Journal of immunoassay & immunochemistry最新文献_第9页

Association between inflammatory cytokines/chemokines, clinical laboratory parameters, disease severity and in-hospital mortality in critical and mild COVID-19 patients without comorbidities or immune-mediated diseases. 无合并症或免疫介导性疾病的COVID-19危重和轻度患者炎症因子/趋化因子、临床实验室参数、疾病严重程度与住院死亡率的关系

Journal of immunoassay & immunochemistry Pub Date : 2023-01-02 DOI: 10.1080/15321819.2022.2104124

Muaawia Hamza, Muhanad Alhujaily, Bandar Alosaimi, Karim El Bakkouri, Mohammed S AlDughaim, Mona Alonazi, Mona Awad Alanazi, Basma Abbass, Abdulsalam Alshehri, Samia T Al-Shouli, Wael Alturaiki, Maaweya Awadalla

{"title":"Association between inflammatory cytokines/chemokines, clinical laboratory parameters, disease severity and in-hospital mortality in critical and mild COVID-19 patients without comorbidities or immune-mediated diseases.","authors":"Muaawia Hamza, Muhanad Alhujaily, Bandar Alosaimi, Karim El Bakkouri, Mohammed S AlDughaim, Mona Alonazi, Mona Awad Alanazi, Basma Abbass, Abdulsalam Alshehri, Samia T Al-Shouli, Wael Alturaiki, Maaweya Awadalla","doi":"10.1080/15321819.2022.2104124","DOIUrl":"https://doi.org/10.1080/15321819.2022.2104124","url":null,"abstract":"There are limited data on inflammatory cytokines and chemokines; the humoral immune response; and main clinical laboratory parameters as indicators for disease severity and mortality in patients with critical and mild COVID-19 without comorbidities or immune-mediated diseases in Saudi Arabia. We determined the expression levels of major proinflammatory cytokines and chemokines; C-reactive protein (CRP); procalcitonin; SARS-CoV-2 IgM antibody and twenty-two clinical laboratory parameters and assessed their usefulness as indicators of disease severity and in-hospital death. Our results showed a significant increase in the expression levels of SARS-CoV-2 IgM antibody; IL1-β; IL-6; IL-8; TNF-α and CRP in critical COVID-19 patients; neutrophil count; urea; creatinine and troponin were also increased. The elevation of these biomarkers was significantly associated and positively correlated with in-hospital death in critical COVID-19 patients. Our results suggest that the levels of IL1-β; IL-6; IL-8; TNF-α; and CRP; neutrophil count; urea; creatinine; and troponin could be used to predict disease severity in COVID-19 patients without comorbidities or immune-mediated diseases. These inflammatory mediators could be used as predictive early biomarkers of COVID-19 disease deterioration; shock and death among COVID-19 patients without comorbidities or immune-mediated diseases.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"44 1","pages":"13-30"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10496478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

C-reactive protein and uric acid roles in distinguishing ST-segment elevation myocardial infarction from non-ST-elevation acute coronary syndrome. c反应蛋白和尿酸在区分st段抬高型心肌梗死和非st段抬高型急性冠状动脉综合征中的作用

Journal of immunoassay & immunochemistry Pub Date : 2023-01-02 DOI: 10.1080/15321819.2022.2119866

Batool Zamani, Allahyar Golabchi, Nasrin Ghadakkar, Hossein Motedayyen

{"title":"C-reactive protein and uric acid roles in distinguishing ST-segment elevation myocardial infarction from non-ST-elevation acute coronary syndrome.","authors":"Batool Zamani, Allahyar Golabchi, Nasrin Ghadakkar, Hossein Motedayyen","doi":"10.1080/15321819.2022.2119866","DOIUrl":"https://doi.org/10.1080/15321819.2022.2119866","url":null,"abstract":"Acute coronary syndrome (ACS) is defined as a range of conditions which the blood flow to the heart was reduced or stopped. This disorder is correlated to a systemic inflammatory response and some biochemical factors. Therefore, the aim of this study was investigations of serum C-reactive protein (CRP) and uric acid levels in ST-segment elevation myocardial infarction (STEMI) and non-ST-elevation ACS (NSTE ACS), as common subtypes of ACS. Patients with ACS (n = 140) were assessed with coronary arteriography and divided into STEMI and NSTE ACS groups. The serum levels of hs-CRP and uric acid were investigated using a routine clinical chemistry analyzer. Patients with STEMI showed a significant increase in uric acid level compared to those with NSTE ACS (P < .0001). Other data indicated that hs-CRP level in patients with STEMI was significantly higher than individuals with NSTE ACS (P < .0001). Modeling analysis revealed that the increased levels of acid uric and hs-CRP in patients with STEMI were independent of the effects of age, gender, background diseases, and familial history (P < .001). The current study provides further evidence to indicate that hs-CRP and uric acid may be considered as biofactors for comparing STEMI from NSTE ACS and determining disease outcome.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"44 1","pages":"66-75"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10520841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of circulating miR-148a and miR-126 with autoantibodies as biomarkers of lupus nephritis in patients with SLE. 循环miR-148a和miR-126与自身抗体作为狼疮肾炎患者生物标志物的比较

Journal of immunoassay & immunochemistry Pub Date : 2022-11-02 Epub Date: 2022-08-08 DOI: 10.1080/15321819.2022.2099225

Frouzan Omidi, Majid Khoshmirsafa, Nahid Kianmehr, Fatemeh Faraji, Ali Delbandi, Farhad Seif, Mehdi Shekarabi

{"title":"Comparison of circulating miR-148a and miR-126 with autoantibodies as biomarkers of lupus nephritis in patients with SLE.","authors":"Frouzan Omidi, Majid Khoshmirsafa, Nahid Kianmehr, Fatemeh Faraji, Ali Delbandi, Farhad Seif, Mehdi Shekarabi","doi":"10.1080/15321819.2022.2099225","DOIUrl":"https://doi.org/10.1080/15321819.2022.2099225","url":null,"abstract":"Lupus nephritis (LN) is the main manifestation of systemic Lupus Erythematosus (SLE). MicroRNAs (miRNAs) and autoantibodies could be suitable candidate biomarkers of LN. This study evaluates the expression of circulating miR-148a and miR-126 along with anti-dsDNA, anti-C1q, and anti-C3b autoantibodies in SLE patients with LN (SLE + LN). 30 women with SLE, 30 women with SLE + LN, and 25 women as healthy controls (HCs) were enrolled in this study. The plasma expression of selected miRNAs was evaluated by real-time PCR. The serum level of anti-dsDNA, C1q, and C3b antibodies was measured by the ELISA. The expression of miR-148a was significantly increased in SLE and SLE+LN groups compared with the control group. No significant difference was found in the expression of miR-126 among the groups. The frequency of autoantibodies was significantly higher in the SLE + LN group than SLE. The Higher levels of circulating miR-148a in the SLE samples compared with the HCs suggest that this miRNA could be a reliable biomarker for SLE patients (with or without LN). Also, autoantibodies against dsDNA, C1q, and, C3 could be used for the prediction of SLE nephritis, independently. However, further studies are needed to confirm these findings.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"634-647"},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40609611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical and clinical impacts of miR-150 and miR-21 expression levels in diffuse large B cell lymphoma. miR-150和miR-21表达水平对弥漫性大B细胞淋巴瘤的生化和临床影响

Journal of immunoassay & immunochemistry Pub Date : 2022-11-02 Epub Date: 2022-08-01 DOI: 10.1080/15321819.2022.2103431

Elsayed Saber Abou Elnour, Ibrahim El Tantawy El Sayed, Hamed Mohamed Abd Elbary, Ahmed Sohaib, Shaimaa Amin Mohammed Atia, Shaimaa El Sayed Ramadan Genena

{"title":"Biochemical and clinical impacts of miR-150 and miR-21 expression levels in diffuse large B cell lymphoma.","authors":"Elsayed Saber Abou Elnour, Ibrahim El Tantawy El Sayed, Hamed Mohamed Abd Elbary, Ahmed Sohaib, Shaimaa Amin Mohammed Atia, Shaimaa El Sayed Ramadan Genena","doi":"10.1080/15321819.2022.2103431","DOIUrl":"https://doi.org/10.1080/15321819.2022.2103431","url":null,"abstract":"Identification of biomarkers is crucial in guiding the treatment decision and improving the future outcomes of DLBCL. The aim of the current study is to detect the biochemical and clinical impacts of miR-150 and miR-21 expression levels in DLBCL. Quantification of serum miR-150 and miR-21 expression levels by real-time PCR after micro-RNA extraction and RT-PCR. At a cutoff point of 2.3 for miR-21, the sensitivity, specificity, positive predictive, and negative predictive values for diagnosis of DLBCL were 98%, 90%, 90.7%, and 97.8%, respectively. At cut-off point (≤19.12) the sensitivity, specificity, the positive predictive and negative predictive values of miR-21 to discriminate stage IV vs stage II DLBCL patients were 68.42%, 80%, 86.7%%,and 57.1%, respectively. Serum miR-150 and serum miR-21 can be used as diagnostic markers for DLBCL patients, but miR-21 is more sensitive than miR-150. Serum miR-21 can be used as prognostic marker for DLBCL patients. It was more sensitive and more specific than miR-150.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"648-664"},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40674819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of leptin gene polymorphism and leptin serum level in alopecia areata patients. 斑秃患者瘦素基因多态性及血清瘦素水平的研究。

Journal of immunoassay & immunochemistry Pub Date : 2022-11-02 Epub Date: 2022-06-20 DOI: 10.1080/15321819.2022.2088294

Heba Bazid, Mostafa Hammam, Mohammed Mostafa, Yasmine Gamal, Eman M Abd El Gayed

{"title":"Study of leptin gene polymorphism and leptin serum level in alopecia areata patients.","authors":"Heba Bazid, Mostafa Hammam, Mohammed Mostafa, Yasmine Gamal, Eman M Abd El Gayed","doi":"10.1080/15321819.2022.2088294","DOIUrl":"https://doi.org/10.1080/15321819.2022.2088294","url":null,"abstract":"Leptin, produced by adipocytes, regulates metabolism, hunger, and immune response. The inflammatory role of leptin has been linked to autoimmune diseases. To assess leptin gene polymorphism and serum level in alopecia areata and their relation to metabolic syndrome (MS). This case-control study was conducted on 100 alopecia areata patients (50 with MS and 50 without MS) and 50 age- and gender-matched controls. Leptin gene polymorphism and serum level were assessed through the use of PCR and ELISA, respectively. GG genotype was the highest in AA with MS (54%), lower in AA without MS (42%), and the lowest in controls (20%). G allele was more expressed in cases, than in controls (P < .001). The serum leptin level was the highest in AA with MS, lower in AA without MS, and the lowest in controls (P value = 0.001). Leptin level was significantly higher in GG polymorphism than AG and AA. Leptin gene polymorphism (GG genotype) and serum level appear to play a significant role in AA. Absent difference regarding leptin gene polymorphism and MS might indicate a separate inflammatory role of leptin or the future risk of MS development in AA patients.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"605-617"},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40122027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fibroblast activation protein (FAP) expression in CK5/6 expressed (Basal subtype) & CK20 expressed (Luminal subtype) urothelial bladder carcinoma: an immunohistochemical study. 成纤维细胞激活蛋白(FAP)在CK5/6表达(基底亚型)和CK20表达(腔型亚型)尿路上皮膀胱癌中的表达:免疫组织化学研究

Journal of immunoassay & immunochemistry Pub Date : 2022-11-02 Epub Date: 2022-07-04 DOI: 10.1080/15321819.2022.2095208

Aiat Shaban Hemida, Hayam Abd El Samae Aiad, Nourhan Anwar Hassan, Dalia Rifaat Al Sharaky

{"title":"Fibroblast activation protein (FAP) expression in CK5/6 expressed (Basal subtype) & CK20 expressed (Luminal subtype) urothelial bladder carcinoma: an immunohistochemical study.","authors":"Aiat Shaban Hemida, Hayam Abd El Samae Aiad, Nourhan Anwar Hassan, Dalia Rifaat Al Sharaky","doi":"10.1080/15321819.2022.2095208","DOIUrl":"https://doi.org/10.1080/15321819.2022.2095208","url":null,"abstract":"Urinary bladder cancer incidence varies all over the world. Egypt displays high incidence rates. Molecular subtyping helps risk stratification and personalized treatment. Cancer-associated fibroblasts (CAFs) in the tumor microenvironment may provoke tumor-promotion or tumor suppression. Fibroblast activation protein (FAP) is a marker of CAFs, suggested to accelerate tumor progression in various cancers. In urothelial carcinoma, investigations regarding impact of FAP expression on prognosis are needed. This work aims to study impact of FAP expression in urothelial carcinoma and find its relation to CK 5/6 (basal) expressed and CK 20 (luminal) expressed immunohistochemical markers. This retrospective study included 70 urothelial carcinoma specimens. Immunohistochemistry was performed and results were analyzed. FAP was expressed in 67.1% of cases and showed significant association with advanced tumor stage, muscle invasion, mitoses in tumor cells and stratified groups; as 73.9% of FAP positive cases were of Ck5/6+/Ck20- (basal subtype). All studied parameters did not show significant association with patient's overall survival. In conclusion, FAP could have a role in modulating tumor microenvironment and promoting tumor invasion. FAP is correlated with basal subtype of urothelial carcinoma, which may be an indicator of tumor aggressiveness. FAP antagonists may be helpful in preventing tumor progression.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"618-633"},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40480509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances of lateral flow immunoassay components as "point of need". 横向流动免疫测定组分作为“需要点”的最新进展。

Journal of immunoassay & immunochemistry Pub Date : 2022-11-02 Epub Date: 2022-09-15 DOI: 10.1080/15321819.2022.2122063

Susraba Chatterjee, Sumi Mukhopadhyay

{"title":"Recent advances of lateral flow immunoassay components as \"point of need\".","authors":"Susraba Chatterjee, Sumi Mukhopadhyay","doi":"10.1080/15321819.2022.2122063","DOIUrl":"https://doi.org/10.1080/15321819.2022.2122063","url":null,"abstract":"Lateral flow immunoassay is the leading Point of Care test and is becoming increasingly essential for its versatile properties. The attraction of lateral flow assay (LFA) has reached its prime position during recent SARS-CoV-2 pandemic and Ebola, Zika epidemics in third world countries where primary screening of the disease and financial issues are very important. During the last decade traditional methodology of LFA was limited to visual detection and qualitative assessment only. However, recently researchers are focusing on the development and improvement of this tool to enhance its specificity, assessment power (quantitative) to make it an alternative to traditional lab-based technology. Modifying working principle and instrumentation, combination of different modern molecular techniques such as Reverse transcription loop mediated isothermal amplification (RT-LAMP), Clustered regularly inter-spaced short palindromic repeat (CRISPR-Cas), Recombinase amplification polymerase (RPA), also association of image-based software, involvement of nanotechnology, implementation of LFA ruler have established authenticity and ultra-specific detection level. These leading immunochromatographic techniques offer simultaneous detection of different analytes from a single sample unit into one multiplex strip and provide the necessary information. This review is a foremost attempt to encompass recent advances of lateral flow assays in combination with molecular biology techniques along with improvements of assay components for improved diagnostic sensitivity and specificity. Some infectious disease diagnosis by LFA with its reporter and low detection limit have also been mentioned in this review.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"579-604"},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40358186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Serological evaluation of Newcastle disease protection among broilers at a live bird market in Kano, Northwest Nigeria, and its epidemiological significance. 尼日利亚西北部卡诺活禽市场肉鸡新城疫防护血清学评价及其流行病学意义

Journal of immunoassay & immunochemistry Pub Date : 2022-09-03 Epub Date: 2022-02-13 DOI: 10.1080/15321819.2022.2029744

Olubukola O Funsho-Sanni, Elijah Ella, Olufunsho Samuel Sanni, Helen Inabo, Sodangi Abdulkarim Luka, RoseMary Eleyi Ameh

{"title":"Serological evaluation of Newcastle disease protection among broilers at a live bird market in Kano, Northwest Nigeria, and its epidemiological significance.","authors":"Olubukola O Funsho-Sanni, Elijah Ella, Olufunsho Samuel Sanni, Helen Inabo, Sodangi Abdulkarim Luka, RoseMary Eleyi Ameh","doi":"10.1080/15321819.2022.2029744","DOIUrl":"https://doi.org/10.1080/15321819.2022.2029744","url":null,"abstract":"Vaccination is a tool of Newcastle disease (ND) control among broilers. This study aimed at determining the immunity status of sampled broilers against ND at a live bird market in Kano, Northwest Nigeria, and its epidemiological implication. A cross-sectional study of antibodies against Newcastle disease virus (NDV) was carried out among broiler chicken in a live bird market in Kano State, Northwest Nigeria. A total of 471 samples was tested successfully. NDV antibody titer was assayed using hemagglutination-inhibition test (HI) and ND indirect enzyme-linked immunosorbent assay (ELISA). Serological levels of NDV antibodies were 67.9% (ELISA) and 78.1% (HI). Also, 67 (20.9%) samples tested positive for ELISA but negative for HI, whereas 115 (31.3%) samples tested negative for ELISA but positive for HI. There is strong association between the immune status obtained from both tests (P < .05), significant difference exists between the immune titer obtained from both tests (P < .05). Protective antibody titer among the test subjects suggests individual protection against virulent NDV (vNDV) strain; however, protective levels ≥85% that confers herd immunity were not attained. This report emphasizes the need for farmers to be more compliant to ND vaccination schedule and best practices in their poultry farm to enhance ND control in Live Bird Markets (LBMs).","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"43 5","pages":"534-545"},"PeriodicalIF":0.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39778534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Molasses, Antox® and EN-FLORAX® decreased antibody decay rate and enhanced response to a very virulent infectious bursal disease virus and Newcastle disease vaccine La Sota in ISA Brown chicks. 糖浆、Antox®和EN-FLORAX®降低了ISA Brown鸡的抗体衰减率，增强了对非常强毒的传染性法氏囊病病毒和新城疫病疫苗La Sota的反应。

Journal of immunoassay & immunochemistry Pub Date : 2022-09-03 Epub Date: 2022-02-13 DOI: 10.1080/15321819.2022.2036188

Aliyu Andamin, Paul Abdu, Ochuko Orakpoghenor, Talatu Markus, Sunday Oladele, Felix Akade, Tagang Aluwong

{"title":"Molasses, Antox® and EN-FLORAX® decreased antibody decay rate and enhanced response to a very virulent infectious bursal disease virus and Newcastle disease vaccine La Sota in ISA Brown chicks.","authors":"Aliyu Andamin, Paul Abdu, Ochuko Orakpoghenor, Talatu Markus, Sunday Oladele, Felix Akade, Tagang Aluwong","doi":"10.1080/15321819.2022.2036188","DOIUrl":"https://doi.org/10.1080/15321819.2022.2036188","url":null,"abstract":"This study evaluated the effects of molasses, Antox® and EN-FLORAX® on antibody (Ab) decay and response to a very virulent IBD virus (vvIBDV) and ND vaccine La Sota (NDVLS) in ISA Brown chicks. Five groups, (A, B, C, D and E) of 50 chicks each were used for the study. Groups A, B and C were supplemented with molasses, Antox® and EN-FLORAX®, respectively, orally from 1 to 49 days, and inoculated with a vvIBDV at 28 days of age. Groups D, and E were positive, and negative controls, respectively. At 35 days of age, all groups were vaccinated with NDVLS. Antibody (Ab) titers to vvIBDV, and NDV, were determined by indirect enzyme linked immunosorbent assay (I-ELISA), and hemagglutination-inhibition (HI) tests, respectively. Results revealed significantly (P < .05) decreased Ab decay rates in supplemented groups (A, B, and C) compared to controls (D and E) up to day 28. There were significantly (P < .05) higher mean IBDV and ND HI Ab titers in supplemented groups compared to D with the highest in A up to day 49. Molasses, Antox®, and EN-FLORAX® decreased rate of Ab decay, elicited stronger Ab response against vvIBDV and production of protective NDVLS HI Ab titers.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"43 5","pages":"546-556"},"PeriodicalIF":0.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39916426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sero-detection of antibodies to Avian metaavulavirus 2 in peri-domestic birds, Nigeria. 尼日利亚家禽中禽变avulavirus 2抗体的血清检测

Journal of immunoassay & immunochemistry Pub Date : 2022-09-03 Epub Date: 2022-01-07 DOI: 10.1080/15321819.2021.2022690

Bitrus Inuwa, Yakubu Joel Atuman, Clement Adebajo Meseko, Ismaila Shittu

{"title":"Sero-detection of antibodies to Avian metaavulavirus 2 in peri-domestic birds, Nigeria.","authors":"Bitrus Inuwa, Yakubu Joel Atuman, Clement Adebajo Meseko, Ismaila Shittu","doi":"10.1080/15321819.2021.2022690","DOIUrl":"https://doi.org/10.1080/15321819.2021.2022690","url":null,"abstract":"Avian metaavulavirus 2 (AMAV-2) previously known as the avian paramyxovirus-2 causes mild to severe respiratory disease, reduced hatchability and infertility of eggs, including increase in white-shelled eggs in chickens and Turkey breeders. When exacerbated by secondary pathogens and environmental stresses, infection is more severe leading to significant economic losses. This study was conducted to determine, if any, the presence of antibodies to Avian metaavulavirus 2 (AMAV-2) in peri-domestic birds in Bauchi State, Nigeria. In all, one hundred sera samples from pigeons (n = 10) and doves (n = 90 were collected in Bauchi, Nigeria. Based on hemagglutination-inhibition (HI) test, overall seroprevalence of 27.0% (27/100) was recorded. In pigeon, the seroprevalence was 80.0% while 21.1% was recorded for dove with HI antibody titers ranging from 3log2 to 8log2. There was statistical significance obtained between dove and pigeon sera tested (p < .05). Until now and to the best of our knowledge, there are no reports on AMAV-2 in poultry or wild birds in Nigeria. This study, thus, provides preliminary information on AMAV-2 seroprevalence in Nigerian peri-domestic birds. The need to conduct further studies in other avian species and wild birds in Nigeria is highlighted.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"43 5","pages":"526-533"},"PeriodicalIF":0.0,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39655536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0