Journal of immunoassay & immunochemistry最新文献_第3页

Cyclooxygenase-2 (COX-2) and hypoxia-induced factor 1α (HIF1α) expression in colorectal carcinoma and its correlation with other clinicopathological parameters. 环氧化酶-2 （COX-2）和缺氧诱导因子1α (HIF1α)在结直肠癌中的表达及其与其他临床病理参数的相关性

Journal of immunoassay & immunochemistry Pub Date : 2026-01-01 Epub Date: 2026-05-20 DOI: 10.1080/15321819.2026.2676887

Mayada Saad Farrag, Amira Fikry Mohammed AboSamra, Marwa Hosny Mohamed Khashaba, Azza Hassan Mohammad Zidan

{"title":"Cyclooxygenase-2 (COX-2) and hypoxia-induced factor 1α (HIF1α) expression in colorectal carcinoma and its correlation with other clinicopathological parameters.","authors":"Mayada Saad Farrag, Amira Fikry Mohammed AboSamra, Marwa Hosny Mohamed Khashaba, Azza Hassan Mohammad Zidan","doi":"10.1080/15321819.2026.2676887","DOIUrl":"10.1080/15321819.2026.2676887","url":null,"abstract":"Background: Colorectal cancer is identified as the third-most prevalent cancer globally and the second leading cause of cancer mortality.Methods: This study investigated the immunohistochemical expression of Cyclooxygenase-2 and Hypoxia-Induced Factor 1 alpha & correlated their expression with clinicopathological parameters in 100 retrospectively selected cases of colorectal adenocarcinoma from Port Said Governorate, Egypt.Results: COX-2 was positively expressed in 67% of cases, and HIF-1α was positive in 57%. COX-2 positivity was statistically significantly associated only with lymph node metastasis (p = 0.019). HIF-1α positivity showed significant associations with multiple indicators of aggressive disease, including high tumor grade (p < 0.001), advanced tumor stage (T3-T4, p = 0.047), lymph node metastasis (p < 0.001), and advanced TNM stage (p = 0.006). Crucially, a statistically significant positive correlation was detected between the expression of COX-2 and HIF-1α (p = 0.039).Conclusion: COX-2 and HIF-1α are often expressed in colorectal carcinoma and are significantly associated with aggressive clinicopathological features, notably lymph node metastasis and advanced tumor stage. The significant co-expression of these two markers supports a biological constructive collaboration, suggesting that their simultaneous presence drives tumors toward a more invasive and metastatic phenotype, predicting a poorer prognosis. Detection of COX-2 and HIF-1α is recommended as an effective index for assessing prognosis and identifying high-risk patients.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"279-291"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147981985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sarra Ben Rejeb, MD - Early Career Prize Winner 2025. Sarra Ben Rejeb，医学博士- 2025年早期职业奖得主。

Journal of immunoassay & immunochemistry Pub Date : 2026-01-01 Epub Date: 2026-06-25 DOI: 10.1080/15321819.2026.2691337

引用次数: 0

Quantitative live-imaging platform to probe the spatio-temporal internalization and lysosomal degradation of therapeutic antibodies and ADCs in cells. 定量实时成像平台，探索治疗性抗体和adc在细胞中的时空内化和溶酶体降解。

Journal of immunoassay & immunochemistry Pub Date : 2026-01-01 Epub Date: 2026-06-12 DOI: 10.1080/15321819.2026.2681542

Nikhil Jain, Atul Tiwari, Manoj Kumar Gupta, Devika C Namboodiri, Adarsh Ashok Mavinkurve, Maneesh R Pingle

{"title":"Quantitative live-imaging platform to probe the spatio-temporal internalization and lysosomal degradation of therapeutic antibodies and ADCs in cells.","authors":"Nikhil Jain, Atul Tiwari, Manoj Kumar Gupta, Devika C Namboodiri, Adarsh Ashok Mavinkurve, Maneesh R Pingle","doi":"10.1080/15321819.2026.2681542","DOIUrl":"10.1080/15321819.2026.2681542","url":null,"abstract":"The therapeutic efficacy of antibody-drug conjugates (ADCs) is governed not only by target binding but also by the efficiency of receptor-mediated internalization, intracellular trafficking, and lysosomal degradation. However, most conventional assays provide static or endpoint measurements, limiting their ability to resolve these dynamic, multistep processes. Here, we report a quantitative, dual-probe live-cell imaging platform that simultaneously tracks antibody internalization and lysosomal degradation with spatiotemporal resolution. By combining an internalization tracer with a cathepsin-activated fluorogenic sensor, this assay enables direct, real-time measurement of lysosomal routing following cellular uptake. Using trastuzumab and clinically approved HER2-targeting ADCs as model systems, we demonstrate that internalization and lysosomal degradation are temporally uncoupled and occur with distinct kinetics. Moreover, ADCs sharing the same antibody backbone exhibited markedly different processing profiles, highlighting the influence of linker chemistry and payload properties on their intracellular fate. These results underscore the importance of evaluating ADCs as integrated molecular systems rather than as modular components. This platform provides a scalable and mechanistically informative tool for ADC development, enabling candidate ranking, linker screening, and rational payload - linker optimization based on functional intracellular behavior rather than uptake alone. As such, it offers a framework for data-driven design of next-generation ADCs with improved therapeutic index.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"232-246"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148239473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circular RNA TUBD1 and microRNA-146a gene expression as potential biomarkers in immune thrombocytopenic purpura. 环状RNA TUBD1和microRNA-146a基因表达作为免疫性血小板减少性紫癜的潜在生物标志物。

Journal of immunoassay & immunochemistry Pub Date : 2026-01-01 Epub Date: 2026-07-14 DOI: 10.1080/15321819.2026.2702519

Amany M Wahb, Amal Abdelrasoul Alhosary, Nermin I Rizk, Rana K A Wahb, Alaa Osama Nagy, Basma A Mostafa Mohamed

{"title":"Circular RNA TUBD1 and microRNA-146a gene expression as potential biomarkers in immune thrombocytopenic purpura.","authors":"Amany M Wahb, Amal Abdelrasoul Alhosary, Nermin I Rizk, Rana K A Wahb, Alaa Osama Nagy, Basma A Mostafa Mohamed","doi":"10.1080/15321819.2026.2702519","DOIUrl":"10.1080/15321819.2026.2702519","url":null,"abstract":"The multifactorial primary immune thrombocytopenia (ITP) is an acquired autoimmune disease highlighted by dysregulation of immune responses that lead to both increased platelet destruction and impaired platelet production with reduced blood platelet count. The pathophysiology of autoimmune illnesses such as ITP has been linked to regulatory non-coding RNAs, such as circular RNAs (circRNAs) and microRNAs (miRNAs). Our goal was to assess circTUBD1 and miR-146a gene expression in Egyptian ITP patients to correlate them with clinicopathological characteristics. In this study, two groups of forty Egyptian participants were established: twenty individuals diagnosed with chronic ITP and twenty healthy individuals matched in terms of sex and age, recruited from the Internal Medicine department at Menoufia University Hospitals in Egypt. Blood samples were taken from each individual to analyze the plasma expression levels of circTUBD1 and miR-146a using real-time PCR analysis. There was an upregulation of circTUBD1 and a downregulation of MiR-146a levels that showed a significant difference between the control group and the ITP patient group (p < 0.001 for both biomarkers). The expression level of circTUBD1 and MiR-146a exhibits a significant inverse relationship (r = -0.495and p = 0.026). MiR-146a and circTUBD1 can be promising noninvasive biomarkers of chronic ITP and disease activity.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"318-332"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148447470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of a cell-based methodology to evaluate filgrastim relative affinity to granulocyte colony-stimulating factor receptor. 基于细胞的非格昔汀对粒细胞集落刺激因子受体的相对亲和力评价方法的发展和验证。

Journal of immunoassay & immunochemistry Pub Date : 2026-01-01 Epub Date: 2025-12-19 DOI: 10.1080/15321819.2025.2594998

Víctor R Campos-García, Lilia Tierrablanca-Sánchez, Nancy D Ramírez-Ibáñez, Néstor O Pérez, José González-Valdez

{"title":"Development and validation of a cell-based methodology to evaluate filgrastim relative affinity to granulocyte colony-stimulating factor receptor.","authors":"Víctor R Campos-García, Lilia Tierrablanca-Sánchez, Nancy D Ramírez-Ibáñez, Néstor O Pérez, José González-Valdez","doi":"10.1080/15321819.2025.2594998","DOIUrl":"10.1080/15321819.2025.2594998","url":null,"abstract":"Filgrastim, a therapeutic protein for the treatment of neutropenia, exerts its biological activity by interacting with the granulocyte colony-stimulating factor receptor. However, the affinity of filgrastim to its receptor is usually overlooked during characterization and comparability tests, since its biological activity is assessed only by in vitro proliferation assays. In this work, we propose the use of a cell-based colorimetric method to determine the relative affinity of filgrastim to its receptor expressed in murine myeloid leukemia cells. After performing a validation exercise, the method proved to be accurate, specific, and linear within an affinity interval of 75 to 130%; precise with a confidence interval of 93.6 to 114.2% and a coefficient of variation of 12.6%. The validation exercise proved that the proposed cell-based method is a viable alternative to evaluate the biological activity of filgrastim via the affinity for its receptor and it is suitable to be included as part of its biological characterization exercises as well as in comparability exercises for products coming from distinct manufacturing processes.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"60-76"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145794049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pivotal role of interleukin 27 serum level and missense variant (rs181206) in multiple sclerosis. 白介素27血清水平和错义变异（rs181206）在多发性硬化中的关键作用。

Journal of immunoassay & immunochemistry Pub Date : 2026-01-01 Epub Date: 2026-02-04 DOI: 10.1080/15321819.2026.2623279

Amany E Elashkar, Marwa M Khalil, Mona S Elkholy, Eman Samy, Sanaa S Hamam, Amira Samy Elmaghraby

{"title":"Pivotal role of interleukin 27 serum level and missense variant (rs181206) in multiple sclerosis.","authors":"Amany E Elashkar, Marwa M Khalil, Mona S Elkholy, Eman Samy, Sanaa S Hamam, Amira Samy Elmaghraby","doi":"10.1080/15321819.2026.2623279","DOIUrl":"10.1080/15321819.2026.2623279","url":null,"abstract":"The incidence of multiple sclerosis (MS) has increased in recent years. Its pathogenesis involves the interaction between various elements, with interleukin 27 (IL-27) playing a key role in autoimmunity. The presence of the IL-27 receptor on astrocytes emphasizes its involvement in the disease's progression.Purpose: The study aims to investigate possible associations between IL27 rs181206, serum level of IL27, and the development of MS.Methods: The study comprised 70 MS patients and 70 seemingly healthy controls. They were genotyped for IL27 rs181206 using the Taqman allelic discrimination approach, and their serum IL27 levels were estimated using ELISA.Results: The frequency of TT genotype, T allele, and IL27 serum level were significantly higher among MS patients compared to controls. There was no significant difference between IL27 serum levels among different genotypes in both MS patients and controls; however, individuals with TT genotype showed higher levels of IL27 than those with CC genotype.Conclusion: TT genotype and T allele can increase the risk of developing MS. On the other hand, carrying the C allele may be associated with a lower risk of MS development. Understanding IL27 genetics and epistatic interactions can help clarify IL27's role in MS pathogenesis and utilize it as a therapeutic target.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"113-128"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146119150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of MALAT1 rs619586 and rs3200401 single-nucleotide polymorphisms with breast cancer risk in Egyptian women. 埃及女性MALAT1 rs619586和rs3200401单核苷酸多态性与乳腺癌风险的关系

Journal of immunoassay & immunochemistry Pub Date : 2026-01-01 Epub Date: 2026-06-16 DOI: 10.1080/15321819.2026.2688806

Sara A El Derbaly, Basma Faried, Eman Hebesh, Samah El-Ghlban

{"title":"Association of MALAT1 rs619586 and rs3200401 single-nucleotide polymorphisms with breast cancer risk in Egyptian women.","authors":"Sara A El Derbaly, Basma Faried, Eman Hebesh, Samah El-Ghlban","doi":"10.1080/15321819.2026.2688806","DOIUrl":"10.1080/15321819.2026.2688806","url":null,"abstract":"Background: MALAT1 (long non-coding RNA) is associated with growth and proliferation of breast cancer (BC). Functional single-nucleotide polymorphisms in MALAT1 may regulate an individual's susceptibility to cancer. Little is known regarding MALAT1 polymorphisms and the risk of BC in Egyptian women.Objectives: This study aims to determine the relationship between MALAT1 polymorphisms (rs619586 and rs3200401) and the risk of BC among Egyptian women, as well as clinicopathology and survival rates.Methods: A case-control study was conducted involving 50 women with confirmed BC and 50 age-matched controls. ELISA was used to measure serum CA 15-3 and carcinoembryonic antigen (CEA). A TaqMan discrimination assay was performed to study MALAT1 SNPs (rs619586 and rs3200401).Results: The G allele at rs619586 and the AG genotype were significantly more prevalent in controls and were associated with a reduced risk of breast cancer. On the other hand, there was no significant link between rs3200401 genotypes and alleles and the risk of BC. The AA genotype of the rs619586 was associated with higher tumor grade.Conclusion: These findings suggest an association between rs619586 and the risk of BC in Egyptian women; however, larger, multicenter studies evaluating functional effects should be conducted.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"247-263"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148264870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Burcu Kök Kendirlioğlu, MD, PhD -Early Career Prize Winner 2024. Burcu Kök Kendirlioğlu，医学博士，博士- 2024年早期职业奖得主。

Journal of immunoassay & immunochemistry Pub Date : 2025-04-20 DOI: 10.1080/15321819.2025.2496044

引用次数: 0

Evaluation of Ki67 in pathological prognostic staging of breast cancer: a tertiary care center study. Ki67在乳腺癌病理预后分期中的评价：一项三级保健中心研究。

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2025-01-15 DOI: 10.1080/15321819.2025.2451211

Rashmi Wankhade, Arvind Bhake, Nandkishor Bankar, Yugeshwari Tiwade

{"title":"Evaluation of Ki67 in pathological prognostic staging of breast cancer: a tertiary care center study.","authors":"Rashmi Wankhade, Arvind Bhake, Nandkishor Bankar, Yugeshwari Tiwade","doi":"10.1080/15321819.2025.2451211","DOIUrl":"10.1080/15321819.2025.2451211","url":null,"abstract":"Background: The rising global burden of breast cancer demands early detection and effective treatment, with a focus on prognostic and predictive markers. The eighth edition of the American Joint Committee on Cancer staging manual introduced a new prognostic staging system to increase the predictive power of the existing anatomical staging system of breast cancer. The current study aimed to establish the correlation between Ki67 expression with molecular subtypes and with the pathological prognostic stage of invasive ductal carcinoma.Materials and methods: A total of 40 patients were included in the study with samples from 32 modified radical mastectomies and 8 biopsies. Hematoxylin and Eosin staining, histopathological analysis and Ki67 immunostaining were conducted. Descriptive and inferential statistical analyses were performed.Results: Bloom Richardson Grade II was the predominant histological grade. In Grade II cases, 15 of 24 had a Ki67 labeling index of 26-45%, while 6 exceeded 45% (p = 0.001). Pathological prognostic staging reclassified 27 cases, with 24 (75%) downstaged, 3 (9.38%) upstaged, and 5 (15.63%) retaining their clinical stage.Conclusions: Ki67 immunohistochemistry is an effective tool for assessing proliferative activity of invasive ductal carcinoma, aiding in pathological prognostic stage stratification and offering insights into tumor biology.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"186-200"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparison between different chemical fractionation methods for immunoglobulin preparation. 制备免疫球蛋白不同化学分馏方法的比较。

Journal of immunoassay & immunochemistry Pub Date : 2025-03-04 Epub Date: 2025-01-11 DOI: 10.1080/15321819.2025.2450664

Abbasali Salarifar, Pooria Safarzadeh Kozani, Mohammad Javad Rasaee

{"title":"A comparison between different chemical fractionation methods for immunoglobulin preparation.","authors":"Abbasali Salarifar, Pooria Safarzadeh Kozani, Mohammad Javad Rasaee","doi":"10.1080/15321819.2025.2450664","DOIUrl":"10.1080/15321819.2025.2450664","url":null,"abstract":"Background: Application of antibodies in therapeutics and diagnostics are growing Continually. Herein, we aimed to find the most qualified immunoglobulin (Ig) chemical preparation method.Methods: A rabbit was immunized against recombinant SARS-CoV-2 nucleocapsid (NP) and reactive polyclonal antibodies were prepared using the ammonium sulfate (AS), caprylic acid (CA), polyethylene glycol (PEG), and caprylic acid/ammonium sulfate (CA/AS) methods. Different antibody solutions were analyzed by SDS-PAGE and subsequently quantified by ImageJ software for further analysis in terms of Ig purity, Ig recovery, and albumin impurity. Ultimately, the prepared antibodies were assessed via Western blotting and ELISA to evaluate their ability to bind NP.Results: Prepared Ig solutions via the CA/AS method had the highest Ig purity (followed by CA, PEG, and AS) and lowest albumin impurity (followed by CA, AS, and PEG). The PEG method had the highest recovery followed by AS, CA, and CA/AS methods. Moreover, antibodies prepared via different methods demonstrated comparable binding capacities to NP in ELISA and Western blotting.Conclusions: CA/AS, closely followed by CA, proved to be the most qualified method for the preparation of Ig yielding the highest Ig purity while the PEG method resulted in the highest Ig recovery rate.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"169-185"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0