Journal of The Brazilian Society of Ecotoxicology最新文献_第3页

What is the best buffer for preservation of cells in vitro: a standardization for gill cells in order to use in the Comet Assay 什么是体外保存细胞的最佳缓冲液:用于彗星试验的鳃细胞的标准化

Journal of The Brazilian Society of Ecotoxicology Pub Date : 2013-06-18 DOI: 10.5132/EEC.2013.01.004

N. C. Ghisi, W. Ramsdorf, M. Cestari

{"title":"What is the best buffer for preservation of cells in vitro: a standardization for gill cells in order to use in the Comet Assay","authors":"N. C. Ghisi, W. Ramsdorf, M. Cestari","doi":"10.5132/EEC.2013.01.004","DOIUrl":"https://doi.org/10.5132/EEC.2013.01.004","url":null,"abstract":"The Comet Assay is a rapid and sensitive method for detection of breaks in the DNA strand into individual cells, commonly used at ecotoxicology. At this time, the method has been refined, but not yet fully standardized and variations on protocol are common. When environmental reviews are performed, the large number of samples required and the conditions of handling during transportation of samples to the laboratory are frequent problems. Until now, however, no truly effective method for samples preservation for this test was described. Therefore, we tested three stock solutions: fetal bovine serum (FBS), phosphate buffered saline (PBS) e Tris. Gill cells of Rhamdia quelen contaminated with insecticide Fipronil were maintained in these solutions, for 0, 24 and 48 hours to perform Comet Assay. It was found that fetal bovine serum was the solution that best preserved the DNA integrity of gill cells, followed by Tris and finally by PBS. We concluded in this study that fetal bovine serum is the solution that best conserve the gill cells and genetic material of R. quelen for a time until 48 hours in the absence of light and at 4oC.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126138164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Changes in the activities of glutathione-S-transferases, glutathione reductase and catalase after exposure to different concentrations of cadmium in Australoheros facetus (Cichlidae, Pisces) 不同浓度镉对面南须鱼谷胱甘肽- s转移酶、谷胱甘肽还原酶和过氧化氢酶活性的影响

Journal of The Brazilian Society of Ecotoxicology Pub Date : 2013-06-18 DOI: 10.5132/EEC.2013.01.003

Andrea C. Crupkin, M. Menone

{"title":"Changes in the activities of glutathione-S-transferases, glutathione reductase and catalase after exposure to different concentrations of cadmium in Australoheros facetus (Cichlidae, Pisces)","authors":"Andrea C. Crupkin, M. Menone","doi":"10.5132/EEC.2013.01.003","DOIUrl":"https://doi.org/10.5132/EEC.2013.01.003","url":null,"abstract":"The goal of this work was to evaluate the response of the glutathione-S-transferases (GST), glutathione reductase (GR) and catalase (CAT) enzymes in different tissues of the fish Australoheros facetus exposed cadmium (Cd). In liver, a significant decrease of GST activity was observed at 0.031 mg L-1 (p<0.05), while, at higher concentrations, there were non-significant changes compared to controls. Neither the GR nor the CAT activity was different from control. Gills was the most sensitive organ, showing increased GST activity at 0.31 and 1.53 mg L-1 and GR at 3.06 mg L-1; while CAT activity was inhibited at 0.31, 3.06 and 6.13 mg L-1 (p<0.05). The brain GST activity was increased at 1.53 mg L-1 but inhibited at 3.06 and 6.13 mg L-1, meanwhile GR and CAT activities were inhibited at 1.2, 3.06 and 6.13 mg L-1 (p<0.05). The induction of defense enzymes as well as the inhibition of the antioxidant enzyme catalase, even at environmentally relevant concentrations used in this work (e.g. 0.31 mg L-1), denotes the toxic effect that cadmium exerts over the tissues of A. facetus and alerts over the need of doing biomonitoring in areas potentially polluted with metals.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130260551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Efeito Mutagênico do Edetato de Cobre ([Cu(EDTA)]-2) Livre e Nanoencapsulado em Camundongos e Peixes 游离和纳米胶囊乙酸铜([Cu(EDTA)]-2)对小鼠和鱼类的诱变作用

Journal of The Brazilian Society of Ecotoxicology Pub Date : 2013-06-18 DOI: 10.5132/EEC.2013.01.002

Ana Cristina Jacacobowski, Natalia Novak Zobiole, P. M. Padilha, Susana Elisa Moreno, Eduardo José de Arruda

{"title":"Efeito Mutagênico do Edetato de Cobre ([Cu(EDTA)]-2) Livre e Nanoencapsulado em Camundongos e Peixes","authors":"Ana Cristina Jacacobowski, Natalia Novak Zobiole, P. M. Padilha, Susana Elisa Moreno, Eduardo José de Arruda","doi":"10.5132/EEC.2013.01.002","DOIUrl":"https://doi.org/10.5132/EEC.2013.01.002","url":null,"abstract":"Estudos recentes demonstram que o cobre complexado com o acido etilenodiaminotetracetico, ([Cu(EDTA)]-2), apresenta letalidade sobre larvas de Aedes aegypti. Este composto pode ter uma liberacao lenta e gradativa quando complexado em nanocapsulas de goma/gelatina/quitosana, o que pode limitar sua toxicidade para o ambiente. No entanto, a genotoxicidade do [Cu(EDTA)]-2 ainda nao esta totalmente elucidada. Para avaliar o potencial mutagenico do [Cu(EDTA)]-2, analisamos a frequencia de micronucleos no sangue periferico de camundongos e peixes submetidos a [Cu(EDTA)]-2 livre e nanoencapsulado. A carcaca dos peixes foi avaliada por espectrometria de massa para quantificar o cobre tecidual. Em camundongos a frequencia de micronucleos nos animais expostos ao [Cu(EDTA)]-2 na forma livre foi significativamente maior em relacao aos animais submetidos a forma nanoencapsulada. Em peixes, somente o [Cu(EDTA)]-2 livre foi capaz de induzir aumento na frequencia de micronucleos. A analise espectrometrica demonstrou acumulo de ions cobre no figado nos grupos de peixes estudados, com diferenca significativa em relacao ao grupo controle. Nas brânquias dos peixes nao foram observadas diferencas na concentracao de cobre entre os grupos e ainda, o conteudo de cobre foi significativamente menor em relacao ao encontrado no figado. Os resultados sugerem que o [Cu(EDTA)]-2 livre pode induzir efeitos mutagenicos em mamiferos, porem o nanoencapsulamento parece conferir maior seguranca ao produto devido a sua liberacao mais lenta.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121071126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Estudo sazonal de metais pesados no sedimento do Saco do Laranjal – Pelotas-RS Laranjal - Pelotas-RS沉积物中重金属的季节性研究

Journal of The Brazilian Society of Ecotoxicology Pub Date : 2012-12-05 DOI: 10.5132/JBSE.2012.02.012

Glauco Rasmussen Betemps, P. J. S. Filho

{"title":"Estudo sazonal de metais pesados no sedimento do Saco do Laranjal – Pelotas-RS","authors":"Glauco Rasmussen Betemps, P. J. S. Filho","doi":"10.5132/JBSE.2012.02.012","DOIUrl":"https://doi.org/10.5132/JBSE.2012.02.012","url":null,"abstract":"O Saco do Laranjal e referido pela Base de Dados Tropicais (BDT) como uma area prioritaria para a preservacao. Pouco se conhece sobre a geoquimica dos sedimentos da regiao de estudo, tornando-se indispensavel para as acoes de monitoramento a determinacao de metais pesados no sedimento. Nesse contexto, foi desenvolvido o estudo dos metais pesados como Cu, Pb, Zn e Cr no sedimento do Saco do Laranjal, porcao estuarina da Laguna dos Patos. A amostragem percorreu seis pontos do local de estudo, nos periodos de inverno e primavera de 2009 e verao de 2010. As amostras foram extraidas em triplicata com agua regia a 50% e acido perclorico e analisadas atraves de espectrometria de absorcao atomica em chama. Neste estudo tambem foram avaliados a materia orgânica por metodo indireto atraves da perda de volateis e, granulometria. Os resultados demonstram baixos indices de contaminacao comparados com o Guia de Qualidade para Sedimentos Estuarinos Canadense, apresentando estreita relacao entre o aumento da materia orgânica no teor de finos (fracao < 63 μm) e a concentraca dos metais. Palavras chave : estuario, sazonalidade, materia orgânica, analise de metais.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"120 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114446199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

DNA damage of erythrocytes of fish Oreochromis niloticus (Perciformes, Cichlidae), after acute exposure to river water receiving effluent from an oil refinery 急性暴露于接受炼油厂污水的河水后，尼罗鱼红细胞的DNA损伤(鲈形目，姬鱼科)

Journal of The Brazilian Society of Ecotoxicology Pub Date : 2012-12-05 DOI: 10.5132/JBSE.2012.02.003

Tatiana da Silva Souza, C. S. Fontanetti

引用次数: 5

Genotoxicity evaluation of tilapia (Oreochromis niloticus) exposed to waters from two sites of Itajaí-Açu River (SC, Brazil) 罗非鱼(Oreochromis niloticus)暴露于Itajaí-Açu河两个站点水的遗传毒性评价(SC，巴西)

Journal of The Brazilian Society of Ecotoxicology Pub Date : 2012-12-05 DOI: 10.5132/JBSE.2012.02.008

A. Bücker, M. Conceição

{"title":"Genotoxicity evaluation of tilapia (Oreochromis niloticus) exposed to waters from two sites of Itajaí-Açu River (SC, Brazil)","authors":"A. Bücker, M. Conceição","doi":"10.5132/JBSE.2012.02.008","DOIUrl":"https://doi.org/10.5132/JBSE.2012.02.008","url":null,"abstract":"The relationship between the genes and the effects attributed to them has been object of many studies, especially those seeking to establish the response of genes to environmental prod. The aim of this work was to establish a standard system to monitor effluents by using juvenile Nile tilapia ( Oreochromis niloticus ) as a bioindicator of genotoxicity, utilizing micronuclei test and comet assay. For this, the fish were exposed during 24, 48, 72 and 240 hours (10 days), to water samples collected at two sites of the Itajai-Acu River: Ilhota and Blumenau, in the Santa Catarina State, Brazil. For positive control the herbicide 2,4-D (75 ppm) was utilized, and the negative control of each fish were the values obtained before the exposure to the river water, termed time zero (T 0 ). Water samples from both sites of the Itajai-Acu River showed significant genotoxic effects in erythrocytes of the exposed fishes. The comet assay was a more sensitive test to detect genotoxic damage in shorter exposure times (24 and 48 hours) than the micronuclei test. Key words : genotoxicity, comet assay, micronuclei test, Oreochromis niloticus , bioindicator","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125571882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Mercury distribution in muscular tissues of a tropical carnivorous fish (Hoplias malabaricus) from four lakes in the North of Rio de Janeiro State, SE Brazil 巴西东南部里约热内卢州北部四个湖泊热带食肉鱼(malabaricus Hoplias)肌肉组织中的汞分布

Journal of The Brazilian Society of Ecotoxicology Pub Date : 2012-12-05 DOI: 10.5132/JBSE.2012.02.006

T. B. Jesus, C. Carvalho, Alexandre Gomes Ferreira, E. Siqueira, A. L. Machado

引用次数: 5

Assessment of chromium bioaccumulation in Pseudokirchneriella subcapitata (Korshikov) Hindak by the Central Composite Design (CCD) and Response Surface Methodology (RSM) 中心复合设计与响应面法评价印度拟柯氏菌(Pseudokirchneriella subcapitata (Korshikov) Hindak)体内铬的生物积累

Journal of The Brazilian Society of Ecotoxicology Pub Date : 2012-12-05 DOI: 10.5132/JBSE.2012.02.007

P. C. Giloni-Lima, D. Delello, M. L. Cremonez, M. N. Eler, V. Lima, E. Espíndola

引用次数: 2

Toxicidade e risco ambiental da oxitetraciclina e efeito em leucócitos de mato grosso (Hyphessobrycon eques) 土霉素的毒性、环境风险及对马托格罗索白细胞的影响

Journal of The Brazilian Society of Ecotoxicology Pub Date : 2012-12-05 DOI: 10.5132/JBSE.2012.02.002

R. Y. Fujimoto, M. I. Gabbay, E. C. Anjos, S. P. Carraschi, C. Cruz

{"title":"Toxicidade e risco ambiental da oxitetraciclina e efeito em leucócitos de mato grosso (Hyphessobrycon eques)","authors":"R. Y. Fujimoto, M. I. Gabbay, E. C. Anjos, S. P. Carraschi, C. Cruz","doi":"10.5132/JBSE.2012.02.002","DOIUrl":"https://doi.org/10.5132/JBSE.2012.02.002","url":null,"abstract":"A oxitetraciclina (OTC) e um dos antibioticos mais utilizados para o tratamento de bacterioses de peixes. Os objetivos deste trabalho foram avaliar a toxicidade aguda e o risco ambiental e seus efeitos em leucocitos de mato grosso. Os peixes foram expostos a 2,0; 4,0; 6,0 e 8,0 mg L -1 da OTC e um grupo utilizado como controle. Os testes foram conduzidos em um delineamento inteiramente casualizado, com quatro repeticoes e ao final do experimento, foram coletadas amostras de sangue para contagem dos leucocitos. Para a classificacao do risco ambiental foi utilizada a CL 50 e a concentracao ambiental estimada (CAE) do antibiotico. A concentracao letal 50% (CL(I) 50 ;96h) estimada da OTC foi 5,49 mg L -1 com limite inferior de 5,08 mg L -1 e superior de 5,95 mg L -1 . A OTC causa moderado risco de intoxicacao ambiental com a menor CAE e alto risco de intoxicacao ambiental com a maior CAE. Nao ocorreu diferenca significativa na porcentagem de eosinofilos, celula granulocitica especial e basofilos, porem ocorreu linfocitopenia e monocitose em 4 mg L -1 . Assim, a OTC e moderadamente toxica e causa risco de intoxicacao ambiental para o mato grosso e pode causar estresse em 4,0 e imunossupressao em 6,0 mg L -1 . Palavras-chaves : Peixe, Antibiotico, Concentracao letal, Hematologia.","PeriodicalId":163251,"journal":{"name":"Journal of The Brazilian Society of Ecotoxicology","volume":"85 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2012-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121496864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Effects of sodium and potassium on life history parameters of freshwater cladoceran Pseudosida ramosa 钠和钾对淡水枝蛤生活史参数的影响

Journal of The Brazilian Society of Ecotoxicology Pub Date : 2012-12-05 DOI: 10.5132/JBSE.2012.02.013

E. C. Freitas, O. Rocha

引用次数: 5