Production of anti-tetanus toxin IgY and study of its protective effects in a mouse model.

Q2 Health Professions

Journal of immunoassay & immunochemistry Pub Date : 2023-05-04 DOI:10.1080/15321819.2022.2138718

Mohammad Mohammadi, Mohammad Zangooei, Ebrahim Abbasi, Saeed Ebrahimi Fana, Mahdi Aminian

{"title":"Production of anti-tetanus toxin IgY and study of its protective effects in a mouse model.","authors":"Mohammad Mohammadi, Mohammad Zangooei, Ebrahim Abbasi, Saeed Ebrahimi Fana, Mahdi Aminian","doi":"10.1080/15321819.2022.2138718","DOIUrl":null,"url":null,"abstract":"Tetanus is an acute and often fatal infectious disease caused by Clostridium tetani. Tetanus toxin (TT) is responsible for spastic paralysis observed in tetanus. Anti-tetanus antibodies obtained from horses and humans are the most antitoxins used for tetanus treatment, although some clinical side effects and disadvantages have been reported in their application. The aim of this study is the production of anti-TT IgY and evaluation of its protective effects in a mouse model. Anti-TT IgY was purified from the egg yolk using PEG6000 precipitation and water dilution methods, and its purity was verified by SDS-PAGE. Finally, the potency of purified anti-TT IgY in neutralizing the lethal effects of TT was studied in vivo using a mouse model. PEG6000 precipitation method had better results. Animal studies showed that the purified IgY neutralized the toxic effects of 100 MLD of TT and multiple intravenous-dose injections of anti-TT IgY also had a continuous effect of TT neutralization. The purified anti-TT IgY was effective in neutralizing the lethal activity of TT in a mouse model. Our results suggested that IgY could be an alternative therapeutic source for the management of tetanus in the future.Abbreviations Anti-TT, Anti-tetanus toxin; ELISA, Enzyme-linked immunosorbent assay; IgY, Immunoglobulin Y; MLD, Minimum lethal dose; PBS, Phosphate buffer solution; PEG, Polyethylene glycol; SDS-PAGE, Sodium dodecyl sulfate polyacrylamide gel electrophoresis; TIG, Tetanus immune globulin; TT, Tetanus toxin; WD, Water dilution; RT, Room temperature.","PeriodicalId":15990,"journal":{"name":"Journal of immunoassay & immunochemistry","volume":"44 3","pages":"283-295"},"PeriodicalIF":0.0000,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of immunoassay & immunochemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/15321819.2022.2138718","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Health Professions","Score":null,"Total":0}

引用次数: 0

Abstract

Tetanus is an acute and often fatal infectious disease caused by Clostridium tetani. Tetanus toxin (TT) is responsible for spastic paralysis observed in tetanus. Anti-tetanus antibodies obtained from horses and humans are the most antitoxins used for tetanus treatment, although some clinical side effects and disadvantages have been reported in their application. The aim of this study is the production of anti-TT IgY and evaluation of its protective effects in a mouse model. Anti-TT IgY was purified from the egg yolk using PEG6000 precipitation and water dilution methods, and its purity was verified by SDS-PAGE. Finally, the potency of purified anti-TT IgY in neutralizing the lethal effects of TT was studied in vivo using a mouse model. PEG6000 precipitation method had better results. Animal studies showed that the purified IgY neutralized the toxic effects of 100 MLD of TT and multiple intravenous-dose injections of anti-TT IgY also had a continuous effect of TT neutralization. The purified anti-TT IgY was effective in neutralizing the lethal activity of TT in a mouse model. Our results suggested that IgY could be an alternative therapeutic source for the management of tetanus in the future.Abbreviations Anti-TT, Anti-tetanus toxin; ELISA, Enzyme-linked immunosorbent assay; IgY, Immunoglobulin Y; MLD, Minimum lethal dose; PBS, Phosphate buffer solution; PEG, Polyethylene glycol; SDS-PAGE, Sodium dodecyl sulfate polyacrylamide gel electrophoresis; TIG, Tetanus immune globulin; TT, Tetanus toxin; WD, Water dilution; RT, Room temperature.

查看原文本刊更多论文

抗破伤风毒素IgY的产生及其在小鼠模型中的保护作用研究。

破伤风是由破伤风梭菌引起的一种急性且往往致命的传染病。破伤风毒素(TT)是造成破伤风痉挛性麻痹的原因。从马和人身上获得的抗破伤风抗体是用于破伤风治疗的最常用的抗毒素，尽管在其应用中报道了一些临床副作用和缺点。本研究的目的是在小鼠模型中产生抗tt IgY并评估其保护作用。采用PEG6000沉淀和水稀释法从蛋黄中纯化抗tt IgY，并通过SDS-PAGE验证其纯度。最后，用小鼠模型研究了纯化的抗TT IgY在体内中和TT致死效应的效力。PEG6000沉淀法效果较好。动物实验表明，纯化的IgY可中和100 MLD的TT毒性作用，多次静脉注射抗TT IgY也具有持续中和TT的作用。纯化的抗TT IgY能有效中和TT在小鼠模型中的致死活性。我们的结果表明，IgY可能是未来治疗破伤风的一种替代治疗来源。Anti-TT，抗破伤风毒素;ELISA，酶联免疫吸附试验;IgY，免疫球蛋白Y;最小致死剂量;PBS，磷酸盐缓冲溶液;聚乙二醇;SDS-PAGE，十二烷基硫酸钠聚丙烯酰胺凝胶电泳;破伤风免疫球蛋白;TT，破伤风毒素;WD，水稀释;RT，室温。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of immunoassay & immunochemistry 医学-免疫学

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of Immunoassay & Immunochemistry is an international forum for rapid dissemination of research results and methodologies dealing with all aspects of immunoassay and immunochemistry, as well as selected aspects of immunology. They include receptor assay, enzyme-linked immunosorbent assay (ELISA) in all of its embodiments, ligand-based assays, biological markers of ligand-receptor interaction, in vivo and in vitro diagnostic reagents and techniques, diagnosis of AIDS, point-of-care testing, clinical immunology, antibody isolation and purification, and others.