Journal of immunoassay & immunochemistry最新文献_第8页

Prevalence and spatiotemporal distribution of rotavirus diarrhea among children younger than five years old in Lagos, Nigeria. 尼日利亚拉各斯5岁以下儿童轮状病毒腹泻的患病率和时空分布

Journal of immunoassay & immunochemistry Pub Date : 2023-03-04 DOI: 10.1080/15321819.2022.2159430

Ebelechukwu Eugenia Afocha, Bamidele Abiodun Iwalokun, Mopelola Anotu Deji-Agboola, Babatunde Ayorinde James, Taiwo Abayomi Banjo, Festus Adu, Oliver Chukwujekwu Ezechi, Richard Adegbola, Babatunde Lawal Salako

{"title":"Prevalence and spatiotemporal distribution of rotavirus diarrhea among children younger than five years old in Lagos, Nigeria.","authors":"Ebelechukwu Eugenia Afocha, Bamidele Abiodun Iwalokun, Mopelola Anotu Deji-Agboola, Babatunde Ayorinde James, Taiwo Abayomi Banjo, Festus Adu, Oliver Chukwujekwu Ezechi, Richard Adegbola, Babatunde Lawal Salako","doi":"10.1080/15321819.2022.2159430","DOIUrl":"https://doi.org/10.1080/15321819.2022.2159430","url":null,"abstract":"Data on spatiotemporal distribution of rotavirus diarrhea are limited in many endemic settings. This study determined the prevalence and seasonal distribution of rotavirus among Nigerian children with diarrhea. Here, a total of 406 fecal samples were collected from patients attending six health facilities in Lagos between January - December 2019. Socio-demographic data of each enrolled child were collected. Rotavirus VP6 antigen was detected by enzyme-linked immunoassay (ELISA) and confirmation by VP7 gene detection by reverse transcription polymerase-chain reaction. The overall rotavirus diarrhea prevalence was 16.3% by ELISA with children above 2 years having 29.2% of this prevalence and higher occurrence in females (59.1%) than males (40.9%) (P < .05). Rotavirus diarrhea diagnosis using RT-PCR showed 100% concordance with ELISA. Cases of rotavirus diarrhea were detected from March to July and from September to November with the highest number of cases detected in May and June (22.7% each), followed by July (21.2%). The prevalence of rotavirus diarrhea remains high in Lagos with an emerging higher disease activity in children above 2. A different rotavirus transmission dynamics compared to previous studies from Nigeria and other African countries was found. VP6 ELISA may reliably be used for continuous rotavirus surveillance in Nigeria.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"44 2","pages":"117-132"},"PeriodicalIF":0.0,"publicationDate":"2023-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10841464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microarray expression results of VEGF, YAP1 and PTEN immunostains in preeclampsia cases. VEGF、YAP1和PTEN免疫染色在子痫前期的微阵列表达结果。

Journal of immunoassay & immunochemistry Pub Date : 2023-03-04 DOI: 10.1080/15321819.2023.2182219

Ayhan Atigan, Yeliz Arman Karakaya, Derya Kiliç, Omer Tolga Guler

{"title":"Microarray expression results of VEGF, YAP1 and PTEN immunostains in preeclampsia cases.","authors":"Ayhan Atigan, Yeliz Arman Karakaya, Derya Kiliç, Omer Tolga Guler","doi":"10.1080/15321819.2023.2182219","DOIUrl":"https://doi.org/10.1080/15321819.2023.2182219","url":null,"abstract":"We aimed to evaluate the expression of YAP1, PTEN, VEGF in the placentas of patients with preeclampsia and placentas of healthy pregnant women for trophoblast invasion, which is similar to cancer etiopathogenesis. The placentas of 70 women who gave birth, including 30 preeclampsia and 40 healthy controls, were evaluated. YAP1, PTEN and VEGF immunohistochemical staining were performed using the microarray method on placental tissue. The mean ± standard deviation for YAP1, PTEN and VEGF intensity were; 1.57 ± 0.71,2.59 ± 0.80, 1.61 ± 0.59, respectively. PTEN intensity was statistically significantly lower in the preeclampsia group than in the control group (2.37 ± 0.99 vs 2.75 ± 0.58, p = .049). There was no difference between the groups in terms of YAP1 and VEGF staining (p > .05). The etiopathogenesis of preeclampsia is still unclear. However, since trophoblast invasion and endothelial repair have similar aspects with cancer mechanisms, both preeclampsia and cancer studies are progressing by supporting each other. Our study is a prototype study showing that large-participation studies can be carried out easily by using the microarray method as an economic model.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"44 2","pages":"204-212"},"PeriodicalIF":0.0,"publicationDate":"2023-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10866098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atypical serologic profiles of hepatitis B virus infection across clinical cohorts of patients in Southwestern Nigeria. 尼日利亚西南部患者临床队列中乙型肝炎病毒感染的非典型血清学概况

Journal of immunoassay & immunochemistry Pub Date : 2023-03-04 DOI: 10.1080/15321819.2023.2168556

Oluwadamilola Gideon Osasona, Tosin Oguntoye, Philomena Eromon, Lukman Abdulkareem, Abiola Opeyemi Arowosaye, Olumuyiwa Elijah Ariyo, Uwem Etop George, Musa Yusuf, Olubusuyi Moses Adewumi, Christian Happi, Onikepe Abiola Folarin

{"title":"Atypical serologic profiles of hepatitis B virus infection across clinical cohorts of patients in Southwestern Nigeria.","authors":"Oluwadamilola Gideon Osasona, Tosin Oguntoye, Philomena Eromon, Lukman Abdulkareem, Abiola Opeyemi Arowosaye, Olumuyiwa Elijah Ariyo, Uwem Etop George, Musa Yusuf, Olubusuyi Moses Adewumi, Christian Happi, Onikepe Abiola Folarin","doi":"10.1080/15321819.2023.2168556","DOIUrl":"https://doi.org/10.1080/15321819.2023.2168556","url":null,"abstract":"Hepatitis B virus (HBV) infection follows a natural course of events predicted by a dynamic interaction between viral antigen and the host immune system, which forms the basis for HBV serological diagnosis. These interactions may deviate from the typical serologic patterns. This study investigates the types of atypical HBV serologic profiles (AHBSP) across clinical cohorts of patients with HBV infection in southwestern Nigeria. This is a cross-sectional, hospital-based, multi-centered study. Patients' sera were analyzed for HBsAg, anti-HBs, HBeAg, anti-HBe, anti-HBc IgM, and anti-HBc IgG by ELISA from 279 study participants attending selected gastroenterology clinics between August 2019 and December 2020. The prevalence of atypical HBV serologic profiles was 27% (n = 76). The mean age of patients was 35.7 ± 11.2 years. The gender distribution involved 183 females (65.6%) and 96 males (34.4%). Across clinical cohorts of patients with atypical serologic profiles, HBeAg Negative, anti-HBe positive with detectable HBV DNA had the highest prevalence of 21% followed by isolated anti-HBc antibody positive, HBsAg negative and detectable HBV DNA, 5%. The atypical serologic profiles, HBeAg positive, HBsAg negative with detectable HBV DNA and concurrent anti-HBs with HBsAg, had the lowest prevalence, 0.4%, respectively. This study identified the considerable presence of atypical HBV serologic profiles across clinical cohorts of HBV infection in southwestern Nigeria.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"44 2","pages":"176-191"},"PeriodicalIF":0.0,"publicationDate":"2023-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10845918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Seroprevalence, trends, and risk factors of hepatitis B and C among family replacement blood donors; a 7-year retrospective study at Sunyani Municipal Hospital, Ghana. 家庭替代献血者乙型和丙型肝炎的血清阳性率、趋势和危险因素;在加纳Sunyani市立医院进行的为期7年的回顾性研究。

Journal of immunoassay & immunochemistry Pub Date : 2023-03-04 DOI: 10.1080/15321819.2023.2168555

Felix Osei-Boakye, Charles Nkansah, Samuel Kwasi Appiah, Charles Angnataa Derigubah, Kofi Mensah, Abraham Azumah Apandago, Vida Animah Boateng, Obed Gadufia Norsi, Dominic Kogh-Nuu

{"title":"Seroprevalence, trends, and risk factors of hepatitis B and C among family replacement blood donors; a 7-year retrospective study at Sunyani Municipal Hospital, Ghana.","authors":"Felix Osei-Boakye, Charles Nkansah, Samuel Kwasi Appiah, Charles Angnataa Derigubah, Kofi Mensah, Abraham Azumah Apandago, Vida Animah Boateng, Obed Gadufia Norsi, Dominic Kogh-Nuu","doi":"10.1080/15321819.2023.2168555","DOIUrl":"https://doi.org/10.1080/15321819.2023.2168555","url":null,"abstract":"Hepatitis B and C cause chronic infections which develop into liver-related sequelae, like cirrhosis and liver carcinoma. This study determined the seroprevalence, trends, and risk factors of HBV and HCV among family replacement donors. A retrospective review of primary data on blood donors screened between January 2015 and December 2021 was conducted at Sunyani Municipal Hospital. The data were assessed for seroprevalence, trends, and odds ratios using SPSS. Of 6847 donors, the majority were males (88.1% [6033]), ≤24 years (27.4% [1874]), O blood type (69.8% [4776]), and Rh-positive (89.9% [6154]). The seroprevalences of HBV and HCV were 3.2% and 1.9%, respectively, with more males infected with HBV and HCV (3.4% vs 2.0%). Males were 2.842 times (p = .001) and 2.399 times (p = .025) more susceptible than females to HBV and HCV, respectively. In the rainy season, donors were 1.489 times (p = .041) more susceptible to HCV. HBV and HCV seroprevalence declined over the period (slope: -0.5464, p ≤ .001 vs slope: -0.6179, p ≤ .001). Male gender and rainy season were significant determinants of both infections. The seroprevalence of HBV was higher than HCV despite the significant decline in both infections. We, recommend health authorities intensify health education among males and during the rainy season.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"44 2","pages":"162-175"},"PeriodicalIF":0.0,"publicationDate":"2023-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9096760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Assesement of serum Sfrp5/Wnt-5a level and its utility in the risk stratification of treatment naïve patients with metabolic syndrome. 血清srp5 /Wnt-5a水平的评估及其在治疗naïve代谢综合征患者风险分层中的应用

Journal of immunoassay & immunochemistry Pub Date : 2023-01-02 DOI: 10.1080/15321819.2022.2104125

Suraj Singh Yadav, Sartaj Hussain, Pradeep Dwivedi, Sanjay Khattri, Kamal Kumar Sawlani, Kauser Usman

{"title":"Assesement of serum Sfrp5/Wnt-5a level and its utility in the risk stratification of treatment naïve patients with metabolic syndrome.","authors":"Suraj Singh Yadav, Sartaj Hussain, Pradeep Dwivedi, Sanjay Khattri, Kamal Kumar Sawlani, Kauser Usman","doi":"10.1080/15321819.2022.2104125","DOIUrl":"https://doi.org/10.1080/15321819.2022.2104125","url":null,"abstract":"Our study focused on investigating the clinical significance of serum Sfrp5/Wnt-5a levels as a risk marker in metabolic syndrome (MetS). The study involved a total of 107 treatment-naive MetS cases and 100 controls with similar age and sex belonging to northern India. The profiling of clinical, biochemical, and anthropometric variables was done. ELISA methods were employed for serum cytokine estimation. Serum Sfrp5 was inversely correlated with BMI, WC, SBP, DBP, FPG, TG, fasting insulin level, and HOMA-IR in both males and females. The best cutoff value for Sfrp5 to predict MetS in males was ≤40.48 ng/ml (sensitivity 53.70% and specificity 90.48%), while in female, it was ≤66.67 ng/ml (sensitivity 98.11% and specificity 34.48%). MetS occurrence decreased with increasing concentration of Sfrp5 with an odds ratio (OR) of 0.95 (95% CI = 0.92-0.98, P < .001) in male and 0.93 (95% CI = 0.91-0.97, P < .001) in female. Quartile analysis revealed that odds of MetS significantly decreased in quartile 4 vs. 1, 0.06 (95% CI = 0.01-0.25), P = .001 and 0.13 (95% CI = 0.04-0.44), P = .001, respectively, in male and female. The inverse association of serum concentration of Sfrp5 with MetS might have a useful addition to the available risk marker as well as a therapeutic target for MetS.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"44 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10858528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A case of racemiform trichoblastoma in a dog. 犬外消形毛母细胞瘤1例。

Journal of immunoassay & immunochemistry Pub Date : 2023-01-02 DOI: 10.1080/15321819.2022.2137809

Samson Eneojo Abalaka, Barde Angulu Thomas, Zakariya Audu, Sunday Augustine Ejeh, Nuhu Abdulazeez Sani, Oremeyi Zainab Tenuche, Idoko Sunday Idoko, Ahmadu Saleh, Simon Azubuike Ubah

{"title":"A case of racemiform trichoblastoma in a dog.","authors":"Samson Eneojo Abalaka, Barde Angulu Thomas, Zakariya Audu, Sunday Augustine Ejeh, Nuhu Abdulazeez Sani, Oremeyi Zainab Tenuche, Idoko Sunday Idoko, Ahmadu Saleh, Simon Azubuike Ubah","doi":"10.1080/15321819.2022.2137809","DOIUrl":"https://doi.org/10.1080/15321819.2022.2137809","url":null,"abstract":"Trichoblastoma, which is common in dogs, is now occurring with other cellular changes outside the recognized forms to warrant their continuous evaluation for proper elucidation even as their causes largely remain unknown. A case at hand involved a 9-year-old Caucasian dog, which weighed 35 kg with chief complaint of a progressive bleeding mass on the scalp. The dog had an up-to-date vaccination record and all vital parameters were within optimum ranges. The surgical excision of the firm, solitary, and alopecic mass with traumatized upper surface revealed the presence of a well-demarcated and unencapsulated mass composed of grapes-like nests of basaloid epithelial cells within follicular stroma devoid of stromal necrosis, inflammatory cellular infiltration, and neoplastic epidermal connection. However, there was tissue necrosis, hemorrhages, and inflammatory cellular infiltrates on the exposed upper part of the traumatized growth. Immunohistochemical analysis showed positive reactivity to AE1/AE3, CK5/6, and p63 but negative immunoreactivity to CK7, CK20, CEA, and TTF-1. The histomorphological and immunohistochemical evaluation of the mass on the scalp of the dog suggested a solitary racemiform trichoblastoma with a traumatized exposed upper part despite basal cell carcinoma mimicry where histological diagnosis currently hold sway over immunohistochemical evaluation.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"44 1","pages":"90-102"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10469600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Significant alteration of IFN stimulated genes expression in MA104 cells infected with bovine rotavirus RF strain. 牛轮状病毒RF株感染MA104细胞后，IFN刺激基因表达显著改变。

Journal of immunoassay & immunochemistry Pub Date : 2023-01-02 DOI: 10.1080/15321819.2022.2118061

Ali Teimoori, Hessam Mirshahabi, Behzad Khansarinejad, Hoorieh Soleimanjahi, Hesam Karimi, Mojtaba Rasti, Somayeh Shatizadeh Malekshahi

{"title":"Significant alteration of IFN stimulated genes expression in MA104 cells infected with bovine rotavirus RF strain.","authors":"Ali Teimoori, Hessam Mirshahabi, Behzad Khansarinejad, Hoorieh Soleimanjahi, Hesam Karimi, Mojtaba Rasti, Somayeh Shatizadeh Malekshahi","doi":"10.1080/15321819.2022.2118061","DOIUrl":"https://doi.org/10.1080/15321819.2022.2118061","url":null,"abstract":"The pattern recognition receptors (PRRs) trigger signaling cascades, such as nuclear factor kappa B (NF-κB) and interferon regulatory factors (IRFs). Rotavirus (RV) countermeasures against innate responses and understanding of these processes will improve our knowledge regarding immunopathogenesis of RV infection. In this study, we investigated the effect of RV RF strain on the important ISG candidate genes engaging in virus infections for which little information is known in RV RF strain. To this end, MA104 cells were mock/infected with RF followed by incubation in the presence or absence of IFN-α and the expression of MX1, OAS1, STAT1, ISG15, and ISG56 mRNA was analyzed by real-time PCR. All of ISGs' mRNAs showed higher expression levels in IFN I treated cells compared to virus-infected cells except for ISG56. Infecting the cells with RV and treatment with IFN type I led to overexpression of ISG56 compared to cells were either infected with the virus or only treated with IFN I. In conclusion, we showed that the RV RF strain efficiently blocks type I IFN-induced gene expression particularly ISG15, MX1, STAT, and OSA1 as antiviral proteins. Furthermore, viruses may use some ISGs such as ISG 56 to regulate IFN I signaling pathway, negatively.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"44 1","pages":"56-65"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10455252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple and high -performance immobilization technique of membrane protein from crude cell lysate sample for a membrane-based immunoassay application. 一种简单、高效的膜蛋白固定技术，用于基于膜的免疫分析。

Journal of immunoassay & immunochemistry Pub Date : 2023-01-02 DOI: 10.1080/15321819.2022.2137420

Numfon Khemthongcharoen, Panapat Uawithya, Nutthapon Yookong, Mayuree Chanasakulniyom, Wutthinan Jeamsaksiri, Witsaroot Sripumkhai, Pattaraluck Pattamang, Ekachai Juntasaro, Ampol Kamnerdsook, Nongluck Houngkamhang, Chamras Promptmas

{"title":"A simple and high -performance immobilization technique of membrane protein from crude cell lysate sample for a membrane-based immunoassay application.","authors":"Numfon Khemthongcharoen, Panapat Uawithya, Nutthapon Yookong, Mayuree Chanasakulniyom, Wutthinan Jeamsaksiri, Witsaroot Sripumkhai, Pattaraluck Pattamang, Ekachai Juntasaro, Ampol Kamnerdsook, Nongluck Houngkamhang, Chamras Promptmas","doi":"10.1080/15321819.2022.2137420","DOIUrl":"https://doi.org/10.1080/15321819.2022.2137420","url":null,"abstract":"Membrane proteins are difficult to be extracted and to be coated on the substrate of the immunoassay reaction chamber because of their hydrophobicity. Traditional method to prepare membrane protein sample requires many steps of protein extraction and purification that may lead to protein structure deformation and protein dysfunction. This work proposes a simple technique to prepare and immobilize the membrane protein suspended in an unprocessed crude cell lysate sample. Membrane fractions in crude cell lysate were incorporated with the large unilamellar vesicle (LUV) that was mainly composed of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) before coating in the polystyrene plate by passive adsorption technique. Immunofluorescence staining and the Enzyme-Linked Immunosorbent Assay (ELISA) examination of a strictly conformation-dependent integral membrane protein, Myelin Oligodendrocyte Glycoprotein (MOG), demonstrate that LUV incorporated cell lysate sample obviously promotes MOG protein immobilization in the microplate well. With LUV incorporation, the dose-response curve of the MOG transfected cell lysate coating plate can be 2-9 times differentiated from that of the untransfected cell lysate coating plate. The LUV incorporated MOG transfected cell lysate can be efficiently coated in the microplate without carbonate/bicarbonate coating buffer assistance.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"44 1","pages":"76-89"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10469604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

To evaluate the feasibility of cadmium/tellurium (Cd/Te) quantum dots for developing N-terminal Natriuretic Peptide (NT-proBNP) in-vitro diagnostics. 评价镉碲量子点(Cd/Te)用于n端利钠肽(NT-proBNP)体外诊断的可行性。

Journal of immunoassay & immunochemistry Pub Date : 2023-01-02 DOI: 10.1080/15321819.2022.2103430

Vani M, Anugya Bhatt, Anoopkumar Thekkuveettil, Sanjay Ganapathy, Jeemon Panniyammakal, Harikrishnan Sivadasanpillai, Manoj Gopi

{"title":"To evaluate the feasibility of cadmium/tellurium (Cd/Te) quantum dots for developing N-terminal Natriuretic Peptide (NT-proBNP) in-vitro diagnostics.","authors":"Vani M, Anugya Bhatt, Anoopkumar Thekkuveettil, Sanjay Ganapathy, Jeemon Panniyammakal, Harikrishnan Sivadasanpillai, Manoj Gopi","doi":"10.1080/15321819.2022.2103430","DOIUrl":"https://doi.org/10.1080/15321819.2022.2103430","url":null,"abstract":"Quantum dots have been widely used for biomedical applications like imaging, targeted drug delivery, and in-vitro diagnostics for better sensitivity. In-vitro diagnostic, lateral flow-based assay systems are gaining attention in the field of biomarker analysis mainly due to ease of test and quick availability of results. In the study, the potential of water-soluble carboxylic (-COOH) functionalized photoluminescent Cadmium Telluride Quantum Dots (CdTe) nanoparticles for lateral flow-based detection of N-terminal Natriuretic Peptide (NT-proBNP) biomarker (for heart failure) detection has been evaluated. Monoclonal antibodies were conjugated with COOH functionalized CdTe with EDC-NHS coupling chemistry, and conjugation was confirmed using FTIR. The CdTe nanoparticle exhibited an emission maximum at 715 nm when it is excited with 375 nm. The COOH functionalized CdTe showed an antigen concentration-dependent linearity in the lateral flow applications when the dye was prepared freshly and used. However, a relative reduction in CdTe quantum dot fluorescence intensity with time was observed. Factors such as low stability could be due to the quenching of the fluorescence of CdTe. This limits its commercial viability as an in-vitro diagnostic tool; thus, modifications of the quantum dots are required to have a stable preparation for its commercial potential for quantifications.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"44 1","pages":"31-40"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10813111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Expression of B-cell activating factor in pediatric patients with immune thrombocytopenia: a single institutional series and review of literature. b细胞活化因子在儿童免疫性血小板减少症患者中的表达:单一机构系列和文献综述。

Journal of immunoassay & immunochemistry Pub Date : 2023-01-02 DOI: 10.1080/15321819.2022.2114363

Khaled A E Khalifa, Mahmoud A El-Hawy, Heba M Abo Zeid, Reem M El-Kholy

{"title":"Expression of B-cell activating factor in pediatric patients with immune thrombocytopenia: a single institutional series and review of literature.","authors":"Khaled A E Khalifa, Mahmoud A El-Hawy, Heba M Abo Zeid, Reem M El-Kholy","doi":"10.1080/15321819.2022.2114363","DOIUrl":"https://doi.org/10.1080/15321819.2022.2114363","url":null,"abstract":"B-cell-activating factor (BAFF) is a crucial cytokine supporting survival and differentiation of B cells. Dysregulation of BAFF is involved in the pathogenesis of B-cell related autoimmune diseases including immune thrombocytopenia (ITP). The aim of this study was to evaluate the significance of BAFF expression in pediatric ITP patients. Eighty pediatric patients with ITP are subdivided in three groups. Group I included (32 patients) diagnosed with acute ITP less than 3 months, group II (48 patients) diagnosed with persistent ITP (from 3 to 12 months) and chronic ITP (more than 12 months) and group III 20 healthy controls. Complete blood picture, autoimmune profile, antiplatelet antibodies, coagulation profile, bone marrow examination, and RT-PCR were performed to detect the expression for BAF for all participants in this study. BAFF expression levels significantly increased in cases rather than in controls. BAFF Expression Value significantly increased in groups I & II (3.10 ± 1.99&3.29 ± 2.58) compared to controls (0.83 ± 0.45) as p < .001 for both. On the other hand, groups I & II were comparable in BAFF Expression Value (p = .470). BAFF expression increased in ITP patients, implying a function in the disease's pathogenesis.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"44 1","pages":"41-55"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10511329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2