Journal of immunoassay & immunochemistry最新文献_第5页

The role of SHMT2 and NDUFA4L2 gene expression and UCA1 levels in Egyptian patients with bladder cancer. SHMT2和NDUFA4L2基因表达和UCA1水平在埃及膀胱癌患者中的作用

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-07-14 DOI: 10.1080/15321819.2025.2533800

Eman A E Badr, Yostena Mekhail, Khaled S A Hassan, Mohamed F A Assar

{"title":"The role of SHMT2 and NDUFA4L2 gene expression and UCA1 levels in Egyptian patients with bladder cancer.","authors":"Eman A E Badr, Yostena Mekhail, Khaled S A Hassan, Mohamed F A Assar","doi":"10.1080/15321819.2025.2533800","DOIUrl":"10.1080/15321819.2025.2533800","url":null,"abstract":"Bladder Cancer (BC) has a significant burden, with poor survival rates in advanced cases. This case-controlled study aimed to identify genes having diagnostic and prognostic value. Seventy patients with BC and 70 healthy individuals were included. Real-time PCR was used to quantify the expression of the SHMT2 and NDUFA4L2 genes, and UCA1 levels. Results showed that SHMT2, NDUFA4L2, and UCA1 were significantly upregulated in patients compared to the control group (p < 0.001). The area under the receiver operating characteristic curve (ROC) analysis showed that AUC was 0.995 (95% CI = 0.989-1, p < 0.001), 0.994 (95% CI = 0.987-1, p < 0.001) and 0.977 (95% CI = 0.958-0.996, p < 0.001) for UCA1, SHMT2, and NDUFA4L2, respectively with high diagnostic sensitivity and specificity. Additionally, the high expression of SHMT2 and the high level of UCA1 were correlated with histological grade (p < 0.001and 0.001, respectively) and with CT tumor size (p = 0.005 and 0.001, respectively). In univariate COX regression analysis, SHMT2 and UCA1 were poor prognostic (p = 0.003 and 0.001, respectively). Furthermore, in the multivariate analysis, UCA1 was independent poor prognostic factor (p =0.037). Thus, SHMT2, NDUFA4L2, and UCA1 could be promising diagnostic and prognostic biomarkers and a novel therapeutic target.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"435-449"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seroprevalence and associated risk factors of Newcastle disease in guinea fowls in the Upper East region of Ghana. 加纳上东部地区珍珠鸡新城疫血清流行率及相关危险因素

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-06-12 DOI: 10.1080/15321819.2025.2518206

Albert Agyapong Tweneboah, Sherry Ama Mawuko Johnson, Patrick Mensah Amponsah, Derrick Adu Asare, Benjamin Obukowho Emikpe

{"title":"Seroprevalence and associated risk factors of Newcastle disease in guinea fowls in the Upper East region of Ghana.","authors":"Albert Agyapong Tweneboah, Sherry Ama Mawuko Johnson, Patrick Mensah Amponsah, Derrick Adu Asare, Benjamin Obukowho Emikpe","doi":"10.1080/15321819.2025.2518206","DOIUrl":"10.1080/15321819.2025.2518206","url":null,"abstract":"Newcastle Disease (ND) is a major constraint to poultry production in sub-Saharan Africa. However, information on Newcastle disease in guinea fowls is scant in Ghana. This study seeks to detect circulating antibodies against ND and the risk factors for occurrence in guinea fowls in the Upper East region of Ghana. Sera was obtained from guinea fowls from households, live bird markets, and slaughter points in different locations in the Upper East region. The sera were evaluated for antibodies against Newcastle disease using the Haemagglutination Inhibition (HI) test. Questionnaire was administered to the farmers to assess risk factors such as vaccination status, management system, and contact with wild birds. Almost half, 213/431 (49.4%, 95% CI = 44.6-54.2) of the guinea fowls were seropositive. The seroprevalence was 46.2% in live bird markets, 36.63% in households, and 52.7% at slaughter points. A seroprevalence of 49.7% was observed in adults and 45.7% growers. There was a 53.4% prevalence in males and 43.9% in females. Antibodies to ND were found circulating in guinea fowls in the Upper East region. We recommend that guinea fowls be made an important component element in the ND surveillance for an effective monitoring and control in the region.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"403-418"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CXCR4 expression in immunohistochemistry of gastrointestinal neuroendocrine neoplasms: a meta-analysis. CXCR4在胃肠道神经内分泌肿瘤免疫组织化学中的表达：一项meta分析。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-03-24 DOI: 10.1080/15321819.2025.2482642

Changlong Ruan, Jiajia Zhang, Yingying Liao, Yujie Hang, Yuan Dong, Hanfeng Shi, Xiaoya Wang

{"title":"CXCR4 expression in immunohistochemistry of gastrointestinal neuroendocrine neoplasms: a meta-analysis.","authors":"Changlong Ruan, Jiajia Zhang, Yingying Liao, Yujie Hang, Yuan Dong, Hanfeng Shi, Xiaoya Wang","doi":"10.1080/15321819.2025.2482642","DOIUrl":"10.1080/15321819.2025.2482642","url":null,"abstract":"Objective: C-X-C chemokine receptor type 4 (CXCR4) is significantly associated with the development of various malignant tumors. This meta-analysis aims to investigate the expression of CXCR4 in the immunohistochemistry of gastrointestinal neuroendocrine neoplasms (GI-NENs).Methods: A comprehensive literature search regarding gastrointestinal neuroendocrine neoplasms and CXCR4 was conducted using PubMed,Web of Science, and the Cochrane Library, with a cut off date of June 30, 2024.Two researchers independently screened the literature according to inclusion and exclusion criteria and assessed study quality using the Newcastle-OttawaScale. Meta-analysis was performed using Stata version 17.0. The pooled positive rate was employed to evaluate the expression of CXCR4 in the immunohistochemistry of GI-NENs.Results: A total of eight studies involving 501 patients were included in this research. The immunohistochemical analysis revealed positive CXCR4 expression in 174 patients. The meta-analysis calculated and summarized the combined positive rate (R: 0.41; 95% CI = 0.21-0.60, p = 0.00), indicating that the differences were statistically significant.Conclusion: CXCR4 is highly expressed in the immunohistochemistry of GI-NENs, which may provide some evidence for the therapeutic application of CXCR4 antagonists in treating neuroendocrine neoplasms.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"209-217"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of honey-ginger mouthwash on oral mucositis in patients undergoing chemotherapy. 蜂蜜姜漱口水对化疗患者口腔黏膜炎的影响。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-03-25 DOI: 10.1080/15321819.2025.2484645

Fatemeh Sadat Razavi, Hania Zokaee, Mojtaba Sehat, Mohsen Taghizadeh, Hossein Motedayyen, Elaheh Ghasemzadeh Hoseini

{"title":"Effect of honey-ginger mouthwash on oral mucositis in patients undergoing chemotherapy.","authors":"Fatemeh Sadat Razavi, Hania Zokaee, Mojtaba Sehat, Mohsen Taghizadeh, Hossein Motedayyen, Elaheh Ghasemzadeh Hoseini","doi":"10.1080/15321819.2025.2484645","DOIUrl":"10.1080/15321819.2025.2484645","url":null,"abstract":"Background: Oral mucositis is considered as one of the most prevalent complications of chemotherapy or radiation therapy in cancerous tumors, which can interrupt the patient's treatment and nutrition. This study therefore aimed to evaluate the efficacy of ginger-honey mouthwash on the prevention of chemotherapy-induced oral mucositis in patients suffering from various cancers.Materials and methods: In this randomized clinical trial study, 70 patients receiving chemotherapy were divided into case and control groups. The former group (n = 34) received natural honey-ginger mouthwash and the latter (n = 36) used normal saline for 14 days. The presence and severity of oral mucositis, pain intensity, and other related characteristics were evaluated based on a two-part questionnaire (demographic and clinical information) and a checklist prepared from the protocols of the World Health Organization in each group.Results: During a 14-day intervention, patients received a 7-day intervention with ginger-honey mouthwash revealed a significant reduction in the mean severity of oral mucositis compared to the control group (p = 0.03). However, a 14-day intervention with ginger-honey mouthwash indicated no significant impact on the mean severity of oral mucositis (p = 0.6). In addition, no significant difference was observed in pain intensity between case and control groups during these 14 days.Conclusions: This study suggests that a seven-day intervention with ginger-honey mouthwash has a beneficial effect on reducing the severity of mucositis in patients under chemotherapy, unlike a 14-day intervention. The honey-ginger mouthwash fails to have a significant effect on the pain intensity due to mucositis in patients undergoing chemotherapy.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"303-315"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative immunodiagnostic performance of AgB1-derived synthetic peptides in human cystic echinococcosis. agb1合成肽对人囊性包虫病的比较免疫诊断性能。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-09-05 DOI: 10.1080/15321819.2025.2556426

Javier Magnone, Maite Folle, Sebastián Miles, Sofía Lagos, María Clara González-Porcile, Ana Hernández, Daniel Da-Rosa, Ana María Ferreira, Cecilia Sóñora, Gustavo Mourglia-Ettlin

{"title":"Comparative immunodiagnostic performance of AgB1-derived synthetic peptides in human cystic echinococcosis.","authors":"Javier Magnone, Maite Folle, Sebastián Miles, Sofía Lagos, María Clara González-Porcile, Ana Hernández, Daniel Da-Rosa, Ana María Ferreira, Cecilia Sóñora, Gustavo Mourglia-Ettlin","doi":"10.1080/15321819.2025.2556426","DOIUrl":"10.1080/15321819.2025.2556426","url":null,"abstract":"Immunoassays are complementary diagnostic tools in human cystic echinococcosis (CE) despite sensitivity/specificity limitations, and synthetic peptides have been suggested to potentially overcome disadvantages reported for traditional antigens. Herein, a systematic study comparing the immunodiagnostic performance of AgB1 versus synthetic peptides derived from its sequence was carried out. Thus, a eukaryotic-expressed recombinant AgB1 was assessed, together with a reported synthetic peptide (p176, N-terminal portion of AgB1) and two new peptides within p176 (namely pB1a and pB1b) corresponding to predicted linear B-cell epitopes. Immunodiagnostic performances were evaluated by ELISA using a large collection of human sera from CE patients, healthy donors, and individuals with other parasitoses; assessing the sensitivity, specificity, indeterminacy, cross-reactivity, and diagnostic efficiency of the assay according to each antigen. Results suggest that peptides retaining most of the original protein sequence and 3D-structure display better overall diagnostic efficiencies (IgGt values for rAgB1, p176, pB1a, and pB1b were 66.1%, 60.4%, 54.7%, and 49.0%, respectively), with a small N-terminal portion of AgB1 being immunodominant. Additionally, detection of specific IgG4 antibodies significantly reduced cross-reactivity, while improving sensitivity (IgGt-IgG4 values for rAgB1, p176, and pB1a were 37.5%-42.7%, 24.0%-29.2%, and 11.5%-24.0%, respectively). Valuable information was obtained for rationally design novel peptide-based assays for CE immunodiagnosis.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"605-618"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of IL2-330 (rs2069762) gene polymorphism in Egyptian patients with lung cancer: a case-control study. IL2-330 （rs2069762）基因多态性在埃及肺癌患者中的研究：一项病例对照研究

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-03-18 DOI: 10.1080/15321819.2025.2477605

Esraa Tawfik Allam, Mohamed S El Senbawy, Hanaa M Elgazzar, Inas Moaz, Sara Ahmed Saied, Hind S AboShabaan, Shimaa Mohammed Hamad, Samah Ahmed Elbeltagy, Omnia S Nabih

{"title":"Study of IL2-330 (rs2069762) gene polymorphism in Egyptian patients with lung cancer: a case-control study.","authors":"Esraa Tawfik Allam, Mohamed S El Senbawy, Hanaa M Elgazzar, Inas Moaz, Sara Ahmed Saied, Hind S AboShabaan, Shimaa Mohammed Hamad, Samah Ahmed Elbeltagy, Omnia S Nabih","doi":"10.1080/15321819.2025.2477605","DOIUrl":"10.1080/15321819.2025.2477605","url":null,"abstract":"Background: IL2 is one of the key cytokines essential for regulating the immune system and the inflammation-related carcinogenesis process. Few studies have examined the relationship between lung cancer and the IL2-330 (rs2069762) gene polymorphism, despite several studies demonstrating that it is linked to numerous cancer types.Objective: Our study aimed to investigate the association between IL2-330 (rs2069762) polymorphism and lung cancer risk and explore the role of IL2-330 polymorphism in survival outcomes (OS and PFS).Patients and methods: The study was conducted from October 2023 to November 2024, including 50 randomly selected patients diagnosed with lung cancer and 50 subjects matched for age and gender were used as controls in this case-control study. The IL2-330 (rs2069762) gene polymorphism was assessed using real-time PCR.Results: We found that the AC genotype was associated with a notably lower risk of lung cancer in comparison to the AA genotype (95% CI: 0.06-0.61, p = 0.01). Conversely, the CC genotype showed no significant association with lung cancer risk when compared to the reference genotype. The comprehensive comparison of survival distributions among the AA, AC, and CC genotypes through the Log-Rank (Mantel-Cox) test indicated no statistically significant difference.Conclusions: According to our research, The AC genotype of IL2-330 (rs2069762) is associated with a significantly higher survival rate and lower risk of lung cancer. Further future studies are needed to confirm these findings.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"289-302"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ki67 immunoexpression in different histological grades of Oral squamous cell carcinoma: a retrospective study. Ki67免疫表达在不同组织学级别口腔鳞状细胞癌中的回顾性研究。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-10-25 DOI: 10.1080/15321819.2025.2578769

Rashmi Wankhade, Pratibha Dawande, Nandkishor Bankar

{"title":"Ki67 immunoexpression in different histological grades of Oral squamous cell carcinoma: a retrospective study.","authors":"Rashmi Wankhade, Pratibha Dawande, Nandkishor Bankar","doi":"10.1080/15321819.2025.2578769","DOIUrl":"10.1080/15321819.2025.2578769","url":null,"abstract":"Oral squamous cell carcinoma (OSCC) accounts for over 90% of all oral malignancies and has a 50-60% five-year survival rate, despite advances in treatment. Ki67, a nuclear protein involved in cellular proliferation, has been studied as a prognostic marker in various malignancies, including OSCC. However, its link with different histological grades of OSCC is uncertain. The study aimed to evaluate Ki-67 expression in well-differentiated (WDOSCC), moderately differentiated (MDOSCC), and poorly differentiated (PDOSCC) tumors to ascertain its correlation with tumor differentiation and aggressiveness. Forty OSCC cases were classified using Broder's histological grading system. Ki67 immunohistochemical staining was performed, and three groups were categorized based on Ki67 expression: low (1-25%), moderate (26-50%), and high ( > 50%) proliferation. Statistical analysis assessed the significance of Ki67 expression across OSCC grades. Among 40 cases, 35% were WDOSCC, 45% MDOSCC, and 20% PDOSCC. Low Ki67 proliferation was seen in 64% of WDOSCC, moderate in 61% of MDOSCC, and high in 75% of PDOSCC. There was a significant (p < 0.001) association between OSCC grading and Ki67 expression. Ki67 expression correlates with OSCC histological grades, increasing with tumor proliferation. These findings support Ki67 as a prognostic marker, warranting further large-scale validation studies.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"588-604"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145368097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SARS-CoV-2 nucleocapsid detection using a recombinant phage display-isolated single-chain fragment variable. 利用重组噬菌体展示分离单链片段变量检测SARS-CoV-2核衣壳。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-03-24 DOI: 10.1080/15321819.2025.2483839

Abbasali Salarifar, Mohammad Javad Rasaee

{"title":"SARS-CoV-2 nucleocapsid detection using a recombinant phage display-isolated single-chain fragment variable.","authors":"Abbasali Salarifar, Mohammad Javad Rasaee","doi":"10.1080/15321819.2025.2483839","DOIUrl":"10.1080/15321819.2025.2483839","url":null,"abstract":"Background: Diagnosis is an important factor in controlling disease. Single-chain fragment variables (scFvs) can be used for diagnosis; however, due to their immobilization issues, their application has been limited. Herein, we isolated a SARS-CoV-2 nucleocapsid phosphoprotein (NP)-specific scFv and propose it as a diagnostic tool in the scFv-displaying phage format to overcome the immobilization issue.Method: Spleen from NP-immunized BALB/c mice was isolated, total RNA was extracted, and cDNA was synthesized. An scFv library was constructed, using the splicing by overlap extension (SOE) PCR technique, which was cloned into the pCANTAB5E phagemid. The phage library was panned against the NP antigen, and the output phages with the highest binding capability were screened for the most qualified scFv, which was later assessed in terms of sensitivity and specificity.Results: The scFv-displaying phage library was panned against the recombinant NP in three rounds and 40 randomly selected colonies from the third round's outputs were screened. Alongside several clones, clone #31 was chosen as the most qualified scFv, which later exhibited favorable sensitivity and specificity against NP in further ELISA-based experiments.Conclusions: Clone #31 could be utilized to develop diagnostic tools and therapeutics against SARS-CoV-2.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"369-387"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between autoimmunity and COPD: an investigation based on proteomic profiling and antinuclear antibody screening. 自身免疫与慢性阻塞性肺病的关系：基于蛋白质组学分析和抗核抗体筛选的研究。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-05-06 DOI: 10.1080/15321819.2025.2502430

Sonu Das, Supriya Adiody, Jinsu Varghese, Mathew John

{"title":"Relationship between autoimmunity and COPD: an investigation based on proteomic profiling and antinuclear antibody screening.","authors":"Sonu Das, Supriya Adiody, Jinsu Varghese, Mathew John","doi":"10.1080/15321819.2025.2502430","DOIUrl":"10.1080/15321819.2025.2502430","url":null,"abstract":"Chronic Obstructive Pulmonary Disease is a major global health concern with significant morbidity and mortality, characterized by heterogeneity influenced by inflammation, oxidative stress, and autoimmunity. This study investigated the role of autoimmunity in stable and exacerbated COPD phenotypes using proteomic and immunological analyses to elucidate molecular mechanisms and identify potential biomarkers. Plasma samples from COPD phenotypes and healthy controls were analyzed using label-free mass spectrometry to identify differentially expressed proteins. Functional annotation and pathway enrichment analysis highlighted proteins linked to autoimmunity, while immunological assays assessed anti-nuclear antibody prevalence and intensity using ANA ELISA and indirect immunofluorescence. The study identified differentially expressed proteins, namely, DNA repair protein XRCC2, phosphatidyl inositol glycan-specific phospholipase D, E3 ubiquitin protein ligase SHPRH, and Protocadherin-β, implicated in autoimmune pathways. Pathway enrichment analysis of these proteins highlighted the uPAR-mediated signaling, mTOR, PI3K/Akt, ARF6, and S1P signaling pathways, known for their roles in autoimmunity. Immunological assays revealed ANA positivity in 47% of stable COPD and 36% of exacerbated COPD, among which, 80% exhibited a speckled fluorescence pattern, often associated with anti-SSA and anti-SSB antibodies. The findings highlight the potential role of autoimmunity in COPD pathogenesis, suggesting phenotype-specific immune dysregulation, providing a basis for future biomarker and therapeutic research.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"339-352"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative-antioxidative balance and stromal cell-derived factor-4 in generalized anxiety disorder: implications for pathophysiology and biomarker potential. 广泛性焦虑障碍的氧化-抗氧化平衡和基质细胞衍生因子-4：病理生理学和生物标志物潜能的含义。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-10-06 DOI: 10.1080/15321819.2025.2567856

Sidika Baziki Çetİn, Ülker Atilan Fedai, Öznur Akil, İsmail Koyuncu, Merve Doğan Koçbaba

{"title":"Oxidative-antioxidative balance and stromal cell-derived factor-4 in generalized anxiety disorder: implications for pathophysiology and biomarker potential.","authors":"Sidika Baziki Çetİn, Ülker Atilan Fedai, Öznur Akil, İsmail Koyuncu, Merve Doğan Koçbaba","doi":"10.1080/15321819.2025.2567856","DOIUrl":"10.1080/15321819.2025.2567856","url":null,"abstract":"This study explores the relationship between oxidative - antioxidative balance and stromal cell-derived factor-4 (SDF-4) levels in individuals with Generalized Anxiety Disorder (GAD), aiming to clarify their roles in the disorder's pathophysiology. By examining these biomarkers, the research investigates whether oxidative stress mechanisms contribute to GAD. A cross-sectional study was conducted with 43 GAD patients and 40 age- and sex-matched healthy controls. Participants were assessed using the Beck Anxiety Inventory (BAI) and the Structured Clinical Interview for DSM-5. Serum levels of SDF-4, total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were measured. GAD patients showed significantly higher levels of SDF-4, TAS, TOS, and OSI compared to controls (p < 0.001). No significant differences were found in other hematological parameters. In the GAD group, SDF-4 levels were not significantly correlated with anxiety severity or oxidative markers. ROC analysis indicated good diagnostic performance of SDF-4 for GAD (AUC = 0.870). The findings suggest that oxidative - antioxidative imbalance and elevated SDF-4 levels may play a role in GAD. SDF-4 might represent a compensatory response to oxidative stress. Elevated TAS levels may indicate enhanced antioxidant defense. Further studies are needed to clarify the mechanistic and therapeutic implications.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"654-666"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0