Journal of immunoassay & immunochemistry最新文献_第3页

Impact of CD34/CD309 expression in circulating endothelial progenitor cells on prognosis and response to bortezomib therapy in multiple myeloma. 循环内皮祖细胞中 CD34/CD309 的表达对多发性骨髓瘤预后和硼替佐米治疗反应的影响

Journal of immunoassay & immunochemistry Pub Date : 2024-09-02 Epub Date: 2024-08-13 DOI: 10.1080/15321819.2024.2388614

Nadia ElMenshawy, Manal Ibrahim Fouda, Mohamed Mofreh, Heba Hisham El-Etriby, Manal O Elnenaei, Mohamed Eissa

{"title":"Impact of CD34/CD309 expression in circulating endothelial progenitor cells on prognosis and response to bortezomib therapy in multiple myeloma.","authors":"Nadia ElMenshawy, Manal Ibrahim Fouda, Mohamed Mofreh, Heba Hisham El-Etriby, Manal O Elnenaei, Mohamed Eissa","doi":"10.1080/15321819.2024.2388614","DOIUrl":"10.1080/15321819.2024.2388614","url":null,"abstract":"Multiple myeloma (MM) is a prevalent yet incurable hematologic malignancy. Despite the proven efficacy of proteasome inhibitors in treating MM, resistance to Bortezomib-based treatments persists in a subset of patients. This case control study explores the potential of circulating endothelial progenitor cells (EPCs) as biomarkers for predicting response to Proteasome Inhibitor based therapy combined with Dexamethasone in MM patients. This study was conducted on 105 MM patients receiving bortezomib plus dexamethasone therapy and 90 healthy individuals as a control group. Utilizing 8-color multi-parameter flow cytometry, we assessed the levels of circulating EPCs, identified through CD34 FITC and CD309 PE markers at diagnosis and after one treatment cycle (4 weeks). Our findings revealed that patients exhibiting poor response to therapy showed significantly higher CD34/CD309 values than those with a good response (p < 0.001). The delineation of response based on CD34/CD309 expression was established with a cutoff ≤ 0.9 for percentage (yielding 100% sensitivity and 94.1% specificity) and ≤ 12.5 for absolute value (also with 100% sensitivity and 94.1% specificity). These results underscore the potential of EPC population levels, as quantified by CD34/CD309, to serve as a predictive biomarker for immunomodulatory treatment in MM patients undergoing Proteasome Inhibitor and Dexamethasone therapy.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"481-491"},"PeriodicalIF":0.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular dynamics simulation and purification of chimeric L1/L2 protein from human papillomavirus type 52 expressed in Escherichia coli BL21 (DE3). 在大肠杆菌 BL21 (DE3) 中表达的人乳头瘤病毒 52 型 L1/L2 嵌合蛋白的分子动力学模拟和纯化。

Journal of immunoassay & immunochemistry Pub Date : 2024-09-02 Epub Date: 2024-07-04 DOI: 10.1080/15321819.2024.2376034

Rabiyah Al Adawiah, Apon Zaenal Mustopa, Sri Budiarti, Rifqiyah Nur Umami, Ai Hertati, Herman Irawan, Muh Chaeril Ikramullah, Arwansyah Arwansyah, Jendri Mamangkey, Isti Kartikasari, Huda Salahudin Darusman

{"title":"Molecular dynamics simulation and purification of chimeric L1/L2 protein from human papillomavirus type 52 expressed in Escherichia coli BL21 (DE3).","authors":"Rabiyah Al Adawiah, Apon Zaenal Mustopa, Sri Budiarti, Rifqiyah Nur Umami, Ai Hertati, Herman Irawan, Muh Chaeril Ikramullah, Arwansyah Arwansyah, Jendri Mamangkey, Isti Kartikasari, Huda Salahudin Darusman","doi":"10.1080/15321819.2024.2376034","DOIUrl":"10.1080/15321819.2024.2376034","url":null,"abstract":"The available prophylactic vaccines for human papillomavirus (HPV) in the market are only effective against specific types of HPV, rendering them ineffective for other types of HPV infections. The objective of this research is to investigate the stability of the recombinant protein constructed, namely chimeric L1/L2 protein from HPV type 52, with improved cross-neutralization ability. The 3D model, predicted using Alphafold, Robetta, I-Tasser, and refined with Galaxy Refinement, is validated using Ramachandran plot analysis. The stability is verified through molecular dynamics simulations, considering parameters such as RMSD, RMSF, Rg, and SASA, where stable conditions are observed. The chimeric L1/L2 protein from HPV type 52 is purified using affinity chromatography, and the His-tag is cleaved using SUMO protease to obtain pure chimeric protein with the size of ~ 55 kDa. Western blot analysis confirms binding to anti-L1 HPV type 52 polyclonal antibody. The obtained vaccine candidate can be utilized as an effective prophylactic vaccine against HPV.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"395-414"},"PeriodicalIF":0.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141534549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between matrix metalloproteinase-9-1562C/T gene polymorphism and MMP-9 serum level in rheumatoid arthritis. 类风湿性关节炎患者基质金属蛋白酶-9-1562C/T基因多态性与MMP-9血清水平的关系

Journal of immunoassay & immunochemistry Pub Date : 2024-07-03 Epub Date: 2024-06-11 DOI: 10.1080/15321819.2024.2365699

Farshad Foroughi, Roghaye Keshavarz Sadegh, Maryam Khalaji, Mahin Lashgari, Amir Javadi, Mehdi Sahmani, Shamim Nonejad, Sanaz Keshavarz Shahbaz

{"title":"Association between matrix metalloproteinase-9-1562C/T gene polymorphism and MMP-9 serum level in rheumatoid arthritis.","authors":"Farshad Foroughi, Roghaye Keshavarz Sadegh, Maryam Khalaji, Mahin Lashgari, Amir Javadi, Mehdi Sahmani, Shamim Nonejad, Sanaz Keshavarz Shahbaz","doi":"10.1080/15321819.2024.2365699","DOIUrl":"10.1080/15321819.2024.2365699","url":null,"abstract":"Background: Rheumatoid arthritis (RA) is an autoimmune disease indicated by joint inflammation and cartilage destruction. Matrix metalloproteinase (MMP) enzymes play an influential role in inflammation by affecting the invasion and degradation of anatomical barriers. In this way, the current study investigated the relationship between the MMP-9-1562C/T gene polymorphism and this enzyme's serum level in RA.Methods: The serum levels of MMP-9 in RA patients and healthy controls were measured using the enzyme-linked immunosorbent assay (ELISA). RA was confirmed using rheumatoid factor (RF), anti-cyclic citrullinated peptide (anti-CCP), and C-reactive protein (CRP). Then the MMP-9-1562C/T gene polymorphism was analyzed utilizing polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Also, multivariate analysis investigated the connection between this polymorphism and the risk of RA.Results: In this study, the increase of MMP-9 in patients due to the development of single nucleotide polymorphism in the promoter region of this gene (-1562 C→T) was confirmed by increasing the frequency of heterozygous genotype (CT). Logistic regression analysis also demonstrated that the chance of development of RA is higher in people with CT/CC genotype than in other alleles.Conclusions: We demonstrated that MMP-9-1562C/T gene polymorphism can play a significant role in the occurrence of RA.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"362-381"},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is overexpression of CD163 and CD47 in tumour cells of breast carcinoma implicated in the recruitment of tumour-associated macrophages (TAMs) in tumour microenvironment? immunohistochemical prognostic study. 乳腺癌肿瘤细胞中 CD163 和 CD47 的过度表达是否与肿瘤微环境中肿瘤相关巨噬细胞（TAMs）的招募有关？

Journal of immunoassay & immunochemistry Pub Date : 2024-07-03 Epub Date: 2024-05-30 DOI: 10.1080/15321819.2024.2358879

Marwa Mohammed Dawoud, Hayam Abd El Samie Aiad, Norhan Safwat Kasem, Enas Abu-Bakr El Khouly, Dalia Rifaat Al-Sharaky

{"title":"Is overexpression of CD163 and CD47 in tumour cells of breast carcinoma implicated in the recruitment of tumour-associated macrophages (TAMs) in tumour microenvironment? immunohistochemical prognostic study.","authors":"Marwa Mohammed Dawoud, Hayam Abd El Samie Aiad, Norhan Safwat Kasem, Enas Abu-Bakr El Khouly, Dalia Rifaat Al-Sharaky","doi":"10.1080/15321819.2024.2358879","DOIUrl":"10.1080/15321819.2024.2358879","url":null,"abstract":"Background: Now, targeted therapy and immunotherapy are promoted. tumour -Associated Macrophages (TAMs) are an essential component of immune-response in breast cancer(BC) with prognostic controversy. Additionally, their recruiting factors are still obscure. Purpose:This study aimed to evaluate the prognostic significance of CD163 and CD47 in BC of No Special Type (BC-NST) and to explore their suggested role in recruiting TAMs.Material and methods: This immunohistochemical study was conducted on 91 archival specimens of breast cases. Immunoreactivity scores were correlated with TAMs density, clinicopathological data, and survival.Results: Revealed the highest CD163 expression was detected in the pure DCIS group (p = 0.016), while the highest CD47 expression and high TAMs density were reported in the invasive group (p = 0.008, and p = 0.002 respectively) followed by the DCIS group. In IC-NSTs the CD163 and CD47 scores were associated with poor prognostic parameters like(high grade, advanced stage, distant metastasis, ER negativity,Ki67 index, post-surgical chemotherapy, poor NPI group, high mitotic count, dense infiltration of TAMs, shorter OS). Also, CD47 was associated with the dens infiltration of TAMs in DCIS (p = 0.001). There was a significant correlation between tumour cell expression of CD163 and CD47 in IC-NSTs and DCIS (p = 0.002 and p = 0.009 respectively).Conclusions: High CD163 and CD47 expressions in both DCIS andIBC are intimately associated, significantly associated with poor prognosis and are important provoking factors of TAMs.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"342-361"},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141179940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of scFv-anti-CHIKV-E2 in Escherichia coli with chaperones Co-expression, and its functional assay by electrochemical immunosensor. 在大肠杆菌中与伴侣素共同表达 scFv-抗-CHIKV-E2，并利用电化学免疫传感器对其进行功能检测。

Journal of immunoassay & immunochemistry Pub Date : 2024-07-03 Epub Date: 2024-05-22 DOI: 10.1080/15321819.2024.2356639

Shabarni Gaffar, Siti Hesti Nurbayanti, Yeni Wahyuni Hartati, Mia Tria Novianti, Korry Novitriani, Safri Ishmayana, Muhammad Yusuf, Toto Subroto

{"title":"Expression of scFv-anti-CHIKV-E2 in Escherichia coli with chaperones Co-expression, and its functional assay by electrochemical immunosensor.","authors":"Shabarni Gaffar, Siti Hesti Nurbayanti, Yeni Wahyuni Hartati, Mia Tria Novianti, Korry Novitriani, Safri Ishmayana, Muhammad Yusuf, Toto Subroto","doi":"10.1080/15321819.2024.2356639","DOIUrl":"10.1080/15321819.2024.2356639","url":null,"abstract":"Single Chain Variable Fragment (scFv), a small fragment of antibody can be used to substitute the monoclonal antibody for diagnostic purposes. Production of scFv in Escherichia coli host has been a challenge due to the potential miss-folding and formation of inclusion bodies. This study aimed to express anti-CHIKV E2 scFv which previously designed specifically for Asian strains by co-expression of three chaperones that play a role in increasing protein solubility; GroEL, GroES, and Trigger Factor. The scFv and chaperones were expressed in Origami B E. coli host under the control of the T7 promoter, and purified using a Ni-NTA column. Functional assay of anti-CHIKV-E2 scFv was examined by electrochemical immunosensor using gold modified Screen Printed Carbon Electrode (SPCE), and characterized by differential pulses voltammetry (DPV) using K3[Fe(CN)6] redox system and scanning microscope electron (SEM). The experimental condition was optimized using the Box-Behnken design. The results showed that co-expression of chaperone increased the soluble scFv yield from 54.405 μg/mL to 220.097 µg/mL (~5×). Furthermore, scFv can be used to detect CHIKV-E2 in immunosensor electrochemistry with a detection limit of 0.74048 ng/mL and a quantification limit of 2,24388 ng/mL. Thus, the scFv-anti-CHIKV-E2 can be applied as a bioreceptor in another immunoassay method.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"307-324"},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of melatonin on disease improvement and serum levels of pro-inflammatory cytokines in patients with non-atypical endometrial hyperplasia. 褪黑素对非典型子宫内膜增生症患者病情改善和血清促炎细胞因子水平的影响。

Journal of immunoassay & immunochemistry Pub Date : 2024-07-03 Epub Date: 2024-06-23 DOI: 10.1080/15321819.2024.2371583

Neda Aslany, Zahra Vahedpour, Habibollah Rahimi, Mohsen Masjedi, Hossein Motedayyen

{"title":"Effects of melatonin on disease improvement and serum levels of pro-inflammatory cytokines in patients with non-atypical endometrial hyperplasia.","authors":"Neda Aslany, Zahra Vahedpour, Habibollah Rahimi, Mohsen Masjedi, Hossein Motedayyen","doi":"10.1080/15321819.2024.2371583","DOIUrl":"10.1080/15321819.2024.2371583","url":null,"abstract":"Background: Endometrial hyperplasia (EH), an abnormal proliferation of the endometrial cells, is considered as one of the most common causes of abnormal uterine bleeding. Previous studies have reported that melatonin plays a fundamental role in disease treatment. This study aimed the comparison of the effects of progesterone, as the most common therapeutic approach, and melatonin with progesterone alone in improvement of non-atypical endometrial hyperplasia (NEH) and changes in pro-inflammatory cytokine levels.Methods: Study population consisted of 40 patients with NEH. Patients were divided into two groups, including 20 subjects treated with melatonin and progesterone and 20 individuals treated with progesterone alone. The blood and endometrial sampling was performed from participants before and after a three-month treatment. The histological examination was microscopically done. The serum levels of tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) were measured using ELISA.Results: There was no significant difference in the diabetes status and mean age between patients treated with progesterone and melatonin and those treated with progesterone alone. The improvement rate in the EH was significantly higher in individuals treated with progesterone and melatonin than those treated with progesterone alone (p < 0.05). Additionally, the patients treated with progesterone and melatonin showed significant increases inIFN-γ and TNF-αlevels compared to the control group (p < 0.001-P < 0.05).Conclusion: Melatonin supplementation has a beneficial effect in the treatment of EH due perhaps to enhance the level of IFN-γ and TNF-α.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"382-394"},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asmaa El-Shershaby, PhD -Early Career Prize Winner 2023 Biography. Asmaa El-Shershaby，博士--2023 年早期职业生涯奖获得者简历。

Journal of immunoassay & immunochemistry Pub Date : 2024-06-14 DOI: 10.1080/15321819.2024.2362529

引用次数: 0

Identifying the association between polymorphisms in the GZMB and IFIH1 genes and psoriasis in Egyptians. 确定埃及人 GZMB 和 IFIH1 基因多态性与银屑病之间的关联。

Journal of immunoassay & immunochemistry Pub Date : 2024-05-03 Epub Date: 2024-05-11 DOI: 10.1080/15321819.2024.2352496

Sara Kamal Rizk, Azza Gaber Antar Farag, Samah El-Ghlban, Israa Salah Eldin Metwally

{"title":"Identifying the association between polymorphisms in the GZMB and IFIH1 genes and psoriasis in Egyptians.","authors":"Sara Kamal Rizk, Azza Gaber Antar Farag, Samah El-Ghlban, Israa Salah Eldin Metwally","doi":"10.1080/15321819.2024.2352496","DOIUrl":"10.1080/15321819.2024.2352496","url":null,"abstract":"Objectives: This study aims to examine whether the genetic variants in the genes for Granzyme B (GZMB) and Interferon Induced with Helicase C domain 1 (IFIH1) were associated with psoriasis.Background: Psoriasis, a papulosquamous skin disease, was initially thought of as a disorder primarily of epidermal keratinocytes but is now recognized as one of the most common immune-mediated disorders. It is caused by the interplay between multiple genetic and environmental risk factors.Subjects and methods: This case-control study has 65 participants with psoriasis and 65 healthy controls. Real-time PCR was used to genotype GZMB (rs8192917) and IFIH1 (rs35667974).Results: Genotype occurrence and allelic spreading for both SNPs are in Hardy - Weinberg equilibrium. The genotype and allele distributions of rs35667974 showed no differences between the studied groups. Regarding rs8192917, compared to Group II, there is a statistically significant rise in the CC genotype and C allele in Group I. Higher PASI scores are detected in the C/C and C/T genotypes more than the T/T genotype. Univariate and multivariate analyses revealed that BMI, catalase, MDA, and rs8192917 (C/C) are associated with psoriasis.Conclusion: GZMB rs8192917 was significantly related to psoriasis risk; its C allele is likewise associated with psoriasis vulnerability. However, our investigation found no link between rs35667974 and psoriasis.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"189-209"},"PeriodicalIF":0.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140908880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asmaa El-Shershaby, PhD -Early Career Prize Winner 2023 Biography. Asmaa El-Shershaby，博士--2023 年早期职业生涯奖获得者简历。

Journal of immunoassay & immunochemistry Pub Date : 2024-05-03 Epub Date: 2024-06-14 DOI: 10.1080/15321819.2024.2362529

引用次数: 0

Serological profile of hepatitis B virus infection among traditional birth attendants and the attending pregnant women in Ibadan, Nigeria. 尼日利亚伊巴丹传统助产士和助产孕妇的乙型肝炎病毒感染血清学概况。

Journal of immunoassay & immunochemistry Pub Date : 2024-05-03 Epub Date: 2024-05-16 DOI: 10.1080/15321819.2024.2355549

Yewande Tolulope Nejo, Olukunle Oluwapamilerin Oluwasemowo, Esther Ibidunni Ajao, Miracle Odunayo Ajala, Oluwatosin Felicia Adedire, Ibrahim Kingsley Adegbite, Boluwaji Favour Esan, Oluwatosin Olajumoke Morakinyo, Ayodele Samuel Ibitoye, Anuoluwapo Beauty Alade

{"title":"Serological profile of hepatitis B virus infection among traditional birth attendants and the attending pregnant women in Ibadan, Nigeria.","authors":"Yewande Tolulope Nejo, Olukunle Oluwapamilerin Oluwasemowo, Esther Ibidunni Ajao, Miracle Odunayo Ajala, Oluwatosin Felicia Adedire, Ibrahim Kingsley Adegbite, Boluwaji Favour Esan, Oluwatosin Olajumoke Morakinyo, Ayodele Samuel Ibitoye, Anuoluwapo Beauty Alade","doi":"10.1080/15321819.2024.2355549","DOIUrl":"10.1080/15321819.2024.2355549","url":null,"abstract":"Although a sizable number of pregnant women patronize Traditional Birth Attendants (TBAs) for deliveries in Nigeria, efforts to prevent or reduce the risk of HBV transmission are not targeted at the TBAs and the pregnant women patronizing them. This may be linked to the dearth of information on the serological profiles of HBV among this cohort. We, therefore, show the serological profiles of HBV among the cohort. One hundred and seventy pregnant women and 91 TBAs participated in this study between May and July 2019. Serological markers of HBV infection were assayed using ELISA. A prevalence of, 8.0% (95% CI: 5.0% - 11.5%) for HBsAg, 0.8% (95% CI: 0.0% - 1.9%) for HBeAg, 2.7% (95% CI: 0.8% - 5.0%) for HBcIgM, 26.1% (95% CI: 20.7% - 31.4%) for anti-HBs, 21.5% (95% CI: 16.5% - 25.4%) for anti-HBe and 67.0% (95% CI: 60.9% - 72.8%) for anti-HBc was found indicating a high percentage of carriers. Although 32 (12.3%) of the entire participants claimed to be fully vaccinated, serological evidence was only detected in 4 (12.5%). The high percentage of carriers and low evidence of vaccination necessitate intensified efforts to ensure that adequate interventions are made available and accessible to the TBAs and the pregnant women patronizing them (including newborn babies).","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"247-260"},"PeriodicalIF":0.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140957287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0