Journal of Liposome Research最新文献_第7页

A comprehensive review on transethosomes as a novel vesicular approach for drug delivery through transdermal route. 全面综述透硫体作为一种通过透皮途径给药的新型囊泡方法。

IF 4.4 4区医学

Journal of Liposome Research Pub Date : 2024-03-01 Epub Date: 2023-06-20 DOI: 10.1080/08982104.2023.2221354

Minahal Munir, Muhammad Zaman, Muhammad Ahsan Waqar, Huma Hameed, Tehseen Riaz

{"title":"A comprehensive review on transethosomes as a novel vesicular approach for drug delivery through transdermal route.","authors":"Minahal Munir, Muhammad Zaman, Muhammad Ahsan Waqar, Huma Hameed, Tehseen Riaz","doi":"10.1080/08982104.2023.2221354","DOIUrl":"10.1080/08982104.2023.2221354","url":null,"abstract":"Drug delivery through transdermal route is one of the effective methods for the application of drugs. It overcomes many drawbacks which are encountered with the oral route. Moreover, many drugs are not able to pass through the stratum corneum, which is the main barrier for the transdermal drug delivery. Formation of ultra-deformable vesicles (UDVs) is a novel technique for the transdermal applications of the drugs. Transethosomes (TEs), ethosomes, and transferosomes are all part of the UDV. Because of the presence of increased concentrations of ethanol, phospholipids, and edge activators, TEs provide improved drug permeation through the stratum corneum. Because of the elasticity of TEs, drug penetration into the deeper layer of skin also increases. TEs can be prepared using a variety of techniques, including the cold method, hot method, thin film hydration method, and the ethanol injection method. It increases patient adherence and compliance because it is a non-invasive procedure of administering drugs. Characterization of the TEs includes pH determination, size and shape, zeta potential, particle size determination, transition temperature, drug content, vesicle stability, and skin permeation studies. These vesicular systems can be utilized to deliver a variety of medications transdermally, including analgesics, antibiotics, antivirals, and anticancer and arthritis medications. This review aims to describe vesicular approaches that had been used to overcome the barrier for the transdermal delivery of drug and also describes brief composition, method of preparation, characterization tests, mechanism of penetration of TEs, as well as highlighted various applications of TEs in medicine.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"203-218"},"PeriodicalIF":4.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9655598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The presence of uncoated gold nanoparticle aggregates may alter the phase of phosphatidylcholine lipid as evidenced by vibrational spectroscopies. 振动光谱证明，未涂层金纳米粒子聚集体的存在可能会改变磷脂酰胆碱脂质的相位。

IF 4.4 4区医学

Journal of Liposome Research Pub Date : 2024-03-01 Epub Date: 2023-07-26 DOI: 10.1080/08982104.2023.2239905

Lea Pašalić, Qiqian Liu, Petra Vukosav, Tea Mišić Radić, Aicha Azziz, Marjan Majdinasab, Mathieu Edely, Marc Lamy de la Chapelle, Danijela Bakarić

{"title":"The presence of uncoated gold nanoparticle aggregates may alter the phase of phosphatidylcholine lipid as evidenced by vibrational spectroscopies.","authors":"Lea Pašalić, Qiqian Liu, Petra Vukosav, Tea Mišić Radić, Aicha Azziz, Marjan Majdinasab, Mathieu Edely, Marc Lamy de la Chapelle, Danijela Bakarić","doi":"10.1080/08982104.2023.2239905","DOIUrl":"10.1080/08982104.2023.2239905","url":null,"abstract":"Spherical structures built from uni- and multilamellar lipid bilayers (LUV and MLV) are nowadays considered not just as nanocarriers of various kinds of therapeutics, but also as the vehicles that, when coupled with gold (Au) nanoparticles (NPs), can also serve as a tool for imaging and discriminating healthy and diseased tissues. Since the presence of Au NPs or their aggregates may affect the properties of the drug delivery vehicle, we investigated how the shape and position of Au NP aggregates adsorbed on the surface of MLV affect the arrangement and conformation of lipid molecules. By preparing MLVs constituted from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in the presence of uncoated Au NP aggregates found i) both within liposome core and on the surface of the outer lipid bilayer, or ii) adsorbed on the outer lipid bilayer surface only, we demonstrated the maintenance of lipid bilayer integrity by microscopic techniques (cryo-TEM, and AFM). The employment of SERS and FTIR-ATR techniques enabled us not only to elucidate the lipid interaction pattern and their orientation in regards to Au NP aggregates but also unequivocally confirmed the impact of Au NP aggregates on the persistence/breaking of van der Waals interactions between hydrocarbon chains of DPPC.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"113-123"},"PeriodicalIF":4.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9873229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liposome bilayer stability: emphasis on cholesterol and its alternatives. 脂质体双分子层的稳定性：重点是胆固醇及其替代物。

IF 4.4 4区医学

Journal of Liposome Research Pub Date : 2024-03-01 Epub Date: 2023-06-28 DOI: 10.1080/08982104.2023.2226216

Hamdi Nsairat, Abed Alqader Ibrahim, Areej M Jaber, Sharif Abdelghany, Randa Atwan, Naeem Shalan, Hiba Abdelnabi, Fadwa Odeh, Mohamed El-Tanani, Walhan Alshaer

引用次数: 0

A cochleate formulation optimized by D-optimal mixture design enhances oral bioavailability of Revaprazan. 通过 D-最佳混合物设计优化的蜗牛配方提高了雷伐普赞的口服生物利用度。

IF 4.4 4区医学

Journal of Liposome Research Pub Date : 2024-03-01 Epub Date: 2023-05-09 DOI: 10.1080/08982104.2023.2209171

Yoon Tae Goo, Min Song Kim, Ji Yeh Choi, Gi Hyeong Sin, Sun Ho Hong, Chang Hyun Kim, Young Wook Choi

{"title":"A cochleate formulation optimized by D-optimal mixture design enhances oral bioavailability of Revaprazan.","authors":"Yoon Tae Goo, Min Song Kim, Ji Yeh Choi, Gi Hyeong Sin, Sun Ho Hong, Chang Hyun Kim, Young Wook Choi","doi":"10.1080/08982104.2023.2209171","DOIUrl":"10.1080/08982104.2023.2209171","url":null,"abstract":"A cochleate formulation was developed to enhance the oral bioavailability of revaprazan (RVP). Dimyristoyl phosphatidylcholine (DMPC) liposome containing dicetyl phosphate (DCP) successfully formed a cochleate after treatment with CaCl2, whereas that containing sodium deoxycholate did not. Cochleate was optimised using a D-optimal mixture design with three independent variables-DMPC (X1, 70.58 mol%), cholesterol (X2, 22.54 mol%), and DCP (X3, 6.88 mol%)-and three response variables: encapsulation efficiency (Y1, 76.92%), released amount of free fatty acid at 2 h (Y2, 39.82%), and released amount of RVP at 6 h (Y3, 73.72%). The desirability function was 0.616, showing an excellent agreement between the predicted and experimental values. The cylindrical morphology of the optimised cochleate was visualised, and laurdan spectroscopy confirmed the dehydrated membrane interface, showing an increased generalised polarisation value (approximately 0.5) over small unilamellar vesicle of RVP (RVP-SUV; approximately 0.1). The optimised cochleate showed greater resistance to pancreatic enzyme than RVP-SUV. RVP was released in a controlled manner, achieving approximately 94% release in 12 h. Following oral administration in rats, the optimised cochleate improved the relative bioavailability of RVP by approximately 274%, 255%, and 172% compared to RVP suspension, a physical mixture of RVP and the cochleate, and RVP-SUV, respectively. Thus, the optimised cochleate formulation might be a good candidate for the practical development of RVP.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"31-43"},"PeriodicalIF":4.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9801465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive review on lipid nanocarrier systems for cancer treatment: fabrication, future prospects and clinical trials. 全面综述用于癌症治疗的脂质纳米载体系统：制造、未来前景和临床试验。

IF 4.4 4区医学

Journal of Liposome Research Pub Date : 2024-03-01 Epub Date: 2023-05-05 DOI: 10.1080/08982104.2023.2204372

Mohamed Fawzi Kabil, Osama A Badary, Frank Bier, Shaker A Mousa, Ibrahim M El-Sherbiny

{"title":"A comprehensive review on lipid nanocarrier systems for cancer treatment: fabrication, future prospects and clinical trials.","authors":"Mohamed Fawzi Kabil, Osama A Badary, Frank Bier, Shaker A Mousa, Ibrahim M El-Sherbiny","doi":"10.1080/08982104.2023.2204372","DOIUrl":"10.1080/08982104.2023.2204372","url":null,"abstract":"Over the last few decades, cancer has been considered a clinical challenge, being among the leading causes of mortality all over the world. Although many treatment approaches have been developed for cancer, chemotherapy is still the most utilized in the clinical setting. However, the available chemotherapeutics-based treatments have several caveats including their lack of specificity, adverse effects as well as cancer relapse and metastasis which mainly explains the low survival rate of patients. Lipid nanoparticles (LNPs) have been utilized as promising nanocarrier systems for chemotherapeutics to overcome the challenges of the currently applied therapeutic strategies for cancer treatment. Loading chemotherapeutic agent(s) into LNPs improves drug delivery at different aspects including specific targeting of tumours, and enhancing the bioavailability of drugs at the tumour site through selective release of their payload, thus reducing their undesired side effects on healthy cells. This review article delineates an overview of the clinical challenges in many cancer treatments as well as depicts the role of LNPs in achieving optimal therapeutic outcomes. Moreover, the review contains a comprehensive description of the many LNPs categories used as nanocarriers in cancer treatment to date, as well as the potential of LNPs for future applications in other areas of medicine and research.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"135-177"},"PeriodicalIF":4.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9403163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and characterization of novel surface engineered Depofoam: a QbD coupled failure modes and effects analysis risk assessment-based optimization studies. 新型表面工程消泡剂的开发与表征：基于风险评估的 QbD 耦合失效模式与效应分析优化研究。

IF 4.4 4区医学

Journal of Liposome Research Pub Date : 2024-03-01 Epub Date: 2023-05-05 DOI: 10.1080/08982104.2023.2208662

Jebastin Koilpillai, Damodharan Narayanasamy

{"title":"Development and characterization of novel surface engineered Depofoam: a QbD coupled failure modes and effects analysis risk assessment-based optimization studies.","authors":"Jebastin Koilpillai, Damodharan Narayanasamy","doi":"10.1080/08982104.2023.2208662","DOIUrl":"10.1080/08982104.2023.2208662","url":null,"abstract":"This study aimed to design and develop novel surface-engineered Depofoam formulations to extend the drug delivery to the prescribed time. The objectives are to prevent the formulation from burst release, rapid clearance by tissue macrophages, and instability and to analyze the impact of process and material variables in the characteristics of formulations. This work employed a quality-by-design coupled failure modes and effects analysis (FMEA)-risk assessment strategy. The factors for the experimental designs were chosen based on the FMEA results. The formulations were prepared by the double emulsification method followed by surface modification and characterized in terms of critical quality attributes (CQAs). The experimental data for all these CQAs were validated and optimized using the Box-Behnken design. A comparative drug release experiment was studied by the modified dissolution method. Furthermore, the stability of the formulation was also assessed. In addition, the impact of critical material attributes and critical process parameters on CQAs was evaluated using FMEA risk assessment. The optimized formulation method yielded high encapsulation efficiency (86.24 ± 0.69%) and loading capacity (24.13 ± 0.54%) with an excellent zeta potential value (-35.6 ± 4.55mV). The comparative in vitro drug release studies showed that more than 90% of the drug's release time from the surface-engineered Depofoam was sustained for up to 168 h without burst release and ensured colloidal stability. These research findings revealed that Depofoam prepared with optimized formulation and operating conditions yielded stable formulation, protected the drug from burst release, provided a prolonged release, and sufficiently controlled the drug release rate.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"1-17"},"PeriodicalIF":4.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9780326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carboxymethyl chitosan and octadecylamine-coated liposome-containing WPTS: design, optimization, and evaluation. 羧甲基壳聚糖和十八胺包覆脂质体的 WPTS：设计、优化和评估。

IF 4.4 4区医学

Journal of Liposome Research Pub Date : 2024-03-01 Epub Date: 2023-08-18 DOI: 10.1080/08982104.2023.2246057

Nan Wang, Chi Zhang, Jiahui Wu, Dachuan Zhang, Junling Li, A Galvbu, Leimengyuan Tang, Yan Li, Houxier Li, Shuting Tan, Xueyong Wang

{"title":"Carboxymethyl chitosan and octadecylamine-coated liposome-containing WPTS: design, optimization, and evaluation.","authors":"Nan Wang, Chi Zhang, Jiahui Wu, Dachuan Zhang, Junling Li, A Galvbu, Leimengyuan Tang, Yan Li, Houxier Li, Shuting Tan, Xueyong Wang","doi":"10.1080/08982104.2023.2246057","DOIUrl":"10.1080/08982104.2023.2246057","url":null,"abstract":"Liposomes (LPs) are a delivery system for stabilizing pharmaceuticals with limited use due to their propensity to congregate and fuse. A proposed method of addressing these problems is polymer coating. In this study, the potential of octadecylamine (ODA)-coated liposomes and carboxymethyl chitosan (CMCS/ODA-LPs) for enhancing Wacao pentacyclic triterpene saponin (WPTS) transport capacity was investigated. CMCS/ODA-LPs were produced by electrostatic adsorption and thin-film hydration. Response surface methodology (RSM) was employed to enhance the process and encapsulation efficiency (EE) for optimum drug encapsulation efficiency. The synthesized WPTS-CMCS/ODA-LPs were uniformly dispersed in a circular shape, and during 14 days of storage at 4 °C, the particle size and morphology did not significantly change. Vesicle size, zeta potential, polydispersity index (PDI), and entrapment efficiency (%) were 179.1 ± 7.31 nm, -29.6 ± 1.35 mV, 0.188 ± 0.052, and 75.62 ± 0.43, respectively. The hemolysis test revealed that WPTS-CMCS/ODA-LPs were sufficiently biocompatible. Compared to WPTS-LPs, WPTS-CMCS/ODA-LPs consistently showed a much more significant cytotoxic effect on cancer cells. Early and WPTS-CMCS/ODA-LPs-induced apoptosis resulted in almost seven times more cell death than the control. Compared to physiological pH 7.3, the pH-sensitive CMCS coupled LPs increased drug release at acidic pH 6.5. These findings suggest the efficacy of pH-sensitive CMCS/ODA-LPs as a medication delivery method for WPTS.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"124-134"},"PeriodicalIF":4.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10016611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 更正。

IF 4.4 4区医学

Journal of Liposome Research Pub Date : 2024-03-01 Epub Date: 2023-08-10 DOI: 10.1080/08982104.2023.2246116

引用次数: 0

Nose to brain delivery of naringin loaded transniosomes for epilepsy: formulation, characterisation, blood-brain distribution and invivo pharmacodynamic evaluation. 从鼻腔向大脑输送柚皮苷治疗癫痫：配方、特性、血脑屏障分布和体内药效学评估。

IF 4.4 4区医学

Journal of Liposome Research Pub Date : 2024-03-01 Epub Date: 2023-05-22 DOI: 10.1080/08982104.2023.2214619

Isha Gupta, Syeda Nashvia Adin, Mohd Aqil, Mohd Mujeeb

{"title":"Nose to brain delivery of naringin loaded transniosomes for epilepsy: formulation, characterisation, blood-brain distribution and invivo pharmacodynamic evaluation.","authors":"Isha Gupta, Syeda Nashvia Adin, Mohd Aqil, Mohd Mujeeb","doi":"10.1080/08982104.2023.2214619","DOIUrl":"10.1080/08982104.2023.2214619","url":null,"abstract":"The current work limns the preparation of naringin-loaded transnioosomes (NRN-TN) to enhance NRN solubility, permeation and bioavailability via nasal mucosa for intranasal delivery. NRN-TN was created by the thin-film hydration technique, and with the BBD (Box-Behnken design), optimisation was carried out. NRN-TNopt was characterised for the vesicle size, PDI (Polydispersity index), zeta potential, entrapment efficiency (EE) and in vitro NRN release. For further assessment, nasal permeation study, study of Blood-brain distribution, TEM (Transmission Electron Microscopy), and CLSM (Confocal Scanning Laser Microscopy) were conducted withal. The NRN-TNopt exhibited spherical as well as sealed vesicles with a considerable small size of 151.3 nm, an EE of 75.23 percent, a PDI of 0.1257, and an in vitro release of 83.32 percent. CLSM investigation revealed that the new formulation allows for higher NRN permeation across nasal mucosa than the NRN solution. The blood-brain distribution investigation revealed that intranasally administered NRN-TN had a greater Cmax and AUC0-24 h than orally administered NRN-TN. Seizure activity and neuromuscular coordination as measured by the rotarod test, biochemical estimate of oxidative stress indicators, and histological investigations demonstrated that the NRN-TN has superior anti-epileptic potential in comparison to the standard diazepam. In addition, nasal toxicity studies demonstrate that the NRN-TN formulation is safer for intranasal administration. This study confirmed that the created TN vesicle formulation is a valuable carrier for the intranasal administration of NRN for the treatment of epilepsy.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"60-76"},"PeriodicalIF":4.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9499026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of multivesicular liposomes for the loco-regional delivery of Vancomycin hydrochloride using active loading method: drug release and antimicrobial properties. 利用活性负载法制备用于局部区域递送盐酸万古霉素的多囊脂质体：药物释放和抗菌特性。

IF 4.4 4区医学

Journal of Liposome Research Pub Date : 2024-03-01 Epub Date: 2023-06-07 DOI: 10.1080/08982104.2023.2220805

Melody Vatankhah, Simin Dadashzadeh, Arash Mahboubi, Azadeh Haeri, Kimia Jandaghi Alaee, Seyed Baubak Mostafavi Naeini, Zahra Abbasian

{"title":"Preparation of multivesicular liposomes for the loco-regional delivery of Vancomycin hydrochloride using active loading method: drug release and antimicrobial properties.","authors":"Melody Vatankhah, Simin Dadashzadeh, Arash Mahboubi, Azadeh Haeri, Kimia Jandaghi Alaee, Seyed Baubak Mostafavi Naeini, Zahra Abbasian","doi":"10.1080/08982104.2023.2220805","DOIUrl":"10.1080/08982104.2023.2220805","url":null,"abstract":"Over the last few years, among controlled-release delivery systems, multivesicular liposomes (MVLs) have attracted attention due to their unique benefits as a loco-regional drug delivery system. Considering the clinical limitations of the current treatment strategies for osteomyelitis, MVLs can be a suitable carrier for the local delivery of effective antibiotics. This study aimed to prepare vancomycin hydrochloride (VAN HL) loaded MVLs using the active loading method which to the best of our knowledge has not been previously reported. Empty MVLS were prepared by the double emulsion (w/o/w) method and VAN HL was loaded into the prepared liposomes by the ammonium gradient method. After full characterization, the release profile of VAN HL from MVLs was assessed at two different pH values (5.5 and 7.4), and compared with the release profile of the free drug and also passively loaded MVLs. In vitro antimicrobial activities were evaluated using the disc diffusion method. Our results demonstrated that the encapsulation efficiency was higher than 90% in the optimum actively loaded MVL. The free VAN HL was released within 6-8 h, while the passively loaded MVLs and the optimum actively loaded MVL formulation released the drug in 6 days and up to 19 days, respectively. The released drug showed effective antibacterial activity against osteomyelitis-causing pathogens. In conclusion, the prepared formulation offered the advantages of sustained-release properties, appropriate particle size as well as being composed of biocompatible materials, and thus could be a promising candidate for the loco-regional delivery of VAN HL and the management of osteomyelitis.","PeriodicalId":16286,"journal":{"name":"Journal of Liposome Research","volume":" ","pages":"77-87"},"PeriodicalIF":4.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9646277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0