Journal of immunoassay & immunochemistry最新文献_第2页

Oral human papillomavirus infection and genotyping in a cohort of people living with HIV. 一组HIV感染者的口腔人乳头瘤病毒感染和基因分型

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2024-12-23 DOI: 10.1080/15321819.2024.2441778

O F Adebayo, F J Owotade, O A Folarin, O A Oninla, E O Oyetola

{"title":"Oral human papillomavirus infection and genotyping in a cohort of people living with HIV.","authors":"O F Adebayo, F J Owotade, O A Folarin, O A Oninla, E O Oyetola","doi":"10.1080/15321819.2024.2441778","DOIUrl":"10.1080/15321819.2024.2441778","url":null,"abstract":"This study is aimed at determining the prevalence of oral HPV infection and the risk indicators for oral HPV carriage in people living with HIV. Data on socio-demographics, sexual behavioral practices, and lifestyle practices of the participants were collected from 66 people living with HIV. The HIV parameters of each study participant were obtained from clinical records. Oral rinses obtained from each participant were subjected to HPV ELISA antigen test for screening and extracted DNA was subjected to nested PCR and whole-genome sequencing for genotyping. Approximately 36% (10 of 28) HIV-positive individuals had oral HPV carriage with one person carrying oncogenic type, HPV16. In addition, 80% (8 of 10) of those with HPV positivity by PCR are females, but with no statistically significant association. The CD4 count showed no significant association with oral HPV carriage in HIV positive individuals; however, age at first sex is a determinant of oral HPV infection in people living with HIV with positive association observed on both bivariate analysis and logistic regression (AOR: 150.49, 95% CI: 1.40-16,155.47, p = 0.036).","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"129-146"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A family cluster of persistent Pandoraea vervacti infection in cystic fibrosis.

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2025-02-11 DOI: 10.1080/15321819.2025.2462807

Camilla Bitossi, Matteo Fracella, Agnese Viscido, Fabiana Diaco, Claudia Pecoraro, Patrizia Troiani, Giuseppe Cimino, Gabriella d'Ettorre, Maria Trancassini, Alessandra Pierangeli, Guido Antonelli, Carolina Scagnolari

{"title":"A family cluster of persistent Pandoraea vervacti infection in cystic fibrosis.","authors":"Camilla Bitossi, Matteo Fracella, Agnese Viscido, Fabiana Diaco, Claudia Pecoraro, Patrizia Troiani, Giuseppe Cimino, Gabriella d'Ettorre, Maria Trancassini, Alessandra Pierangeli, Guido Antonelli, Carolina Scagnolari","doi":"10.1080/15321819.2025.2462807","DOIUrl":"10.1080/15321819.2025.2462807","url":null,"abstract":"Bacterial colonization of the cystic fibrosis (CF) airways is polymicrobial and several emerging microorganisms with a potential pathogenic role may be present. We report a case of three siblings with CF colonized by Pandoraea over a period of 10 years. Isolates identified as various non-fermentative Gram-negative bacilli from sputum cultures were successfully re-identified as Pandoraea vervacti (P. vervacti) by a combination of matrix-assisted laser desorption ionization - time of flight mass spectrometry (MALDI-TOF MS) and 16S rRNA and gyrB sequencing. Furthermore, the transcript expression of type I and type III interferon (IFN) genes was examined in the cells of respiratory samples from these patients and compared with a Pandoraea-negative group of CF individuals. Increased respiratory levels of IFNβ, IFNε and IL28R1 mRNA were found in the three siblings. Our results demonstrate that P. vervacti can chronically colonize CF patients and alter IFN response, likely contributing to immunopathogenesis and disease progression.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"201-206"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lymphangioinvasion detection using the monoclonal antibody D2-40 (Podoplanin)as a clinical predictor of axillary lymph node metastasis in breast cancer patients.

Journal of immunoassay & immunochemistry Pub Date : 2025-02-23 DOI: 10.1080/15321819.2025.2470434

Teresa CristinaFerreira Gutman, Angela de Salles Rezende, Dyego Mondego Moraes, Consuelo Lozoya Lopez, Licínio Esmeraldo da Silva, Rafaela Elvira Rozza-de-Menezes, João Paulo Lima Daher, André Vallejo da Silva, Fabiana Resende Rodrigues, Vânia Gloria Silami Lopes

{"title":"Lymphangioinvasion detection using the monoclonal antibody D2-40 (Podoplanin)as a clinical predictor of axillary lymph node metastasis in breast cancer patients.","authors":"Teresa CristinaFerreira Gutman, Angela de Salles Rezende, Dyego Mondego Moraes, Consuelo Lozoya Lopez, Licínio Esmeraldo da Silva, Rafaela Elvira Rozza-de-Menezes, João Paulo Lima Daher, André Vallejo da Silva, Fabiana Resende Rodrigues, Vânia Gloria Silami Lopes","doi":"10.1080/15321819.2025.2470434","DOIUrl":"https://doi.org/10.1080/15321819.2025.2470434","url":null,"abstract":"Breast is a major global health issue and the most common cancer in women. Identifying vascular invasion is challenging due to the need to distinguish true invasion from artifacts. This study explored lymphatic embolism in invasive breast carcinoma using the monoclonal antibody D2-40 as a prognostic indicator. A total of 100 patients with invasive breast carcinoma from 2009 to 2011 were included in the study. Tissue microarray technique (TMA) was used on patient tissue, constructing three paraffin blocks from each participant's histological data. Immunohistochemistry with D2-40 and CD34 antibodies was performed to identify lymphatic and blood emboli, respectively, and results were compared with previous findings. A prior report using hematoxylin-eosin staining found fewer patients with lymphatic emboli (34) compared to our study (56) using D2-40. Lymphatic emboli correlated with axillary metastases, with an odds ratio (OR) of 3.50, a 95% confidence interval (CI) of 1.92-5.08, and a p-value of 0.001, whereas hematoxylin-eosin alone showed OR = 1.42, 95% CI = 0.40-3.47, and p-value = 0.23. TMA with D2-40 staining detected more lymphatic emboli than hematoxylin-eosin staining alone. Higher embolic expression rates are linked to increased tumor aggressiveness, worse prognosis and shorter overall survival.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implication of TRPM8, CD47, and CDK4 expressions in hepatocellular carcinoma progression.

Journal of immunoassay & immunochemistry Pub Date : 2025-02-16 DOI: 10.1080/15321819.2025.2464718

Aiat Shaban Hemida, Mona Saeed Tantawy

{"title":"Implication of TRPM8, CD47, and CDK4 expressions in hepatocellular carcinoma progression.","authors":"Aiat Shaban Hemida, Mona Saeed Tantawy","doi":"10.1080/15321819.2025.2464718","DOIUrl":"https://doi.org/10.1080/15321819.2025.2464718","url":null,"abstract":"Participation of TRPM8 in hepatocellular carcinoma (HCC) development was not precisely declared. CD47 mediates immune escape and macrophage phagocytosis of tumors. CDK4 mediates oncogenesis. Synergistic implications of TRPM8, CD47, and CDK4 in HCC were not declared. This research aims to demonstrate the expressions of TRPM8, CD47and CDK4 in HCC and to declare correlations and significance. Paraffin blocks from 101 hCC and 82 adjacent non-tumorous liver were immunostained using TRPM8, CD47 and CDK4 antibodies. HCC showed highly significant increased TRPM8, CD47, and CDK4 expressions than control liver tissue (p < 0.001) for all. TRPM8, CD47, and CDK4 were significantly associated with poor prognostic criteria as high tumor grade, advanced stage, microvascular invasion, and necrosis. There was a significant association between cirrhotic and non-cirrhotic adjacent liver regarding positive TRPM8 (p < 0.02) and high CDK4 expressions (p < 0.045). There were significant direct relationships between each immunohistochemical antibody and the other two. Prolonged overall survival was significantly associated with low CDK4 (p = 0.019). In conclusion, TRPM8, CD47, and CDK4 may regulate synergistic functions in HCC oncogenesis and accomplish unfavorable prognostic significance. TRPM8 and CDK4 might share in development of HCC from cirrhosis. TRPM8, CD47, and CDK4 could be therapeutic targets in HCC.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-17"},"PeriodicalIF":0.0,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adults with low opsonic natural antibody levels against Streptococcus pneumoniae show enhanced response to PPSV23 vaccination. 肺炎链球菌天然抗体水平低的成年人对接种 PPSV23 疫苗的反应增强。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-02 Epub Date: 2024-11-19 DOI: 10.1080/15321819.2024.2430344

Sravani Dharmavaram, Geetha Nagaraj, Sundaresan Natesan, Manjula Subbanna, Ravikumar Kadahalli Lingegowda

{"title":"Adults with low opsonic natural antibody levels against Streptococcus pneumoniae show enhanced response to PPSV23 vaccination.","authors":"Sravani Dharmavaram, Geetha Nagaraj, Sundaresan Natesan, Manjula Subbanna, Ravikumar Kadahalli Lingegowda","doi":"10.1080/15321819.2024.2430344","DOIUrl":"10.1080/15321819.2024.2430344","url":null,"abstract":"Background: Pneumococcal diseases pose a significant public health concern globally, particularly among young children and the elderly. Vaccination plays a crucial role in their prevention. This study evaluated the functional immune responses to Pneumococcal polysaccharide vaccine serotypes in healthy Indian adults before and after administering a single dose of PPSV23 immunization.Methods: A total of 125 healthy participants aged 18-65 received the PPSV23 vaccine, and their pre- and post-immunization sera were analyzed by MOPA. Opsonic Index, Geometric mean OPA titers and fold increase for each serotype was calculated.Results: The highest baseline OPA GMTs were observed for serotypes 33F,17F,9N,20, and 6B. The lowest OPA GMTs were noted against types 1 and 12F. OPA GMTs post-vaccination increased significantly for all serotypes, with geometric mean fold rise (GMFR) ranging from 4.3 to 267.5. Participants with low pre-immunization OPA titers (<8 & <64) showed significant increases in OI fold raise across all tested serotypes post-vaccination. This robust immune response was consistent across serotypes, indicating highly effective seroconversion in individuals with low baseline antibody levels.Conclusion: The PPSV23 vaccine elicits a strong immunogenic response in individuals with low pre-immunization OPA titers, achieving substantial increases in opsonic index fold raise across various serotypes.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"29-48"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging therapies and innovations in vitiligo management: a comprehensive review. 白癜风治疗中的新兴疗法和创新：全面回顾。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-02 Epub Date: 2024-10-06 DOI: 10.1080/15321819.2024.2412528

Manjusha Bhange, Sachin Kothawade, Darshan Telange, Vijaya Padwal

{"title":"Emerging therapies and innovations in vitiligo management: a comprehensive review.","authors":"Manjusha Bhange, Sachin Kothawade, Darshan Telange, Vijaya Padwal","doi":"10.1080/15321819.2024.2412528","DOIUrl":"10.1080/15321819.2024.2412528","url":null,"abstract":"Vitiligo is a common skin disorder where melanocytes, the cells that produce skin pigment, are destroyed by the immune system, leading to white patches on the skin and mucous membranes. This condition affects 0.4% to 2.0% of the global population, with a higher prevalence in females and often beginning in childhood. In India, about 1% of the population is affected, particularly in northern regions, with a higher incidence in females and links to other autoimmune diseases. This review examines recent progress in understanding vitiligo and its treatment. It focuses on the genetic, autoimmune, and environmental factors involved in the disease and highlights new therapies, such as targeted molecular treatments and advanced repigmentation methods. Current research shows that oxidative stress and genetic predispositions contribute to the autoimmune destruction of melanocytes. Novel drug delivery systems, including liposomes, nanoemulsions, and nanostructured lipid carriers, have improved treatment effectiveness. Clinical trials are exploring new treatments like Ruxolitinib cream and melanocyte transplantation, while teledermatology is becoming useful for managing patients. Vitiligo also poses a significant economic burden due to its impact on patients' quality of life. Continued research is essential to develop better, more accessible treatments and reduce the economic impact of vitiligo.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"1-28"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analytical interference of Burosumab therapy on intact fibroblast growth factor 23 (iFGF23) measurements using an immunoassay: preliminary evaluation. 使用免疫测定法分析布苏单抗疗法对完整成纤维细胞生长因子 23 (iFGF23) 测量的干扰：初步评估。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-02 Epub Date: 2024-11-04 DOI: 10.1080/15321819.2024.2422098

Vincenzo Brescia, Roberto Lovero, Antonietta Fontana, Francesca Di Serio, Marica Colella, Vincenza Carbone, Marika Giliberti, Maria Grazia Perrone, Antonio Scilimati, Raffaele Palmirotta

{"title":"Analytical interference of Burosumab therapy on intact fibroblast growth factor 23 (iFGF23) measurements using an immunoassay: preliminary evaluation.","authors":"Vincenzo Brescia, Roberto Lovero, Antonietta Fontana, Francesca Di Serio, Marica Colella, Vincenza Carbone, Marika Giliberti, Maria Grazia Perrone, Antonio Scilimati, Raffaele Palmirotta","doi":"10.1080/15321819.2024.2422098","DOIUrl":"10.1080/15321819.2024.2422098","url":null,"abstract":"Our study evaluated the possible interference of Burosumab (human recombinant monoclonal antibody directed against N-terminal domain of FGF23) on the immunoassay of intact FGF23 (iFGF23) with the Liaison XL. The analytical method uses three different antibodies, one of which directed against the N-terminal portion of FGF23. The evaluation of the method accuracy involved the fully automated execution of a dilution test on EDTA plasma from 5 subjects who had not received any monoclonal antibody (mAb), 20 EDTA plasma from patients treated with Burosumab, and 2 EDTA plasma from subjects who had not received any mAb in witch an adequate volume of Burosumab had been added in vitro. One sample with specific diluent (LIAISON® FGF 23) with an adequate volume of Burosumab had been added in vitro. The dilution assay provided highly inaccurate iFGF23 results in samples with therapeutic concentrations of Burosumab and in samples with concentrations below the LoQ (6.5 pg/mL). The addition of Burosumab to the diluent did not produce any analytical interference. Dissociation of iFGF23 from the mAb-target complex in diluted sample could explain the loss of accuracy in the iFGF23 immunoassay using the Liaison XL analyzer. Burosumab could be an interferent in immunoassay procedures of iFGF23.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"89-105"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of diagnostic performances of Pro-neurotensin and Heart-type fatty acid binding protein as reliable biomarkers for cardiovascular diseases. 评估前神经紧张素和心脏型脂肪酸结合蛋白作为心血管疾病可靠生物标记物的诊断性能。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-02 Epub Date: 2024-11-26 DOI: 10.1080/15321819.2024.2430332

Ahmed Shaker Elzantout, Amal Ahmed Mohamed, Manar Selim Fouda, Gamil Karam Mohamed, George Ghaly Girgis, Nesreen Hamdy Mahmoud, Mohamed Abdel Kader Elian, Mariana Victor Philips, Rasha Mohamed, Mohamed Moustafa Omran

{"title":"Evaluation of diagnostic performances of Pro-neurotensin and Heart-type fatty acid binding protein as reliable biomarkers for cardiovascular diseases.","authors":"Ahmed Shaker Elzantout, Amal Ahmed Mohamed, Manar Selim Fouda, Gamil Karam Mohamed, George Ghaly Girgis, Nesreen Hamdy Mahmoud, Mohamed Abdel Kader Elian, Mariana Victor Philips, Rasha Mohamed, Mohamed Moustafa Omran","doi":"10.1080/15321819.2024.2430332","DOIUrl":"10.1080/15321819.2024.2430332","url":null,"abstract":"Aim: In 2019, cardiovascular diseases accounted for 32% of global deaths. So, early detection of cardiac disorders is crucial. The study aimed to examine the suitability of Pro-neurotensin and Heart-type fatty acid binding protein as dependable biomarkers for cardiac patients with Heart failure as a primary diagnosis.Methodology: The prospective study involved 204 Egyptian volunteers (100 cardiac patients and 104 controls) enrolled from El-Sahel Teaching Hospital in Cairo, Egypt, between October 2022 and May 2023. Inclusion criteria included a high risk of cardiovascular events with symptoms like a fast or irregular pulse, shortness of breath, and fatigue. Exclusion criteria included asymptomatic individuals, cognitive disorders, and psychiatric conditions. The Research Ethics Committee approved the protocol. The consultant conducted a clinical examination of all patients and assessed their heart state. Serum ProNT and H-FABP were detected using a kit for the sandwich ELISA technique.Results: ProNT and H-FABP were significantly elevated in patients compared to controls with p < 0.001. Demonstrated sensitivity of 81% and 84%, with a specificity of 89% and 91%, respectively.Conclusion: Elevated ProNT and H-FABP levels are associated with severe CVDs, suggesting their potential as diagnostic biomarkers for patients, specifically those with heart failure, as a primary characteristic.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"49-74"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Further evidence of antibodies against Crimean-Congo haemorrhagic fever virus in different livestock species in Nigeria. 尼日利亚不同牲畜物种中克里米亚-刚果出血热病毒抗体的进一步证据。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-02 Epub Date: 2024-11-11 DOI: 10.1080/15321819.2024.2426146

Chinonyerem Chinyere, Ismaila Shittu, Ndudim Ogo, Adeyinka Adedeji, Aliyu Sada, Vakuru Columba, Hussaini Ularamu, Chika Nwosuh, Clement Meseko

引用次数: 0

Rotavirus-specific-IgA and cytokines responses in Ascaris lumbricoides-infected preschool-aged Nigerian children following rotavirus vaccination. 尼日利亚学龄前蛔虫感染儿童接种轮状病毒疫苗后的轮状病毒特异性-IgA 和细胞因子反应。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-02 Epub Date: 2024-11-12 DOI: 10.1080/15321819.2024.2426147

Kazeem Sanjo Akinwande, Paul Akinniyi Akinduti, OlatunbosunGaniyu Arinola

{"title":"Rotavirus-specific-IgA and cytokines responses in Ascaris lumbricoides-infected preschool-aged Nigerian children following rotavirus vaccination.","authors":"Kazeem Sanjo Akinwande, Paul Akinniyi Akinduti, OlatunbosunGaniyu Arinola","doi":"10.1080/15321819.2024.2426147","DOIUrl":"10.1080/15321819.2024.2426147","url":null,"abstract":"Rotavirus diarrhea and Ascaris lumbricoides (Al) infection increase intestinal morbidity and were associated with altered immune responses that compromise the vaccine efficacy in children. The serum level of rotavirus specific IgA (RV-IgA) and cytokine profiles in A. lumbricoides (AI) infected preschool-aged Nigerian children were estimated following oral rotavirus vaccination. Nineteen of the 149 preschool-aged children (aged 6 to 60 months) with Ascaris lumbricoides infection paired with age and sex-matched helminth - free children were administered with oral rotavirus vaccine after intestinal helminth screening using stool sample concentration technique. Separated sera from 3 mL venous blood samples were collected and estimated for cytokines (IFN-γ, TNF-α, IL-4, IL-8 IL-6, IL-10) and RV-IgA before and three weeks after rotavirus vaccination using Enzyme Linked Immunosorbent Assay. IFN-γ, IL-8, IL-4 were significantly lower at post-vaccination in Al-infected children compared with pre-vaccination. Serum IL-10 was significantly higher at post-vaccination in both Al-infected children and helminth-free controls, compared with pre-vaccination levels (p < 0.05). Pre-vaccination IL-8 and IL-6 were significantly higher in Ascaris lumbricoides-infected children, while the post-vaccination IL-8 was significantly higher in Ascaris lumbricoides-infected compared with control. At post-vaccination period, RV-IgA level was lower in Al-infected children and significantly higher in helminth - free control group compared to pre-vaccination RV-IgA level. Ascaris lumbricoides infection contributed to down-regulation of some cytokines and antibody responses to oral rotavirus vaccine.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"75-88"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0