Journal of global antimicrobial resistance最新文献

{"title":"Antimicrobial susceptibilities of oral Neisseria from men on HIV pre-exposure prophylaxis in Hanoi, Vietnam","authors":"Huan Vinh Dong ,&nbsp;Paul Adamson ,&nbsp;Dieu Hoa Pham ,&nbsp;Quynh Hoa Pham ,&nbsp;Hai Ha Long Le ,&nbsp;Loc Quang Pham ,&nbsp;Hao Thi Minh Bui ,&nbsp;Giang Minh Le ,&nbsp;Jeffrey D. Klausner","doi":"10.1016/j.jgar.2024.11.008","DOIUrl":"10.1016/j.jgar.2024.11.008","url":null,"abstract":"<div><h3>Background</h3><div>Antimicrobial resistance (AMR) in <em>Neisseria gonorrhoeae</em> (<em>N. gonorrhoeae</em>) is an urgent global health concern. Commensal <em>Neisseria</em> species in the oropharynx are an important reservoir of AMR genes that are transferred to <em>N. gonorrhoeae</em>, yet few data about AMR among commensal <em>Neisseria</em> in populations at risk for AMR exist.</div></div><div><h3>Methods</h3><div>From May 2022 to December 2023, men in an HIV pre-exposure prophylaxis program in Hanoi, Vietnam, were recruited for this study. Participants self-collected oral specimens using phosphate buffer solution, for culture on LB agar media containing sucrose, vancomycin, and trimethoprim (LBVT.SNR). Oxidase-positive Gram-negative diplococci were identified using the Remel RapID NH system. Minimum inhibitory concentrations (MICs) to azithromycin, ceftriaxone, cefixime, and doxycycline were determined using Etests.</div></div><div><h3>Results</h3><div>There were 42 male participants, the median age was 26 years and 29% (<em>n</em> = 12) reported using antibiotics in the past 6 months. In total, 48 <em>Neisseria</em> isolates were recovered; <em>N. sicca/subflava</em> was the most common species (50%; <em>n</em> = 24), followed by <em>N. mucosa</em> (38%; <em>n</em> = 18). For azithromycin, 85% (<em>n</em> = 41) of isolates were resistant with MICs ≥ 1 ug/mL, including 25% (<em>n</em> = 12) with high-level resistance (MICs ≥ 256 ug/mL of which 67% (8/12) were <em>N. mucosa</em>. Among non-gonococcal <em>Neisseria</em> isolates, the prevalence of resistance was 6% (<em>n</em> = 3) for ceftriaxone, 6% (<em>n</em> = 3) for cefixime, and 54% (<em>n</em> = 26) for doxycycline; the most non-susceptible isolates were <em>N. mucosa.</em></div></div><div><h3>Conclusions</h3><div>A High frequency of azithromycin resistance, moderate doxycycline resistance, and low cephalosporin resistance was found in oropharyngeal <em>Neisseria</em> isolates from men who have sex with men (MSM) in a pre-exposure prophylaxis (PrEP) program in Hanoi, Vietnam. <em>N. mucosa</em> was over-represented among resistant isolates.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 11-14"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142755142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clonal dissemination of an NDM-2-producing Acinetobacter baumannii ST103 clone resulting in an outbreak in an intensive care unit of a Lebanese tertiary care hospital","authors":"Tania Nawfal Dagher ,&nbsp;Linda Hadjadj ,&nbsp;Fadi Bittar ,&nbsp;Fadi Fenianos ,&nbsp;Elias Abdo ,&nbsp;Jean-Marc Rolain ,&nbsp;Charbel Al-Bayssari","doi":"10.1016/j.jgar.2024.11.016","DOIUrl":"10.1016/j.jgar.2024.11.016","url":null,"abstract":"<div><h3>Objective</h3><div>Multidrug-resistant bacteria, including carbapenem-resistant <em>Acinetobacter baumannii</em> (CRAB), are considered a major public health threat, particularly those which are responsible for nosocomial infections. This study aimed to investigate the molecular mechanism of carbapenem resistance and the clonal relationship of CRAB isolates of a probable outbreak in the intensive care unit (ICU) of Saydet Zgharta University Medical Center, in north Lebanon.</div></div><div><h3>Methods</h3><div>Thirty-two non-duplicate CRAB isolates were collected from patients hospitalised in the ICU. Antibiotic susceptibility testing was carried out using the disk diffusion method and carbapenemase-encoding genes were searched for using standard polymerase chain reaction. Epidemiological relatedness was studied using multilocus sequence typing.</div></div><div><h3>Results</h3><div>Polymerase chain reaction and multilocus sequence typing results suggested the presence of two different periods: period 1 (November 2018 to February 2019), where 15 CRAB isolates were collected harbouring the <em>bla</em>_OXA-23 and <em>bla</em>_OXA-24 genes mainly and belonging to several clones; and period 2 (March to May 2019), considered an outbreak period where 17 carbapenem-resistant isolates were isolated, harbouring mostly the <em>bla</em>_NDM-2 gene, never previously described in Lebanon and belonging to ST103. Infection control measures implemented in Saydet Zgharta University Medical Center successfully eradicated the NDM-2-producing CRAB ST103 clone, thus putting an end to this outbreak in the ICU department.</div></div><div><h3>Conclusions</h3><div>This study showed that infection control measures and adequate identification of NDM-producing <em>A. baumannii</em> remain a powerful tool to limit the spread of such resistant micro-organisms.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 66-71"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of ST15 carbapenem-resistant Klebsiella pneumoniae isolated in a single patient","authors":"Yongjin Hu ,&nbsp;Rong Tang ,&nbsp;Shanshan Jin ,&nbsp;Jiahao Guan ,&nbsp;Xiaoxiao Meng ,&nbsp;Zengpeijie Dan ,&nbsp;Ruilan Wang ,&nbsp;Hong-Yu Ou ,&nbsp;Jian Lu","doi":"10.1016/j.jgar.2024.11.003","DOIUrl":"10.1016/j.jgar.2024.11.003","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;The carbapenem-resistant &lt;em&gt;Klebsiella pneumoniae&lt;/em&gt; (CRKP) poses a serious threat to antibiotic applicability and public health. During treatment, &lt;em&gt;K. pneumoniae&lt;/em&gt; (KP) frequently exhibits shifts in drug-resistant phenotypes, complicating clinical treatment as it transitions from sensitivity to resistance. In this study, we analysed the clinical and molecular characteristics of drug resistance changes in KP strains isolated from a single patient, and the potential mechanisms underlying these resistance changes.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;Antimicrobial susceptibility test and string test were conducted to evaluate the resistant and virulent characterization of the strains. Pulsed-field gel electrophoresis (PFGE) was used to investigate the homology relationship between the strains. The whole genome sequencing and phylogenetic analysis of 9 representative isolates was also performed. The transfer ability of the drug-resistant plasmid was studied by plasmid conjugation experiment. The transconjugants were verified by polymerase chain reaction amplification of specific genes, antimicrobial susceptibility test and PFGE.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;Our results revealed that 9 KP strains, isolated from the same patient, exhibited ‘resistance-sensitivity-resistance-sensitivity’ alternately to carbapenems. The differences in DNA fingerprint bands among the nine KP isolates were ≤3, which can be classified as the same PFGE type. Phylogenetic analysis showed that these 9 strains constituted a distinct branch within the phylogenetic tree. All nine KP strains belonged to the ST15-KL19 clone. Six of the strains were classified as CRKP, all of which carried 11 drug resistance genes: &lt;em&gt;oqxB, oqxA, fosA6, aac(3)-lld, bla&lt;/em&gt;&lt;sub&gt;SHV-28&lt;/sub&gt;, &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;KPC-2&lt;/sub&gt;, &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;OXA-1&lt;/sub&gt;, &lt;em&gt;mph(A), tet(A), catB3&lt;/em&gt; and &lt;em&gt;aac(6′)-lb-cr&lt;/em&gt;, mediating drug resistance to quinolones, fosfomycin, aminoglycosides, β-lactam, carbapenems, macrolides and chloramphenicol, belonging to multi-drug resistant bacteria. The carbapenem-resistant plasmid p2-KP3762–1 was found to transfer within species, from CRKP to hypervirulent KPRJF293HA, carbapenem-sensitive KP KP3657 and &lt;em&gt;Escherichia coli&lt;/em&gt; C600 at a frequency of (1.19 ± 1.58) ×10&lt;sup&gt;−6&lt;/sup&gt;, (1.09 ± 1.38) ×10&lt;sup&gt;−7&lt;/sup&gt; and (10.9 ± 9.53) ×10&lt;sup&gt;−6&lt;/sup&gt; respectively, resulting in the dissemination of carbapenem resistance genes.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;div&gt;In this study, KP strains isolated from a single patient exhibited an alternating phenotype of resistant-sensitive-resistant-sensitive to carbapenems. The 9 KP isolates share a high degree of genetic similarity. The plasmid p2-KP3762–1, harbouring the carbapenem resistance gene &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;KPC-2&lt;/sub&gt;, may undergo inter-strain and inter-clone transfer via conjugation in the patient during treatment. Furthermore, our findings suggest that the pathogens in this pati","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 72-80"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ISAC Information Page","authors":"","doi":"10.1016/S2213-7165(25)00027-X","DOIUrl":"10.1016/S2213-7165(25)00027-X","url":null,"abstract":"","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Page i"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143269903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive analysis of the epidemiological and genomic characteristics of global Serratia Marcescens","authors":"Wenqin Chen ,&nbsp;Zheqian Li ,&nbsp;Jie Zheng ,&nbsp;Danwei Wang ,&nbsp;Shuo Gao ,&nbsp;Wanqing Zhou ,&nbsp;Han Shen ,&nbsp;Yan Zhang ,&nbsp;Xiaoli Cao","doi":"10.1016/j.jgar.2024.11.013","DOIUrl":"10.1016/j.jgar.2024.11.013","url":null,"abstract":"<div><h3>Background</h3><div><em>Serratia marcescens</em> outbreaks present significant challenges in clinical treatment, necessitating a deeper understanding of its epidemiological and genomic traits.</div></div><div><h3>Objective</h3><div>To analyse the epidemiological and genomic characteristics of <em>S. marcescen</em>s at a global scale.</div></div><div><h3>Methods</h3><div>High-quality genomes of <em>S. marcescens</em> were retrieved from NCBI and annotated using Prodigal. Antibiotic resistance genes (ARGs) were identified via Blastn, sequence types (STs) were determined with a proprietary tool, and phylogenetic analysis was conducted to explore evolutionary relationships.</div></div><div><h3>Results</h3><div>The study analysed genomes from 33 countries, with major contributions from the USA (27.8%), UK (15.3%), Italy (14.7%), and Japan (10.7%). Human clinical samples accounted for 73.5% of the isolates, primarily from blood (44.8%) and sputum (19.3%). Eleven ARGs were identified, with <em>sde</em> being the most prevalent. Carbapenemase genes included <em>bla</em>SME, <em>bla</em>KPC, and <em>bla</em>NDM-1, though co-occurrence in individual strains was absent. Novel ARGs, including <em>armA, rmtC</em>, and <em>fosA7.2</em>, were reported. Among 855 genomes with identified STs, ST366, ST367, ST365, and ST423 were most common. Phylogenetic analysis highlighted significant genetic diversity and distinct evolutionary lineages.</div></div><div><h3>Conclusion</h3><div>Temporal analysis showed a genome peak in 2019, underscoring the global prevalence and adaptability of <em>S. marcescens</em>. The distribution of ARGs across diverse STs emphasizes horizontal gene transfer as a key driver of resistance. Judicious antibiotic use is essential to mitigate further resistance.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 81-89"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surveillance and characteristics of vancomycin-resistant Enterococcus isolates in a Chinese tertiary hospital in Shenzhen, 2018 to 2024","authors":"Hongwei Shen ,&nbsp;Qiaomin Zhang ,&nbsp;Shaobo Li ,&nbsp;Tingting Huang ,&nbsp;Wen Ma ,&nbsp;Daming Wang ,&nbsp;Peng Li","doi":"10.1016/j.jgar.2024.11.002","DOIUrl":"10.1016/j.jgar.2024.11.002","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the prevalence and characteristics of vancomycin-resistant <em>Enterococcus</em> (VRE) isolates in a Chinese tertiary hospital in Shenzhen.</div></div><div><h3>Methods</h3><div>A hospital-based retrospective epidemiological survey of <em>Enterococcus</em> was conducted over a 6.5-y period, from January 2018 to June 2024. The VRE isolates were identified and subjected to screening for the six <em>van</em> genes and multi-locus sequence typing (MLST) genotyping. The clinical characteristics, antimicrobial susceptibility profiles and molecular features were subjected to analysis.</div></div><div><h3>Results</h3><div>A total of 34 non-duplicate VRE isolates were identified, comprising 32 vancomycin-resistant <em>Enterococcus faecium</em> (VREfm) and 2 vancomycin-resistant <em>Enterococcus faecalis</em> (VREfa) strains. Since its initial isolation in 2022, there has been an observable increase in the detection rate of VRE. The detection rate of VRE between 2022 and 2024 (until June) was 0.3%, 4.8%, and 8.6%, respectively. The majority of the VRE strains were isolated from urine (25/34, 73.5%), and the highest detection rate (9.1%) of VRE infections was observed in the patients aged ≥75 y. In excess of 90% of VRE isolates exhibited resistance to ciprofloxacin (97.1%) and levofloxacin (97.1%), followed by ampicillin (94.1%) and penicillin (94.1%). The non-susceptible rate was observed to be low for linezolid (2.9%) and tigecycline (5.9%). Of the 29 VREfm isolates preserved and tested, 28 were found to harbour the <em>vanA</em> gene. A total of six STs were identified among the 29 VREfm isolates, with ST80 (16/29, 55.2%) being the predominant. The ST80 remained the most prevalent clone until the introduction of ST78 in May 2023, at which point these two clones became the most prevalent.</div></div><div><h3>Conclusion</h3><div>There has been an observable increase in the prevalence of VRE in our hospital since 2022. Furthermore, an ongoing outbreak of ST80 and ST78 VREfm with <em>vanA</em>-harboring plasmid was identified. It is imperative that continuous surveillance be conducted in order to inform public health interventions.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 29-33"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and antimicrobial resistance of three clones (ST1223, ST2198, ST2250) of Staphylococcus argenteus clinical isolates in northern Japan","authors":"Meiji Soe Aung ,&nbsp;Noriko Urushibara ,&nbsp;Mitsuyo Kawaguchiya ,&nbsp;Nobuhide Ohashi ,&nbsp;Mina Hirose ,&nbsp;Kenji Kudo ,&nbsp;Masahiko Ito ,&nbsp;Nobumichi Kobayashi","doi":"10.1016/j.jgar.2024.11.004","DOIUrl":"10.1016/j.jgar.2024.11.004","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Staphylococcus argenteus</em>, a newly described species of coagulase-positive <em>Staphylococcus</em>, was first identified in clinical isolates in northern Japan in 2019. The aim of the present study was to clarify the trends in the epidemiological features and antimicrobial resistance traits of <em>S. argenteus</em> in the recent years.</div></div><div><h3>Methods</h3><div><em>S. argenteus</em> isolates from various clinical specimens were screened using MALDI-TOF and genotyped using the multilocus sequence typing scheme. Antimicrobial susceptibility was determined using broth microdilution test and resistance genes were detected by multiplex/uniplex PCR. Nucleotide sequences of haemolysin genes (<em>hla, hlb, hld</em>, and <em>hlg</em>) were determined and analysed in some isolates.</div></div><div><h3>Results</h3><div>From August 2020 to December 2023, 210 isolates were identified as <em>S. argenteus</em>. The ratio of <em>S. argenteus</em> to <em>Staphylococcus aureus</em> clinical isolates was 0.0045, showing a slightly decreasing trend compared to what was previously reported (2019.8–2020.7). Three genotypes, ST1223-<em>coa</em>-XV, ST2198-<em>coa</em>-XIV, and ST2250-<em>coa</em>-XId were identified, with ST2250 being dominant (49%) and exhibiting an increasing trend. <em>S. argenteus</em> isolates were almost susceptible to antimicrobials examined, while ST2198 isolates showed higher resistance rates to ampicillin, macrolides, and aminoglycosides than other clones, harbouring <em>blaZ, msrA</em>, and <em>aac(6′)-Ie-aph(2″)-Ia</em>. Only eight isolates (4-ST2250, 3-ST1223, and 1-ST2198) did not show haemolysis on blood agar plates. These isolates had intact haemolysin genes and promoter regions; however, a novel deletion mutation in <em>hlg</em>-component A was detected in some haemolytic and non-haemolytic isolates.</div></div><div><h3>Conclusions</h3><div>The present study revealed the epidemiological trend of <em>S. argenteus</em> clones in northern Japan, along with the occurrence of non-haemolytic isolates with intact haemolysin genes.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 1-7"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing trend of antimicrobial resistance among methicillin-resistant Staphylococcus aureus strains in Southwest Finland, 2007–2016: An analysis of shifting strain dynamics and emerging risk factors","authors":"Jaakko Silvola ,&nbsp;Kirsi Gröndahl-Yli-Hannuksela ,&nbsp;Tiina Hirvioja ,&nbsp;Kaisu Rantakokko-Jalava ,&nbsp;Mari Kanerva ,&nbsp;Kari Auranen ,&nbsp;Harri Marttila ,&nbsp;Jenna Junnila ,&nbsp;Jaana Vuopio","doi":"10.1016/j.jgar.2024.11.015","DOIUrl":"10.1016/j.jgar.2024.11.015","url":null,"abstract":"<div><h3>Objective</h3><div>Substantial rise in the annual incidence of methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) was reported in Southwest Finland (12.4–24.9/100,000 people) between 2007 and 2016. To understand the implications of these changes to the management of MRSA, we sought to analyse the antimicrobial resistance (AMR) trends of MRSA in relation with patient characteristics.</div></div><div><h3>Methods</h3><div>Antimicrobial susceptibility was determined for 10 clinically relevant antimicrobials. Strains with resistance to ≥2 antimicrobials were defined multi-resistant. The isolates were spa-typed and clustered. AMR trends and risk factors were identified by associating resistant phenotypes with patient demographics.</div></div><div><h3>Results</h3><div>A total of 983 new MRSA cases were identified between 2007 and 2016. After 2011, significant increasing trends were observed in the proportion of isolates resistant to clindamycin (13.9%–31.5%, <em>P</em> &lt; 0.001), erythromycin (19.4%–35.4%, <em>P</em> &lt; 0.001) and tetracycline (16.7%–32%, <em>P</em> &lt; 0.001). The proportion of multi-resistant isolates more than doubled from 14.8% to 39.2%. The increasing AMR trend was reflected in the increase of new strain types and the decrease of previously dominant, non-multi-resistant strains. Patient risk factors associated with (<em>P</em> <em>&lt;</em> 0.001) the acquisition of multi-resistant strains included community acquisition, livestock contact, hospital care abroad and immigrant status.</div></div><div><h3>Conclusions</h3><div>Notable increasing AMR trends among MRSA isolates were observed in Southwest Finland, 2007–2016. The shift in patient demographics to younger age groups and community acquisition contributed to the increase in multi-resistant strains. Immigration, contact with hospital environment abroad and contact with livestock were identified as essential risk factors of multi-resistance. The increased level of co-resistance has persisted after 2016.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 47-52"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas aeruginosa ST1971 clinical strain carrying the blaNDM-1 gene on ICETn43716385 in Greece","authors":"Christos-Georgios Gkountinoudis,&nbsp;Efthymia Petinaki","doi":"10.1016/j.jgar.2024.12.003","DOIUrl":"10.1016/j.jgar.2024.12.003","url":null,"abstract":"","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 98-99"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Draft genome sequence of a co-harbouring blaNDM-5 and mcr-1.1 Escherichia coli phylogroup A isolate associated with patient colonisation in Ireland","authors":"Anna Tumeo ,&nbsp;Francesca McDonagh ,&nbsp;Aneta Kovarova ,&nbsp;Kate Ryan ,&nbsp;Christina Clarke ,&nbsp;Georgios Miliotis","doi":"10.1016/j.jgar.2024.11.018","DOIUrl":"10.1016/j.jgar.2024.11.018","url":null,"abstract":"<div><h3>Objectives</h3><div>While <em>Escherichia coli</em> phylogroup-A is typically associated with commensal strains, some isolates can harbour virulence and exhibit multidrug-resistant (MDR) phenotypes. We report the draft genome of a rare instance of carbapenem, fosfomycin and colistin resistant <em>E. coli</em> phylogroup-A, isolated as part of routine screening of a human patient in a clinical setting in Ireland.</div></div><div><h3>Methods</h3><div><em>E. coli</em> E230738 was identified using MALDI-ToF/MS. Antibiotic susceptibility testing was performed using the Sensititre-EUMDRXXF plate. Whole-genome-sequencing was conducted with NextSeq1000, and genomic analysis identified antibiotic-resistance-genes (ARGs) and virulence-factors (VFs). Phylogenetic analysis was performed using whole-genome-multilocus-sequence-typing (wgMLST).</div></div><div><h3>Results</h3><div><em>E. coli</em> E230738 genome was identified to belong to phylogroup-A/ST10 complex and to harbour 63 ARGs, 17 of which acquired. Resistance to beta-lactams, including carbapenems and cephalosporins was likely due to chromosomally identified <em>bla</em>_NDM-5. Colistin resistance appeared associated with acquired <em>mcr</em>-1.1. Despite lacking fosfomycin-inactivating-enzymes, fosfomycin resistance was observed, possibly due to efflux pumps. Forty-seven chromosomal VFs were identified, involved in adhesion and iron acquisition amongst other properties. Plasmid replicons associated with the spread of MDR genes such as IncHI2/HI2A were detected. wgMLST analysis showed the closest relative being a strain from the UK, exhibiting differences in the sequences of 851 genes.</div></div><div><h3>Conclusion</h3><div>This is a first detected instance of a <em>bla</em>_NDM-5 and <em>mcr</em>-1.1 co-occurring in <em>E. coli</em> in Ireland. The MDR profile of <em>E. coli</em> E230738 highlights the growing public health concern posed by the dissemination of MDR <em>E. coli</em> lineages with limited treatment options and underscores the need for clinical screening coupled with genomic surveillance to better understand evolving MDR patterns in <em>E. coli</em>.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 62-65"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}