Journal of global antimicrobial resistance最新文献

{"title":"Emerging resistance to novel β-lactam β-lactamase inhibitor combinations in Klebsiella pneumoniae bearing KPC variants","authors":"Mase Hamza ,&nbsp;German M. Traglia ,&nbsp;Lucia Maccari ,&nbsp;Sonia Gomez ,&nbsp;Maria Belen Sanz ,&nbsp;Usman Akhtar ,&nbsp;Vyanka Mezcord ,&nbsp;Jenny Escalante ,&nbsp;Alejandra Corso ,&nbsp;Cecilia Rodriguez ,&nbsp;Christopher R. Bethel ,&nbsp;Gauri G. Rao ,&nbsp;Marcelo E. Tolmasky ,&nbsp;David Paterson ,&nbsp;Robert A. Bonomo ,&nbsp;Fernando Pasteran ,&nbsp;Maria Soledad Ramirez","doi":"10.1016/j.jgar.2025.07.011","DOIUrl":"10.1016/j.jgar.2025.07.011","url":null,"abstract":"<div><h3>Objective</h3><div><em>Klebsiella pneumoniae</em> carbapenemase (KPC) variants, predominantly KPC-2 and KPC-3, are significant global resistance mechanisms, conferring resistance to many β-lactams, including carbapenems, while remaining susceptible to ceftazidime-avibactam (CZA). Recently, new KPC variants have developed resistance to CZA through mutations, insertions, or deletions in regions such as the Ω-loop, 240-loop (237–243 aa), and 270-loop (266–275 aa). This study investigated collateral resistance to cefiderocol (FDC) and cefepime/zidebactam (FPZ) in isolates with these mutations.</div></div><div><h3>Methods</h3><div>Fifteen clinical KPC-producing <em>Klebsiella</em> spp. isolates representing 15 distinct variants were analysed. Antimicrobial susceptibility testing determined the MICs for CZA, carbapenems, FDC, FPZ, and other antibiotics. Synergy between CZA and FDC was assessed. Whole-genome sequencing (WGS) was used to identify resistance-related mutations.</div></div><div><h3>Results</h3><div>CZA resistance was confirmed in 12/15 variants. Collateral resistance to FDC occurred in eight isolates, with five exhibiting spontaneous resistant subpopulations. Six FDC-resistant strains had mutations in the 270-loop (266–275 aa). FPZ resistance was seen in three KPC variants, especially those with mutations in the 270-loop, though many Ω-loop and 240-loop (237–243 aa) mutants remained susceptible. WGS of FDC-resistant subpopulations revealed additional mutations in <em>ompC, rpoC, dksA</em>, and <em>cirA</em>.</div></div><div><h3>Conclusions</h3><div>Emerging CZA-resistant KPC variants often exhibit collateral FDC resistance, with FPZ seen less frequently. Mutations in <em>bla</em>_KPC, <em>cirA</em>, and other genes contribute to resistance. Understanding these emerging resistant patterns linked with new KPC variants is crucial to inform therapeutic decisions, as emerging resistance may limit last-line treatment options in clinical settings.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 297-305"},"PeriodicalIF":3.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between pneumococcal serotypes and antimicrobial resistance: A systematic review and meta-analysis","authors":"Onyansaniba K. Ntim ,&nbsp;Aaron Awere-Duodu ,&nbsp;Samuel Addo Akwetey ,&nbsp;Fleischer C.N. Kotey ,&nbsp;Eric S. Donkor","doi":"10.1016/j.jgar.2025.07.008","DOIUrl":"10.1016/j.jgar.2025.07.008","url":null,"abstract":"<div><h3>Background</h3><div>The diversity of pneumococcal serotypes poses significant challenges for both vaccination and treatment. Clinical management of pneumococcal infections is further complicated by the fact that individual serotypes exhibit distinct antimicrobial resistance patterns. This study aims to comprehensively estimate the antimicrobial resistance rates of pneumococcal serotypes.</div></div><div><h3>Methods</h3><div>A comprehensive search was conducted in PubMed, Scopus, Web of Science, and ScienceDirect. A random-effect meta-analysis was used to pool the number of isolates resistant to a particular antimicrobial per total number of isolates tested for each pneumococcal serotype.</div></div><div><h3>Results</h3><div>Ninety studies were included in the analysis. The study identified 66 pneumococcal serotypes, with vaccine serotypes such as 19A, 19F, 23F, 6B, 14, 6A, 3, and 9V being the most frequently reported. Non-vaccine serotypes like 15A, 6C, 23A, 23B, 15B, and 35B were also prevalent. Most serotypes were associated with pneumococcal diseases. Serotypes exhibited varying and high resistance to cefuroxime, co-trimoxazole, tetracycline, macrolides, and clindamycin. The highest multidrug resistance was observed in serotypes 23F (63.34%, 95% CI [25.77; 94.31]), 15C (61.04%, 95% CI [0.74; 100.00]), 23A (57.28%, 95% CI [27.60; 84.58]), 19F (55.95%, 95% CI [30.26; 80.22]), 19A (54.07%, 95% CI [32.60; 74.90]), 15B (47.10%, 95% CI [9.66; 86.41]), 24F (45.77%, 95% CI [3.79; 91.31]), 15A (44.28%, 95% CI [29.58; 59.26]), and 6B (43.79%, 95% CI [28.48; 59.12]).</div></div><div><h3>Conclusions</h3><div>This study highlights significant antimicrobial resistance among both vaccine (19A, 19F, 23F, 14, 6A, and 6B) and non-vaccine types (15A, 6C, 23A, 20, 15C, and 35B). Our findings emphasize the need for effective surveillance and targeted interventions to fight these resistant pneumococcal serotypes.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"45 ","pages":"Pages 52-67"},"PeriodicalIF":3.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salmonella Minnesota sequence type 548 harbouring a type 2 IncC megaplasmid of antimicrobial resistance and virulence (pESM) infecting a companion animal","authors":"Luciana Sartori ,&nbsp;João Pedro Rueda Furlan ,&nbsp;Fábio P. Sellera ,&nbsp;Fernanda B. Barbosa ,&nbsp;Bruna Fuga ,&nbsp;Gregory Batista Melocco ,&nbsp;Karine Sousa Dantas ,&nbsp;Thais Martins-Gonçalves ,&nbsp;Ronan Adler Tavella ,&nbsp;Ana Cristina Gales ,&nbsp;Nilton Lincopan ,&nbsp;Terezinha Knöbl","doi":"10.1016/j.jgar.2025.07.007","DOIUrl":"10.1016/j.jgar.2025.07.007","url":null,"abstract":"<div><h3>Objectives</h3><div>This study reports the identification and genomic characteristics of <em>Salmonella</em> strains isolated from a blood sample of a 9-year-old male Persian cat with a systemic infection (strain M885) and from a pleural effusion sample of a 9-year-old male Bulldog (strain T886) in Brazil.</div></div><div><h3>Methods</h3><div>Genomic DNA was sequenced using the Illumina NextSeq platform, <em>de novo</em> assembled by SPAdes version 3.15.2, and annotated by the RAST server. Serovars, sequence types (STs), antimicrobial resistance genes, plasmid replicons, <em>Salmonella</em> pathogenicity islands, and single nucleotide polymorphism (SNP) analysis were accomplished by bioinformatic tools.</div></div><div><h3>Results</h3><div>Strains M885 and T886 belonged to the serovars Sandiego (ST126) and Minnesota (ST548), respectively. Strain T886 carried <em>bla</em>_CMY-2 and <em>qnrB19</em> onto IncC2 and Col(pHAD28) plasmids, respectively. The <em>bla</em>_CMY-2-bearing IncC2 plasmid also harboured mercury tolerance genes and the yersiniabactin virulence gene cluster, being classified as a type 2 IncC megaplasmid of antimicrobial resistance and virulence (plasmid for emergent <em>S</em>. Minnesota [pESM]). SNP-based analysis revealed clonal relatedness between the T886 strain and a CMY-2-producing <em>S</em>. Minnesota ST548 previously isolated from chicken sausage in Brazil, supporting a common ancestral origin.</div></div><div><h3>Conclusions</h3><div>This study underscores the importance of monitoring <em>S. enterica</em> as the causative agent of extra-intestinal infections in small animal medicine. Therefore, the transmission dynamics and effective strategies for managing infections produced by multidrug-resistant clones adapted to the human-animal-environmental interface warrant further investigation.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 262-264"},"PeriodicalIF":3.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico design of sgRNAs with knockout potential for the adeB gene of the AdeABC efflux pump of Acinetobacter baumannii","authors":"José Miguel Benigno Delgado Ruitón ,&nbsp;Pedro Jorge Chimoy Effio ,&nbsp;Guillermo Uceda Campos ,&nbsp;Edgar Enrique Llontop Cornejo ,&nbsp;Erick Giancarlo Suclupe Farro","doi":"10.1016/j.jgar.2025.07.001","DOIUrl":"10.1016/j.jgar.2025.07.001","url":null,"abstract":"<div><h3>Objectives</h3><div>The efflux pump AdeABC, regulated by the AdeRS operon, is a key factor in multidrug resistance in <em>Acinetobacter baumannii</em>. The <em>adeB</em> gene encodes the transmembrane transporter protein, making it a promising target for therapeutic gene disruption.</div></div><div><h3>Methods</h3><div>We designed and analyzed single guide RNAs (sgRNAs) targeting the adeB gene using CHOPCHOP. Conservation analysis was conducted with BLAST and multiple alignment tools. Potential off-target effects were assessed using Cas-OFFinder. The thermodynamic stability and accessibility of top-ranked sgRNAs were evaluated using Mfold. Structural and functional domain mapping was performed based on the 3D model of AdeB protein. Phylogenetic analyses of <em>adeB</em> alleles were carried out using MAFFT and iTOL.</div></div><div><h3>Results</h3><div>Among 49 designed sgRNAs, five candidates met stringent criteria for cleavage efficiency, GC content, conservation across strains, and low off-target potential. Secondary structure analysis confirmed their stability, and domain mapping showed that the sgRNAs targeted critical regions of AdeB, including transmembrane helices and substrate-binding sites. Phylogenetic clustering confirmed high sequence conservation in clinical strains.</div></div><div><h3>Conclusion</h3><div>This study presents three validated sgRNAs with knockout potential against the <em>adeB</em> gene of <em>A. baumannii</em>. These sgRNAs may serve as effective molecular tools to disrupt the AdeABC pump and combat efflux-mediated antimicrobial resistance. Further in vitro validation is proposed to assess their functional impact.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 272-280"},"PeriodicalIF":3.2,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the tet(M)-bearing transposon Tn7125 of Escherichia coli strain A13 isolated from an intensive pig farm located in Henan province, China","authors":"Yingying Liu ,&nbsp;Hanmeng Ren ,&nbsp;Jiaqi Zeng ,&nbsp;Xinyue Zhang ,&nbsp;Mingcheng Wang ,&nbsp;Zhu Qiao ,&nbsp;Yan Ma ,&nbsp;Chaoying Liu ,&nbsp;Huili Xia ,&nbsp;Gongzheng Hu ,&nbsp;Enzhong Li","doi":"10.1016/j.jgar.2025.06.022","DOIUrl":"10.1016/j.jgar.2025.06.022","url":null,"abstract":"<div><h3>Background</h3><div>Transposons carrying <em>tet</em>(M) in Gram-positive bacteria have been reported extensively, while there is a paucity of data on the transmission characteristics of <em>tet</em>(M) in Gram-negative bacteria. Therefore, the aim of this study was to investigate the genetic characteristics of the <em>tet</em>(M)-bearing transposon Tn<em>7125</em>, and to clarify the transmission mechanism of the plasmids pTA13-1 and pTA13-3 in <em>Escherichia coli</em> strain A13.</div></div><div><h3>Methods</h3><div>Plasmids from strain A13 and a corresponding transconjugant were determined by whole genome sequencing and analyzed using bioinformatics tools. The plasmids pTA13-1 and pTA13-3 of the transconjugant TA13 were characterized by S1-pulse-field gel electrophoresis, Southern hybridization, stability experiments, and direct competition assays.</div></div><div><h3>Results</h3><div>The conjugated IncF2:A6:B20 plasmid pTA13-1 co-transferred with the 41-kb plasmid pTA13-3, which carried no resistance genes; plasmid pTA13-2, which harbored the replication initiator PO111; and the IncX4 plasmid pTA13-4, which harbored the antibiotic resistance gene <em>mcr-1.</em> The novel IS<em>26</em>-bracked composite transposon Tn<em>7125</em> was located on plasmid pTA13-1, which mainly consists of three resistance modules: IS<em>26</em>-ctp-lp-<em>tet</em>(M)-hp-IS<em>406</em>tnp, <em>qac</em>-<em>aadA1</em>-<em>cmlA1</em>-<em>aadA2</em>-DUF1010-<em>dfrA12</em>, and <em>∆</em>IS<em>VSa3-VirD-floR-LysR-</em>IS<em>VSa3.</em> The plasmid pTA13-1 was highly stable in <em>E. coli</em> strain J53 with no fitness cost to the host or disadvantage in growth competition.</div></div><div><h3>Conclusion</h3><div>Evolution of co-integrated transposons, such as Tn<em>7125</em>, may convey antibiotic resistance to a wide spectrum of hosts via the plasmids pTA13-1 and pTA13-3, which acts as an adaptable and mobile multidrug resistance reservoir to accelerate dissemination of other genes by co-selection, thereby posing a potentially serious barrier to clinical treatment regimens.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 244-250"},"PeriodicalIF":3.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing access to antimicrobial resistance diagnostics for the marginalised: Challenges and opportunities of point-of-care technologies","authors":"Vinay Modgil ,&nbsp;Sundeep Sahay ,&nbsp;Neelam Taneja ,&nbsp;Arunima Mukherjee ,&nbsp;Neha Joshi ,&nbsp;Arun Mitra ,&nbsp;Biju Soman ,&nbsp;Ravikant Singh ,&nbsp;Oshin Sinha ,&nbsp;Rashmi Surial","doi":"10.1016/j.jgar.2025.06.023","DOIUrl":"10.1016/j.jgar.2025.06.023","url":null,"abstract":"<div><h3>Objective</h3><div>Inadequate access to antimicrobial resistance (AMR) diagnostics remains a critical challenge, particularly in low- and middle-income countries (LMICs). Despite various global policy commitments, translating these policies into practice is restricted by socio-economic barriers, technological limitations, and infrastructure gaps.</div></div><div><h3>Methods</h3><div>Based on ongoing empirical work in India under EquityAMR, diagnostic inequities were examined in two contrasting states.</div></div><div><h3>Results</h3><div>The spatial analysis demonstrated disproportionate diagnostic services, due to urban-rural and hilly-plains regions. Further laboratory assessments across 14 health institutions in these two states highlighted critical gaps in testing capacity, quality control, equipment, and data management. In addition, the potential of existing point-of-care technologies in expanding access were examined based on WHO criteria of clinical preparedness, scalability, and sustainability, although none were considered suitable for all three criteria.</div></div><div><h3>Conclusions</h3><div>The study concluded that by arguing in the current scenario, traditional culture-based microbiology facilities can be established in sub-district facilities with some success in expanding access.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 281-286"},"PeriodicalIF":3.2,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A silent sulfonamide-resistant microbial world: The curious case of missing DHPS in candidate phyla radiation","authors":"Riyad Razzouk ,&nbsp;Adel Azour ,&nbsp;Jean-Marc Rolain ,&nbsp;Fadi Bittar","doi":"10.1016/j.jgar.2025.06.024","DOIUrl":"10.1016/j.jgar.2025.06.024","url":null,"abstract":"<div><div>Candidate phyla radiation (CPR) microbes exhibit minimal genomes, episymbiotic/parasitic lifestyles, and metabolic dependencies on host cells. In this study, we look for the presence of dihydropteroate synthase (DHPS) and dihydrofolate reductase, key folate biosynthesis enzymes targeted by sulfonamides and trimethoprim, respectively. Using bioinformatic computational methods, analysis of 12 535 (complete and non-complete) CPR genomes revealed the presence of dihydrofolate reductase enzyme in 54% of them, while DHPS was detected in only 6%, suggesting an inherent absence of the <em>folP</em> gene (corresponding to the DHPS). Structural and functional validation confirmed the lack of DHPS activity. These findings indicate an intrinsic resistance to sulfonamides in CPR microbes, raising questions about their adaptation and persistence in antibiotic-rich environments.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 241-243"},"PeriodicalIF":3.7,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic characterization of carbapenem-resistant Pseudomonas aeruginosa from ICU admission screening in Hanoi, Vietnam, 2023","authors":"Lisa Göpel ,&nbsp;Truong Nhat My ,&nbsp;Kaan Kocer ,&nbsp;Thi Anh Mai Pham ,&nbsp;Le Thi Kieu Linh ,&nbsp;Bui Tien Sy ,&nbsp;Leo Huber ,&nbsp;Tran Thanh Tung ,&nbsp;Nguyen Trong The ,&nbsp;Le Huu Song ,&nbsp;Sébastien Boutin ,&nbsp;Thirumalaisamy P. Velavan ,&nbsp;Dennis Nurjadi","doi":"10.1016/j.jgar.2025.07.002","DOIUrl":"10.1016/j.jgar.2025.07.002","url":null,"abstract":"<div><h3>Objective</h3><div>Vietnam is among the countries most affected by antimicrobial resistance in the Asia-Pacific. While multidrug-resistant Enterobacterales have been extensively studied, genomic data on multidrug-resistant <em>Pseudomonas aeruginosa</em> in Vietnam remains scarce. To address this, we characterized 20 carbapenem-resistant <em>P. aeruginosa</em> (CRPA) isolates from rectal colonization of ICU patients.</div></div><div><h3>Methods</h3><div>Screening for CRPA was conducted using a selective chromogenic medium (mSuperCARBA). Species identification was achieved through MALDI-TOF mass spectrometry, while antimicrobial susceptibility testing was performed using the Vitek®2 system and broth microdilution. Whole-genome sequencing was performed using the Illumina NextSeq platform.</div></div><div><h3>Results</h3><div>Twenty CRPA isolates were collected from rectal swabs of 691 patients admitted to the ICUs of the 108 Military Central Hospital in Hanoi, Vietnam, between 1 July 2023 and 31 October 2023. The predominant multilocus sequence type was ST308, accounting for 50% (10/20) of the isolates. Notably, 70% (14/20) of the CRPA isolates harboured genes encoding metallo-β-lactamases (MBL), with <em>bla</em>_NDM-1 being the most prevalent (86%, 12/14), followed by <em>bla</em>_IMP-26 (14%, 2/14). Low susceptibility was observed for ceftazidime-avibactam (15%, 3/20) and ceftolozane-tazobactam (10%, 2/20), while cefiderocol resistance was observed in 50% (10/20) of isolates. Colistin demonstrated the most favourable susceptibility profile, with 90% (18/20) of isolates remaining susceptible.</div></div><div><h3>Conclusions</h3><div>A significant proportion of CRPA isolates in our study were MBL producers, with high levels of resistance to novel β-lactams and β-lactam/β-lactamase inhibitor combinations. These findings underscore the urgent need for effective infection prevention and control strategies to mitigate the further spread of MBL-producing CRPA.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 265-271"},"PeriodicalIF":3.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cefiderocol susceptibility of Stenotrophomonas maltophilia species complex and carbapenem-resistant Pseudomonas aeruginosa isolates from blood cultures at a university hospital in Tokyo, Japan","authors":"Wataru Aoki ,&nbsp;Yoshifumi Uwamino ,&nbsp;Nagi Niida ,&nbsp;Hiroaki Kubota ,&nbsp;Yuka Kamoshita ,&nbsp;Rika Inose ,&nbsp;Mika Nagata ,&nbsp;Osamu Ishihara ,&nbsp;Shunsuke Uno ,&nbsp;Ayumi Yoshifuji ,&nbsp;Ho Namkoong ,&nbsp;Naoki Hasegawa ,&nbsp;Hiromichi Matsushita","doi":"10.1016/j.jgar.2025.07.004","DOIUrl":"10.1016/j.jgar.2025.07.004","url":null,"abstract":"<div><h3>Objective</h3><div>Cefiderocol, a siderophore cephalosporin, has shown promise against carbapenem-resistant strains such as <em>Pseudomonas aeruginosa</em> and <em>Stenotrophomonas maltophilia</em> species complex. Despite its recent approval in Japan, susceptibility data remain limited. Therefore, this study evaluated the cefiderocol susceptibility of carbapenem-resistant <em>P. aeruginosa</em> and <em>S. maltophilia</em> species complex strains isolated from blood cultures to determine whether cefiderocol can be used as an empirical treatment option.</div></div><div><h3>Methods</h3><div>Carbapenem-resistant <em>P. aeruginosa</em> and <em>S. maltophilia</em> species complex strains isolated from blood cultures at a university hospital in Japan were included. Minimum inhibitory concentrations (MICs) and inhibition zone diameters were assessed using broth microdilution and disk diffusion methods, respectively. Whole-genome sequencing was conducted to elucidate the genetic backgrounds of the clinical isolates.</div></div><div><h3>Results</h3><div>The MIC₅₀ and MIC₉₀ values for cefiderocol in 65 carbapenem-resistant <em>P. aeruginosa</em> strains were 0.12 and 0.25 µg/mL, and 0.06 and 0.25 µg/mL for 51 <em>S. maltophilia</em> species complex strains, respectively. One <em>S. maltophilia</em> species complex strain exhibited non-susceptibility, with a MIC of 2 µg/mL in broth microdilution, while disk diffusion methods demonstrated susceptibility. A significant negative correlation was observed between MIC values and inhibition zone diameters. Whole-genome sequencing revealed that a limited number of <em>P. aeruginosa</em> strains possessed carbapenemase genes.</div></div><div><h3>Conclusions</h3><div>The findings of this study support the efficacy of cefiderocol against carbapenem-resistant <em>P. aeruginosa</em> and <em>S. maltophilia</em> species complex, suggesting its potential as an empirical treatment option for bloodstream infection patients in Japan. Continued surveillance is advised to monitor resistance trends with increased cefiderocol use.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 251-255"},"PeriodicalIF":3.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of Citrobacter freundii and Enterobacter cloacae complex isolates co-carrying blaNDM-1 and mcr-9 from three hospitals","authors":"Aneta Kovarova ,&nbsp;Merit Amadasun ,&nbsp;Brigid Hooban ,&nbsp;Francesca McDonagh ,&nbsp;Anna Tumeo ,&nbsp;Kate Ryan ,&nbsp;Christina Clarke ,&nbsp;Martin Cormican ,&nbsp;Georgios Miliotis","doi":"10.1016/j.jgar.2025.07.003","DOIUrl":"10.1016/j.jgar.2025.07.003","url":null,"abstract":"<div><h3>Objectives</h3><div>Antimicrobial resistance (AMR) is a global health concern related to antimicrobial use and the subsequent emergence of resistant organisms, including carbapenemase-producing Enterobacterales (CPE). CPE isolate co-carrying <em>bla</em>_NDM-1 and <em>mcr-9.1</em> have been scarcely reported internationally. The identification of 20 such isolates, including 16 of one species, within a group of three hospitals in one region indicated potential dissemination within and between healthcare facilities.</div></div><div><h3>Methods</h3><div>Twenty isolates were pseudo-anonymised and identified via MALDI-ToF MS. Antimicrobial susceptibility testing was performed by disc diffusion, and Minimal Inhibition Concentration for colistin was carried out using the UMIC system. Short-read sequencing was conducted using the Illumina MiSeq platform, and genomic analysis identified antimicrobial resistance genes, virulence factors and plasmid contigs. Taxonomic classification of draft genomes was bioinformatically assessed using Kraken2.</div></div><div><h3>Results</h3><div>This collection comprised of <em>Enterobacter hormaechei</em> (<em>n</em> = 16), <em>Citrobacter freundii</em> (<em>n</em> = 3) and <em>Enterobacter cloacae</em> (<em>n</em> = 1) sourced from patient rectal swabs collected during routine screening (<em>n</em> = 13) or from healthcare-associated environmental sites (<em>n</em> = 7). The <em>E. hormaechei</em> isolates included four different ST types with one unassigned ST. Contig-based plasmid analysis identified 17 plasmid replicon types among the isolates. IncHI2A, IncHI2, and pKPC<img>CAV1321_1 were detected in all isolates. Linked <em>bla</em>_NDM-1 and <em>mcr-9.1</em> gene spread in hospitals likely occurred via plasmid-mediated transfer rather than spread of <em>E. hormaechei.</em></div></div><div><h3>Conclusions</h3><div>This study represents the first documented instance of <em>bla</em>_NDM-1/<em>mcr-9.1</em> co-occurrence in Europe to date. It highlights the increasing public health threat posed by antimicrobial resistance and underscores the importance of genomic surveillance and clinical screening.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 226-233"},"PeriodicalIF":3.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144600688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}