Journal of immunoassay & immunochemistry最新文献_第3页

Seroprevalence and associated risk factors of Newcastle disease in guinea fowls in the Upper East region of Ghana. 加纳上东部地区珍珠鸡新城疫血清流行率及相关危险因素

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-06-12 DOI: 10.1080/15321819.2025.2518206

Albert Agyapong Tweneboah, Sherry Ama Mawuko Johnson, Patrick Mensah Amponsah, Derrick Adu Asare, Benjamin Obukowho Emikpe

{"title":"Seroprevalence and associated risk factors of Newcastle disease in guinea fowls in the Upper East region of Ghana.","authors":"Albert Agyapong Tweneboah, Sherry Ama Mawuko Johnson, Patrick Mensah Amponsah, Derrick Adu Asare, Benjamin Obukowho Emikpe","doi":"10.1080/15321819.2025.2518206","DOIUrl":"10.1080/15321819.2025.2518206","url":null,"abstract":"Newcastle Disease (ND) is a major constraint to poultry production in sub-Saharan Africa. However, information on Newcastle disease in guinea fowls is scant in Ghana. This study seeks to detect circulating antibodies against ND and the risk factors for occurrence in guinea fowls in the Upper East region of Ghana. Sera was obtained from guinea fowls from households, live bird markets, and slaughter points in different locations in the Upper East region. The sera were evaluated for antibodies against Newcastle disease using the Haemagglutination Inhibition (HI) test. Questionnaire was administered to the farmers to assess risk factors such as vaccination status, management system, and contact with wild birds. Almost half, 213/431 (49.4%, 95% CI = 44.6-54.2) of the guinea fowls were seropositive. The seroprevalence was 46.2% in live bird markets, 36.63% in households, and 52.7% at slaughter points. A seroprevalence of 49.7% was observed in adults and 45.7% growers. There was a 53.4% prevalence in males and 43.9% in females. Antibodies to ND were found circulating in guinea fowls in the Upper East region. We recommend that guinea fowls be made an important component element in the ND surveillance for an effective monitoring and control in the region.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"403-418"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CXCR4 expression in immunohistochemistry of gastrointestinal neuroendocrine neoplasms: a meta-analysis. CXCR4在胃肠道神经内分泌肿瘤免疫组织化学中的表达：一项meta分析。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-03-24 DOI: 10.1080/15321819.2025.2482642

Changlong Ruan, Jiajia Zhang, Yingying Liao, Yujie Hang, Yuan Dong, Hanfeng Shi, Xiaoya Wang

{"title":"CXCR4 expression in immunohistochemistry of gastrointestinal neuroendocrine neoplasms: a meta-analysis.","authors":"Changlong Ruan, Jiajia Zhang, Yingying Liao, Yujie Hang, Yuan Dong, Hanfeng Shi, Xiaoya Wang","doi":"10.1080/15321819.2025.2482642","DOIUrl":"10.1080/15321819.2025.2482642","url":null,"abstract":"Objective: C-X-C chemokine receptor type 4 (CXCR4) is significantly associated with the development of various malignant tumors. This meta-analysis aims to investigate the expression of CXCR4 in the immunohistochemistry of gastrointestinal neuroendocrine neoplasms (GI-NENs).Methods: A comprehensive literature search regarding gastrointestinal neuroendocrine neoplasms and CXCR4 was conducted using PubMed,Web of Science, and the Cochrane Library, with a cut off date of June 30, 2024.Two researchers independently screened the literature according to inclusion and exclusion criteria and assessed study quality using the Newcastle-OttawaScale. Meta-analysis was performed using Stata version 17.0. The pooled positive rate was employed to evaluate the expression of CXCR4 in the immunohistochemistry of GI-NENs.Results: A total of eight studies involving 501 patients were included in this research. The immunohistochemical analysis revealed positive CXCR4 expression in 174 patients. The meta-analysis calculated and summarized the combined positive rate (R: 0.41; 95% CI = 0.21-0.60, p = 0.00), indicating that the differences were statistically significant.Conclusion: CXCR4 is highly expressed in the immunohistochemistry of GI-NENs, which may provide some evidence for the therapeutic application of CXCR4 antagonists in treating neuroendocrine neoplasms.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"209-217"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of honey-ginger mouthwash on oral mucositis in patients undergoing chemotherapy. 蜂蜜姜漱口水对化疗患者口腔黏膜炎的影响。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-03-25 DOI: 10.1080/15321819.2025.2484645

Fatemeh Sadat Razavi, Hania Zokaee, Mojtaba Sehat, Mohsen Taghizadeh, Hossein Motedayyen, Elaheh Ghasemzadeh Hoseini

{"title":"Effect of honey-ginger mouthwash on oral mucositis in patients undergoing chemotherapy.","authors":"Fatemeh Sadat Razavi, Hania Zokaee, Mojtaba Sehat, Mohsen Taghizadeh, Hossein Motedayyen, Elaheh Ghasemzadeh Hoseini","doi":"10.1080/15321819.2025.2484645","DOIUrl":"10.1080/15321819.2025.2484645","url":null,"abstract":"Background: Oral mucositis is considered as one of the most prevalent complications of chemotherapy or radiation therapy in cancerous tumors, which can interrupt the patient's treatment and nutrition. This study therefore aimed to evaluate the efficacy of ginger-honey mouthwash on the prevention of chemotherapy-induced oral mucositis in patients suffering from various cancers.Materials and methods: In this randomized clinical trial study, 70 patients receiving chemotherapy were divided into case and control groups. The former group (n = 34) received natural honey-ginger mouthwash and the latter (n = 36) used normal saline for 14 days. The presence and severity of oral mucositis, pain intensity, and other related characteristics were evaluated based on a two-part questionnaire (demographic and clinical information) and a checklist prepared from the protocols of the World Health Organization in each group.Results: During a 14-day intervention, patients received a 7-day intervention with ginger-honey mouthwash revealed a significant reduction in the mean severity of oral mucositis compared to the control group (p = 0.03). However, a 14-day intervention with ginger-honey mouthwash indicated no significant impact on the mean severity of oral mucositis (p = 0.6). In addition, no significant difference was observed in pain intensity between case and control groups during these 14 days.Conclusions: This study suggests that a seven-day intervention with ginger-honey mouthwash has a beneficial effect on reducing the severity of mucositis in patients under chemotherapy, unlike a 14-day intervention. The honey-ginger mouthwash fails to have a significant effect on the pain intensity due to mucositis in patients undergoing chemotherapy.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"303-315"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of IL2-330 (rs2069762) gene polymorphism in Egyptian patients with lung cancer: a case-control study. IL2-330 （rs2069762）基因多态性在埃及肺癌患者中的研究：一项病例对照研究

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-03-18 DOI: 10.1080/15321819.2025.2477605

Esraa Tawfik Allam, Mohamed S El Senbawy, Hanaa M Elgazzar, Inas Moaz, Sara Ahmed Saied, Hind S AboShabaan, Shimaa Mohammed Hamad, Samah Ahmed Elbeltagy, Omnia S Nabih

{"title":"Study of IL2-330 (rs2069762) gene polymorphism in Egyptian patients with lung cancer: a case-control study.","authors":"Esraa Tawfik Allam, Mohamed S El Senbawy, Hanaa M Elgazzar, Inas Moaz, Sara Ahmed Saied, Hind S AboShabaan, Shimaa Mohammed Hamad, Samah Ahmed Elbeltagy, Omnia S Nabih","doi":"10.1080/15321819.2025.2477605","DOIUrl":"10.1080/15321819.2025.2477605","url":null,"abstract":"Background: IL2 is one of the key cytokines essential for regulating the immune system and the inflammation-related carcinogenesis process. Few studies have examined the relationship between lung cancer and the IL2-330 (rs2069762) gene polymorphism, despite several studies demonstrating that it is linked to numerous cancer types.Objective: Our study aimed to investigate the association between IL2-330 (rs2069762) polymorphism and lung cancer risk and explore the role of IL2-330 polymorphism in survival outcomes (OS and PFS).Patients and methods: The study was conducted from October 2023 to November 2024, including 50 randomly selected patients diagnosed with lung cancer and 50 subjects matched for age and gender were used as controls in this case-control study. The IL2-330 (rs2069762) gene polymorphism was assessed using real-time PCR.Results: We found that the AC genotype was associated with a notably lower risk of lung cancer in comparison to the AA genotype (95% CI: 0.06-0.61, p = 0.01). Conversely, the CC genotype showed no significant association with lung cancer risk when compared to the reference genotype. The comprehensive comparison of survival distributions among the AA, AC, and CC genotypes through the Log-Rank (Mantel-Cox) test indicated no statistically significant difference.Conclusions: According to our research, The AC genotype of IL2-330 (rs2069762) is associated with a significantly higher survival rate and lower risk of lung cancer. Further future studies are needed to confirm these findings.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"289-302"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BRAF V600E protein detection by immunohistochemistry in metastatic colorectal cancer tissue: association with tumor characteristics and KRAS mutation. 转移性结直肠癌组织中BRAF V600E蛋白的免疫组化检测：与肿瘤特征和KRAS突变的关系

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-03-26 DOI: 10.1080/15321819.2025.2484408

Sarra Ben Rejeb, Safia Sakly, Rym Bahloul, Adnen Chouchen

{"title":"BRAF V600E protein detection by immunohistochemistry in metastatic colorectal cancer tissue: association with tumor characteristics and KRAS mutation.","authors":"Sarra Ben Rejeb, Safia Sakly, Rym Bahloul, Adnen Chouchen","doi":"10.1080/15321819.2025.2484408","DOIUrl":"10.1080/15321819.2025.2484408","url":null,"abstract":"Immunohistochemistry (IHC) using the BRAF V600E antibody is a sensitive and specific method for detecting BRAF V600E mutations in colorectal cancer (CRC). Given that BRAF and KRAS mutations are mutually exclusive, this study aimed to assess the expression of BRAF V600E in CRC according to KRAS mutation status. Automated IHC analysis was performed on tissue samples from metastatic CRC patients diagnosed between 2012 and 2022 using the anti-BRAF V600E antibody (GenomeME, IHC600). Positive and negative control tissues were used for validation. Cytoplasmic staining was considered positive, with intensity classified as weak, moderate, or strong. The percentage of positive tumor cells was recorded semi-quantitatively. Thirty-five cases were included.The mean age of patients was 60 years (±12.41), with a male-to-female ratio of 2.9. KRAS mutations were present in 48% of cases. BRAF V600E cytoplasmic staining was observed in 37.1%, with a mean percentage of positive cells of 50% (range: 5%-100%). Diffuse and focal staining were found in 7/13 and 6/13 cases, respectively. Significant associations were observed with mucinous carcinoma subtype, invasion patterns, and lymph node metastasis. All KRAS-mutated cases showed negative BRAF staining. Complete absence of BRAF V600E IHC staining in CRC is strongly associated with KRAS mutation, suggesting IHC as an efficient tool to predict KRAS mutational status.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"232-244"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SARS-CoV-2 nucleocapsid detection using a recombinant phage display-isolated single-chain fragment variable. 利用重组噬菌体展示分离单链片段变量检测SARS-CoV-2核衣壳。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-03-24 DOI: 10.1080/15321819.2025.2483839

Abbasali Salarifar, Mohammad Javad Rasaee

{"title":"SARS-CoV-2 nucleocapsid detection using a recombinant phage display-isolated single-chain fragment variable.","authors":"Abbasali Salarifar, Mohammad Javad Rasaee","doi":"10.1080/15321819.2025.2483839","DOIUrl":"10.1080/15321819.2025.2483839","url":null,"abstract":"Background: Diagnosis is an important factor in controlling disease. Single-chain fragment variables (scFvs) can be used for diagnosis; however, due to their immobilization issues, their application has been limited. Herein, we isolated a SARS-CoV-2 nucleocapsid phosphoprotein (NP)-specific scFv and propose it as a diagnostic tool in the scFv-displaying phage format to overcome the immobilization issue.Method: Spleen from NP-immunized BALB/c mice was isolated, total RNA was extracted, and cDNA was synthesized. An scFv library was constructed, using the splicing by overlap extension (SOE) PCR technique, which was cloned into the pCANTAB5E phagemid. The phage library was panned against the NP antigen, and the output phages with the highest binding capability were screened for the most qualified scFv, which was later assessed in terms of sensitivity and specificity.Results: The scFv-displaying phage library was panned against the recombinant NP in three rounds and 40 randomly selected colonies from the third round's outputs were screened. Alongside several clones, clone #31 was chosen as the most qualified scFv, which later exhibited favorable sensitivity and specificity against NP in further ELISA-based experiments.Conclusions: Clone #31 could be utilized to develop diagnostic tools and therapeutics against SARS-CoV-2.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"369-387"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between autoimmunity and COPD: an investigation based on proteomic profiling and antinuclear antibody screening. 自身免疫与慢性阻塞性肺病的关系：基于蛋白质组学分析和抗核抗体筛选的研究。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-05-06 DOI: 10.1080/15321819.2025.2502430

Sonu Das, Supriya Adiody, Jinsu Varghese, Mathew John

{"title":"Relationship between autoimmunity and COPD: an investigation based on proteomic profiling and antinuclear antibody screening.","authors":"Sonu Das, Supriya Adiody, Jinsu Varghese, Mathew John","doi":"10.1080/15321819.2025.2502430","DOIUrl":"10.1080/15321819.2025.2502430","url":null,"abstract":"Chronic Obstructive Pulmonary Disease is a major global health concern with significant morbidity and mortality, characterized by heterogeneity influenced by inflammation, oxidative stress, and autoimmunity. This study investigated the role of autoimmunity in stable and exacerbated COPD phenotypes using proteomic and immunological analyses to elucidate molecular mechanisms and identify potential biomarkers. Plasma samples from COPD phenotypes and healthy controls were analyzed using label-free mass spectrometry to identify differentially expressed proteins. Functional annotation and pathway enrichment analysis highlighted proteins linked to autoimmunity, while immunological assays assessed anti-nuclear antibody prevalence and intensity using ANA ELISA and indirect immunofluorescence. The study identified differentially expressed proteins, namely, DNA repair protein XRCC2, phosphatidyl inositol glycan-specific phospholipase D, E3 ubiquitin protein ligase SHPRH, and Protocadherin-β, implicated in autoimmune pathways. Pathway enrichment analysis of these proteins highlighted the uPAR-mediated signaling, mTOR, PI3K/Akt, ARF6, and S1P signaling pathways, known for their roles in autoimmunity. Immunological assays revealed ANA positivity in 47% of stable COPD and 36% of exacerbated COPD, among which, 80% exhibited a speckled fluorescence pattern, often associated with anti-SSA and anti-SSB antibodies. The findings highlight the potential role of autoimmunity in COPD pathogenesis, suggesting phenotype-specific immune dysregulation, providing a basis for future biomarker and therapeutic research.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"339-352"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seroprevalence of H9N2 and H5 avian influenza in mixed-species poultry farms in Northern Benin. 贝宁北部混种家禽养殖场H9N2和H5禽流感的血清流行率

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-04-24 DOI: 10.1080/15321819.2025.2496480

Edmond Onidje, Oluwole Oyetunde Oni, Mireille Catherine Kadja, Modupe Beatrice Abraham, Vitus Burimuah, Amponsah Patrick Mensah, Derrick Adu Asare, Johnson Opoku Bannor, Benjamin Obukowho Emikpe

{"title":"Seroprevalence of H9N2 and H5 avian influenza in mixed-species poultry farms in Northern Benin.","authors":"Edmond Onidje, Oluwole Oyetunde Oni, Mireille Catherine Kadja, Modupe Beatrice Abraham, Vitus Burimuah, Amponsah Patrick Mensah, Derrick Adu Asare, Johnson Opoku Bannor, Benjamin Obukowho Emikpe","doi":"10.1080/15321819.2025.2496480","DOIUrl":"10.1080/15321819.2025.2496480","url":null,"abstract":"Avian influenza is one of the major threats to poultry and human health in northern Benin, while mixed-species farming systems increase the risks of viral transmission. The present study estimated the seroprevalence of avian influenza subtypes H9N2 and H5 in indigenous chickens and guinea fowls in the Atacora and Donga regions. A total of 300 birds including 191 indigenous chickens and 109 guinea fowls, from six districts were sampled through a cross-sectional survey using systematic random sampling. Hemagglutination inhibition assay was used to detect antibodies, revealing an overall H9N2 seroprevalence of 41%, with 17.5% of samples testing positive for H5. The seroprevalence of H9N2 was notably higher in guinea fowls (51.81% in Atacora and 52% in Donga) compared to chickens (34.95% in Atacora and 34.83% in Donga). H5 antibodies were found only in guinea fowls in Atacora (46.66%). The study also found that farms with both chickens and guinea fowls had a significantly higher odds ratio for H9N2 positivity (OR = 4.25, p < 0.001) compared to chicken-only farms. The results underscore the importance of mixed-species systems in the transmission of avian influenza, suggesting that targeted surveillance and biosecurity measures are essential for controlling the spread of these viruses.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"419-433"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical GATA3 expression in prostate adenocarcinoma: pitfalls. 免疫组化GATA3在前列腺癌中的表达：陷阱。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-04-27 DOI: 10.1080/15321819.2025.2499640

Sarra Ben Rejeb, Yasmine Chaabane, Kalthoum Dridi, Rym Bahloul, Hassen Khouni

{"title":"Immunohistochemical GATA3 expression in prostate adenocarcinoma: pitfalls.","authors":"Sarra Ben Rejeb, Yasmine Chaabane, Kalthoum Dridi, Rym Bahloul, Hassen Khouni","doi":"10.1080/15321819.2025.2499640","DOIUrl":"10.1080/15321819.2025.2499640","url":null,"abstract":"Background: Recent studies have reported aberrant GATA3 immunohistochemical expression in high-grade prostate carcinoma, raising concerns about its specificity. This study aimed to evaluate GATA3 immunohistochemical expression in prostate adenocarcinoma.Methods: We retrospectively collected cases of primary prostate adenocarcinoma diagnosed in our pathology department(2019-2024). Clinical and pathological data, including Gleason and ISUP grades, were collected.An automated immunohistochemical analysis was performed using the anti-GATA3 antibody(Leica, L50-823). Only nuclear staining was considered positive.Results: Fifty-eight cases have been included in the study with a mean age of 67.8 years-old. GATA3 nuclear expression was observed in 6.9% (4/58) of cases, all classified as Gleason 7 (4 + 3) and ISUP grade 3. Only the Gleason 4 or 5 components showed staining, while Gleason 3 components were negative. Additionally, benign prostate glands and seminal vesicles showed rare GATA3 expression (4/58 and 1/58 cases, respectively).Conclusion: While GATA3 is commonly used as a marker for urothelial carcinoma, our findings indicate that it can also be expressed in prostate adenocarcinoma, particularly in higher Gleason grade components, which may pose a diagnostic pitfall. Further studies are needed to determine the clinical significance of GATA3 expression in prostate adenocarcinoma and its potential impact on tumor behavior and prognosis.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"331-338"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance of a point of care allergy test compared to an established laboratory test for specific IgE in Japanese patients with allergic rhinitis. 日本变应性鼻炎患者特异性IgE的护理点过敏测试与既定实验室测试的比较

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-04-16 DOI: 10.1080/15321819.2025.2490541

Tomohiro Ono, Tadamitsu Morimoto, Tomoya Hanakita, Kizuki Watanabe, Chie Iwamura, Natsuko Asakura

{"title":"Performance of a point of care allergy test compared to an established laboratory test for specific IgE in Japanese patients with allergic rhinitis.","authors":"Tomohiro Ono, Tadamitsu Morimoto, Tomoya Hanakita, Kizuki Watanabe, Chie Iwamura, Natsuko Asakura","doi":"10.1080/15321819.2025.2490541","DOIUrl":"10.1080/15321819.2025.2490541","url":null,"abstract":"Background: Pollinosis is highly prevalent. Reliable specific IgE tests are needed to identify the causative aeroallergens. This study aims to evaluate the performance of DropScreenTM (DS), a point of care test, by comparing it with that of ViewAllergy39TM, a laboratory test (VA).Methods: Sera from 70 Japanese outpatients with pollinosis (median age: 29.5 years; 35% were men) were used. Qualitative analysis and concordance rate assessment were performed. For allergens with discrepant results between DS and VA, inhibition tests were carried out on relevant samples and allergen component analysis for specific allergens was conducted.Results: The average positivity rates for the 17 allergens included in the study were 20.8% and 26.6% for DS and VA, respectively. DS positive rate for Japanese cypress was 10-fold lower than that of VA. Clear discrepancies in detection rates between VA and DS were also shown for Japanese cedar, Moth, and A. fumigatus. Inhibition tests on discrepant sera confirmed that VA could accurately measure specific IgE toward these allergens.Conclusion: DS and VA were not equally effective in reliably detecting specific IgE toward Japanese cypress. This study highlights the importance of understanding the impact of testing performance differences between assays on diagnostic workup and subsequent therapy selection.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"388-402"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0