Journal of immunoassay & immunochemistry最新文献_第2页

Development of cashew-alginate microbeads and powdered dose forms: prospects for oral vaccine delivery in chickens. 腰果海藻酸微珠和粉末剂型的开发：鸡口服疫苗的前景。

Journal of immunoassay & immunochemistry Pub Date : 2024-11-01 Epub Date: 2024-08-21 DOI: 10.1080/15321819.2024.2393184

Olawale Olawumi Ola, Benjamin Obukowho Emikpe, Noble Kuntworbe, Michael Ayodele Odeniyi, Theophilus Aghogho Jarikre, Opeyemi Mayowa Onilude, Yaa Asantewaa Osei, Derrick Adu Asare

{"title":"Development of cashew-alginate microbeads and powdered dose forms: prospects for oral vaccine delivery in chickens.","authors":"Olawale Olawumi Ola, Benjamin Obukowho Emikpe, Noble Kuntworbe, Michael Ayodele Odeniyi, Theophilus Aghogho Jarikre, Opeyemi Mayowa Onilude, Yaa Asantewaa Osei, Derrick Adu Asare","doi":"10.1080/15321819.2024.2393184","DOIUrl":"10.1080/15321819.2024.2393184","url":null,"abstract":"Conventional oral vaccine delivery in poultry is challenging due to vaccine degradation in the gastrointestinal (GI) environment and the need for cold-chain storage. Microencapsulation offers a solution by protecting vaccines from GI degradation and improving stability. Natural polymers like alginate and cashew gum have mucoadhesive properties, making them promising candidates for oral vaccine delivery. This study developed cashew-alginate microbeads and a powdered dose form for oral vaccine delivery in chickens. The microbeads were created using ionotropic gelation, while the powdered form was obtained via freeze-drying. These formulations were characterized for size, shape, and stability using scanning electron microscopy (SEM), light microscopy, X-ray diffraction (XRD), and Energy Dispersive X-ray (EDX). Peak adhesion time (PAT) was determined using chicken intestinal and esophageal tissues, and antigenicity was assessed with in-vitro hemagglutination (HA) and hemagglutination inhibition (HI) assays. The microbeads exhibited a spherical shape with a porous structure, suggesting enhanced antigen accommodation. Hemagglutination Inhibition tests indicated that the experimental vaccine remained effective without cold-chain storage for three months. These findings suggest that cashew-alginate microbeads are promising for oral vaccine delivery in poultry.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"549-565"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142017706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-Acetyltransferase 2 gene polymorphism and its serum levels in vitiligo patients. 白癜风患者的 N-乙酰转移酶 2 基因多态性及其血清水平。

Journal of immunoassay & immunochemistry Pub Date : 2024-11-01 Epub Date: 2024-10-15 DOI: 10.1080/15321819.2024.2415898

Heba A S Bazid, Mostafa A Hammam, Mona H Keshk, Mohammed L Mostafa, Eman M Abd El Gayed

{"title":"N-Acetyltransferase 2 gene polymorphism and its serum levels in vitiligo patients.","authors":"Heba A S Bazid, Mostafa A Hammam, Mona H Keshk, Mohammed L Mostafa, Eman M Abd El Gayed","doi":"10.1080/15321819.2024.2415898","DOIUrl":"10.1080/15321819.2024.2415898","url":null,"abstract":"Background: Although numerous mechanisms are involved in vitiligo pathogenesis, few studies correlate N-acetyltransferase 2 to this disease.Aim: To assess the N-acetyltransferase 2 (rs1799929) gene and its serum levels in vitiligo patients.Subjects and methods: In this case-control study, 65 vitiligo cases were compared to 65 age- and sex-matched healthy controls. Serum NAT2 levels and the NAT2 gene polymorphism (rs1799929) were evaluated using ELISA and real-time PCR, respectively.Results: Serum N-acetyltransferase 2 levels were significantly lower in cases than in controls, 1.24 ± 0.31 vs. 2.01 ± 0.46 (p = 0.001). CC genotype was more dominant in controls (58.5%) than in cases (20%). TT and CT genotypes were more dominant in cases (30.8% and 49.2%) than in controls (13.8% and 27.7%), respectively (p = 0.001). The C allele was more prominent in controls (72.3%) than in cases (44.6%) while the T allele was more dominant in cases (55.4%) than in controls (27.7%) (p = 0.001). N-acetyltransferase 2 slow acetylator phenotype (TT genotype) was higher in cases (30.8%) than in controls (13.8%) and rapid acetylator phenotypes (CC and CT genotypes) were higher in controls (86.2%) than in cases (69.2%) (p = 0.035).Conclusion: Slow acetylator genotype (TT) of NAT2 gene (rs1799929) and low serum levels of NAT2 enzyme might play a role in the susceptibility and pathogenesis of vitiligo.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"518-528"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum collagen IV as a predictor for response to direct-acting antivirals hepatitis C therapy. 血清胶原蛋白 IV 可预测直接作用抗病毒药物治疗丙型肝炎的反应。

Journal of immunoassay & immunochemistry Pub Date : 2024-11-01 Epub Date: 2024-10-14 DOI: 10.1080/15321819.2024.2415882

Mohammed El Behery, AhmedI Elghwab, Ashraf A Tabll, Elsherbiny H Elsayed, Mohamed A Abdelrazek

{"title":"Serum collagen IV as a predictor for response to direct-acting antivirals hepatitis C therapy.","authors":"Mohammed El Behery, AhmedI Elghwab, Ashraf A Tabll, Elsherbiny H Elsayed, Mohamed A Abdelrazek","doi":"10.1080/15321819.2024.2415882","DOIUrl":"10.1080/15321819.2024.2415882","url":null,"abstract":"Althoughchronic hepatitis C (CHC) therapies based on direct-acting antiviral (DAA) agents safely improved treatment effectiveness, some cases do not obtain sustained virological response (SVR) and, thus, evaluating factors that may be related to treatment failure is very important. We aimed to evaluate the association of baseline serum collagen IV with DAA treatment failure in Egyptian patients with CHC. A total of 175 CHC patients (100 responders and 75non-responders tosofosbuvir/daclatasvir) were included. Collagen IV was assessed using sensitive chemiluminescent immunoassay. There was distinctly higher (P < 0.0001) collagen IV in non-responders compared to responder patients as the median (interquartile range) were 19.02 (13.4-25.2) vs.9.7 (7.2-12.3) µg/L, respectively. Collagen IV has a good ability for distinguishing nonresponders from responder patients (AUC = 0.890) with sensitivity of 92%, specificity 72%, PPV 71.1%, NPV 92.3% and accuracy of 80.6%. Collagen IV was correlated (p < 0.05) with decreased albumin (r=-0.266), elevated APRI (r = 0.288), and elevated FIB-4 (r = 0.281) scores. In conclusion,these findings suggested the remarkable role of baseline collagen IV in the prediction of HCV DAAs treatment response. Thus, however further studies are needed, its measurement may improve treatment duration and the disease control.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"539-548"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impacts of host factors on susceptibility to SARS-CoV-2 infection and COVID-19 progression. 宿主因素对 SARS-CoV-2 感染易感性和 COVID-19 进展的影响。

Journal of immunoassay & immunochemistry Pub Date : 2024-11-01 Epub Date: 2024-11-17 DOI: 10.1080/15321819.2024.2429538

Ghada M Salum, Mai Abd El Meguid, Basma E Fotouh, Reham M Dawood

引用次数: 0

Molecular detection and genotyping of HBV from HBsAg positive patients in a tertiary hospital in Nigeria. 尼日利亚一家三甲医院对 HBsAg 阳性患者的 HBV 进行分子检测和基因分型。

Journal of immunoassay & immunochemistry Pub Date : 2024-11-01 Epub Date: 2024-09-02 DOI: 10.1080/15321819.2024.2397377

Adedayo O Faneye, Aisha Mustafa, Babatunde O Motayo, Adewale V Opayele, Kolawole O Akande

{"title":"Molecular detection and genotyping of HBV from HBsAg positive patients in a tertiary hospital in Nigeria.","authors":"Adedayo O Faneye, Aisha Mustafa, Babatunde O Motayo, Adewale V Opayele, Kolawole O Akande","doi":"10.1080/15321819.2024.2397377","DOIUrl":"10.1080/15321819.2024.2397377","url":null,"abstract":"Background: Nigeria remains one of the countries with a high hepatitis B virus (HBV) burden in Africa. Reports have indicated the presence of mixed HBV genotypes in Nigeria; however, there is still paucity of data regarding mixed genotype infections particularly in the Southern part of the country.Objective: Our aim is to determine the HBV genotype distribution among HBsAg-positive gastroenterology patients at the University College Hospital Ibadan, Nigeria.Method: Serum samples were screened for HBsAg by ELISA, and positive samples were genotyped by semi-nested multiplex PCR for HBV genotypes A, B, C, D, E and F.Results: Data generated were analyzed in R-studio. A total of 81/90 (90%) of HBsAg-positive samples were successfully genotyped, and genotype A was most prevalent with 15.7%, while genotypes B and E were the least with 1.2% each. Genotypes A/C infection was the highest among mixed infections with 40% prevalence, while genotypes A/D were the least prevalent mixed infection with 4.8%.Conclusion: We advocate for a comprehensive genotype analysis in larger cohorts across Nigeria, to give a more comprehensive understanding of the distribution and prevalence of different HBV genotypes population wide.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"529-538"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 更正。

Journal of immunoassay & immunochemistry Pub Date : 2024-09-02 Epub Date: 2024-07-10 DOI: 10.1080/15321819.2024.2377505

引用次数: 0

Detection of JC and BK polyomaviruses in patients with colorectal cancer (CRC) by PCR. 通过 PCR 检测结直肠癌 (CRC) 患者体内的 JC 和 BK 多瘤病毒。

Journal of immunoassay & immunochemistry Pub Date : 2024-09-02 Epub Date: 2024-08-05 DOI: 10.1080/15321819.2024.2384581

Mahboube Farbarin, Hoorieh Soleimanjahi, Bita Bakhshi, Zeinab Nasiri, Kamal Fakhredini

{"title":"Detection of JC and BK polyomaviruses in patients with colorectal cancer (CRC) by PCR.","authors":"Mahboube Farbarin, Hoorieh Soleimanjahi, Bita Bakhshi, Zeinab Nasiri, Kamal Fakhredini","doi":"10.1080/15321819.2024.2384581","DOIUrl":"10.1080/15321819.2024.2384581","url":null,"abstract":"Background: Overall, 20-30% of all cancers are estimated to be linked to infectious agents. Polyomaviruses are oncogenic cause in rodent models, readily transform their cells, and cause chromosomal instability in animal and human cells in-vitro. Some reports have indicated the presence of JCPyV and BKPyV in some human tumors. The JCPyV and BKPyV genome encodes some transforming proteins such as LT-Ag. Thus, these viruses could cause or promote some neoplasia, such as lymphomas, pancreatic, prostate, and colorectal cancers. Colorectal cancer (CRC) is the third most common cancer in the world. Risk factors for developing CRC are associated with personal features or habits, such as age, lifestyle, and gut microbiota.Materials and methods: In this study, we examined the prevalence of JCPyV and BKPyV in the 23 fecal samples of CRC patients and 24 healthy samples (control group). Virus DNA was extracted by a Favorgen DNA extraction kit. The large T antigen of JCPyV and VP1 of BKPyV were investigated by optimized multiplex PCR.Results: One of the samples was positive for the JCPyV (4.3%), while in the samples of healthy individuals, the JCPyV was negative. Also, positive results for BKPyV PCR were obtained for five cases (21.7%) in the samples of the CRC group and one case (4.1%) in healthy individuals.Conclusion: The result showed no direct correlation between tumorigenesis and polyomavirus infections in CRC development. However, the exact role of BKPyV and JCPyV is still controversial and needs further study with larger sample size.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"467-480"},"PeriodicalIF":0.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical study of ZNF76 rs10947540 and SCUBE3 rs1888822 single nucleotide polymorphisms in the Egyptian patients with systemic lupus Erythematosus. 埃及系统性红斑狼疮患者 ZNF76 rs10947540 和 SCUBE3 rs1888822 单核苷酸多态性的生化研究。

Journal of immunoassay & immunochemistry Pub Date : 2024-09-02 Epub Date: 2024-07-09 DOI: 10.1080/15321819.2024.2371590

Mohamed Farag Ali Assar, Eman Masoud Abd El Gayed, Amal Salah Abd El-Hamid Ewis, Ahmed B Zaid, Eman Abd Allah Mahmoud Fouda

{"title":"Biochemical study of ZNF76 rs10947540 and SCUBE3 rs1888822 single nucleotide polymorphisms in the Egyptian patients with systemic lupus Erythematosus.","authors":"Mohamed Farag Ali Assar, Eman Masoud Abd El Gayed, Amal Salah Abd El-Hamid Ewis, Ahmed B Zaid, Eman Abd Allah Mahmoud Fouda","doi":"10.1080/15321819.2024.2371590","DOIUrl":"10.1080/15321819.2024.2371590","url":null,"abstract":"Systemic lupus erythematosus (SLE) is a common autoimmune disease marked by the formation of apoptotic debris and the presence of autoantibodies that target nuclear components. At this moment, the actual cause of SLE is uncertain. Genetic variables have been well proven to have a significant role in the propensity of SLE. This study aimed to investigate the effect of (ZNF76) rs (10947540) and (SCUBE) rs (1888822) gene polymorphism in patients with systemic lupus erythematosus. A case control study has been carried out at Medical Biochemistry & Molecular biology and Rheumatology unit of Internal Medicine Departments, Faculty of Medicine, Menoufia University, Egypt, for 1-year duration between 1 June 2022 and 1 June 2023. Sixty patients were females (75%) and twenty patients were males (25%). Their ages ranged from 19 to 53 years. Their disease durations ranged from 7 months to 20 years. The findings indicated that the TC genotype of the ZNF76 rs10947540 gene increases the risk of SLE by 2.274-fold, while the dominant TC + CC increases the risk by 2.472-fold, and the C allele increases the risk by 2.115-fold. Additionally, the results showed that the TT genotype of the SCUBE3 rs1888822 gene increases the risk of SLE by 3.702-fold, the dominant GT + TT increases the risk by 2.304-fold, and the T allele increases the risk by 2.089-fold, while the GT genotype increases the risk by 1.918-fold. The study revealed significant associations between the genotypes of these polymorphisms and certain clinical parameters in SLE patients. These findings highlight the potential genetic contributions to SLE susceptibility and its clinical manifestations, providing valuable insights for future research and potential personalized approaches to the management of this complex autoimmune disease.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"415-431"},"PeriodicalIF":0.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141563507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interferon-induced protein 44 (IFI44) and interferon regulatory factor 4 (IRF4) gene expression in rheumatoid arthritis. 类风湿性关节炎中的干扰素诱导蛋白 44 (IFI44) 和干扰素调节因子 4 (IRF4) 基因表达。

Journal of immunoassay & immunochemistry Pub Date : 2024-09-02 Epub Date: 2024-07-25 DOI: 10.1080/15321819.2024.2381524

Shaimaa Elsayed Ramadan Genena, Maha A F Hamouda, Norhan M Salama, Enas S Zahran, Asmaa A Abdel Latif, Ashraf A Dawood

{"title":"Interferon-induced protein 44 (IFI44) and interferon regulatory factor 4 (IRF4) gene expression in rheumatoid arthritis.","authors":"Shaimaa Elsayed Ramadan Genena, Maha A F Hamouda, Norhan M Salama, Enas S Zahran, Asmaa A Abdel Latif, Ashraf A Dawood","doi":"10.1080/15321819.2024.2381524","DOIUrl":"10.1080/15321819.2024.2381524","url":null,"abstract":"Background and objectives: The type I interferon (IFN) signature has been found to be overactivated in many systemic autoimmune diseases. This may be explained by impaired regulation of interferon-stimulated genes (ISGs) as well as interferon-induced protein 44 (IFI44) expression via their regulatory mechanisms via interferon regulatory factors (IRFs).Patients and methods: This case-control study includes two groups: 50 RA patients and 50 healthy controls. The quantification of IFI44 and IRF4 expression levels by the real-time PCR technique was estimated. Disease Activity Score-28 (DAS-28) was estimated for RA patients only.Results: Among the RA patients, there were statistically significant increased ESR, CRP, TLC, RF, and anti-CCP levels (p value < 0.001) and significant increased expression of the IFI44 and IRF4 genes (p value < 0.001). There was a significant positive correlation between the IFI44 and IRF4, and there was a significant correlation between both and ESR and anti-CCP among RA patients. At a cutoff point of 1.95, IFI44 shows higher sensitivity and specificity values than IRF4 for the diagnosis of RA.Conclusion: IFI44 was more sensitive for RA diagnosis than IRF4. IFI44 and IRF4 overexpression could be promising predictors of RA diagnosis and might become useful clinical tools to guide therapeutic strategies.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"432-451"},"PeriodicalIF":0.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The value of immunohistochemical expression of SOX9 and CD34 in alopecia areata. SOX9和CD34的免疫组化表达在斑秃中的价值。

Journal of immunoassay & immunochemistry Pub Date : 2024-09-02 Epub Date: 2024-07-23 DOI: 10.1080/15321819.2024.2383676

Heba A S Bazid, Alaa H Marae, Bassant Farag, Rania Abdallah Abdallah

{"title":"The value of immunohistochemical expression of SOX9 and CD34 in alopecia areata.","authors":"Heba A S Bazid, Alaa H Marae, Bassant Farag, Rania Abdallah Abdallah","doi":"10.1080/15321819.2024.2383676","DOIUrl":"10.1080/15321819.2024.2383676","url":null,"abstract":"Background: Alopecia areata (AA), an immune-mediated disorder, is marked by temporary, nonscarring hair loss. The bulge area is protected from immune attacks by immune privilege; however, recent studies demonstrated immune cells infiltrating the bulge area.Objective: This study aims to investigate the immunohistochemical expression of the sex-determining region Y-box 9 (SOX9) and cluster of differentiation 34 (CD34) in AA patients as markers of hair follicle stem cells (HFSCs) and progenitor cells, respectively.Methods: Immunohistochemical staining of SOX9 and CD34 was applied on skin samples of 20 AA patients and 20 healthy controls.Results: SOX9 and CD34 were significantly lower in lesional samples of cases compared to perilesional and control skin biopsies. Furthermore, SOX9 level was negatively correlated with the severity of alopecia tool score (SALT score) among the studied AA patients. Moreover, lowered SOX9 expression was present in patients with recurrent attacks.Conclusions: The significant reduction of stem cell markers (SOX9 and CD34) in our studied AA cases signifies the pathological affection of HFSCs and their progeny in AA. This is thought to cause a loss of competence in generating new hair in some AA cases, which needs to be validated in further research.Limitations of the study: This study has a small sample size.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"452-466"},"PeriodicalIF":0.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141748409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0