carpio. Con un dispositivo y software especial se registro diariamente la ubicacion espacial de los peces durante el periodo control (agua potable) y el de exposicion (agua del rio), y se calculo el Indice de Actividad Relativa (Ia). Se calculo el Factor de Condicion (FC), y en cerebro se midio la actividad acetilcolinesterasica (AChE) y el contenido de proteinas totales. La morfologia de las branquias se analizo por microscopia electronica de barrido (MEB). En las muestras de agua se midieron: conductividad, pH, dureza, oxigeno disuelto, nitratos, cloruros, solidos totales y metales pesados. Se observo en los peces expuestos al agua del rio: a) un incremento significativo del Ia, y de AChE, b) la MEB revelo hipertrofia celular y ligera fusion de las lamelas secundarias. En algunos casos los metales excedieron los limites maximos para proteccion de la vida acuatica. En todas las muestras del rio se observo una mezcla compleja de metales pesados y algunos cationes y aniones, que produjeron efectos subletales en los organismos, puestos de manifiesto por la respuesta de los biomarcadores determinados.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131531903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multixenobiotic resistance mechanism monitoring: standardization of fluorescence emmited by Rhodamine B","authors":"M. L. Pessatti, J. D. Fontana","doi":"10.5132/EEC.2013.01.014","DOIUrl":"https://doi.org/10.5132/EEC.2013.01.014","url":null,"abstract":"Many aquatic organisms express the multixenobiotic resistance mechanism (MXR) mediated by a membrane protein denominated P-glycoprotein (Pgp), which reduce the accumulation of xenobiotics by active transport to out of cells. In order to establish fluorescence microscopy as a quantitative method to monitor MXR activity, the kinetic of fluorescence decay emitted by rhodamine B (RB) was determined. Rhodamine B (1 at 1000 nmoles L-1) were spotted on silica gel plate and fluorescence decay recorded and determined as exposition time (ET, sec) using a fluorescence microscope’s photo sensor. The ET at zero time was obtained from a linear equation of plotting ET(s) against the respective rhodamine B concentrations. The resulting mathematical model (RB = (28/ET) -1 -Blank, r2 = 0.9945), allowed the quantitative determination of rhodamine B intracellular accumulation (nmoles L-1This transport activity quantitation is consequence of the MXR mechanism activity operating in viable sections of gills of the mussel Perna perna and allows its measurement as a molecular biomarker.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"83 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132476492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. J. S. Filho, Vanice Koschier da Fonseca, Luciane Holbig
{"title":"Avaliação de metais em pescado da região do Pontal da Barra, Laguna dos Patos, Pelotas-RS","authors":"P. J. S. Filho, Vanice Koschier da Fonseca, Luciane Holbig","doi":"10.5132/EEC.2013.01.015","DOIUrl":"https://doi.org/10.5132/EEC.2013.01.015","url":null,"abstract":"Os metais pesados tem a capacidade de acumular-se em tecidos vivos ao longo da cadeia alimentar. Peixes podem se tornar a principal forma de transferir para a populacao esses elementos, uma vez que eles sao capazes de bioacumulacao e bioconcentracao. Com o objetivo de investigar a bioacumulacao nos peixes no Pontal da Barra-Laguna dos Patos, em Pelotas, foram escolhidas tres especies abundantes na regiao, Micropogonias furnieri (Corvina), Mugil platanus (Tainha) e Netuma barba (Bagre). Amostras da sua musculatura foram analisados para verificar o conteudo de Al, Cd, Cr, Cu, Fe, Mn, Ni, Pb e Zn. Amostras em quintuplicata sofreram digestao acida HNO3/HCIO4, sendo os extratos analisados por plasma indutivamente acoplado - espectrometria de emissao optica (ICP-OES). Os niveis de cromo encontrados nas tres especies estavam acima dos estabelecidos pela legislacao brasileira (Decreto-Lei 55,871-65), que determinam como um nivel maximo tolerado para o cromo 0,1 mg kg-1. Os 82,46 ± 4,69% mg kg-1 de zinco na especie, Netuma barba, tambem excedeu o limite permitido. As concentracoes de cadmio encontrada na especie Micropogonias furnieri, 0,92 ± 8,16% mg kg-1 ficaram proximas ao limite permitido. Os demais analitos, encontraram-se em niveis inferiores aos estabelecidos pelo Ministerio da Saude e Anvisa.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114782613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. B. Jesus, J. Colombi, C. Ribeiro, H. Assis, C. Carvalho
{"title":"Cholinestarase activity in methylmercury and mercury chloride exposure fish","authors":"T. B. Jesus, J. Colombi, C. Ribeiro, H. Assis, C. Carvalho","doi":"10.5132/EEC.2013.01.022","DOIUrl":"https://doi.org/10.5132/EEC.2013.01.022","url":null,"abstract":"In the current study, an intraperitoneal injection containing the same concentration of MeHg and HgCl2 was administered in Hoplias malabaricus, after 96 hours fish were anesthetized and the muscle tissue were removed. A significant inhibition of the ChE was observed the fish exposed to MeHg, compared to control. The present work demonstrates that ChE is an effective biomarker in fish exposed via intraperitoneal injection to the organic forms of mercury.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"95 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124051182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Artal, G. Almeida, A. Caloto-Oliveira, Maria Alice Santos, G. Umbuzeiro, Cassiana Maria Reganhan Coneglian
{"title":"Toxicity assessment of an effluent derived from an inactivated uranium mine: the Poços de Caldas (Brazil) example","authors":"M. Artal, G. Almeida, A. Caloto-Oliveira, Maria Alice Santos, G. Umbuzeiro, Cassiana Maria Reganhan Coneglian","doi":"10.5132/EEC.2013.01.005","DOIUrl":"https://doi.org/10.5132/EEC.2013.01.005","url":null,"abstract":"Uranium mines can cause environmental impact because of acid mine drainages can reaches the surrounding water bodies. In this work we tested effluent samples from an inactivated uranium mine located in Pocos de Caldas city (Minas Gerais, Brazil) for acute and chronic toxicity. Untreated effluents were acutely toxic to Daphnia similis, with EC50 (48 h) varying from <0.01% to 38%. Both treated and untreated effluent samples presented chronic toxicity to Ceriodaphnia dubia. Vibrio fischeri acute toxicity tests were negative for all tested samples. Adjustment of pH performed in samples was not sufficient to remove acute toxicity indicating that not only pH was responsible for the observed effect. The physical-chemical treatment applied was not able to remove the chronic toxicity to C. dubia. V. fischeri, although a rapid test and easy to handle, was not sensitive to the level of toxicants present in the effluent from the mine. To determine the actual impact of the discharge of effluent into receiving waters, both the flow of the effluent and river should be considered. Nevertheless chronic toxicity tests with C. dubia could be included in the monitoring program of this facility to provide more information about the impact of this discharge.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117328703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects on DNA and cell viability of treated water contaminated with Cylindrospermopsis raciborskii extract including cylindrospermopsin","authors":"A. Fonseca, A. Lankoff, S. Azevedo, R. M. Soares","doi":"10.5132/EEC.2013.01.020","DOIUrl":"https://doi.org/10.5132/EEC.2013.01.020","url":null,"abstract":"It is well known that chlorine can oxidize cyanotoxins, thus increasing water potability. Considering that the first steps of conventional treatment do not remove the toxins, the aim of this study was to investigate the toxicity of treated water containing cylindrospermopsin after chlorine addiction. It was analyzed DNA damage and viability of HepG2 cells exposed to the following treatments: cylindrospermopsin (CYN) containing extract of Cylindrospermopsis raciborskii; this same extract added to treated water; non toxic C. raciborskii (all extracts at concentration of 0.1, 0.5 and 1 μg of dry material mL-1), and treated water only. Cells were exposed for 24, 48 and 72 hours. A decrease in cell viability of HepG2 cells was observed after the treatment with toxic C. raciborskii extract (at 0.5 and 1 μg mL-1 for all times of exposure) and the toxic extract with treated water at the two higher concentrations at 48 and 72 hours. Comet assays also revealed DNA damage in HepG2 cells under toxic C. raciborskii extract. Data indicated that chlorine can prevent damage to DNA and to cell viability in most of conditions. In conclusion, chlorine addiction in conventional water treatment has a potential to provide protection or reduce toxic effects of CYN.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129874951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bioensaios para avaliação da toxicidade aguda, reprodução e ganho de biomassa de minhocas (Eisenia fetida) ambientadas em lodo de esgoto doméstico","authors":"Paulo R. Dores-Silva, M. D. Landgraf, M. Rezende","doi":"10.5132/EEC.2013.01.021","DOIUrl":"https://doi.org/10.5132/EEC.2013.01.021","url":null,"abstract":"Este trabalho tem por objetivo avaliar os efeitos que o lodo de esgoto domestico produz na toxicidade, reproducao e ganho de biomassa em minhocas Eisenia fetida. Os resultados obtidos demonstraram que nem o LED nem tampouco o LED diluido em solo apresentam toxicidade aguda para as minhocas sendo que estas tiveram um ganho de biomassa durante os primeiros 14 dias de exposicao ao residuo; no entanto apos este periodo, os organismos tenderam a perder massa.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129949592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Population Approach in Ecotoxicology (Stress Ecology)","authors":"M. N. Flynn, W. R. Pereira","doi":"10.5132/EEC.2013.01.011","DOIUrl":"https://doi.org/10.5132/EEC.2013.01.011","url":null,"abstract":"Several lines of evidence suggest that the effects of stressors on populations can be calculated accurately by population modeling. Using monitoring data of natural population, population models were applied and the possibility of establishing environmental diagnoses from strictly ecological concepts is discussed. A procedure is offered for the modeling of natural population data and the establishment of population endpoints.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127670645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Riscos à biota aquática pelo uso de tintas anti-incrustantes nos cascos de embarcações","authors":"T. L. Martins, V.M.F. Vargas","doi":"10.5132/EEC.2013.01.001","DOIUrl":"https://doi.org/10.5132/EEC.2013.01.001","url":null,"abstract":"The ship hulls are constantly subject to marine biofouling, wich results in a significantly diminution in operational efficiency, rising the costs due to the increase of fuel consumption. From the antifouling systems developed to prevent this problem, those whom present organotin compounds in their formulations became the center of discussions among environmental organs in the last decades. These compounds are considered a threat to marine life in areas with intense naval activity and can act as endocrine disruptors in invertebrates. After restrictions imposed on the use of organotin antifouling paints, others biocides have been used. However, these products also have adverse effects on marine ecosystems. This article reviews the literature related to the risks of the exposure of aquatic organisms to antifouling paints compounds, as well as the alternatives proposed to replace the products yet commercialized.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125526619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}