Journal of global antimicrobial resistance最新文献

{"title":"Characterization of a New Transposon, Tn7772, on a Novel Plasmid From Multidrug-Resistant Escherichia coli in China.","authors":"Yong Pan, Ting Li, Yanan Zhang, Qiufan Xu, Li Yang, Yuanfeng Zhao, Jinge Xu","doi":"10.1016/j.jgar.2025.07.010","DOIUrl":"https://doi.org/10.1016/j.jgar.2025.07.010","url":null,"abstract":"<p><strong>Background: </strong>The emergence of multidrug-resistant (MDR) Escherichia coli causes a serious threat to human and animal health. To characterize the genetic environments of a novel plasmid pQL57EC-1 in an MDR E. coli strain QL57EC from feces of a healthy swine in China METHODS: The plasmid pQL57EC-1 was characterized by antimicrobial susceptibility testing, whole genome sequencing and bioinformatic analysis. The transferability of pQL57EC-1 was verified by conjugation experiments and transformation experiments RESULTS: E. coli QL57EC carries two plasmids. pQL57EC-1 belongs to the incompatibility groups IncF-FIB/IncX1, while plasmid pQL57EC-2 belongs to the incompatibility group IncF-FIB. Notably, pQL57EC-1 was a novel plasmid, containing a novel Tn7772 transposon flanked by two copies of IS26 at both ends, which can also be found on plasmids from Salmonella enterica isolated from human feces. Conjugation assays for E. coli QL57EC were unsuccessful. However, pQL57EC-1 could be transferred into E. coli DH5α by transformation experiments CONCLUSIONS: This study reported a novel plasmid pQL57EC-1 harboring a novel Tn7772 transposon from swine derived E. coli QL57EC.This finding suggests that Tn7772 may pose a threat to both human and animal health by acting as a reservoir for antibiotic resistance genes (ARGs) and facilitating cross-species transmission.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging epidemic of the predominant clusters of FC428-like Neisseria gonorrhoeae in Guangdong, China.","authors":"Yang Chen, Xiao-Lin Qin, Han Zhou, Xing-Zhong Wu, Wen-Tao Chen, Zi-Yan Zhang, Qing-Xian Zhan, Zhan-Qin Feng, Yao-Hua Xue, Yong-Fei Hu, Chi-Xing Guo, Feng Wang, Ming Li, Zhi-Zhou Wu, Jian-Hong Xie, Lian-Hui Liang, Hui-Xuan Xiao, Zheng-Qi Shi, Xue-Mei Hu, Qian Li, He-Yong Chen, Yong-Jian Ke, Wen-Ying Luo, Guan-Jun Huang, Jin-Bo Huang, Ying Peng, He-Ping Zheng","doi":"10.1016/j.jgar.2025.07.005","DOIUrl":"https://doi.org/10.1016/j.jgar.2025.07.005","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to investigate the molecular characteristics of predominant epidemic clusters of FC428-like N. gonorrhoeae in Guangdong, China in 2022.</p><p><strong>Methods: </strong>Minimum inhibitory concentrations (MICs) were determined for N. gonorrhoeae isolates collected from the Guangdong Gonococcal Antimicrobial Surveillance Program. N. gonorrhoeae multiantigen sequence typing (NG-MAST), antimicrobial resistance sequence typing (NG-STAR), multilocus sequence typing (MLST) sequence types (STs), and penA alleles were determined by whole-genome sequencing. The isolates were further characterized by phylogenetic analysis.</p><p><strong>Results: </strong>A total of 537 N. gonorrhoeae isolates were analyzed, molecular analysis revealed that 7.8% of the isolates carried penA 60.001 allele, which was highly resistant to ceftriaxone (88.1%) and cefixime (100.0%). The predominant STs in penA 60.001 isolates were MLST ST1903 (23,54.8%), ST7365 (11,26.2%), NG-STAR ST1143 (13,31.0%), ST233 (4,9.5%), ST1133 (4,9.5%), and NG-MAST ST22261 (10,23.8%). Among 537 isolates, of which 11.2% were resistant to ceftriaxone and 19.6% to cefixime. In western Guangdong, resistance to ceftriaxone and cefixime reached 17.3% and 26.9%, respectively. The most predominant types among ceftriaxone-resistant isolates are genetically closer to FC428 isolates, and differ from those among cefixime-resistant isolates. Phylogenetic analysis revealed that the Guangdong FC428-like isolates from 2021 to 2022 spread across the whole phylogenetic tree, but the majority were clustered within a distinct evolutionary clade.</p><p><strong>Conclusions: </strong>FC428-like isolates in Guangdong formed a unique evolutionary clade with high cephalosporin resistance. These findings highlight the need to revise national gonorrhea treatment guidelines and prioritize the development of new antimicrobials.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging Resistance to Novel β-Lactam β-Lactamase Inhibitor Combinations in Klebsiella pneumoniae bearing KPC Variants.","authors":"Mase Hamza, German M Traglia, Lucia Maccari, Sonia Gomez, Maria Belen Sanz, Usman Akhtar, Vyanka Mezcord, Jenny Escalante, Alejandra Corso, Cecilia Rodriguez, Christopher R Bethel, Gauri G Rao, Marcelo E Tolmasky, David Paterson, Robert A Bonomo, Fernando Pasteran, Maria Soledad Ramirez","doi":"10.1016/j.jgar.2025.07.011","DOIUrl":"10.1016/j.jgar.2025.07.011","url":null,"abstract":"<p><strong>Background: </strong>Klebsiella pneumoniae carbapenemase (KPC) variants, predominantly KPC-2 and KPC-3, are significant global resistance mechanisms, conferring resistance to many β-lactams, including carbapenems, while remaining susceptible to ceftazidime-avibactam (CZA). Recently, new KPC variants have developed resistance to CZA through mutations, insertions, or deletions in regions such as the Ω-loop, 240-loop (237-243 aa), and 270-loop (266-275 aa). This study investigated collateral resistance to cefiderocol (FDC) and cefepime/zidebactam (FPZ) in isolates with these mutations.</p><p><strong>Methods: </strong>Fifteen clinical KPC-producing Klebsiella spp. isolates representing 15 distinct variants were analyzed. Antimicrobial susceptibility testing determined the MICs for CZA, carbapenems, FDC, FPZ, and other antibiotics. Synergy between CZA and FDC was assessed. Whole-genome sequencing (WGS) was used to identify resistance-related mutations.</p><p><strong>Results: </strong>CZA resistance was confirmed in 12/15 variants. Collateral resistance to FDC occurred in eight isolates, with five exhibiting spontaneous resistant subpopulations. Six FDC-resistant strains had mutations in the 270-loop (266-275 aa). FPZ resistance was seen in three KPC variants, especially those with mutations in the 270-loop, though many Ω-loop and 240-loop (237-243 aa) mutants remained susceptible. WGS of FDC-resistant subpopulations revealed additional mutations in ompC, rpoC, dksA, and cirA.</p><p><strong>Conclusions: </strong>Emerging CZA-resistant KPC variants often exhibit collateral FDC resistance, with FPZ seen less frequently. Mutations in bla_KPC, cirA, and other genes contribute to resistance. Understanding these emerging resistant patterns linked with new KPC variants is crucial to inform therapeutic decisions, as emerging resistance may limit last-line treatment options in clinical settings.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship Between Pneumococcal Serotypes and Antimicrobial Resistance: A Systematic Review and Meta-analysis.","authors":"Onyansaniba K Ntim, Aaron Awere-Duodu, Samuel Addo Akwetey, Fleischer C N Kotey, Eric S Donkor","doi":"10.1016/j.jgar.2025.07.008","DOIUrl":"https://doi.org/10.1016/j.jgar.2025.07.008","url":null,"abstract":"<p><strong>Background: </strong>The multiple serotypes of pneumococcus make vaccination and treatment challenging. Treatment complications are a result of different serotypes exhibiting distinct resistance rates. This study aims to comprehensively estimate the antimicrobial resistance rates of pneumococcal serotypes.</p><p><strong>Method: </strong>A comprehensive search was conducted in PubMed, Scopus, Web of Science, and ScienceDirect. A random effect meta-analysis was used to pool the number of isolates resistant to a particular antimicrobial per total number of isolates tested for each pneumococcal serotype RESULTS: Ninety studies were included in the analysis. The study identified 66 pneumococcal serotypes, with vaccine serotypes such as 19A, 19F, 23F, 6B, 14, 6A, 3, and 9V being the most frequently reported. Non-vaccine serotypes like 15A, 6C, 23A, 23B, 15B, and 35B were also prevalent. Most serotypes were associated with pneumococcal diseases. Serotypes exhibited varying and high resistance to cefuroxime, co-trimoxazole, tetracycline, macrolides, and clindamycin. The highest multidrug resistance was observed in serotypes 23F (63.34%, 95% CI [25.77; 94.31]), 15C (61.04%, 95% CI [0.74; 100.00]), 23A (57.28%, 95% CI [27.60; 84.58]), 19F (55.95%, 95% CI [30.26; 80.22]), 19A (54.07%, 95% CI [32.60; 74.90]), 15B (47.10%, 95% CI [9.66; 86.41]), 24F (45.77%, 95% CI [3.79; 91.31]), 15A (44.28%, 95% CI [29.58; 59.26]), and 6B (43.79%, 95% CI [28.48; 59.12]).</p><p><strong>Conclusion: </strong>This study highlights significant antimicrobial resistance among both vaccine (19A, 19F, 23F, 14, 6A, and 6B) and non-vaccine types (15A, 6C, 23A, 20, 15C, and 35B). Our findings emphasize the need for effective surveillance and targeted interventions to fight these resistant pneumococcal serotypes.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salmonella Minnesota sequence type 548 harboring a type 2 IncC megaplasmid of antimicrobial resistance and virulence (pESM) infecting a companion animal.","authors":"Luciana Sartori, João Pedro Rueda Furlan, Fábio P Sellera, Fernanda B Barbosa, Bruna Fuga, Gregory Batista Melocco, Karine Sousa Dantas, Thais Martins-Gonçalves, Ronan Adler Tavella, Ana Cristina Gales, Nilton Lincopan, Terezinha Knöbl","doi":"10.1016/j.jgar.2025.07.007","DOIUrl":"https://doi.org/10.1016/j.jgar.2025.07.007","url":null,"abstract":"<p><strong>Objectives: </strong>This study reports the identification and genomic characteristics of Salmonella strains isolated from a blood sample of a 9-year-old male Persian cat with a systemic infection (strain M885) and from a pleural effusion sample of a 9-year-old male Bulldog (strain T886) in Brazil.</p><p><strong>Methods: </strong>Genomic DNA was sequenced using the Illumina NextSeq platform, de novo assembled by SPAdes v.3.15.2, and annotated by RAST server. Serovars, sequence types (ST), antimicrobial resistance genes, plasmid replicons, Salmonella pathogenicity islands, and single nucleotide polymorphisms (SNP) analysis were accomplished by bioinformatic tools.</p><p><strong>Results: </strong>Strains M885 and T886 belonged to the serovars Sandiego (ST126) and Minnesota (ST548), respectively. Strain T886 carried bla_CMY-2 and qnrB19 onto IncC2 and Col(pHAD28) plasmids, respectively. The bla_CMY-2-bearing IncC2 plasmid also harbored mercury tolerance genes and the yersiniabactin virulence gene cluster, being classified as a type 2 IncC megaplasmid of antimicrobial resistance and virulence (pESM, plasmid for emergent S. Minnesota). SNP-based analysis revealed clonal relatedness between T886 strain with a CMY-2-producing S. Minnesota ST548 previously isolated from chicken sausage in Brazil, supporting a common ancestral origin.</p><p><strong>Conclusions: </strong>This study underscores the importance of monitoring S. enterica as the causative agent of extra-intestinal infections in small animal medicine. Therefore, the transmission dynamics and effective strategies for managing infections produced by multidrug-resistant clones adapted to the human-animal-environmental interface warrant further investigation.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful use of cefepime/zidebactam in deep-seated infections due to extensively drug-resistant Pseudomonas aeruginosa - a case series.","authors":"Pooja Shah, Ayyanraj Neeravi, Rajiv Karthik, Christy Merin Mathew, Julie Hephzibah, Hanna Elizabeth Johnson, Balaji Veeraraghavan, Abi Manesh","doi":"10.1016/j.jgar.2025.07.009","DOIUrl":"https://doi.org/10.1016/j.jgar.2025.07.009","url":null,"abstract":"<p><strong>Background: </strong>There is a paucity of safe and effective treatment options for extensively drug resistant (XDR) Gram-negative pathogens that often harbor varied resistance mechanisms concurrently. Cefepime/zidebactam (FEP/ZID) is a novel β-lactam/β-lactam enhancer (BL-BLE) combination, currently in Phase 3 trials, that has been shown to be effective against such difficult to treat resistant isolates in in-vitro studies and scattered case reports. We present a series of deep seated infections due to XDR Pseudomonas Aeruginosa (PsA), successfully treated with FEP/ZID under compassionate use.</p><p><strong>Method: </strong>Between September 2023 and May 2024, using clinico-radiographic and microbiologic (phenotypic drug susceptibility and whole genome sequencing) data, we identified five XDR PsA related infections that could not be safely treated with currently available antibiotics. All patients received FEP/ZID after obtaining informed consent and necessary approvals.</p><p><strong>Results: </strong>Four cases involved osteoarticular disease while the fifth was an endovascular graft infection. Clinical specimens yielded XDR PsA isolates that harbored varied (enzymatic and non-enzymatic) resistance mechanisms to anti-pseudomonal cephalosporins, carbapenems, aminoglycosides, fluoroquinolones and newer β-lactam/β-lactamase inhibitors. All isolates were susceptible to FEP/ZID and were treated with the same for 2-6 weeks. Clinico-radiographic assessments confirmed significant resolution of lesions in all cases. There were with no drug-related adverse events.</p><p><strong>Conclusion: </strong>The in-vitro susceptibility of our XDR PsA isolates to FEP/ZID translated to a successful clinical outcome in our series of deep-seated infections. FEP/ZID is a potential safe and effective treatment option for such serious Gram-negative infections.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic analysis of azole-resistant Aspergillus fumigatus isolated from domestic and imported tulip bulbs in Japan.","authors":"Satomi Uehara, Hiroki Takahashi, Yukari Nishino, Yumi Takahashi, Takashi Chiba, Keiko Yokoyama, Hirofumi Miyake, Kenji Sadamasu, Daisuke Hagiwara","doi":"10.1016/j.jgar.2025.07.006","DOIUrl":"https://doi.org/10.1016/j.jgar.2025.07.006","url":null,"abstract":"<p><strong>Objectives: </strong>Azole-resistant Aspergillus fumigatus (ARAF) poses a significant clinical threat and is increasingly being isolated from agricultural environments. ARAF was recently reported to be able to attach to plant bulbs and to cross national borders through international trade. In this study, we investigated the ARAF isolation rate of imported and domestic tulip bulbs that were sold in Japan.</p><p><strong>Methods: </strong>A. fumigatus was sought from tulip bulbs purchased in Japan. The cyp51A gene was sequenced to determine possession of tandem repeats (TRs) and single nucleotide variants for the isolates. Whole genome analysis was conducted to compare clinical strains, and drug susceptibility testing for itraconazole, voriconazole, and posaconazole were performed.</p><p><strong>Results: </strong>A total of 129 A. fumigatus strains were isolated from 204 imported Netherlands bulbs purchased in Japan in 2020-2022, whereas 58 strains were isolated from 209 domestic Japanese bulbs. A TR in the cyp51A promoter (azole resistance-related mutation) was detected in 24.0% of the isolates (31/129) from Netherlands bulbs and 51.7% of the isolates (30/58) from Japanese bulbs. Phylogenetic clustering using genomic data of isolates from imported Netherlands and Japanese tulip bulbs showed that TR-harboring and non-TR isolates were clearly separated into different clusters. Japanese bulb isolates with TR were genetically close to environmental and clinical isolates from the Netherlands as well as isolates from bulbs imported from the Netherlands.</p><p><strong>Conclusions: </strong>The high frequency of ARAF isolation from the bulb bred in Japan was observed through multiple-year survey.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico design of sgRNAs with knockout potential for the adeB gene of the AdeABC efflux pump of Acinetobacter baumannii.","authors":"José Miguel Benigno Delgado Ruitón, Pedro Jorge Chimoy Effio, Guillermo Uceda Campos, Edgar Enrique Llontop Cornejo, Erick Giancarlo Suclupe Farro","doi":"10.1016/j.jgar.2025.07.001","DOIUrl":"https://doi.org/10.1016/j.jgar.2025.07.001","url":null,"abstract":"<p><strong>Background: </strong>The efflux pump AdeABC, regulated by the AdeRS operon, is a key factor in multidrug resistance in Acinetobacter baumannii. The adeB gene encodes the transmembrane transporter protein, making it a promising target for therapeutic gene disruption.</p><p><strong>Methods: </strong>We designed and analyzed single guide RNAs (sgRNAs) targeting the adeB gene using CHOPCHOP. Conservation analysis was conducted with BLAST and multiple alignment tools. Potential off-target effects were assessed using Cas-OFFinder. The thermodynamic stability and accessibility of top-ranked sgRNAs were evaluated using Mfold. Structural and functional domain mapping was performed based on the 3D model of AdeB protein. Phylogenetic analyses of adeB alleles were carried out using MAFFT and iTOL.</p><p><strong>Results: </strong>Among 49 designed sgRNAs, five candidates met stringent criteria for cleavage efficiency, GC content, conservation across strains, and low off-target potential. Secondary structure analysis confirmed their stability, and domain mapping showed that the sgRNAs targeted critical regions of AdeB, including transmembrane helices and substrate-binding sites. Phylogenetic clustering confirmed high sequence conservation in clinical strains.</p><p><strong>Conclusion: </strong>This study presents three validated sgRNAs with knockout potential against the adeB gene of A. baumannii. These sgRNAs may serve as effective molecular tools to disrupt the AdeABC pump and combat efflux-mediated antimicrobial resistance. Further in vitro validation is proposed to assess their functional impact.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing access to antimicrobial resistance diagnostics for the marginalized: Challenges and opportunities of point-of-care technologies.","authors":"Vinay Modgil, Sundeep Sahay, Neelam Taneja, Arunima Mukherjee, Neha Joshi, Arun Mitra, Biju Soman, Ravikant Singh, Oshin Sinha, Rashmi Surial","doi":"10.1016/j.jgar.2025.06.023","DOIUrl":"https://doi.org/10.1016/j.jgar.2025.06.023","url":null,"abstract":"<p><p>Inadequate access to antimicrobial resistance (AMR) diagnostics remains a critical challenge, particularly in low- and middle-income countries (LMICs). Despite various global policy commitments, translating these policies into practice is restricted by socio-economic barriers, technological limitations, and infrastructure gaps. Based on ongoing empirical work in India under EquityAMR, diagnostic inequities were examined in two contrasting states. The spatial analysis demonstrated disproportionate diagnostic services, due to urban-rural and hilly-plains regions. Further laboratory assessments across 14 health institutions in these two states highlighted critical gaps in testing capacity, quality control, equipment, and data management. In addition, the potential of existing point-of-care technologies in expanding access were examined based on WHO criteria of clinical preparedness, scalability, and sustainability, although none were considered suitable for all three criteria. The study concluded that by arguing in the current scenario, traditional culture-based microbiology facilities can be established in sub-district facilities with some success in expanding access.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144618582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the tet(M)-bearing transposon Tn7125 of Escherichia coli strain A13 isolated from an intensive pig farm located in Henan province, China.","authors":"Yingying Liu, Hanmeng Ren, Jiaqi Zeng, Xinyue Zhang, Mingcheng Wang, Zhu Qiao, Yan Ma, Chaoying Liu, Huili Xia, Gongzheng Hu, Enzhong Li","doi":"10.1016/j.jgar.2025.06.022","DOIUrl":"https://doi.org/10.1016/j.jgar.2025.06.022","url":null,"abstract":"<p><strong>Background: </strong>Transposons carrying tet(M) in Gram-positive bacteria have been reported extensively, while there is a paucity of data on the transmission characteristics of tet(M) in Gram-negative bacteria. Therefore, the aim of this study was to investigate the genetic characteristics of the tet(M)-bearing transposon Tn7125, and to clarify the transmission mechanism of the plasmids pTA13-1 and pTA13-3 in Escherichia coli strain A13.</p><p><strong>Methods: </strong>Plasmids from strain A13 and a corresponding transconjugant were determined by whole genome sequencing and analyzed using bioinformatics tools. The plasmids pTA13-1 and pTA13-3 of the transconjugant TA13 were characterized by S1-pulse-field gel electrophoresis, Southern hybridization, stability experiments, and direct competition assays.</p><p><strong>Results: </strong>The conjugated IncF2:A6:B20 plasmid pTA13-1 co-transferred with the 41-kb plasmid pTA13-3, which carried no resistance genes; plasmid pTA13-2, which harbored the replication initiator PO111; and the IncX4 plasmid pTA13-4, which harbored the antibiotic resistance gene mcr-1. The novel IS26-bracked composite transposon Tn7125 was located on plasmid pTA13-1, which mainly consists of three resistance modules: IS26-ctp-lp-tet(M)-hp-IS406tnp, qac-aadA1-cmlA1-aadA2-DUF1010-dfrA12, and ∆ISVSa3-VirD-floR-LysR-ISVSa3. The plasmid pTA13-1 was highly stable in E. coli strain J53 with no fitness cost to the host or disadvantage in growth competition.</p><p><strong>Conclusion: </strong>Evolution of co-integrated transposons, such as Tn7125, may convey antibiotic resistance to a wide spectrum of hosts via the plasmids pTA13-1 and pTA13-3, which acts as an adaptable and mobile multidrug resistance reservoir to accelerate dissemination of other genes by co-selection, thereby posing a potentially serious barrier to clinical treatment regimens.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}