Journal of immunoassay & immunochemistry最新文献

BRAF V600E protein detection by immunohistochemistry in metastatic colorectal cancer tissue: association with tumor characteristics and KRAS mutation.

Journal of immunoassay & immunochemistry Pub Date : 2025-03-26 DOI: 10.1080/15321819.2025.2484408

Sarra Ben Rejeb, Safia Sakly, Rym Bahloul, Adnen Chouchen

{"title":"BRAF V600E protein detection by immunohistochemistry in metastatic colorectal cancer tissue: association with tumor characteristics and KRAS mutation.","authors":"Sarra Ben Rejeb, Safia Sakly, Rym Bahloul, Adnen Chouchen","doi":"10.1080/15321819.2025.2484408","DOIUrl":"https://doi.org/10.1080/15321819.2025.2484408","url":null,"abstract":"Immunohistochemistry (IHC) using the BRAF V600E antibody is a sensitive and specific method for detecting BRAF V600E mutations in colorectal cancer (CRC). Given that BRAF and KRAS mutations are mutually exclusive, this study aimed to assess the expression of BRAF V600E in CRC according to KRAS mutation status. Automated IHC analysis was performed on tissue samples from metastatic CRC patients diagnosed between 2012 and 2022 using the anti-BRAF V600E antibody (GenomeME, IHC600). Positive and negative control tissues were used for validation. Cytoplasmic staining was considered positive, with intensity classified as weak, moderate, or strong. The percentage of positive tumor cells was recorded semi-quantitatively. Thirty-five cases were included.The mean age of patients was 60 years (±12.41), with a male-to-female ratio of 2.9. KRAS mutations were present in 48% of cases. BRAF V600E cytoplasmic staining was observed in 37.1%, with a mean percentage of positive cells of 50% (range: 5%-100%). Diffuse and focal staining were found in 7/13 and 6/13 cases, respectively. Significant associations were observed with mucinous carcinoma subtype, invasion patterns, and lymph node metastasis. All KRAS-mutated cases showed negative BRAF staining. Complete absence of BRAF V600E IHC staining in CRC is strongly associated with KRAS mutation, suggesting IHC as an efficient tool to predict KRAS mutational status.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of honey-ginger mouthwash on oral mucositis in patients undergoing chemotherapy.

Journal of immunoassay & immunochemistry Pub Date : 2025-03-25 DOI: 10.1080/15321819.2025.2484645

Fatemeh Sadat Razavi, Hania Zokaee, Mojtaba Sehat, Mohsen Taghizadeh, Hossein Motedayyen, Elaheh Ghasemzadeh Hoseini

{"title":"Effect of honey-ginger mouthwash on oral mucositis in patients undergoing chemotherapy.","authors":"Fatemeh Sadat Razavi, Hania Zokaee, Mojtaba Sehat, Mohsen Taghizadeh, Hossein Motedayyen, Elaheh Ghasemzadeh Hoseini","doi":"10.1080/15321819.2025.2484645","DOIUrl":"https://doi.org/10.1080/15321819.2025.2484645","url":null,"abstract":"Background: Oral mucositis is considered as one of the most prevalent complications of chemotherapy or radiation therapy in cancerous tumors, which can interrupt the patient's treatment and nutrition. This study therefore aimed to evaluate the efficacy of ginger-honey mouthwash on the prevention of chemotherapy-induced oral mucositis in patients suffering from various cancers.Materials and methods: In this randomized clinical trial study, 70 patients receiving chemotherapy were divided into case and control groups. The former group (n = 34) received natural honey-ginger mouthwash and the latter (n = 36) used normal saline for 14 days. The presence and severity of oral mucositis, pain intensity, and other related characteristics were evaluated based on a two-part questionnaire (demographic and clinical information) and a checklist prepared from the protocols of the World Health Organization in each group.Results: During a 14-day intervention, patients received a 7-day intervention with ginger-honey mouthwash revealed a significant reduction in the mean severity of oral mucositis compared to the control group (p = 0.03). However, a 14-day intervention with ginger-honey mouthwash indicated no significant impact on the mean severity of oral mucositis (p = 0.6). In addition, no significant difference was observed in pain intensity between case and control groups during these 14 days.Conclusions: This study suggests that a seven-day intervention with ginger-honey mouthwash has a beneficial effect on reducing the severity of mucositis in patients under chemotherapy, unlike a 14-day intervention. The honey-ginger mouthwash fails to have a significant effect on the pain intensity due to mucositis in patients undergoing chemotherapy.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CXCR4 expression in immunohistochemistry of gastrointestinal neuroendocrine neoplasms: a meta-analysis.

Journal of immunoassay & immunochemistry Pub Date : 2025-03-24 DOI: 10.1080/15321819.2025.2482642

Changlong Ruan, Jiajia Zhang, Yingying Liao, Yujie Hang, Yuan Dong, Hanfeng Shi, Xiaoya Wang

{"title":"CXCR4 expression in immunohistochemistry of gastrointestinal neuroendocrine neoplasms: a meta-analysis.","authors":"Changlong Ruan, Jiajia Zhang, Yingying Liao, Yujie Hang, Yuan Dong, Hanfeng Shi, Xiaoya Wang","doi":"10.1080/15321819.2025.2482642","DOIUrl":"https://doi.org/10.1080/15321819.2025.2482642","url":null,"abstract":"Objective: C-X-C chemokine receptor type 4 (CXCR4) is significantly associated with the development of various malignant tumors. This meta-analysis aims to investigate the expression of CXCR4 in the immunohistochemistry of gastrointestinal neuroendocrine neoplasms (GI-NENs).Methods: A comprehensive literature search regarding gastrointestinal neuroendocrine neoplasms and CXCR4 was conducted using PubMed,Web of Science, and the Cochrane Library, with a cut off date of June 30, 2024.Two researchers independently screened the literature according to inclusion and exclusion criteria and assessed study quality using the Newcastle-OttawaScale. Meta-analysis was performed using Stata version 17.0. The pooled positive rate was employed to evaluate the expression of CXCR4 in the immunohistochemistry of GI-NENs.Results: A total of eight studies involving 501 patients were included in this research. The immunohistochemical analysis revealed positive CXCR4 expression in 174 patients. The meta-analysis calculated and summarized the combined positive rate (R: 0.41; 95% CI = 0.21-0.60, p = 0.00), indicating that the differences were statistically significant.Conclusion: CXCR4 is highly expressed in the immunohistochemistry of GI-NENs, which may provide some evidence for the therapeutic application of CXCR4 antagonists in treating neuroendocrine neoplasms.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SARS-CoV-2 nucleocapsid detection using a recombinant phage display-isolated single-chain fragment variable.

Journal of immunoassay & immunochemistry Pub Date : 2025-03-24 DOI: 10.1080/15321819.2025.2483839

Abbasali Salarifar, Mohammad Javad Rasaee

{"title":"SARS-CoV-2 nucleocapsid detection using a recombinant phage display-isolated single-chain fragment variable.","authors":"Abbasali Salarifar, Mohammad Javad Rasaee","doi":"10.1080/15321819.2025.2483839","DOIUrl":"https://doi.org/10.1080/15321819.2025.2483839","url":null,"abstract":"Background: Diagnosis is an important factor in controlling disease. Single-chain fragment variables (scFvs) can be used for diagnosis; however, due to their immobilization issues, their application has been limited. Herein, we isolated a SARS-CoV-2 nucleocapsid phosphoprotein (NP)-specific scFv and propose it as a diagnostic tool in the scFv-displaying phage format to overcome the immobilization issue.Method: Spleen from NP-immunized BALB/c mice was isolated, total RNA was extracted, and cDNA was synthesized. An scFv library was constructed, using the splicing by overlap extension (SOE) PCR technique, which was cloned into the pCANTAB5E phagemid. The phage library was panned against the NP antigen, and the output phages with the highest binding capability were screened for the most qualified scFv, which was later assessed in terms of sensitivity and specificity.Results: The scFv-displaying phage library was panned against the recombinant NP in three rounds and 40 randomly selected colonies from the third round's outputs were screened. Alongside several clones, clone #31 was chosen as the most qualified scFv, which later exhibited favorable sensitivity and specificity against NP in further ELISA-based experiments.Conclusions: Clone #31 could be utilized to develop diagnostic tools and therapeutics against SARS-CoV-2.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-19"},"PeriodicalIF":0.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of IL2-330 (rs2069762) gene polymorphism in Egyptian patients with lung cancer: a case-control study.

Journal of immunoassay & immunochemistry Pub Date : 2025-03-18 DOI: 10.1080/15321819.2025.2477605

Esraa Tawfik Allam, Mohamed S El Senbawy, Hanaa M Elgazzar, Inas Moaz, Sara Ahmed Saied, Hind S AboShabaan, Shimaa Mohammed Hamad, Samah Ahmed Elbeltagy, Omnia S Nabih

{"title":"Study of IL2-330 (rs2069762) gene polymorphism in Egyptian patients with lung cancer: a case-control study.","authors":"Esraa Tawfik Allam, Mohamed S El Senbawy, Hanaa M Elgazzar, Inas Moaz, Sara Ahmed Saied, Hind S AboShabaan, Shimaa Mohammed Hamad, Samah Ahmed Elbeltagy, Omnia S Nabih","doi":"10.1080/15321819.2025.2477605","DOIUrl":"https://doi.org/10.1080/15321819.2025.2477605","url":null,"abstract":"Background: IL2 is one of the key cytokines essential for regulating the immune system and the inflammation-related carcinogenesis process. Few studies have examined the relationship between lung cancer and the IL2-330 (rs2069762) gene polymorphism, despite several studies demonstrating that it is linked to numerous cancer types.Objective: Our study aimed to investigate the association between IL2-330 (rs2069762) polymorphism and lung cancer risk and explore the role of IL2-330 polymorphism in survival outcomes (OS and PFS).Patients and methods: The study was conducted from October 2023 to November 2024, including 50 randomly selected patients diagnosed with lung cancer and 50 subjects matched for age and gender were used as controls in this case-control study. The IL2-330 (rs2069762) gene polymorphism was assessed using real-time PCR.Results: We found that the AC genotype was associated with a notably lower risk of lung cancer in comparison to the AA genotype (95% CI: 0.06-0.61, p = 0.01). Conversely, the CC genotype showed no significant association with lung cancer risk when compared to the reference genotype. The comprehensive comparison of survival distributions among the AA, AC, and CC genotypes through the Log-Rank (Mantel-Cox) test indicated no statistically significant difference.Conclusions: According to our research, The AC genotype of IL2-330 (rs2069762) is associated with a significantly higher survival rate and lower risk of lung cancer. Further future studies are needed to confirm these findings.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High prevalence of antinuclear antibodies in hepatitis C related hepatocellular carcinoma.

Journal of immunoassay & immunochemistry Pub Date : 2025-03-17 DOI: 10.1080/15321819.2025.2480368

Mohamed El-Far, Ahmed S Mustafa, AbdelfattahM Attallah, Mohamed A Abdelrazek

{"title":"High prevalence of antinuclear antibodies in hepatitis C related hepatocellular carcinoma.","authors":"Mohamed El-Far, Ahmed S Mustafa, AbdelfattahM Attallah, Mohamed A Abdelrazek","doi":"10.1080/15321819.2025.2480368","DOIUrl":"https://doi.org/10.1080/15321819.2025.2480368","url":null,"abstract":"In chronic hepatitis C (CHC), the virus may induce autoimmune responses via autoantibodies production, including antinuclear antibodies (ANA). Former studies reported great ANA predisposition in CHC and these ANAs may be related to worse prognosis including hepatocellular carcinoma (HCC). We aimed to evaluate the association between ANA incidence and CHC-related HCC development and to evaluate these molecules effect on HCC severity including tumor size and advanced stages. Results revealed that ANA seropositivity was associated with disease severity. HCC patients (54%, OR = 9.7) were associated with ANA positivity more than liver cirrhosis (24.5%) and fibrosis (10.8%). ANA positivity was significantly high in patients with severe tumor features including macrovascular invasion (61.9%; OR = 8.1), large size (68.2%; OR = 2.4), Child C (83.3%; OR = 8.1), BCLC end stage (83.3%; OR = 8.6) and advanced CLIP stage (80.9%; OR = 7.9). ANA positivity were significantly (p < 0.05) correlated with some estimated liver fibrosis related biomarkers including EMA (r = 0.206), fibronectin (r = 0.273), cytokeratin-1 (r = 0.365) and collagen III (r = 0.324). In conclusion, our observation of increased ANA+ serum samples among CHC-related HCC might suggest the oncogenic role of ANA in such patients. Also, clinicians need to appreciate value of ANA testing among HCC patients as these molecules were associated with tumor severity and worse outcomes.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical expression of epidermal growth factor receptor: prognostic value in HER2 positive breast cancer.

Journal of immunoassay & immunochemistry Pub Date : 2025-03-09 DOI: 10.1080/15321819.2025.2475291

Yoldez Houcine, Hend Ben Salem, Sirine El Fekih, Amal Maaoui, Maha Driss

{"title":"Immunohistochemical expression of epidermal growth factor receptor: prognostic value in HER2 positive breast cancer.","authors":"Yoldez Houcine, Hend Ben Salem, Sirine El Fekih, Amal Maaoui, Maha Driss","doi":"10.1080/15321819.2025.2475291","DOIUrl":"https://doi.org/10.1080/15321819.2025.2475291","url":null,"abstract":"Introduction: Epidermal Growth Factor Receptor (EGFR) expression is not well-studied in Human Epidermal Growth Factor Receptor 2 (HER2) positive breast cancer. We aim to study the prevalence of EGFR immunohistochemical expression in HER2-positive breast carcinomas and to correlate this expression with different anatomo-clinical parameters.Methods: It was a retrospective study involving cases of HER2-positive breast carcinoma collected at the Immuno-Histo-Cytology Department of Salah Azaïez Institute of Tunis between 2018 and 2020. An immunohistochemical study using the anti-human EGFR monoclonal antibody was performed. Cases with an overall score ≥1+ were considered positive.Results: Fifty patients were included. EGFR expression in HER2-positive breast carcinomas was more likely to occur in patients under the age of 50 (p = 0.063). It was significantly associated with the absence of lymphovascular invasion (p = 0.047). In multivariate analysis, young age, absence of lympho-vascular invasion, and high Ki67 proliferation index (>60%) were independently associated with positive EGFR expression (p = 0.047, p = 0.040, and p = 0.050, respectively).Conclusion: Through this first Tunisian study, our data revealed that the immunohistochemical expression of EGFR is associated with young age, absence of lymphovascular invasion, and a high mitotic index (Ki67), which may suggest a potential predictive value for chemotherapy response.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Ki67 in pathological prognostic staging of breast cancer: a tertiary care center study. Ki67在乳腺癌病理预后分期中的评价：一项三级保健中心研究。

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2025-01-15 DOI: 10.1080/15321819.2025.2451211

Rashmi Wankhade, Arvind Bhake, Nandkishor Bankar, Yugeshwari Tiwade

{"title":"Evaluation of Ki67 in pathological prognostic staging of breast cancer: a tertiary care center study.","authors":"Rashmi Wankhade, Arvind Bhake, Nandkishor Bankar, Yugeshwari Tiwade","doi":"10.1080/15321819.2025.2451211","DOIUrl":"10.1080/15321819.2025.2451211","url":null,"abstract":"Background: The rising global burden of breast cancer demands early detection and effective treatment, with a focus on prognostic and predictive markers. The eighth edition of the American Joint Committee on Cancer staging manual introduced a new prognostic staging system to increase the predictive power of the existing anatomical staging system of breast cancer. The current study aimed to establish the correlation between Ki67 expression with molecular subtypes and with the pathological prognostic stage of invasive ductal carcinoma.Materials and methods: A total of 40 patients were included in the study with samples from 32 modified radical mastectomies and 8 biopsies. Hematoxylin and Eosin staining, histopathological analysis and Ki67 immunostaining were conducted. Descriptive and inferential statistical analyses were performed.Results: Bloom Richardson Grade II was the predominant histological grade. In Grade II cases, 15 of 24 had a Ki67 labeling index of 26-45%, while 6 exceeded 45% (p = 0.001). Pathological prognostic staging reclassified 27 cases, with 24 (75%) downstaged, 3 (9.38%) upstaged, and 5 (15.63%) retaining their clinical stage.Conclusions: Ki67 immunohistochemistry is an effective tool for assessing proliferative activity of invasive ductal carcinoma, aiding in pathological prognostic stage stratification and offering insights into tumor biology.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"186-200"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparison between different chemical fractionation methods for immunoglobulin preparation. 制备免疫球蛋白不同化学分馏方法的比较。

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2025-01-11 DOI: 10.1080/15321819.2025.2450664

Abbasali Salarifar, Pooria Safarzadeh Kozani, Mohammad Javad Rasaee

{"title":"A comparison between different chemical fractionation methods for immunoglobulin preparation.","authors":"Abbasali Salarifar, Pooria Safarzadeh Kozani, Mohammad Javad Rasaee","doi":"10.1080/15321819.2025.2450664","DOIUrl":"10.1080/15321819.2025.2450664","url":null,"abstract":"Background: Application of antibodies in therapeutics and diagnostics are growing Continually. Herein, we aimed to find the most qualified immunoglobulin (Ig) chemical preparation method.Methods: A rabbit was immunized against recombinant SARS-CoV-2 nucleocapsid (NP) and reactive polyclonal antibodies were prepared using the ammonium sulfate (AS), caprylic acid (CA), polyethylene glycol (PEG), and caprylic acid/ammonium sulfate (CA/AS) methods. Different antibody solutions were analyzed by SDS-PAGE and subsequently quantified by ImageJ software for further analysis in terms of Ig purity, Ig recovery, and albumin impurity. Ultimately, the prepared antibodies were assessed via Western blotting and ELISA to evaluate their ability to bind NP.Results: Prepared Ig solutions via the CA/AS method had the highest Ig purity (followed by CA, PEG, and AS) and lowest albumin impurity (followed by CA, AS, and PEG). The PEG method had the highest recovery followed by AS, CA, and CA/AS methods. Moreover, antibodies prepared via different methods demonstrated comparable binding capacities to NP in ELISA and Western blotting.Conclusions: CA/AS, closely followed by CA, proved to be the most qualified method for the preparation of Ig yielding the highest Ig purity while the PEG method resulted in the highest Ig recovery rate.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"169-185"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimate of circRNAs and microRNAs synergies on clinical advance of psoriasis.

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2024-12-30 DOI: 10.1080/15321819.2024.2447726

Amany A Saleh, Safaa I Tayel, Wafaa A Shehata, Eman A El-Masry, Huda I Abd-Elhafiz, Mohammed G Elhelbawy, Sherin S El-Naidany

{"title":"Estimate of circRNAs and microRNAs synergies on clinical advance of psoriasis.","authors":"Amany A Saleh, Safaa I Tayel, Wafaa A Shehata, Eman A El-Masry, Huda I Abd-Elhafiz, Mohammed G Elhelbawy, Sherin S El-Naidany","doi":"10.1080/15321819.2024.2447726","DOIUrl":"https://doi.org/10.1080/15321819.2024.2447726","url":null,"abstract":"Background: Noncoding RNAs have recently proven their contributing role in the pathogenesis of numerous diseases. The etiology of psoriasis is still not well defined, and genetic and environmental factors are blamed. We meant to explore the association of circRNAs/microRNAs in psoriasis and inspect their correlation with the clinical features of the disease.Design and methods: This study included 166 subjects: 83 cases with plaque psoriasis and 83 age- and sex-matched healthy persons as the control group. The expression levels of circRNA000102, circRNA0045272, circRNA0084764, and microRNAs -93,181, 16a, and 21 were estimated by quantitative real-time PCR.Results: CircRNA0045272 was ten-fold up-regulated, whereas circRNA0084764 and circRNA000102 were about two-fold downregulated in patients vs. control. MicroRNAs -93 and 181 were significantly downregulated in patients vs. control, whereas microRNA -16a and 21 were up-regulated in patients vs. control. CircRNA0045272 was positively correlated with microRNA 181, and circRNA0084764 was positively correlated with microRNA 93 and microRNA 181. CircRNA0045272 had the highest sensitivity (98.8%) and specificity (93.98%), and microRNA-16a had sensitivity (98.80%) with specificity (93.98%).Conclusion: We assume that circRNAs can predict psoriasis occurrence and progression by sponging a set of microRNAs and can modulate several genes that disturb immune cell balance and inflammatory elements in psoriasis.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"46 2","pages":"147-168"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0