Journal of immunoassay & immunochemistry最新文献

Oxidative-antioxidative balance and stromal cell-derived factor-4 in generalized anxiety disorder: implications for pathophysiology and biomarker potential. 广泛性焦虑障碍的氧化-抗氧化平衡和基质细胞衍生因子-4：病理生理学和生物标志物潜能的含义。

Journal of immunoassay & immunochemistry Pub Date : 2025-10-06 DOI: 10.1080/15321819.2025.2567856

Sidika Baziki Çetİn, Ülker Atilan Fedai, Öznur Akil, İsmail Koyuncu, Merve Doğan Koçbaba

{"title":"Oxidative-antioxidative balance and stromal cell-derived factor-4 in generalized anxiety disorder: implications for pathophysiology and biomarker potential.","authors":"Sidika Baziki Çetİn, Ülker Atilan Fedai, Öznur Akil, İsmail Koyuncu, Merve Doğan Koçbaba","doi":"10.1080/15321819.2025.2567856","DOIUrl":"https://doi.org/10.1080/15321819.2025.2567856","url":null,"abstract":"This study explores the relationship between oxidative - antioxidative balance and stromal cell-derived factor-4 (SDF-4) levels in individuals with Generalized Anxiety Disorder (GAD), aiming to clarify their roles in the disorder's pathophysiology. By examining these biomarkers, the research investigates whether oxidative stress mechanisms contribute to GAD. A cross-sectional study was conducted with 43 GAD patients and 40 age- and sex-matched healthy controls. Participants were assessed using the Beck Anxiety Inventory (BAI) and the Structured Clinical Interview for DSM-5. Serum levels of SDF-4, total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were measured. GAD patients showed significantly higher levels of SDF-4, TAS, TOS, and OSI compared to controls (p < 0.001). No significant differences were found in other hematological parameters. In the GAD group, SDF-4 levels were not significantly correlated with anxiety severity or oxidative markers. ROC analysis indicated good diagnostic performance of SDF-4 for GAD (AUC = 0.870). The findings suggest that oxidative - antioxidative imbalance and elevated SDF-4 levels may play a role in GAD. SDF-4 might represent a compensatory response to oxidative stress. Elevated TAS levels may indicate enhanced antioxidant defense. Further studies are needed to clarify the mechanistic and therapeutic implications.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative immunodiagnostic performance of AgB1-derived synthetic peptides in human cystic echinococcosis. agb1合成肽对人囊性包虫病的比较免疫诊断性能。

Journal of immunoassay & immunochemistry Pub Date : 2025-09-05 DOI: 10.1080/15321819.2025.2556426

Javier Magnone, Maite Folle, Sebastián Miles, Sofía Lagos, María Clara González-Porcile, Ana Hernández, Daniel Da-Rosa, Ana María Ferreira, Cecilia Sóñora, Gustavo Mourglia-Ettlin

{"title":"Comparative immunodiagnostic performance of AgB1-derived synthetic peptides in human cystic echinococcosis.","authors":"Javier Magnone, Maite Folle, Sebastián Miles, Sofía Lagos, María Clara González-Porcile, Ana Hernández, Daniel Da-Rosa, Ana María Ferreira, Cecilia Sóñora, Gustavo Mourglia-Ettlin","doi":"10.1080/15321819.2025.2556426","DOIUrl":"https://doi.org/10.1080/15321819.2025.2556426","url":null,"abstract":"Immunoassays are complementary diagnostic tools in human cystic echinococcosis (CE) despite sensitivity/specificity limitations, and synthetic peptides have been suggested to potentially overcome disadvantages reported for traditional antigens. Herein, a systematic study comparing the immunodiagnostic performance of AgB1 versus synthetic peptides derived from its sequence was carried out. Thus, a eukaryotic-expressed recombinant AgB1 was assessed, together with a reported synthetic peptide (p176, N-terminal portion of AgB1) and two new peptides within p176 (namely pB1a and pB1b) corresponding to predicted linear B-cell epitopes. Immunodiagnostic performances were evaluated by ELISA using a large collection of human sera from CE patients, healthy donors, and individuals with other parasitoses; assessing the sensitivity, specificity, indeterminacy, cross-reactivity, and diagnostic efficiency of the assay according to each antigen. Results suggest that peptides retaining most of the original protein sequence and 3D-structure display better overall diagnostic efficiencies (IgGt values for rAgB1, p176, pB1a, and pB1b were 66.1%, 60.4%, 54.7%, and 49.0%, respectively), with a small N-terminal portion of AgB1 being immunodominant. Additionally, detection of specific IgG4 antibodies significantly reduced cross-reactivity, while improving sensitivity (IgGt-IgG4 values for rAgB1, p176, and pB1a were 37.5%-42.7%, 24.0%-29.2%, and 11.5%-24.0%, respectively). Valuable information was obtained for rationally design novel peptide-based assays for CE immunodiagnosis.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inflammatory and miRNA-based signatures in Hashimoto's thyroiditis and non-immune hypothyroidism. 桥本甲状腺炎和非免疫性甲状腺功能减退的炎症和mirna特征。

Journal of immunoassay & immunochemistry Pub Date : 2025-09-01 DOI: 10.1080/15321819.2025.2553574

Ali Abed Lafta, Farhan Abood Risan, Suhad Hassan Aubaid

{"title":"Inflammatory and miRNA-based signatures in Hashimoto's thyroiditis and non-immune hypothyroidism.","authors":"Ali Abed Lafta, Farhan Abood Risan, Suhad Hassan Aubaid","doi":"10.1080/15321819.2025.2553574","DOIUrl":"https://doi.org/10.1080/15321819.2025.2553574","url":null,"abstract":"Hypothyroidism encompasses both autoimmune forms, notably Hashimoto's thyroiditis (HT), and nonimmune hypothyroidism (NIHT), each driven by distinct molecular mechanisms. Despite overlapping clinical features, their specific molecular differences remain underexplored. This study aimed to compare the expression of inflammatory biomarkers (Calprotectin, Cyclophilin A, Endocan, Procalcitonin) and microRNAs (miR-223, miR-711) among HT, NIHT, and healthy individuals, evaluating their diagnostic relevance. A total of 120 participants were enrolled: 40 with HT, 40 with NIHT, and 40 healthy controls. Serum inflammatory markers were quantified via ELISA, and miRNA levels assessed using qRT-PCR normalized to U6 RNA. Statistical analyses included ANOVA, correlation, and ROC curve evaluation. Significant group differences were found in inflammatory marker levels (p < 0.001), with NIHT patients showing higher concentrations than HT and controls. miR-223 was notably upregulated in both HT and NIHT groups (p = 0.006), whereas miR-711 showed a non-significant downregulation. ROC analysis revealed miR-223 had the highest diagnostic value in distinguishing HT (AUC = 0.84) and NIHT (AUC = 0.85) from controls, outperforming other markers. Cyclophilin A also demonstrated strong discriminatory capability. These findings suggest that combined profiling of inflammatory markers and miRNAs - especially miR-223 and Cyclophilin A holds promise for improved diagnosis and understanding of hypothyroid subtypes.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-18"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144957031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of antimicrobial peptide Human β Defensin-2 with clue cell appearance and the type of culture isolate in women with spontaneous and recurrent pregnancy losses. 抗微生物肽人β防御素-2与自发性和复发性流产妇女线索细胞形态和培养分离物类型的相关性

Journal of immunoassay & immunochemistry Pub Date : 2025-08-21 DOI: 10.1080/15321819.2025.2549270

Hind M Al-Khazrajy, Siham Sh Al-Salihi

{"title":"Correlation of antimicrobial peptide Human β Defensin-2 with clue cell appearance and the type of culture isolate in women with spontaneous and recurrent pregnancy losses.","authors":"Hind M Al-Khazrajy, Siham Sh Al-Salihi","doi":"10.1080/15321819.2025.2549270","DOIUrl":"https://doi.org/10.1080/15321819.2025.2549270","url":null,"abstract":"Human β Defensin-2 (HBD2) is an Antimicrobial peptide (AMP) that serves a dual function in host defense and fertility. On the other hand, a plethora of microbial infectious agents have been associated with an increased risk of spontaneous abortion (SA). This study examined the impact of HBD-2 on women with SA with a particular focus on its relationship with clue cells and other microbial infections. 100 women aged 17-50 with a history of SA participated. We utilized two vaginal swabs. They were cultivated first. The Vitek2 technique was used after biochemical tests identified the isolates. vaginosis diagnosed by Amsel criteria. Serum HBD-2 levels were measured using Enzyme-linked immunosorbent assay (ELISA). An association was observed between bacterial vaginosis and SA in the second trimester. HBD-2 levels fell with age. Women with five or more abortions had lower HBD-2 levels (p = 0.034). In cases containing clue cells, HBD-2 averaged 586.0 pg/mL, whereas in cases without clue cells, it decreased (p = 0.007). Gram-negative bacterial infections decreased HBD-2. During fungal infection, HBD-2 levels dropped to 176.1 pg/ml. A significant relationship was observed between HBD2 levels and vaginitis, suggesting its potential as a biomarker and diagnostic tool for reproductive health.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Burcu Kök Kendirlioğlu, MD, PhD -Early Career Prize Winner 2024. Burcu Kök Kendirlioğlu，医学博士，博士- 2024年早期职业奖得主。

Journal of immunoassay & immunochemistry Pub Date : 2025-04-20 DOI: 10.1080/15321819.2025.2496044

引用次数: 0

Evaluation of Ki67 in pathological prognostic staging of breast cancer: a tertiary care center study. Ki67在乳腺癌病理预后分期中的评价：一项三级保健中心研究。

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2025-01-15 DOI: 10.1080/15321819.2025.2451211

Rashmi Wankhade, Arvind Bhake, Nandkishor Bankar, Yugeshwari Tiwade

{"title":"Evaluation of Ki67 in pathological prognostic staging of breast cancer: a tertiary care center study.","authors":"Rashmi Wankhade, Arvind Bhake, Nandkishor Bankar, Yugeshwari Tiwade","doi":"10.1080/15321819.2025.2451211","DOIUrl":"10.1080/15321819.2025.2451211","url":null,"abstract":"Background: The rising global burden of breast cancer demands early detection and effective treatment, with a focus on prognostic and predictive markers. The eighth edition of the American Joint Committee on Cancer staging manual introduced a new prognostic staging system to increase the predictive power of the existing anatomical staging system of breast cancer. The current study aimed to establish the correlation between Ki67 expression with molecular subtypes and with the pathological prognostic stage of invasive ductal carcinoma.Materials and methods: A total of 40 patients were included in the study with samples from 32 modified radical mastectomies and 8 biopsies. Hematoxylin and Eosin staining, histopathological analysis and Ki67 immunostaining were conducted. Descriptive and inferential statistical analyses were performed.Results: Bloom Richardson Grade II was the predominant histological grade. In Grade II cases, 15 of 24 had a Ki67 labeling index of 26-45%, while 6 exceeded 45% (p = 0.001). Pathological prognostic staging reclassified 27 cases, with 24 (75%) downstaged, 3 (9.38%) upstaged, and 5 (15.63%) retaining their clinical stage.Conclusions: Ki67 immunohistochemistry is an effective tool for assessing proliferative activity of invasive ductal carcinoma, aiding in pathological prognostic stage stratification and offering insights into tumor biology.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"186-200"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparison between different chemical fractionation methods for immunoglobulin preparation. 制备免疫球蛋白不同化学分馏方法的比较。

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2025-01-11 DOI: 10.1080/15321819.2025.2450664

Abbasali Salarifar, Pooria Safarzadeh Kozani, Mohammad Javad Rasaee

{"title":"A comparison between different chemical fractionation methods for immunoglobulin preparation.","authors":"Abbasali Salarifar, Pooria Safarzadeh Kozani, Mohammad Javad Rasaee","doi":"10.1080/15321819.2025.2450664","DOIUrl":"10.1080/15321819.2025.2450664","url":null,"abstract":"Background: Application of antibodies in therapeutics and diagnostics are growing Continually. Herein, we aimed to find the most qualified immunoglobulin (Ig) chemical preparation method.Methods: A rabbit was immunized against recombinant SARS-CoV-2 nucleocapsid (NP) and reactive polyclonal antibodies were prepared using the ammonium sulfate (AS), caprylic acid (CA), polyethylene glycol (PEG), and caprylic acid/ammonium sulfate (CA/AS) methods. Different antibody solutions were analyzed by SDS-PAGE and subsequently quantified by ImageJ software for further analysis in terms of Ig purity, Ig recovery, and albumin impurity. Ultimately, the prepared antibodies were assessed via Western blotting and ELISA to evaluate their ability to bind NP.Results: Prepared Ig solutions via the CA/AS method had the highest Ig purity (followed by CA, PEG, and AS) and lowest albumin impurity (followed by CA, AS, and PEG). The PEG method had the highest recovery followed by AS, CA, and CA/AS methods. Moreover, antibodies prepared via different methods demonstrated comparable binding capacities to NP in ELISA and Western blotting.Conclusions: CA/AS, closely followed by CA, proved to be the most qualified method for the preparation of Ig yielding the highest Ig purity while the PEG method resulted in the highest Ig recovery rate.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"169-185"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimate of circRNAs and microRNAs synergies on clinical advance of psoriasis. circRNAs和microRNAs在银屑病临床进展中的协同作用评估。

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2024-12-30 DOI: 10.1080/15321819.2024.2447726

Amany A Saleh, Safaa I Tayel, Wafaa A Shehata, Eman A El-Masry, Huda I Abd-Elhafiz, Mohammed G Elhelbawy, Sherin S El-Naidany

{"title":"Estimate of circRNAs and microRNAs synergies on clinical advance of psoriasis.","authors":"Amany A Saleh, Safaa I Tayel, Wafaa A Shehata, Eman A El-Masry, Huda I Abd-Elhafiz, Mohammed G Elhelbawy, Sherin S El-Naidany","doi":"10.1080/15321819.2024.2447726","DOIUrl":"10.1080/15321819.2024.2447726","url":null,"abstract":"Background: Noncoding RNAs have recently proven their contributing role in the pathogenesis of numerous diseases. The etiology of psoriasis is still not well defined, and genetic and environmental factors are blamed. We meant to explore the association of circRNAs/microRNAs in psoriasis and inspect their correlation with the clinical features of the disease.Design and methods: This study included 166 subjects: 83 cases with plaque psoriasis and 83 age- and sex-matched healthy persons as the control group. The expression levels of circRNA000102, circRNA0045272, circRNA0084764, and microRNAs -93,181, 16a, and 21 were estimated by quantitative real-time PCR.Results: CircRNA0045272 was ten-fold up-regulated, whereas circRNA0084764 and circRNA000102 were about two-fold downregulated in patients vs. control. MicroRNAs -93 and 181 were significantly downregulated in patients vs. control, whereas microRNA -16a and 21 were up-regulated in patients vs. control. CircRNA0045272 was positively correlated with microRNA 181, and circRNA0084764 was positively correlated with microRNA 93 and microRNA 181. CircRNA0045272 had the highest sensitivity (98.8%) and specificity (93.98%), and microRNA-16a had sensitivity (98.80%) with specificity (93.98%).Conclusion: We assume that circRNAs can predict psoriasis occurrence and progression by sponging a set of microRNAs and can modulate several genes that disturb immune cell balance and inflammatory elements in psoriasis.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"46 2","pages":"147-168"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral human papillomavirus infection and genotyping in a cohort of people living with HIV. 一组HIV感染者的口腔人乳头瘤病毒感染和基因分型

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2024-12-23 DOI: 10.1080/15321819.2024.2441778

O F Adebayo, F J Owotade, O A Folarin, O A Oninla, E O Oyetola

{"title":"Oral human papillomavirus infection and genotyping in a cohort of people living with HIV.","authors":"O F Adebayo, F J Owotade, O A Folarin, O A Oninla, E O Oyetola","doi":"10.1080/15321819.2024.2441778","DOIUrl":"10.1080/15321819.2024.2441778","url":null,"abstract":"This study is aimed at determining the prevalence of oral HPV infection and the risk indicators for oral HPV carriage in people living with HIV. Data on socio-demographics, sexual behavioral practices, and lifestyle practices of the participants were collected from 66 people living with HIV. The HIV parameters of each study participant were obtained from clinical records. Oral rinses obtained from each participant were subjected to HPV ELISA antigen test for screening and extracted DNA was subjected to nested PCR and whole-genome sequencing for genotyping. Approximately 36% (10 of 28) HIV-positive individuals had oral HPV carriage with one person carrying oncogenic type, HPV16. In addition, 80% (8 of 10) of those with HPV positivity by PCR are females, but with no statistically significant association. The CD4 count showed no significant association with oral HPV carriage in HIV positive individuals; however, age at first sex is a determinant of oral HPV infection in people living with HIV with positive association observed on both bivariate analysis and logistic regression (AOR: 150.49, 95% CI: 1.40-16,155.47, p = 0.036).","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"129-146"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A family cluster of persistent Pandoraea vervacti infection in cystic fibrosis. 囊性纤维化患者持续性vervaci潘多拉菌感染的家族聚集性。

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2025-02-11 DOI: 10.1080/15321819.2025.2462807

Camilla Bitossi, Matteo Fracella, Agnese Viscido, Fabiana Diaco, Claudia Pecoraro, Patrizia Troiani, Giuseppe Cimino, Gabriella d'Ettorre, Maria Trancassini, Alessandra Pierangeli, Guido Antonelli, Carolina Scagnolari

{"title":"A family cluster of persistent Pandoraea vervacti infection in cystic fibrosis.","authors":"Camilla Bitossi, Matteo Fracella, Agnese Viscido, Fabiana Diaco, Claudia Pecoraro, Patrizia Troiani, Giuseppe Cimino, Gabriella d'Ettorre, Maria Trancassini, Alessandra Pierangeli, Guido Antonelli, Carolina Scagnolari","doi":"10.1080/15321819.2025.2462807","DOIUrl":"10.1080/15321819.2025.2462807","url":null,"abstract":"Bacterial colonization of the cystic fibrosis (CF) airways is polymicrobial and several emerging microorganisms with a potential pathogenic role may be present. We report a case of three siblings with CF colonized by Pandoraea over a period of 10 years. Isolates identified as various non-fermentative Gram-negative bacilli from sputum cultures were successfully re-identified as Pandoraea vervacti (P. vervacti) by a combination of matrix-assisted laser desorption ionization - time of flight mass spectrometry (MALDI-TOF MS) and 16S rRNA and gyrB sequencing. Furthermore, the transcript expression of type I and type III interferon (IFN) genes was examined in the cells of respiratory samples from these patients and compared with a Pandoraea-negative group of CF individuals. Increased respiratory levels of IFNβ, IFNε and IL28R1 mRNA were found in the three siblings. Our results demonstrate that P. vervacti can chronically colonize CF patients and alter IFN response, likely contributing to immunopathogenesis and disease progression.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"201-206"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0