Journal of Liposome Research最新文献_第8页

Preparation, physicochemical evaluation and in vitro toxicity studies of HSPC and HSPC:DMPC stigmasterol-loaded liposomes. HSPC和HSPC:DMPC负载豆甾醇脂质体的制备、理化评价及体外毒性研究。

IF 4.3 4区医学

Journal of Liposome Research Pub Date : 2025-12-01 Epub Date: 2025-05-28 DOI: 10.1080/08982104.2025.2502928

Anna-Maria Gaber, Maria Tsakiri, Hector Katifelis, Maria Gazouli, Costas Demetzos

{"title":"Preparation, physicochemical evaluation and in vitro toxicity studies of HSPC and HSPC:DMPC stigmasterol-loaded liposomes.","authors":"Anna-Maria Gaber, Maria Tsakiri, Hector Katifelis, Maria Gazouli, Costas Demetzos","doi":"10.1080/08982104.2025.2502928","DOIUrl":"10.1080/08982104.2025.2502928","url":null,"abstract":"Phytosterols, like stigmasterol, have been studied for their antioxidant, immunomodulatory, and anticancer properties. However, their lipophilic nature and biological instability make it challenging to incorporate them in food supplements and medicinal products. Liposomes offer many benefits in sterols' entrapment and delivery them due to their high bioavailability, low toxicity, and ability to target specific tissues. The purpose of this study was to develop stigmasterol-loaded liposomes using HSPC (Hydrogenated Soy Phosphatidylcholine) and HSPC:DMPC (Dimyristoylphosphatidylcholine). The impact of increasing stigmasterol concentrations on the physicochemical stability of the liposomal formulations was analyzed by dynamic light scattering. The results showed that HSPC-based liposomes could incorporate higher amounts of stigmasterol compared to the HSPC:DMPC-based liposomes. Further analysis through differential scanning calorimetry revealed the formation of metastable phases in HSPC:DMPC:stigmasterol lipid bilayers. Finally, an in vitro MTS assay on HEK-293 cells demonstrated the low toxicity of the stigmasterol-loaded nanoplatforms. In conclusion, stigmasterol, not only contributed to the stability of liposomal formulation but exhibited low cell toxicity on HEK-293 line and could be used as a valuable compound in liposomal drug delivery formulation.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"345-356"},"PeriodicalIF":4.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Triple co-culture spheroid models of lung and ovarian carcinoma cell lines for the in vitro testing of antitumor liposomes. 肺癌和卵巢癌细胞系三重共培养球形模型体外抗肿瘤脂质体检测。

IF 4.3 4区医学

Journal of Liposome Research Pub Date : 2025-12-01 Epub Date: 2025-06-05 DOI: 10.1080/08982104.2025.2514850

Shrey Shah, Yongwoon Kim, Rock Pulak, Gerard G M D'Souza

{"title":"Triple co-culture spheroid models of lung and ovarian carcinoma cell lines for the in vitro testing of antitumor liposomes.","authors":"Shrey Shah, Yongwoon Kim, Rock Pulak, Gerard G M D'Souza","doi":"10.1080/08982104.2025.2514850","DOIUrl":"10.1080/08982104.2025.2514850","url":null,"abstract":"Tumor cells cultured as spheroids have been shown to be superior to tumor cells cultured in monolayers as in vitro models of solid tumors because they exhibit features of the tumor microenvironment (TME) such as cell-cell interactions, extracellular matrix and diffusional gradients. However, spheroids composed solely of tumor cells, i.e. monoculture spheroids, still lack the non-tumor cell components that contribute to additional in vivo TME complexity. This study, explored the development of triple co-culture spheroid models incorporating tumor cells, tissue specific fibroblasts, and endothelial cells to mimic more of the features of the in vivo TME. Using a modified liquid overlay technique, triple co-culture spheroids were successfully generated for both drug resistant lung tumor cells as well as drug resistant ovarian tumor cells. The triple co-culture models exhibited several characteristics of in vivo tumors, including extracellular matrix (ECM) production and distinct spatial locations of cell types. Notably, fibroblasts remained in the core as the spheroid grew, while endothelial cells were found in the core only in the presence of fibroblasts. A liposomal formulation previously shown in monolayer cultures to have selective toxicity toward multiple drug resistant tumor cell types was significantly less toxic and showed composition-dependent levels of toxicity in spheroid cultures with multiple cell types. These findings demonstrate that triple co-culture spheroids can serve as in vitro models that more closely mimic in vivo tumor characteristics to facilitate the optimization of antitumor therapies prior to in vivo testing.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"393-405"},"PeriodicalIF":4.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and immunoactivity of sulfated glucan from Saccharomyces cerevisiae liposomes. 酿酒酵母脂质体硫酸酸化葡聚糖的制备及其免疫活性研究。

IF 4.3 4区医学

Journal of Liposome Research Pub Date : 2025-12-01 Epub Date: 2025-07-06 DOI: 10.1080/08982104.2025.2527096

Yijiong Tao, Linjie Huang, Zhaolong Li, Jiayi Li, Qi Tang, Kai Chen, Lifang Zhang, Chenzhong Fei, Yinchun Liu, Mi Wang

{"title":"Preparation and immunoactivity of sulfated glucan from Saccharomyces cerevisiae liposomes.","authors":"Yijiong Tao, Linjie Huang, Zhaolong Li, Jiayi Li, Qi Tang, Kai Chen, Lifang Zhang, Chenzhong Fei, Yinchun Liu, Mi Wang","doi":"10.1080/08982104.2025.2527096","DOIUrl":"10.1080/08982104.2025.2527096","url":null,"abstract":"This study optimized the preparation conditions of sulfated glucan from Saccharomyces cerevisiae liposomes (SGSCL) and evaluated its effect on immune activity. SGSCL was prepared using the reverse evaporation method, and its immune activity was assessed by measuring the proliferation of chicken spleen lymphocytes, hemagglutination inhibition (HI) antibody titers, and serum cytokine concentrations in chickens vaccinated with the Newcastle disease (ND) vaccine. The optimal preparation conditions were a phospholipid-to-cholesterol mass ratio of 5.4:1, a phospholipid-to-SGSC mass ratio of 10:1, and a rotary evaporation temperature of 40 °C. The average encapsulation efficiency (EE) was 63.92%, whereas the mean particle size, polymer dispersity index (PDI), and zeta potential were 90.39 ± 1.71 nm, 0.203 ± 0.004, and -41.13 ± 1.05 mV, respectively. SGSCL significantly promoted the proliferation of chicken spleen lymphocytes, splenic T and B lymphocytes at concentrations of 100-800 µg/mL, 800 µg/mL and 200-800 µg/mL in vitro. The best proliferative effect on splenic lymphocytes were at 400 µg/mL, 800 µg/mL, and 800 µg/mL. In vivo, on days 7 and 14, HI antibody titers in the SGSCL-H, SGSCL-M, and SGSCL-L groups were significantly greater than those in the VC group. The serum antibody titers in the SGSCL-H and SGSCL-M groups were significantly or numerically elevated compared to the VC group at all time points post-vaccination. The IL-2, IL-6, IL-4, and IFN-γ concentrations in the SGSCL-H group were significantly higher than that in the VC group on D28 and D35. These findings suggest that SGSCL could serve as a novel vaccine diluent or immune adjuvant.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"469-480"},"PeriodicalIF":4.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liposome stability: multifactorial regulation and optimization strategies in in vivo delivery. 脂质体稳定性：体内给药的多因子调控和优化策略。

IF 4.3 4区医学

Journal of Liposome Research Pub Date : 2025-12-01 Epub Date: 2025-09-21 DOI: 10.1080/08982104.2025.2561825

Xiao-Si Lu, Li-Qing Yang, Lan Ma, Jiang-Feng Wu, Hong-Bing Zhang, Ying Wang

{"title":"Liposome stability: multifactorial regulation and optimization strategies in in vivo delivery.","authors":"Xiao-Si Lu, Li-Qing Yang, Lan Ma, Jiang-Feng Wu, Hong-Bing Zhang, Ying Wang","doi":"10.1080/08982104.2025.2561825","DOIUrl":"10.1080/08982104.2025.2561825","url":null,"abstract":"As an essential carrier for drug delivery systems, the stability of liposomes directly influences the efficiency and safety of drug delivery. In the complex and challenging process of in vivo delivery, the stability of liposomes is affected by both their inherent properties and external environmental factors. This paper first introduces the in vivo journey of liposomes, encompassing blood circulation, tissue distribution, metabolism, and excretion. It then summarizes the biological factors affecting liposome stability, including immune recognition, protein-lipid interactions, enzyme-catalyzed degradation, and physiological changes. Additionally, the paper explores effective strategies to enhance liposome stability, such as optimizing lipid composition and surface modification. Finally, it discusses future research directions and existing challenges, focusing on AI-assisted liposome development, and the development of biocompatible materials. The study emphasizes the critical factors affecting liposome stability, uncovers current limitations, and highlights future potential in this field, providing a theoretical foundation and practical guidance for the design and application of liposomes.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"607-618"},"PeriodicalIF":4.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro cytotoxicity of liposomal stearyl triphenyl phosphonium is influenced by mitochondrial bioenergetics. 脂质体硬脂酰三苯磷的体外细胞毒性受线粒体生物能量学的影响。

IF 4.3 4区医学

Journal of Liposome Research Pub Date : 2025-12-01 Epub Date: 2025-10-03 DOI: 10.1080/08982104.2025.2567860

Shrey Shah, Rajitha Gadde, Maitri Shukla, Hiral Tanna, Purvajeet P Jadeja, Himani Bhagwat, Ruchita Joshi, Snigdha Suluru, Gerard G M D'Souza

{"title":"In vitro cytotoxicity of liposomal stearyl triphenyl phosphonium is influenced by mitochondrial bioenergetics.","authors":"Shrey Shah, Rajitha Gadde, Maitri Shukla, Hiral Tanna, Purvajeet P Jadeja, Himani Bhagwat, Ruchita Joshi, Snigdha Suluru, Gerard G M D'Souza","doi":"10.1080/08982104.2025.2567860","DOIUrl":"10.1080/08982104.2025.2567860","url":null,"abstract":"Stearyl triphenyl phosphonium (STPP) has previously been utilized to prepare mitochondriotropic liposomes for improved drug delivery to mitochondria. Additionally, liposomal STPP has shown higher toxicity toward drug-resistant tumor cells compared to non-drug-resistant tumor cells. The differential toxicity is hypothesized to be due to the differences in mitochondrial bioenergetics between drug-resistant and non-drug-resistant cells. This study aimed to further test the hypothesis by using drug-resistant and non-drug-resistant cell lines from ovarian, lung, uterine and breast carcinomas. Mitochondrial function was assessed by JC-1 dye accumulation and by measurement of oxygen consumption rate. Liposomal STPP cytotoxicity was then assessed using a metabolic assay. The drug-resistant ovarian, lung and uterine carcinoma cell lines showed higher mitochondrial activity compared to their non-drug-resistant counterparts while there was no difference in mitochondrial activity between drug-resistant and non-drug-resistant breast carcinoma cell lines. Consistent with the hypothesis of the study, liposomal STPP showed greater toxicity to the drug-resistant ovarian and uterine carcinoma cell lines compared to the non-drug-resistant cell lines and no difference in toxicity between drug-resistant and non-drug-resistant breast carcinoma cell lines. While liposomal STPP was more toxic toward the non-drug-resistant cell line in the case of lung carcinoma, the effect is likely due to the use of altered growth conditions for the non-drug-resistant cell line that were needed to allow testing by the assays used in the study. Collectively, the results suggest the potential for exploiting mitochondrial bioenergetic differences to target drug-resistant and non-drug-resistant tumor cell populations in a variety of tumor types.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"516-521"},"PeriodicalIF":4.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-drug-loaded magnetic lipid based nanobubbles are effective in non-small cell lung cancer treatment: in vitro and in vivo studies. 双重药物负载磁性脂基纳米泡在非小细胞肺癌治疗中有效：体外和体内研究。

IF 4.3 4区医学

Journal of Liposome Research Pub Date : 2025-12-01 Epub Date: 2025-10-16 DOI: 10.1080/08982104.2025.2567866

Güliz Ak, Habibe Yılmaz, Ümmühan Fulden Aydın, Ayşe Ünal, Özge Sarı Turgut, Murat Yalçın, Fatih Oltulu, Altuğ Yavaşoğlu, Nefise Ülkü Karabay Yavaşoğlu, Ayşe Nalbantsoy, Hayal Boyacıoğlu, Buket Bakan, Yeliz Yıldırım, Çiğdem Çelen, Şenay Hamarat Şanlıer

{"title":"Dual-drug-loaded magnetic lipid based nanobubbles are effective in non-small cell lung cancer treatment: in vitro and in vivo studies.","authors":"Güliz Ak, Habibe Yılmaz, Ümmühan Fulden Aydın, Ayşe Ünal, Özge Sarı Turgut, Murat Yalçın, Fatih Oltulu, Altuğ Yavaşoğlu, Nefise Ülkü Karabay Yavaşoğlu, Ayşe Nalbantsoy, Hayal Boyacıoğlu, Buket Bakan, Yeliz Yıldırım, Çiğdem Çelen, Şenay Hamarat Şanlıer","doi":"10.1080/08982104.2025.2567866","DOIUrl":"10.1080/08982104.2025.2567866","url":null,"abstract":"The high rate of lung cancer and related deaths necessitated new treatment strategies. This study aimed at in vitro, in vivo, and ex vivo evaluation of magnetic field sensitive, ultrasound-mediated, and pemetrexed and pazopanib containing nanobubble (NB) destruction to provide dual drug therapy against non-small cell lung cancer. For this purpose, cytotoxicity analysis (CRL-5807, CRL-5826, A549-Luc-C8), biocompatibility experiments including hemocompatibility, uptake by macrophages, and binding to serum proteins, acute and long-term toxicity analyses, in vivo therapeutic efficacy experiments, pharmacokinetic analyses, histochemistry, and immunohistochemistry experiments were performed on NBs. The IC50 value of NB-400 against A549-Luc-C8 was found to be 31.4 ± 1.6 μg/mL and 34.9 ± 1.2 μg/mL with and without ultrasound application for 72 h, respectively. 30.8% of NB-400 was phagocytosed by macrophage cells and was non-hemolytic. It was determined that NB-800 (prepared for inhaler administration) and NB-400 did not cause acute or long-term toxic effects and reduced tumor size and/or disappeared after treatment, but carrier-free dual drugs did not give a therapeutic response also based on immunohistochemical analyses. It can be said that the emerging intravenous and inhaled delivery systems can be potential therapeutics that can be used in the treatment of non-small cell lung cancer with superior properties such as magnetic targeting and ultrasound sensitivity.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"522-535"},"PeriodicalIF":4.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145301372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRA/MEL immunoliposomes act as a targeted medicine in BT-474 breast cancer cells. TRA/MEL免疫脂质体可作为靶向药物治疗BT-474乳腺癌细胞。

IF 4.3 4区医学

Journal of Liposome Research Pub Date : 2025-12-01 Epub Date: 2025-05-21 DOI: 10.1080/08982104.2025.2505102

Sajjad Hamze Mostafavi, Sahar Mohammadi, Fahimeh Sadat Mousavi Alborzi, Fahimeh Hajiahmadi, Davoud Ahmadvand, Nematollah Gheibi, Hossein Naderi-Manesh, Hanifeh Shariatifar, Alireza Farasat

{"title":"TRA/MEL immunoliposomes act as a targeted medicine in BT-474 breast cancer cells.","authors":"Sajjad Hamze Mostafavi, Sahar Mohammadi, Fahimeh Sadat Mousavi Alborzi, Fahimeh Hajiahmadi, Davoud Ahmadvand, Nematollah Gheibi, Hossein Naderi-Manesh, Hanifeh Shariatifar, Alireza Farasat","doi":"10.1080/08982104.2025.2505102","DOIUrl":"10.1080/08982104.2025.2505102","url":null,"abstract":"Breast cancer is one of the most common and deadly cancers worldwide. Melittin is the main component of bee venom, which has multiple anti-cancer properties. Targeted delivery of the gene encoding melittin using TRA-conjugated immunoliposomes to breast cancer cells can effectively treat this disease and reduce the side effects. Liposomes were prepared using the thin-film hydration method. The conjugation of TRA to liposomes was confirmed using SDS-PAGE, FTIR, and Bradford assay and characterized by DLS and TEM. The MTT, Fluorescent microscopy imaging, and flow cytometry methods were chosen to investigate the cytotoxicity and internalization of MEL/PEG-Lip and TRA/MEL immunoliposomes in the BT-474 cell line. The hydrodynamic diameter of TRA/MEL immunoliposomes was about 156 nm, and their appearance was spherical. The IC50 values for TRA/MEL immunoliposomes were calculated as 7.73 and 5.41 µg/mL for 48 and 72 h, respectively, which indicated that TRA/MEL immunoliposomes had a more significant cytotoxic effect on BT-474 cells than MEL/PEG-Lip. In addition, flow cytometry results showed that TRA/MEL immunoliposomes enter BT-474 cells to a greater extent and cause apoptosis. Due to the ability of TRA/MEL immunoliposomes to target and induce apoptosis in BT-474 cancer cells, this nanostructure can be suggested as a promising alternative in the treatment of this type of breast cancer.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"382-392"},"PeriodicalIF":4.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the remyelinating efficacy of nanoliposomes encapsulating Withania somnifera and Ginkgo biloba in a murine model of induced demyelination. 探讨包封苦参和银杏纳米脂质体对小鼠诱导脱髓鞘的影响。

IF 4.3 4区医学

Journal of Liposome Research Pub Date : 2025-12-01 Epub Date: 2025-06-10 DOI: 10.1080/08982104.2025.2516035

Harshit Saxena, Akhilesh Kumar, Rohit Kumar, Pawan Kumar, Madhu C Lingaraju, Rahul Shukla, Karuna Shankar, Madhu Gupta

{"title":"Exploring the remyelinating efficacy of nanoliposomes encapsulating Withania somnifera and Ginkgo biloba in a murine model of induced demyelination.","authors":"Harshit Saxena, Akhilesh Kumar, Rohit Kumar, Pawan Kumar, Madhu C Lingaraju, Rahul Shukla, Karuna Shankar, Madhu Gupta","doi":"10.1080/08982104.2025.2516035","DOIUrl":"10.1080/08982104.2025.2516035","url":null,"abstract":"Demyelination leads to neuropathies and clinical deficits. Chemical remyelinating agents have variable efficacy and severe undesirable effects. Withania somnifera and Ginkgo biloba are traditionally known for possessing neuroprotective effects. The present study endeavors to reverse the demyelination using Withania somnifera root extract (WNLP) and Ginkgo biloba leaf extract (GNLP) liposomes prepared using thin-film hydration. The mean particle size (89.7 nm for WNLP, 85.5 nm for GNLP), zeta potential (-0.21 mV for WNLP, +0.455 mV for GNLP), and encapsulation efficiency (98.7% for WNLP, 97.5% for GNLP) were recorded. FTIR analysis indicated similar absorption peaks between the crude extracts and nano-liposomal formulations, with slight shifts observed. Scanning electron microscopy confirmed smooth, spherical structures. To evaluate the therapeutic efficacy of oral gavage of WNLP and GNLP in a cuprizone-induced demyelinated mice model, they were randomly divided into groups, namely Control (Healthy, Sham and Vehicle), WNLP (low and high dose), GNLP (low and high dose), Ginkgo biloba crude extract (low and high dose) and Prednisolone. Treatment efficacy was assessed using behavioral tests (SHIRPA, Rota-rod, Hot-plate, NORT, and EPM), hematobiochemical, histology, and immunohistochemical analyses. The GNLP in high doses had significant improvement compared to others, highlighting its potential as a promising therapy for demyelinating neuropathies, paving the way for advancements in neurobiomedical science.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"423-445"},"PeriodicalIF":4.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conjugated crown ether lipid liposomes: enhancing integrity in serum through novel surface modifications. 共轭冠醚脂质体：通过新的表面修饰增强血清的完整性。

IF 4.3 4区医学

Journal of Liposome Research Pub Date : 2025-12-01 Epub Date: 2025-07-07 DOI: 10.1080/08982104.2025.2530003

Athanasios Skouras, Eirini Mallikopoulou, Gerasimos Tsivgoulis, Spyridon Mourtas, Sophia G Antimisiaris

{"title":"Conjugated crown ether lipid liposomes: enhancing integrity in serum through novel surface modifications.","authors":"Athanasios Skouras, Eirini Mallikopoulou, Gerasimos Tsivgoulis, Spyridon Mourtas, Sophia G Antimisiaris","doi":"10.1080/08982104.2025.2530003","DOIUrl":"10.1080/08982104.2025.2530003","url":null,"abstract":"This study explores the potential of 18-crown-6 ether to enhance liposomal integrity in biological media. A novel 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE)-crown ether conjugate was synthesized and incorporated into phosphatidylcholine/cholesterol liposomes at varying concentrations (0.5-8 mol%). The physicochemical characteristics, serum integrity, and response to divalent cations were systematically evaluated. Although, these crown ether-modified liposomes do not provide the same level of protection as PEGylation at 48 hours, they clearly exhibited improved integrity in serum at 24 hours compared to non-pegylated liposomes under the same conditions. Moreover, when exposed to calcium ions (5 mM), crown ether-modified liposomes maintained significantly greater stability levels at 24 h in comparison to unmodified liposomes. Furthermore, co-functionalization with both crown ether (2%) and PEG (2%) lipids resulted in stability identical to 4% PEGylated liposomes under the same conditions, suggesting an additive stabilization effect of crown ether and PEG, at least at the concentration applied. These findings introduce crown ether-modified liposomes as a novel approach to modulate liposome-protein interactions and potentially enhance drug delivery in physiological environments containing high concentrations of calcium.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"481-489"},"PeriodicalIF":4.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formulation, characterization, and in vitro evaluation of lactoferrin conjugated liposome loaded with magnesium sulfate for improved CNS penetrability. 乳铁蛋白偶联脂质体的配方、表征和体外评价：载硫酸镁改善中枢神经系统穿透性。

IF 4.3 4区医学

Journal of Liposome Research Pub Date : 2025-12-01 Epub Date: 2025-09-19 DOI: 10.1080/08982104.2025.2560888

Aparna Ann Mathew, Rajitha Panonnummal, Anand Kumar A, Sabitha M

{"title":"Formulation, characterization, and in vitro evaluation of lactoferrin conjugated liposome loaded with magnesium sulfate for improved CNS penetrability.","authors":"Aparna Ann Mathew, Rajitha Panonnummal, Anand Kumar A, Sabitha M","doi":"10.1080/08982104.2025.2560888","DOIUrl":"10.1080/08982104.2025.2560888","url":null,"abstract":"Magnesium deficiency has been reported in association with various disease conditions, particularly in neurological diseases. The limited clinical use of magnesium sulfate is due to its restricted entry into the Central Nervous system (CNS) through blood brain barrier (BBB) which may consequently result in the peripheral accumulation followed by its toxic effects. The current study is focused on the development of nano formulation of lactoferrin conjugated liposome loaded with magnesium sulfate (LMGPLS) with an aim to improve its CNS permeability. LMGPLS was prepared by thin-film hydration technique followed by the lactoferrin conjugation. The average particle size of the LMGPLS was found to be 273.23 ± 6.7 nm. In vitro drug release studies showed that the drug release started after 15 min and continued up to 5 h from LMGPLS. The parallel artificial membrane permeability assay proved the improved lipid layer permeation of prepared formulation when compared with that of the drug solution. The prepared LMGPLS were found to be haemo-compatible and cyto-compatible when tested with blood as well as with multiple cell lines. The pharmacological effects of the formulations were evaluated by multiple in vitro experiments. The results from in vitro experiments showed that the developed formulation is capable of improving the cell viability in glutamate and H2O2 treated cell lines indicating its antioxidant and anti-excitotoxicity effects. Additionally, the developed liposome showed significant effect on digoxin induced Na+/K+ ATPase inhibition. Good lipid layer penetrability with haemo-compatibility and cytocompatibility warrant that the formulation will be a suitable candidate to increase the CNS delivery of Magnesium.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"503-515"},"PeriodicalIF":4.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0