Journal of Liposome Research最新文献

{"title":"Liposomes applied in healing bacterially infected wounds: a systematic review.","authors":"Tainara Aparecida Nunes Ribeiro, Daniel Crístian Ferreira Soares, Grazielle Aparecida Dos Santos, Maria Caiane Lino de Souza, Brenda Xavier Gonçalves, Paula Fernanda da Silva Valentim, Mariana Oliveira de Paula, Daniela Sachs","doi":"10.1080/08982104.2025.2528086","DOIUrl":"10.1080/08982104.2025.2528086","url":null,"abstract":"<p><p>Concerns regarding bacterial infections and antibiotic resistance development have instigated many researchers to synthesize innovative antibacterial compounds and develop more effective pharmaceutical formulations against microorganisms increasingly adapted to our environment. In special, many efforts have been dedicated to developing pharmaceutical antibacterial formulations to treat and improve wound-healing processes, which still represent a great challenge for common clinical practice today. Liposomal systems remain one of the most extensively studied controlled delivery platforms, and considering the antibacterial context, the present article aims to provide a fast and well-oriented systematic review regarding this issue, aiming to bring to the readers the ultimate data regarding the employment of liposomes in the treatment of bacterial infections and the healing of bacteria-infected wounds. A systematic compilation of results and comparisons was made, seeking to observe the antibacterial and wound-healing behavior of the formulated liposomal systems in selected recent studies. The results obtained revealed impressive results concerning the potential applications of liposomes against bacteria in the wound-healing medical context.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"590-606"},"PeriodicalIF":4.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Folate-targeted long-circulating 5-fluorouracil liposomes: preparation, characterization, anti-tumor effect and pharmacokinetic profile in rats.","authors":"Han Chen, Jin Su, Wenhui Cheng, Linwen Wen, Meiqi Huang, Xuejiao Li, Wei Cai, Qian Ning, Liangjun Guan","doi":"10.1080/08982104.2025.2571625","DOIUrl":"10.1080/08982104.2025.2571625","url":null,"abstract":"<p><p>5-Fluorouracil (5-Fu) is a chemotherapy drug that can treat various cancers. However, its short plasma half-life and severe adverse reactions limit its wide application in clinical utility. To overcome these drawbacks, in this study, 5-Fu long-circulating liposomes (SSL-5-Fu Lip) were prepared by the film hydration method. DSPE-PEG₂₀₀₀-FA was conjugated to the surface of SSL-5-Fu Lip through amidation reaction, resulting in the successful preparation of FA-SSL-5-Fu Lip. The particle size and zeta potential were measured. The encapsulation efficiency of 5-Fu was determined by high-performance liquid chromatography (HPLC), and the drug loading was calculated. The stability of FA-SSL-5-Fu Lip was evaluated at 4 °C based on particle size and PDI. Pharmacokinetic studies were conducted in male Sprague-Dawley (SD) rats. The average particle size of the liposomes was 139.11 ± 3.1 nm, and the zeta potential was -4.30 ± 0.91 mV. The encapsulation efficiency and drug loading of 5-Fu were 76.88 ± 4.23% and 24.14 ± 0.97%, respectively. The half-lives of 5-Fu and FA-SSL-5-Fu Lip were 0.319 h and 1.059 h, respectively. It can be preliminarily proved that FA-SSL-5-Fu Lip has the effect of prolonging circulation time. Compared with free 5-Fu, it prolongs the circulation time of the drug in the body, laying a foundation for its clinical application.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"536-547"},"PeriodicalIF":4.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145292501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.1080/08982104.2025.2499787","DOIUrl":"10.1080/08982104.2025.2499787","url":null,"abstract":"","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"i"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of PEG-modified isoquercitrin liposomes and anti-chronic kidney disease research.","authors":"Qinyang Hua, Qilong Wang, Xue Wang, Xia Jiang, Mingjie Gong, Jiaying Li, Tingyuan Li, Xiaowen Wang, Xia Cao, Jiangnan Yu, Elmurat Toreniyazov, Bin Zong, Ximing Xu, Feng Shi, Michael Adu-Frimpong","doi":"10.1080/08982104.2025.2480782","DOIUrl":"10.1080/08982104.2025.2480782","url":null,"abstract":"<p><p>The clinical application of Isoquercitrin (IQ) is limited by its low water solubility and short retention time in the body, despite its diverse pharmacological effects. To address these issues, we prepared polyethylene glycol (PEG)-modified IQ liposomes (IQ-L) using the thin film dispersion method and optimized the formulation through a combination of One Factor at a Time (OFAT) method and response surface experiments. Characterization of the IQ-L that was prepared using the optimal formulation revealed a particle size of 185.48 nm, a polydispersity index of 0.252, a zeta potential of -33.88 mV, and an impressive encapsulation efficiency of 97.84%. In vitro release studies showed a significantly higher cumulative release rate for IQ-L compared to free IQ. Pharmacokinetic evaluations in rats demonstrated a 4.54-fold increase in the area under the concentration-time curve, a 1.63-fold prolongation of the half-life, and a 2.07-fold increase in peak concentration for IQ-L compared to unmodified IQ. Moreover, assessments of renal function in a mouse model indicated promising therapeutic effects. In summary, the PEG-modified liposome system greatly improved the solubility and in vivo retention time of IQ, thus making it a potential clinical agent for the treatment of chronic kidney disease (CKD).</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"252-266"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liposomes incorporating cyclodextrins as a promising drug delivery system to augment the bioavailability of poorly soluble drugs.","authors":"Manar Adel Abdelbari, Shaimaa Mosallam","doi":"10.1080/08982104.2025.2473335","DOIUrl":"10.1080/08982104.2025.2473335","url":null,"abstract":"<p><p>Poorly water-soluble drugs are common and challenging in pharmaceutical industry. The poor solubility can reduce the drugs' therapeutic efficiency and bioavailability. Improving the solubility and bioavailability of poorly water-soluble drugs is a challenge and a main issue in the development and application of these drugs in pharmaceutical industry. Liposomes is phospholipid-based vesicular drug delivery system which act as drug carriers for both lipophilic or hydrophilic drugs and have the ability to solubilize the carried drugs. An innovative approach is the incorporation of cyclodextrins (CDs) into the liposomes encapsulating the drug molecules for improving safety and effectiveness of drug molecules by the use of water-soluble CDs properties, which allow the formation of CDs liposomes complexes with lipophilic molecules. CDs liposomes complex increases targeting effect, regulates drug release, improves drug properties and drug encapsulation efficiency. The aim of this review is to summarize the advantages and applications of liposomes incorporating CDs to enhance bioavailability of poorly soluble drugs.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"312-320"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of PEGylation for cationic triacyl lipid-based siRNA lipoplexes prepared using the modified ethanol injection method for tumor therapy.","authors":"Yoshiyuki Hattori, Mizuki Shinkawa, Aya Kurihara, Ryohei Shimizu","doi":"10.1080/08982104.2025.2498956","DOIUrl":"10.1080/08982104.2025.2498956","url":null,"abstract":"<p><p>We previously developed a modified ethanol injection (MEI) method to construct small interfering RNA (siRNA) lipoplexes by mixing a lipid-ethanol solution with an siRNA-containing phosphate-buffered saline solution. Here, we constructed siRNA lipoplexes with 11-((1,3-bis(dodecanoyloxy)-2-((dodecanoyloxy)methyl)propan-2-yl)amino)-<i>N</i>,<i>N</i>,<i>N</i>-trimethyl-11-oxoundecan-1-aminium bromide (TC-1-12), 1,2-dioleoyl-<i>sn</i>-glycero-3-phosphoethanolamine, and poly(ethylene glycol) (PEG)-lipid using our MEI method. The siRNA lipoplexes were PEGylated with 1, 3, 5, and 10 mol% PEG cholesteryl ether (PEG-Chol), 1,2-dimyristoyl-<i>rac</i>-glycero-3-methoxypolyethylene glycol (mPEG-DMG), or 1,2-distearoyl-<i>sn</i>-glycero-3-phosphoethanolamine-<i>N</i>-(methoxy[polyethylene glycol]) (mPEG-DSPE). PEGylation of siRNA lipoplexes with PEG-Chol did not attenuate the inhibitory effects of Luc and polo-like kinase 1 (PLK1) siRNA lipoplexes on the luciferase (Luc) activity and proliferation of human cervical carcinoma HeLa-Luc, human ovarian cancer SK-OV-3-Luc, and human breast cancer MCF-7-Luc cells stably expressing Luc. In contrast, PEGylated lipoplexes with 10 mol% mPEG-DMG inhibited Luc activity by Luc siRNA but considerably attenuated the PLK1 siRNA-mediated cytotoxic effects. For PEGylated siRNA lipoplexes with mPEG-DSPE, inhibitory effect of Luc siRNA on Luc activity decreased with increasing amounts of PEG modification, and PLK1 siRNA-mediated cytotoxic effects disappeared at more than 3 mol% PEGylation. Erythrocyte aggregation and hemolysis induction by the siRNA lipoplexes were effectively inhibited by 10 mol% PEGylation, irrespective of the PEG-lipid. Compared to those with 1 mol% PEG-Chol, PEGylated siRNA lipoplexes with 10 mol% PEG-Chol potently reduced siRNA accumulation in mouse lungs post-intravenous administration. Overall, TC-1-12-based siRNA lipoplexes with 10 mol% PEG-Chol exerted PLK1 siRNA-mediated cytotoxic effects, without inducing hemolysis and erythrocyte aggregation.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"300-311"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PEGylated liposomes via ATRP for brain drug delivery.","authors":"Lulu Chen, Zhenfang Zhang, Maria R Simonsen, Trevor Owens, Reza M H Khorooshi, Changzhu Wu","doi":"10.1080/08982104.2025.2485428","DOIUrl":"10.1080/08982104.2025.2485428","url":null,"abstract":"<p><p>PEGylated liposomes play a critical role in drug delivery systems because they can evade immune recognition. However, conventional methods for synthesizing PEGylated liposomes often involve the direct incorporation of PEG-functionalized lipids, resulting in insufficient and inconsistent PEG distribution on the liposome surface, which compromises their stability and performance. In this study, we present a proof-of-concept synthesis approach that utilizes lipid-based initiators to form liposomes, followed by controllable grafting of PEG chains through atom transfer radical polymerization (ATRP). This method ensures controlled and uniform PEG coverage, resulting in improved functionality. Compared to conventional liposomes, the polymer-grafted liposomes synthesized via ATRP demonstrated superior cellular uptake <i>in vitro</i>, enhanced penetration of the blood-brain barrier (BBB), and improved stability <i>in vivo</i>, particularly for protein-encapsulated formulations such as green fluorescent protein (GFP). Live/dead assays confirmed the biocompatibility of the ATRP-synthesized PEGylated liposomes. Therefore, our strategy significantly enhances the efficiency of PEGylated liposomes for targeted brain drug delivery, providing a promising platform for the treatment of neurological disorders.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"283-289"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional liposomal gel in regenerative medicine.","authors":"Meghna Mohandas, Jayakumar Rangasamy","doi":"10.1080/08982104.2025.2480786","DOIUrl":"10.1080/08982104.2025.2480786","url":null,"abstract":"<p><p>The synergistic approach of liposome integrated with gel matrix could reshape the current frameworks of drug delivery technology. The liposome-based approaches are limited by inadequate stability and rapid leakage of drug molecules. Undesired and immediate drug release from gel increases the local concentration of drug and causes toxicity. So, the stabilization of liposomes within a gel matrix can be an effective option to provide an ingenious solution to the conventional limitation on short half-life, instability, toxicity, uncontrolled drug release and poor retention of drug molecules on the target site. The capability to incorporate antibacterial as well as anti-oxidant drugs, antimicrobial peptides, ligands, growth hormones, antigens, and imaging agents had contributed to the establishment of multifunctional liposomal gel system has significant advantage in regenerative medicine area. This review will focus the advantage of multifunctional liposomal gels in context of infectious wound healing, skin rejuvenation, musculoskeletal repair and trauma management, spinal cord injury treatment, tumor specific chemotherapy as well as immunotherapy and vaccination. The versatility in executing the multiple functions will be a valuable solution for advancing the therapeutic outcomes in regenerative medicine.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"321-333"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redox-responsive liposomes aimed at nitroreductase for contents release.","authors":"Brajadulal Ghosh, Robin L McCarley","doi":"10.1080/08982104.2025.2490537","DOIUrl":"10.1080/08982104.2025.2490537","url":null,"abstract":"<p><p>Here, we report a novel stimuli-responsive N-DOPE liposome where the redox-active 4-nitrobenzyl formate head group of liposomes would respond with respect to the presence of nitroreductase present in the environment of tumor tissues to release the payload. Our main emphasis is related to the construction of redox-sensitive liposomes that would function as the liposomal drug carriers to malignant tumors. Our N-DOPE liposome contains a nitro group (-NO₂) in the modified lipid, and we expect the reduction of the nitro group (-NO₂) to amine (-NH₂) would release the calcein (drug) through the 1,6 elimination as per our hypothesis. But we found no release after waiting for almost 20 hours with the use of Na₂S₂O₄, nitroreductase (NTR) and changes of different external environmental conditions, <i>i.e.</i> temperature, aerobic and anaerobic, etc. due to the formation of an azo (R-N = N-R) bond that stops the complete reduction of (-NO₂) all the way down to form amine (-NH₂) to stop releasing the payloads. However, adding an organic group containing nitro during the reduction process with the Na₂S₂O₄ resulted in a 45% release of liposomal content. A detailed study & explanation of the formation of azo bond in our N-DOPE liposome has been shown in a stepwise manner in through various spectroscopic methods, and we have discussed future directions.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"290-299"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin hyalurosomes as a powerful antioxidant for combating skin damage induced by UV radiation.","authors":"Mariam Zewail, Haidy Abbas, Merhan E Ali, Shaimaa Makled","doi":"10.1080/08982104.2025.2484732","DOIUrl":"10.1080/08982104.2025.2484732","url":null,"abstract":"<p><p>Extrinsic skin aging is caused by chronic skin photodamage. The present study aims to inspect the role of nanoencapsulation of melatonin (MEL) in hyalurosomes in combating UVB-induced skin damage to take advantage of the hydrating penetration enhancing and antiaging effects of hyaluronic acid along with the powerful antioxidant effects of MEL. Measurement of particle size, zeta potential, encapsulation efficiency and <i>in vitro</i> MEL release were carried out. The <i>in vivo</i> photoprotective effects of MEL were tested in rats. A histopathological examination was conducted, and antioxidant and anti-inflammatory markers were measured along with estimating the expression of P38 MAPK, P-ERK and P-JNK. Particle size and zeta potential of MEL hyalurosomes were 285.9 nm and -26.3 mV with 95% entrapment efficiency and provided a sustained release profile for 48h. <i>In vivo,</i> results revealed the superior effect of MEL hyalurosomes in protecting skin against UVB-induced damage and reducing the levels of inflammatory mediators like TNF-α and IL6 compared with MEL suspension. However, they had a prominent role in increasing the levels of antioxidants. These findings may be accredited to the effect of nanoencapsulation in enhancing skin penetration and deposition of MEL besides the effect of hyaluronic acid as a powerful antiaging tool.</p>","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"267-282"},"PeriodicalIF":4.3,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}