Journal of global antimicrobial resistance最新文献

{"title":"Reliability of various antimicrobial susceptibility testing methods for piperacillin/tazobactam in challenging Escherichia coli isolates","authors":"Faruk Demirocak,&nbsp;Diana Langerak,&nbsp;Erlangga Yusuf","doi":"10.1016/j.jgar.2024.12.028","DOIUrl":"10.1016/j.jgar.2024.12.028","url":null,"abstract":"<div><h3>Objective</h3><div>Piperacillin/tazobactam antimicrobial susceptibility testing (AST) against Enterobacterales can be challenging. The aim of this study was to assess the reproducibility of various automated (VITEK 2) and nonautomated AST methods (broth microdilution (BMD), minimum inhibitory concentration (MIC) test strip, and disk diffusion) for piperacillin/tazobactam in ‘challenging’ <em>E. coli</em> isolates.</div></div><div><h3>Methods</h3><div>We performed 20 repeated ASTs for seven clinical <em>E. coli</em> isolates: Two resistant to piperacillin/tazobactam but susceptible to amoxicillin/clavulanic acid, four isolates with various β-lactamase coding genes (two <em>bla</em>_TEM-1, one <em>bla</em>_OXA-1, and one with plasmidal <em>bla</em>_ampC), and one isolate where VITEK 2 initially could not produce MIC measurements for piperacillin/tazobactam (i.e. no results generated).</div></div><div><h3>Results</h3><div>Upon repetition, the same MIC as the mode value (i.e. the most frequent MIC value of each AST method) was found between 21% and 87% (BMD), 46% and 100% (VITEK 2), and 48% and 100% (gradient test) of the repetitions. The range of essential agreement percentage (i.e. ±1 doubling dilution from this mode value) was 53–100% (BMD), 63–100% (VITEK 2), and 100% (gradient test). Percent categorical agreement (same susceptible of resistant category using EUCAST breakpoint v. 14.0) was 71–100% (BMD), 85–92% (VITEK 2), 76–100% (gradient test) and 100% (disk diffusion).</div></div><div><h3>Conclusions</h3><div><em>:</em> In conclusion, this study provides insight on the reliability of AST results for piperacillin/tazobactam in challenging <em>E. coli</em> isolates. While the results indicate that most methods are generally reproducible, certain isolates may present inconsistent MIC results.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"41 ","pages":"Pages 211-215"},"PeriodicalIF":3.7,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outbreak of carbapenem resistant Klebsiella pneumoniae in a neurorehabilitation unit: Genomic epidemiology reveals complex transmission pattern in a tertiary care hospital","authors":"Maria Grazia Silvotti ,&nbsp;Erika Scaltriti ,&nbsp;Luca Bolzoni ,&nbsp;Beatrice Zerbi ,&nbsp;Gabriella Tocci ,&nbsp;Andrea Zappavigna ,&nbsp;Gianfranco Lamberti ,&nbsp;Federico Donati ,&nbsp;Franco Federici ,&nbsp;Stefano Pongolini ,&nbsp;Giuliana Lo Cascio","doi":"10.1016/j.jgar.2025.01.001","DOIUrl":"10.1016/j.jgar.2025.01.001","url":null,"abstract":"<div><h3>Objectives</h3><div>Infections by carbapenem-resistant Enterobacterales (CRE) in hospitals represent a severe threat but little is known on outbreaks in rehabilitation wards caused by <em>Klebsiella pneumoniae</em> producing <em>Klebsiella pneumoniae</em> Carbapenemase (KPC-Kp). We report an outbreak by KPC-Kp, in a neurorehabilitation unit in Italy, analysed through whole-genome sequencing (WGS) for transmission routes reconstruction to improve management of KPC-Kp infections in rehabilitation units.</div></div><div><h3>Methods</h3><div>We investigated cases and KPC-Kp isolates collected from February to October 2022 from hospital surveillance. Carbapenem resistance was identified with disk diffusion and minimum inhibitory concentration tests, carbapenemase production through immunochromatographic lateral flow assays. All isolates were genotyped through WGS to highlight possible phylogenetic relationships. The most likely transmission networks of KPC-Kp were reconstructed with Bayesian discrete-time stochastic models.</div></div><div><h3>Results</h3><div>Nineteen patients were colonized by KPC-Kp. Two isolates belonged to sporadic sequence types (STs; ST348 and ST219) whereas 9 of 19 and 8 of 19 isolates belonged to ST307 and ST716, respectively. Among the ST307 isolates, we identified two genomically distinct clusters of five and two cases. All ST716 isolates were highly similar. Genotyping and the reconstruction of transmission networks of KPC-Kp based on genomic data identified seven independent introductions into the neurorehabilitation unit rather than a single outbreak as initially hypothesized before genomic investigation. Three of the seven introductions generated chains of secondary infections, whereas the remaining four remained single cases.</div></div><div><h3>Conclusions</h3><div>The outbreak root-causes were identified in temporary staff shortage and insufficient microbiological surveillance. Measures were adopted accordingly and the outbreak ended. The study highlights the critical role of genomic epidemiology in hospital outbreaks. © 2025 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"41 ","pages":"Pages 195-201"},"PeriodicalIF":3.7,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology and Resistance Profiles of Bacteria Isolated From Blood Samples in Septic Patients at Emergency Department Admission: A 6-Year Single Centre Retrospective Analysis From Northern Italy","authors":"Valeria Cento ,&nbsp;Sara Carloni ,&nbsp;Riccardo Sarti ,&nbsp;Linda Bussini ,&nbsp;Zian Asif ,&nbsp;Paola Morelli ,&nbsp;Francesco De Fazio ,&nbsp;Federica Maria Tordato ,&nbsp;Maddalena Casana ,&nbsp;Debora Mondatore ,&nbsp;Antonio Desai ,&nbsp;Elena Generali ,&nbsp;Nicola Pugliese ,&nbsp;Elena Costantini ,&nbsp;Massimo Vanoni ,&nbsp;Maurizio Cecconi ,&nbsp;Stefano Aliberti ,&nbsp;Giorgio Da Rin ,&nbsp;Erminia Casari ,&nbsp;Michele Bartoletti ,&nbsp;Antonio Voza","doi":"10.1016/j.jgar.2024.12.023","DOIUrl":"10.1016/j.jgar.2024.12.023","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate the microbiological and clinical heterogeneity of community-onset bloodstream infections (BSIs) and identify features to support targeted empirical antibiotic therapy in the Emergency Department (ED).</div></div><div><h3>Methods</h3><div>Clinical and microbiological data from 992 BSI cases (1,135 isolates) diagnosed within 24 h of ED admission at IRCCS Humanitas Research Hospital, Milan, Italy (January 2015–June 2022), were analysed. Drug resistance was interpreted using EUCAST-2023. Clinical features included age, sex, comorbidities (e.g., cancer, diabetes), infection source, presence of central venous catheters (CVC), ongoing therapies, and sepsis severity. Microbiological data included pathogen identification and antimicrobial susceptibility.</div></div><div><h3>Results</h3><div>Antibiotic-susceptible <em>Escherichia coli</em> (29.5%) was the most common isolate, including extended-spectrum beta-lactamase (ESBL)-producing strains (11.3%), followed by methicillin-susceptible <em>Staphylococcus aureus</em> (MSSA, 8.4%). BSIs due to <em>E. coli</em> were more frequent in patients &gt;60 years (43.9% vs. 27.3%, <em>P</em> &lt; 0.001) and associated with ESBL production (OR = 2.202, <em>P</em> = 0.031) and urosepsis (OR = 1.688, <em>P</em> = 0.006). Younger patients (≤60 years) had more <em>S. aureus</em>-associated BSIs (22.4% vs. 10.8%, <em>P</em> &lt; 0.001) and methicillin resistance (7.9% vs. 3.6%, <em>P</em> = 0.021). Carbapenem-resistant Enterobacterales were rare (2.1%-2.8%), predominantly involving <em>Klebsiella pneumoniae</em>. Onco-hematological patients had a lower multidrug-resistance prevalence (9.5% vs. 21.1%, <em>P</em> &lt; 0.001).</div></div><div><h3>Conclusions</h3><div>Community-onset BSIs demonstrated substantial prevalence of resistant pathogens, including ESBL and MRSA, emphasizing the need for robust surveillance systems. Age is a critical factor in guiding empirical antibiotic therapy in the ED.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"41 ","pages":"Pages 202-210"},"PeriodicalIF":3.7,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of I491F and V170F rpoB mutations associated with misdiagnosis of rifampicin resistance among patients with drug-susceptible tuberculosis treatment failure, Myanmar, 2022","authors":"Phyu Win Ei ,&nbsp;Mi Mi Htwe ,&nbsp;Myat Htut Nyunt ,&nbsp;Aye Su Mon ,&nbsp;Zaw Myint ,&nbsp;Wint Wint Nyunt ,&nbsp;Su Mon Win ,&nbsp;Sandar Aung ,&nbsp;Wai Myat Thwe ,&nbsp;Wah Wah Aung","doi":"10.1016/j.jgar.2024.12.026","DOIUrl":"10.1016/j.jgar.2024.12.026","url":null,"abstract":"<div><h3>Objective</h3><div>Detecting rifampicin (RIF) resistance is crucial in selecting tuberculosis (TB) treatment. Recently, several studies reported that I491F and V170F <em>rpo</em>B mutations were found with a varying prevalence. This study aimed to find out RIF resistance missed by routine diagnostic assays using next generation genome sequencing tool.</div></div><div><h3>Methods</h3><div>Sputum specimens from first-line TB treatment failed patients attending Tuberculosis Centers in Yangon Region during 2022 were cultured in solid media. Phenotypic drug susceptibility testing was conducted using Mycobacterial Growth Indicator Tube method. Whole genome or Deeplex-targeted next-generation sequencing was performed using Illumina Miseq. Mutation analysis was done by PhyResSE and SAM-TB online platforms.</div></div><div><h3>Results</h3><div>A total of 32 culture-positive isolates with DNA qualified for genome sequencing were included in the study. Those were diagnosed as rifampicin-susceptible by routine GeneXpert and line probe assays. RIF resistance-conferring mutations were found in 17/32 (53.1%) <em>Mycobacterium tuberculosis</em> isolates; 14 (43.7%) had mutations outside the RIF resistance determining region (I491F and V170F), two (6.3%) were S450L, mutation within RIF resistance determining region, and one isolate (3.1%) with interim resistance mutations S428T and S441A.</div></div><div><h3>Conclusion</h3><div>This study highlighted the presence of rifampicin-resistant TB strains missed by current diagnostic strategies, and are circulating as treatment-failed patients. This demonstrates a gap in current World Health Organization-endorsed algorithms for capturing all multidrug-resistant-TB strains.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"41 ","pages":"Pages 169-172"},"PeriodicalIF":3.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142965555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Simultaneous post-neurosurgical ventriculitis and bacteraemia by two different strains of KPC-producing K. pneumoniae successfully treated with meropenem/vaborbactam and high dose of Fosfomycin” [Journal of Global Antimicrobial Resistance 37 (2024) 86-90]","authors":"Lorenzo Volpicelli ,&nbsp;Sara Cairoli ,&nbsp;Dania Al Ismail ,&nbsp;Floriana Baisi ,&nbsp;Federica Sacco ,&nbsp;Bianca Maria Goffredo ,&nbsp;Mario Venditti ,&nbsp;Alessandra Oliva","doi":"10.1016/j.jgar.2024.12.009","DOIUrl":"10.1016/j.jgar.2024.12.009","url":null,"abstract":"","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"41 ","pages":"Page 20"},"PeriodicalIF":3.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"No genetic link between E. coli isolates carrying mcr-1 in bovines and humans in France","authors":"Marisa Haenni ,&nbsp;Pierre Châtre ,&nbsp;Racha Beyrouthy ,&nbsp;Antoine Drapeau ,&nbsp;Pauline François ,&nbsp;Jean-Yves Madec ,&nbsp;Richard Bonnet","doi":"10.1016/j.jgar.2024.12.021","DOIUrl":"10.1016/j.jgar.2024.12.021","url":null,"abstract":"<div><h3>Background</h3><div>Colistin is a last-line antibiotic used to treat severe human infections caused by carbapenemase-producing Gram-negative bacteria. In parallel, colistin has massively been used in the veterinary field so that <em>mcr-1</em>-positive <em>E. coli</em> have spread worldwide in livestock, potentially constituting a reservoir of colistin-resistant isolates that can be further transmitted to humans.</div></div><div><h3>Objectives</h3><div>In France, the <em>mcr-1</em> gene was frequently identified in <em>E. coli</em> of bovine origin. This genomic study assessed whether French human <em>mcr-1</em>-positive <em>E. coli</em> might originate or derive from the bovine reservoir.</div></div><div><h3>Material and methods</h3><div>Human (<em>n</em> = 24) and bovine (<em>n</em> = 127) isolates collected between 2011 and 2019 were included and colistin-resistance was confirmed by MICs. The detection of <em>mcr-1</em> was performed by PCR. Isolates were short-read whole-genome sequenced and a cgMLST-based phylogeny was constructed. The genetic support of <em>mcr-1</em> was identified using short-read sequences or Southern blots.</div></div><div><h3>Results</h3><div>The <em>mcr-1</em> gene was carried by a high diversity of genetic backgrounds, among which ST167 and ST10 were the most widespread. No clonally-related isolates between bovines and humans were observed. In bovines, <em>mcr-1</em> was identified on IncHI2 and IncX4 plasmids and increasingly on the chromosome, while it was also found on IncI2 and p0111 plasmids in humans.</div></div><div><h3>Conclusion</h3><div>Although similar STs (ST744 and ST88) and plasmid types (IncHI2, IncX4) carried <em>mcr-1</em>, no hypothesis of a transfer from bovines to humans could be supported by the data. Furthermore, the increasing chromosomal location of <em>mcr-1</em> over time may reflect an animal-specific evolutionary pathway deserving further investigation.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"41 ","pages":"Pages 111-116"},"PeriodicalIF":3.7,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-existence of two blaNDM-5 and blaOXA-181 on distinct plasmids in a carbapenem-resistant Klebsiella pneumoniae from a tertiary hospital, Tanzania","authors":"Lawrence Mapunda ,&nbsp;Anthon Mwingwa ,&nbsp;Doreen Kamori ,&nbsp;Happiness Kumburu ,&nbsp;Marco van Zwetselaar ,&nbsp;Bjorn Blomberg ,&nbsp;Joel Manyahi","doi":"10.1016/j.jgar.2024.12.011","DOIUrl":"10.1016/j.jgar.2024.12.011","url":null,"abstract":"<div><h3>Purpose</h3><div>To understand the mechanisms of carbapenem-resistant <em>Klebsiella pneumoniae</em> (CRKP) from Tanzania and characterize the genomes carrying the carbapenemase genes.</div></div><div><h3>Methods</h3><div>Clinical CRKP isolates were selected from ongoing antimicrobial resistance surveillance at Muhimbili National Hospital, Dar es Salaam, Tanzania. Whole-genome sequencing was performed utilizing Illumina and Nanopore platforms.</div></div><div><h3>Results</h3><div>A total of twelve CRKP were analyzed in this study. Six different multilocus sequence types were detected, six isolates were sequence type ST437 and one belonged to a novel sequence type, ST6258. Resistance to carbapenems was multifactorial with co-existence of <em>bla</em>_NDM-5 and <em>bla</em>_OXA-181 in six CRKP, and <em>bla</em>_NDM-5 and <em>bla</em>_OXA-232 in one isolate, and chromosomal mutation of ompK36 and ompK37 in all twelve isolates. All the CRKP carried genes conferring resistance to 3rd generation cephalosporins, penicillin, aminoglycosides, fosfomycin, trimethoprim-sulfamethoxazole, and quinolones. The hybrid assemblies of 001BS and 002PS2 revealed that they harbored seven and six different plasmids, respectively. The 001BS carried two <em>bla</em>_NDM-5 on distinct plasmids. The first <em>bla</em>_NDM-5 gene was carried on an IncFIB(K) plasmid; and the second <em>bla</em>_NDM-5 co-existed with <em>bla</em>_OXA-181 on the ColPK3-IncX3 plasmid. In contrast, in 002PS2 the <em>bla</em>_NDM-5 and <em>bla</em>_OXA-181 were carried on the IncFIB(K)-IncFII(K) and ColPK3-IncX3 plasmids, respectively. The genetic environment of the <em>bla</em>_NDM-5 gene on both plasmids was flanked by the same genetic core IS26–IS30–<em>bla</em>_NDM-5 –ble–<em>trp</em>F–<em>Dsb</em>D–ISCR1–<em>sul</em>1– QacE–IS3000.</div></div><div><h3>Conclusion</h3><div>Clonally related CRKP ST437 with multiple co-existing carbapenemase genes were detected for the first time at the tertiary hospital in Tanzania. The existence of this high-risk clone poses a great risk for further spread at our facility.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"41 ","pages":"Pages 173-180"},"PeriodicalIF":3.7,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and phylogenetic analysis of a NDM-1 producing ST152 Klebsiella pneumoniae isolated from a bloodstream infection in China","authors":"Renchi Fang ,&nbsp;Lingfang Di ,&nbsp;Xuebin Tian ,&nbsp;Yongfeng Bai","doi":"10.1016/j.jgar.2024.11.005","DOIUrl":"10.1016/j.jgar.2024.11.005","url":null,"abstract":"<div><h3>Purpose</h3><div>The aim of this study was to characterize the genomic structure of the first isolated ST152 <em>Klebsiella pneumoniae</em> strain carrying <em>bla</em>_NDM-1 in China.</div></div><div><h3>Methods</h3><div>Antimicrobial susceptibility was determined by broth microdilution method. Whole-genome sequencing was conducted using the Illumina HiSeq and Oxford Nanopore GridION X5 platforms, and the genomic features were analyzed using a range of bioinformatics tools.</div></div><div><h3>Results</h3><div>The strain HZKP1 demonstrated resistance to cefepime, ceftazidime, amoxicillin-clavulanic acid, amikacin, ciprofloxacin, meropenem, imipenem, and ertapenem, while showing sensitivity to tigecycline and colistin. It was classified as sequence type (ST) 152, K locus (KL) 105 and OC locus (OCL) O3b, respectively. The <em>bla</em>_NDM-1 gene was identified on the IncX3 plasmid pHZKP1-NDM (54,035 bp), located downstream of the IS<em>Kox3</em>-IS<em>3000</em>-IS<em>Aba125</em>-IS<em>5</em> segment and upstream of the <em>ble</em>_MBL-IS<em>26</em>-<em>bla</em>_SHV-69-IS<em>26</em> segment. Phylogenetic analysis indicated its closest relative to be a strain isolated from a wound swab sample in Tanzania in 2013, differing by 598 alleles.</div></div><div><h3>Conclusion</h3><div>We characterized genomic features of the first ST152 <em>K. pneumoniae</em> strain isolated in China. Our findings provide insights into the current status of resistance in new lineages within the region and enhance comprehension of <em>K. pneumoniae</em> carbapenem resistance transmission from a genetic structure perspective.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 34-36"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amikacin, in combination with cations, prevents growth of a carbapenem-resistant/multidrug-resistant Klebsiella pneumoniae strain isolated from a patient with COVID-19","authors":"Angel J. Magaña ,&nbsp;David Ngo ,&nbsp;Diego Faccone ,&nbsp;Sonia Gomez ,&nbsp;Alejandra Corso ,&nbsp;Fernando Pasteran ,&nbsp;María Soledad Ramirez ,&nbsp;Marcelo E. Tolmasky","doi":"10.1016/j.jgar.2024.11.007","DOIUrl":"10.1016/j.jgar.2024.11.007","url":null,"abstract":"","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 8-10"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation, management, and outcomes of New Delhi metallo-β-lactamase-producing Escherichia coli: A case series","authors":"Anita Shallal ,&nbsp;Michael P. Veve ,&nbsp;Rachel M. Kenney ,&nbsp;George Alangaden ,&nbsp;Geehan Suleyman","doi":"10.1016/j.jgar.2024.11.017","DOIUrl":"10.1016/j.jgar.2024.11.017","url":null,"abstract":"<div><h3>Objective</h3><div>New Delhi metallo-β-lactamase (NDM)-producing carbapenem-resistant <em>Enterobacterales</em> (CRE) is a globally growing threat. We sought to describe the microbiology, management and outcomes of patients with this infection at our facility.</div></div><div><h3>Methods</h3><div>This is a descriptive case series of patients with NDM-producing <em>Escherichia coli</em> isolated from culture in Detroit between July 2021 and February 2023. Demographics, risk factors, clinical characteristics, management and outcomes were described.</div></div><div><h3>Results</h3><div>Nine patients were included in the study. Most patients were female with a median age of 67 years. Hepatobiliary disease accounted for 90% of underlying conditions. Nearly all patients had prior antibiotic exposure and the most common specimen source was intra-abdominal fluid. Three patients were not treated due to colonisation; among those treated, the majority received trimethoprim-sulfamethoxazole. The median treatment duration and length of stay were 7 and 15.5 days, respectively. Six (67%) patients survived.</div></div><div><h3>Conclusions</h3><div>This report describes a large case series of NDM-producing <em>E. coli</em> infection. Patients with significant comorbidities remain at high risk for CRE infection. Antibiotic options for the treatment of NDM organisms are very limited; new and effective therapies are urgently needed.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 42-46"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}