Journal of immunoassay & immunochemistry最新文献

An indirect competitive ELISA assay using fragment antigen-binding (Fab) antibody for the quantitative detection of delta-9-tetrahydrocannabinol from Cannabis sativa L. 采用片段抗原结合抗体（Fab）间接竞争ELISA法定量检测大麻中δ -9-四氢大麻酚。

Journal of immunoassay & immunochemistry Pub Date : 2025-07-27 DOI: 10.1080/15321819.2025.2538777

Worapol Sae-Foo, Jakkapat Paluka, Pewpan Maleewong Intapan, Waraporn Putalun

{"title":"An indirect competitive ELISA assay using fragment antigen-binding (Fab) antibody for the quantitative detection of delta-9-tetrahydrocannabinol from Cannabis sativa L.","authors":"Worapol Sae-Foo, Jakkapat Paluka, Pewpan Maleewong Intapan, Waraporn Putalun","doi":"10.1080/15321819.2025.2538777","DOIUrl":"https://doi.org/10.1080/15321819.2025.2538777","url":null,"abstract":"Delta-9-tetrahydrocannabinol (THC) is one of the cannabinoid metabolites present in Cannabis sativa. Currently, cannabis is an ingredient in foods, cosmetics, and medicinal products. Many countries control cannabis and THC for illegal or under-regulation products. This study focused on development of recombinant antibodies specific to THC, and their application of analytical methods using enzyme-linked immunosorbent assay (ELISA) techniques. Conjugation of THC with bovine serum albumin or ovalbumin was performed using Mannich and N,N'-carbonyldiimidazole reactions. The recombinant antibody was expressed from Escherichia coli in form of fragmented antigen-binding (Fab) antibody. Antigen-conjugates and Fab were performed using ELISA-based analytical method and validated method for sensitivity, accuracy, and precision. The developed Fab in this study demonstrates high specificity for detection of THC. The analytical range was between 0.78 and 25 µg/mL, with limit of detection of 0.59 µg/mL. Accuracy ranged from 98.15-108.97%, with intra- and inter-assay % relative standard deviation of 2.15-8.03 and 1.83-8.45%, respectively. Parameters for method validation, including accuracy and precision, were within acceptable limits which are comparable to modified HPLC method from previous study. The approach demonstrated outstanding sensitivity and specificity for analysis of THC in cannabis extracts, suggesting it to be suitable for the quantitative assessment of THC in variety of products.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-22"},"PeriodicalIF":0.0,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the impact of interferon-ß and rituximab on NLRP3 and AIM2 expression and IL-1β levels in patients with multiple sclerosis. 评估干扰素β和利妥昔单抗对多发性硬化症患者NLRP3和AIM2表达及IL-1β水平的影响。

Journal of immunoassay & immunochemistry Pub Date : 2025-07-17 DOI: 10.1080/15321819.2025.2534453

Sina Molavizade, Fereshteh Ashtari, Fateme Dehghani, Zahra Karimi, Mohammad Mahjoubi, Nasrin Zare

{"title":"Assessment of the impact of interferon-ß and rituximab on NLRP3 and AIM2 expression and IL-1β levels in patients with multiple sclerosis.","authors":"Sina Molavizade, Fereshteh Ashtari, Fateme Dehghani, Zahra Karimi, Mohammad Mahjoubi, Nasrin Zare","doi":"10.1080/15321819.2025.2534453","DOIUrl":"https://doi.org/10.1080/15321819.2025.2534453","url":null,"abstract":"Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system. Inflammasomes, particularly NLRP3 and AIM2, have been implicated in MS pathogenesis. Interferon-β (IFN-β) and Rituximab (RTX) are treatment agents for relapsing-remitting MS (RRMS), but their impact on inflammasome regulation remains unclear. This study evaluates the expression of NLRP3 and AIM2 inflammasomes in MS patients responding to IFN-β and RTX, alongside newly diagnosed cases and healthy controls. Blood samples from IFN-β-treated patients (n = 23), RTX-treated patients (n = 23), newly diagnosed people (n = 20), and healthy controls (n = 12) were analysed. mRNA levels of NLRP3 and AIM2 were measured by RT-PCR, and plasma IL-1β was assessed using ELISA. Results revealed AIM2 expression was significantly higher in RTX-treated patients compared to other groups, while NLRP3 showed no significant differences. IL-1β levels were elevated in all patient groups, but no correlations were found between disease/treatment duration and inflammasome or IL-1β levels. RTX significantly increases AIM2 expression, suggesting its potential as a biomarker or therapeutic target in MS. Further research is needed to clarify AIM2's role in disease processes.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-17"},"PeriodicalIF":0.0,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of SHMT2 and NDUFA4L2 gene expression and UCA1 levels in Egyptian patients with bladder cancer. SHMT2和NDUFA4L2基因表达和UCA1水平在埃及膀胱癌患者中的作用

Journal of immunoassay & immunochemistry Pub Date : 2025-07-14 DOI: 10.1080/15321819.2025.2533800

Eman A E Badr, Yostena Mekhail, Khaled S A Hassan, Mohamed F A Assar

{"title":"The role of SHMT2 and NDUFA4L2 gene expression and UCA1 levels in Egyptian patients with bladder cancer.","authors":"Eman A E Badr, Yostena Mekhail, Khaled S A Hassan, Mohamed F A Assar","doi":"10.1080/15321819.2025.2533800","DOIUrl":"https://doi.org/10.1080/15321819.2025.2533800","url":null,"abstract":"Bladder Cancer (BC) has a significant burden, with poor survival rates in advanced cases. This case-controlled study aimed to identify genes having diagnostic and prognostic value. Seventy patients with BC and 70 healthy individuals were included. Real-time PCR was used to quantify the expression of the SHMT2 and NDUFA4L2 genes, and UCA1 levels. Results showed that SHMT2, NDUFA4L2, and UCA1 were significantly upregulated in patients compared to the control group (p < 0.001). The area under the receiver operating characteristic curve (ROC) analysis showed that AUC was 0.995 (95% CI = 0.989-1, p < 0.001), 0.994 (95% CI = 0.987-1, p < 0.001) and 0.977 (95% CI = 0.958-0.996, p < 0.001) for UCA1, SHMT2, and NDUFA4L2, respectively with high diagnostic sensitivity and specificity. Additionally, the high expression of SHMT2 and the high level of UCA1 were correlated with histological grade (p < 0.001and 0.001, respectively) and with CT tumor size (p = 0.005 and 0.001, respectively). In univariate COX regression analysis, SHMT2 and UCA1 were poor prognostic (p = 0.003 and 0.001, respectively). Furthermore, in the multivariate analysis, UCA1 was independent poor prognostic factor (p =0.037). Thus, SHMT2, NDUFA4L2, and UCA1 could be promising diagnostic and prognostic biomarkers and a novel therapeutic target.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-chain variable fragment antibodies targeting the antileukemia agent harringtonine for enzyme-linked immunosorbent assay development. 单链可变片段抗体靶向抗白血病药物哈林丁碱酶联免疫吸附试验的发展。

Journal of immunoassay & immunochemistry Pub Date : 2025-07-09 DOI: 10.1080/15321819.2025.2526208

Seiichi Sakamoto, Shohei Komatsu, Ai Moriyasu, Gorawit Yusakul, Waraporn Putalun, Poomraphie Nuntawong, Hiroyuki Tanaka, Satoshi Morimoto

{"title":"Single-chain variable fragment antibodies targeting the antileukemia agent harringtonine for enzyme-linked immunosorbent assay development.","authors":"Seiichi Sakamoto, Shohei Komatsu, Ai Moriyasu, Gorawit Yusakul, Waraporn Putalun, Poomraphie Nuntawong, Hiroyuki Tanaka, Satoshi Morimoto","doi":"10.1080/15321819.2025.2526208","DOIUrl":"https://doi.org/10.1080/15321819.2025.2526208","url":null,"abstract":"In this study, we generated a single-chain variable fragment (scFv) specific to a potent antileukemic substance, harringtonine (HT) (HT-scFv) that can be used in enzyme-linked immunosorbent assay (ELISA) for the quantitative analysis of HT in the plant genus Cephalotaxus. The variable heavy (VH) and light chain (VL) genes were directly cloned from the cDNA of the hybridoma cell line 1D2, which secretes monoclonal antibody against HT (MAb 1D2). These genes were then assembled with a flexible peptide linker, specifically (Gly4Ser)3, through splicing by overlap extension PCR. The resulting HT-scFv gene was expressed in Escherichia coli. The denatured HT-scFv, produced as inclusion bodies, was solubilized and refolded using a dilution method to restore its functionality as an antibody. Characterization of the HT-scFv demonstrated its high specificity for HT. Additionally, its remarkable properties facilitated the development of an ELISA for HT detection, achieving a limit of detection (LOD) of 12.2 ng/mL. Furthermore, validation analyses showed that the ELISA using HT-scFv exhibited good accuracy and reliability for the quantitative assessment of HT in C. harringtonia \"Fastigiata.\" This study highlights the potential of HT-scFv as a valuable tool for ELISA in the quantitative analysis of HT.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occult hepatitis B and the detection of other HBV genes in HBs gene negative individuals in southwest Nigeria. 尼日利亚西南部HBs基因阴性个体隐匿性乙型肝炎及其他HBV基因的检测

Journal of immunoassay & immunochemistry Pub Date : 2025-06-12 DOI: 10.1080/15321819.2025.2516487

Olufisayo A Adesina, Favour A Babarinde, Dapo J Oluwajuyite, Oluwawemimo T Akinlabi

{"title":"Occult hepatitis B and the detection of other HBV genes in HBs gene negative individuals in southwest Nigeria.","authors":"Olufisayo A Adesina, Favour A Babarinde, Dapo J Oluwajuyite, Oluwawemimo T Akinlabi","doi":"10.1080/15321819.2025.2516487","DOIUrl":"https://doi.org/10.1080/15321819.2025.2516487","url":null,"abstract":"Considering the high tendency of hepatitis B virus (HBV) to mutate and escape diagnosis, and the high prevalence of its chronic infection in Nigeria, it is expedient to wholistically study the various genes to understand the changes and to prepare for such to enhance the diagnosis, management, and control of the disease. This study was designed to detect the four genes of HBV in different categories of participants. Venous blood samples were collected from sick and apparently healthy individuals and screened for HBsAg using the ELISA method. Out of the samples, 36 hBsAg positive and 9 randomly selected HBsAg negative were selected for HBV DNA extraction and PCR using established primers and protocols. The HBsAg prevalence was found to be 13.8% (51/369) with Oyo state having the highest (37.3%; N = 19/51) compared to other states. After amplification, HBs gene detection was 28.9%; (n = 13/45), HBc gene 33.3%; (n = 15/45), HB Pol gene 26.7%; (n = 12/45), and HBx gene 35.6%; (n = 16/45) and one OBI case (11.1%; n = 1/9). Considering the complications associated to HBV infections and the various changes being observed in the structure of the virus, more study is needed to enhance the diagnosis, manage- 230 ment, and control of hepatitis B.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of analysis of serum free light chains in a cartridge-based protein analyzer Mispa i3 with Roche Cobas 8000. Mispa i3蛋白分析仪与罗氏Cobas 8000蛋白分析仪血清游离轻链分析的比较

Journal of immunoassay & immunochemistry Pub Date : 2025-05-08 DOI: 10.1080/15321819.2025.2502433

Abhijith B L, Thushara Thomas, Dinimol Daniel, Jofy K Paul, D M Vasudevan

{"title":"Comparison of analysis of serum free light chains in a cartridge-based protein analyzer Mispa i3 with Roche Cobas 8000.","authors":"Abhijith B L, Thushara Thomas, Dinimol Daniel, Jofy K Paul, D M Vasudevan","doi":"10.1080/15321819.2025.2502433","DOIUrl":"https://doi.org/10.1080/15321819.2025.2502433","url":null,"abstract":"Serum-free light-chain assays are important in the diagnosis and in monitoring therapeutic responses of plasma cell disorders and are complementary to serum protein electrophoresis. The serum-free light-chain assay detects the light-chain portion of immunoglobulin in its free form with high sensitivity. In combination with serum protein electrophoresis and serum immunofixation electrophoresis, the free light-chain assay serves an important role in predicting disease progression in monoclonal gammopathy. Here, we compare the performance of a cartridge-based system Mispa i3 using Diazyme reagent in comparison to Roche Cobas using Freelite reagent. Both of these reagents use polyclonal antibodies for the detection of serum-free light chains. Mispa i3 is a cartridge-based protein analyzer and has a unique channel shifting technology with both turbidimetric and nephelometric principles for immuno assays. Samples of 196 patients were included in this study, and very good agreement was observed between these two assays. Our data show that even though discrepancies were observed for high concentration samples, they are clinically correlated by the free light-chain ratios. We observed a very good concordance of 89% between these two assays for free light-chain ratios.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Costus speciosus extract: a natural immunomodulator for treatment of psoriasis in BALB/c mice by NF-κB pathway. 木香提取物：经NF-κB通路治疗BALB/c小鼠银屑病的天然免疫调节剂

Journal of immunoassay & immunochemistry Pub Date : 2025-05-02 DOI: 10.1080/15321819.2025.2498439

Aditi Vyas, Muqtada Shaikh, Radhika Raheja, Gaurav Doshi

{"title":"Costus speciosus extract: a natural immunomodulator for treatment of psoriasis in BALB/c mice by NF-κB pathway.","authors":"Aditi Vyas, Muqtada Shaikh, Radhika Raheja, Gaurav Doshi","doi":"10.1080/15321819.2025.2498439","DOIUrl":"https://doi.org/10.1080/15321819.2025.2498439","url":null,"abstract":"Psoriasis is a chronic inflammatory skin condition. Ayurveda, Traditional Chinese therapy, and Unani offer a potential approach to treating psoriasis. This study explores the immunomodulatory effects of Costus speciosus petroleum ether extract (CSPE) on psoriasis-like symptoms in Balb/c mice. GC-MS analysis was done to detect bioactive phytoconstituents in the extract. Mice were treated with Imiquimod (IMQ) to induce psoriasis-like symptoms. Measurements of back skin thickness, skin length, mass, and body weight were used in the study, with an assessment of the Psoriasis Area and Severity Index (PASI). The treatment group received doses of 110 mg/kg, 220 mg/kg, and 440 mg/kg of extracts. Nuclear Factor kappa-B (NF-kB), tumor necrosis factor-alpha (TNF-α), and interleukin-17 (IL-17) were estimated. Presence of diosgenin, tigogenin, aglycone of diosgenin, santamarine and reynosin in the extract was confirmed by GC-MS analysis. At 440 mg/kg, a significant effect was observed in the IMQ model. The extract significantly reduced levels of NF-kB, TNF-α, and IL-17. The research findings indicate that CSPE is a promising candidate for psoriasis treatment.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-21"},"PeriodicalIF":0.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Burcu Kök Kendirlioğlu, MD, PhD -Early Career Prize Winner 2024. Burcu Kök Kendirlioğlu，医学博士，博士- 2024年早期职业奖得主。

Journal of immunoassay & immunochemistry Pub Date : 2025-04-20 DOI: 10.1080/15321819.2025.2496044

引用次数: 0

Evaluation of Ki67 in pathological prognostic staging of breast cancer: a tertiary care center study. Ki67在乳腺癌病理预后分期中的评价：一项三级保健中心研究。

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2025-01-15 DOI: 10.1080/15321819.2025.2451211

Rashmi Wankhade, Arvind Bhake, Nandkishor Bankar, Yugeshwari Tiwade

{"title":"Evaluation of Ki67 in pathological prognostic staging of breast cancer: a tertiary care center study.","authors":"Rashmi Wankhade, Arvind Bhake, Nandkishor Bankar, Yugeshwari Tiwade","doi":"10.1080/15321819.2025.2451211","DOIUrl":"10.1080/15321819.2025.2451211","url":null,"abstract":"Background: The rising global burden of breast cancer demands early detection and effective treatment, with a focus on prognostic and predictive markers. The eighth edition of the American Joint Committee on Cancer staging manual introduced a new prognostic staging system to increase the predictive power of the existing anatomical staging system of breast cancer. The current study aimed to establish the correlation between Ki67 expression with molecular subtypes and with the pathological prognostic stage of invasive ductal carcinoma.Materials and methods: A total of 40 patients were included in the study with samples from 32 modified radical mastectomies and 8 biopsies. Hematoxylin and Eosin staining, histopathological analysis and Ki67 immunostaining were conducted. Descriptive and inferential statistical analyses were performed.Results: Bloom Richardson Grade II was the predominant histological grade. In Grade II cases, 15 of 24 had a Ki67 labeling index of 26-45%, while 6 exceeded 45% (p = 0.001). Pathological prognostic staging reclassified 27 cases, with 24 (75%) downstaged, 3 (9.38%) upstaged, and 5 (15.63%) retaining their clinical stage.Conclusions: Ki67 immunohistochemistry is an effective tool for assessing proliferative activity of invasive ductal carcinoma, aiding in pathological prognostic stage stratification and offering insights into tumor biology.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"186-200"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparison between different chemical fractionation methods for immunoglobulin preparation. 制备免疫球蛋白不同化学分馏方法的比较。

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2025-01-11 DOI: 10.1080/15321819.2025.2450664

Abbasali Salarifar, Pooria Safarzadeh Kozani, Mohammad Javad Rasaee

{"title":"A comparison between different chemical fractionation methods for immunoglobulin preparation.","authors":"Abbasali Salarifar, Pooria Safarzadeh Kozani, Mohammad Javad Rasaee","doi":"10.1080/15321819.2025.2450664","DOIUrl":"10.1080/15321819.2025.2450664","url":null,"abstract":"Background: Application of antibodies in therapeutics and diagnostics are growing Continually. Herein, we aimed to find the most qualified immunoglobulin (Ig) chemical preparation method.Methods: A rabbit was immunized against recombinant SARS-CoV-2 nucleocapsid (NP) and reactive polyclonal antibodies were prepared using the ammonium sulfate (AS), caprylic acid (CA), polyethylene glycol (PEG), and caprylic acid/ammonium sulfate (CA/AS) methods. Different antibody solutions were analyzed by SDS-PAGE and subsequently quantified by ImageJ software for further analysis in terms of Ig purity, Ig recovery, and albumin impurity. Ultimately, the prepared antibodies were assessed via Western blotting and ELISA to evaluate their ability to bind NP.Results: Prepared Ig solutions via the CA/AS method had the highest Ig purity (followed by CA, PEG, and AS) and lowest albumin impurity (followed by CA, AS, and PEG). The PEG method had the highest recovery followed by AS, CA, and CA/AS methods. Moreover, antibodies prepared via different methods demonstrated comparable binding capacities to NP in ELISA and Western blotting.Conclusions: CA/AS, closely followed by CA, proved to be the most qualified method for the preparation of Ig yielding the highest Ig purity while the PEG method resulted in the highest Ig recovery rate.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"169-185"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0