Journal of lasers in medical sciences最新文献

{"title":"Evaluation of the Cellular Resistance Process in Treated Cells Via Extracorporeal Photopheresis","authors":"Babak Arjmand, Mostafa Rezaei Tavirani, Somayeh Jahani Sherafat, Mitra Rezaei, Masoumeh Farahani, Majid Rezaei Tavirani","doi":"10.34172/jlms.2023.46","DOIUrl":"https://doi.org/10.34172/jlms.2023.46","url":null,"abstract":"Introduction: Extracorporeal photopheresis (ECP) is a therapeutic method applied against some diseases such as cancers. Using 8-methoxypsoralen (8-MOP) and UVA radiation in ECP is associated with achievement in the treatment of patients with leukemic cutaneous T-cell lymphoma (CTCL). Evaluation of cellular resistance versus ECP is the aim of this study. Methods: Data were downloaded from the Gene Expression Omnibus (GEO) database and were analyzed via the GEO2R program. The significant DEGs were assessed via protein-protein interaction (PPI) network analysis by using the STRING database and Cytoscape software. The critical genes were evaluated via gene ontology by using the ClueGO application of Cytoscape software. The identified biological processes were determined and analyzed. Results: Fifty-seven significant DEGs were determined. The main connected component of the PPI network including 32 queried significant DEGs plus 50 first neighbors was constructed. Nineteen histones as critical nodes were assessed via gene ontology, and \"nucleosome organization\" was pointed out as the crucial biological process. Finally, 15 histones from H2A, H2B, and H3 histone families were identified as the key genes that are involved in the resistance property of the treated cells. Conclusion: In conclusion, 15 members of H2A, H2B, and H3 families (especially H2A family) were considered as the origin of resistance versus ECP treatment. It is concluded that sensitivity to ECP treatment depends on gross molecular events which are involved in the functions of histones.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Low-Level Laser Therapy on Differentiation and Proliferation of Human Dental Pulp Stem Cells: A Systematic Review","authors":"Hamed Karkehabadi, Jaber Zafari, Elham Khoshbin, Roshanak Abbasi, Sogand Esmailnasab, Amin Doosti-Irani","doi":"10.34172/jlms.2023.47","DOIUrl":"https://doi.org/10.34172/jlms.2023.47","url":null,"abstract":"Introduction: Considering the positive impact of laser treatment on the proliferation of certain cell types, we opted to perform a systematic review aimed at evaluating the effects of laser therapy and photobiomodulation on the proliferation and differentiation of human dental pulp stem cells (hDPSCs). Methods: We included all research studies examining the impact of laser therapy on hDPSCs, without limitations on publication dates or article languages. The major international databases, including PubMed, ISI Web of Science, and Scopus, were searched from inception to April 2022 by the relevant keywords. Results: In total, 1886 studies were identified in the initial search from the mentioned databases and other sources. Finally, 17 relevant studies were included in the present systematic review after removing duplicates and non-relevant articles. The results indicated the useful effect of low-level laser therapy (LLLT) on the hDPSCs. Conclusion: The findings of this systematic review indicate the useful role of LLLT in cell therapy, proliferation, and differentiation associated with hDPSCs.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136078472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory, Antioxidant, and Wound-Healing Effects of Photobiomodulation on Type-2 Diabetic Rats","authors":"Atefeh Moheghi, Seyyed Mohammad Hossein Noori Mougehi, Abdollah Amini, Atarodalsadat Mostafavinia, Fatemehalsadat Rezaei, Fatemeh Bagheri Tadi, Sufan Chien, Mohammad Bayat","doi":"10.34172/jlms.2023.45","DOIUrl":"https://doi.org/10.34172/jlms.2023.45","url":null,"abstract":"Introduction: In the current study, the effects of photobiomodulation (PBM) treatments were examined based on biomechanical and histological criteria and mRNA levels of catalase (CAT), superoxide dismutase (SOD), and NADPH oxidase (NOX) 1 and 4 in a postponed, ischemic, and infected wound repair model (DIIWHM) in rats with type 2 diabetes (DM2) during the inflammation (day 4) and proliferation (day 8) stages. Methods: To study ischemic wound repair in a diabetic rat model (DIIWHM), 24 rats with type-2 diabetes were randomly divided into four groups and infected with methicillin-resistant Staphylococcus aureus (MRSA). The control groups consisted of CG4 (control group on day 4) and CG8 (control group on day 8), while the PBM groups comprised PBM4 (PBM treatment group on day 4) and PBM8 (PBM treatment group on day 8). These group assignments allowed for comparisons between the control groups and the PBM-treated groups at their respective time points during the study. Results: On days 4 and 8 of wound restoration, the PBM4 and PBM8 groups showed substantially modulated inflammatory responses and improved formation of fibroblast tissue compared with the CG groups (P<0.05). Concurrently, the effects of PBM8 were significantly superior to those of PBM4 (P<0.05). The antioxidant results on days 4 and 8 revealed substantial increases in CAT and SOD in the PBM groups compared with the CGs (P<0.05). Substantial decreases were observed in the antioxidant agents NOX1 and NOX4 of the PBM4 and PBM8 groups compared with both CGgroups (P<0.05). Conclusion: PBM treatments significantly sped up the inflammatory and proliferating processes in a DHIIWM in DM2 animals by modifying the inflammatory reaction and boosting fibroblast proliferation. Overall, the current findings indicated substantially better results in the PBM groups than in the CG groups.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Effectiveness of the Carbon Dioxide (CO2) Laser in Minor Oral Surgery: A Systematic Review","authors":"Mohammad Asnaashari, Mohammadreza Behnam Roudsari, Maryam Sadat Shirmardi","doi":"10.34172/jlms.2023.44","DOIUrl":"https://doi.org/10.34172/jlms.2023.44","url":null,"abstract":"Introduction: Lasers in oral surgery have been extensively studied in recent years. Laser treatment is now a well-known technology that is frequently employed on oral soft tissues. The carbon dioxide (CO2) laser was one of the first soft tissue removal lasers. Because of the strong affinity of the CO2 laser to water, it is best used for removing, vaporizing, and coagulating these tissues. In minor oral surgery, CO2 laser therapy has shown advantages. Therefore, this study examined the CO2 laser use in minor oral soft tissue surgery. Methods: The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) standards were followed in this study. A question for research encompassing the inclusion criteria for the participants, intervention, comparison, outcome, and study design (PICOS) was formulated. The search queries were entered into the PubMed/Medline, Scopus, and Embase databases. Consideration was given to publications published between January 1, 2018 and March 15, 2023. Results: The research included 37 studies after narrowing search results, eliminating duplicate titles, and conducting an eligibility review (three animal studies, seven case reports, three case series, and twenty-four clinical studies). CO2 lasers alone or in combination with other therapies successfully treated oral potentially malignant disorders (OPMDs), oral tumors, oral fibrous-epithelial lesions, gingival melanin hyperpigmentation, pyogenic granuloma, socket preservation, mucocele, high labial frenulum attachment, and so on. CO2 lasers reduced intra- and postoperative complications and adverse effects, improved postoperative functional results, ablated tissues with precision, and minimized disease recurrence and malignant transformation. Conclusion: Our study found that the CO2 laser in oral minor surgeries is successful, but further randomized clinical trials and multicenter studies are recommended to compare CO2 laser surgery to other treatments.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Erbium:YAG Laser Recycling on Mechanical Characteristics of Retrieved Orthodontic Mini-screws","authors":"Soghra Yassaei, Hossien Agha Aghili, Atie Behrouzirad","doi":"10.34172/jlms.2023.42","DOIUrl":"https://doi.org/10.34172/jlms.2023.42","url":null,"abstract":"Introduction: This study aimed to evaluate the influence of two recycling methods on the mechanical and surface characteristics of orthodontic mini-screws. Methods: Thirty-six retrieved mini-screws were randomly classified into two equal groups. In the first group (laser recycled group (LG)), the Er:YAG laser (2940 nm, 5.5 W, 275 mJ, perpendicular to the mini-screws at a distance of 7-10 mm, 25 s) was used to recycle mini-screws. In the second group (phosphoric acid and sodium hypochlorite recycled group (ASG)), the mini-screws were kept in 37% phosphoric acid gel (10 minutes) and then placed in 5.25% sodium hypochlorite for 30 minutes. Eighteen new mini-screws were selected as the control group (CG). Maximum insertion torque (MIT), maximum removal torque (MRT), and fracture torque (FT) of all mini-screws were measured. A sample from each group was examined for the surface changes of the mini-screw and tissue remnants under a scanning electron microscope (SEM). Results: The mean MIT was significantly higher in both LG and ASG groups than the CG (P<0.001 and P=0.002, respectively). However, no significant difference was shown between the LG and ASG groups. The mean values of MRT and FT showed no significant difference between the groups. The amount of tissue remnants in the ASG group was significantly higher than that in the LG group. The evidence of porosity and corrosion was observed on the ASG mini-screw surface, and there was an increase in roughness on the LG mini-screw surface. Conclusion: The Er:YAG laser recycling of mini-screws is a proper method causing minimum damage to the screw surface.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136252520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Effects of Er:YAG Laser, Sodium Hypochlorite, and QMix on Root Canals Infected With <i>Enterococcus faecalis</i> and <i>Candida albicans</i>","authors":"Agime Dragidella, Ariana Kameri","doi":"10.34172/jlms.2023.43","DOIUrl":"https://doi.org/10.34172/jlms.2023.43","url":null,"abstract":"Introduction: During endodontic treatment, the smear layer can reduce disinfectant efficacy. The aim of this study was to evaluate the antimicrobial efficacy of Er:YAG laser radiation and its combination with NaOCl and QMix against Enterococcus faecalis and Candida albicans and their comparison with conventional irrigation with only NaOCl and QMix. Methods: Two hundred extracted single-rooted teeth after root canal preparation were divided into two groups and inoculated with E. faecalis and C. albicans. According to the treatment method, all samples were divided into five treatment groups: (1) Er:YAG laser, (2) NaOCl, (3) QMix, (4) Er:YAG laser plus NaOCl, and (5) Er:YAG laser plus QMix. After 24 hours of agar plate cultivation, cell viability was recorded with a flow cytometer. Results: All treatment modalities showed efficiency in the reduction of microbial cells. For laser treatment alone after exposure for 90 seconds, significantly fewer non-death cells were seen, compared to 30-second treatment. For both timings (30 and 90 seconds), irrigation with NaOCl or QMix after laser application resulted in significantly fewer vital cells of E. faecalis and C. albicans, compared with laser treatment alone (P<0.001). The samples treated with only NaOCl showed a significantly higher percentage of vital E. faecalis and Candida albicans cells, compared to the samples treated with only QMix (P<0.001). Conclusion: Higher Er: YAG laser exposure time (90 seconds) after its combination with QMix and NaOCl improves the efficacy in the reduction of E. faecalis and C. albicans from the root canal, compared to 30-second laser exposure time and conventional irrigation methods with NaOCl and QMix.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"2014 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136252517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Photobiomodulation on the Treatment of Hereditary Mitochondrial Diseases","authors":"Richard Baskerville, Nykle Krijgsveld, Patrick Esser, Glen Jeffery, Joanna Poulton","doi":"10.34172/jlms.2023.41","DOIUrl":"https://doi.org/10.34172/jlms.2023.41","url":null,"abstract":"Introduction: Despite a wide variety of clinical presentations in hereditary Mitochondrial Diseases, muscle fatigue is a common theme and impairs a patient’s quality of life and ability to function. Current treatments are only supportive and include nutritional supplementation and physical therapy. Photobiomodulation therapy (PBMT) using low-intensity, narrow spectrum light in the red/near infrared (NIR) range, from a low-level laser or light-emitting diode sources, enhances mitochondrial function in preclinical and clinical studies on a range of conditions. However, little research has been done on the effectiveness of photobiomodulation in hereditary mitochondrial disorders. Methods: We performed a scoping review of the evidence of the beneficial effects of photobiomodulation for treating the muscle-related symptoms of hereditary mitochondrial disease. Results: No studies regarding photobiomodulation in hereditary mitochondrial disease were identified. However, in other clinical conditions featuring acquired mitochondrial impairment, we identified studies that suggested improved function, although sample sizes were small in number and statistical power. Conclusion: There is emerging evidence of efficacy for PBMT for diseases involving acquired mitochondrial insufficiency. We identified no published research on PBMT in hereditary mitochondrial disease, but this review confirms a theoretical rationale for a positive effect and suggests further research.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136360072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Radiosensitivity: Effects of Acute Ionizing Radiation on Inflammation and Apoptosis in Macrophage Cell Line (RAW 264.7)","authors":"Zahra Bagheri-Hosseinabadi, Jaber Zafari, Fatemeh Javani Jouni, Hanieh Sadeghi, Mitra Abbasifard","doi":"10.34172/jlms.2023.40","DOIUrl":"https://doi.org/10.34172/jlms.2023.40","url":null,"abstract":"Introduction: The responses of biological systems to various types of radiation have multifaceted dimensions. In the field of ionizing radiation, in vitro external gamma radiation therapy has primarily been studied as a model to elucidate the challenges that biological systems face from radiation effects. Exposure of cells/organisms to gamma radiation results in a cascade of ionization events that can cause severe and irreversible biological damage. However, the biological responses and oxidative stress-related mechanisms under acute radiation conditions remain poorly understood in inflammatory systems. The present study aimed to provide a model of the effect of ionizing radiation on macrophages, which play a pivotal role in the mechanisms of inflammation, to assess the impact of radiotherapy as an approach to treating inflammatory diseases. Methods: A macrophage cell line (RAW 264.7) was cultured and exposed to different doses of gamma radiation (4, 6, 8, 10 Gy). Cell viability, apoptosis, cell cycle, migration, nitric oxide (NO) and prostaglandin E2 (PGE2) production, expression of pro-inflammatory and apoptotic genes, and cytokine secretion of macrophages were also evaluated. Results: The results showed that gamma radiation at 4 Gy had a low effect on macrophage characteristics and cytokine secretion patterns. In contrast, higher doses (8 and 10 Gy) increased DNA damage, expression of apoptotic genes, and secretion of NO and PGE2 cytokines. 6 Gy radiation, the maximum radiation dose, showed moderate non-destructive effects and inflammation process modulation. In this study, doses higher than 6 Gy of Gamma radiation caused cell mortality. Conclusion: It appears that 6 Gy of gamma radiation modulates the inflammatory cascade caused by macrophage cells.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135149204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Capillary Hemangioma Managed With Laser Ablation: A Case Report","authors":"Aravind Warrier S, Vaishnavi Muthukumaran, Aparna Venkatramakrishnan, Divyambika CV, Thamizhchelvan H, Muthukumar Santhanakrishnan","doi":"10.34172/jlms.2023.39","DOIUrl":"https://doi.org/10.34172/jlms.2023.39","url":null,"abstract":"Introduction: Hemangiomas are hamartomatous benign tumours of the vascular tissue, which are common among infants and adolescents and are found in the head and neck region. Oral hemangiomas are infrequently encountered in the palate. Baseline soft tissue imaging must be considered for an appropriate diagnosis and treatment approach. Case Report: A 68-year-old female presented with a painless growth in the roof of her mouth that gradually increased in its size. She was subjected to intraoral ultrasonography and was diagnosed with capillary hemangioma. In this instance, laser ablation was chosen as the preferred treatment due to the complications associated with the conventional surgery. Conclusion: This case report enlightens the systematic diagnostic approach and treatment plan.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"96 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135081970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Er,Cr:YSGG Laser, Sandblast, and Acid Etching Surface Modification on Surface Topography of Biodental Titanium Implants","authors":"Mohammed A Abdulla, Radhwan H Hasan, Osama Hazim Al-Hyani","doi":"10.34172/jlms.2023.38","DOIUrl":"https://doi.org/10.34172/jlms.2023.38","url":null,"abstract":"Introduction: Several techniques have been used to modify the surface of commercially pure titanium (CPTi) implants to improve osseointegration using lasers, sandblasts, sandblasts with acid etching, and other modalities. For implant-osseointegration, surface features like chemical composition of a surface, topography, and surface energy are essential. The present comparative study aimed to compare the impact of Er,Cr:YSGG laser, sandblasting, and acid etching implant surface modifications on the surface topography, roughness, and element chemical composition of the Ti dental implant. Methods: Thirty CPTi dental implants were divided into three groups according to the surface modification (n=10 for each group): Group A: Sandblasting with acid etching (SLActive), group B: Sandblasting, and group C: Er,Cr:YSGG laser surface modifications. The modified surfaces were analyzed using scanning electron microscopy (SEM), profilometer, and energy dispersive x-ray spectrometry (EDS). Results: One-way analysis of variance (ANOVA) showed that there were significant differences in the mean values of average roughness (Ra) of the three groups (P<0.05). Tukey’s post hoc test showed that the average roughness (Ra) of laser-surface modification (group C) of the implant had the highest mean value (2.30 µm) among the different groups, while sandblasted surface modification (group B) of the implant had the lowest mean value (1.39 µm). The SLActive (group A) sandblast with acid etching had a mean value of 1.63 µm. SEM analysis showed that significantly modified surface topographies and different element concentrations were found within all modified groups. Conclusion: The Er,Cr:YSGG laser irradiation increased the implant surface roughness value after implant surface modification, compared to sandblasts and sandblasts with acid etching application. The observations for the SEM-EDS analysis revealed several elements with different concentrations, which were affected by the physical–chemical characteristics of the surface modification techniques. The SEM analysis showed a significant modification in implant surface topographies of the tested groups.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135081633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}