Journal of immunoassay & immunochemistry最新文献

Rubella IgG and IgM sero-quantitative profiles of pregnant women attending University College Hospital and Adeoyo Maternity Teaching Hospital in Ibadan, Nigeria. 尼日利亚伊巴丹大学学院医院和Adeoyo妇产教学医院孕妇风疹IgG和IgM血清定量分析

Journal of immunoassay & immunochemistry Pub Date : 2026-04-27 DOI: 10.1080/15321819.2026.2661748

Abdulmujeeb D Opatola, Oluseyi A Olayinka, Esther O Awodire, Emmanuel O Owolabi, Bolutife Popoola, Olubusuyi M Adewumi

{"title":"Rubella IgG and IgM sero-quantitative profiles of pregnant women attending University College Hospital and Adeoyo Maternity Teaching Hospital in Ibadan, Nigeria.","authors":"Abdulmujeeb D Opatola, Oluseyi A Olayinka, Esther O Awodire, Emmanuel O Owolabi, Bolutife Popoola, Olubusuyi M Adewumi","doi":"10.1080/15321819.2026.2661748","DOIUrl":"https://doi.org/10.1080/15321819.2026.2661748","url":null,"abstract":"Rubella virus (RUBV), a contagious viral infection with teratogenic potential, poses a significant risk to pregnant women due to its potential to cause Congenital Rubella Syndrome (CRS). Rubella remains endemic in resource-constrained regions of the world, including Nigeria. This study aimed to determine the seroprevalence of Rubella-specific IgG and IgM antibodies among pregnant women in Ibadan, Oyo State, Nigeria. A cross-sectional study was conducted among 276 consenting pregnant women attending Antenatal Clinics at University College Hospital and Adeoyo Maternity Teaching Hospital in Ibadan. Blood samples were collected and analyzed using IDEAL RV-IgG and RV-IgM ELISA kits to detect Rubella virus-specific antibodies. Socio-demographic data were obtained using structured questionnaires, and statistical analysis was done to test associations at a p-value ≤ 0.05. Overall, 264 (95.65%) participants tested positive for Rubella IgG antibodies, indicating widespread immunity, while 6 (2.17%) tested positive for Rubella IgM antibodies, indicative of recent infection. Significantly, 1 (0.36%) tested positive for Rubella IgM antibodies in her first trimester, suggesting heightened risk of rubella infection and CRS. IgG seropositivity showed a statistically significant association (p < 0.001) with some occupations. Study results show high levels of Rubella immunity among pregnant women in Ibadan, likely due to prior exposure, and confirm recent infections with the detection of IgM antibodies in some of the participants, thus underscoring the urgent need for intervention.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seroprevalence of chikungunya virus infection and co-occurrence with malaria among patients with febrile illness in Oguma, the middle belt region of Nigeria. 尼日利亚中部地带奥古马地区发热性疾病患者中基孔肯雅病毒感染和伴发疟疾的血清患病率

Journal of immunoassay & immunochemistry Pub Date : 2026-04-26 DOI: 10.1080/15321819.2026.2661007

Matthew Jere Daudu, Cornelius Arome Omatola

{"title":"Seroprevalence of chikungunya virus infection and co-occurrence with malaria among patients with febrile illness in Oguma, the middle belt region of Nigeria.","authors":"Matthew Jere Daudu, Cornelius Arome Omatola","doi":"10.1080/15321819.2026.2661007","DOIUrl":"https://doi.org/10.1080/15321819.2026.2661007","url":null,"abstract":"Globally, chikungunya virus infection has become a major public health concern due to the frequent epidemics and increased introduction into new regions. The current study provided a baseline report of chikungunya virus infection and its co-infection in the previously unexplored Oguma, Bassa Local Government Area. Blood samples and relevant sociodemographic variables were obtained from a cross-section of 200 febrile patients. Samples were screened serologically for chikungunya virus antibodies and microscopically for malaria parasites. Overall, anti-chikungunya virus antibodies were detected in 9(4.50%), out of which IgM and IgG were 4(2.0%) and 5(2.5%), respectively. Three (1.5%) of the patients with active chikungunya virus infection were co-infected with malaria, while 1(1.0%) was positive for viral mono-infection. Sixty (30%) patients in the study had malaria alone. Active cases of chikungunya virus infection were observed in males only [4(4.3%) vs 0(0.0%)], who also showed evidence of previous viral infection [3(3.3%) vs 2(1.9%)]. Among the small group of positive cases, rash and back pain were frequently observed alongside active chikungunya virus infection (p < 0.05). Our study confirms the circulation of the chikungunya virus in the unexplored Oguma area, though at a low level when compared to other epidemiological investigations elsewhere in the country.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2026-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR-Cas at a crossroads: from microbial immunity to precision biotechnology. 十字路口的CRISPR-Cas：从微生物免疫到精密生物技术。

Journal of immunoassay & immunochemistry Pub Date : 2026-04-16 DOI: 10.1080/15321819.2026.2658465

Alyaa I Eliwa, Maha M Eldahshan

{"title":"CRISPR-Cas at a crossroads: from microbial immunity to precision biotechnology.","authors":"Alyaa I Eliwa, Maha M Eldahshan","doi":"10.1080/15321819.2026.2658465","DOIUrl":"https://doi.org/10.1080/15321819.2026.2658465","url":null,"abstract":"Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) form RNA-guided adaptive immune systems in bacteria and archaea that mediate sequence-specific defense against invading genetic elements. Beyond their ecological role in restricting bacteriophage infection and horizontal gene transfer (HGT), CRISPR-Cas systems have been repurposed as programmable nucleases, enabling rapid, scalable, and precise genome engineering. Over the past decade, CRISPR platforms, most prominently Cas9, have transformed functional genomics, accelerated target discovery and drug development, and progressed from experimental tools to clinically evaluated gene and cell therapies. In parallel, growing attention has focused on both native and engineered roles of CRISPR-Cas in shaping HGT, plasmid ecology, and antimicrobial resistance (AMR), as AMR continues to expand globally. In this Review, we integrate advances spanning eukaryotic genome editing and prokaryotic antimicrobial applications. We summarize CRISPR-Cas classification and molecular mechanisms, highlighting spacer acquisition, guide RNA biogenesis, target recognition, and nucleic acid cleavage. We then examine how cellular DNA repair pathways influence editing outcomes and discuss strategies to enhance precision. We further review delivery strategies, such as conjugative plasmids, bacteriophages and phagemids, extracellular vesicles, and nanoparticles, together with evolutionary countermeasures encoded by mobile genetic elements, including anti-CRISPR proteins. Finally, we outline current limitations.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-24"},"PeriodicalIF":0.0,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategic analysis of hepatitis B virus serological profile and the diagnostic utility of HBcrAg across Osun and Plateau State, Nigeria. 尼日利亚奥孙州和高原州乙型肝炎病毒血清学特征和HBcrAg诊断效用的战略分析

Journal of immunoassay & immunochemistry Pub Date : 2026-01-01 Epub Date: 2026-03-04 DOI: 10.1080/15321819.2026.2639308

Priscilla Abechi, Uwem E George, Olamide Y Aborisade, Arthur O Oragwa, Oluwatomisin P Osijonwo, Oluremi I Ajayi, Olusola A Ogunsanya, Christian Happi, Onikepe A Folarin

{"title":"Strategic analysis of hepatitis B virus serological profile and the diagnostic utility of HBcrAg across Osun and Plateau State, Nigeria.","authors":"Priscilla Abechi, Uwem E George, Olamide Y Aborisade, Arthur O Oragwa, Oluwatomisin P Osijonwo, Oluremi I Ajayi, Olusola A Ogunsanya, Christian Happi, Onikepe A Folarin","doi":"10.1080/15321819.2026.2639308","DOIUrl":"10.1080/15321819.2026.2639308","url":null,"abstract":"Hepatitis B virus (HBV) infection remains a major concern in Nigeria. Nationally, increasing infection rates continue to pose a significant challenge, which may be due to inadequate diagnosis. Known markers, such as Hepatitis B Surface Antigen (HBsAg) and Hepatitis B e antigen (HBeAg), are good at indicating exposure rates, but generally poor at reflecting active viral replication. The hepatitis B core-related antigen (HBcrAg) may provide a clearer picture of the viral activity and treatment response. We conducted a cross-sectional study involving 450 participants aged 16-65 years from hospitals in Osun and Plateau States. HBsAg screening was performed using rapid diagnostic tests, confirmed by enzyme-linked immunosorbent assay (ELISA), and further evaluated for HBeAg and HBcrAg levels. Correlation analysis was used to test the relationships between these markers. Of the 450 participants screened, 188 (41.7%) were confirmed positive for HBV. HBcrAg was observed in adults aged 17-45 years, suggesting replication activity in symptom-free individuals. Atypical serological profiles, such as HBsAg and HBsAb co-positivity, were also identified. Incorporation of HBcrAg in HBV screening, especially in endemic countries, may be useful in assessing HBV replication stage, reactivation, and staging of chronic infection, especially in resource-limited settings.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"129-151"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic association of Angiopoietin-2 rs12674822 and rs3739390 polymorphisms with systemic lupus erythematosus risk: a preliminary single-center study in Egyptian patients. 血管生成素-2 rs12674822和rs3739390多态性与系统性红斑狼疮风险的遗传关联：埃及患者的初步单中心研究

Journal of immunoassay & immunochemistry Pub Date : 2026-01-01 Epub Date: 2026-03-21 DOI: 10.1080/15321819.2026.2646890

Eman Abd Allah Fouda, Alshimaa M Elmalawany, Eman Masoud Abd El Gayed, Salah Mohammed El-Kousy, Salma Samir Esmail, Mohamed Farag Ali Assar

引用次数: 0

Forensic significance of ELISA technique for hormone assessment: a comprehensive review. ELISA技术对激素评估的法医意义：全面回顾。

Journal of immunoassay & immunochemistry Pub Date : 2026-01-01 Epub Date: 2025-12-20 DOI: 10.1080/15321819.2025.2603249

Radhika Agarwal, Tejasvi Pandey, Deeksha Verma, Arun Kumar

{"title":"Forensic significance of ELISA technique for hormone assessment: a comprehensive review.","authors":"Radhika Agarwal, Tejasvi Pandey, Deeksha Verma, Arun Kumar","doi":"10.1080/15321819.2025.2603249","DOIUrl":"10.1080/15321819.2025.2603249","url":null,"abstract":"Enzyme-linked immunosorbent assay (ELISA) is a rapid, sensitive, and economical tool for detecting hormones in diverse biological matrices, making it valuable in forensic endocrinology. This review summarizes ELISA's principles, applications, advantages, and limitations in medico-legal practice. ELISA enables quantification of hormones such as cortisol, testosterone, estrogen, melatonin, thyroid hormones, progesterone, and human chorionic gonadotropin (hCG). It supports postmortem toxicology, sexual assault investigations, doping control, and forensic psychiatry. Cortisol may help reconstruct perimortem stress, while hCG detection assists in pregnancy confirmation in assault or maternal death cases. In sports, ELISA screens for anabolic steroids, erythropoietin, and growth hormone, with LC-MS/MS required for confirmation. Its compatibility with blood, saliva, urine, and hair enhances versatility. Key challenges include antibody cross-reactivity, matrix interference, degradation, variability among commercial kits, and limited multiplexing. False positives, hook effects, and inconsistent validation affect admissibility, necessitating strict quality assurance, ISO/IEC 17025 compliance, and confirmatory testing. ELISA is unsuitable for paternity determination; DNA profiling remains the legal standard. Emerging advances - digital ELISA, nanotechnology, AI, and biosensors - promise greater sensitivity and automation but face regulatory, cost, and training barriers. ELISA should be regarded as a complementary, high-throughput screening method integrated into validated, multimodal forensic workflows.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-17"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145800051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

pcDNA3.1-rpfB or pcDNA3.1-rpfD recombinant DNA prevents Mycobacterium tuberculosis infection in BALB/c mice. pcDNA3.1-rpfB或pcDNA3.1-rpfD重组DNA对BALB/c小鼠结核分枝杆菌感染有预防作用。

Journal of immunoassay & immunochemistry Pub Date : 2026-01-01 Epub Date: 2026-01-05 DOI: 10.1080/15321819.2025.2611008

Andriansjah Rukmana, Jongga Adiyaksa, Fithriyah Sjatha, Yayi Dwina Billianti, Wahyu Agung Pratama, Eni Nolia

{"title":"pcDNA3.1-rpfB or pcDNA3.1-rpfD recombinant DNA prevents Mycobacterium tuberculosis infection in BALB/c mice.","authors":"Andriansjah Rukmana, Jongga Adiyaksa, Fithriyah Sjatha, Yayi Dwina Billianti, Wahyu Agung Pratama, Eni Nolia","doi":"10.1080/15321819.2025.2611008","DOIUrl":"10.1080/15321819.2025.2611008","url":null,"abstract":"Tuberculosis (TB) is caused by an infection with Mycobacterium tuberculosis. The Bacille Calmette-Guérin (BCG) vaccine is used to treat TB. However, its efficacy varies in different countries. A new TB vaccine is urgently required to inhibit the spread of TB infection. DNA vaccines are promising for providing an immune response against the disease. The resuscitation-promoting factors B and D (rpfB/rpfD) genes are promising vaccine candidates. In this study, the vaccine candidates pcDNA3.1-rpfB and pcDNA3.1-rpfD were evaluated for their ability to inhibit M. tuberculosis infection in BALB/c mice. Epitope analysis indicates that the recombinant protein sequences of RpfB and RpfD used in this study possess epitopes recognized by T and B lymphocytes. Although the presence of M. tuberculosis cells in lung tissue was not detected, histopathological analysis revealed the absence of lymphoid aggregates in mice vaccinated with pcDNA3.1-rpfB or pcDNA3.1-rpfD, in contrast to those administered with phosphate-buffered saline or pcDNA3.1. In addition, analysis of the humoral immune response showed the highest IgG2a antibody titer in mice immunized with both vaccine candidates. These results support our previous findings, which indicate that pcDNA3.1-rpfB and pcDNA3.1-rpfD have considerable potential as TB vaccine candidates.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"77-95"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145906014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the therapeutic potential of syringaldehyde in imiquimod-induced psoriasis model in mice via NF-κB pathway inhibition. 通过抑制NF-κB通路探讨丁香醛对吡喹莫德诱导的小鼠银屑病模型的治疗潜力。

Journal of immunoassay & immunochemistry Pub Date : 2026-01-01 Epub Date: 2025-11-06 DOI: 10.1080/15321819.2025.2584190

Meeral Gosia, Angel Godad, Akshata Pahelkar, Gaurav Doshi

{"title":"Exploring the therapeutic potential of syringaldehyde in imiquimod-induced psoriasis model in mice via NF-κB pathway inhibition.","authors":"Meeral Gosia, Angel Godad, Akshata Pahelkar, Gaurav Doshi","doi":"10.1080/15321819.2025.2584190","DOIUrl":"10.1080/15321819.2025.2584190","url":null,"abstract":"Psoriasis is characterized by increased levels of pro-inflammatory cytokines, including TNF-α, IL-1β, IL-17A, and NF-κB, as well as keratinocyte hyperproliferation and epidermal thickening. Its pathophysiology is significantly influenced by oxidative stress and abnormal activation of redox-sensitive signaling pathways, such as NF-κB and MAPK. The present study examined the potential of Syringaldehyde (SYD) in the imiquimod (IMQ) induced psoriasis model. Psoriasis Area Severity Index (PASI) scores, back skin thickness, and spleen hypertrophy decreased at dose levels of SYD 25, 50, and 100 mg/kg. Furthermore, a significant reduction in PASI scores following SYD administration indicated a marked improvement in the disease severity and lesion morphology. High-affinity binding of SYD to NF-κB (PDB ID: 4KIK; -34.76 kcal/mol) and MAPK (PDB ID: 1A9U; -32.87 kcal/mol) was found by molecular docking, indicating interference with nuclear translocation and phosphorylation processes. The treatment groups 50 mg/kg and 100 mg/kg indicated restoration of normal histological features. The biochemical evaluation showed decrease in NF-κB, IL-1β, TNF-α, and IL-17 on treatment with SYD. Thus, SYD appears to be a potential therapeutic option for psoriasis, but additional research is needed to confirm its efficacy and safety.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"33-59"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145452144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The association between single-nucleotide polymorphisms of FDPS Rs2297480 and MECP2 Rs2734647 genes and the susceptibility to rheumatoid arthritis in Egyptian patients. 埃及患者FDPS Rs2297480和MECP2 Rs2734647基因单核苷酸多态性与类风湿关节炎易感性的关系

Journal of immunoassay & immunochemistry Pub Date : 2026-01-01 Epub Date: 2026-02-14 DOI: 10.1080/15321819.2026.2624657

Nesreen G Elhelbawy, Basant F Saad, Ibrahim El Tantawy El Sayed, Bishoy El-Aarag, Marwa M Khalil

{"title":"The association between single-nucleotide polymorphisms of FDPS Rs2297480 and MECP2 Rs2734647 genes and the susceptibility to rheumatoid arthritis in Egyptian patients.","authors":"Nesreen G Elhelbawy, Basant F Saad, Ibrahim El Tantawy El Sayed, Bishoy El-Aarag, Marwa M Khalil","doi":"10.1080/15321819.2026.2624657","DOIUrl":"10.1080/15321819.2026.2624657","url":null,"abstract":"Background: Rheumatoid arthritis (RA) has a complex etiology that involves environmental and genetic variables. The identification of new genetic connections contributes to accelerating the development of customized medications to treat or delay the progression of diseases.Objective: We aimed to assess the relationship between rheumatoid arthritis susceptibility and genetic variations of the Farnesyl Diphosphate Synthase (FDPS) and methyl CpG binding protein 2 (MECP2) genes.Patients and methods: 100 patients with rheumatoid arthritis and 100 healthy control subjects participated in this case-control research. Genetic analyses for single-nucleotide polymorphisms (SNPs) in the FDPS and MeCP2 genes were performed on the patients.Results: In terms of FDPS genetic polymorphisms, the sick group had greater frequencies of the TG and GG genotypes, whereas the control group had a higher frequency of the TT genotype. The CC genotype was more common in the control group, but the TC and TT genotypes were more common in the sick group concerning MECP2 genetic polymorphisms.Conclusion: For rheumatoid arthritis, the FDPS (rs2297480) TT genotype seemed to be protective. An increased risk of rheumatoid arthritis was linked to the MECP2 (rs2734647) TT genotype, but the CC genotype seemed to be protective against the condition.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"97-112"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146197774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of antimicrobial peptide Human β Defensin-2 with clue cell appearance and the type of culture isolate in women with spontaneous and recurrent pregnancy losses. 抗微生物肽人β防御素-2与自发性和复发性流产妇女线索细胞形态和培养分离物类型的相关性

Journal of immunoassay & immunochemistry Pub Date : 2026-01-01 Epub Date: 2025-08-21 DOI: 10.1080/15321819.2025.2549270

Hind M Al-Khazrajy, Siham Sh Al-Salihi

{"title":"Correlation of antimicrobial peptide Human β Defensin-2 with clue cell appearance and the type of culture isolate in women with spontaneous and recurrent pregnancy losses.","authors":"Hind M Al-Khazrajy, Siham Sh Al-Salihi","doi":"10.1080/15321819.2025.2549270","DOIUrl":"10.1080/15321819.2025.2549270","url":null,"abstract":"Human β Defensin-2 (HBD2) is an Antimicrobial peptide (AMP) that serves a dual function in host defense and fertility. On the other hand, a plethora of microbial infectious agents have been associated with an increased risk of spontaneous abortion (SA). This study examined the impact of HBD-2 on women with SA with a particular focus on its relationship with clue cells and other microbial infections. 100 women aged 17-50 with a history of SA participated. We utilized two vaginal swabs. They were cultivated first. The Vitek2 technique was used after biochemical tests identified the isolates. vaginosis diagnosed by Amsel criteria. Serum HBD-2 levels were measured using Enzyme-linked immunosorbent assay (ELISA). An association was observed between bacterial vaginosis and SA in the second trimester. HBD-2 levels fell with age. Women with five or more abortions had lower HBD-2 levels (p = 0.034). In cases containing clue cells, HBD-2 averaged 586.0 pg/mL, whereas in cases without clue cells, it decreased (p = 0.007). Gram-negative bacterial infections decreased HBD-2. During fungal infection, HBD-2 levels dropped to 176.1 pg/ml. A significant relationship was observed between HBD2 levels and vaginitis, suggesting its potential as a biomarker and diagnostic tool for reproductive health.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"18-32"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0