Journal of immunoassay最新文献_第4页

Detection of zooplankton prey in squid paralarvae with immunoassay. 用免疫分析法检测乌贼幼虫的浮游动物猎物。

Journal of immunoassay Pub Date : 1999-08-01 DOI: 10.1080/01971529909349348

J D Venter, S van Wyngaardt, J A Verschoor, M R Lipiński, H M Verheye

{"title":"Detection of zooplankton prey in squid paralarvae with immunoassay.","authors":"J D Venter, S van Wyngaardt, J A Verschoor, M R Lipiński, H M Verheye","doi":"10.1080/01971529909349348","DOIUrl":"https://doi.org/10.1080/01971529909349348","url":null,"abstract":"Sustainable management of economically important squid requires monitoring of changes in their abundance, which are related inter alia, to their success in the food chain. The highest mortality is expected in the paralarval stages, which are prone to starvation. Causes of starvation may be linked to the lack of suitable prey. A multiple detection system was developed for the simultaneous identification of five putative zooplankton prey in the guts of paralarval Chokka squid, Loligo vulgaris reynaudii, by employing polyclonal rabbit antisera in conjunction with solid phase immunoassays. Specificities of antisera were validated by ELISA screening against different zooplankton taxa. Cross-reactions observed with ELISA were minimized through manipulation of antibody and antigen concentrations resulting in more specific detection of target prey antigens when used in an immunodot assay. Application of this optimised immunoassay detected multiple predation in paralarval squid samples collected from diverse areas in the Agulhas Bank ecosystem on the south coast of South Africa.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"20 3","pages":"127-49"},"PeriodicalIF":0.0,"publicationDate":"1999-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529909349348","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21323167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Blocking of non-specific sorption in ELISA on formylated polystyrene beads. 甲酰化聚苯乙烯珠在ELISA中非特异性吸附的阻断。

Journal of immunoassay Pub Date : 1999-02-01 DOI: 10.1080/01971529909349311

V V Shmanai

引用次数: 8

The detection of pyrogens in sera from patients with symptoms of sepsis using an ex vivo whole blood culture assay. 用体外全血培养法检测脓毒症患者血清中的热原。

Journal of immunoassay Pub Date : 1999-02-01 DOI: 10.1080/01971529909349310

E J Pool, P Bouic

{"title":"The detection of pyrogens in sera from patients with symptoms of sepsis using an ex vivo whole blood culture assay.","authors":"E J Pool, P Bouic","doi":"10.1080/01971529909349310","DOIUrl":"https://doi.org/10.1080/01971529909349310","url":null,"abstract":"The aim of this study was to investigate whether the ex vivo whole blood culture (WBC) assay system can be used to detect pyrogens in blood from patients with symptoms of sepsis. Blood samples from 35 patients with symptoms of sepsis were assayed for bacterial contamination using the radiometric blood culture assay. Serum from the same patients were screened for IL-6, C-reactive protein (CRP) and pyrogens using the whole blood culture assay. Serum samples from 26 patients tested positive for pyrogens. Of the 26 patients with pyrogenic serum, 15 had elevated serum IL-6 levels and 19 had elevated CRP levels. Only two of the samples had positive blood cultures as detected by the routine radiometric assay. Both of these patients had high serum CRP and pyrogen levels, while only one of them had an elevated serum IL-6 level. These results show that the WBC is very sensitive in detecting pyrogens in serum of patients. This technique can be a useful tool to quantitate pyrogens in sera from patients with symptoms of sepsis and to determine whether their clinical symptoms are caused by pyretic substances in their circulatory system.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"20 1-2","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"1999-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529909349310","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21095924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Production and characterization of monoclonal antibodies specific to atrazine group compounds: effects of coating ligand structure on the variation of sensitivity and specificity. 针对阿特拉津族化合物的单克隆抗体的制备与鉴定:包衣配体结构对敏感性和特异性变化的影响。

Journal of immunoassay Pub Date : 1999-02-01 DOI: 10.1080/01971529909349314

M J Choi, Y Jo, J Choi, C Y Kang, C T Han

引用次数: 13

Anti-idiotypic sera against monoclonal anti-porcine growth hormone antibodies: production in rabbits and characterization of specificity. 兔抗猪生长激素单克隆抗体抗独特型血清的制备及特异性表征。

Journal of immunoassay Pub Date : 1999-02-01 DOI: 10.1080/01971529909349313

C M Cahill, J T Rogers

引用次数: 0

A capture enzyme-linked immunosorbent assay for species-specific detection of Bothrops venoms. 一种捕获酶联免疫吸附法用于种特异性检测Bothrops毒液。

Journal of immunoassay Pub Date : 1999-02-01 DOI: 10.1080/01971529909349316

L G Heneine, M R Araújo dos Santos, A Dutra de Carvalho, S da Silva Gontijo

引用次数: 4

The optimisation of a murine TNF-alpha ELISA and the application of the method to other murine cytokines. 小鼠tnf - α酶联免疫吸附试验的优化及其在其他小鼠细胞因子检测中的应用。

Journal of immunoassay Pub Date : 1999-02-01 DOI: 10.1080/01971529909349312

A M Yates, S J Elvin, D E Williamson

{"title":"The optimisation of a murine TNF-alpha ELISA and the application of the method to other murine cytokines.","authors":"A M Yates, S J Elvin, D E Williamson","doi":"10.1080/01971529909349312","DOIUrl":"https://doi.org/10.1080/01971529909349312","url":null,"abstract":"Cytokines occur in biological systems at low levels of concentration, therefore assays developed to measure them must be very sensitive. Enzyme linked immunosorbent assays (ELISA's) developed using manufacturers recommended end points can detect cytokines to picogram levels but the lower parts of their standard curves can be unreliable. In this study the relative merits of different substrate systems - 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 2 forms of tetramethyl benzidine (TMB), were investigated with regard to assay sensitivity. Further, a signal amplification method involving biotinylated tyramine has been used to increase the absorbance signal and thus the assay sensitivity and to achieve a sigmoidal standard curve. The amplified assay approach has been applied successfully to achieve more sensitive detection of TNF-alpha and improve the sensitivity of assays for a wide range of other cytokines. The optimised amplification method is the same for all the cytokine ELISA's performed in this work and this enables them to be performed","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"20 1-2","pages":"31-44"},"PeriodicalIF":0.0,"publicationDate":"1999-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529909349312","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21095926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 48

Differentiation between endotoxin and non-endotoxin pyrogens in human albumin solutions using an ex vivo whole blood culture assay. 人白蛋白溶液中内毒素和非内毒素热原的体外全血培养鉴别。

Journal of immunoassay Pub Date : 1999-02-01 DOI: 10.1080/01971529909349315

E J Pool, G Johaar, S James, I Petersen, P Bouic

{"title":"Differentiation between endotoxin and non-endotoxin pyrogens in human albumin solutions using an ex vivo whole blood culture assay.","authors":"E J Pool, G Johaar, S James, I Petersen, P Bouic","doi":"10.1080/01971529909349315","DOIUrl":"https://doi.org/10.1080/01971529909349315","url":null,"abstract":"Purified E.coli endotoxin, Gram negative bacteria and Gram positive bacteria induce IL-6 secretion by whole blood cultures (WBC's). Polymyxin B at concentrations greater than 2 U/ml completely inhibits IL-6 secretion caused by 10 EU/ml of endotoxin. Polymyxin B has no effect on IL-6 secretion by WBC's in the absence of endotoxin. The inhibition of endotoxin induced IL-6 secretion is Polymyxin B concentration dependent at concentrations less than 1 U/ml. IL-6 induction caused by E.coli is only partially inactivated by 8 U/ml Polymyxin B. Polymyxin B has no effect on IL-6 secretion caused by B.subtilis. Two pyrogenic batches of human serum albumin (HSA), as tested by the rabbit assay for pyrogens, were also investigated. Polymyxin B at 4 U/ml inhibits less than 40 % of IL-6 secretion caused by these pyrogenic HSA batches. All the endotoxin activity in HSA samples spiked with purified endotoxin is inhibited by Polymyxin B indicating that HSA does not protect endotoxin against Polymyxin B inhibition. These results indicate that the pyrogenicity of these HSA batches are caused by Polymyxin B inhibitable and non-inhibitable fractions. This study shows that pyrogenic substances other than endotoxin can contaminate batches of pharmaceutical products and that results obtained using the Limulus Amoebocyte Lysate (LAL) assay does not necessarily indicate the pyrogenic status of pharmaceutical products. The WBC assay for pyrogens, having a broader sensitivity range than the LAL assay, is a better indicator of the pyrogenic status of pharmaceutical products.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"20 1-2","pages":"79-89"},"PeriodicalIF":0.0,"publicationDate":"1999-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529909349315","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21095928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Standardization of smooth lipopolysaccharide preparations for use in diagnostic serological tests for bovine antibody Brucella abortus. 牛抗体流产布鲁氏菌诊断血清学试验用光滑脂多糖制剂的标准化。

Journal of immunoassay Pub Date : 1998-11-01 DOI: 10.1080/01971529808005484

K Nielsen, L Kelly, M Mallory

引用次数: 5

Development of an immunoassay for monitoring the levels of ciprofloxacin in patient samples. 一种用于监测患者样本中环丙沙星水平的免疫测定方法的发展。

Journal of immunoassay Pub Date : 1998-11-01 DOI: 10.1080/01971529808005483

G G Snitkoff, D W Grabe, R Holt, G R Bailie

引用次数: 19