{"title":"A comparison of performances of four enzymes used in ELISA with special reference to beta-lactamase.","authors":"M I Khatkhatay, M Desai","doi":"10.1080/01971529909349349","DOIUrl":null,"url":null,"abstract":"<p><p>Horse radish peroxidase, alkaline phaosphatase and beta-D-galactosidase are widely used as labels in the development of enzyme immunoassays (EIAs). Enzyme beta-lactamase, though introduced as a label in late seventies has not yet become very popular inspite of having the necessary features of an enzyme to be used in EIAs. The present article reviews assays developed with this enzyme, highlights its salient features and brings out an argument in favour of its wide spread use in EIAs.</p>","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"20 3","pages":"151-83"},"PeriodicalIF":0.0000,"publicationDate":"1999-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529909349349","citationCount":"18","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of immunoassay","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/01971529909349349","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 18
Abstract
Horse radish peroxidase, alkaline phaosphatase and beta-D-galactosidase are widely used as labels in the development of enzyme immunoassays (EIAs). Enzyme beta-lactamase, though introduced as a label in late seventies has not yet become very popular inspite of having the necessary features of an enzyme to be used in EIAs. The present article reviews assays developed with this enzyme, highlights its salient features and brings out an argument in favour of its wide spread use in EIAs.
马萝卜过氧化物酶、碱性磷酸酶和β - d -半乳糖苷酶被广泛用作酶免疫测定(EIAs)的标记物。酶-内酰胺酶虽然在七十年代末作为一种标签引入，但尽管具有用于环境影响评价的酶的必要特征，但尚未变得非常流行。本文回顾了用这种酶开发的分析方法，突出了它的显著特征，并提出了赞成它在环境影响评价中广泛使用的论点。
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