Journal of global antimicrobial resistance最新文献

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Refining the gut colonization Zophobas morio larvae model using an oral administration of multidrug-resistant Escherichia coli. 利用口服耐多药大肠杆菌改进 Zophobas morio 幼虫肠道定植模型。
IF 3.7 3区 医学
Journal of global antimicrobial resistance Pub Date : 2024-11-02 DOI: 10.1016/j.jgar.2024.10.262
Yasmine Eddoubaji, Claudia Aldeia, Dierik H Heg, Edgar I Campos-Madueno, Andrea Endimiani
{"title":"Refining the gut colonization Zophobas morio larvae model using an oral administration of multidrug-resistant Escherichia coli.","authors":"Yasmine Eddoubaji, Claudia Aldeia, Dierik H Heg, Edgar I Campos-Madueno, Andrea Endimiani","doi":"10.1016/j.jgar.2024.10.262","DOIUrl":"https://doi.org/10.1016/j.jgar.2024.10.262","url":null,"abstract":"<p><strong>Background: </strong>The darkling beetle Zophobas morio can be implemented as an alternative in vivo model to study different intestinal colonization aspects. Recently, we showed that its larvae can be colonized by multidrug-resistant Escherichia coli strains administered via contaminated food (for 7 days) for a total experimental duration of 28 days.</p><p><strong>Method: </strong>In the present work, we aimed to shorten the model to 14 days (T14) by administering the previously used CTX-M-15 ESBL-producing ST131 Escherichia coli strain Ec-4901.28 via a single oral administration (5 µL dose of 10<sup>8</sup> CFU/mL) , using a blunt 26s-gauge needle connected to a 250 μL gastight syringe. Force-feeding was performed either without or with (larvae placed on ice for 10 minutes before injection) anesthesia. In addition, phage-treated larvae were orally injected with 10 µL of INTESTI bacteriophage cocktail (∼10<sup>5-6</sup> PFU/mL) on days 4 (T4) and 7 (T7) .</p><p><strong>Results: </strong>Growth curve analyses showed that, while larvae rapidly became colonized with Ec-4901.28 (T1, ∼10<sup>6-7</sup> CFU/mL) , only those anesthetized maintained a high bacterial load (∼10<sup>2-3</sup>vs. ∼10<sup>5-6</sup> CFU/mL) and survival rate (76% vs. 99%; P<0.001) by T14. Moreover, bacteriophage administration to anesthetized larvae significantly reduced the bacterial count of INTESTI-susceptible Ec-4901.28 at T14 (5.17 × 10<sup>5</sup>vs. 2.26 × 10<sup>4,</sup> for non-treated and phage-treated larvae, respectively; P=0.04) .</p><p><strong>Conclusion: </strong>The methodological refinements applied to establish the intestinal colonization model simplify the use of Z. morio larvae, facilitate prompt evaluation of novel decolonization approaches and reduce experiments involving vertebrate animals in accordance with the Replacement, Reduction and Refinement principles.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study of the mechanisms of heteroresistance to colistin in a strain of Enterobacter cloacae by random mutagenesis. 通过随机诱变研究一株泄殖肠杆菌对可乐定的异抗性机制。
IF 3.7 3区 医学
Journal of global antimicrobial resistance Pub Date : 2024-10-30 DOI: 10.1016/j.jgar.2024.10.260
Romane Milleville, Linda Hadjadj, Jean-Marc Rolain, Sophie Alexandra Baron
{"title":"Study of the mechanisms of heteroresistance to colistin in a strain of Enterobacter cloacae by random mutagenesis.","authors":"Romane Milleville, Linda Hadjadj, Jean-Marc Rolain, Sophie Alexandra Baron","doi":"10.1016/j.jgar.2024.10.260","DOIUrl":"https://doi.org/10.1016/j.jgar.2024.10.260","url":null,"abstract":"<p><p>Enterobacter cloacae complex belong to a group for which colistin resistance is not well documented, due to their frequent heteroresistance. Previous studies have examined the role of the two-component systems PhoP-PhoQ and its negative regulator mgrB or the arnBCADTEF operon. We investigated the molecular basis of colistin heteroresistance using genome analysis and random mutagenesis in a strain of Enterobacter cloacae and identified the arnA gene as responsible for colistin resistance.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
From Forgotten Cure to Modern Medicine: The Resurgence of Bacteriophage Therapy. 从被遗忘的疗法到现代医学:噬菌体疗法的复兴。
IF 3.7 3区 医学
Journal of global antimicrobial resistance Pub Date : 2024-10-30 DOI: 10.1016/j.jgar.2024.10.259
Tasnime Abdo Ahmad, Samar El Houjeiry, Souha S Kanj, Ghassan M Matar, Esber S Saba
{"title":"From Forgotten Cure to Modern Medicine: The Resurgence of Bacteriophage Therapy.","authors":"Tasnime Abdo Ahmad, Samar El Houjeiry, Souha S Kanj, Ghassan M Matar, Esber S Saba","doi":"10.1016/j.jgar.2024.10.259","DOIUrl":"https://doi.org/10.1016/j.jgar.2024.10.259","url":null,"abstract":"<p><strong>Objectives: </strong>The unregulated use of antibiotics has led to the rise of antibiotic-resistant bacterial strains. This study explores bacteriophage therapy as an alternative treatment, highlighting its history, significance, and advancements in Europe, the USA, and the Middle East.</p><p><strong>Methods: </strong>A comprehensive literature review on bacteriophage therapy was conducted, focusing on its development, clinical trials, and patient treatment applications. The study also examined challenges, limitations, criteria for ideal phage selection, and manipulation techniques.</p><p><strong>Results: </strong>The USA and several European countries have advanced in phage therapy, progressing from clinical trials to patient treatment, while Middle Eastern countries are still in the early stages. Bacteriophages offer specificity, abundance, and minimal side effects, but challenges like safety concerns and potential resistance limit their widespread use.</p><p><strong>Conclusion: </strong>Bacteriophage therapy shows promise as an antibiotic alternative but faces safety and resistance challenges. Continued research and better regulatory frameworks, especially in the Middle East, are needed to realize its potential.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid immunochromatographic detection of carbapenemases directly from positive blood cultures in patients colonized by carbapenemase-producing bacteria. 直接从碳青霉烯酶产生菌定植患者的阳性血液培养物中快速免疫层析检测碳青霉烯酶。
IF 3.7 3区 医学
Journal of global antimicrobial resistance Pub Date : 2024-10-30 DOI: 10.1016/j.jgar.2024.10.261
Nicolas Lauwerier, Claire Duployez, Rémi Le Guern, Frédéric Wallet, Caroline Loïez
{"title":"Rapid immunochromatographic detection of carbapenemases directly from positive blood cultures in patients colonized by carbapenemase-producing bacteria.","authors":"Nicolas Lauwerier, Claire Duployez, Rémi Le Guern, Frédéric Wallet, Caroline Loïez","doi":"10.1016/j.jgar.2024.10.261","DOIUrl":"https://doi.org/10.1016/j.jgar.2024.10.261","url":null,"abstract":"<p><strong>Objectives: </strong>Carbapenem resistance is increasing worldwide. Earlier detection of this resistance, combined with appropriate treatment, could improve the prognosis of bloodstream infection. This study aims to evaluate the detection of carbapenemase producing gram-negative bacteria directly from positive blood cultures to quickly adapt antibiotic therapy before the results of antibiotic susceptibility testing are available.</p><p><strong>Method: </strong>A prospective single-center study was conducted over a five-month period at Lille University Hospital. Carbapenemase detection by immunochromatographic testing was performed directly from positive blood cultures with gram-negative rods of thirty-five patients previously colonized with carbapenemase-producing bacteria.</p><p><strong>Results: </strong>Among these thirty-five positive blood cultures, 15 carbapenemase-producing strains were directly detected, mainly OXA-48 and NDM. This rapid procedure provided results in less than one hour, compared to several hours for conventional methods. Of the patients with infections caused by carbapenemase-producing isolates, 67% (10 patients) received inappropriate empiric treatment, highlighting the potential of the rapid test to adjust antibiotic therapy sooner.</p><p><strong>Conclusions: </strong>Carbapenemase detection by immunochromatographic testing directly on blood culture pellets is reliable and can lead to early adaptation of antibiotic therapy in these severe infections.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integral genomic description of blaNDM-5-harbouring plasmids recovered from Enterobacterales in Argentina. 从阿根廷肠杆菌中回收的 blaNDM-5-harbouring 质粒的整体基因组描述。
IF 3.7 3区 医学
Journal of global antimicrobial resistance Pub Date : 2024-10-28 DOI: 10.1016/j.jgar.2024.10.258
González-Espinosa Francisco, Di Pilato Vincenzo, Calabrese Luca, Costa Elisa, Costa Agustina, Gutkind Gabriel, Cejas Daniela, Radice Marcela
{"title":"Integral genomic description of bla<sub>NDM-5</sub>-harbouring plasmids recovered from Enterobacterales in Argentina.","authors":"González-Espinosa Francisco, Di Pilato Vincenzo, Calabrese Luca, Costa Elisa, Costa Agustina, Gutkind Gabriel, Cejas Daniela, Radice Marcela","doi":"10.1016/j.jgar.2024.10.258","DOIUrl":"https://doi.org/10.1016/j.jgar.2024.10.258","url":null,"abstract":"<p><strong>Objective: </strong>To characterise four bla<sub>NDM-5</sub>-harbouring plasmids recovered in Enterobacterales isolated in Argentina.</p><p><strong>Methods: </strong>DNA was sequenced by Illumina and Oxford Nanopore technologies, assembled using Unicycler, analysed using PlasmidFinder, MOB-Typer, IslandViewer4 and Resfinder, and visualised by Proksee and Clinker. bla<sub>NDM-5</sub>-harbouring plasmids were compared with deposited similar ones using PLSDB.</p><p><strong>Results: </strong>two plasmids belonged to incompatibility group IncFII where bla<sub>NDM-5</sub> was located in a previously described genetic context, immersed in an antimicrobial resistance island (ARI). Local IncFII plasmids displayed high similarity (≥ 90% shared hashes (sh)) with four deposited in PLSDB. The other two local plasmids belonged to the multi-replicon group IncFIB-HI1B, harbouring bla<sub>NDM-5</sub> in a novel variant of the genetic context. Both multi-replicon plasmids, presented two ARI, one containing bla<sub>NDM-5</sub> in addition to other ARM; and the second ARI carrying bla<sub>CTX-M-15</sub> and a class 1 integron. Plasmids deposited in PLSDB showed low similarity to local multi-replicon plasmids. The most similar plasmids (n:5) displayed less than 60% sh showing the same Inc groups but lacking ARM.</p><p><strong>Conclusion: </strong>This study broadens the limited understanding of bla<sub>NDM-5</sub>-harbouring plasmids in Latin America. Furthermore, it represents the first description of bla<sub>NDM-5</sub> in a novel variant of the common genetic platform and its location in multi-replicon IncFIB-IncHI1B plasmids, which were not previously associated with any antimicrobial resistance marker (ARM).</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exposure factors associated with antimicrobial resistance and identification of management practices for preharvest mitigation along the broiler production systems: a systematic review. 与抗菌素耐药性相关的暴露因素以及确定肉鸡生产系统收获前缓解抗菌素耐药性的管理方法:系统综述。
IF 3.7 3区 医学
Journal of global antimicrobial resistance Pub Date : 2024-10-25 DOI: 10.1016/j.jgar.2024.09.010
Yasir R Khan, Jorge A Hernandez, Subhashinie Kariyawasam, Garry Butcher, Daniel M Czyz, Abraham J Pellissery, Thomas Denagamage
{"title":"Exposure factors associated with antimicrobial resistance and identification of management practices for preharvest mitigation along the broiler production systems: a systematic review.","authors":"Yasir R Khan, Jorge A Hernandez, Subhashinie Kariyawasam, Garry Butcher, Daniel M Czyz, Abraham J Pellissery, Thomas Denagamage","doi":"10.1016/j.jgar.2024.09.010","DOIUrl":"https://doi.org/10.1016/j.jgar.2024.09.010","url":null,"abstract":"<p><strong>Objectives: </strong>This systematic review aims to [1] determine the risk of AMR development associated with AMU and other exposure factors in broiler, and [2] identify the best management practices to mitigate preharvest AMR development in enteric bacteria along the broiler production.</p><p><strong>Methods: </strong>Study selection criteria was PECO/PICO framework and included broiler (population), AMU or other management practices (exposure or intervention), organic or antibiotic-free production (comparator), and presence of AMR-enteric bacteria/genes (outcome). Peer-reviewed primary research studies were searched in PubMed on December 19, 2022, and AGRICOLA, Embase, Scopus, and Web of Science on February 10, 2023. The risk of bias in studies was assessed using modified ROBIS-E risk of bias assessment tool. Results were synthesized and presented narratively according to PRISMA-2020 guidelines.</p><p><strong>Results: </strong>205/2699 studies were subjected to full-text review, and fifteen included in the final synthesis. Enteric bacteria Escherichia coli, Salmonella and Campylobacter exhibited AMR and multidrug resistance (MDR) against several critically important antimicrobials (aminoglycoside, cephalosporin, chloramphenicol, macrolide, penicillin, quinolone, tetracycline, and sulfonamide) for human health. The risk of AMR development in bacteria is potentially higher with AMU in broiler production. Substandard farm management practices, poor biosecurity measures, and conventional production system are also associated with dissemination of AMR in bacteria.</p><p><strong>Conclusion: </strong>Findings indicate, AMU exposure is associated with considerably higher risks of AMR development in enteric bacteria. Antimicrobial stewardship, organic/antibiotic-free broiler production, good farm management practices, and high-level biosecurity measures can substantially mitigate preharvest AMR development in enteric bacteria. However, most of the studies are cross-sectional and therefore, causal inference cannot be established.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome characteristics of a MDR Pseudomonas monteilii carrying a novel VIM-type β-lactamase, blaVIM-84. 携带新型 VIM 型β-内酰胺酶 blaVIM-84 的 MDR 蒙蒂利假单胞菌的基因组特征。
IF 3.7 3区 医学
Journal of global antimicrobial resistance Pub Date : 2024-10-22 DOI: 10.1016/j.jgar.2024.09.007
Jinjing Tu, Yi Liu, Wenjing Xu, Xiaoqi Dong, Liang Zhang, Jiajie Qian, Shuguang Xu
{"title":"Genome characteristics of a MDR Pseudomonas monteilii carrying a novel VIM-type β-lactamase, bla<sub>VIM-84</sub>.","authors":"Jinjing Tu, Yi Liu, Wenjing Xu, Xiaoqi Dong, Liang Zhang, Jiajie Qian, Shuguang Xu","doi":"10.1016/j.jgar.2024.09.007","DOIUrl":"https://doi.org/10.1016/j.jgar.2024.09.007","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to determine the genetic environment and characterize plasmid carrying a novel VIM-type β-lactamase (VIM-84) in a multidrug-resistant Pseudomonas monteilii isolate obtained from the human gut through whole-genome sequencing.</p><p><strong>Methods: </strong>DNA extraction of P. monteilii L2757hy was performed using the Genomic DNA Isolation Kit (QIAGEN, Hilden, Germany). Whole-genome sequencing was performed by Illumina NovaSeq 6000 and Oxford Nanopore platforms. The transferability of resistance genes was screened single clonal on MHA plates containing rifampicin and meropenem. Verification was performed using MALDI/TOF-MS and PCR with Pseudomonas aeruginosa PAO1Ri as the recipient strain.</p><p><strong>Results: </strong>L2757hy was identified as P. monteilii through sequencing and ANI analysis. The genome was assigned as ST147 and comprised a 6,130,057 bp chromosome with a GC content of 61.8% and a 49,704 bp plasmid. Several resistance genes, including bla<sub>IMP-1</sub>, aac(6')-IIa and tmexCD-toprJ, as well as virulence genes such as iroN, and wzaJ, were identified on the chromosome. A novel VIM-type bla<sub>VIM-84</sub> was found on the plasmid, which was previously identified in Pseudomonas aeruginosa. Plasmid harboring bla<sub>VIM-84</sub> was untypable, and it could be transferred to P. aeruginosa PAO1Ri and was associated with a class I integron with the genetic environment intI1-bla<sub>VIM-84</sub>-tniR-tniQ-tniB-tniA, likely derived from Tn402.</p><p><strong>Conclusions: </strong>Our study revealed that the novel bla<sub>VIM-84</sub> gene was harbored by P. monteilii rather than P. aeruginosa. We suggested that P. monteilii may serve as a reservoir for resistance genes.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome sequence of a ST65 hypervirulent Klebsiella pneumoniae isolate carrying mcr-8 from China. 中国一个携带 mcr-8 的 ST65 型高病毒性肺炎克雷伯氏菌分离株的基因组序列。
IF 3.7 3区 医学
Journal of global antimicrobial resistance Pub Date : 2024-10-19 DOI: 10.1016/j.jgar.2024.09.006
Weili Zhang, Hao Xu, Yaling Li, Ruishan Liu, Danfeng Lou
{"title":"Genome sequence of a ST65 hypervirulent Klebsiella pneumoniae isolate carrying mcr-8 from China.","authors":"Weili Zhang, Hao Xu, Yaling Li, Ruishan Liu, Danfeng Lou","doi":"10.1016/j.jgar.2024.09.006","DOIUrl":"https://doi.org/10.1016/j.jgar.2024.09.006","url":null,"abstract":"<p><strong>Objectives: </strong>In this study, we report the complete genome sequence of a ST65 hypervirulent Klebsiella pneumoniae isolate carrying mcr-8 from China. The aim was to investigate its molecular characteristics and resistant mechanism.</p><p><strong>Methods: </strong>A colistin-resistant hvKP was isolated from an inpatient in China. The whole genome was sequenced on Illumina NovaSeq 6000 and long-read ONT platforms. de novo assembly was conducted using SPAdes and Unicycler. S1 nuclease pulsed-field gel electrophoresis, Southern-blot, and antimicrobial susceptibility testing were performed. Sequence type, antimicrobial resistance and virulence-related genes were predicted from the sequence. The circular maps of multiple plasmids comparisons were drawn by the BLAST Ring Image.</p><p><strong>Results: </strong>The complete genome sequence of K. pneumoniae ACESH00926 consists of one chromosome and two plasmids. ACESH00926 belongs to K2 ST65 according to the MLST scheme. ACESH00926 showed high resistance to colistin (MIC > 8 µg/mL). Several ARGs were identified, including mobile colistin-resistant gene mcr-8 which was located in an IncFIl(K)/IncFIA(HI1) type plasmid. The bigger plasmid was a pK244-like virulence plasmid. It carrying a series of virulence genes, such as the regulator of the mucoid phenotype (rmpA and rmpA2), salmochelin siderophore biosynthesis (iroB), ABC transporter (iroC), ferric aerobactin receptor (iutA), aerobactin siderophore biosynthesis protein (iucC), and aerobactin synthetase (iucA) encoded genes. And another plasmid carrying mcr-8 with a conserved genetic context (dgkA-sasA-copR-mcr-8-ccdA-ccdB-xerD-repE-parM-umuC-lexA-klcA).</p><p><strong>Conclusions: </strong>It is necessary to emphasize the necessity of monitoring a combination of colistin-resistant and hypervirulent Klebsiella pneumoniae strains in the future.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The prevalence of carbapenem-resistant Enterobacterales and the emergence of novel ST11-KL30 carbapenem-resistant Klebsiella pneumoniae in Xinjiang, China 中国新疆耐碳青霉烯类肠杆菌的流行和新型 ST11-KL30 耐碳青霉烯类肺炎克雷伯菌的出现。
IF 3.7 3区 医学
Journal of global antimicrobial resistance Pub Date : 2024-10-18 DOI: 10.1016/j.jgar.2024.10.001
{"title":"The prevalence of carbapenem-resistant Enterobacterales and the emergence of novel ST11-KL30 carbapenem-resistant Klebsiella pneumoniae in Xinjiang, China","authors":"","doi":"10.1016/j.jgar.2024.10.001","DOIUrl":"10.1016/j.jgar.2024.10.001","url":null,"abstract":"<div><h3>Objectives</h3><div>To address the lack of research on the prevalence of carbapenem-resistant Enterobacterales (CREs) in Xinjiang, China, and elucidate the genomic characteristics of carbapenem-resistant <em>Klebsiella pneumoniae</em> (CRKP) ST11-KL30.</div></div><div><h3>Methods</h3><div>CREs were collected in Xinjiang from 2021 to 2023. The antimicrobial susceptibility testing of carbapenems was performed via agar dilution method. Whole-genome sequencing was completed on the Illumina platform, and subsequent genomic analyses of CRKP, such as sequencing typing, K-locus and O-locus identification, virulence score assessment, and phylogenetic analysis, were performed. The virulence of CRKP isolates was determined <em>in vitro</em> and <em>in vivo</em>, and biofilm formation was assessed by crystal violet staining. Additionally, the virulence plasmid was reconstructed, and the formation of CRKP ST11-KL30 was revealed based on genome data from public database.</div></div><div><h3>Results</h3><div>Eighty-five CRE isolates were collected, among which CRKP was most prevalent (68/85). KPC was the most dominant carbapenemase (60/68) in CRKP, while NDM-type carbapenemase was more prevalent in other species. ST11 was the dominant CRKP clone and was phylogenetically divided into three clusters: ST11-KL64, ST11-KL47 and ST11-KL30. CRKP ST11-KL30 is a novel recombinant clone that harbours a pK2044-like virulence plasmid and can be derived from ST11-KL64 by obtaining an ∼57 kb region from ST29-KL30. Compared to ST11-KL47 and ST11-KL64, ST11-KL30 had lower virulence, but had enhanced biofilm formation.</div></div><div><h3>Conclusions</h3><div>We describe the prevalence of CRE prevalence southern Xinjiang and report the emergence of a region-specific clone. Our findings underscore the potential dissemination of ST11-KL30, which warrants increased monitoring in the future.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of carbapenem-resistant gram-negative bacilli in Japan using the fully automated bacterial testing device RAISUS S4. 使用全自动细菌检测设备 RAISUS S4 检测日本耐碳青霉烯革兰阴性杆菌。
IF 3.7 3区 医学
Journal of global antimicrobial resistance Pub Date : 2024-10-18 DOI: 10.1016/j.jgar.2024.09.009
Yumiko Funashima, Rin Hamabe, Kei Tominaga, Kentaro Wakamatsu, Takahiro Yaguchi, Zenzo Nagasawa, Tsukuru Umemura
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