Journal of immunoassay最新文献_第3页

Detection of interleukin-12 (IL-12)-secreting cells in normal mice with enzyme-linked immunospot (ELISPOT) assay. 酶联免疫斑点法(ELISPOT)检测正常小鼠白细胞介素12 (IL-12)分泌细胞。

Journal of immunoassay Pub Date : 2000-02-01 DOI: 10.1080/01971520009349497

Y Okamoto, Y Gotoh, Y Kobayashi, A Mizuno, H Tokui, M Nishida

引用次数: 1

Investigation of electroplated conducting polymers as antibody receptors in immunosensors. 电镀导电聚合物作为免疫传感器抗体受体的研究。

Journal of immunoassay Pub Date : 2000-02-01 DOI: 10.1080/01971520009349499

R A Porter

引用次数: 9

Serum thyroxine binding capacity-dependent bias in an automated free thyroxine assay. 自动游离甲状腺素测定中血清甲状腺素结合能力依赖性偏倚。

Journal of immunoassay Pub Date : 1999-11-01 DOI: 10.1080/01971529909349351

N D Christofides, E Wilkinson, M Stoddart, D C Ray, G J Beckett

引用次数: 24

Evaluation of a new troponin I method on the Bayer Immuno 1 immunoassay analyser. 一种新的肌钙蛋白I方法在Bayer Immuno 1免疫分析分析仪上的评价。

Journal of immunoassay Pub Date : 1999-11-01 DOI: 10.1080/01971529909349354

G H Clark, S R Kennon, C P Price

{"title":"Evaluation of a new troponin I method on the Bayer Immuno 1 immunoassay analyser.","authors":"G H Clark, S R Kennon, C P Price","doi":"10.1080/01971529909349354","DOIUrl":"https://doi.org/10.1080/01971529909349354","url":null,"abstract":"We have evaluated the analytical and clinical performance of an automated immunoassay for serum cardiac troponin I (Bayer Immuno 1TM, Bayer Diagnostics, Tarrytown, NY). The between batch imprecision was found to be between 1.2 and 3.2% over the concentration range 2.5 - 34.0 microg/L. The analytical range obtained from duplicate analysis of patient samples and defined as a coefficient of variation of 10% or less was 0.3 - 200 microg/L. The detection limit was found to be less than 0.1 microg/L. A method comparison with the Dade Stratus method (Dade Behring, Wilmington, DE) yielded regression statistics with a slope of 0.705 and an intercept of -0.260. An analysis of samples from 40 patients with renal failure demonstrated six with detectable levels of troponin I (0.2 - 1.9 microg/L). Samples from patients with paraproteinaemia did not demonstrate detectable troponin I (from n = 30); however, two patients with elevated rheumatoid factor titers (from n = 20) demonstrated a detectable amount of troponin I (0.1 and 0.2 microg/L). In a study of 100 patients admitted with acute chest pain and a diagnosis of unstable angina, 6 were subsequently diagnosed as having suffered a myocardial infarction. On admission the sensitivity and specificity of the troponin I results were 26.7% and 94.7%, respectively, moving to 100% and 83% 12 hours after admission.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"20 4","pages":"253-73"},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529909349354","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21452535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Validation of an ELISA for the quantitation of lanoteplase, a novel plasminogen activator. 一种新型纤溶酶原激活剂lanoteplase的ELISA定量验证。

Journal of immunoassay Pub Date : 1999-11-01 DOI: 10.1080/01971529909349353

B Stouffer, S Habte, N Vachharajani, L Tay

{"title":"Validation of an ELISA for the quantitation of lanoteplase, a novel plasminogen activator.","authors":"B Stouffer, S Habte, N Vachharajani, L Tay","doi":"10.1080/01971529909349353","DOIUrl":"https://doi.org/10.1080/01971529909349353","url":null,"abstract":"An ELISA was developed and validated for the quantitation of lanoteplase in human citrated plasma. The ELISA employed a monoclonal anti-lanoteplase antibody absorbed onto 96-well microtiter plates to capture lanoteplase in citrated human plasma samples containing PPACK, a protease inhibitor. The captured lanoteplase was detected using a biotinylated rabbit anti-lanoteplase polyclonal antibody. The standard curve range in human plasma for the ELISA was 7-100 ng/ml. Assessment of individual standard curve variability indicated reproducible responses with r2 values of > or = 0.985. The accuracy (% DEV) and precision (%RSD) estimates for the ELISA based on the predicted values from quality control (QC) samples were within 7.3% and 11%, respectively. Cross-reactivity with t-PA was determined to be less than 11% by ELISA. The stability of lanoteplase was established in human citrated PPACK plasma for 24 hours at 4 degrees C, for 2 months at -20 degrees C, for 22 months at -70 degrees C, three weeks at room temperature, and through four freeze/thaw cycles. To quantify lanoteplase plasminogen activator (PA) activity, a commercially available chromogenic activity assay was also validated. This method and its application is described briefly here. The lanoteplase ELISA as well as the commercial activity method were successfully employed to evaluate the pharmacokinetic parameters of lanoteplase in support of clinical Phase II/III studies.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"20 4","pages":"237-52"},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529909349353","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21452534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Monoclonal antibody-based sensitive enzyme-linked immunosorbent assay for murine serum amyloid A. 基于单克隆抗体的小鼠血清淀粉样蛋白A敏感酶联免疫吸附测定。

Journal of immunoassay Pub Date : 1999-11-01 DOI: 10.1080/01971529909349352

T Yamada, T Fukuda, A Wada, Y Itoh

{"title":"Monoclonal antibody-based sensitive enzyme-linked immunosorbent assay for murine serum amyloid A.","authors":"T Yamada, T Fukuda, A Wada, Y Itoh","doi":"10.1080/01971529909349352","DOIUrl":"https://doi.org/10.1080/01971529909349352","url":null,"abstract":"Enzyme-linked immunosorbent assay (ELISA) methods for measuring murine serum amyloid A (SAA), a representative acute phase reactant, were developed utilizing a newly produced monoclonal antibody. Two site-ELISA, in which the monoclonal antibody was used as the captured antibody, was sensitive enough to determine the SAA concentration in mice at the steady state. Direct binding ELISA, in which the sample SAA bound to the plastic wells was detected by the antibody, was simple and suitable for measuring the elevated SAA, but could not analyze the resting level of SAA because of the need for high dilution in plasma samples. Plasma SAA concentrations were measured in ten ICR mice on the day of purchase and at the end of seven days of ordinary rearing. The SAA concentration of one animal decreased from 1.6 to 0.5 mg/l during a week, while the others had no obvious changes. The plasma SAA of the ten animals after one week of rearing ranged from 0.3 to 0.8 mg/l with a mean of 0.47. These mice, two days after 10 microg lipopolysaccharide were given, had increased SAA values up to a mean of 300 mg/l, though with variations between animals.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"20 4","pages":"223-35"},"PeriodicalIF":0.0,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529909349352","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21452532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Laser light scattering immunoassay for malaria. 疟疾激光散射免疫分析。

Journal of immunoassay Pub Date : 1999-08-01 DOI: 10.1080/01971529909349346

P Bhakat, A Roy, K B Roy, A Saxena, H B Bohidar

引用次数: 7

A comparison of performances of four enzymes used in ELISA with special reference to beta-lactamase. 酶联免疫吸附试验中四种酶的性能比较，以β -内酰胺酶为例。

Journal of immunoassay Pub Date : 1999-08-01 DOI: 10.1080/01971529909349349

M I Khatkhatay, M Desai

引用次数: 18

Fluorescence polarization assay: application to the diagnosis of bovine brucellosis in Argentina. 荧光偏振法在阿根廷牛布鲁氏菌病诊断中的应用。

Journal of immunoassay Pub Date : 1999-08-01 DOI: 10.1080/01971529909349347

L Samartino, R Gregoret, D Gall, K Nielsen

{"title":"Fluorescence polarization assay: application to the diagnosis of bovine brucellosis in Argentina.","authors":"L Samartino, R Gregoret, D Gall, K Nielsen","doi":"10.1080/01971529909349347","DOIUrl":"https://doi.org/10.1080/01971529909349347","url":null,"abstract":"A homogeneous fluorescence polarization assay (FPIA) for detection of bovine antibody to Brucella abortus was validated in Argentina Sera were defined based on their reactivity in the buffered antigen plate agglutination test (BPAT) and the competitive enzyme immunoassay (CELISA). Sera negative in these tests were collected from farms without evidence of brucellosis (n=733). Sera positive in the two tests were collected from cattle on farms from which B. abortus was isolated from at least one animal (n=1039). Sera from cattle vaccinated 26, 89, 240 and 272 days previously with B. abortus strain 19 were collected and tested. A cut-off value of 87 mP was determined for the FPIA, resulting in relative sensitivity and specificity values of 98.1 and 99.6%. The specificity for B. abortus strain 19 vaccinated cattle was 64.9% (26 days post vaccination, DPV), 92.1% (89 DPV), 98.6% (242 DPV) and 97.1% (272 DPV). These values were compared to those obtained with the BPAT, the CELISA, the indirect ELISA, the complement fixation test and the 2-mercaptoethanol agglutination test. Sera from 18 cattle which were vaccinated and revaccinated with B. abortus strain 19 were also tested by the same assays and the FPIA was found to be 100% specific. The use of the FPIA as a diagnostic test for brucellosis is discussed.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"20 3","pages":"115-26"},"PeriodicalIF":0.0,"publicationDate":"1999-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529909349347","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21323166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

A method for correcting for the variability of inhibitory effects of soluble human interleukin 1 receptor II measured by different ELISAS. 一种校正不同elisa测定的可溶性人白细胞介素1受体II抑制效应变异性的方法。

Journal of immunoassay Pub Date : 1999-08-01 DOI: 10.1080/01971529909349350

T Krakauer, H Krakauer

引用次数: 3