Journal of immunoassay最新文献

Detection of anti-GM1 ganglioside antibodies in patients with neuropathy by a novel latex agglutination assay. 新型乳胶凝集试验检测神经病变患者抗gm1神经节苷脂抗体。

Journal of immunoassay Pub Date : 2000-11-01 DOI: 10.1080/01971520009349543

A Alaedini, N Latov

引用次数: 17

Use of dot-blot immunogold assay to identify a proliferative antigen in the initial cells of a wheat stem meristem. 利用免疫金斑点法鉴定小麦茎分生组织初始细胞中的增殖抗原。

Journal of immunoassay Pub Date : 2000-11-01 DOI: 10.1080/01971520009349545

M V Sumaroka, L A Dykman, V A Bogatyrev, N V Evseeva, I S Zaitseva, S Y Shchyogolev, A D Volodarsky

引用次数: 6

Osteoclast differentiation factor in human osteosarcoma cell line. 人骨肉瘤细胞系破骨细胞分化因子的研究。

Journal of immunoassay Pub Date : 2000-11-01 DOI: 10.1080/01971520009349540

K Kinpara, M Mogi, M Kuzushima, A Togari

引用次数: 40

A simple method of electroelution of individual protein bands from SDS polyacrylamide gels for direct study in cellular assays. 一种简单的从SDS聚丙烯酰胺凝胶中电洗脱单个蛋白带的方法，用于细胞分析的直接研究。

Journal of immunoassay Pub Date : 2000-11-01 DOI: 10.1080/01971520009349542

S Bhaskar, S Dutt, R Mukherjee

{"title":"A simple method of electroelution of individual protein bands from SDS polyacrylamide gels for direct study in cellular assays.","authors":"S Bhaskar, S Dutt, R Mukherjee","doi":"10.1080/01971520009349542","DOIUrl":"https://doi.org/10.1080/01971520009349542","url":null,"abstract":"A very simple and effective procedure which allows simultaneous electroelution of separated proteins from SDS polyacrylamide gel into small quantity of elution buffer is described. Elution parameters have been optimized for maximum possible recovery (50-60%). Protein fractions were collected in physiological buffer and an efficient removal of SDS have been obtained, thus fractions collected were suited for direct testing in cell cultures. Method was used to investigate human T-cell responses to purified secreted M tuberculosis H37Rv proteins. Eight low molecular weight (M.w. range 10 kD to 25 kD) culture filtrate proteins were purified in quantities, sufficient for immunological characterization. Lymphocyte proliferative responses and cytokine release pattern from tuberculosis patients, healthy contacts and healthy controls were studied on stimulation with purified culture filtrate proteins. Immunologically important M.tuberculosis proteins were identified by using this method. This approach should be applicable to the rapid identification and characterization of any interesting T cell antigen.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"21 4","pages":"355-75"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971520009349542","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21898374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Inflammatory activity as an indicator of water quality: the use of human whole blood cultures. 作为水质指标的炎症活动:人类全血培养的使用。

Journal of immunoassay Pub Date : 2000-11-01 DOI: 10.1080/01971520009349544

E J Pool, J H van Wyk, A J Leslie

引用次数: 15

Development of a peptide-based sandwich ELISA for human tissue prokallikrein with no cross-reactivity from mature kallikrein. 基于多肽的夹心酶联免疫吸附测定人组织原钾激肽，与成熟钾激肽无交叉反应性。

Journal of immunoassay Pub Date : 2000-11-01 DOI: 10.1080/01971520009349546

S Mauer, A Maidhof, M Kemme

{"title":"Development of a peptide-based sandwich ELISA for human tissue prokallikrein with no cross-reactivity from mature kallikrein.","authors":"S Mauer, A Maidhof, M Kemme","doi":"10.1080/01971520009349546","DOIUrl":"https://doi.org/10.1080/01971520009349546","url":null,"abstract":"Human tissue prokallikrein is the enzymatically inactive zymogen of a serine proteinase involved in the liberation of vasoactive kinin peptides, and it is supposed that an impaired prokallikrein-to-kallikrein conversion is closely related to certain hypertensive and inflammatory disorders. Progress in understanding the biological role of the proenzyme has been limited by the absence of an accurate assay for the kallikrein precursor. We describe a sandwich enzyme-linked immunosorbent assay to measure human tissue prokallikrein using monospecific anti-peptide antibodies raised against propeptide derivatives. This method could detect a minimum concentration of 60 pg/ml prokallikrein and displayed no cross-reactivity or interference with mature tissue kallikrein. The intra- and inter-assay precision varied from 8-15%, respectively, indicating a reasonable reproducibility of the method. The level of prokallikrein was defined in different human urine samples, and the corresponding dilution curves showed good linearity. The mean recovery of added zymogen was 104%. Prokallikrein immunoassay is the first reported tool for the direct and sensitive quantification of the precursor of tissue kallikrein and should facilitate the precise determination of prokallikrein levels in a variety of biological specimen.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"21 4","pages":"411-26"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971520009349546","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21898378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of blocking agents for an ELISA for LPS. 脂多糖酶联免疫吸附检测阻断剂的比较。

Journal of immunoassay Pub Date : 2000-11-01 DOI: 10.1080/01971520009349541

Z Péterfi, B Kocsis

引用次数: 51

Determination of optimal conditions for the immobilization of cells in a cell capture enzyme immunoassay (CC-EIA) by a simple Geimsa assay. 用简单的Geimsa法确定细胞捕获酶免疫测定(CC-EIA)中细胞固定的最佳条件。

Journal of immunoassay Pub Date : 2000-11-01 DOI: 10.1080/01971520009349538

M Kobayashi, K Sano, K Katsumura, K Tanaka, T Sugiyama, M Doi, M Abe, T Ikeda

引用次数: 4

Rotavirus detection using ultrasound enhanced latex agglutination and turbidimetry. 超声增强乳胶凝集和浊度法检测轮状病毒。

Journal of immunoassay Pub Date : 2000-11-01 DOI: 10.1080/01971520009349539

M A Sobanski, R W Ellis, J G Hastings

{"title":"Rotavirus detection using ultrasound enhanced latex agglutination and turbidimetry.","authors":"M A Sobanski, R W Ellis, J G Hastings","doi":"10.1080/01971520009349539","DOIUrl":"https://doi.org/10.1080/01971520009349539","url":null,"abstract":"Application of a non-cavitating ultrasonic standing wave to suspended microparticles brings the particles into close approximation and has been used previously to enhance the performance of several diagnostic agglutination tests. The sensitivity of rotavirus detection by ultrasound enhanced latex agglutination was compared with conventional test-card agglutination. Application of ultrasound gave a 32-fold improvement in the sensitivity of detection of rotavirus antigen in buffer compared with the test card method. A novel turbidimetric approach was used to measure agglutination occurring following the test-card procedure (in place of visual examination) and following exposure of commercial rotavirus latex reagents to a 4.5 MHz ultrasonic field (in place of microscopy). The sensitivity enhancement over the conventional method achievable through ultrasonic exposure was comparable whether agglutination measurements were made visually or turbidimetrically and demonstrates the potential for turbidimetry in combination with the ultrasonic method. Turbidimetry offers an alternative to visual assessment that may be more easily incorporated into automated systems.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"21 4","pages":"315-25"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971520009349539","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21897144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Precipitation and agglutination. 沉淀和凝集。

Journal of immunoassay Pub Date : 2000-05-01 DOI: 10.1080/01971520009349532

C J van Oss

引用次数: 7