Drug Testing and Analysis最新文献_第4页

Comparison of Microcapillary Blood Sampling Devices for Use in Anti-Doping. 用于反兴奋剂的微毛细管血液采样器的比较。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-10-19 DOI: 10.1002/dta.3818

Laura Lewis, Monika Smith, Kristin Boutard, Matthew Fedoruk, Geoff Miller

{"title":"Comparison of Microcapillary Blood Sampling Devices for Use in Anti-Doping.","authors":"Laura Lewis, Monika Smith, Kristin Boutard, Matthew Fedoruk, Geoff Miller","doi":"10.1002/dta.3818","DOIUrl":"https://doi.org/10.1002/dta.3818","url":null,"abstract":"Blood collection is an important facet of anti-doping testing, forming the basis of the hematological module of the athlete biological passport (ABP). Presently, whole blood is collected via venepucture under standardized conditions, in accordance with the World Anti-doping Agency's International Standard for Testing and Investigations (ISTI). Advances in capillary whole blood collection technology now afford the ability to collect microvolumetric capillary whole blood from the upper arm (or other suitable vascular location such as the abdomen) that is \"needle-free\" and virtually painless. Previous work has demonstrated the suitability and feasibility of capillary whole blood compared with venous using the Tasso+ device. Recently, other microcapillary devices have received FDA approval increasing the options available to the anti-doping community. The present study extends previous work, by collecting microliter capillary whole blood samples via two different FDA approved devices (RedDrop and the Tasso+ device) to compare with venous blood collected simultaneously. Ten participants provided three matched blood samples (2× capillary and 1× venous) in accordance with WADA ABP guidelines for blood sample collection, for a total of 30 samples. Capillary samples from both devices showed excellent laboratory agreement with venous blood for all CBC parameters, with the exception of platelets. Excellent laboratory agreement was also observed between the two microcapillary collection devices. Irrespective of the device, microcapillary blood collection provides a valid alternative to venous blood collection for ABP purposes.","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid and Corticosteroid Biomarkers Under the Influence of Bisphosphonates. 双膦酸盐影响下的血脂和皮质类固醇生物标志物

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-10-15 DOI: 10.1002/dta.3811

Kathy Tou, Adam Cawley, Glenys Noble, Jaymie Loy, David Bishop, John Keledjian, Kireesan Sornalingam, Stacey Richards, Shanlin Fu

{"title":"Lipid and Corticosteroid Biomarkers Under the Influence of Bisphosphonates.","authors":"Kathy Tou, Adam Cawley, Glenys Noble, Jaymie Loy, David Bishop, John Keledjian, Kireesan Sornalingam, Stacey Richards, Shanlin Fu","doi":"10.1002/dta.3811","DOIUrl":"https://doi.org/10.1002/dta.3811","url":null,"abstract":"Detecting the use of bisphosphonates (BPs) in equine athletes is of interest to regulators and laboratories due to the threat to welfare issues for the potential to provide analgesic effects and manipulating bone structure. The detection of BPs in biological matrices is challenging due to erratic biological elimination and inconsistent analytical recoveries. Therefore, complementary approaches are needed to provide evidence of their misuse in racehorses. BPs have two sub-classes: nitrogenous and non-nitrogenous. This study investigated plasma elimination following administration of one example from each sub-class, together with changes in endogenous eicosanoid and corticosteroids. Zoledronic acid (ZA) and tiludronic acid (TA) were administered by IV infusion to 8 thoroughbred horses with an 11-month washout period between each administration. Sample preparation for quantification of BPs by liquid chromatography-tandem mass spectrometry (LC-MS/MS) utilised a two-step solid phase extraction (SPE) consisting of polymeric reversed-phase followed by weak anion exchange prior to derivatisation using trimethyl orthoacetate. Endogenous biomarkers were analysed after protein precipitation and SPE with polymeric reversed-phase prior to liquid chromatography-high resolution mass spectrometry (LC-HRMS) using data independent acquisition. The LC-MS/MS analysis showed ZA was undetectable after 8 h post-administration while TA was detected up to the final collection point of 28 days post-administration. The LC-HRMS analysis utilised targeted (i.e., prior inclusion list of compounds) approaches to monitor level changes of eicosanoid and corticosteroid biomarkers. Putative biomarkers were identified and now subject to validation for translation into routine sample analysis for improved retrospectivity to detecting BP misuse in equine plasma.","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Waxy- or Putty-Like Materials as a Novel Drug Preparation for Synthetic Cannabinoid Receptor Agonists: Detection in Prisons and In Vitro Cannabinoid Receptor Activity. 蜡状或腻子状材料作为合成大麻素受体激动剂的新型药物制剂：在监狱中的检测和体外大麻素受体活性。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-10-15 DOI: 10.1002/dta.3817

Axelle Timmerman, Marie H Deventer, Rachael Andrews, Robert Reid, Victoria Marland, Darren Edwards, Christopher R Pudney, Niamh Nic Daéid, Christophe P Stove, Caitlyn Norman

{"title":"Waxy- or Putty-Like Materials as a Novel Drug Preparation for Synthetic Cannabinoid Receptor Agonists: Detection in Prisons and In Vitro Cannabinoid Receptor Activity.","authors":"Axelle Timmerman, Marie H Deventer, Rachael Andrews, Robert Reid, Victoria Marland, Darren Edwards, Christopher R Pudney, Niamh Nic Daéid, Christophe P Stove, Caitlyn Norman","doi":"10.1002/dta.3817","DOIUrl":"https://doi.org/10.1002/dta.3817","url":null,"abstract":"After the Scottish Prison Service (SPS) introduced mail photocopying procedures in December 2021, a shift in smuggling methods was observed for synthetic cannabinoid receptor agonists (SCRAs) and other new psychoactive substances (NPS) from drug-infused papers back to traditional sample matrices (e.g., tablets and powders), although new matrices also emerged. This study reports on waxy- or putty-like materials as a novel drug preparation for SCRAs and other drugs seized from UK prisons. In 2023, 22 of these new preparations were seized from Scottish prisons, with eight found in sealed vape pods. The materials were positive for SCRAs, phytocannabinoids, novel benzodiazepines, and/or gabapentinoids. Additionally, 11 preparations were seized from an English prison, all containing the SCRAs MDMB-4en-PINACA and MDMB-INACA. MDMB-INACA was pharmacologically characterized using in vitro CB1 and CB2 bioassays, revealing moderate efficacy but low potency at CB1. Furthermore, the in vitro CB1 bioassay was also used to evaluate the CB1 activating potential of extracts from eight seized samples. Six of these showed high CB1 activity, whereas the samples lacking SCRAs or containing only MDMB-INACA showed no or only weak CB1 activity, respectively. Lastly, applying the bioassay as an activity-based \"untargeted\" screening method effectively identified the presence of SCRAs in one waxy preparation, which was initially not detected by gas chromatography-mass spectrometry (GC-MS). This underscores the effectiveness of the bioassay for evaluating these new waxy- or putty-like materials for the presence of SCRAs.","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Demonstration of an End-To-End Workflow Using Atmospheric Solids Analysis Probe-Mass Spectrometry (ASAP-MS) With Real-Time Sample Recognition Software for the Identification of Falsified and Substandard Pharmaceutical Tablets. 利用大气固体分析探针质谱法 (ASAP-MS) 和实时样品识别软件识别假冒伪劣药片的端到端工作流程演示。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-10-12 DOI: 10.1002/dta.3816

Fiona Smillie, Weronika Glinka, Christopher Henry, Adam McCudden, Jennifer Thorpe, Stephen W Holman

{"title":"Demonstration of an End-To-End Workflow Using Atmospheric Solids Analysis Probe-Mass Spectrometry (ASAP-MS) With Real-Time Sample Recognition Software for the Identification of Falsified and Substandard Pharmaceutical Tablets.","authors":"Fiona Smillie, Weronika Glinka, Christopher Henry, Adam McCudden, Jennifer Thorpe, Stephen W Holman","doi":"10.1002/dta.3816","DOIUrl":"https://doi.org/10.1002/dta.3816","url":null,"abstract":"Counterfeit pharmaceuticals are a subclass of falsified and substandard medicines. They are illicit products, purporting to be genuine medicines, that are made and sold by criminal organisations. They represent a significant risk to patient safety, as well as a financial and reputational threat to the companies who make the genuine medicines. It is essential to have analytical methods to determine if suspect samples seized by law enforcement agencies are counterfeit, with mass spectrometry (MS) being a commonly used technique in forensic cases. Speed-to-answer is vital to enable law enforcement agencies to progress investigations, as well as for pharmaceutical companies so that they can notify health authorities of the circulation of counterfeit medicines. In this work, an atmospheric solids analysis probe (ASAP)-MS was assessed as a fast and simple-to-use approach to analyse tablets on a commercially available instrument. Complementing the analytics with real-time sample recognition software demonstrated that the classification of tablets as authentic or counterfeit could be achieved quickly (< 2 min) and without the need for MS interpretation skills. Authentication of five tablets (two authentic pharmaceuticals, one placebo and two counterfeits containing the correct active pharmaceutical ingredient [API] but at lower quantities than in the genuine medicine and with different excipient contents) of unknown origin was achieved with 100% success. This creates the opportunity to deploy the end-to-end workflow as a tool for non-scientists, such as law enforcement officers and border control staff, for use in-territory to obtain fast answers and make data-led decisions to control the illegal trading of medicines.","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The 42nd Manfred Donike Workshop on Doping Analysis. 第 42 届 Manfred Donike 兴奋剂分析研讨会。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-10-03 DOI: 10.1002/dta.3815

Mario Thevis

引用次数: 0

Long-Term Excretion of Roxadustat in Urine. 尿液中罗沙司他（Roxadustat）的长期排泄。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-10-01 DOI: 10.1002/dta.3813

Tim Sobolevsky, Matthew Fedoruk, Frank Dellanna, Hans Geyer, Brian Ahrens, Mario Thevis

{"title":"Long-Term Excretion of Roxadustat in Urine.","authors":"Tim Sobolevsky, Matthew Fedoruk, Frank Dellanna, Hans Geyer, Brian Ahrens, Mario Thevis","doi":"10.1002/dta.3813","DOIUrl":"https://doi.org/10.1002/dta.3813","url":null,"abstract":"Roxadustat (FG-4592), an orally active hypoxia-inducible factor prolyl hydroxylase stabilizer, has been shown to enhance erythropoiesis by increasing endogenous erythropoietin. It is indicated for the treatment of anemia and chronic kidney disease and is approved for clinical use in several countries, including the European Union, Japan and others. Due to its reasonably anticipated performance-enhancing effect in athletes, roxadustat is prohibited for use in sports at all times. A few cases of adverse analytical findings in routine doping controls have been reported worldwide, some of which were claimed to be the result of contaminated dietary supplements. The present study offers new data demonstrating the long-term excretion pattern of roxadustat. Even after a single-dose administration, roxadustat can remain detectable in urine for 8 months, albeit at very low concentrations (<10 pg/mL). Following three times a week treatment with 70 to 100 mg of roxadustat, the drug was still detectable in the urine of anemic patients for between 9 and 18 months after treatment was discontinued. Lastly, an athlete who admitted use of roxadustat for almost a year (50 mg 3 to 5 times a week) has now tested positive multiple times over the course of 15 months (the first test being 12 months after the drug was discontinued), with an estimated concentration of roxadustat between 3 and 8 pg/mL. Altogether, these findings indicate the unusually prolonged terminal excretion kinetics of roxadustat, a property that testing authorities should consider in their results management process.","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Transcriptomic Biomarkers for Detection of the Recombinant Human Erythropoietin (rHuEPO) MirCERA in Horses. 用于检测马体内重组人促红细胞生成素 (rHuEPO) MirCERA 的新转录组生物标记物。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-09-25 DOI: 10.1002/dta.3812

Benoit Loup, François André, Nicolas Leuenberger, Alexandre Marchand, Agnès Barnabé, Vivian Delcourt, Patrice Garcia, Marie-Agnès Popot, Ludovic Bailly-Chouriberry

{"title":"New Transcriptomic Biomarkers for Detection of the Recombinant Human Erythropoietin (rHuEPO) MirCERA in Horses.","authors":"Benoit Loup, François André, Nicolas Leuenberger, Alexandre Marchand, Agnès Barnabé, Vivian Delcourt, Patrice Garcia, Marie-Agnès Popot, Ludovic Bailly-Chouriberry","doi":"10.1002/dta.3812","DOIUrl":"https://doi.org/10.1002/dta.3812","url":null,"abstract":"Detection and monitoring of biomarkers related to doping is particularly suitable for the development of analytical strategies dedicated to indirect detection of banned substances. Previous studies in horses have already allowed the investigation of transcriptomic biomarkers in equine blood associated with reGH and rHuEPO administrations. Our most recent developments continue to focus on the discovery and monitoring of transcriptomic biomarkers for the control of ESAs, and a collaborative study with WADA-accredited doping control laboratories has recently been initiated to conduct a pilot study. In humans, three mRNAs (ALAS2, CA1, and SLC4A1) were previously observed to be differentially expressed after blood doping and were associated with immature red blood cells, the so-called circulating reticulocytes. In horses, circulating reticulocytes are rarely observed even after rHuEPO administration. With the improved primers that detect the equine orthologues of the human mRNAs from the ALAS2, CA1, and SLC4A1 genes, we can now report the first evidence of the detection of the three biomarkers in equine blood. In addition, an upregulation of the mRNA levels of the three genes was observed after analysis of blood samples collected from MirCERA-treated animals, with kinetics similar to those previously documented in humans. Our data suggest that ALAS2 and CA1 are promising indirect biomarkers for the detection of recombinant EPO abuse in horses, as observed in humans.","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Equine Doping Controls of Thymosin β $$ beta $$ 4: A Population Study and Strategy for Misuse Detection. 胸腺肽 β$ beta$ 4 的马匹兴奋剂控制：群体研究与滥用检测策略。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-09-24 DOI: 10.1002/dta.3806

Vivian Delcourt, Patrice Garcia, Benjamin Chabot, Nina Aber, Mylène Pescher, Marie Cacault, Priscilla Scholtes, Benoit Loup, Agnès Barnabé, Marie-Agnès Popot, Ludovic Bailly-Chouriberry

{"title":"<ArticleTitle xmlns:ns0=\"http://www.w3.org/1998/Math/MathML\">Equine Doping Controls of Thymosin <ns0:math> <ns0:semantics><ns0:mrow><ns0:mi>β</ns0:mi></ns0:mrow> <ns0:annotation>$$ beta $$</ns0:annotation></ns0:semantics> </ns0:math> 4: A Population Study and Strategy for Misuse Detection.","authors":"Vivian Delcourt, Patrice Garcia, Benjamin Chabot, Nina Aber, Mylène Pescher, Marie Cacault, Priscilla Scholtes, Benoit Loup, Agnès Barnabé, Marie-Agnès Popot, Ludovic Bailly-Chouriberry","doi":"10.1002/dta.3806","DOIUrl":"https://doi.org/10.1002/dta.3806","url":null,"abstract":"Thymosin <math> <semantics><mrow><mi>β</mi></mrow> <annotation>$$ beta $$</annotation></semantics> </math> 4 (TB4) is a ubiquitous, highly conserved and abundant peptide among mammals with a critical role in cytoskeleton organization. In spite of its yet non-authorized use as a medicine and being forbidden by the IFHA, the FEI, and the WADA, intelligence and doping control laboratories reported numerous products available online claiming to contain a synthetic acetylated fragment of TB4 or TB4 itself, promoted as a growth factor with regenerative properties. In this article, the first estimation of the endogenous TB4 concentration in racing horses' blood samples was performed through a population study. We reveal that this concentration does not significantly depend on gender, age, nor horse breed. We highlight that the TB4 concentration increases significantly and rapidly in plasma stored at 4°C when not separated from blood cells due to cell lysis. Finally, we also demonstrate that the detection of a non-natural synthesis impurity is possible in equine plasma after a single dose administration of a TB4 containing product to a horse.","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142306786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skeletal Muscle Memory: An Update From the Antidoping Perspective. 骨骼肌记忆：从反兴奋剂角度看最新进展。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-09-24 DOI: 10.1002/dta.3804

Claire Traversa

{"title":"Skeletal Muscle Memory: An Update From the Antidoping Perspective.","authors":"Claire Traversa","doi":"10.1002/dta.3804","DOIUrl":"https://doi.org/10.1002/dta.3804","url":null,"abstract":"This narrative review explores the concept of muscle memory, focusing on the physiological and biochemical mechanisms underlying information retention in skeletal muscle tissue as it relates to antidoping. The discussion encompasses the role of satellite cells (SCs) in myonuclei recruitment, resulting in increased myonuclear density and heightened muscle protein turnover. The myonuclear domain theory suggests that myonuclei acquired during hypertrophy may persist, contributing to enhanced muscle protein synthesis (MPS) and potential benefits of muscle memory. The impact of sustained training, protein intake, and resistance exercise on muscle memory, especially in elite athletes, is considered. The review also delves into the influence of anabolic androgenic steroids (AAS) on muscle tissue, highlighting their role in elevating the performance threshold and supporting recovery during intense training through increased muscle protein turnover rates. Additionally, genetic and epigenetic modifications, such as DNA methylation, are explored as potential contributors to muscle memory. The complex interplay of continuous training, AAS use, and genetic factors offers avenues for further research, especially in the context of antidoping efforts. The understanding of muscle memory has implications for maintaining performance gains and addressing ethical challenges in sports.","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suitability of Hair Testing in Medical Dispensing Errors: A Fatal Case of Gliclazide Poisoning. 毛发检测在医疗配药错误中的适用性：一例格列齐特中毒死亡病例。

IF 2.6 3区医学

Drug Testing and Analysis Pub Date : 2024-09-22 DOI: 10.1002/dta.3809

S Riess, N Klinger, O Roussel, M Cheze, R Gonçalves, V Cirimele

{"title":"Suitability of Hair Testing in Medical Dispensing Errors: A Fatal Case of Gliclazide Poisoning.","authors":"S Riess, N Klinger, O Roussel, M Cheze, R Gonçalves, V Cirimele","doi":"10.1002/dta.3809","DOIUrl":"https://doi.org/10.1002/dta.3809","url":null,"abstract":"A patient was prescribed a new treatment, 40 mg furosemide. Her pharmacist mistakenly dispensed 40 mg gliclazide instead. After 3 weeks of treatment, the patient was found in a coma, was hospitalised and died after a week. Hair sample was collected during post-mortem examination and tested for gliclazide on three 2 cm sections, starting at the root end. Hair strands were decontaminated, segmented and incubated in the presence of diazepam-d5, and a solid-liquid extraction has been performed. Finally, toxicological analyses were performed by UFLC Shimadzu Prominence - MS/MS Sciex 6500 QTRAP. Gliclazide was found on the proximal (238 pg/mg), median (77 pg/mg) and distal (69 pg/mg) segments. The concentration in the proximal segment was largely higher than in the other two, which demonstrates repeated intake of gliclazide by the patient during at least the last 2 months before death. The lower concentrations in the medial and distal segments, as well as a part of the concentration in the proximal segment, may be linked to external hair contamination. These could be due to either radial sweat diffusion, possibly occurring in the last week hospitalisation of the patient, or to biological fluids contamination during post-mortem examination. This case illustrates the opportunity to confirm slow and fatal chronic poisoning by gliclazide using hair analysis and shows the benefit of hair testing for the investigation of medical or dispensing errors.","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142277633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0