Journal of global antimicrobial resistance最新文献

{"title":"Whole genome sequencing of non-aureus Staphylococcus species from poultry barn bioaerosols across Alberta reveals circulation of isolates with multiple antibiotic resistant genes and disinfectant resistant genes","authors":"Awais Ghaffar ,&nbsp;Karen Liljebjelke ,&nbsp;Sylvia Checkley ,&nbsp;Frank van der Meer ,&nbsp;Muhammad Farooq ,&nbsp;Heshanthi Herath Mudiyanselage ,&nbsp;Mohamed Faizal Abdul-Careem","doi":"10.1016/j.jgar.2025.03.014","DOIUrl":"10.1016/j.jgar.2025.03.014","url":null,"abstract":"<div><h3>Objectives</h3><div>Poultry environment has vast variety of bacterial species and non-<em>aureus Staphylococcus</em> species (NASS) are predominantly found. Various NASS are normal inhabitant of gastrointestinal tract and skin of chickens. The study objective was to isolate and identify NASS from bioaerosols of layer chicken barns across Alberta, leading to the phenotypic and genotypic antimicrobial resistance (AMR) profiling of the isolated bacterial colonies.</div></div><div><h3>Methods</h3><div>A total of 15 barns were sampled in this study with an XMX-CV microbial air sampler. Phenotypic AMR profiles were determined using the Sensititre® broth microdilution method on the standard CMV3AGPF plates and whole genome sequencing (WGS) was conducted to confirm the species and AMR genes.</div></div><div><h3>Results</h3><div>Seven <em>Staphylococcus equorum</em>, 5 <em>Staphylococcus shinii</em>, 1 <em>Staphylococcus pseudoxylosus</em>, 1 <em>Staphylococcus cohnii</em>, and 1 <em>Staphylococcus gallinarum</em> isolates were confirmed and identified by WGS and comparative genomic analysis. One of the <em>S. equorum</em> isolate was genetically highly divergent from the remaining isolates of the study. The phenotypic susceptibility profile showed 73.3% (11/15) of the isolates were resistant to lincomycin, 66.7% (10/15) were resistant to tetracycline, 33.3% (5/15) were resistant to streptomycin, 20% (3/15) were resistant to erythromycin, and 6.3% were resistant to gentamicin. A total of 10 antimicrobial resistance genes (ARGs) for antibiotics and 1 gene encoding resistance to disinfectants were detected among the study isolates by WGS.</div></div><div><h3>Conclusions</h3><div>Normal bioaerosol microflora having ARGs may lead to reduced therapeutic effectiveness of antibiotics in poultry and these ARGs can be a source of serious public health concern.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 18-26"},"PeriodicalIF":3.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of an F2:A-:B- epidemic plasmid harbouring tet(X4) in Escherichia coli isolated from a pig slaughterhouse in China","authors":"Xin Huang ,&nbsp;Xing-Xing Zhang ,&nbsp;Yue Jiang ,&nbsp;Peng-Yun Shao ,&nbsp;Qin-Chun Ma ,&nbsp;Xue-Qin Li ,&nbsp;Xinan Jiao ,&nbsp;Qiuchun Li ,&nbsp;Fa-Gang Zhong ,&nbsp;Jing Wang","doi":"10.1016/j.jgar.2025.03.013","DOIUrl":"10.1016/j.jgar.2025.03.013","url":null,"abstract":"","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 15-17"},"PeriodicalIF":3.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights of predominant international high-risk clone ST2 Acinetobacter baumannii isolates in Saudi Arabia","authors":"Thamer M. Brek ,&nbsp;Abdulrahman A. Muhajir ,&nbsp;Khalil K. Alkuwaity ,&nbsp;Moayad A. Haddad ,&nbsp;Elaf M. Alattas ,&nbsp;Zaki M. Eisa ,&nbsp;Majid S. Al-Thaqafy ,&nbsp;Ahmed M. Albarraq ,&nbsp;Ibrahim A. Al-Zahrani","doi":"10.1016/j.jgar.2025.03.012","DOIUrl":"10.1016/j.jgar.2025.03.012","url":null,"abstract":"<div><h3>Objectives</h3><div>Carbapenem-resistant <em>Acinetobacter baumannii</em> (CRAB), particularly the globally prevalent ST2 clone, poses significant threats in the Gulf Cooperation Council region through extensive drug resistance, high virulence, and efficient gene transfer. This study investigates the epidemiological and genomic characteristics of CRAB in the Jazan region, using whole genome sequencing to understand its transmission and genetic determinants.</div></div><div><h3>Methods</h3><div>Sixty-five clinical <em>A. baumannii</em> isolates exhibiting phenotypic resistance to carbapenems (meropenem, imipenem, and ertapenem) were collected from various tertiary hospitals in the Jazan region. The presence of prevalent carbapenemase genes among these isolates was examined using two sets of multiplex polymerase chain reaction (PCR). Forty-eight isolates confirmed as carbapenemase producers were selected for WGS analysis.</div></div><div><h3>Results</h3><div>Among the 65 clinical CRAB isolates, the adult ICU exhibited the highest prevalence of CRAB (76.9%). Multiplex PCR identified 48 (73.8%) isolates as carbapenemase producers, all harboring <em>bla</em>_OXA-51-like genes. Specifically, <em>bla</em>_OXA-23 was detected in 39 isolates (60%), <em>bla</em>_NDM in 8 (12.3%), and <em>bla</em>_OXA-24 in one isolate. These 48 carbapenemase-producing isolates also carried virulence-associated genes related to adherence, biofilm formation, immune evasion, iron acquisition and regulation, and serum resistance. Whole-genome sequencing (WGS) revealed that 39 (81.2%) of the carbapenemase-positive isolates belonged to ST2, followed by ST85 and ST107, with the KL152 locus as the most common capsule type.</div></div><div><h3>Conclusions</h3><div>The study indicates a significant rise in the ST2 clone in the Arabian Gulf, particularly in Saudi Arabia, and underscores the need for ongoing surveillance of less common clones such as ST85 and ST107 to manage public health risks effectively.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"42 ","pages":"Pages 243-252"},"PeriodicalIF":3.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wastewater-based AMR surveillance associated with tourism on a Caribbean island (Guadeloupe)","authors":"Mélanie Pimenta ,&nbsp;Maria Alexa ,&nbsp;Degrâce Batantou Mabandza ,&nbsp;Sylvain Dulaurent ,&nbsp;Bich-Tram Huynh ,&nbsp;Margaux Gaschet ,&nbsp;Lulla Opatowski ,&nbsp;Sébastien Breurec ,&nbsp;Marie-Cécile Ploy ,&nbsp;Christophe Dagot","doi":"10.1016/j.jgar.2025.03.010","DOIUrl":"10.1016/j.jgar.2025.03.010","url":null,"abstract":"<div><h3>Objectives</h3><div>Antimicrobial resistance (AMR) is a major public health concern worldwide. International travel is a risk factor for acquiring antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs). Therefore, understanding the transmission of ARB and ARGs is instrumental in tackling AMR. This longitudinal study aimed to assess the benefit of wastewater monitoring in Guadeloupe to evaluate the role of tourism in the spread of AMR.</div></div><div><h3>Methods</h3><div>A wastewater-based surveillance (WBS) study was conducted to monitor AMR in Guadeloupe in 2022 during dry and wet seasons. We characterized the resistome, microbiome and exposome of water samples collected in wastewater treatment facilities of two cities with different levels of tourism activities, in the content of aircraft toilets, and the pumping station receiving effluents from hotels.</div></div><div><h3>Results</h3><div>The results show that the WBS approach facilitates the differentiation of various untreated effluents concerning exposome, microbiome, and resistome, offering insights into AMR dissemination. Additionally, the findings reveal that microbiome and exposome are comparable across sites and seasons, while resistome characterisation at specific locations may be pertinent for health surveillance. The microbiome of aircraft was predominantly composed of anaerobic bacteria from human intestinal microbiota, whereas the other locations exhibited a blend of human and environmental bacteria. Notably, individuals arriving by air have not introduced clinically significant resistance genes. Exposome compounds have been shown to influence the resistome's variance.</div></div><div><h3>Conclusions</h3><div>Clear differences were seen between the aircraft and the local sampling sites, indicating that the contribution of tourism to the observed resistance in Guadeloupe is not significant.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 27-34"},"PeriodicalIF":3.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Faecal microbiota transplantation to decolonize vancomycin-resistant Enterococcus: A pilot study to evaluate safety and clinical outcome","authors":"Shu-Min Lin ,&nbsp;Puo-Hsien Le ,&nbsp;Chyi-Liang Chen ,&nbsp;Yuan-Ming Yeh ,&nbsp;Hsien-Li Liao ,&nbsp;Cheng-Hsun Chiu","doi":"10.1016/j.jgar.2025.03.011","DOIUrl":"10.1016/j.jgar.2025.03.011","url":null,"abstract":"<div><h3>Objectives</h3><div>Faecal microbiota transplantation (FMT) has shown promise as a treatment for recurrent or refractory <em>Clostridioides difficile</em> infections. This study aimed to evaluate the decolonization effects of FMT on vancomycin-resistant <em>Enterococcus</em> (VRE).</div></div><div><h3>Methods</h3><div>This feasibility trial prospectively recruited patients with more than three recurrent VRE infections. FMT was performed by infusing faecal microbiota solutions from healthy, unrelated donors into the participants’ guts via colonoscopy. Faecal microbiota profiles before and after FMT were analysed.</div></div><div><h3>Results</h3><div>Three of the six patients (50%) experienced VRE decolonization after FMT, lasting over 6 months. Baseline analysis revealed that patients who achieved decolonization had greater microbial diversity compared to those with persistent VRE colonization. Throughout the study, there were no adverse events observed in the patients after FMT. Elevated alpha diversity persisted in responders, while non-responders showed no significant changes. In responders, the abundance of genera within the phylum Firmicutes (Bacillota), including <em>Anaerostipes, Blautia, Faecalibacterium</em>, and <em>Ruminococcus</em>, and the genus <em>Collinsella</em> within the phylum Actinobacteriota increased steadily through 180 days post-FMT.</div></div><div><h3>Conclusions</h3><div>FMT may leverage bacterial strain competition to facilitate decolonization of drug-resistant organisms, with successful VRE decolonization potentially linked to increased abundance of phyla Firmicutes and Actinobacteriota over 6 months.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 1-6"},"PeriodicalIF":3.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clostridium Butyricum Miyairi Bacteriocin Treatment for Clostridioides difficile Infections with Clinical Isolates: Insights from In Vitro, Ex Vivo, and Mouse Model Studies.","authors":"Ching-Chi Lee, Yi-Chen Tu, Hung-Tsung Wu, Wen-Chien Ko, Hsiao-Chieh Liu, Pei-Jane Tsai, Hsiang-Ning Chang, I-Hsiu Huang, Yuan-Pin Hung","doi":"10.1016/j.jgar.2025.03.007","DOIUrl":"https://doi.org/10.1016/j.jgar.2025.03.007","url":null,"abstract":"<p><strong>Objective: </strong>The standard antimicrobial therapy for Clostridioides difficile infections (CDIs) is limited to oral fidaxomicin or vancomycin, but these agents are associated with high treatment failure and recurrence rates. Clostridium butyricum had been proven effective in many kinds of gastrointestinal disease. With a less disturbed gut microbiota, we hypothesized that the properties of Clostridium butyricum Miyairi Bacteriocin (CBM-B) make it a potential therapeutic agent for treating patients with CDIs.</p><p><strong>Methods: </strong>The inhibitory effects of CBM-B and vancomycin were compared using the kinetic time-kill assay, ex vivo co-culture model and mouse model.</p><p><strong>Results: </strong>Among the clinical isolates of C. difficile, the minimal inhibitory concentration (MIC) of CBM-B ranged from 0.0625 to 8 µg/ml; the MIC₅₀ and MIC₉₀ were 1 µg/ml and 4 µg/ml, respectively. In the mouse model infected with a RT078 and receiving CBM-B intra-rectal enema therapy, mice infected with isolates with a relative low CBM-B MICs (2 µg/ml, abbreviated as M2) revealed significant better therapeutic effect, including less loss of body weight and cecum weight, compared with those infected with isolates of relative high CBM-B MICs (4 or 8 µg/ml, abbreviated as M4 or M8) . The relative C. difficile bacterial burden in stool of mice receiving CBM-B treatment were significantly lower among mice infected with M2, compared with that infected with M4 or M8. CBMB treatment, compared with vancomycin therapy revealed less disturbance in gut microbiota.</p><p><strong>Conclusion: </strong>CBM-B could be effective in the treatment of CDIs that infected with a C. difficile isolate with relatively low MICs with less disturbance in gut microbiota.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of a multidrug-resistant hypovirulent ST1859-KL35 klebsiella quasipneumoniae subsp. similipneumoniae strain co-harboring tmexCD2-toprJ2 and blaKPC-2","authors":"Jiawei Ding ,&nbsp;Mengying Zhang ,&nbsp;Jiyong Chang ,&nbsp;Zidan Hu ,&nbsp;Pei He ,&nbsp;Jia Wang ,&nbsp;Lei Feng","doi":"10.1016/j.jgar.2025.03.009","DOIUrl":"10.1016/j.jgar.2025.03.009","url":null,"abstract":"<div><h3>Objectives</h3><div>The rise of multidrug-resistant (MDR) <em>Klebsiella pneumoniae</em> is a significant public health threat. <em>Klebsiella quasipneumoniae</em> is often misidentified as <em>K. pneumoniae,</em> and its genetic and virulence traits remain underexplored. This study characterizes the genomic and phenotypic features of a <em>K. quasipneumoniae</em> subsp. <em>similipneumoniae</em> strain (KP24).</div></div><div><h3>Methods</h3><div>Antibiotic susceptibility was tested using microbroth dilution assay. Virulence was evaluated through serum killing assay and <em>Galleria mellonella</em> infection model. Whole genome sequencing (WGS) and bioinformatics analysis determined sequence typing, resistance profiles, and plasmid types. Conjugation assays assessed plasmid transferability, while phylogenetic analysis explored genetic relationships.</div></div><div><h3>Results</h3><div>KP24 exhibited an MDR phenotype, including resistance to carbapenems, ceftazidime/avibactam, and tigecycline. KP24 showed significantly higher serum survival and <em>G. mellonella</em> lethality than ATCC700603, though it was less virulent than the hypervirulent strain NUTH-K2044. WGS identified KP24 as ST1859 and KL35, harboring the aerobactin virulence gene cluster (<em>iucABCDiutA)</em> and multiple resistance genes, including <em>tmexCD2-toprJ2,</em> bla_KPC-2<em>_, bla_OXA-10,</em> bla_IMP-4<em>_,</em> and <em>qnrS1</em>. Notably, the <em>tmexCD2-toprJ2</em> and bla_KPC-2 genes were located on the same plasmid (pKP24–1), an uncommon co-existence. Conjugation assays confirmed the independent transferability of pKP24–1 to <em>Escherichia coli</em> J53. Phylogenetic analysis revealed that ST1859 forms a distinct monoclade with low genetic diversity, closely related to ST334, suggesting regional expansion and potential global dissemination.</div></div><div><h3>Conclusions</h3><div>KP24 represents a hypovirulent yet multidrug-resistant strain of <em>K. quasipneumoniae</em> subsp. <em>similipneumoniae,</em> with a concerning combination of virulence and resistance determinants. The co-location of <em>tmexCD2-toprJ2</em> and bla_KPC-2 on a transferable plasmid highlights the potential for horizontal gene transfer of critical resistance mechanisms.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"42 ","pages":"Pages 253-261"},"PeriodicalIF":3.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of blaNDM-5-bearing IncHI2 plasmid from Escherichia fergusonii in China","authors":"Yan Shi ,&nbsp;Yue Yang ,&nbsp;Yu Song ,&nbsp;Yujie Zhu ,&nbsp;Guoping Zhao ,&nbsp;Biao Tang","doi":"10.1016/j.jgar.2025.03.008","DOIUrl":"10.1016/j.jgar.2025.03.008","url":null,"abstract":"<div><h3>Objective</h3><div>Carbapenems are considered to be the last resort for serious infections caused by multidrug-resistant Gram-negative bacteria, and the emergence of carbapenem-resistant <em>Enterobacteriaceae</em> has posed a serious threat to human health. However, carbapenem resistance is rarely reported in <em>Escherichia fergusonii</em>. In this study, a New Delhi metallo-β-lactamase (NDM)-5-producing <em>E. fergusonii</em> strain, EFSXRJ10, was isolated from a chicken in China.</div></div><div><h3>Methods</h3><div>Minimal inhibitory concentrations were determined using broth microdilution-based antimicrobial susceptibility testing. The complete genome sequence of the NDM-positive isolate was obtained using the Illumina NovaSeq and Oxford Nanopore GridION sequencing platforms, followed by hybrid assembly with Unicycler. In the plasmid conjugation assay, a sodium azide–resistant <em>Escherichia coli</em> strain, J53, was employed as the recipient.</div></div><div><h3>Results</h3><div>Strain EFSXRJ10 was resistant to ampicillin, amoxycillin-clavulanic acid, gentamicin, spectinomycin, tetracycline, florfenicol, sulfafurazole, cefotaxime, ceftazidime, apramycin and meropenem. The <em>bla</em>_NDM-5 gene was located on the IncHI2 plasmid, which can be transferred by conjugation at a frequency of (4.78 ± 0.67) × 10^–5. The <em>bla</em>_NDM-5-carrying plasmid, which harbours 14 antibiotic resistance genes belonging to the IncHI2/ST3 type and exhibiting high similarity to other <em>bla</em>_NDM-5-carrying IncHI2 plasmids, was deposited in GenBank. The genetic structure containing <em>bla</em>_NDM-5 was organised as ‘IS<em>3000</em>-ΔIS<em>Aba125-</em>IS<em>5</em>-ΔIS<em>Aba125</em>-<em>bla</em>_NDM-5-<em>ble</em>_MBL-<em>trpF</em>-<em>dsbD</em>-IS<em>26</em>-∆<em>umuD</em>-∆IS<em>Kox3</em>-∆IS<em>3000</em>’.</div></div><div><h3>Conclusions</h3><div>This is the first report characterising the <em>bla</em>_NDM-5-bearing IncHI2 plasmid in <em>E. fergusonii</em>. Surveillance and control measures should be implemented to halt transmission of <em>bla</em>_NDM-5 in food animals.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"42 ","pages":"Pages 222-228"},"PeriodicalIF":3.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome sequencing characterisation of a new KPC-245 variant-carrying Klebsiella pneumoniae strain isolated from a transplanted patient and resistant to ceftazidime/avibactam, meropenem/vaborbactam and imipenem/relebactam","authors":"Claudia Vaiana ,&nbsp;Roberta Vazzana ,&nbsp;Salvatore Castelbuono ,&nbsp;Andrea Cona ,&nbsp;Alessandra Mularoni ,&nbsp;Rita Minucci ,&nbsp;Francesco Monaco ,&nbsp;Daniele Di Carlo ,&nbsp;Pier Giulio Conaldi ,&nbsp;Alessia Gallo ,&nbsp;Nicola Cuscino","doi":"10.1016/j.jgar.2025.03.006","DOIUrl":"10.1016/j.jgar.2025.03.006","url":null,"abstract":"<div><h3>Objective</h3><div>In recent decades, the increasing prevalence of multidrug-resistant <em>Klebsiella pneumoniae</em> carbapenemase (KPC)-carrying <em>K. pneumoniae</em> (KPC-Kp) has become a worldwide public concern. Herein, we characterised a ceftazidime/avibactam (CAZ/AVI), meropenem/vaborbactam (MER/VAB) and imipenem/relebactam (IMI/REL)-resistant KPC-Kp strain isolated from a critically ill transplant patient.</div></div><div><h3>Methods</h3><div>Antimicrobial susceptibility testing and whole-genome sequencing (WGS) were conducted to characterise the strain at phenotypic and genotypic levels. Genomic DNA was sequenced using the Illumina platform. Bioinformatic analyses were used to investigate the genome sequences both for resistance and virulence features, and for the characterisation of plasmids.</div></div><div><h3>Results</h3><div>Phenotypic characterisation revealed that the KPC-Kp isolate was highly resistant to a wide range of antibiotics, including all β-lactam/β-lactamase inhibitor combinations such as CAZ/AVI, MER/VAB, IMI/REL and cefiderocol. WGS analysis showed that the isolate, belonging to the rare lineage ST661, contained several resistance and virulence genes. Among the resistance genes, we identified a new KPC variant within the mobile genetic element Tn4401—KPC-245—characterised by the insertion of nine amino acids (RAPNKDDYT) at position 263 as well as an amino acid change within the protein sequence, E274D, compared with KPC-3. Interestingly, the presence of mutations only in the <em>bla</em>_KPC gene and not in other β-lactamase coding genes strongly points to the role of KPC-245 in β-lactam/β-lactamase inhibitor combinations and cefiderocol resistance.</div></div><div><h3>Conclusions</h3><div>In our study, by using WGS analysis on a clinical isolate, we identified a new <em>bla</em>_KPC variant within the Tn4401 transposon. Our results confirm the importance of continuous surveillance of multidrug-resistant <em>K. pneumoniae</em> in the clinical context.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"42 ","pages":"Pages 229-233"},"PeriodicalIF":3.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Draft genome of the multidrug-resistant Citrobacter freundii strain CAPA023 isolated from Arapaima gigas in Peru: Is it a reservoir of resistance and virulence genes?","authors":"Enrique Garcia-Candela ,&nbsp;Aaron Mondragón-Martínez ,&nbsp;Manuel Noceda-Rodríguez ,&nbsp;Fernando Mesias Valle ,&nbsp;Milagros Cabrera-Soregui ,&nbsp;Veronica Valverde-Vera ,&nbsp;Maria Benito-García ,&nbsp;Romina Romayna-Ríos ,&nbsp;Miriam Verástegui-Tello ,&nbsp;Saurabh Dubey ,&nbsp;Hetron M. Munang'andu ,&nbsp;Jefferson Yunis-Aguinaga","doi":"10.1016/j.jgar.2025.03.005","DOIUrl":"10.1016/j.jgar.2025.03.005","url":null,"abstract":"<div><h3>Objectives</h3><div>The purpose of this study was to analyze the genome of the multidrug-resistant <em>Citrobacter freundii</em> strain CAPA023, which was obtained from diseased <em>Arapaima gigas</em> fry. The study focused on determining mobile genetic elements and genetic factors that contribute to antibiotic resistance and pathogenicity.</div></div><div><h3>Methods</h3><div>Genomic DNA was sequenced using Illumina NovaSeq (2 × 150 bp) and assembled de novo using Shovill v1.1.0. Resistance genes, virulence factors, plasmids, and mobile elements were identified using ResFinder, CARD, PlasmidFinder, MobileFinder, PathogenFinder, and VFDB.</div></div><div><h3>Results</h3><div>The 5,059,550 bp draft genome (60 contigs, 51.5% GC) revealed resistance genes for various antibiotic classes, efflux pumps, <em>IncFIB(</em>K<em>)</em> and <em>Col440I</em> plasmids, insertion sequences, and multiple virulence genes.</div></div><div><h3>Conclusion</h3><div>Considering that this bacterium was found in diseased fish, it is possible that <em>C. freundii</em> plays an important role in the spread of virulence factors and antibiotic resistance in aquaculture environments. This highlights the importance of genomic surveillance in Amazonian aquaculture.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"42 ","pages":"Pages 204-206"},"PeriodicalIF":3.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}