Journal of immunoassay最新文献_第10页

Journal of immunoassay Pub Date : 1996-05-01 DOI: 10.1080/01971529608005787

B Ferrua, H Chakboub, C Roptin, R Garraffo, A Faraj, J Grassi, R Guedj, J P Sommadossi

{"title":"Quantitation of 3'-amino-3'-deoxythymidine (AMT), a toxic catabolite of 3'-azido-3'-deoxythymidine (AZT), by competitive ELISA.","authors":"B Ferrua, H Chakboub, C Roptin, R Garraffo, A Faraj, J Grassi, R Guedj, J P Sommadossi","doi":"10.1080/01971529608005787","DOIUrl":"https://doi.org/10.1080/01971529608005787","url":null,"abstract":"In the present study, a competitive ELISA technique was developed to specifically quantitate 3'-amino-3'-deoxythymidine (AMT), a toxic catabolite of 3'-azido-3'-deoxythymidine (AZT) detected in serum from AZT-treated patients. In order to eliminate cross-reacting AZT, serum sample was extracted with ethylacetate and then AMT was acetylated (Ac-AMT). A 5'-hemisuccinate-AMT-horseradish peroxidase conjugate was used as a tracer in the presence of anti-AMT rabbit antibodies which were raised against a 5' hemisuccinate-AMT-bovine serum albumin immunogen. Bound/free separation was achieved with an anti-rabbit IgG mouse monoclonal antibody insolubilized onto a microtiter plate. The limit of quantification of Ac-AMT was as low as 0.4 ng/ml in serum samples. This ELISA technique was applied for monitoring AMT plasma levels in patients receiving AZT therapy. The intra and inter-individual variations of the AZT/AMT plasma concentration ratios underlined the need for such a specific test in studying the formation of this toxic catabolite.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"17 2","pages":"175-93"},"PeriodicalIF":0.0,"publicationDate":"1996-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529608005787","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19716811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Enzyme immunoassay for conjugated cholic and 1 beta-hydroxycholic acids in urine of early infancy. 早期婴儿尿液中共轭胆酸和1 -羟基胆酸的酶免疫测定。

Journal of immunoassay Pub Date : 1996-05-01 DOI: 10.1080/01971529608005782

S Ikegawa, A Kinoshita, K Kido, T Murai, T Yoshimura, M Tohma

引用次数: 1

Enzyme-linked immunosorbent assay of neocarzinostatin chromophore (NCS-chr) by use of a monoclonal antibody against NCS-chr analog. 利用NCS-chr类似物单克隆抗体，酶联免疫吸附法测定新羧抑素发色团(NCS-chr)。

Journal of immunoassay Pub Date : 1996-05-01 DOI: 10.1080/01971529608005784

M Mizugaki, K Itoh, H Nakamura, N Ishida

引用次数: 0

The quantitation of coupled bead antibody by enzyme-linked immunosorbent assay. 酶联免疫吸附法定量偶联头抗体。

Journal of immunoassay Pub Date : 1996-05-01 DOI: 10.1080/01971529608005785

R Derango, J Page

引用次数: 13

An immunoradiometric assay for thymosin alpha 1. 胸腺素α 1的免疫放射测定。

Journal of immunoassay Pub Date : 1996-02-01 DOI: 10.1080/01971529608005780

H Yokoi, T Saitoh, Y Nakazawa, H Ohno

{"title":"An immunoradiometric assay for thymosin alpha 1.","authors":"H Yokoi, T Saitoh, Y Nakazawa, H Ohno","doi":"10.1080/01971529608005780","DOIUrl":"https://doi.org/10.1080/01971529608005780","url":null,"abstract":"An Immunoradiometric assay for thymosin alpha 1(1-28) was developed using a monoclonal and a polyclonal antiserum which were raised against synthetic thymosin alpha 1(1-28) and thymosin alpha 1(16-28), respectively. A monoclonal antibody, specific for the (acetylated ser1) 1-5 sequence of thymosin alpha 1, was immobilized on polystyrene beads for the solid phase, and a polyclonal antiserum specific for the 16-28 sequence was employed. This method relies on the formation of an immune complex consisting of a 125I-labelled anti-rabbit IgG goat antibody, polyclonal antiserum, thymosin alpha 1, and the solid phase monoclonal antibody. Radioactivity on the solid phase is directly proportional to the amount of thymosin alpha 1 present in the specimen. The minimal detection limit of this assay system was approximately 1.9 pg/ml. The mean values of thymosin alpha 1 in plasma of healthy subject, ranging in age from 0 to 3 years was approximately five fold higher than that of higher ages. HPLC analysis of plasma of a healthy subjects revealed a single immunoreactive form which eluted with the same retention time as that of synthetic thymosin alpha 1. This assay will be extremely useful for the measurement of thymosin alpha 1 in biologic fluids and tissues.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"17 1","pages":"85-100"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529608005780","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19891007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Comparison of 5-hydroxy-2, 3-dihydrophthalazine-1, 4-dione and luminol as co-substrates for detection of horseradish peroxidase in enhanced chemiluminescent reactions. 5-羟基- 2,3 -二氢酞嗪- 1,4 -二酮和鲁米诺共底物在增强化学发光反应中检测辣根过氧化物酶的比较。

Journal of immunoassay Pub Date : 1996-02-01 DOI: 10.1080/01971529608005779

L J Kricka, X Ji, G H Thorpe, B Edwards, J Voyta, I Bronstein

引用次数: 11

Detection of adhesion of superantigen-activated T lymphocytes to human endothelial cells by ELISA. ELISA法检测超抗原活化T淋巴细胞对人内皮细胞的粘附。

Journal of immunoassay Pub Date : 1996-02-01 DOI: 10.1080/01971529608005775

T Krakauer

引用次数: 3

Direct microtitre plate radioimmunoassay of savoxepine in unextracted plasma. 未提取血浆中沙伏塞平的直接微滴板放射免疫测定。

Journal of immunoassay Pub Date : 1996-02-01 DOI: 10.1080/01971529608005777

G Lefèvre, M Duval, L Botta, J Godbillon

引用次数: 3

Enzyme-linked immunosorbent assay of pravastatin, a HMG-CoA reductase inhibitor, in human plasma. 人血浆中HMG-CoA还原酶抑制剂普伐他汀的酶联免疫吸附测定。

Journal of immunoassay Pub Date : 1996-02-01 DOI: 10.1080/01971529608005776

S Muramatsu, W Takasaki, M Uchiyama, Y Komokata, Y Tanaka, H Takahagi

{"title":"Enzyme-linked immunosorbent assay of pravastatin, a HMG-CoA reductase inhibitor, in human plasma.","authors":"S Muramatsu, W Takasaki, M Uchiyama, Y Komokata, Y Tanaka, H Takahagi","doi":"10.1080/01971529608005776","DOIUrl":"https://doi.org/10.1080/01971529608005776","url":null,"abstract":"An enzyme-linked immunosorbent assay (ELISA) was developed for sensitive and specific determination of pravastatin (PS) sodium, a HMG-CoA reductase inhibitor. Preparation of immunogens to obtain antisera was carried out using chemically modified PS; beta-alanine derivative of PS (for ELISA-1) and 5-deoxy- PS (for ELISA-2) were linked to bovine serum albumin via its terminal carboxylic acid by the N-succinimidyl ester method, to avoid intramolecular lactonization of PS. Enzyme-labeled antigens were prepared similarly by coupling with horseradish peroxidase, and were used by homogeneous combination of antisera. The enzymic activity was determined using a microtiter plate coated with second antibody and tetramethylbenzidine as a chromogenic substrate. Both of the ELISA systems enabled the determination of PS in a range of 5 to 500 pg/well, with an IC50 of 36 to 130 pg/well. Cross-reactivties with main metabolites in plasma, which differed from PS in decaline moiety, were less than a few percent. When ELISA-1 was applied to the determination of PS in human plasma directly after dilution with the ELISA buffer, the detection limit and the intra-assay coefficient (5 ng/ml of PS) were 500 pg/ml and 4.5%, respectively. Further, ELISA-1 was validated by gas chromatography-mass spectrometry with the determination of PS in human plasma after oral administration at a dose of 10 mg/body.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"17 1","pages":"13-27"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529608005776","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19891003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Study of binding of thyroxin-conjugates to the thyroxin-binding proteins. 甲状腺素偶联物与甲状腺素结合蛋白结合的研究。

Journal of immunoassay Pub Date : 1996-02-01 DOI: 10.1080/01971529608005778

S Georgiou, I Christofidis

{"title":"Study of binding of thyroxin-conjugates to the thyroxin-binding proteins.","authors":"S Georgiou, I Christofidis","doi":"10.1080/01971529608005778","DOIUrl":"https://doi.org/10.1080/01971529608005778","url":null,"abstract":"In this work we studied the effects of the molecular weight (M.W.) of thyroxin (T4)-conjugates and the sample dilution factor on the binding potential (C) of serum T4-binding proteins for these T4-conjugates. We prepared six T4-conjugates with great difference in molecular weight with proteins such as IgG, apoferritin, ferritin, transferrin, and thyroglobulin using p-benzoquinone as bifunctional reagent. The conjugates prepared were characterized in terms of their M.W., the molar ratio of T4 to the carrier protein, and their affinity to bind with the anti-Tr antibody. The binding potential values of serum T4-binding proteins for the T4-conjugates were determined, following appropriate mathematical interpretation of the results, obtained through an assay system containing 125I-labeled conjugated tracers, anti-T4 antibody in great excess compared with the concentration of the tracers, and increasing concentration of T4-binding proteins. We conclude that the M.W. of the conjugates is a parameter which significantly influences the binding of a conjugate to the T4-binding proteins. Additionally, for conjugates of very high M.W. (> 650,000), zero C values were obtained using 20-fold sample dilution in the final incubation mixture.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"17 1","pages":"47-66"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529608005778","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19891005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3