Journal of immunoassay & immunochemistry最新文献_第10页

Light chain 3 immunoexpression in psoriasis. 银屑病轻链3免疫表达。

Journal of immunoassay & immunochemistry Pub Date : 2022-07-04 Epub Date: 2022-01-07 DOI: 10.1080/15321819.2021.2018708

Rehab M Samaka, Alaa Marae, Manar Faried, Heba A S Bazid

{"title":"Light chain 3 immunoexpression in psoriasis.","authors":"Rehab M Samaka, Alaa Marae, Manar Faried, Heba A S Bazid","doi":"10.1080/15321819.2021.2018708","DOIUrl":"https://doi.org/10.1080/15321819.2021.2018708","url":null,"abstract":"Autophagy dysregulation is involved in many diseases. The implication of autophagy in psoriasis pathogenesis is still uncertain. To investigate the role of Light Chain 3 (LC3), a good marker for autophagy, in psoriatic skin based on immunohistochemical study and correlate its expression - for the first time to the best of our knowledge - to clinicopathological data Prospective case-control study was conducted on 60 subjects (30 control, 30 psoriasis patients). Skin biopsies from control, lesional, and perilesional skin were processed for routine histopathological examination and LC3 immunoreaction assessment. There was a significant upregulation of the epidermal and dermal LC3 immunoreaction in the lesional skin compared with the control and perilesional skin specimens (P < .001). A significant positive correlation between the epidermal and dermal LC3 H scores in the lesional and perilesional skin was recorded. There was a non-significant relationship between the H score in the lesional skin and disease severity. LC3 could be considered in psoriasis pathogenesis; however, LC3 was not related to the severity of the disease. The findings might offer a novel target therapy for psoriasis patients.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"43 4","pages":"365-383"},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39655537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of cold-inducible RNA binding protein in psoriasis. 银屑病冷诱导RNA结合蛋白的表达。

Journal of immunoassay & immunochemistry Pub Date : 2022-07-04 Epub Date: 2022-02-22 DOI: 10.1080/15321819.2022.2039183

Heba Bazid, Mohamed Shoeib, Asmaa Elsayed, Mohammed Mostafa, May Shoeib, Eman M Abd El Gayed, Rania Abdallah

{"title":"Expression of cold-inducible RNA binding protein in psoriasis.","authors":"Heba Bazid, Mohamed Shoeib, Asmaa Elsayed, Mohammed Mostafa, May Shoeib, Eman M Abd El Gayed, Rania Abdallah","doi":"10.1080/15321819.2022.2039183","DOIUrl":"https://doi.org/10.1080/15321819.2022.2039183","url":null,"abstract":"Psoriasis is an immune-mediated skin disease with a potential morbidity in patients. Cold-inducible RNA binding protein (CIRP) is a stress responsive protein having diverse roles in cancer and inflammation. This study aimed to evaluate the expression of CIRP, (serum and tissue), in psoriasis patients and to correlate this expression to the clinico-pathological data of the patients. The serum level and tissue expression of CIRP were compared between 20 patients and 20 healthy controls. Additionally, the association between CIRP level and various clinicopathological parameters was done. The serum level of CIRP was measured by enzyme-linked immunosorbent assay (ELISA) while its tissue expression was detected via immunohistochemistry. CIRP was expressed in the epidermis of all studied cases and controls with nuclear localization. A significant difference in its epidermal expression between lesional, perilesional cases and controls was observed. It was higher in control epidermis than perilesional skin and the lowest in lesional skin. Conversely, the serum CIRP level was significantly higher in psoriasis patients compared to healthy subjects. CIRP seemed to have a significant pathologic role in psoriasis patients with evident difference in its intracellular and extracellular expression levels suggesting a potential difference it its function.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"43 4","pages":"384-402"},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39941929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Immunolocalization of CD1a expressing dendritic cells in sinonasal polyposis. 表达CD1a树突状细胞在鼻窦息肉病中的免疫定位。

Journal of immunoassay & immunochemistry Pub Date : 2022-07-04 Epub Date: 2022-02-11 DOI: 10.1080/15321819.2022.2034645

Rachel Jayasekhar, John Kandam Kulathu Mathew, Zorem Sangi, Sam David Marconi, Vedantam Rupa, Suganthy Rabi

{"title":"Immunolocalization of CD1a expressing dendritic cells in sinonasal polyposis.","authors":"Rachel Jayasekhar, John Kandam Kulathu Mathew, Zorem Sangi, Sam David Marconi, Vedantam Rupa, Suganthy Rabi","doi":"10.1080/15321819.2022.2034645","DOIUrl":"https://doi.org/10.1080/15321819.2022.2034645","url":null,"abstract":"Sinonasal polyps are benign projections of edematous nasal mucosa lined by respiratory epithelium. Langerhans cells (LCs) belonging to the dendritic cell family located in respiratory epithelium are involved in antigen presentation and maintenance of local immunological homeostasis. This study aims to elucidate the morphology and distribution of CD1a positive LCs in normal nasal mucosa and compare the same with polypoid nasal mucosa by immunohistochemistry. Normal nasal mucosa (n = 20) was obtained from patients who underwent septoplasty for deviated nasal septum. Polypoid nasal mucosa (n = 22) was obtained from patients with chronic rhinosinusitis (CRS) or allergic fungal rhinosinusitis who underwent excision of nasal polyps. The tissues obtained were processed for immunohistochemistry and stained with CD1a-EP80 Rabbit monoclonal antibody. In the tissues studied, CD1a positive LCs were observed in both the epithelium and lamina propria. Different morphological subtypes of LCs were noted in the epithelium. The cells were distributed adjacent to walls of subepithelial capillaries and cysts. The median number of CD1a positive LCs was significantly higher in polypoid category (13.5 per mm2) as compared with normal nasal mucosa (2.5per mm2) (p = .001). Presence of CD1a positive LCs in polypoid nasal mucosa hints at a critical immunological role in the etiopathogenesis of nasal polyps.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"43 4","pages":"403-419"},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39605818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of SET oncoprotein in hepatocellular carcinoma: An immunohistochemical study. SET癌蛋白在肝细胞癌中的作用:免疫组织化学研究。

Journal of immunoassay & immunochemistry Pub Date : 2022-07-04 Epub Date: 2022-02-13 DOI: 10.1080/15321819.2022.2034646

Marwa Gadallah, Nancy Yousef Asaad, Mohammed Shabaan, Shimaa Saad Elkholy, Manar Yousef Samara, Doha Taie

{"title":"Role of SET oncoprotein in hepatocellular carcinoma: An immunohistochemical study.","authors":"Marwa Gadallah, Nancy Yousef Asaad, Mohammed Shabaan, Shimaa Saad Elkholy, Manar Yousef Samara, Doha Taie","doi":"10.1080/15321819.2022.2034646","DOIUrl":"https://doi.org/10.1080/15321819.2022.2034646","url":null,"abstract":"Hepatocellular carcinoma (HCC) is the most prevalent primary cancer of the liver and it is the fourth most common cause of cancer related death worldwide. In Egypt, liver cancer constitutes the most common cause of mortality-related cancer. This study aimed to evaluate the immunohistochemical expression of SET oncoprotein in HCC tissues in comparison with its expression in non tumorous liver tissues and to correlate its expression with clinicopathological parameters. This study investigated 100 cases of HCC (including tumorous and non tumorous tissues). One hundred percent of tumorous and non-tumorous tissues were positive for SET expression. The mean and median values of H-score for SET expression were higher in tumorous than non tumorous tissues (P = .03). Higher SET expression was significantly correlated with larger tumor size (P = .012), positive lymphovascular invasion (P = .028), and shorter overall survival (P < .001). SET expression in tumor tissues is the most independent factor to affect the overall survival of HCC patients. SET plays a role in hepatocarcinogenesis proved by the increase of SET expression from non-tumorous to tumorous tissues. Also, SET can be used as a prognostic indicator and a novel target therapy in HCC patients.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"43 4","pages":"420-434"},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39916430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sero-evidence of silent Japanese Encephalitis Virus infection among inhabitants of Ilorin, North-central Nigeria: a call for active surveillance. 尼日利亚中北部伊洛林居民中无症状日本脑炎病毒感染的血清证据:呼吁进行积极监测。

Journal of immunoassay & immunochemistry Pub Date : 2022-05-04 Epub Date: 2021-11-23 DOI: 10.1080/15321819.2021.1993897

Augustine Okechukwu Udeze, Mutiat B Odebisi-Omokanye

{"title":"Sero-evidence of silent Japanese Encephalitis Virus infection among inhabitants of Ilorin, North-central Nigeria: a call for active surveillance.","authors":"Augustine Okechukwu Udeze, Mutiat B Odebisi-Omokanye","doi":"10.1080/15321819.2021.1993897","DOIUrl":"https://doi.org/10.1080/15321819.2021.1993897","url":null,"abstract":"Infection by Japanese Encephalitis Virus (JEV) causes viral encephalitis in humans. It is endemic in Asia and western Pacific regions with no reported case in Nigeria so far. This study aimed at detecting evidence of circulation and pattern of exposure of individuals in Ilorin, Nigeria to JEV. A total of 139 febrile patients attending outpatient departments of Sobi specialist hospital, Civil service hospital and University of Ilorin health center participated in the study. About 5 ml of venous blood was collected from each participant and the plasma tested for the presence of anti-JEV IgG antibody using Enzyme Linked Immunosorbent Assay (ELISA) technique. Anti-JEV IgG antibody was detected in 32% (45/139) of the participants. Males were more exposed (37%) than female participants (27.3%). Similarly, participants with travel history outside of the country were more exposed (44.4%) than those without travel history (29.5%). Further analysis on the basis of age, marital and working status showed no statistical association between these variables and anti-JEV positivity (p˃0.05). This study has provided serological evidence of the circulation of JEV among Ilorin inhabitants and therefore calls for active surveillance for the infection in order to forestall any future major outbreak.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"43 3","pages":"250-258"},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39759902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic significance of serum telomerase activity in the monitoring of hepatitis B viral infection. 血清端粒酶活性在乙型肝炎病毒感染监测中的预后意义。

Journal of immunoassay & immunochemistry Pub Date : 2022-05-04 Epub Date: 2021-12-03 DOI: 10.1080/15321819.2021.2004162

A A Adelakun, I O Adediji, O J Idowu, T F Jegede, A S Oluremi, P O Adepoju, O A Olaniyan

{"title":"Prognostic significance of serum telomerase activity in the monitoring of hepatitis B viral infection.","authors":"A A Adelakun, I O Adediji, O J Idowu, T F Jegede, A S Oluremi, P O Adepoju, O A Olaniyan","doi":"10.1080/15321819.2021.2004162","DOIUrl":"https://doi.org/10.1080/15321819.2021.2004162","url":null,"abstract":"Hepatitis B viral infection could be complicated by hepatocellular degeneration, liver cirrhosis, and cancer. A total of 87 participants - 29 each of symptomatic and asymptomatic hepatitis B positive, and hepatitis B negative individuals (controls) - were recruited, and their serum samples were evaluated for serum telomerase (a biomarker for cell aging and tumorigenesis), alpha fetoprotein, and liver enzymes. Serum telomerase of the symptomatic group was higher than that of the asymptomatic group and the control (P < .001). Serum α-fetoprotein in the symptomatic group was also higher than the asymptomatic group and the controls (P < .001). The mean AST value for the symptomatic test group was higher than the asymptomatic test group and the control (P < .001). The mean ALT value for the symptomatic test group was higher than the asymptomatic test group and the control (P < .001). However, serum α-fetoprotein, AST, and ALT in the asymptomatic group were not significantly different from the controls. Serum telomerase activity was higher in symptomatic and asymptomatic HBV subjects compared with controls; this provides better information than AFP and liver enzymes that were only higher in symptomatic subjects. Serum telomerase activity could therefore be used as a marker in predicting the onset of hepatocarcinogenesis.Abbreviation listHBV: Hepatitis B virus; AFP: Alpha fetoprotein; ALT: Alanine transaminase; AST: Aspartate transaminase; HCC: Hepatocellular carcinoma; ELISA: Enzyme-linked immunosorbent assay; CLD: Chronic liver disease; CMV: Cytomegalovirus; TERT: Telomerase reverse transcriptase; TERC: Telomerase RNA component; WHO: World Health Organization; BUHREC: Babcock University Health Research Ethics Committee; CTL: Cytotoxic T-lymphocyte.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"43 3","pages":"299-307"},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39802663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of complete blood cell count parameters and lymphocyte-related ratios in patients with Opioid Use Disorder. 阿片类药物使用障碍患者全血细胞计数参数和淋巴细胞相关比值的评价。

Journal of immunoassay & immunochemistry Pub Date : 2022-05-04 Epub Date: 2021-11-29 DOI: 10.1080/15321819.2021.2001001

Sema Baykara, Şule Şirin Berk, Şüheda Kaya, Davut Ocak

{"title":"Evaluation of complete blood cell count parameters and lymphocyte-related ratios in patients with Opioid Use Disorder.","authors":"Sema Baykara, Şule Şirin Berk, Şüheda Kaya, Davut Ocak","doi":"10.1080/15321819.2021.2001001","DOIUrl":"https://doi.org/10.1080/15321819.2021.2001001","url":null,"abstract":"Complete blood count (CBC) parameters and lymphocyte-related ratios are found to be associated with inflammation and increased cytokine production. In Opioid Use Disorder (OUD), CBC parameters can be examined as the inflammatory (oxidative stress) biomarkers which could be used as an objective marker to support the OUD diagnosis and could be used as a disease severity marker. CBC parameters of 142 patients with OUD were examined. Lymphocytes, monocytes, basophiles, eosinophils and platelet counts (PLT), red blood cell levels (HGB), mean erythrocyte volume (MCV) and lymphocyte-related ratios were calculated. The control group consisted of 140 healthy individuals. WBC, neutrophil, lymphocyte, monocyte, platelet count, neutrophil, basophil percentage, neutrophil/ lymphocyte ratio (NLR), monocyte/lymphocyte ratio (MLR) values were significantly higher in OUD group. Lymphocyte, basophil percentage, basophile/lymphocyte ratio (BLR), red blood cell (RBC), hemoglobin were significantly higher in control group. Mean platelet volume (MPV), eosinophil/lymphocyte ratio (ELR), platelet/ lymphocyte ratio (PLR) values were not significant yet higher in the OUD group. CBC and related parameters (WBC, neutrophil, lymphocyte, monocyte, platelet count, neutrophil, basophil percentage, NLR, MLR) were found to be significantly higher in the OUD group. Evaluation of CBC parameters could be useful in treatment and follow-up of patients with OUD.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"43 3","pages":"259-270"},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39673487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Hypothyroidism affect progression and worse outcomes of breast cancer but not ovarian cancer. 甲状腺功能减退会影响乳腺癌的进展和更糟糕的结果，但不会影响卵巢癌。

Journal of immunoassay & immunochemistry Pub Date : 2022-05-04 Epub Date: 2021-11-17 DOI: 10.1080/15321819.2021.2001003

Mohamed M Elgebaly, Asmaa R Abdel-Hamed, Noha M Mesbah, Dina M Abo-Elmatty, Amr Abouzid, Mohamed A Abdelrazek

{"title":"Hypothyroidism affect progression and worse outcomes of breast cancer but not ovarian cancer.","authors":"Mohamed M Elgebaly, Asmaa R Abdel-Hamed, Noha M Mesbah, Dina M Abo-Elmatty, Amr Abouzid, Mohamed A Abdelrazek","doi":"10.1080/15321819.2021.2001003","DOIUrl":"https://doi.org/10.1080/15321819.2021.2001003","url":null,"abstract":"Some studies suggest that thyroid hormones and disorders can influence breast (BC) and ovarian (OC) cancers risks. However, studies regarding their effect on these tumors progression are limited. Thyroid-stimulating hormone (TSH), T4, free T4 (FT4), T3, and free T3 (FT3) were detected in patients with BC, OC, benign breast and ovary diseases, and healthy controls using highly sensitive chemiluminescence assay. In contrast to OC, hypothyroidism prevalence was associated with BC late stage (11/24 vs. 2/46), high grade (11/23 vs. 4/47), lymph node invasion (11/42 vs. 0/28), positive distant metastasis (11/25 vs. 1/45), and large tumor size (14/25 vs. 1/45) compared to tumor early stages, low grades, negative lymph node, and distant metastasis and small size, respectively. Patients with late stage, high grade, large tumor size, positive lymph nodes, or positive distant metastasis were significantly (P < 0.05) associated with elevated levels of TSH and decreased levels of T4, FT4, T3, and FT3. There were both significant positive correlation of serum TSH and significant inverse correlation of T4, FT4, T3, and FT3 with these tumor worse outcomes. In conclusion, our results identify hypothyroidism as potentially important prognostic factor in BC not in OC that is associated with poor outcomes of BC patients.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"43 3","pages":"288-298"},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39740526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Performance evaluation of the new Chemiluminescence Immunoassay CL-1200i Thyroid Panel. 新型化学发光免疫分析法CL-1200i甲状腺检测组的性能评价。

Journal of immunoassay & immunochemistry Pub Date : 2022-05-04 Epub Date: 2021-12-18 DOI: 10.1080/15321819.2021.2017301

Eleonora Nicolai, Marzia Nuccetelli, Serena Sarubbi, Valerio Basile, Marco Alfonso Perrone, Alessandro Terrinoni, Marilena Minieri, Massimo Pieri, Sergio Bernardini

{"title":"Performance evaluation of the new Chemiluminescence Immunoassay CL-1200i Thyroid Panel.","authors":"Eleonora Nicolai, Marzia Nuccetelli, Serena Sarubbi, Valerio Basile, Marco Alfonso Perrone, Alessandro Terrinoni, Marilena Minieri, Massimo Pieri, Sergio Bernardini","doi":"10.1080/15321819.2021.2017301","DOIUrl":"https://doi.org/10.1080/15321819.2021.2017301","url":null,"abstract":"Aim of this work was to verify the analytical performance of thyroid panel tests measured by chemiluminescence immunoassay (CLIA) CL-1200i and to validate its efficacy as laboratory test for thyroid disorder.Serum samples were obtained by standard centrifugation, thawed and assayed in a blinded fashion, and in a single batch. This study compares the values of thyroid panel tests measured by Mindray CL-1200i chemiluminescent system to the Abbott platforms for TSH, FT3, FT4, and Beckman Coulter for Tg, TgAb, and TPOAb on patient serum samples. A total of 180 randomly selected patients including both hospitalized and ambulatory patients from the Policlinico Tor Vergata (PTV) of the University of Rome Tor Vergata were used. In all analyses performed, the thyroid panel tests of the Mindray platform showed discriminative ability to quantitatively assess the analyte involved in thyroid disease and disorder. This study verified that Mindray CL-1200i chemiluminescent system thyroid panel tests is a valid method for obtaining a quantitative analysis of thyroid disorders. It showed high diagnostic efficiency and could represent a valid tool with a potential reduction in time and workload for the diagnosis.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"43 3","pages":"333-345"},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39740745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of pulmonary tuberculosis on natural anticoagulant activity in therapy-naïve Ghanaian adults; a case-control study. 肺结核对therapy-naïve加纳成人天然抗凝血活性的影响病例对照研究。

Journal of immunoassay & immunochemistry Pub Date : 2022-05-04 Epub Date: 2022-01-26 DOI: 10.1080/15321819.2021.2001002

Felix Osei-Boakye, Otchere Addai-Mensah, Michael Owusu, Abdul-Razak Saasi, Samuel Kwasi Appiah, Charles Nkansah, Yaw Amo Wiafe, Alexander Yaw Debrah

{"title":"Effect of pulmonary tuberculosis on natural anticoagulant activity in therapy-naïve Ghanaian adults; a case-control study.","authors":"Felix Osei-Boakye, Otchere Addai-Mensah, Michael Owusu, Abdul-Razak Saasi, Samuel Kwasi Appiah, Charles Nkansah, Yaw Amo Wiafe, Alexander Yaw Debrah","doi":"10.1080/15321819.2021.2001002","DOIUrl":"https://doi.org/10.1080/15321819.2021.2001002","url":null,"abstract":"Tuberculosis constitutes a global emergency as it affects one-third of the world's inhabitants. Although Pulmonary tuberculosis (PTB) is curable, immunological responses to the infection induce several hematological derangements. This study evaluated the effect of PTB on natural anticoagulant activity and CBC indices. Ninety adults were recruited: 60 PTB patients and 30 non-TB controls. Blood specimens from each participant was tested for Proteins C and S, Antithrombin-III and CBC. Pulmonary TB was associated with significantly reduced Protein C activity (101.46 [87.61-128.3] vs 121.44 [99.50-149.8] IU/L, p= 0.038), RBC (p< 0.0001), HgB (p= 0.0019), HCT (p< 0.0001), MCV (p= 0.0133) and PDW (p< 0.0001) compared to controls. Conversely, PTB patients were associated with significantly increased MCH (p= 0.0086), TWBC (p= 0.0047), Abs. GRAN (p= 0.0226), RDW-CV (p< 0.0001), MCHC (p< 0.0001) and MPV (p= 0.0027) compared to controls. The PTB patients were disproportionately affected with anemia (91.7%, p= 0.001), erythrocytopenia (75.0%, p≤ 0.001) and reduced HCT (80.0%, p≤ 0.001). The frequency of thrombocytosis, leucocytosis, and granulocytosis (50.0%, p= 0.013; 23.3%, p= 0.013; 18.3%, p= 0.025; respectively) in PTB patients were significantly higher than in controls. PTB predisposes to hypercoagulability and causes derangements in erythrocytes, leucocytes, and thrombocytes, and disproportionately causes anemia. Measurement of Protein C activity and CBC indices are useful in the management of PTB patients.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"43 3","pages":"271-287"},"PeriodicalIF":0.0,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39723369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2