Journal of global antimicrobial resistance最新文献

{"title":"Genomic dissection of nosocomial multiclonal-outbreak by carbapenem-resistant Pseudomonas aeruginosa strains in a tertiary children’s hospital in Japan","authors":"Akihiko Shimizu ,&nbsp;Jun Kurushima ,&nbsp;Koichi Tanimoto ,&nbsp;Naoko Tomie ,&nbsp;Yusuke Hashimoto ,&nbsp;Takahiro Nomura ,&nbsp;Natsuko Ota ,&nbsp;Hidetada Hirakawa ,&nbsp;Haruyoshi Tomita","doi":"10.1016/j.jgar.2025.05.026","DOIUrl":"10.1016/j.jgar.2025.05.026","url":null,"abstract":"<div><h3>Objective</h3><div>Carbapenem-resistant <em>Pseudomonas aeruginosa</em> (CRPA) is an important antimicrobial-resistant bacterial pathogen. We observed an increase in the detection rate of CRPA isolates at a tertiary children’s hospital in Japan. We suspected an outbreak of a clonal CRPA strain and conducted a genetic study to elucidate the resistance mechanism and possible spread of CRPA strains.</div></div><div><h3>Methods</h3><div>For 25 CRPA isolates, antimicrobial susceptibilities to eight antibiotics that are generally used for <em>P. aeruginosa</em> infection were tested. Whole genomes of the isolates were sequenced via next-generation sequencing analysis and subjected to comparative lineage analysis, AMR gene profiling, and Bayesian inference of transmission routes.</div></div><div><h3>Results</h3><div>The genomes of the strains were not monoclonal but comprised multiple lineages with various sequence types. The CRPA isolates had a dysfunctional <em>oprD</em> gene, which is an importer of carbapenem; however, specific nucleotide mutations varied by lineage. Furthermore, administration of antimicrobials against CRPA, such as piperacillin, ceftazidime, and aztreonam, was immediately followed by resistance to these antimicrobials, and in these cases, some unique single nucleotide variants in lasR were identified.</div></div><div><h3>Conclusions</h3><div>Molecular epidemiological data have revealed a multilineage outbreak of <em>P. aeruginosa</em> strains that independently developed carbapenem resistance. This emphasises the rapid evolution of antimicrobial resistance in <em>P. aeruginosa</em> in response to the antimicrobial doses administered to patients who are hospitalised.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 89-94"},"PeriodicalIF":3.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urinary tract infections caused by Gram-negative bacteria in elderly hospitalized patients: Epidemiology, clinical features and outcomes in the era of antimicrobial resistance","authors":"Gabriele Giuliano ,&nbsp;Giulia Hankache ,&nbsp;Margherita Sambo ,&nbsp;Maria Grazia Cusi ,&nbsp;Pietro Enea Lazzerini ,&nbsp;Luigi Gennari ,&nbsp;Massimiliano Fabbiani ,&nbsp;Francesca Montagnani ,&nbsp;Mario Tumbarello","doi":"10.1016/j.jgar.2025.06.006","DOIUrl":"10.1016/j.jgar.2025.06.006","url":null,"abstract":"<div><h3>Objective</h3><div>Urinary tract infections (UTI) are among the most common infections in elderly hospitalized patients, often caused by multi-drug resistant (MDR) organisms and characterized by poor clinical outcomes. This study aimed to evaluate the clinical characteristics, microbiology, treatment patterns, and predictors of mortality in elderly hospitalized patients with UTI, with a focus on infections caused by MDR Gram-negative bacteria (MDR-GNB).</div></div><div><h3>Methods</h3><div>A retrospective analysis was conducted on 171 patients hospitalized between January 2022 and December 2023 at a large academic hospital in Siena, Italy. Data on demographics, comorbidities, infection characteristics, antimicrobial resistance, treatments, and outcomes were collected. Risk factors for MDR-GNB UTI and predictors of 14-d mortality were identified through univariable and multivariable analyses.</div></div><div><h3>Results</h3><div>Of 171 patients (median age: 82 y), 106 (62.0%) had a catheter-associated UTI, and 105 (61.4%) had a healthcare-associated UTI. MDR-GNB were isolated in 68.4% of cases. Common pathogens included <em>Escherichia coli</em> (74/171, 43.3%), <em>Klebsiella</em> spp. (39/171, 22.8%) and <em>Pseudomonas aeruginosa</em> (33/171, 19.3%). Among Enterobacterales 35.1% were Extended-spectrum β-lactamases-producers, 3.7% carried <em>Klebsiella pneumoniae</em> carbapenemase, and 3.0% metallo-β-lactamases. Overall, the 14-d mortality rate was 12.9%. Predictors of 14-d mortality included septic shock, infections caused by <em>Providencia stuartii,</em> infections caused by New Delhi metallo-β-lactamase-producing <em>K. pneumoniae</em>, and inappropriate empirical antibiotic therapy.</div></div><div><h3>Conclusions</h3><div>UTI significantly affect hospital length of stay and mortality in elderly patients. In the current context, resistance mechanisms including <em>K. pneumoniae</em> carbapenemase and metallo-β-lactamases production, must be considered when managing these infections. Prompt recognition of risk factors for infections caused by MDR organisms and optimized antimicrobial strategies are essential to improve outcomes in this vulnerable population.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 116-126"},"PeriodicalIF":3.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights of the co-existence of blaOXA-23, blaOXA-91, blaNDM-1 harboring carbapenem-resistant Acinetobacter Baumannii isolates from the intensive care units environment in Shanghai","authors":"Xiao Wang ,&nbsp;Bing Zhao ,&nbsp;Yuqing Zhou ,&nbsp;Yue Zhang ,&nbsp;Tongsheng Xu ,&nbsp;Yuan Zhuang ,&nbsp;Min Chen ,&nbsp;Lipeng Hao ,&nbsp;Yifeng Shen ,&nbsp;Jun Feng","doi":"10.1016/j.jgar.2025.05.025","DOIUrl":"10.1016/j.jgar.2025.05.025","url":null,"abstract":"<div><h3>Objectives</h3><div>This study assessed the transmission, prevalence and genomic characteristics of carbapenem-resistant <em>Acinetobacter Baumannii</em> (CARB) isolated from object surfaces and healthcare workers' hands in seven hospitals of Shanghai in 2019 (pre-COVID pandemic) and 2023 (post-COVID pandemic).</div></div><div><h3>Methods</h3><div>CRAB isolates were tested for antimicrobial susceptibility using the broth microdilution method and whole-genome sequencing. Antimicrobial resistance genes, Multilocus Sequence Typing (MLST), polysaccharide capsule and plasmid migration were evaluated based on tools such as VFDB database, PubMLST, Kaptive, MOB-suite, etc.</div></div><div><h3>Results</h3><div>Of 3,156 samples collected, 130 strains of CRAB were isolated. The prevalence of CRAB in the ICU setting was significantly lower in 2023 (2.44%) than in 2019 (5.60%). Objects that come into direct contact with patients served as a primary source (43.85%). CRAB isolates exhibited higher resistance to β-lactamase antibiotics but lower to minocycline (2.31%), tigecycline (3.08%) and polymyxin B (2.31%). Five ST_Pasteur and eleven ST_Oxford were identified, predominantly ST2_Pasteur (93.6%) and ST208_Oxford (40%). Two novel sequence types (STs) named ST3329 and ST3331 were found in the surface of objects such as door handles. Notably, two CARB isolates (designated 2023-AB023 and 2023-AB033) co-harboring the <em>bla</em>_OXA-23, <em>bla</em>_OXA-91, <em>bla</em>_NDM-1 were found to be high genomic similarity to DETAB-R21 previously isolated from an ICU patient's oral swab with high environmental adaptability, reported in August 2022 in Zhejiang Province of China.</div></div><div><h3>Conclusions</h3><div>The resistance rate of CRAB was slightly lower in 2023 compared to 2019. However, the emergence of pan-drug-resistant CRAB, along with the coexistence of carbapenemase-producing strains (OXA-23, OXA-51-like, and NDM-1) in 2023, underscore the dynamic progression of antimicrobial resistance (AMR) in this pathogen. These findings suggest a potential epidemiological shift characterized by clonal diversification alongside persistent carbapenemase-driven resistance mechanisms.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 72-80"},"PeriodicalIF":3.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and genomic characterization of Enterobacter kobei ST56 co-carrying mcr-9, blaNDM-1, and blaCTX−M-3 resistance genes","authors":"Bingyou Yin ,&nbsp;Ruishan Liu ,&nbsp;Xinjun Hu","doi":"10.1016/j.jgar.2025.06.001","DOIUrl":"10.1016/j.jgar.2025.06.001","url":null,"abstract":"<div><h3>Objectives</h3><div>This study reports the complete genome sequence of <em>Enterobacter kobei</em> (<em>E. kobei</em>) L5089hy of ST56 isolated from China, carrying <em>mcr-9, bla</em>_NDM-1, and <em>bla</em>_CTX−M-3. It aims to investigate its molecular characteristics and antibiotic resistance mechanisms.</div></div><div><h3>Methods</h3><div>The <em>E. kobei</em> strain was isolated from the faecal sample of an in-patient in a hospital in Hangzhou, China. Whole-genome sequencing was performed using Oxford Nanopore technology, and the genome was assembled with Unicycler version 0.4.7. The strain was identified by 16S rRNA sequencing. The genome sequence was uploaded to ResFinder version 4.1 to identify antimicrobial resistance genes (ARGs). Multilocus sequence typing (MLST) was used to determine the sequence type of the strain. Genome annotation and plasmid type identification were carried out with the aid of the Proksee and PlasmidFinder databases. An antimicrobial susceptibility test (AST) was conducted on the strain. Circular mapping of multiple plasmid comparisons was drawn by BLAST Ring Image.</div></div><div><h3>Results</h3><div><em>E. kobei</em> L5089hy consists of 1 chromosome and 5 plasmids. The <em>mcr-9</em> gene is located on the chromosome, <em>bla</em>_NDM-1 is on the IncFII(Yp) plasmid, and <em>bla</em>_CTX−M-3 is present on both the chromosome and the IncN2 plasmid. The MLST typing result shows that L5089hy is of the ST56 type. AST indicates that the strain is resistant to a variety of antibiotics. However, despite carrying the <em>mcr-9</em> gene, it is sensitive to colistin (minimum inhibitory concentration = 1 μg/mL).</div></div><div><h3>Conclusions</h3><div>This study emphasizes the necessity for monitoring <em>E. kobei</em>© 2025 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 186-188"},"PeriodicalIF":3.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic similarity of carbapenem-resistant Enterobacterales collected from mothers and their neonates","authors":"Priyanka Basak ,&nbsp;Sharmi Naha ,&nbsp;Kirsty Sands ,&nbsp;Subhajit Dutta ,&nbsp;Suchandra Mukherjee ,&nbsp;Bijan Saha ,&nbsp;Timothy R. Walsh ,&nbsp;Sulagna Basu","doi":"10.1016/j.jgar.2025.06.004","DOIUrl":"10.1016/j.jgar.2025.06.004","url":null,"abstract":"<div><h3>Objective</h3><div>New Delhi metallo-β-lactamase is endemic in India and the gut may act as a reservoir of carbapenemase-producing Enterobacterales (CPE). Maternal gut colonisation with <em>bla</em>_NDM-harbouring CPE increases the risk of neonatal gut colonisation. This study aimed to assess the vertical transmission of CPE from pregnant mothers (rectal) to neonates (rectal and blood).</div></div><div><h3>Methods</h3><div>Rectal samples were collected and processed for the presence of CPE, followed by bacterial identification and antibiotic susceptibility. Mother–neonate pairs colonised with the same species underwent pulsed-field gel electrophoresis and whole-genome sequencing to examine genetic relatedness. Detection of <em>bla</em>_NDM variants and their transmissibility was performed.</div></div><div><h3>Results</h3><div>Of the pregnant mothers (<em>n</em> = 86) and sick neonates (<em>n</em> = 93) analysed, eight mother–neonate pairs harboured similar carbapenem-resistant species, predominantly <em>Klebsiella pneumoniae</em>, followed by <em>Escherichia coli</em>. Pulsed-field gel electrophoresis and whole-genome sequencing revealed that most isolates from mother–neonate pairs were distinct and distributed within diverse sequence types, including epidemic clones (ST11/15/147/405/410). <em>bla</em>_NDM-1/5/7 were detected in CPE and predominantly associated with conjugative IncFII and IncFII(K) replicons. Genomic analysis supported one case of vertical transmission (ST147; <em>bla</em>_NDM-1-positive <em>K. pneumoniae</em>) from mother to a neonate. Further investigation of exogenous sources is required to understand the acquisition of bacteria. No evidence of transmission of <em>bla</em>_NDM-harbouring plasmids within mother–neonate pairs carrying distinct isolates was observed, indicating the independent acquisition of bacteria.</div></div><div><h3>Conclusions</h3><div>Although limited evidence of mother-to-neonate transmission was observed in this study, screening of the gut is necessary to understand CPE transmission in hospital settings and beyond. Targeted surveillance and infection-prevention policies are needed to curb CPE spread.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 166-179"},"PeriodicalIF":3.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activity of aztreonam-avibactam and ceftazidime-avibactam against β-lactamase-producing enterobacterales Isolates from United States hospitals","authors":"Lalitagauri M Deshpande,&nbsp;Timothy B Doyle,&nbsp;Helio S Sader,&nbsp;Mariana Castanheira","doi":"10.1016/j.jgar.2025.06.002","DOIUrl":"10.1016/j.jgar.2025.06.002","url":null,"abstract":"<div><h3>Objectives</h3><div>Enterobacterales isolates producing β-lactamases are widespread and threaten the use of β-lactams. This study evaluated the activity of aztreonam-avibactam and comparator antimicrobial agents against Enterobacterales isolates producing common β-lactamases collected in US hospitals.</div></div><div><h3>Methods</h3><div>A total of 18,148 Enterobacterales isolates collected during 2020–2021 in US hospitals (<em>n</em> = 71) were susceptibility tested by reference broth microdilution methods. A total of 2,337 isolates were submitted to whole genome sequencing due to elevated cephalosporins/aztreonam MIC values or carbapenem nonsusceptibility (meropenem and/or imipenem MIC results at &gt;1 mg/L).</div></div><div><h3>Results</h3><div>ESBL enzymes were observed among 1,430 carbapenem-susceptible <em>E. coli, K. pneumoniae, E. cloacae</em> and <em>Citrobacter</em> spp. isolates and CTX-M-15 was the most common ESBL. Ceftazidime-avibactam inhibited all ESBL-producers while ceftolozane-tazobactam inhibited 68.4–95.9%. Aztreonam-avibactam inhibited &gt;99.7% of the isolates regardless of the ESBL type or organism. Among other classes, amikacin and tigecycline were the most active agents, inhibiting 78.5% and 97.8% of the ESBL-producing isolates. All isolates carrying transferrable AmpC genes were susceptible to ceftazidime-avibactam and meropenem-vaborbactam, and 98.1% susceptible to aztreonam-avibactam, but only 83.3% were susceptible to ceftolozane-tazobactam. Five isolates carried <em>bla</em>_CMY-42 with a PBP3 insertion and they exhibited aztreonam-avibactam MICs ranging from 2 to 16 mg/L. Among carbapenemase-producers (<em>n</em> = 157), aztreonam-avibactam, ceftazidime-avibactam and meropenem-vaborbactam susceptibility rates were 98.7%, 81.5% and 79.6%. The only comparator displaying activity against these isolates was tigecycline (93.0% susceptible).</div></div><div><h3>Conclusions</h3><div>New β-lactam/β-lactamase inhibitors were active against common β-lactamase-producing isolates from US hospitals, including carbapenemase-producing isolates for which therapeutic options are limited. Aztreonam-avibactam was the most active agent against carbapenemase producers, including MBL-carrying isolates.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 103-110"},"PeriodicalIF":3.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of genomic islands involved in antimicrobial resistance in Proteus mirabilis isolated from swine","authors":"Hui Su ,&nbsp;Lirong Yang ,&nbsp;Mimi Liu ,&nbsp;Xiaorui Pan ,&nbsp;Hannuo Li ,&nbsp;Wanli Sha ,&nbsp;Baishuang Yin ,&nbsp;Wenlong Dong ,&nbsp;Guojiang Li","doi":"10.1016/j.jgar.2025.06.003","DOIUrl":"10.1016/j.jgar.2025.06.003","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Proteus mirabilis</em> (<em>P. mirabilis</em>), a Gram-negative, rod-shaped bacterium, is ubiquitously present in natural environments as well as within the intestinal tracts of humans and animals. We isolated <em>P. mirabilis</em> SH-4 from a swine sample and investigated its antibiotic resistance genes (ARGs) along with their genetic structure.</div></div><div><h3>Methods</h3><div>In this study, we have performed antimicrobial susceptibility testing by the broth microdilution method and conducted a comprehensive analysis through whole genome sequencing (WGS) and phylogenomics of <em>P. mirabilis</em> strain SH-4.</div></div><div><h3>Results</h3><div><em>P. mirabilis</em> SH-4 was found to possess a single chromosome measuring 4 123 479 base pairs in length, with a GC content of 39.06%. Besides, <em>P. mirabilis</em> SH-4 exhibited resistance to eleven antimicrobial agents tested. Through single nucleotide polymorphism (SNP) profiling, we revealed the genetic relationship of SH-4 with other <em>P. mirabilis</em> strains carrying various ARGs. WGS analysis revealed that SH-4 harbored a ΔTn<em>7</em> transposon and two distinct genomic islands (GIs), designated as PmGRI-9-SH-4 and PmGRI-10-SH-4. ΔTn<em>7</em> was identified, corresponding to Tn<em>7</em> with an insertion of IS<em>256</em> within its sequence. PmGRI-9-SH-4 displayed partial homology to PmGRI1-STP3 and PmGRI1-YN9 (China:Sichuan). Additionally, PmGRI-10-SH-4 contained composite transposons and eleven Type IV secretion system (T4SS) related genes, including <em>traN, traU, traC</em>, and others, with the MDR region situated near the terminus of this GI.</div></div><div><h3>Conclusions</h3><div>In summary, GIs and ISs may serve as crucial facilitators in the acquisition of resistance genes in <em>P. mirabilis</em>. These genetic elements turn <em>P. mirabilis</em> into highly resistant bacteria, culminating in infections that pose significant therapeutic challenges.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 160-165"},"PeriodicalIF":3.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome sequencing of coexisting blaNDM-1, blaKPC-2, and mcr-9.1 in a clinical carbapenem-resistant Citrobacter braakii isolate","authors":"Fengqiang Sun ,&nbsp;Xiaomei Yu ,&nbsp;Fengli Zhang ,&nbsp;Jie Ma ,&nbsp;Ping Ji","doi":"10.1016/j.jgar.2025.05.027","DOIUrl":"10.1016/j.jgar.2025.05.027","url":null,"abstract":"<div><h3>Objective</h3><div>We aimed to characterize the molecular characteristics and clinical features of carbapenem-resistant <em>Citrobacter braakii</em> carrying <em>bla</em>_NDM-1, <em>bla</em>_KPC-2, and <em>mcr-9.1</em> using whole-genome sequencing.</div></div><div><h3>Methods</h3><div>The susceptibility of <em>C. braakii</em> (WF0082) to 22 antimicrobial drugs was tested by an automated microbiology system. Whole-genome sequencing was performed using the Illumina and Nanopore platforms. Resistance genes and plasmid replicon types were determined. Gene structure was compared between strains to analyse the plasmid properties and gene environments of carbapenem (<em>bla</em>_NDM-1 and <em>bla</em>_KPC-2) and mobilized colistin resistance genes (<em>mcr-9.1</em>). The core single-nucleotide polymorphisms were used to construct an evolutionary tree.</div></div><div><h3>Results</h3><div>WF0082 was resistant to all antibiotics excluding colistin. <em>bla</em>_NDM-1 was located on the truncated transposon Tn<em>125</em> in the IncX3 plasmid. <em>bla</em>_KPC-2 was located in the IncP6/IncX6 plasmid, a novel conjugative plasmid whose core <em>bla</em>_KPC platform (IS<em>Kpn27</em>-<em>bla</em>_TEM-1-<em>bla</em>_KPC-2-ΔIS<em>Kpn6</em>-<em>korC</em>-<em>klcA</em>-<em>orf279</em>-<em>orf396</em>-Δ<em>repB</em>) was derived mainly from the transposon Tn<em>6296</em>. The <em>mcr-9.1</em> core region consisted of <em>rcnR</em>-<em>rcnA</em>-<em>pcoE</em>-Δ<em>pcoS</em>-IS<em>903B</em>-<em>mcr-9.1</em>-<em>wbuC</em>-IS<em>26</em>, and it was similar to the Tn<em>6725</em> remnant. Based on phylogenetic analysis, the present strain was categorized into the C1 clade, and it was highly homologous to <em>C. braakii</em> isolates from Australia and China.</div></div><div><h3>Conclusions</h3><div>The results of this study showed that <em>C. braakii</em> strain WF0082 carrying <em>bla</em>_NDM-1, <em>bla</em>_KPC-2, and <em>mcr-9.1</em> located on three plasmids was highly resistant to antimicrobial drugs. The ability of <em>C. braakii</em> to easily integrate foreign genes has the potential to seriously undermine clinical therapy, and it is urgent that the surveillance of strains harbouring multiple resistant genes is strengthened.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 95-102"},"PeriodicalIF":3.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidence of circulation of Klebsiella pneumoniae isolates among termites, chimpanzees, and humans","authors":"Cheikh Tidiane Houmenou ,&nbsp;Habibou Sarr ,&nbsp;Seydina M. Diene ,&nbsp;Idir Kacel ,&nbsp;Cheikh Sokhna ,&nbsp;Florence Fenollar ,&nbsp;Oleg Mediannikov","doi":"10.1016/j.jgar.2025.05.028","DOIUrl":"10.1016/j.jgar.2025.05.028","url":null,"abstract":"","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 69-71"},"PeriodicalIF":3.7,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"blaNDM-5-encoding ST171 Enterobacter hormaechei: A global genomic and epidemiological perspective","authors":"Mengting Luo ,&nbsp;Yawen Zhang ,&nbsp;Fang He ,&nbsp;Zhengquan Yang","doi":"10.1016/j.jgar.2025.05.024","DOIUrl":"10.1016/j.jgar.2025.05.024","url":null,"abstract":"<div><h3>Objective</h3><div>Six <em>bla</em>_NDM-5-positive carbapenem-resistant <em>Enterobacter hormaechei</em> isolates were collected from the emergency intensive care unit of a tertiary hospital in China. This study aimed to conduct a comprehensive genomic and epidemiological analysis of ST171 <em>E. hormaechei</em> harbouring <em>bla</em>_NDM-5 on a global scale.</div></div><div><h3>Methods</h3><div>Whole-genome sequencing was performed using the Illumina platform, and the genomic characteristics of the isolates were analysed using multiple bioinformatics tools. <em>E. hormaechei</em> strains carrying <em>bla</em>_NDM-5 were retrieved from the National Center for Biotechnology Information database. A single nucleotide polymorphism-based phylogenetic tree for ST171 <em>E. hormaechei</em> strains was constructed using Snippy v4.6.0.</div></div><div><h3>Results</h3><div>Six ST171 <em>E. hormaechei</em> strains carrying <em>bla</em>_NDM-5 were isolated from different patients in the EICU over a 9-month period. These strains exhibited resistance to all tested antimicrobial agents except for sulfamethoxazole/trimethoprim and colistin. A total of 12 antimicrobial resistance genes, including <em>bla</em>_NDM-5, were identified in these strains. Phylogenetic analysis revealed that all six strains belonged to the same clonal lineage, indicating nosocomial transmission. A comprehensive search of the National Center for Biotechnology Information database identified 75 ST171 <em>E. hormaechei</em> strains carrying <em>bla</em>_NDM-5, predominantly of clinical origin, with the highest prevalence in China and the United States. Clonal dissemination was observed both within hospitals and between healthcare facilities in these two countries.</div></div><div><h3>Conclusions</h3><div><em>bla</em>_NDM-5-positive <em>ST</em>171 <em>E. hormaechei</em> strains have emerged globally, with significant prevalence in China and the United States. Our study underscores the clonal spread of these strains within hospitals and across healthcare facilities. Continuous surveillance of these strains is crucial for the effective prevention and control of antimicrobial resistance.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 59-63"},"PeriodicalIF":3.7,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}