Journal of lasers in medical sciences最新文献

{"title":"Effect of Non-thermal Plasma Therapy on Pushout Bond Strength of Epoxy Resin and Tricalcium Silicate-Based Endodontic Sealers.","authors":"Saeede Zadsirjan, Mohammad Asnaashari, Tina Estarami, Soolmaz Heidari, Babak Shokri, Atoosa Yazdani","doi":"10.34172/jlms.2023.70","DOIUrl":"10.34172/jlms.2023.70","url":null,"abstract":"<p><p><b>Introduction:</b> The current study aimed to assess the effect of non-thermal plasma (NTP) on the pushout bond strength (PBS) of epoxy resin and tricalcium silicate-based endodontic sealers. <b>Methods:</b> Forty single-canal extracted teeth were decoronated at the coronal region, underwent root canal preparation, and were assigned to four groups (n=10) for the application of AH26 sealer, NTP+AH26 (P-AH26), Endoseal TCS sealer, and NTP+Endoseal TCS sealer (P-TCS). The root canals were sectioned into 1 mm slices, and the PBS value was measured in a universal testing machine. Data were analyzed by the Friedman, Kruskal-Wallis, and Dunn tests (<i>P</i><0.05). <b>Results:</b> The PBS of TCS and P-TCS groups was not significantly different (<i>P</i>>0.05). The PBS of the P-AH26 group was significantly lower than that of the AH26 group in the middle third (<i>P</i><0.05). The PBS of the AH26 group was higher than the other groups in all sections. The PBS in the apical third was lower than other sections in all groups (<i>P</i><0.05). <b>Conclusion:</b> NTP had no significant effect on the PBS of Endoseal TCS. NTP significantly decreased the PBS of AH26 sealer in the middle third but had no significant effect on its bond strength in other sections.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10843224/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blue Laser-Activated Silver Nanoparticles from Grape Seed Extract for Photodynamic Antimicrobial Therapy Against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>.","authors":"Ahmad Khalil Yaqubi, Suryani Dyah Astuti, Andi Hamim Zaidan, Dezy Zahrotul Istiqomah Nurdin","doi":"10.34172/jlms.2023.69","DOIUrl":"10.34172/jlms.2023.69","url":null,"abstract":"<p><p><b>Introduction:</b> Living organisms, particularly humans, frequently encounter microorganisms like bacteria, fungi, and viruses in their surroundings. Silver nanoparticles are widely used in biomedical devices due to their antibacterial, antifungal, and antiviral properties. The study evaluates the efficacy of blue laser and silver nanoparticles from grape seed extract (AgNPs-GSE) in reducing gram-negative <i>Escherichia coli</i> and gram-positive <i>Staphylococcus aureus</i> bacteria causing infections. <b>Methods:</b> The sample consisted of four groups: a control without laser irradiation (T0), <i>E. coli</i> samples (A1 and A2) irradiated with a 405 nm diode laser at different times and concentrations of silver nanoparticles, and <i>S. aureus</i> samples (A3 and A4) irradiated with a 405 nm diode laser at different times and concentrations. Bacteria in groups A2 and A4 were treated with a photosensitizer (PS) made from grape seed extracts, incubated for 10 minutes, and then irradiated for 90, 120, 150, and 180 seconds. The samples were cultured on Tryptic Soy Agar (TSA) media, incubated at 37 °C, counted by using a Quebec colony counter, and analyzed using ANOVA and Tukey tests with a significance level of <i>P</i><0.05. <b>Results:</b> The study found that 10 µl of AgNPs-GSE, when combined with exposure to a blue laser at 405 nm and a dose of 3.44 J/cm², can effectively photoinactivate <i>E. coli</i> and <i>S. aureus</i> bacteria. The addition of AgNPs-GSE to <i>E. coli</i> bacteria led to a significant reduction in their viability, with a reduction of 73.93%, 80.96%, and 83.80%, respectively. Similarly, when <i>S. aureus</i> bacteria were irradiated for 180 seconds by adding 1 mM, 1.5 mM, and 2 mM AgNPs-GSE, bacterial viability was reduced by 70.87%, 78.04%, and 87.01%, respectively. <b>Conclusion:</b> The findings from the present study indicate that at an energy density of 3.44 J/cm², it was possible to inactivate <i>E. coli</i> by 83.80% and <i>S. aureus</i> by 87.01%.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10843232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation Therapy for Managing Motor and Sensory Dysfunctions Following Temporomandibular Joint Surgery: A Case Report.","authors":"Luiz Felipe Palma, Thales Dos Santos Porfírio, Rafael Verardi Serrano, Luana Campos, Luís Otávio Carvalho de Moraes","doi":"10.34172/jlms.2023.67","DOIUrl":"10.34172/jlms.2023.67","url":null,"abstract":"<p><p><b>Introduction:</b> Surgical procedures involving the temporomandibular joint (TMJ) are frequently associated with nerve injuries and subsequent dysfunctions. Considering that traumatic peripheral nerve injuries may resolve slowly and their prognosis is generally unpredictable, the current study aimed to report a clinical case in which both motor (affecting the temporal and zygomatic branches of the facial nerve) and sensory dysfunctions (affecting the auriculotemporal nerve of the trigeminal nerve) following TMJ surgery were effectively treated by using photobiomodulation therapy (PBMT). <b>Case Report:</b> PBMT sessions, involving a total of 30 facial points, were administered twice a week for 10 weeks. The following parameters were utilized: wavelength of 808 nm, energy density of 75 J/cm², power output of 100 mW, total energy of 3 J, and duration of 30 seconds per point. A considerable improvement in both facial asymmetry and muscle function was achieved within 5 weeks, along with a total restoration of cutaneous sensitivity. By the 10th week of PBMT, the facial movement dysfunction was completely resolved. <b>Conclusion:</b> According to the current case, PMBT seems to be an effective intervention to manage motor and sensory nerve dysfunctions following TMJ surgery.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10843219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topographical Analysis of Human Enamel after Phosphoric Acid Etching and Er,Cr:YSGG Laser Irradiation.","authors":"Saja Qasim Salman, Basima Mohammed Ali Hussein","doi":"10.34172/jlms.2023.68","DOIUrl":"10.34172/jlms.2023.68","url":null,"abstract":"<p><p><b>Introduction:</b> Dental hard and soft tissues have been successfully removed by using the Er,Cr:YSGG laser, but there is a controversy about using lasers over conventional tooth surface preparation for bonding aesthetic restoration. Surface roughness and wettability in response to Er,Cr:YSGG laser irradiation are essential properties for restoration longevity. <b>Methods:</b> Fifty-one intact human premolars removed in orthodontic treatment were included in this study and divided into three groups (n=17). The first group (G1) was the control without surface treatment, (G2) was treated with 37% phosphoric acid for 15 seconds, and (G3) was treated with the Er,Cr:YSGG laser using the following parameters: 2 W or 3 W at 20 Hz, 10% air and water ratio using the MZ6 laser tip. The standardization of laser irradiation was accomplished by a computerized numerical control unit. The surface of the samples was evaluated by using a light microscope, profilometer, atomic force microscopy (AFM), SEM, and wettability tests. <b>Results:</b> The SEM examination revealed that the lased enamel surface was clean, irregular, and devoid of a smear layer, while the acid etch surface was relatively smooth and covered with a smear layer. The surface roughness of the lased enamel surface was significantly higher than that of other groups, according to the results of the profilometer as well as the AFM tests. The wettability test showed that the lased enamel surface recorded a significant reduction in the contact angle in comparison to the other groups. <b>Conclusion:</b> It can be concluded that the Er,Cr:YSGG laser can be used as an alternative and safe method to the acid-etching technique for surface treatment.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10843234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune Response in Laser Tattoo Removal: A Systematic Review.","authors":"Arya Tjipta, Hafiz Ramadhan, Rahmi Amelia Lubis","doi":"10.34172/jlms.2023.66","DOIUrl":"10.34172/jlms.2023.66","url":null,"abstract":"<p><p><b>Introduction:</b> The immune response to laser tattoo removal poses a significant challenge in its management, primarily due to its unpredictable nature, which can range from mild hypersensitivity reactions to severe anaphylaxis. Such responses can potentially hinder the effectiveness of laser tattoo removal procedures. Therefore, gaining a comprehensive understanding of the immune response to tattoo removal using laser techniques is of utmost importance to develop more efficient management strategies. This study aims to address this need by analyzing eight carefully selected articles obtained through a thorough literature review. <b>Methods:</b> To explore the immune response associated with laser techniques in tattoo removal, we employed a rigorous research methodology. A thorough literature review was conducted using reputable search engines such as Google Scholar, SagePub, and PubMed to collect relevant articles. Initially, 788 potential articles were identified through this process. Following meticulous scrutiny, only eight articles that met stringent inclusion criteria were selected for our study. This meticulous selection process ensures that the information presented here is derived from high-quality and pertinent research. <b>Results:</b> Based on the analysis of the eight selected articles, our findings illuminate the various immune responses that emerge following tattoo removal using laser techniques. These responses include hypersensitivity reactions, allergic manifestations, and, in certain instances, anaphylaxis. Hypersensitivity reactions typically manifested as erythema, edema, and pruritus, while allergic responses were observed in the form of urticaria. In summary, our study highlights that the immune response to laser tattoo removal primarily elicits hypersensitivity and, in some cases, anaphylaxis reactions. <b>Conclusion:</b> Our study underscores the significance of clinicians being vigilant regarding potential immune responses during laser tattoo removal. It is crucial to closely monitor patients to promptly address any adverse reactions. Further research holds the potential to enhance our understanding, paving the way for improved management strategies that can enhance patient safety and treatment success.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10843227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Intercellular Dynamics: Ultra-Weak Biophoton Emission as a Novel Indicator of Altered Cell Functions and Disease in Oligospermia Mice.","authors":"Arefeh Aryan, Fakhroddin Aghajanpour, Masoomeh Dashtdar, Fatemeh Hejazi, Maryam Salimi, Azar Afshar, Reza Soltani, Ahad Hasan Seyed Hasani, Abbas Aliaghaei, Hojjat-Allah Abbaszadeh, Hasan Mahmoodi, Leila Zahedi, Mohammad-Amin Abdollahifar, Fatemeh Fadaei Fathabadi","doi":"10.34172/jlms.2023.65","DOIUrl":"10.34172/jlms.2023.65","url":null,"abstract":"<p><p><b>Introduction:</b> Biophoton emission, the spontaneous release of photons from living cells, has emerged as an attractive field of research in the study of biological systems. Scientists have recently discovered that changes in biophoton emission could serve as potential indicators of pathological conditions. This intriguing phenomenon suggests that cells might communicate and interact with each other through the exchange of these faint but significant light signals. Therefore, the present study introduces intercellular relationships with biophoton release to detect normal and abnormal cell functions to further achieve cellular interactions by focusing on cell and cell arrangement in disease conditions. <b>Methods:</b> Twenty male mice were assigned to control and busulfan groups. Five weeks after the injection of busulfan, the testis was removed, and then the stereological techniques and TUNEL assay were applied to estimate the histopathology of the testis tissue sections. <b>Results:</b> The findings revealed that the ultra-weak biophoton emission in the control group was significantly lower than in the busulfan group. The oligospermia mice model showed that it significantly changed the spatial arrangement of testicular cells and notably decreased the testis volume, length of seminiferous tubules, and the number of testicular cells. The results of the TUNEL assay showed that the percentage of apoptotic cells significantly increased in the busulfan group. <b>Conclusion:</b> The ultra-weak biophoton emission from testis tissue was reduced in oligospermia mice. As a result, the decline of ultra-weak biophoton can indicate a change in cell arrangement, a decrease in intercellular interaction, and eventually disease.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10843233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Doppler Finding, Cardiovascular Function Assessment, and Fetuses' Survival Following the Fetoscopic Laser in Twin-to-Twin Transfusion Syndrome.","authors":"Tahmineh Ezazi Bojnordi, Laleh Eslamian, Vajiheh Marsoosi, Alireza Golbabaei, Mehrdad Sheikh Vatan, Alireza A Shamshirsaz, Nasim Eshraghi, Marjan Ghaemi","doi":"10.34172/jlms.2023.64","DOIUrl":"10.34172/jlms.2023.64","url":null,"abstract":"<p><p><b>Introduction:</b> This study aimed to evaluate the effectiveness of selective laser photocoagulation of communicating vessels (SLPCV) on cardiac function in twins with twin-to-twin transfusion syndrome (TTTS). <b>Methods:</b> This retrospective cohort study evaluated 178 women with twin pregnancies complicated with TTTS and scheduled for SLPCV between 16 and 26 weeks of gestation. The severity of TTTS was determined by Quintero staging and the severity of cardiovascular disorders by the CHOP (Children's Hospital of Philadelphia) score. Patient survival was evaluated through a one-month-after-birth follow-up of fetuses. <b>Results:</b> The study revealed significant improvements in Doppler indices in both donors and recipients after SLPCV. The CHOP score also significantly decreased after the intervention. One-month-after-birth survival rates were 55.1% in donors and 56.7% in recipients. Some Doppler indexes of fetuses before SLPCV could predict survival until one month after birth. <b>Conclusion:</b> The study suggests that SLPCV can improve cardiac function in fetuses with TTTS and that some Doppler indexes can predict survival outcomes. Additionally, the severity of TTTS can be a powerful indicator of the severity of cardiovascular complications.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10843211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the Diode Laser Wavelengths 445 nm and 810 nm in Gingival Depigmentation – A Clinical Evaluation","authors":"Seyed Masoud Mojahedi Nasab, Matthias Frentzen, Andreas Mayr, Somayeh Rahmani, F. Anbari, J. Meister, Saranaz Azari-Marhabi, Deniz Mojahedi Nasab","doi":"10.34172/jlms.2023.63","DOIUrl":"https://doi.org/10.34172/jlms.2023.63","url":null,"abstract":"Introduction: Nowadays, esthetic appearance plays an important role in the field of dentistry. Discolorations and pigmentations of the gingiva reduce the appearance of a healthy-looking smile. On this occasion, the use of lasers shows a promising approach for a fast and non-aggressive treatment in this field. Different laser wavelengths are being used for gingival depigmentation this clinical study aimed to investigate the effect of the novel laser wavelength (445 nm) in this field and compare it with an 810 nm diode laser. Methods: Two diode laser wavelengths (445 nm and 810 nm) were used for depigmentation. The laser output power chosen was 1 W. An optical fiber with a diameter of 400 µm was used. 21 patients with pigmented gingiva were selected. Depigmentation was carried out in a split-mouth design for a direct comparison of the clinical effect. Outcomes were documented by photograph after one month and six months of follow-up. Results: For each wavelength, 21 volunteers evaluated 21 clinical cases of depigmentation, which means that 441 comparisons were carried out in total regarding the color change from brownish to pinkish. A 100% clarification was achieved for 445 nm. In the 810 nm group, the color change in 44 of 441 cases (10%) could not be clearly identified. No statistically significant difference in pain experience was reported for both laser treatments. Conclusion: The clinical evaluation showed that within the limitations of this study, most of the clinical outcome parameters were highly acceptable by the patients due to mild pain and discomfort for both laser systems.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139171904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Effect of Phosphoric Acid or Er: YAG Laser on the Shear Bond Strength of Orthodontic Brackets to Enamel Surfaces Followed by 980-Laser Assisted Bleaching: An In Vitro Study","authors":"Mohammad Oladzad, N. Chiniforush, R. Bahrami, A. Mirhashemi","doi":"10.34172/jlms.2023.62","DOIUrl":"https://doi.org/10.34172/jlms.2023.62","url":null,"abstract":"Introduction: The residual oxygen remained on tooth surface after bleaching may interfere with adhesion of brackets to enamel surface. This study aimed to evaluate the influence of phosphoric acid and Er: YAG laser as an etching technique on the shear bond strength (SBS) of orthodontic brackets to enamel surfaces after 980-laser-assisted bleaching. Method: A total of 84 human premolars were recruited in the present study. Samples were divided into 6 groups including conventional bleaching with conventional etching, conventional bleaching with Er: YAG laser etching, laser-assisted bleaching with conventional etching, and Laser-assisted bleaching with Er: YAG laser etching, without bleaching with traditional etching, and without bleaching+Er: YAG laser etching. Following thermocycling, the debonding of brackets was conducted using a universal testing machine. Scanning electron microscope (SEM) microscope evaluation and adhesive remnant index (ARI) scores were assessed. The comparison of SBS values between groups was carried out by means of a one-way analysis of variance, followed by post-hoc tests. Results: The non-bleaching with conventional etching group showed the highest SBS mean value (23.45±5.16 MPa), whereas the conventional bleaching with conventional etching group represented the lowest SBS mean value (8.8±3.83 MPa). In all groups, the most common type of failure was classified as either score 1 or score 2. No significant difference was observed in terms of SBS mean between the groups (P=0.165); however, the average SBS of bleached teeth was significantly lower, compared to the non-bleached group (P=0.000). Honeycomb structure and porosity were observed following Er: YAG laser etching on the tooth surface. Conclusion: Increased bond strength of brackets was observed in bleached teeth following Er: YAG laser etching. Therefore, if necessary, bond the brackets on the same day of bleaching will be done, the application of Er:YAG laser as etching technique will can be recommended.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139181253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histologic Evaluation of Effect of Three Wavelengths of Diode Laser on Human Gingival Margins","authors":"Pilar Rocío Blanco Flórez, Helida Helena Avendaño Maz, Josep Arnabat Domínguez, Antonio Jesús España Tost, Jennifer Orozco Páez","doi":"10.34172/jlms.2023.61","DOIUrl":"https://doi.org/10.34172/jlms.2023.61","url":null,"abstract":"Introduction: Available evidence suggests that the response of the intervened tissue is directly linked to the effects generated by the cutting instrument used. To determine the histological findings in gingival tissue margins excised through gingivectomies performed using 450 nm, 940 nm, and 980 nm diode lasers. The present study aimed to determine the histological findings in gingival tissue margins excised through gingivectomies performed using 450 nm, 940 nm, and 980 nm diode lasers. Methods: Gingival tissue samples were collected from 30 patients who had undergone gingivectomy procedures. Each study group comprised 10 patients who willingly provided their samples after providing informed consent. The visualization of histological findings was facilitated through Hematoxylin-Eosin staining. Additionally, variables related to pain and hemostasis were assessed during the intraoperative period. Results: The incision quality was categorized as irregular across all three wavelengths. Histological examination of the epithelial tissue revealed the absence of carbonization and the preservation of cell morphology in over 50% of the resection margin in samples obtained with the 450 nm and 940 nm wavelengths. In the connective tissue, observations included carbonization, collagen coagulation, and basophilia, with the 980 nm wavelength demonstrating the highest percentage of samples displaying collagen coagulation in more than 50% of the resection margin. Conversely, the 450 nm wavelength exhibited the highest degree of preservation of the fibroblast structure. Conclusion: Based on a comprehensive analysis of the study results, it can be inferred that the 450nm and 940nm wavelength lasers tend to produce less thermal damage and better cell preservation when compared to the 980nm wavelength.","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139183708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}