Journal of lasers in medical sciences最新文献

{"title":"Immune Response in Laser Tattoo Removal: A Systematic Review.","authors":"Arya Tjipta, Hafiz Ramadhan, Rahmi Amelia Lubis","doi":"10.34172/jlms.2023.66","DOIUrl":"10.34172/jlms.2023.66","url":null,"abstract":"<p><p><b>Introduction:</b> The immune response to laser tattoo removal poses a significant challenge in its management, primarily due to its unpredictable nature, which can range from mild hypersensitivity reactions to severe anaphylaxis. Such responses can potentially hinder the effectiveness of laser tattoo removal procedures. Therefore, gaining a comprehensive understanding of the immune response to tattoo removal using laser techniques is of utmost importance to develop more efficient management strategies. This study aims to address this need by analyzing eight carefully selected articles obtained through a thorough literature review. <b>Methods:</b> To explore the immune response associated with laser techniques in tattoo removal, we employed a rigorous research methodology. A thorough literature review was conducted using reputable search engines such as Google Scholar, SagePub, and PubMed to collect relevant articles. Initially, 788 potential articles were identified through this process. Following meticulous scrutiny, only eight articles that met stringent inclusion criteria were selected for our study. This meticulous selection process ensures that the information presented here is derived from high-quality and pertinent research. <b>Results:</b> Based on the analysis of the eight selected articles, our findings illuminate the various immune responses that emerge following tattoo removal using laser techniques. These responses include hypersensitivity reactions, allergic manifestations, and, in certain instances, anaphylaxis. Hypersensitivity reactions typically manifested as erythema, edema, and pruritus, while allergic responses were observed in the form of urticaria. In summary, our study highlights that the immune response to laser tattoo removal primarily elicits hypersensitivity and, in some cases, anaphylaxis reactions. <b>Conclusion:</b> Our study underscores the significance of clinicians being vigilant regarding potential immune responses during laser tattoo removal. It is crucial to closely monitor patients to promptly address any adverse reactions. Further research holds the potential to enhance our understanding, paving the way for improved management strategies that can enhance patient safety and treatment success.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"14 ","pages":"e66"},"PeriodicalIF":1.5,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10843227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Intercellular Dynamics: Ultra-Weak Biophoton Emission as a Novel Indicator of Altered Cell Functions and Disease in Oligospermia Mice.","authors":"Arefeh Aryan, Fakhroddin Aghajanpour, Masoomeh Dashtdar, Fatemeh Hejazi, Maryam Salimi, Azar Afshar, Reza Soltani, Ahad Hasan Seyed Hasani, Abbas Aliaghaei, Hojjat-Allah Abbaszadeh, Hasan Mahmoodi, Leila Zahedi, Mohammad-Amin Abdollahifar, Fatemeh Fadaei Fathabadi","doi":"10.34172/jlms.2023.65","DOIUrl":"10.34172/jlms.2023.65","url":null,"abstract":"<p><p><b>Introduction:</b> Biophoton emission, the spontaneous release of photons from living cells, has emerged as an attractive field of research in the study of biological systems. Scientists have recently discovered that changes in biophoton emission could serve as potential indicators of pathological conditions. This intriguing phenomenon suggests that cells might communicate and interact with each other through the exchange of these faint but significant light signals. Therefore, the present study introduces intercellular relationships with biophoton release to detect normal and abnormal cell functions to further achieve cellular interactions by focusing on cell and cell arrangement in disease conditions. <b>Methods:</b> Twenty male mice were assigned to control and busulfan groups. Five weeks after the injection of busulfan, the testis was removed, and then the stereological techniques and TUNEL assay were applied to estimate the histopathology of the testis tissue sections. <b>Results:</b> The findings revealed that the ultra-weak biophoton emission in the control group was significantly lower than in the busulfan group. The oligospermia mice model showed that it significantly changed the spatial arrangement of testicular cells and notably decreased the testis volume, length of seminiferous tubules, and the number of testicular cells. The results of the TUNEL assay showed that the percentage of apoptotic cells significantly increased in the busulfan group. <b>Conclusion:</b> The ultra-weak biophoton emission from testis tissue was reduced in oligospermia mice. As a result, the decline of ultra-weak biophoton can indicate a change in cell arrangement, a decrease in intercellular interaction, and eventually disease.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"14 ","pages":"e65"},"PeriodicalIF":1.5,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10843233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Doppler Finding, Cardiovascular Function Assessment, and Fetuses' Survival Following the Fetoscopic Laser in Twin-to-Twin Transfusion Syndrome.","authors":"Tahmineh Ezazi Bojnordi, Laleh Eslamian, Vajiheh Marsoosi, Alireza Golbabaei, Mehrdad Sheikh Vatan, Alireza A Shamshirsaz, Nasim Eshraghi, Marjan Ghaemi","doi":"10.34172/jlms.2023.64","DOIUrl":"10.34172/jlms.2023.64","url":null,"abstract":"<p><p><b>Introduction:</b> This study aimed to evaluate the effectiveness of selective laser photocoagulation of communicating vessels (SLPCV) on cardiac function in twins with twin-to-twin transfusion syndrome (TTTS). <b>Methods:</b> This retrospective cohort study evaluated 178 women with twin pregnancies complicated with TTTS and scheduled for SLPCV between 16 and 26 weeks of gestation. The severity of TTTS was determined by Quintero staging and the severity of cardiovascular disorders by the CHOP (Children's Hospital of Philadelphia) score. Patient survival was evaluated through a one-month-after-birth follow-up of fetuses. <b>Results:</b> The study revealed significant improvements in Doppler indices in both donors and recipients after SLPCV. The CHOP score also significantly decreased after the intervention. One-month-after-birth survival rates were 55.1% in donors and 56.7% in recipients. Some Doppler indexes of fetuses before SLPCV could predict survival until one month after birth. <b>Conclusion:</b> The study suggests that SLPCV can improve cardiac function in fetuses with TTTS and that some Doppler indexes can predict survival outcomes. Additionally, the severity of TTTS can be a powerful indicator of the severity of cardiovascular complications.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"14 ","pages":"e64"},"PeriodicalIF":1.5,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10843211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invariant point message passing for protein side chain packing.","authors":"Nicholas Z Randolph, Brian Kuhlman","doi":"10.1101/2023.08.03.551328","DOIUrl":"10.1101/2023.08.03.551328","url":null,"abstract":"<p><p>Protein side chain packing (PSCP) is a fundamental problem in the field of protein engineering, as high-confidence and low-energy conformations of amino acid side chains are crucial for understanding (and designing) protein folding, protein-protein interactions, and protein-ligand interactions. Traditional PSCP methods (such as the Rosetta Packer) often rely on a library of discrete side chain conformations, or rotamers, and a forcefield to guide the structure to low-energy conformations. Recently, deep learning (DL) based methods (such as DLPacker, AttnPacker, and DiffPack) have demonstrated state-of-the-art predictions and speed in the PSCP task. Building off the success of geometric graph neural networks for protein modeling, we present the Protein Invariant Point Packer (PIPPack) which effectively processes local structural and sequence information to produce realistic, idealized side chain coordinates using <i>χ</i>-angle distribution predictions and geometry-aware invariant point message passing (IPMP). On a test set of ~1,400 high-quality protein chains, PIPPack is highly competitive with other state-of-the-art PSCP methods in rotamer recovery and per-residue RMSD but is significantly faster.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"6 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10769188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90050848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the Diode Laser Wavelengths 445 nm and 810 nm in Gingival Depigmentation – A Clinical Evaluation","authors":"Seyed Masoud Mojahedi Nasab, Matthias Frentzen, Andreas Mayr, Somayeh Rahmani, F. Anbari, J. Meister, Saranaz Azari-Marhabi, Deniz Mojahedi Nasab","doi":"10.34172/jlms.2023.63","DOIUrl":"https://doi.org/10.34172/jlms.2023.63","url":null,"abstract":"Introduction: Nowadays, esthetic appearance plays an important role in the field of dentistry. Discolorations and pigmentations of the gingiva reduce the appearance of a healthy-looking smile. On this occasion, the use of lasers shows a promising approach for a fast and non-aggressive treatment in this field. Different laser wavelengths are being used for gingival depigmentation this clinical study aimed to investigate the effect of the novel laser wavelength (445 nm) in this field and compare it with an 810 nm diode laser. Methods: Two diode laser wavelengths (445 nm and 810 nm) were used for depigmentation. The laser output power chosen was 1 W. An optical fiber with a diameter of 400 µm was used. 21 patients with pigmented gingiva were selected. Depigmentation was carried out in a split-mouth design for a direct comparison of the clinical effect. Outcomes were documented by photograph after one month and six months of follow-up. Results: For each wavelength, 21 volunteers evaluated 21 clinical cases of depigmentation, which means that 441 comparisons were carried out in total regarding the color change from brownish to pinkish. A 100% clarification was achieved for 445 nm. In the 810 nm group, the color change in 44 of 441 cases (10%) could not be clearly identified. No statistically significant difference in pain experience was reported for both laser treatments. Conclusion: The clinical evaluation showed that within the limitations of this study, most of the clinical outcome parameters were highly acceptable by the patients due to mild pain and discomfort for both laser systems.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"525 ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139171904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Effect of Phosphoric Acid or Er: YAG Laser on the Shear Bond Strength of Orthodontic Brackets to Enamel Surfaces Followed by 980-Laser Assisted Bleaching: An In Vitro Study","authors":"Mohammad Oladzad, N. Chiniforush, R. Bahrami, A. Mirhashemi","doi":"10.34172/jlms.2023.62","DOIUrl":"https://doi.org/10.34172/jlms.2023.62","url":null,"abstract":"Introduction: The residual oxygen remained on tooth surface after bleaching may interfere with adhesion of brackets to enamel surface. This study aimed to evaluate the influence of phosphoric acid and Er: YAG laser as an etching technique on the shear bond strength (SBS) of orthodontic brackets to enamel surfaces after 980-laser-assisted bleaching. Method: A total of 84 human premolars were recruited in the present study. Samples were divided into 6 groups including conventional bleaching with conventional etching, conventional bleaching with Er: YAG laser etching, laser-assisted bleaching with conventional etching, and Laser-assisted bleaching with Er: YAG laser etching, without bleaching with traditional etching, and without bleaching+Er: YAG laser etching. Following thermocycling, the debonding of brackets was conducted using a universal testing machine. Scanning electron microscope (SEM) microscope evaluation and adhesive remnant index (ARI) scores were assessed. The comparison of SBS values between groups was carried out by means of a one-way analysis of variance, followed by post-hoc tests. Results: The non-bleaching with conventional etching group showed the highest SBS mean value (23.45±5.16 MPa), whereas the conventional bleaching with conventional etching group represented the lowest SBS mean value (8.8±3.83 MPa). In all groups, the most common type of failure was classified as either score 1 or score 2. No significant difference was observed in terms of SBS mean between the groups (P=0.165); however, the average SBS of bleached teeth was significantly lower, compared to the non-bleached group (P=0.000). Honeycomb structure and porosity were observed following Er: YAG laser etching on the tooth surface. Conclusion: Increased bond strength of brackets was observed in bleached teeth following Er: YAG laser etching. Therefore, if necessary, bond the brackets on the same day of bleaching will be done, the application of Er:YAG laser as etching technique will can be recommended.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"223 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139181253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histologic Evaluation of Effect of Three Wavelengths of Diode Laser on Human Gingival Margins","authors":"Pilar Rocío Blanco Flórez, Helida Helena Avendaño Maz, Josep Arnabat Domínguez, Antonio Jesús España Tost, Jennifer Orozco Páez","doi":"10.34172/jlms.2023.61","DOIUrl":"https://doi.org/10.34172/jlms.2023.61","url":null,"abstract":"Introduction: Available evidence suggests that the response of the intervened tissue is directly linked to the effects generated by the cutting instrument used. To determine the histological findings in gingival tissue margins excised through gingivectomies performed using 450 nm, 940 nm, and 980 nm diode lasers. The present study aimed to determine the histological findings in gingival tissue margins excised through gingivectomies performed using 450 nm, 940 nm, and 980 nm diode lasers. Methods: Gingival tissue samples were collected from 30 patients who had undergone gingivectomy procedures. Each study group comprised 10 patients who willingly provided their samples after providing informed consent. The visualization of histological findings was facilitated through Hematoxylin-Eosin staining. Additionally, variables related to pain and hemostasis were assessed during the intraoperative period. Results: The incision quality was categorized as irregular across all three wavelengths. Histological examination of the epithelial tissue revealed the absence of carbonization and the preservation of cell morphology in over 50% of the resection margin in samples obtained with the 450 nm and 940 nm wavelengths. In the connective tissue, observations included carbonization, collagen coagulation, and basophilia, with the 980 nm wavelength demonstrating the highest percentage of samples displaying collagen coagulation in more than 50% of the resection margin. Conversely, the 450 nm wavelength exhibited the highest degree of preservation of the fibroblast structure. Conclusion: Based on a comprehensive analysis of the study results, it can be inferred that the 450nm and 940nm wavelength lasers tend to produce less thermal damage and better cell preservation when compared to the 980nm wavelength.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"62 5","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139183708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy Evaluation of Human Skin Treatment with Photodynamic Therapy in Actinic Keratoses Patients.","authors":"Zahra Razzaghi, Babak Arjmand, Maryam Hamzeloo-Moghadam, Mostafa Rezaei Tavirani, Mona Zamanian Azodi","doi":"10.34172/jlms.2023.60","DOIUrl":"https://doi.org/10.34172/jlms.2023.60","url":null,"abstract":"<p><p><b>Introduction:</b> Photodynamic therapy (PDT) is a combined method of light and light-activated chemicals that are called photosensitizers (PSs). PDT is recommended as a high cure rate method with fewer side effects and a noninvasive tool to treat cancer. This study aimed to evaluate PDT efficacy as a therapeutic method against actinic keratoses in patients via protein-protein interaction (PPI) network analysis by using the gene expression profiles of Gene Expression Omnibus (GEO). <b>Methods:</b> Twenty-one gene expression profiles were extracted from GEO and analyzed by GEO2R to determine the significant differentially expressed genes (DEGs). The significant DEGs were included in PPI networks via Cytoscape software. The networks were analyzed by the \"Network Analyzer\", and the elements of the main connected components were assessed. <b>Results:</b> There were three main connected components for the compared sets of the gene expression profiles including the lesional region of skin before (Before set) and after (After set) PDT versus healthy (healthy set) skin and before versus after. The before-health comparison showed a partial similarity with the After-Healthy assessment. The before-after evaluation indicated that there were not considerable differences between the gene expression profile of the lesional region before and after PDT. <b>Conclusion:</b> In conclusion, PDT was unable to return the gene expression pattern of the actinic keratoses skin to a healthy condition completely.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"14 ","pages":"e60"},"PeriodicalIF":1.5,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10746884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139032406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the Response of Human Umbilical Vein Endothelial Cells to Photodynamic Therapy: Highlighting the Role of Il-17 Signaling Pathway.","authors":"Mona Zamanian Azodi, Babak Arjmand, Maryam Hamzeloo-Moghadam, Mostafa Rezaei Tavirani, Zahra Razzaghi, Alireza Ahmadzadeh, Reza M Robati, Majid Rezaei Tavirani","doi":"10.34172/jlms.2023.59","DOIUrl":"https://doi.org/10.34172/jlms.2023.59","url":null,"abstract":"<p><p><b>Introduction:</b> Photodynamic therapy (PDT) is a method based on the application of a photosensitive agent and the administration of light irradiation on the treated samples. PDT is applied as an effective tool with minimal side effects against tumor tissues. This study aimed to assess the targets of critical genes by PDT at the cellular level of cancer to provide a new perspective on its molecular mechanism. <b>Methods:</b> To assess the effect of PDT, we extracted the differentially expressed genes (DEGs) from the gene expression profiles of human umbilical vein endothelial cells (HUVECs) treated with PDT from Gene Expression Omnibus (GEO) databases. The queried DEGs were evaluated via a regulatory network and gene ontology enrichment to find the critical targets. <b>Results:</b> Among 76 queried significant DEGs, 27 individuals were interacted by activation, inhibition, and co-expression actions. Thirty DEGs were related to the five classes of biological terms. The IL-17 signaling pathway and PTGS2, CXCL8, FOS, JUN, CXCL1, ZFP36, and FOSB were identified as the crucial targets of PDT. <b>Conclusion:</b> PDT as a stimulator of gene expression and an activator of gene activity overexpressed and hyper-activated many genes. It seems that PDT introduces a number of genes and pathways that can be regulated by anticancer drugs to fight against cancers.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"14 ","pages":"e59"},"PeriodicalIF":1.5,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10746882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139032404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Low-Level Laser Irradiation (810 nm) on the Proliferation and Differentiation of Osteoblast-Like Cells Cultured on SLA Titanium Discs Exposed to a Peri-implantitis Environment.","authors":"Evangelia P Zampa, Kyriaki Kyriakidou, Joseph Papaparaskevas, Eudoxie Pepelassi, Ioannis K Karoussis","doi":"10.34172/jlms.2023.57","DOIUrl":"https://doi.org/10.34172/jlms.2023.57","url":null,"abstract":"<p><p><b>Introduction:</b> Elimination of inflammation and re-osseointegration are the major objectives of peri-implantitis therapy. Existing data, however, do not support any decontamination approach. Thus, the present <i>in vitro</i> study aims to assess whether the air-debriding decontamination method with erythritol powder restores the biocompatibility of infected titanium discs and to investigate the potent biomodulatory ability of diode laser (810 nm) irradiation to promote cell proliferation and differentiation of premature osteoblast-like cells (MG63) towards osteocytes. <b>Methods:</b> The experimental groups consisted of cells seeded on titanium discs exposed or not in a peri-implantitis environment with or without biomodulation. Infected discs were cleaned with airflow with erythritol powder. Cell cultures seeded on tricalcium phosphate (TCP) surfaces with or without biomodulation with a laser (810 nm) were used as controls. The study evaluated cell viability, proliferation, adhesion (SEM) at 24, 48 and 72 hours, and surface roughness changes (profilometry), as well as the effects of low-level laser therapy (LLLT) on ALP, OSC, TGF-b1, Runx2, and BMP-7 expression in MG63 cells' genetic profile on days 7, 14, and 21. <b>Results:</b> The MTT assay as well as the FDA/PI method revealed that cell proliferation did not show significant differences between sterile and decontaminated discs at any timepoint. SEM photographs on day 7 showed that osteoblast-like cells adhered to both sterile and disinfected surfaces, while surface roughness did not change based on amplitude parameters. The combination of airflow and LLLT revealed a biomodulated effect on the differentiation of osteoblast-like cells with regard to the impact of laser irradiation on the genetic profile of the MG63 cells. <b>Conclusion:</b> In all groups tested, osteoblast-like cells were able to colonize, proliferate, and differentiate, suggesting a restoration of biocompatibility of infected discs using airflow. Furthermore, photomodulation may promote the differentiation of osteoblast-like cells cultured on both sterile and disinfected titanium surfaces.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"14 ","pages":"e57"},"PeriodicalIF":1.5,"publicationDate":"2023-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10746885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139032405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}