Journal of immunoassay & immunochemistry最新文献_第2页

Development and validation of a cell-based methodology to evaluate filgrastim relative affinity to granulocyte colony-stimulating factor receptor. 基于细胞的非格昔汀对粒细胞集落刺激因子受体的相对亲和力评价方法的发展和验证。

Journal of immunoassay & immunochemistry Pub Date : 2026-01-01 Epub Date: 2025-12-19 DOI: 10.1080/15321819.2025.2594998

Víctor R Campos-García, Lilia Tierrablanca-Sánchez, Nancy D Ramírez-Ibáñez, Néstor O Pérez, José González-Valdez

{"title":"Development and validation of a cell-based methodology to evaluate filgrastim relative affinity to granulocyte colony-stimulating factor receptor.","authors":"Víctor R Campos-García, Lilia Tierrablanca-Sánchez, Nancy D Ramírez-Ibáñez, Néstor O Pérez, José González-Valdez","doi":"10.1080/15321819.2025.2594998","DOIUrl":"10.1080/15321819.2025.2594998","url":null,"abstract":"Filgrastim, a therapeutic protein for the treatment of neutropenia, exerts its biological activity by interacting with the granulocyte colony-stimulating factor receptor. However, the affinity of filgrastim to its receptor is usually overlooked during characterization and comparability tests, since its biological activity is assessed only by in vitro proliferation assays. In this work, we propose the use of a cell-based colorimetric method to determine the relative affinity of filgrastim to its receptor expressed in murine myeloid leukemia cells. After performing a validation exercise, the method proved to be accurate, specific, and linear within an affinity interval of 75 to 130%; precise with a confidence interval of 93.6 to 114.2% and a coefficient of variation of 12.6%. The validation exercise proved that the proposed cell-based method is a viable alternative to evaluate the biological activity of filgrastim via the affinity for its receptor and it is suitable to be included as part of its biological characterization exercises as well as in comparability exercises for products coming from distinct manufacturing processes.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"60-76"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145794049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pivotal role of interleukin 27 serum level and missense variant (rs181206) in multiple sclerosis. 白介素27血清水平和错义变异（rs181206）在多发性硬化中的关键作用。

Journal of immunoassay & immunochemistry Pub Date : 2026-01-01 Epub Date: 2026-02-04 DOI: 10.1080/15321819.2026.2623279

Amany E Elashkar, Marwa M Khalil, Mona S Elkholy, Eman Samy, Sanaa S Hamam, Amira Samy Elmaghraby

{"title":"Pivotal role of interleukin 27 serum level and missense variant (rs181206) in multiple sclerosis.","authors":"Amany E Elashkar, Marwa M Khalil, Mona S Elkholy, Eman Samy, Sanaa S Hamam, Amira Samy Elmaghraby","doi":"10.1080/15321819.2026.2623279","DOIUrl":"10.1080/15321819.2026.2623279","url":null,"abstract":"The incidence of multiple sclerosis (MS) has increased in recent years. Its pathogenesis involves the interaction between various elements, with interleukin 27 (IL-27) playing a key role in autoimmunity. The presence of the IL-27 receptor on astrocytes emphasizes its involvement in the disease's progression.Purpose: The study aims to investigate possible associations between IL27 rs181206, serum level of IL27, and the development of MS.Methods: The study comprised 70 MS patients and 70 seemingly healthy controls. They were genotyped for IL27 rs181206 using the Taqman allelic discrimination approach, and their serum IL27 levels were estimated using ELISA.Results: The frequency of TT genotype, T allele, and IL27 serum level were significantly higher among MS patients compared to controls. There was no significant difference between IL27 serum levels among different genotypes in both MS patients and controls; however, individuals with TT genotype showed higher levels of IL27 than those with CC genotype.Conclusion: TT genotype and T allele can increase the risk of developing MS. On the other hand, carrying the C allele may be associated with a lower risk of MS development. Understanding IL27 genetics and epistatic interactions can help clarify IL27's role in MS pathogenesis and utilize it as a therapeutic target.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"113-128"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146119150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Burcu Kök Kendirlioğlu, MD, PhD -Early Career Prize Winner 2024. Burcu Kök Kendirlioğlu，医学博士，博士- 2024年早期职业奖得主。

Journal of immunoassay & immunochemistry Pub Date : 2025-04-20 DOI: 10.1080/15321819.2025.2496044

引用次数: 0

Evaluation of Ki67 in pathological prognostic staging of breast cancer: a tertiary care center study. Ki67在乳腺癌病理预后分期中的评价：一项三级保健中心研究。

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2025-01-15 DOI: 10.1080/15321819.2025.2451211

Rashmi Wankhade, Arvind Bhake, Nandkishor Bankar, Yugeshwari Tiwade

{"title":"Evaluation of Ki67 in pathological prognostic staging of breast cancer: a tertiary care center study.","authors":"Rashmi Wankhade, Arvind Bhake, Nandkishor Bankar, Yugeshwari Tiwade","doi":"10.1080/15321819.2025.2451211","DOIUrl":"10.1080/15321819.2025.2451211","url":null,"abstract":"Background: The rising global burden of breast cancer demands early detection and effective treatment, with a focus on prognostic and predictive markers. The eighth edition of the American Joint Committee on Cancer staging manual introduced a new prognostic staging system to increase the predictive power of the existing anatomical staging system of breast cancer. The current study aimed to establish the correlation between Ki67 expression with molecular subtypes and with the pathological prognostic stage of invasive ductal carcinoma.Materials and methods: A total of 40 patients were included in the study with samples from 32 modified radical mastectomies and 8 biopsies. Hematoxylin and Eosin staining, histopathological analysis and Ki67 immunostaining were conducted. Descriptive and inferential statistical analyses were performed.Results: Bloom Richardson Grade II was the predominant histological grade. In Grade II cases, 15 of 24 had a Ki67 labeling index of 26-45%, while 6 exceeded 45% (p = 0.001). Pathological prognostic staging reclassified 27 cases, with 24 (75%) downstaged, 3 (9.38%) upstaged, and 5 (15.63%) retaining their clinical stage.Conclusions: Ki67 immunohistochemistry is an effective tool for assessing proliferative activity of invasive ductal carcinoma, aiding in pathological prognostic stage stratification and offering insights into tumor biology.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"186-200"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparison between different chemical fractionation methods for immunoglobulin preparation. 制备免疫球蛋白不同化学分馏方法的比较。

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2025-01-11 DOI: 10.1080/15321819.2025.2450664

Abbasali Salarifar, Pooria Safarzadeh Kozani, Mohammad Javad Rasaee

{"title":"A comparison between different chemical fractionation methods for immunoglobulin preparation.","authors":"Abbasali Salarifar, Pooria Safarzadeh Kozani, Mohammad Javad Rasaee","doi":"10.1080/15321819.2025.2450664","DOIUrl":"10.1080/15321819.2025.2450664","url":null,"abstract":"Background: Application of antibodies in therapeutics and diagnostics are growing Continually. Herein, we aimed to find the most qualified immunoglobulin (Ig) chemical preparation method.Methods: A rabbit was immunized against recombinant SARS-CoV-2 nucleocapsid (NP) and reactive polyclonal antibodies were prepared using the ammonium sulfate (AS), caprylic acid (CA), polyethylene glycol (PEG), and caprylic acid/ammonium sulfate (CA/AS) methods. Different antibody solutions were analyzed by SDS-PAGE and subsequently quantified by ImageJ software for further analysis in terms of Ig purity, Ig recovery, and albumin impurity. Ultimately, the prepared antibodies were assessed via Western blotting and ELISA to evaluate their ability to bind NP.Results: Prepared Ig solutions via the CA/AS method had the highest Ig purity (followed by CA, PEG, and AS) and lowest albumin impurity (followed by CA, AS, and PEG). The PEG method had the highest recovery followed by AS, CA, and CA/AS methods. Moreover, antibodies prepared via different methods demonstrated comparable binding capacities to NP in ELISA and Western blotting.Conclusions: CA/AS, closely followed by CA, proved to be the most qualified method for the preparation of Ig yielding the highest Ig purity while the PEG method resulted in the highest Ig recovery rate.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"169-185"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimate of circRNAs and microRNAs synergies on clinical advance of psoriasis. circRNAs和microRNAs在银屑病临床进展中的协同作用评估。

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2024-12-30 DOI: 10.1080/15321819.2024.2447726

Amany A Saleh, Safaa I Tayel, Wafaa A Shehata, Eman A El-Masry, Huda I Abd-Elhafiz, Mohammed G Elhelbawy, Sherin S El-Naidany

{"title":"Estimate of circRNAs and microRNAs synergies on clinical advance of psoriasis.","authors":"Amany A Saleh, Safaa I Tayel, Wafaa A Shehata, Eman A El-Masry, Huda I Abd-Elhafiz, Mohammed G Elhelbawy, Sherin S El-Naidany","doi":"10.1080/15321819.2024.2447726","DOIUrl":"10.1080/15321819.2024.2447726","url":null,"abstract":"Background: Noncoding RNAs have recently proven their contributing role in the pathogenesis of numerous diseases. The etiology of psoriasis is still not well defined, and genetic and environmental factors are blamed. We meant to explore the association of circRNAs/microRNAs in psoriasis and inspect their correlation with the clinical features of the disease.Design and methods: This study included 166 subjects: 83 cases with plaque psoriasis and 83 age- and sex-matched healthy persons as the control group. The expression levels of circRNA000102, circRNA0045272, circRNA0084764, and microRNAs -93,181, 16a, and 21 were estimated by quantitative real-time PCR.Results: CircRNA0045272 was ten-fold up-regulated, whereas circRNA0084764 and circRNA000102 were about two-fold downregulated in patients vs. control. MicroRNAs -93 and 181 were significantly downregulated in patients vs. control, whereas microRNA -16a and 21 were up-regulated in patients vs. control. CircRNA0045272 was positively correlated with microRNA 181, and circRNA0084764 was positively correlated with microRNA 93 and microRNA 181. CircRNA0045272 had the highest sensitivity (98.8%) and specificity (93.98%), and microRNA-16a had sensitivity (98.80%) with specificity (93.98%).Conclusion: We assume that circRNAs can predict psoriasis occurrence and progression by sponging a set of microRNAs and can modulate several genes that disturb immune cell balance and inflammatory elements in psoriasis.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"46 2","pages":"147-168"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral human papillomavirus infection and genotyping in a cohort of people living with HIV. 一组HIV感染者的口腔人乳头瘤病毒感染和基因分型

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2024-12-23 DOI: 10.1080/15321819.2024.2441778

O F Adebayo, F J Owotade, O A Folarin, O A Oninla, E O Oyetola

{"title":"Oral human papillomavirus infection and genotyping in a cohort of people living with HIV.","authors":"O F Adebayo, F J Owotade, O A Folarin, O A Oninla, E O Oyetola","doi":"10.1080/15321819.2024.2441778","DOIUrl":"10.1080/15321819.2024.2441778","url":null,"abstract":"This study is aimed at determining the prevalence of oral HPV infection and the risk indicators for oral HPV carriage in people living with HIV. Data on socio-demographics, sexual behavioral practices, and lifestyle practices of the participants were collected from 66 people living with HIV. The HIV parameters of each study participant were obtained from clinical records. Oral rinses obtained from each participant were subjected to HPV ELISA antigen test for screening and extracted DNA was subjected to nested PCR and whole-genome sequencing for genotyping. Approximately 36% (10 of 28) HIV-positive individuals had oral HPV carriage with one person carrying oncogenic type, HPV16. In addition, 80% (8 of 10) of those with HPV positivity by PCR are females, but with no statistically significant association. The CD4 count showed no significant association with oral HPV carriage in HIV positive individuals; however, age at first sex is a determinant of oral HPV infection in people living with HIV with positive association observed on both bivariate analysis and logistic regression (AOR: 150.49, 95% CI: 1.40-16,155.47, p = 0.036).","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"129-146"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A family cluster of persistent Pandoraea vervacti infection in cystic fibrosis. 囊性纤维化患者持续性vervaci潘多拉菌感染的家族聚集性。

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2025-02-11 DOI: 10.1080/15321819.2025.2462807

Camilla Bitossi, Matteo Fracella, Agnese Viscido, Fabiana Diaco, Claudia Pecoraro, Patrizia Troiani, Giuseppe Cimino, Gabriella d'Ettorre, Maria Trancassini, Alessandra Pierangeli, Guido Antonelli, Carolina Scagnolari

{"title":"A family cluster of persistent Pandoraea vervacti infection in cystic fibrosis.","authors":"Camilla Bitossi, Matteo Fracella, Agnese Viscido, Fabiana Diaco, Claudia Pecoraro, Patrizia Troiani, Giuseppe Cimino, Gabriella d'Ettorre, Maria Trancassini, Alessandra Pierangeli, Guido Antonelli, Carolina Scagnolari","doi":"10.1080/15321819.2025.2462807","DOIUrl":"10.1080/15321819.2025.2462807","url":null,"abstract":"Bacterial colonization of the cystic fibrosis (CF) airways is polymicrobial and several emerging microorganisms with a potential pathogenic role may be present. We report a case of three siblings with CF colonized by Pandoraea over a period of 10 years. Isolates identified as various non-fermentative Gram-negative bacilli from sputum cultures were successfully re-identified as Pandoraea vervacti (P. vervacti) by a combination of matrix-assisted laser desorption ionization - time of flight mass spectrometry (MALDI-TOF MS) and 16S rRNA and gyrB sequencing. Furthermore, the transcript expression of type I and type III interferon (IFN) genes was examined in the cells of respiratory samples from these patients and compared with a Pandoraea-negative group of CF individuals. Increased respiratory levels of IFNβ, IFNε and IL28R1 mRNA were found in the three siblings. Our results demonstrate that P. vervacti can chronically colonize CF patients and alter IFN response, likely contributing to immunopathogenesis and disease progression.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"201-206"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adults with low opsonic natural antibody levels against Streptococcus pneumoniae show enhanced response to PPSV23 vaccination. 肺炎链球菌天然抗体水平低的成年人对接种 PPSV23 疫苗的反应增强。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-02 Epub Date: 2024-11-19 DOI: 10.1080/15321819.2024.2430344

Sravani Dharmavaram, Geetha Nagaraj, Sundaresan Natesan, Manjula Subbanna, Ravikumar Kadahalli Lingegowda

{"title":"Adults with low opsonic natural antibody levels against Streptococcus pneumoniae show enhanced response to PPSV23 vaccination.","authors":"Sravani Dharmavaram, Geetha Nagaraj, Sundaresan Natesan, Manjula Subbanna, Ravikumar Kadahalli Lingegowda","doi":"10.1080/15321819.2024.2430344","DOIUrl":"10.1080/15321819.2024.2430344","url":null,"abstract":"Background: Pneumococcal diseases pose a significant public health concern globally, particularly among young children and the elderly. Vaccination plays a crucial role in their prevention. This study evaluated the functional immune responses to Pneumococcal polysaccharide vaccine serotypes in healthy Indian adults before and after administering a single dose of PPSV23 immunization.Methods: A total of 125 healthy participants aged 18-65 received the PPSV23 vaccine, and their pre- and post-immunization sera were analyzed by MOPA. Opsonic Index, Geometric mean OPA titers and fold increase for each serotype was calculated.Results: The highest baseline OPA GMTs were observed for serotypes 33F,17F,9N,20, and 6B. The lowest OPA GMTs were noted against types 1 and 12F. OPA GMTs post-vaccination increased significantly for all serotypes, with geometric mean fold rise (GMFR) ranging from 4.3 to 267.5. Participants with low pre-immunization OPA titers (<8 & <64) showed significant increases in OI fold raise across all tested serotypes post-vaccination. This robust immune response was consistent across serotypes, indicating highly effective seroconversion in individuals with low baseline antibody levels.Conclusion: The PPSV23 vaccine elicits a strong immunogenic response in individuals with low pre-immunization OPA titers, achieving substantial increases in opsonic index fold raise across various serotypes.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"29-48"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging therapies and innovations in vitiligo management: a comprehensive review. 白癜风治疗中的新兴疗法和创新：全面回顾。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-02 Epub Date: 2024-10-06 DOI: 10.1080/15321819.2024.2412528

Manjusha Bhange, Sachin Kothawade, Darshan Telange, Vijaya Padwal

{"title":"Emerging therapies and innovations in vitiligo management: a comprehensive review.","authors":"Manjusha Bhange, Sachin Kothawade, Darshan Telange, Vijaya Padwal","doi":"10.1080/15321819.2024.2412528","DOIUrl":"10.1080/15321819.2024.2412528","url":null,"abstract":"Vitiligo is a common skin disorder where melanocytes, the cells that produce skin pigment, are destroyed by the immune system, leading to white patches on the skin and mucous membranes. This condition affects 0.4% to 2.0% of the global population, with a higher prevalence in females and often beginning in childhood. In India, about 1% of the population is affected, particularly in northern regions, with a higher incidence in females and links to other autoimmune diseases. This review examines recent progress in understanding vitiligo and its treatment. It focuses on the genetic, autoimmune, and environmental factors involved in the disease and highlights new therapies, such as targeted molecular treatments and advanced repigmentation methods. Current research shows that oxidative stress and genetic predispositions contribute to the autoimmune destruction of melanocytes. Novel drug delivery systems, including liposomes, nanoemulsions, and nanostructured lipid carriers, have improved treatment effectiveness. Clinical trials are exploring new treatments like Ruxolitinib cream and melanocyte transplantation, while teledermatology is becoming useful for managing patients. Vitiligo also poses a significant economic burden due to its impact on patients' quality of life. Continued research is essential to develop better, more accessible treatments and reduce the economic impact of vitiligo.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-28"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0