Journal of immunoassay & immunochemistry最新文献_第6页

Comparison of analysis of serum free light chains in a cartridge-based protein analyzer Mispa i3 with Roche Cobas 8000. Mispa i3蛋白分析仪与罗氏Cobas 8000蛋白分析仪血清游离轻链分析的比较

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-05-08 DOI: 10.1080/15321819.2025.2502433

Abhijith B L, Thushara Thomas, Dinimol Daniel, Jofy K Paul, D M Vasudevan

{"title":"Comparison of analysis of serum free light chains in a cartridge-based protein analyzer Mispa i3 with Roche Cobas 8000.","authors":"Abhijith B L, Thushara Thomas, Dinimol Daniel, Jofy K Paul, D M Vasudevan","doi":"10.1080/15321819.2025.2502433","DOIUrl":"10.1080/15321819.2025.2502433","url":null,"abstract":"Serum-free light-chain assays are important in the diagnosis and in monitoring therapeutic responses of plasma cell disorders and are complementary to serum protein electrophoresis. The serum-free light-chain assay detects the light-chain portion of immunoglobulin in its free form with high sensitivity. In combination with serum protein electrophoresis and serum immunofixation electrophoresis, the free light-chain assay serves an important role in predicting disease progression in monoclonal gammopathy. Here, we compare the performance of a cartridge-based system Mispa i3 using Diazyme reagent in comparison to Roche Cobas using Freelite reagent. Both of these reagents use polyclonal antibodies for the detection of serum-free light chains. Mispa i3 is a cartridge-based protein analyzer and has a unique channel shifting technology with both turbidimetric and nephelometric principles for immuno assays. Samples of 196 patients were included in this study, and very good agreement was observed between these two assays. Our data show that even though discrepancies were observed for high concentration samples, they are clinically correlated by the free light-chain ratios. We observed a very good concordance of 89% between these two assays for free light-chain ratios.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"478-489"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An indirect competitive ELISA assay using fragment antigen-binding (Fab) antibody for the quantitative detection of delta-9-tetrahydrocannabinol from Cannabis sativa L. 采用片段抗原结合抗体（Fab）间接竞争ELISA法定量检测大麻中δ -9-四氢大麻酚。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-07-27 DOI: 10.1080/15321819.2025.2538777

Worapol Sae-Foo, Jakkapat Paluka, Pewpan Maleewong Intapan, Waraporn Putalun

{"title":"An indirect competitive ELISA assay using fragment antigen-binding (Fab) antibody for the quantitative detection of delta-9-tetrahydrocannabinol from Cannabis sativa L.","authors":"Worapol Sae-Foo, Jakkapat Paluka, Pewpan Maleewong Intapan, Waraporn Putalun","doi":"10.1080/15321819.2025.2538777","DOIUrl":"10.1080/15321819.2025.2538777","url":null,"abstract":"Delta-9-tetrahydrocannabinol (THC) is one of the cannabinoid metabolites present in Cannabis sativa. Currently, cannabis is an ingredient in foods, cosmetics, and medicinal products. Many countries control cannabis and THC for illegal or under-regulation products. This study focused on development of recombinant antibodies specific to THC, and their application of analytical methods using enzyme-linked immunosorbent assay (ELISA) techniques. Conjugation of THC with bovine serum albumin or ovalbumin was performed using Mannich and N,N'-carbonyldiimidazole reactions. The recombinant antibody was expressed from Escherichia coli in form of fragmented antigen-binding (Fab) antibody. Antigen-conjugates and Fab were performed using ELISA-based analytical method and validated method for sensitivity, accuracy, and precision. The developed Fab in this study demonstrates high specificity for detection of THC. The analytical range was between 0.78 and 25 µg/mL, with limit of detection of 0.59 µg/mL. Accuracy ranged from 98.15-108.97%, with intra- and inter-assay % relative standard deviation of 2.15-8.03 and 1.83-8.45%, respectively. Parameters for method validation, including accuracy and precision, were within acceptable limits which are comparable to modified HPLC method from previous study. The approach demonstrated outstanding sensitivity and specificity for analysis of THC in cannabis extracts, suggesting it to be suitable for the quantitative assessment of THC in variety of products.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"535-556"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BRAF V600E protein detection by immunohistochemistry in metastatic colorectal cancer tissue: association with tumor characteristics and KRAS mutation. 转移性结直肠癌组织中BRAF V600E蛋白的免疫组化检测：与肿瘤特征和KRAS突变的关系

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-03-26 DOI: 10.1080/15321819.2025.2484408

Sarra Ben Rejeb, Safia Sakly, Rym Bahloul, Adnen Chouchen

{"title":"BRAF V600E protein detection by immunohistochemistry in metastatic colorectal cancer tissue: association with tumor characteristics and KRAS mutation.","authors":"Sarra Ben Rejeb, Safia Sakly, Rym Bahloul, Adnen Chouchen","doi":"10.1080/15321819.2025.2484408","DOIUrl":"10.1080/15321819.2025.2484408","url":null,"abstract":"Immunohistochemistry (IHC) using the BRAF V600E antibody is a sensitive and specific method for detecting BRAF V600E mutations in colorectal cancer (CRC). Given that BRAF and KRAS mutations are mutually exclusive, this study aimed to assess the expression of BRAF V600E in CRC according to KRAS mutation status. Automated IHC analysis was performed on tissue samples from metastatic CRC patients diagnosed between 2012 and 2022 using the anti-BRAF V600E antibody (GenomeME, IHC600). Positive and negative control tissues were used for validation. Cytoplasmic staining was considered positive, with intensity classified as weak, moderate, or strong. The percentage of positive tumor cells was recorded semi-quantitatively. Thirty-five cases were included.The mean age of patients was 60 years (±12.41), with a male-to-female ratio of 2.9. KRAS mutations were present in 48% of cases. BRAF V600E cytoplasmic staining was observed in 37.1%, with a mean percentage of positive cells of 50% (range: 5%-100%). Diffuse and focal staining were found in 7/13 and 6/13 cases, respectively. Significant associations were observed with mucinous carcinoma subtype, invasion patterns, and lymph node metastasis. All KRAS-mutated cases showed negative BRAF staining. Complete absence of BRAF V600E IHC staining in CRC is strongly associated with KRAS mutation, suggesting IHC as an efficient tool to predict KRAS mutational status.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"232-244"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seroprevalence of H9N2 and H5 avian influenza in mixed-species poultry farms in Northern Benin. 贝宁北部混种家禽养殖场H9N2和H5禽流感的血清流行率

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-04-24 DOI: 10.1080/15321819.2025.2496480

Edmond Onidje, Oluwole Oyetunde Oni, Mireille Catherine Kadja, Modupe Beatrice Abraham, Vitus Burimuah, Amponsah Patrick Mensah, Derrick Adu Asare, Johnson Opoku Bannor, Benjamin Obukowho Emikpe

{"title":"Seroprevalence of H9N2 and H5 avian influenza in mixed-species poultry farms in Northern Benin.","authors":"Edmond Onidje, Oluwole Oyetunde Oni, Mireille Catherine Kadja, Modupe Beatrice Abraham, Vitus Burimuah, Amponsah Patrick Mensah, Derrick Adu Asare, Johnson Opoku Bannor, Benjamin Obukowho Emikpe","doi":"10.1080/15321819.2025.2496480","DOIUrl":"10.1080/15321819.2025.2496480","url":null,"abstract":"Avian influenza is one of the major threats to poultry and human health in northern Benin, while mixed-species farming systems increase the risks of viral transmission. The present study estimated the seroprevalence of avian influenza subtypes H9N2 and H5 in indigenous chickens and guinea fowls in the Atacora and Donga regions. A total of 300 birds including 191 indigenous chickens and 109 guinea fowls, from six districts were sampled through a cross-sectional survey using systematic random sampling. Hemagglutination inhibition assay was used to detect antibodies, revealing an overall H9N2 seroprevalence of 41%, with 17.5% of samples testing positive for H5. The seroprevalence of H9N2 was notably higher in guinea fowls (51.81% in Atacora and 52% in Donga) compared to chickens (34.95% in Atacora and 34.83% in Donga). H5 antibodies were found only in guinea fowls in Atacora (46.66%). The study also found that farms with both chickens and guinea fowls had a significantly higher odds ratio for H9N2 positivity (OR = 4.25, p < 0.001) compared to chicken-only farms. The results underscore the importance of mixed-species systems in the transmission of avian influenza, suggesting that targeted surveillance and biosecurity measures are essential for controlling the spread of these viruses.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"419-433"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical GATA3 expression in prostate adenocarcinoma: pitfalls. 免疫组化GATA3在前列腺癌中的表达：陷阱。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-04-27 DOI: 10.1080/15321819.2025.2499640

Sarra Ben Rejeb, Yasmine Chaabane, Kalthoum Dridi, Rym Bahloul, Hassen Khouni

{"title":"Immunohistochemical GATA3 expression in prostate adenocarcinoma: pitfalls.","authors":"Sarra Ben Rejeb, Yasmine Chaabane, Kalthoum Dridi, Rym Bahloul, Hassen Khouni","doi":"10.1080/15321819.2025.2499640","DOIUrl":"10.1080/15321819.2025.2499640","url":null,"abstract":"Background: Recent studies have reported aberrant GATA3 immunohistochemical expression in high-grade prostate carcinoma, raising concerns about its specificity. This study aimed to evaluate GATA3 immunohistochemical expression in prostate adenocarcinoma.Methods: We retrospectively collected cases of primary prostate adenocarcinoma diagnosed in our pathology department(2019-2024). Clinical and pathological data, including Gleason and ISUP grades, were collected.An automated immunohistochemical analysis was performed using the anti-GATA3 antibody(Leica, L50-823). Only nuclear staining was considered positive.Results: Fifty-eight cases have been included in the study with a mean age of 67.8 years-old. GATA3 nuclear expression was observed in 6.9% (4/58) of cases, all classified as Gleason 7 (4 + 3) and ISUP grade 3. Only the Gleason 4 or 5 components showed staining, while Gleason 3 components were negative. Additionally, benign prostate glands and seminal vesicles showed rare GATA3 expression (4/58 and 1/58 cases, respectively).Conclusion: While GATA3 is commonly used as a marker for urothelial carcinoma, our findings indicate that it can also be expressed in prostate adenocarcinoma, particularly in higher Gleason grade components, which may pose a diagnostic pitfall. Further studies are needed to determine the clinical significance of GATA3 expression in prostate adenocarcinoma and its potential impact on tumor behavior and prognosis.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"331-338"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance of a point of care allergy test compared to an established laboratory test for specific IgE in Japanese patients with allergic rhinitis. 日本变应性鼻炎患者特异性IgE的护理点过敏测试与既定实验室测试的比较

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-04-16 DOI: 10.1080/15321819.2025.2490541

Tomohiro Ono, Tadamitsu Morimoto, Tomoya Hanakita, Kizuki Watanabe, Chie Iwamura, Natsuko Asakura

{"title":"Performance of a point of care allergy test compared to an established laboratory test for specific IgE in Japanese patients with allergic rhinitis.","authors":"Tomohiro Ono, Tadamitsu Morimoto, Tomoya Hanakita, Kizuki Watanabe, Chie Iwamura, Natsuko Asakura","doi":"10.1080/15321819.2025.2490541","DOIUrl":"10.1080/15321819.2025.2490541","url":null,"abstract":"Background: Pollinosis is highly prevalent. Reliable specific IgE tests are needed to identify the causative aeroallergens. This study aims to evaluate the performance of DropScreenTM (DS), a point of care test, by comparing it with that of ViewAllergy39TM, a laboratory test (VA).Methods: Sera from 70 Japanese outpatients with pollinosis (median age: 29.5 years; 35% were men) were used. Qualitative analysis and concordance rate assessment were performed. For allergens with discrepant results between DS and VA, inhibition tests were carried out on relevant samples and allergen component analysis for specific allergens was conducted.Results: The average positivity rates for the 17 allergens included in the study were 20.8% and 26.6% for DS and VA, respectively. DS positive rate for Japanese cypress was 10-fold lower than that of VA. Clear discrepancies in detection rates between VA and DS were also shown for Japanese cedar, Moth, and A. fumigatus. Inhibition tests on discrepant sera confirmed that VA could accurately measure specific IgE toward these allergens.Conclusion: DS and VA were not equally effective in reliably detecting specific IgE toward Japanese cypress. This study highlights the importance of understanding the impact of testing performance differences between assays on diagnostic workup and subsequent therapy selection.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"388-402"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Costus speciosus extract: a natural immunomodulator for treatment of psoriasis in BALB/c mice by NF-κB pathway. 木香提取物：经NF-κB通路治疗BALB/c小鼠银屑病的天然免疫调节剂

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-05-02 DOI: 10.1080/15321819.2025.2498439

Aditi Vyas, Muqtada Shaikh, Radhika Raheja, Gaurav Doshi

{"title":"Costus speciosus extract: a natural immunomodulator for treatment of psoriasis in BALB/c mice by NF-κB pathway.","authors":"Aditi Vyas, Muqtada Shaikh, Radhika Raheja, Gaurav Doshi","doi":"10.1080/15321819.2025.2498439","DOIUrl":"10.1080/15321819.2025.2498439","url":null,"abstract":"Psoriasis is a chronic inflammatory skin condition. Ayurveda, Traditional Chinese therapy, and Unani offer a potential approach to treating psoriasis. This study explores the immunomodulatory effects of Costus speciosus petroleum ether extract (CSPE) on psoriasis-like symptoms in Balb/c mice. GC-MS analysis was done to detect bioactive phytoconstituents in the extract. Mice were treated with Imiquimod (IMQ) to induce psoriasis-like symptoms. Measurements of back skin thickness, skin length, mass, and body weight were used in the study, with an assessment of the Psoriasis Area and Severity Index (PASI). The treatment group received doses of 110 mg/kg, 220 mg/kg, and 440 mg/kg of extracts. Nuclear Factor kappa-B (NF-kB), tumor necrosis factor-alpha (TNF-α), and interleukin-17 (IL-17) were estimated. Presence of diosgenin, tigogenin, aglycone of diosgenin, santamarine and reynosin in the extract was confirmed by GC-MS analysis. At 440 mg/kg, a significant effect was observed in the IMQ model. The extract significantly reduced levels of NF-kB, TNF-α, and IL-17. The research findings indicate that CSPE is a promising candidate for psoriasis treatment.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"557-577"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-chain variable fragment antibodies targeting the antileukemia agent harringtonine for enzyme-linked immunosorbent assay development. 单链可变片段抗体靶向抗白血病药物哈林丁碱酶联免疫吸附试验的发展。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-07-09 DOI: 10.1080/15321819.2025.2526208

Seiichi Sakamoto, Shohei Komatsu, Ai Moriyasu, Gorawit Yusakul, Waraporn Putalun, Poomraphie Nuntawong, Hiroyuki Tanaka, Satoshi Morimoto

{"title":"Single-chain variable fragment antibodies targeting the antileukemia agent harringtonine for enzyme-linked immunosorbent assay development.","authors":"Seiichi Sakamoto, Shohei Komatsu, Ai Moriyasu, Gorawit Yusakul, Waraporn Putalun, Poomraphie Nuntawong, Hiroyuki Tanaka, Satoshi Morimoto","doi":"10.1080/15321819.2025.2526208","DOIUrl":"10.1080/15321819.2025.2526208","url":null,"abstract":"In this study, we generated a single-chain variable fragment (scFv) specific to a potent antileukemic substance, harringtonine (HT) (HT-scFv) that can be used in enzyme-linked immunosorbent assay (ELISA) for the quantitative analysis of HT in the plant genus Cephalotaxus. The variable heavy (VH) and light chain (VL) genes were directly cloned from the cDNA of the hybridoma cell line 1D2, which secretes monoclonal antibody against HT (MAb 1D2). These genes were then assembled with a flexible peptide linker, specifically (Gly4Ser)3, through splicing by overlap extension PCR. The resulting HT-scFv gene was expressed in Escherichia coli. The denatured HT-scFv, produced as inclusion bodies, was solubilized and refolded using a dilution method to restore its functionality as an antibody. Characterization of the HT-scFv demonstrated its high specificity for HT. Additionally, its remarkable properties facilitated the development of an ELISA for HT detection, achieving a limit of detection (LOD) of 12.2 ng/mL. Furthermore, validation analyses showed that the ELISA using HT-scFv exhibited good accuracy and reliability for the quantitative assessment of HT in C. harringtonia \"Fastigiata.\" This study highlights the potential of HT-scFv as a valuable tool for ELISA in the quantitative analysis of HT.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"519-534"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the impact of interferon-ß and rituximab on NLRP3 and AIM2 expression and IL-1β levels in patients with multiple sclerosis. 评估干扰素β和利妥昔单抗对多发性硬化症患者NLRP3和AIM2表达及IL-1β水平的影响。

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-07-17 DOI: 10.1080/15321819.2025.2534453

Sina Molavizade, Fereshteh Ashtari, Fateme Dehghani, Zahra Karimi, Mohammad Mahjoubi, Nasrin Zare

{"title":"Assessment of the impact of interferon-ß and rituximab on NLRP3 and AIM2 expression and IL-1β levels in patients with multiple sclerosis.","authors":"Sina Molavizade, Fereshteh Ashtari, Fateme Dehghani, Zahra Karimi, Mohammad Mahjoubi, Nasrin Zare","doi":"10.1080/15321819.2025.2534453","DOIUrl":"10.1080/15321819.2025.2534453","url":null,"abstract":"Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system. Inflammasomes, particularly NLRP3 and AIM2, have been implicated in MS pathogenesis. Interferon-β (IFN-β) and Rituximab (RTX) are treatment agents for relapsing-remitting MS (RRMS), but their impact on inflammasome regulation remains unclear. This study evaluates the expression of NLRP3 and AIM2 inflammasomes in MS patients responding to IFN-β and RTX, alongside newly diagnosed cases and healthy controls. Blood samples from IFN-β-treated patients (n = 23), RTX-treated patients (n = 23), newly diagnosed people (n = 20), and healthy controls (n = 12) were analysed. mRNA levels of NLRP3 and AIM2 were measured by RT-PCR, and plasma IL-1β was assessed using ELISA. Results revealed AIM2 expression was significantly higher in RTX-treated patients compared to other groups, while NLRP3 showed no significant differences. IL-1β levels were elevated in all patient groups, but no correlations were found between disease/treatment duration and inflammasome or IL-1β levels. RTX significantly increases AIM2 expression, suggesting its potential as a biomarker or therapeutic target in MS. Further research is needed to clarify AIM2's role in disease processes.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"450-466"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occult hepatitis B and the detection of other HBV genes in HBs gene negative individuals in southwest Nigeria. 尼日利亚西南部HBs基因阴性个体隐匿性乙型肝炎及其他HBV基因的检测

Journal of immunoassay & immunochemistry Pub Date : 2025-01-01 Epub Date: 2025-06-12 DOI: 10.1080/15321819.2025.2516487

Olufisayo A Adesina, Favour A Babarinde, Dapo J Oluwajuyite, Oluwawemimo T Akinlabi

{"title":"Occult hepatitis B and the detection of other HBV genes in HBs gene negative individuals in southwest Nigeria.","authors":"Olufisayo A Adesina, Favour A Babarinde, Dapo J Oluwajuyite, Oluwawemimo T Akinlabi","doi":"10.1080/15321819.2025.2516487","DOIUrl":"10.1080/15321819.2025.2516487","url":null,"abstract":"Considering the high tendency of hepatitis B virus (HBV) to mutate and escape diagnosis, and the high prevalence of its chronic infection in Nigeria, it is expedient to wholistically study the various genes to understand the changes and to prepare for such to enhance the diagnosis, management, and control of the disease. This study was designed to detect the four genes of HBV in different categories of participants. Venous blood samples were collected from sick and apparently healthy individuals and screened for HBsAg using the ELISA method. Out of the samples, 36 hBsAg positive and 9 randomly selected HBsAg negative were selected for HBV DNA extraction and PCR using established primers and protocols. The HBsAg prevalence was found to be 13.8% (51/369) with Oyo state having the highest (37.3%; N = 19/51) compared to other states. After amplification, HBs gene detection was 28.9%; (n = 13/45), HBc gene 33.3%; (n = 15/45), HB Pol gene 26.7%; (n = 12/45), and HBx gene 35.6%; (n = 16/45) and one OBI case (11.1%; n = 1/9). Considering the complications associated to HBV infections and the various changes being observed in the structure of the virus, more study is needed to enhance the diagnosis, manage- 230 ment, and control of hepatitis B.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":" ","pages":"467-477"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0