Effects of the Dietary Supplement 5α-Hydroxy-Laxogenin in the Orchiectomized Rat Model.

IF 2.6 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Drug Testing and Analysis Pub Date : 2025-03-04 DOI:10.1002/dta.3881

Daniel Derwand, Oliver Zierau, Clemens Alexander Wolf, Gerhard Wolber, Annekathrin Martina Keiler

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引用次数: 0

Abstract

Dietary supplements used by recreational and elite athletes for performance enhancement might contain undeclared, unlawfully added ingredients. One of those ingredients is 5α-hydroxy-laxogenin, which is sold in dietary supplements marketed as a natural compound with anabolic effects. It has been shown that 5α-hydroxy-laxogenin is not naturally occurring, but rather of synthetic origin. Previously, we observed that 5α-hydroxy-laxogenin can bind to and activate the androgen receptor in a cell-based bioassay. To investigate its androgenic potential in vivo, we treated orchiectomized rats with three different dosages of 5α-hydroxy-laxogenin for 2 weeks. Effects were neither observed on the wet weights of the androgen target tissues prostate, seminal vesicle or penis nor on the wet weights of the anabolic target tissue musculus levator ani or on skeletal hindlimb muscles. Au contraire, significantly higher atrophy was seen for some of the target tissues in the animals treated with the highest 5α-hydroxy-laxogenin dosage (36 mg/kg bw). While in silico docking supports the androgen receptor binding previously observed in vitro, we observed neither androgenic nor anabolic effects of 5α-hydroxy-laxogenin in vivo in castrated male rats.

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膳食补充剂5α-羟基-松弛素对去睾丸大鼠模型的影响。

休闲和精英运动员为提高成绩而使用的膳食补充剂可能含有未申报的、非法添加的成分。其中一种成分是5α-羟基松弛素，它作为一种具有合成代谢作用的天然化合物在膳食补充剂中销售。研究表明，5α-羟基松弛素不是自然产生的，而是人工合成的。在此之前，我们观察到5α-羟基laxogenin可以结合并激活雄激素受体。为了研究其在体内的雄激素作用，我们给去睾丸的大鼠注射了三种不同剂量的5α-羟基松弛素2周。对雄激素靶组织前列腺、精囊或阴茎的湿重没有影响，对合成代谢靶组织提肛肌或后肢骨骼肌的湿重也没有影响。相反，在5α-羟基-松弛素最高剂量（36 mg/kg bw）处理的动物中，一些靶组织出现了明显更高的萎缩。虽然硅对接支持先前在体外观察到的雄激素受体结合，但我们在阉割的雄性大鼠体内没有观察到5α-羟基laxogenin的雄激素作用和合成代谢作用。

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来源期刊

Drug Testing and Analysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

5.90

自引率

24.10%

发文量

191

审稿时长

2.3 months

期刊介绍： As the incidence of drugs escalates in 21st century living, their detection and analysis have become increasingly important. Sport, the workplace, crime investigation, homeland security, the pharmaceutical industry and the environment are just some of the high profile arenas in which analytical testing has provided an important investigative tool for uncovering the presence of extraneous substances. In addition to the usual publishing fare of primary research articles, case reports and letters, Drug Testing and Analysis offers a unique combination of; ‘How to’ material such as ‘Tutorials’ and ‘Reviews’, Speculative pieces (‘Commentaries’ and ‘Perspectives'', providing a broader scientific and social context to the aspects of analytical testing), ‘Annual banned substance reviews’ (delivering a critical evaluation of the methods used in the characterization of established and newly outlawed compounds). Rather than focus on the application of a single technique, Drug Testing and Analysis employs a unique multidisciplinary approach to the field of controversial compound determination. Papers discussing chromatography, mass spectrometry, immunological approaches, 1D/2D gel electrophoresis, to name just a few select methods, are welcomed where their application is related to any of the six key topics listed below.