Journal of immunoassay & immunochemistry最新文献_第3页

BRAF V600E protein detection by immunohistochemistry in metastatic colorectal cancer tissue: association with tumor characteristics and KRAS mutation. 转移性结直肠癌组织中BRAF V600E蛋白的免疫组化检测：与肿瘤特征和KRAS突变的关系

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-03-26 DOI: 10.1080/15321819.2025.2484408

Sarra Ben Rejeb, Safia Sakly, Rym Bahloul, Adnen Chouchen

{"title":"BRAF V600E protein detection by immunohistochemistry in metastatic colorectal cancer tissue: association with tumor characteristics and KRAS mutation.","authors":"Sarra Ben Rejeb, Safia Sakly, Rym Bahloul, Adnen Chouchen","doi":"10.1080/15321819.2025.2484408","DOIUrl":"10.1080/15321819.2025.2484408","url":null,"abstract":"Immunohistochemistry (IHC) using the BRAF V600E antibody is a sensitive and specific method for detecting BRAF V600E mutations in colorectal cancer (CRC). Given that BRAF and KRAS mutations are mutually exclusive, this study aimed to assess the expression of BRAF V600E in CRC according to KRAS mutation status. Automated IHC analysis was performed on tissue samples from metastatic CRC patients diagnosed between 2012 and 2022 using the anti-BRAF V600E antibody (GenomeME, IHC600). Positive and negative control tissues were used for validation. Cytoplasmic staining was considered positive, with intensity classified as weak, moderate, or strong. The percentage of positive tumor cells was recorded semi-quantitatively. Thirty-five cases were included.The mean age of patients was 60 years (±12.41), with a male-to-female ratio of 2.9. KRAS mutations were present in 48% of cases. BRAF V600E cytoplasmic staining was observed in 37.1%, with a mean percentage of positive cells of 50% (range: 5%-100%). Diffuse and focal staining were found in 7/13 and 6/13 cases, respectively. Significant associations were observed with mucinous carcinoma subtype, invasion patterns, and lymph node metastasis. All KRAS-mutated cases showed negative BRAF staining. Complete absence of BRAF V600E IHC staining in CRC is strongly associated with KRAS mutation, suggesting IHC as an efficient tool to predict KRAS mutational status.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"232-244"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SARS-CoV-2 nucleocapsid detection using a recombinant phage display-isolated single-chain fragment variable. 利用重组噬菌体展示分离单链片段变量检测SARS-CoV-2核衣壳。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-03-24 DOI: 10.1080/15321819.2025.2483839

Abbasali Salarifar, Mohammad Javad Rasaee

{"title":"SARS-CoV-2 nucleocapsid detection using a recombinant phage display-isolated single-chain fragment variable.","authors":"Abbasali Salarifar, Mohammad Javad Rasaee","doi":"10.1080/15321819.2025.2483839","DOIUrl":"10.1080/15321819.2025.2483839","url":null,"abstract":"Background: Diagnosis is an important factor in controlling disease. Single-chain fragment variables (scFvs) can be used for diagnosis; however, due to their immobilization issues, their application has been limited. Herein, we isolated a SARS-CoV-2 nucleocapsid phosphoprotein (NP)-specific scFv and propose it as a diagnostic tool in the scFv-displaying phage format to overcome the immobilization issue.Method: Spleen from NP-immunized BALB/c mice was isolated, total RNA was extracted, and cDNA was synthesized. An scFv library was constructed, using the splicing by overlap extension (SOE) PCR technique, which was cloned into the pCANTAB5E phagemid. The phage library was panned against the NP antigen, and the output phages with the highest binding capability were screened for the most qualified scFv, which was later assessed in terms of sensitivity and specificity.Results: The scFv-displaying phage library was panned against the recombinant NP in three rounds and 40 randomly selected colonies from the third round's outputs were screened. Alongside several clones, clone #31 was chosen as the most qualified scFv, which later exhibited favorable sensitivity and specificity against NP in further ELISA-based experiments.Conclusions: Clone #31 could be utilized to develop diagnostic tools and therapeutics against SARS-CoV-2.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"369-387"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between autoimmunity and COPD: an investigation based on proteomic profiling and antinuclear antibody screening. 自身免疫与慢性阻塞性肺病的关系：基于蛋白质组学分析和抗核抗体筛选的研究。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-05-06 DOI: 10.1080/15321819.2025.2502430

Sonu Das, Supriya Adiody, Jinsu Varghese, Mathew John

{"title":"Relationship between autoimmunity and COPD: an investigation based on proteomic profiling and antinuclear antibody screening.","authors":"Sonu Das, Supriya Adiody, Jinsu Varghese, Mathew John","doi":"10.1080/15321819.2025.2502430","DOIUrl":"10.1080/15321819.2025.2502430","url":null,"abstract":"Chronic Obstructive Pulmonary Disease is a major global health concern with significant morbidity and mortality, characterized by heterogeneity influenced by inflammation, oxidative stress, and autoimmunity. This study investigated the role of autoimmunity in stable and exacerbated COPD phenotypes using proteomic and immunological analyses to elucidate molecular mechanisms and identify potential biomarkers. Plasma samples from COPD phenotypes and healthy controls were analyzed using label-free mass spectrometry to identify differentially expressed proteins. Functional annotation and pathway enrichment analysis highlighted proteins linked to autoimmunity, while immunological assays assessed anti-nuclear antibody prevalence and intensity using ANA ELISA and indirect immunofluorescence. The study identified differentially expressed proteins, namely, DNA repair protein XRCC2, phosphatidyl inositol glycan-specific phospholipase D, E3 ubiquitin protein ligase SHPRH, and Protocadherin-β, implicated in autoimmune pathways. Pathway enrichment analysis of these proteins highlighted the uPAR-mediated signaling, mTOR, PI3K/Akt, ARF6, and S1P signaling pathways, known for their roles in autoimmunity. Immunological assays revealed ANA positivity in 47% of stable COPD and 36% of exacerbated COPD, among which, 80% exhibited a speckled fluorescence pattern, often associated with anti-SSA and anti-SSB antibodies. The findings highlight the potential role of autoimmunity in COPD pathogenesis, suggesting phenotype-specific immune dysregulation, providing a basis for future biomarker and therapeutic research.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"339-352"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seroprevalence of H9N2 and H5 avian influenza in mixed-species poultry farms in Northern Benin. 贝宁北部混种家禽养殖场H9N2和H5禽流感的血清流行率

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-04-24 DOI: 10.1080/15321819.2025.2496480

Edmond Onidje, Oluwole Oyetunde Oni, Mireille Catherine Kadja, Modupe Beatrice Abraham, Vitus Burimuah, Amponsah Patrick Mensah, Derrick Adu Asare, Johnson Opoku Bannor, Benjamin Obukowho Emikpe

{"title":"Seroprevalence of H9N2 and H5 avian influenza in mixed-species poultry farms in Northern Benin.","authors":"Edmond Onidje, Oluwole Oyetunde Oni, Mireille Catherine Kadja, Modupe Beatrice Abraham, Vitus Burimuah, Amponsah Patrick Mensah, Derrick Adu Asare, Johnson Opoku Bannor, Benjamin Obukowho Emikpe","doi":"10.1080/15321819.2025.2496480","DOIUrl":"10.1080/15321819.2025.2496480","url":null,"abstract":"Avian influenza is one of the major threats to poultry and human health in northern Benin, while mixed-species farming systems increase the risks of viral transmission. The present study estimated the seroprevalence of avian influenza subtypes H9N2 and H5 in indigenous chickens and guinea fowls in the Atacora and Donga regions. A total of 300 birds including 191 indigenous chickens and 109 guinea fowls, from six districts were sampled through a cross-sectional survey using systematic random sampling. Hemagglutination inhibition assay was used to detect antibodies, revealing an overall H9N2 seroprevalence of 41%, with 17.5% of samples testing positive for H5. The seroprevalence of H9N2 was notably higher in guinea fowls (51.81% in Atacora and 52% in Donga) compared to chickens (34.95% in Atacora and 34.83% in Donga). H5 antibodies were found only in guinea fowls in Atacora (46.66%). The study also found that farms with both chickens and guinea fowls had a significantly higher odds ratio for H9N2 positivity (OR = 4.25, p < 0.001) compared to chicken-only farms. The results underscore the importance of mixed-species systems in the transmission of avian influenza, suggesting that targeted surveillance and biosecurity measures are essential for controlling the spread of these viruses.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"419-433"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical GATA3 expression in prostate adenocarcinoma: pitfalls. 免疫组化GATA3在前列腺癌中的表达：陷阱。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-04-27 DOI: 10.1080/15321819.2025.2499640

Sarra Ben Rejeb, Yasmine Chaabane, Kalthoum Dridi, Rym Bahloul, Hassen Khouni

{"title":"Immunohistochemical GATA3 expression in prostate adenocarcinoma: pitfalls.","authors":"Sarra Ben Rejeb, Yasmine Chaabane, Kalthoum Dridi, Rym Bahloul, Hassen Khouni","doi":"10.1080/15321819.2025.2499640","DOIUrl":"10.1080/15321819.2025.2499640","url":null,"abstract":"Background: Recent studies have reported aberrant GATA3 immunohistochemical expression in high-grade prostate carcinoma, raising concerns about its specificity. This study aimed to evaluate GATA3 immunohistochemical expression in prostate adenocarcinoma.Methods: We retrospectively collected cases of primary prostate adenocarcinoma diagnosed in our pathology department(2019-2024). Clinical and pathological data, including Gleason and ISUP grades, were collected.An automated immunohistochemical analysis was performed using the anti-GATA3 antibody(Leica, L50-823). Only nuclear staining was considered positive.Results: Fifty-eight cases have been included in the study with a mean age of 67.8 years-old. GATA3 nuclear expression was observed in 6.9% (4/58) of cases, all classified as Gleason 7 (4 + 3) and ISUP grade 3. Only the Gleason 4 or 5 components showed staining, while Gleason 3 components were negative. Additionally, benign prostate glands and seminal vesicles showed rare GATA3 expression (4/58 and 1/58 cases, respectively).Conclusion: While GATA3 is commonly used as a marker for urothelial carcinoma, our findings indicate that it can also be expressed in prostate adenocarcinoma, particularly in higher Gleason grade components, which may pose a diagnostic pitfall. Further studies are needed to determine the clinical significance of GATA3 expression in prostate adenocarcinoma and its potential impact on tumor behavior and prognosis.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"331-338"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance of a point of care allergy test compared to an established laboratory test for specific IgE in Japanese patients with allergic rhinitis. 日本变应性鼻炎患者特异性IgE的护理点过敏测试与既定实验室测试的比较

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-04-16 DOI: 10.1080/15321819.2025.2490541

Tomohiro Ono, Tadamitsu Morimoto, Tomoya Hanakita, Kizuki Watanabe, Chie Iwamura, Natsuko Asakura

{"title":"Performance of a point of care allergy test compared to an established laboratory test for specific IgE in Japanese patients with allergic rhinitis.","authors":"Tomohiro Ono, Tadamitsu Morimoto, Tomoya Hanakita, Kizuki Watanabe, Chie Iwamura, Natsuko Asakura","doi":"10.1080/15321819.2025.2490541","DOIUrl":"10.1080/15321819.2025.2490541","url":null,"abstract":"Background: Pollinosis is highly prevalent. Reliable specific IgE tests are needed to identify the causative aeroallergens. This study aims to evaluate the performance of DropScreenTM (DS), a point of care test, by comparing it with that of ViewAllergy39TM, a laboratory test (VA).Methods: Sera from 70 Japanese outpatients with pollinosis (median age: 29.5 years; 35% were men) were used. Qualitative analysis and concordance rate assessment were performed. For allergens with discrepant results between DS and VA, inhibition tests were carried out on relevant samples and allergen component analysis for specific allergens was conducted.Results: The average positivity rates for the 17 allergens included in the study were 20.8% and 26.6% for DS and VA, respectively. DS positive rate for Japanese cypress was 10-fold lower than that of VA. Clear discrepancies in detection rates between VA and DS were also shown for Japanese cedar, Moth, and A. fumigatus. Inhibition tests on discrepant sera confirmed that VA could accurately measure specific IgE toward these allergens.Conclusion: DS and VA were not equally effective in reliably detecting specific IgE toward Japanese cypress. This study highlights the importance of understanding the impact of testing performance differences between assays on diagnostic workup and subsequent therapy selection.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"388-402"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High prevalence of antinuclear antibodies in hepatitis C related hepatocellular carcinoma. 丙型肝炎相关肝细胞癌中抗核抗体的高流行率。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-03-17 DOI: 10.1080/15321819.2025.2480368

Mohamed El-Far, Ahmed S Mustafa, AbdelfattahM Attallah, Mohamed A Abdelrazek

{"title":"High prevalence of antinuclear antibodies in hepatitis C related hepatocellular carcinoma.","authors":"Mohamed El-Far, Ahmed S Mustafa, AbdelfattahM Attallah, Mohamed A Abdelrazek","doi":"10.1080/15321819.2025.2480368","DOIUrl":"10.1080/15321819.2025.2480368","url":null,"abstract":"In chronic hepatitis C (CHC), the virus may induce autoimmune responses via autoantibodies production, including antinuclear antibodies (ANA). Former studies reported great ANA predisposition in CHC and these ANAs may be related to worse prognosis including hepatocellular carcinoma (HCC). We aimed to evaluate the association between ANA incidence and CHC-related HCC development and to evaluate these molecules effect on HCC severity including tumor size and advanced stages. Results revealed that ANA seropositivity was associated with disease severity. HCC patients (54%, OR = 9.7) were associated with ANA positivity more than liver cirrhosis (24.5%) and fibrosis (10.8%). ANA positivity was significantly high in patients with severe tumor features including macrovascular invasion (61.9%; OR = 8.1), large size (68.2%; OR = 2.4), Child C (83.3%; OR = 8.1), BCLC end stage (83.3%; OR = 8.6) and advanced CLIP stage (80.9%; OR = 7.9). ANA positivity were significantly (p < 0.05) correlated with some estimated liver fibrosis related biomarkers including EMA (r = 0.206), fibronectin (r = 0.273), cytokeratin-1 (r = 0.365) and collagen III (r = 0.324). In conclusion, our observation of increased ANA+ serum samples among CHC-related HCC might suggest the oncogenic role of ANA in such patients. Also, clinicians need to appreciate value of ANA testing among HCC patients as these molecules were associated with tumor severity and worse outcomes.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"218-231"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implication of TRPM8, CD47, and CDK4 expressions in hepatocellular carcinoma progression. TRPM8、CD47和CDK4表达在肝癌进展中的意义

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-02-16 DOI: 10.1080/15321819.2025.2464718

Aiat Shaban Hemida, Mona Saeed Tantawy

{"title":"Implication of TRPM8, CD47, and CDK4 expressions in hepatocellular carcinoma progression.","authors":"Aiat Shaban Hemida, Mona Saeed Tantawy","doi":"10.1080/15321819.2025.2464718","DOIUrl":"10.1080/15321819.2025.2464718","url":null,"abstract":"Participation of TRPM8 in hepatocellular carcinoma (HCC) development was not precisely declared. CD47 mediates immune escape and macrophage phagocytosis of tumors. CDK4 mediates oncogenesis. Synergistic implications of TRPM8, CD47, and CDK4 in HCC were not declared. This research aims to demonstrate the expressions of TRPM8, CD47and CDK4 in HCC and to declare correlations and significance. Paraffin blocks from 101 hCC and 82 adjacent non-tumorous liver were immunostained using TRPM8, CD47 and CDK4 antibodies. HCC showed highly significant increased TRPM8, CD47, and CDK4 expressions than control liver tissue (p < 0.001) for all. TRPM8, CD47, and CDK4 were significantly associated with poor prognostic criteria as high tumor grade, advanced stage, microvascular invasion, and necrosis. There was a significant association between cirrhotic and non-cirrhotic adjacent liver regarding positive TRPM8 (p < 0.02) and high CDK4 expressions (p < 0.045). There were significant direct relationships between each immunohistochemical antibody and the other two. Prolonged overall survival was significantly associated with low CDK4 (p = 0.019). In conclusion, TRPM8, CD47, and CDK4 may regulate synergistic functions in HCC oncogenesis and accomplish unfavorable prognostic significance. TRPM8 and CDK4 might share in development of HCC from cirrhosis. TRPM8, CD47, and CDK4 could be therapeutic targets in HCC.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"245-261"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lymphangioinvasion detection using the monoclonal antibody D2-40 (Podoplanin)as a clinical predictor of axillary lymph node metastasis in breast cancer patients. 使用单克隆抗体D2-40 （Podoplanin）检测淋巴管侵袭作为乳腺癌患者腋窝淋巴结转移的临床预测指标。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-02-23 DOI: 10.1080/15321819.2025.2470434

Teresa Cistina Ferreira Gutman, Angela de Salles Rezende, Dyego Mondego Moraes, Consuelo Lozoya Lopez, Licínio Esmeraldo da Silva, Rafaela Elvira Rozza-de-Menezes, João Paulo Lima Daher, André Vallejo da Silva, Fabiana Resende Rodrigues, Vânia Gloria Silami Lopes

{"title":"Lymphangioinvasion detection using the monoclonal antibody D2-40 (Podoplanin)as a clinical predictor of axillary lymph node metastasis in breast cancer patients.","authors":"Teresa Cistina Ferreira Gutman, Angela de Salles Rezende, Dyego Mondego Moraes, Consuelo Lozoya Lopez, Licínio Esmeraldo da Silva, Rafaela Elvira Rozza-de-Menezes, João Paulo Lima Daher, André Vallejo da Silva, Fabiana Resende Rodrigues, Vânia Gloria Silami Lopes","doi":"10.1080/15321819.2025.2470434","DOIUrl":"10.1080/15321819.2025.2470434","url":null,"abstract":"Breast is a major global health issue and the most common cancer in women. Identifying vascular invasion is challenging due to the need to distinguish true invasion from artifacts. This study explored lymphatic embolism in invasive breast carcinoma using the monoclonal antibody D2-40 as a prognostic indicator. A total of 100 patients with invasive breast carcinoma from 2009 to 2011 were included in the study. Tissue microarray technique (TMA) was used on patient tissue, constructing three paraffin blocks from each participant's histological data. Immunohistochemistry with D2-40 and CD34 antibodies was performed to identify lymphatic and blood emboli, respectively, and results were compared with previous findings. A prior report using hematoxylin-eosin staining found fewer patients with lymphatic emboli (34) compared to our study (56) using D2-40. Lymphatic emboli correlated with axillary metastases, with an odds ratio (OR) of 3.50, a 95% confidence interval (CI) of 1.92-5.08, and a p-value of 0.001, whereas hematoxylin-eosin alone showed OR = 1.42, 95% CI = 0.40-3.47, and p-value = 0.23. TMA with D2-40 staining detected more lymphatic emboli than hematoxylin-eosin staining alone. Higher embolic expression rates are linked to increased tumor aggressiveness, worse prognosis and shorter overall survival.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"274-288"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic significance of B-Catenin and E-Cadherin expression in gastric carcinoma. 胃癌中B-Catenin和E-Cadherin表达的预后意义。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-05-14 DOI: 10.1080/15321819.2025.2505033

Sarra Ben Rejeb, Abir Labadi, Marwa Lakhal, Khadija Bellil, Adnen Chouchen

{"title":"Prognostic significance of B-Catenin and E-Cadherin expression in gastric carcinoma.","authors":"Sarra Ben Rejeb, Abir Labadi, Marwa Lakhal, Khadija Bellil, Adnen Chouchen","doi":"10.1080/15321819.2025.2505033","DOIUrl":"10.1080/15321819.2025.2505033","url":null,"abstract":"Introduction: Gastric cancer (GC) is a leading cause of cancer-related mortality worldwide. Dysregulation of molecular pathways, including β-Catenin-mediated Wnt signaling, epithelial-to-mesenchymal transition (EMT), and E-Cadherin-modulated cell adhesion, plays critical roles in gastric carcinogenesis. This study assesses the expression patterns of β-Catenin and E-Cadherin in GC and explores their prognostic significance.Methods: This retrospective, multi-center study analyzed GC cases diagnosed between 2009 and 2019 at the pathology departments of Security Forces and Rabta Hospitals. Tissue microarray (TMA) paraffin blocks from 48 GC cases were immunohistochemically stained using antibodies for β-Catenin (Leica, 17C2) and E-Cadherin (Leica, 36B5). β-Catenin expression was scored as membranous, cytoplasmic, or nuclear, with overexpression defined as ≥ 50% positive cells. E-Cadherin staining was categorized from absent (score 0) to marked membranous staining (score 3), with scores 0-2 considered aberrant. Statistical analysis was performed using SPSS version 23.Results: Of the 48 cases, β-Catenin overexpression was observed in 50% of cases, significantly associated with tumor differentiation (p = 0.033), age > 60 years (p = 0.042), and male sex (p = 0.028). Aberrant E-Cadherin expression was found in 65% of cases, linked to poorly cohesive and diffuse subtypes (p = 0.053), poor differentiation (p = 0.042), and recurrence (p = 0.043), with a trend toward reduced survival (p = 0.056).Conclusion: β-Catenin overexpression and aberrant E-Cadherin expression are frequent in GC, reflecting their roles in tumor progression via Wnt signaling and EMT. These findings highlight their potential as prognostic biomarkers and therapeutic targets, particularly for Wnt pathway-directed therapies in personalized GC management.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"317-330"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0