Journal of global antimicrobial resistance最新文献

{"title":"Pseudomonas aeruginosa in wound infections: Genomic characterization and emergence of hypervirulent ST1965/ST3418 strains co-harbouring exoU and exoS","authors":"Xin Hong ,&nbsp;Zexuan Li ,&nbsp;Wenying Xia ,&nbsp;Zhongming Tan ,&nbsp;Yulin Hu ,&nbsp;Litao Zhang ,&nbsp;Genyan Liu","doi":"10.1016/j.jgar.2025.05.007","DOIUrl":"10.1016/j.jgar.2025.05.007","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the phenotype and genotype characteristics of <em>Pseudomonas aeruginosa</em> isolates from wound infections.</div></div><div><h3>Methods</h3><div>Seventy-six <em>P. aeruginosa</em> strains isolated from wound infections in a university hospital were analysed. Antimicrobial susceptibility testing, biofilm formation assays, and whole-genome sequencing were performed on all strains. The virulence of potential hypervirulent strains was assessed using a <em>Galleria mellonella</em> infection model.</div></div><div><h3>Results</h3><div>Among the 76 tested strains, 49 (64.5%) were susceptible to all tested antibiotics. The β-lactamase-encoding gene positivity rate was 57.9%, while the <em>OprD</em> gene mutation rate was 1.3%. All isolates were classified into 56 distinct multilocus sequence types. Serotype distribution revealed O11 (22.37%, 17/76), O16 (19.74%, 15/76), and O1 (18.42%, 14/76) as the most prevalent. The <em>exoU</em> gene was predominantly associated with serotype O11. Over 80% of strains harboured biofilm-related virulence genes, and all exhibited strong biofilm-forming capacity. Six <em>exoU+/exoS+</em> strains (serotype O4) were identified, with ST1965 and ST3418 demonstrating potential hypervirulence in the infection model.</div></div><div><h3>Conclusions</h3><div><em>P. aeruginosa</em> isolates from wound infections displayed sporadic genomic profiles, high antibiotic susceptibility, and robust biofilm formation. The emergence of <em>exoU+/exoS+</em> hypervirulent clones (ST1965 and ST3418), characterized by enhanced virulence and biofilm production, highlights their potential to cause treatment-refractory infections and severe clinical outcomes. Continuous surveillance and tailored therapeutic approaches are imperative for managing infections caused by these clones.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 220-228"},"PeriodicalIF":3.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights into virulence, biofilm formation, and antimicrobial resistance of multidrug-resistant Helicobacter pylori strains of novel sequence types isolated from Vietnamese patients with gastric diseases","authors":"Thanh Thuyet Bui ,&nbsp;Thi Thanh Tam Tran ,&nbsp;Thai Son Nguyen ,&nbsp;Thi Thu Hang Le ,&nbsp;Cam Linh Nguyen ,&nbsp;Hoang Nam Pham ,&nbsp;Anne-Laure Bañuls ,&nbsp;Huu Song Le ,&nbsp;Huu Phuong Anh Le ,&nbsp;Thi Tho Bui ,&nbsp;Tien Sy Bui ,&nbsp;Quoc Hoan Phan ,&nbsp;Thi Huyen Trang Tran ,&nbsp;Quang Huy Nguyen","doi":"10.1016/j.jgar.2025.05.001","DOIUrl":"10.1016/j.jgar.2025.05.001","url":null,"abstract":"<div><div><em>Helicobacter pylori</em> (<em>H. pylori</em>) is a clinically important pathogen associated with gastric diseases. Here, we characterized the genome of multidrug-resistant <em>H. pylori</em> strains of novel sequence types, which were recovered from Vietnamese patients with gastritis or a stomach ulcer. Phenotypic-antibiotic susceptibility testing was performed against amoxicillin, clarithromycin, metronidazol, tetracycline, and levofloxacin using an E-test. The whole genome sequence of three <em>H. pylori</em> strains was de novo assembled, followed by in silico analysis of multilocus sequence typing (MLST), core-genome based phylogeny, genetic determinants associated with virulence, biofilm formation, and antibiotic-resistance. The genome sequence of <em>H. pylori</em> strains exhibited a high similarity with the average nucleotide identity (ANI) values of 98.5% to 99.2%, carried 5 to 7 pathogenicity islands, and 3 to 6 mobilomes. The MLST profile of strains revealed novel sequence types ST4511, ST4512, and ST4513. Core-genome based phylogeny exhibited that the three <em>H. pylori</em> strains belong to the Asian genotype. These strains possessed 128 to 131 virulence genes, including toxin-encoding genes <em>cag</em>A and <em>vac</em>A. Double amoxicillin-resistant mutations on <em>pbp</em>1 and <em>pbp</em>2, or a mutation on <em>pbp</em>3, triple clarithromycin-resistant mutations on 23S rRNA gene and a levofloxacin-resistant mutation on <em>gyr</em>A were detected in antibiotic-resistant strains. Mutations on <em>rdx</em>A were detected in both metronidazole-resistant and -sensitive strains, whereas <em>frx</em>A mutations were detected in only one metronidazole-sensitive strain. Finally, a rifamycin-resistant mutation in <em>rpo</em>B was also detected. This study provides insights into the genome of multidrug-resistant <em>H. pylori</em> strains of a novel sequence type circulating in Vietnam for future investigations of its pathobiology and spread through human populations.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 237-241"},"PeriodicalIF":3.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low GAS carriage in school-aged children in western China during the national atypical scarlet fever resurgence: Insights from two cross-sectional studies","authors":"Mengyang Guo ,&nbsp;Xiangping Hou ,&nbsp;Wei Shi ,&nbsp;Qian Huang ,&nbsp;Wei Gao ,&nbsp;Limin Dong ,&nbsp;Yun Lai ,&nbsp;Siyu Chen ,&nbsp;Jianghong Deng ,&nbsp;Kaihu Yao","doi":"10.1016/j.jgar.2025.04.012","DOIUrl":"10.1016/j.jgar.2025.04.012","url":null,"abstract":"<div><h3>Introduction</h3><div>This study examined group A streptococcus(GAS) carriage, <em>emm</em> types, and antibiotic susceptibility in children (6–13 years) in Aral, China, during the post-COVID-19 scarlet fever resurgence, providing regional insights.</div></div><div><h3>Methods</h3><div>The prevalence of GAS carriage was assessed in 1,835 children aged 6–13 years across two surveys at an Aral school in China during the post-COVID-19 resurgence of scarlet fever. GAS isolates were analyzed for <em>emm</em> types, M1_UK lineage, and antimicrobial susceptibility using culture, PCR, sequencing, and automated methods.</div></div><div><h3>Results</h3><div>The first survey (885 children) showed a 1.9% isolation rate, highest in 9-year-olds (4.8%) and slightly higher in boys (2.3% vs. 1.5%, <em>P</em> &gt; 0.05). The second survey (950 children) reported a 3.1% rate, peaking at 10 years (6.7%) and also higher in boys (3.5% vs. 2.6%, <em>P</em> &gt; 0.05). Colonization rates were similar overall (<em>P</em> &gt; 0.05), but increased significantly in children aged ≥10 years (1.1% to 3.3%, <em>P</em> = 0.038). No children tested positive for GAS in both sampling rounds, which meant that the two surveys identified distinct host populations colonized by the bacteria. <em>Emm12</em> prevalence decreased from 76.5% to 55.2% (<em>P</em> &gt; 0.05), while <em>emm1</em> increased from 11.8% to 31.0% (<em>P</em> &gt; 0.05), with no M1_UK lineage detected. All isolates were sensitive to penicillin, linezolid, vancomycin, and levofloxacin. Among 33 co-resistant isolates, <em>emm12</em> accounted for 84.8% and <em>emm1</em> for 15.2%.</div></div><div><h3>Conclusion</h3><div>Despite low GAS carriage rates, variations in age distribution and <em>emm</em> types suggest increased bacterial activity, warranting ongoing monitoring for GAS-related diseases.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 183-187"},"PeriodicalIF":3.7,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-world performance in therapeutic target attainment of various recommended polymyxin B dose regimens: Secondary data analysis of a prospective multicenter cohort","authors":"Jieqiong Liu ,&nbsp;Yuhua Zhao ,&nbsp;Jianping Zhu ,&nbsp;Gang Liang ,&nbsp;Yi Yang ,&nbsp;Lingyan Yu ,&nbsp;Zhenwei Yu","doi":"10.1016/j.jgar.2025.05.003","DOIUrl":"10.1016/j.jgar.2025.05.003","url":null,"abstract":"<div><h3>Background</h3><div>Various population pharmacokinetic studies have suggested controversial optimal dosing regimens for polymyxin B in recent years. The objective of this study was to examine the real-world performance of various dosing regimens in therapeutic target attainment.</div></div><div><h3>Methods</h3><div>This is a secondary analysis of a large prospective multicenter cohort (ChiCTR2200056667). Patients were retrospectively included from the cohort, and patient demographic characteristics, polymyxin B dosing regimens and corresponding 24-hour areas under the curve (AUCs) were collected. Patients were categorized into various groups according to the dosage regimens, and the corresponding AUC target attainment was analyzed. The target AUC ratio was defined as 50–100 mg/L·h.</div></div><div><h3>Results</h3><div>A total of 304 AUC data from 247 patients were included in this study. Only 55.3% of the AUCs were in the range of 50–100 mg/L·h. Differences in subgroups stratified by fixed-dosing regimens, weight-based regimens, and renal function-based dosing regimens. Moreover, the differences among the highest target dose strategies (fixed dose of 50 mg/12 h, weight-adjusted dose of 1–1.25 mg/kg/12 h, and CRRT unadjusted dose) were also insignificant.</div></div><div><h3>Conclusion</h3><div>No polymyxin B dosing strategy is superior in terms of target attainment, which highlights the importance of TDM in the clinical application of polymyxin B.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 229-232"},"PeriodicalIF":3.7,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Y58D mutation in rpsJ gene is correlated with tigecycline and eravacycline combined resistance in ST80 vancomycin-resistant Enterococcus faecium isolates","authors":"Felice Valzano ,&nbsp;Elisa Beccia ,&nbsp;Gianfranco La Bella ,&nbsp;Marianna Marangi ,&nbsp;Fabio Arena","doi":"10.1016/j.jgar.2025.04.028","DOIUrl":"10.1016/j.jgar.2025.04.028","url":null,"abstract":"<div><h3>Objectives</h3><div>To describe the phenotypic and genetic features of vancomycin-resistant Enterococci (VRE) isolates exhibiting resistance to tetracycline (TET), tigecycline (TGC), and eravacycline (ERV).</div></div><div><h3>Methods</h3><div>Between December 2023 and September 2024, 32 VRE were collected from clinical samples at the Policlinico Foggia, Italy. Bacterial identification was carried out by matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis. Antimicrobial susceptibility testing for vancomycin, TET, TGC, and ERV was performed by the reference broth microdilution method. Whole-genome sequencing was carried out to explore the content of tetracycline resistance determinants, and the phylogenetic relationship between isolates (in silico multilocus sequence typing [MLST] and single-nucleotide polymorphisms [SNPs] analysis).</div></div><div><h3>Results</h3><div>Among the studied isolates, 30 and 2 were vancomycin-resistant <em>Enterococcus faecium</em> (VREfm) and vancomycin-resistant <em>Enterococcus faecalis</em> (VREfs), respectively<em>.</em> Overall, eight isolates (six VREfm and two VREfs) were resistant to TET (8/32, 25%), with four (all VREfm) being resistant to TGC and ERV (4/32, 12.5%), also. The two TET-resistant VREfs strains belonged to sequence type (ST)6 and carried the <em>tet</em>(M) gene only. All the TET-resistant VREfm were ST80 and carried the <em>tet</em>(L) and <em>tet</em>(M) genes. Among these, the four TGC-resistant and ERV-resistant strains showed an uncommon Y58D substitution in the ribosomal S10 protein (<em>rpsJ</em> gene). SNPs analysis revealed that Y58D-carrying strains formed a separate cluster, within ST80 VREfm.</div></div><div><h3>Conclusions</h3><div>In this study, we correlated the presence of the Y58D mutation in the <em>rpsJ</em> gene with resistance to TGC and ERV in a cluster of VREfm ST80 clinical strains. The Y58D mutation was invariably found in co-presence with the <em>tet</em>(L) and <em>tet</em>(M) genes.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 242-247"},"PeriodicalIF":3.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colistin-resistant Klebsiella pneumoniae species complex: The scenario in Mexico","authors":"Jonathan Rodríguez-Santiago ,&nbsp;Alejandro Alvarado-Delgado ,&nbsp;Nadia Rodríguez-Medina ,&nbsp;Elvira Garza-González ,&nbsp;Juan Tellez-Sosa ,&nbsp;Luis Duarte-Zambrano ,&nbsp;Neli Nava-Domínguez ,&nbsp;Christian Sohlenkamp ,&nbsp;Miguel A. Vences-Guzmán ,&nbsp;Luis Esaú López-Jácome ,&nbsp;Rayo Morfin-Otero ,&nbsp;Eduardo Rodriguez-Noriega ,&nbsp;Rigoberto Hernández-Castro ,&nbsp;Christian Mireles-Dávalos ,&nbsp;Eduardo Becerril-Vargas ,&nbsp;Juan Pablo Mena-Ramírez ,&nbsp;Edgar Cruz-García ,&nbsp;Ulises Garza-Ramos","doi":"10.1016/j.jgar.2025.04.024","DOIUrl":"10.1016/j.jgar.2025.04.024","url":null,"abstract":"<div><h3>Objective</h3><div>Characterize the colistin-resistance mechanisms, determine the molecular epidemiology, and genomic traits of the colistin-resistant <em>Klebsiella pneumoniae</em> species complex (ColR-KpSC) clinical isolates in Mexico.</div></div><div><h3>Methods</h3><div>In this study, 1539 KpSC isolates were collected in Mexico from 2016 to 2021. We conducted a comprehensive analysis that included microbiological, genetic, molecular, and genomic approaches.</div></div><div><h3>Results</h3><div>A total of 50 isolates (3.25%) were colistin-resistant; of which 49 (98%) corresponded to <em>K. pneumoniae</em> and 1 (2%) to <em>Klebsiella quasipneumoniae</em>. Whole genome sequencing of these resistant isolates revealed intra- and inter-hospital dissemination, and the <em>mgrB</em> inactivation was the main resistance mechanism. Some KpSC isolates carried plasmid-borne <em>mcr-1</em> gene found in <em>Escherichia coli</em> from piglets in Mexico. The colistin-resistant isolates presented a high prevalence of extended-spectrum β-lactamases- and NDM-1 genes, and one hypervirulent strain also produced extended-spectrum β-lactamases CTX-M-15.</div></div><div><h3>Conclusions</h3><div>This study provides a comprehensive snapshot of the epidemiology of ColR-KpSC in Mexico, highlighting a high prevalence of NDM-1 carbapenemase among ColR-KpSC isolates; this is in line with previous reports identifying NDM-1 as the most prevalent carbapenemase in KpSC. This problem is particularly concerning in Mexico because of the lack of therapeutic options.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 203-212"},"PeriodicalIF":3.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of microbroth dilution and e-test methods for detecting Fosfomycin susceptibility in pseudomonas aeruginosa","authors":"Gongqing Song,&nbsp;Xiuzhen Xu","doi":"10.1016/j.jgar.2025.04.014","DOIUrl":"10.1016/j.jgar.2025.04.014","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Pseudomonas aeruginosa</em>’s susceptibility to fosfomycin was assessed using microbroth dilution (MBD) and E-test methods. The most reliable method was determined by comparing the results with those of the reference agar dilution (AD) method.</div></div><div><h3>Methods</h3><div>A total of 62 <em>P. aeruginosa</em> isolates from 62 patients, comprising 29 carbapenem-resistant (CRPA) and 33 non-carbapenem-resistant (non-CRPA) strains, were collected. Drug susceptibility was assessed using the AD, MBD, and E-test methods. According to the recommendations of CLSI and EUCAST, using AD as a reference, the results were interpreted using 64 µg/mL as the breakpoint. The evaluation metrics included categorical agreement (CA), major error (ME; false-resistant), very major error (VME; false-susceptible), and Cohen’s kappa values.</div></div><div><h3>Results</h3><div>The results indicated that the susceptibility of all three methods exceeded 85%, with both MIC50 and MIC90 values being ≥ 64 µg/mL, and no significant difference was observed among them. For different <em>P. aeruginosa</em> types, the E-test demonstrated substantial variability compared with the other two methods. Finally, the MBD and E-test were compared with AD, with VME and Cohen’s kappa values of 1.61%/6.5%, and 71.37%/28.4%, respectively.</div></div><div><h3>Conclusions</h3><div>Fosfomycin demonstrated significant activity against <em>P. aeruginosa.</em> MBD is more suitable than the E-test for assessing the susceptibility of fosfomycin against <em>P. aeruginosa</em>. Based on our results, we firmly believe that the MBD method can serve as an alternative to the AD method for detecting CRPA.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 130-133"},"PeriodicalIF":3.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimodal radiomics integrating deep learning and clinical features for diagnosing multidrug-resistant tuberculosis in HIV/AIDS patients","authors":"Chang Song ,&nbsp;Aichun Huang ,&nbsp;Chunyan Zhao ,&nbsp;Lemin Wen ,&nbsp;Shulin Song ,&nbsp;Yanrong Lin ,&nbsp;Chaoyan Xu ,&nbsp;Hangbiao Qiang ,&nbsp;Qingdong Zhu","doi":"10.1016/j.jgar.2025.04.013","DOIUrl":"10.1016/j.jgar.2025.04.013","url":null,"abstract":"<div><h3>Background</h3><div>This study aimed to develop and validate a predictive model based on multimodal data, including clinical features, radiomics features, and deep learning features, to distinguish multidrug-resistant tuberculosis (MDR-TB) in HIV/AIDS patients, thereby improving diagnostic accuracy.</div></div><div><h3>Methods</h3><div>A retrospective cohort of HIV/AIDS patients with drug-sensitive tuberculosis (<em>n</em> = 164) and MDR-TB (<em>n</em> = 63) admitted to the Fourth People's Hospital of Nanning between January 2016 and July 2024 was included. The dataset was randomly divided into training and validation sets at a 7:3 ratio. A multimodal model was constructed by integrating a clinical model, a radiomics model, and a 2.5D multi-instance learning (MIL) approach.</div></div><div><h3>Results</h3><div>Key predictors—platelet count and C-reactive protein—were identified through univariate and multivariate logistic regression analysis. The integrated model achieved the highest performance in both the training and validation set (AUC=0.943 and 0.899, respectively), significantly outperforming individual models. Grad-CAM effectively localized key image regions influencing decision-making, while a nomogram quantified the contribution weights of each predictor, enhancing model transparency. The Hosmer-Lemeshow (HL) test confirmed good model calibration, and the decision curve analysis (DCA) curve demonstrated the optimal clinical net benefit of the integrated model.</div></div><div><h3>Conclusion</h3><div>The multimodal integrated model developed in this study significantly improved the diagnostic efficacy of MDR-TB in HIV/AIDS patients by combining clinical, radiomics, and deep learning features, providing a reliable tool for individualized precision diagnosis and treatment.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 134-142"},"PeriodicalIF":3.7,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbapenem-resistant hypervirulent P. aeruginosa coexpressing exoS/exoU in Brazil","authors":"Sergio M. Morgado ,&nbsp;Nathália M.S. Bighi ,&nbsp;Fernanda S. Freitas ,&nbsp;Caio Rodrigues ,&nbsp;Érica L. Fonseca ,&nbsp;Ana Carolina Vicente","doi":"10.1016/j.jgar.2025.04.025","DOIUrl":"10.1016/j.jgar.2025.04.025","url":null,"abstract":"<div><h3>Background</h3><div><em>Pseudomonas aeruginosa</em> is a ubiquitous organism with high clinical impact due to its intrinsic resistance to many antimicrobial drugs and ability to acquire antibiotic resistance and virulence genes. The type III secretion system (T3SS) is critical in the pathogenesis of this species, where ExoS and ExoU are the main toxins. Strains cocarrying both toxins are considered hypervirulent. However, there is a gap in revealing the coexpression of <em>exo</em>U/<em>exo</em>S. This study characterizes a carbapenem-resistant <em>exo</em>U+/<em>exo</em>S+ <em>P. aeruginosa</em> in Brazil through its expression and genetic context.</div></div><div><h3>Methods</h3><div>Thirty-three <em>P. aeruginosa</em> from Brazil were subjected to antibiotic susceptibility testing and sequencing. The genomes were characterized considering their resistome and virulome, and their phylogeny was established. The expression of <em>exo</em>U/<em>exo</em>S was evaluated by qPCR and the <em>exo</em>U genomic region was analyzed.</div></div><div><h3>Results</h3><div>A carbapenem-resistant strain presented the <em>exo</em>U+/<em>exo</em>S+ genotype, belonging to serotype O4 and ST4996. Although it did not carry carbapenemases, it presented mutations in the porin OprD. The qPCR assay showed that <em>exo</em>U and <em>exo</em>S are coexpressed at higher levels than control strains, although they share the same promoter and relevant ExoU sites with <em>exo</em>U+/<em>exo</em>S- genomes. Three other Brazilian genomes with this trait were identified, also presenting the <em>exo</em>U gene in the context of the PAPI-2 island.</div></div><div><h3>Conclusion</h3><div><em>P. aeruginosa</em> carrying <em>exo</em>S/<em>exo</em>U in Brazil is rare, although they may occur in the clinic associated with carbapenem resistance. Furthermore, although belonging to a different ST, the Brazilian strain PA27N presented <em>exo</em>S/<em>exo</em>U expression levels similar to the hypervirulent <em>P. aeruginosa</em> strains occurring in China.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 233-236"},"PeriodicalIF":3.7,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ISAC News and UpdatesISAC News and Updates","authors":"","doi":"10.1016/j.jgar.2025.04.003","DOIUrl":"10.1016/j.jgar.2025.04.003","url":null,"abstract":"","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"42 ","pages":"Pages 262-263"},"PeriodicalIF":3.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}