Journal of immunoassay & immunochemistry最新文献_第4页

Costus speciosus extract: a natural immunomodulator for treatment of psoriasis in BALB/c mice by NF-κB pathway. 木香提取物：经NF-κB通路治疗BALB/c小鼠银屑病的天然免疫调节剂

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-05-02 DOI: 10.1080/15321819.2025.2498439

Aditi Vyas, Muqtada Shaikh, Radhika Raheja, Gaurav Doshi

{"title":"Costus speciosus extract: a natural immunomodulator for treatment of psoriasis in BALB/c mice by NF-κB pathway.","authors":"Aditi Vyas, Muqtada Shaikh, Radhika Raheja, Gaurav Doshi","doi":"10.1080/15321819.2025.2498439","DOIUrl":"10.1080/15321819.2025.2498439","url":null,"abstract":"Psoriasis is a chronic inflammatory skin condition. Ayurveda, Traditional Chinese therapy, and Unani offer a potential approach to treating psoriasis. This study explores the immunomodulatory effects of Costus speciosus petroleum ether extract (CSPE) on psoriasis-like symptoms in Balb/c mice. GC-MS analysis was done to detect bioactive phytoconstituents in the extract. Mice were treated with Imiquimod (IMQ) to induce psoriasis-like symptoms. Measurements of back skin thickness, skin length, mass, and body weight were used in the study, with an assessment of the Psoriasis Area and Severity Index (PASI). The treatment group received doses of 110 mg/kg, 220 mg/kg, and 440 mg/kg of extracts. Nuclear Factor kappa-B (NF-kB), tumor necrosis factor-alpha (TNF-α), and interleukin-17 (IL-17) were estimated. Presence of diosgenin, tigogenin, aglycone of diosgenin, santamarine and reynosin in the extract was confirmed by GC-MS analysis. At 440 mg/kg, a significant effect was observed in the IMQ model. The extract significantly reduced levels of NF-kB, TNF-α, and IL-17. The research findings indicate that CSPE is a promising candidate for psoriasis treatment.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"557-577"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-chain variable fragment antibodies targeting the antileukemia agent harringtonine for enzyme-linked immunosorbent assay development. 单链可变片段抗体靶向抗白血病药物哈林丁碱酶联免疫吸附试验的发展。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-07-09 DOI: 10.1080/15321819.2025.2526208

Seiichi Sakamoto, Shohei Komatsu, Ai Moriyasu, Gorawit Yusakul, Waraporn Putalun, Poomraphie Nuntawong, Hiroyuki Tanaka, Satoshi Morimoto

{"title":"Single-chain variable fragment antibodies targeting the antileukemia agent harringtonine for enzyme-linked immunosorbent assay development.","authors":"Seiichi Sakamoto, Shohei Komatsu, Ai Moriyasu, Gorawit Yusakul, Waraporn Putalun, Poomraphie Nuntawong, Hiroyuki Tanaka, Satoshi Morimoto","doi":"10.1080/15321819.2025.2526208","DOIUrl":"10.1080/15321819.2025.2526208","url":null,"abstract":"In this study, we generated a single-chain variable fragment (scFv) specific to a potent antileukemic substance, harringtonine (HT) (HT-scFv) that can be used in enzyme-linked immunosorbent assay (ELISA) for the quantitative analysis of HT in the plant genus Cephalotaxus. The variable heavy (VH) and light chain (VL) genes were directly cloned from the cDNA of the hybridoma cell line 1D2, which secretes monoclonal antibody against HT (MAb 1D2). These genes were then assembled with a flexible peptide linker, specifically (Gly4Ser)3, through splicing by overlap extension PCR. The resulting HT-scFv gene was expressed in Escherichia coli. The denatured HT-scFv, produced as inclusion bodies, was solubilized and refolded using a dilution method to restore its functionality as an antibody. Characterization of the HT-scFv demonstrated its high specificity for HT. Additionally, its remarkable properties facilitated the development of an ELISA for HT detection, achieving a limit of detection (LOD) of 12.2 ng/mL. Furthermore, validation analyses showed that the ELISA using HT-scFv exhibited good accuracy and reliability for the quantitative assessment of HT in C. harringtonia \"Fastigiata.\" This study highlights the potential of HT-scFv as a valuable tool for ELISA in the quantitative analysis of HT.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"519-534"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the impact of interferon-ß and rituximab on NLRP3 and AIM2 expression and IL-1β levels in patients with multiple sclerosis. 评估干扰素β和利妥昔单抗对多发性硬化症患者NLRP3和AIM2表达及IL-1β水平的影响。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-07-17 DOI: 10.1080/15321819.2025.2534453

Sina Molavizade, Fereshteh Ashtari, Fateme Dehghani, Zahra Karimi, Mohammad Mahjoubi, Nasrin Zare

{"title":"Assessment of the impact of interferon-ß and rituximab on NLRP3 and AIM2 expression and IL-1β levels in patients with multiple sclerosis.","authors":"Sina Molavizade, Fereshteh Ashtari, Fateme Dehghani, Zahra Karimi, Mohammad Mahjoubi, Nasrin Zare","doi":"10.1080/15321819.2025.2534453","DOIUrl":"10.1080/15321819.2025.2534453","url":null,"abstract":"Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system. Inflammasomes, particularly NLRP3 and AIM2, have been implicated in MS pathogenesis. Interferon-β (IFN-β) and Rituximab (RTX) are treatment agents for relapsing-remitting MS (RRMS), but their impact on inflammasome regulation remains unclear. This study evaluates the expression of NLRP3 and AIM2 inflammasomes in MS patients responding to IFN-β and RTX, alongside newly diagnosed cases and healthy controls. Blood samples from IFN-β-treated patients (n = 23), RTX-treated patients (n = 23), newly diagnosed people (n = 20), and healthy controls (n = 12) were analysed. mRNA levels of NLRP3 and AIM2 were measured by RT-PCR, and plasma IL-1β was assessed using ELISA. Results revealed AIM2 expression was significantly higher in RTX-treated patients compared to other groups, while NLRP3 showed no significant differences. IL-1β levels were elevated in all patient groups, but no correlations were found between disease/treatment duration and inflammasome or IL-1β levels. RTX significantly increases AIM2 expression, suggesting its potential as a biomarker or therapeutic target in MS. Further research is needed to clarify AIM2's role in disease processes.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"450-466"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of analytical methods for detection of anti-drug antibodies neutralizing IL-15 and TGF-β RII activity. 中和IL-15和TGF-β RII活性的抗药物抗体检测方法的建立。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-08-07 DOI: 10.1080/15321819.2025.2538025

Gilles M Leclerc, Jack O Egan, Lijing You, Niraj Shrestha, Peter R Rhode, Hing C Wong

{"title":"Development of analytical methods for detection of anti-drug antibodies neutralizing IL-15 and TGF-β RII activity.","authors":"Gilles M Leclerc, Jack O Egan, Lijing You, Niraj Shrestha, Peter R Rhode, Hing C Wong","doi":"10.1080/15321819.2025.2538025","DOIUrl":"10.1080/15321819.2025.2538025","url":null,"abstract":"To comply with regulatory guidelines and ensure that biopharmaceutical agents meet safety and efficacy, we developed an analytical method to detect anti-drug antibodies (ADAs) generated in response to HCW9218, a fusion molecule containing the human soluble TGF-β RII and IL-15/IL-15 Rα sushi domains. The method demonstrated a sensitivity of 34.6 ng/mL with a suitably high degree of specificity, selectivity, and precision. To assess the neutralizing capacity of ADAs, we developed cell-based assays specific to IL-15 and TGF-β RII. These assays effectively discriminated the neutralizing activity of sera spiked with neutralizing antibody (NAb). The determination of positive NAb was based on absorbances corresponding to a threshold value of 30% inhibition of IL-15 or TGF-β RII activity. Each assay was validated using human sera from ongoing clinical trials. ADAs were detected in most sera tested with titers less than 10,000. None of the sera inhibited IL-15 and TGF-β RII activity by more than 30%. Interestingly, circulating TGF-β present in sera mimicked the action of NAb by binding to soluble TGF-β RII resulting in higher baseline neutralization activity. These methods proved to be suitable for detection of ADAs and NAbs in HCW9218 clinical samples or analogs sharing similar receptor/cytokine subunits and/or downstream signaling pathways.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"490-518"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occult hepatitis B and the detection of other HBV genes in HBs gene negative individuals in southwest Nigeria. 尼日利亚西南部HBs基因阴性个体隐匿性乙型肝炎及其他HBV基因的检测

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-06-12 DOI: 10.1080/15321819.2025.2516487

Olufisayo A Adesina, Favour A Babarinde, Dapo J Oluwajuyite, Oluwawemimo T Akinlabi

{"title":"Occult hepatitis B and the detection of other HBV genes in HBs gene negative individuals in southwest Nigeria.","authors":"Olufisayo A Adesina, Favour A Babarinde, Dapo J Oluwajuyite, Oluwawemimo T Akinlabi","doi":"10.1080/15321819.2025.2516487","DOIUrl":"10.1080/15321819.2025.2516487","url":null,"abstract":"Considering the high tendency of hepatitis B virus (HBV) to mutate and escape diagnosis, and the high prevalence of its chronic infection in Nigeria, it is expedient to wholistically study the various genes to understand the changes and to prepare for such to enhance the diagnosis, management, and control of the disease. This study was designed to detect the four genes of HBV in different categories of participants. Venous blood samples were collected from sick and apparently healthy individuals and screened for HBsAg using the ELISA method. Out of the samples, 36 hBsAg positive and 9 randomly selected HBsAg negative were selected for HBV DNA extraction and PCR using established primers and protocols. The HBsAg prevalence was found to be 13.8% (51/369) with Oyo state having the highest (37.3%; N = 19/51) compared to other states. After amplification, HBs gene detection was 28.9%; (n = 13/45), HBc gene 33.3%; (n = 15/45), HB Pol gene 26.7%; (n = 12/45), and HBx gene 35.6%; (n = 16/45) and one OBI case (11.1%; n = 1/9). Considering the complications associated to HBV infections and the various changes being observed in the structure of the virus, more study is needed to enhance the diagnosis, manage- 230 ment, and control of hepatitis B.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"467-477"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implication of TRPM8, CD47, and CDK4 expressions in hepatocellular carcinoma progression. TRPM8、CD47和CDK4表达在肝癌进展中的意义

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-02-16 DOI: 10.1080/15321819.2025.2464718

Aiat Shaban Hemida, Mona Saeed Tantawy

{"title":"Implication of TRPM8, CD47, and CDK4 expressions in hepatocellular carcinoma progression.","authors":"Aiat Shaban Hemida, Mona Saeed Tantawy","doi":"10.1080/15321819.2025.2464718","DOIUrl":"10.1080/15321819.2025.2464718","url":null,"abstract":"Participation of TRPM8 in hepatocellular carcinoma (HCC) development was not precisely declared. CD47 mediates immune escape and macrophage phagocytosis of tumors. CDK4 mediates oncogenesis. Synergistic implications of TRPM8, CD47, and CDK4 in HCC were not declared. This research aims to demonstrate the expressions of TRPM8, CD47and CDK4 in HCC and to declare correlations and significance. Paraffin blocks from 101 hCC and 82 adjacent non-tumorous liver were immunostained using TRPM8, CD47 and CDK4 antibodies. HCC showed highly significant increased TRPM8, CD47, and CDK4 expressions than control liver tissue (p < 0.001) for all. TRPM8, CD47, and CDK4 were significantly associated with poor prognostic criteria as high tumor grade, advanced stage, microvascular invasion, and necrosis. There was a significant association between cirrhotic and non-cirrhotic adjacent liver regarding positive TRPM8 (p < 0.02) and high CDK4 expressions (p < 0.045). There were significant direct relationships between each immunohistochemical antibody and the other two. Prolonged overall survival was significantly associated with low CDK4 (p = 0.019). In conclusion, TRPM8, CD47, and CDK4 may regulate synergistic functions in HCC oncogenesis and accomplish unfavorable prognostic significance. TRPM8 and CDK4 might share in development of HCC from cirrhosis. TRPM8, CD47, and CDK4 could be therapeutic targets in HCC.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"245-261"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lymphangioinvasion detection using the monoclonal antibody D2-40 (Podoplanin)as a clinical predictor of axillary lymph node metastasis in breast cancer patients. 使用单克隆抗体D2-40 （Podoplanin）检测淋巴管侵袭作为乳腺癌患者腋窝淋巴结转移的临床预测指标。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-02-23 DOI: 10.1080/15321819.2025.2470434

Teresa Cistina Ferreira Gutman, Angela de Salles Rezende, Dyego Mondego Moraes, Consuelo Lozoya Lopez, Licínio Esmeraldo da Silva, Rafaela Elvira Rozza-de-Menezes, João Paulo Lima Daher, André Vallejo da Silva, Fabiana Resende Rodrigues, Vânia Gloria Silami Lopes

{"title":"Lymphangioinvasion detection using the monoclonal antibody D2-40 (Podoplanin)as a clinical predictor of axillary lymph node metastasis in breast cancer patients.","authors":"Teresa Cistina Ferreira Gutman, Angela de Salles Rezende, Dyego Mondego Moraes, Consuelo Lozoya Lopez, Licínio Esmeraldo da Silva, Rafaela Elvira Rozza-de-Menezes, João Paulo Lima Daher, André Vallejo da Silva, Fabiana Resende Rodrigues, Vânia Gloria Silami Lopes","doi":"10.1080/15321819.2025.2470434","DOIUrl":"10.1080/15321819.2025.2470434","url":null,"abstract":"Breast is a major global health issue and the most common cancer in women. Identifying vascular invasion is challenging due to the need to distinguish true invasion from artifacts. This study explored lymphatic embolism in invasive breast carcinoma using the monoclonal antibody D2-40 as a prognostic indicator. A total of 100 patients with invasive breast carcinoma from 2009 to 2011 were included in the study. Tissue microarray technique (TMA) was used on patient tissue, constructing three paraffin blocks from each participant's histological data. Immunohistochemistry with D2-40 and CD34 antibodies was performed to identify lymphatic and blood emboli, respectively, and results were compared with previous findings. A prior report using hematoxylin-eosin staining found fewer patients with lymphatic emboli (34) compared to our study (56) using D2-40. Lymphatic emboli correlated with axillary metastases, with an odds ratio (OR) of 3.50, a 95% confidence interval (CI) of 1.92-5.08, and a p-value of 0.001, whereas hematoxylin-eosin alone showed OR = 1.42, 95% CI = 0.40-3.47, and p-value = 0.23. TMA with D2-40 staining detected more lymphatic emboli than hematoxylin-eosin staining alone. Higher embolic expression rates are linked to increased tumor aggressiveness, worse prognosis and shorter overall survival.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"274-288"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic significance of B-Catenin and E-Cadherin expression in gastric carcinoma. 胃癌中B-Catenin和E-Cadherin表达的预后意义。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-05-14 DOI: 10.1080/15321819.2025.2505033

Sarra Ben Rejeb, Abir Labadi, Marwa Lakhal, Khadija Bellil, Adnen Chouchen

{"title":"Prognostic significance of B-Catenin and E-Cadherin expression in gastric carcinoma.","authors":"Sarra Ben Rejeb, Abir Labadi, Marwa Lakhal, Khadija Bellil, Adnen Chouchen","doi":"10.1080/15321819.2025.2505033","DOIUrl":"10.1080/15321819.2025.2505033","url":null,"abstract":"Introduction: Gastric cancer (GC) is a leading cause of cancer-related mortality worldwide. Dysregulation of molecular pathways, including β-Catenin-mediated Wnt signaling, epithelial-to-mesenchymal transition (EMT), and E-Cadherin-modulated cell adhesion, plays critical roles in gastric carcinogenesis. This study assesses the expression patterns of β-Catenin and E-Cadherin in GC and explores their prognostic significance.Methods: This retrospective, multi-center study analyzed GC cases diagnosed between 2009 and 2019 at the pathology departments of Security Forces and Rabta Hospitals. Tissue microarray (TMA) paraffin blocks from 48 GC cases were immunohistochemically stained using antibodies for β-Catenin (Leica, 17C2) and E-Cadherin (Leica, 36B5). β-Catenin expression was scored as membranous, cytoplasmic, or nuclear, with overexpression defined as ≥ 50% positive cells. E-Cadherin staining was categorized from absent (score 0) to marked membranous staining (score 3), with scores 0-2 considered aberrant. Statistical analysis was performed using SPSS version 23.Results: Of the 48 cases, β-Catenin overexpression was observed in 50% of cases, significantly associated with tumor differentiation (p = 0.033), age > 60 years (p = 0.042), and male sex (p = 0.028). Aberrant E-Cadherin expression was found in 65% of cases, linked to poorly cohesive and diffuse subtypes (p = 0.053), poor differentiation (p = 0.042), and recurrence (p = 0.043), with a trend toward reduced survival (p = 0.056).Conclusion: β-Catenin overexpression and aberrant E-Cadherin expression are frequent in GC, reflecting their roles in tumor progression via Wnt signaling and EMT. These findings highlight their potential as prognostic biomarkers and therapeutic targets, particularly for Wnt pathway-directed therapies in personalized GC management.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"317-330"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical expression of epidermal growth factor receptor: prognostic value in HER2 positive breast cancer. 表皮生长因子受体的免疫组织化学表达：HER2阳性乳腺癌的预后价值。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-03-09 DOI: 10.1080/15321819.2025.2475291

Yoldez Houcine, Hend Ben Salem, Sirine El Fekih, Amal Maaoui, Maha Driss

{"title":"Immunohistochemical expression of epidermal growth factor receptor: prognostic value in HER2 positive breast cancer.","authors":"Yoldez Houcine, Hend Ben Salem, Sirine El Fekih, Amal Maaoui, Maha Driss","doi":"10.1080/15321819.2025.2475291","DOIUrl":"10.1080/15321819.2025.2475291","url":null,"abstract":"Introduction: Epidermal Growth Factor Receptor (EGFR) expression is not well-studied in Human Epidermal Growth Factor Receptor 2 (HER2) positive breast cancer. We aim to study the prevalence of EGFR immunohistochemical expression in HER2-positive breast carcinomas and to correlate this expression with different anatomo-clinical parameters.Methods: It was a retrospective study involving cases of HER2-positive breast carcinoma collected at the Immuno-Histo-Cytology Department of Salah Azaïez Institute of Tunis between 2018 and 2020. An immunohistochemical study using the anti-human EGFR monoclonal antibody was performed. Cases with an overall score ≥1+ were considered positive.Results: Fifty patients were included. EGFR expression in HER2-positive breast carcinomas was more likely to occur in patients under the age of 50 (p = 0.063). It was significantly associated with the absence of lymphovascular invasion (p = 0.047). In multivariate analysis, young age, absence of lympho-vascular invasion, and high Ki67 proliferation index (>60%) were independently associated with positive EGFR expression (p = 0.047, p = 0.040, and p = 0.050, respectively).Conclusion: Through this first Tunisian study, our data revealed that the immunohistochemical expression of EGFR is associated with young age, absence of lymphovascular invasion, and a high mitotic index (Ki67), which may suggest a potential predictive value for chemotherapy response.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"262-273"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implementation of TRAF3IP2 and SIGIRR gene polymorphisms and their expression levels in autoimmune thyroid diseases. 自身免疫性甲状腺疾病中TRAF3IP2和SIGIRR基因多态性及其表达水平的实现

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-05-29 DOI: 10.1080/15321819.2025.2511339

Mona A Abbas, Raouth E Girgis, Hytham R Badr, Ahmed E Abdel Meguid, Eman A E Badr

{"title":"Implementation of TRAF3IP2 and SIGIRR gene polymorphisms and their expression levels in autoimmune thyroid diseases.","authors":"Mona A Abbas, Raouth E Girgis, Hytham R Badr, Ahmed E Abdel Meguid, Eman A E Badr","doi":"10.1080/15321819.2025.2511339","DOIUrl":"10.1080/15321819.2025.2511339","url":null,"abstract":"Genetics plays a crucial role in the development of autoimmune thyroid diseases (AITDs), including Graves' disease (GD) and Hashimoto's thyroiditis (HT). This study evaluated the relationship between TRAF3IP2 and SIGIRR gene expression, their polymorphisms (rs13210247 and rs7396562, respectively), and AITDs risk. Gene expression and polymorphism genotyping were assessed by real-time PCR in 150 participants (50 GD, 50 HT, and 50 controls). TRAF3IP2 expression was considerably higher in GD and HT in contrast to controls. Regression analysis of TRAF3IP2 rs13210247 demonstrated a significant association with GD and HT risk. The AG genotype proved a considerable relationship with GD risk. At the same time, the AG genotype and the G allele exhibited a notable relationship with HT incidence. SIGIRR expression was notably downregulated in GD and HT versus controls. For rs7396562, the regression analysis demonstrated that the CA, AA, CA+AA genotypes, and A allele significantly correlated with GD risk. They are also notably linked with HT risk. We concluded that altered TRAF3IP2 and SIGIRR gene expression and their genetic variants may contribute to AITDs susceptibility.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"353-368"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0