Journal of global antimicrobial resistance最新文献

{"title":"Molecular characterization of ST15 carbapenem-resistant Klebsiella pneumoniae isolated in a single patient","authors":"Yongjin Hu , Rong Tang , Shanshan Jin , Jiahao Guan , Xiaoxiao Meng , Zengpeijie Dan , Ruilan Wang , Hong-Yu Ou , Jian Lu","doi":"10.1016/j.jgar.2024.11.003","DOIUrl":"10.1016/j.jgar.2024.11.003","url":null,"abstract":"<div><h3>Background</h3><div>The carbapenem-resistant <em>Klebsiella pneumoniae</em> (CRKP) poses a serious threat to antibiotic applicability and public health. During treatment, <em>K. pneumoniae</em> (KP) frequently exhibits shifts in drug-resistant phenotypes, complicating clinical treatment as it transitions from sensitivity to resistance. In this study, we analysed the clinical and molecular characteristics of drug resistance changes in KP strains isolated from a single patient, and the potential mechanisms underlying these resistance changes.</div></div><div><h3>Methods</h3><div>Antimicrobial susceptibility test and string test were conducted to evaluate the resistant and virulent characterization of the strains. Pulsed-field gel electrophoresis (PFGE) was used to investigate the homology relationship between the strains. The whole genome sequencing and phylogenetic analysis of 9 representative isolates was also performed. The transfer ability of the drug-resistant plasmid was studied by plasmid conjugation experiment. The transconjugants were verified by polymerase chain reaction amplification of specific genes, antimicrobial susceptibility test and PFGE.</div></div><div><h3>Results</h3><div>Our results revealed that 9 KP strains, isolated from the same patient, exhibited ‘resistance-sensitivity-resistance-sensitivity’ alternately to carbapenems. The differences in DNA fingerprint bands among the nine KP isolates were ≤3, which can be classified as the same PFGE type. Phylogenetic analysis showed that these 9 strains constituted a distinct branch within the phylogenetic tree. All nine KP strains belonged to the ST15-KL19 clone. Six of the strains were classified as CRKP, all of which carried 11 drug resistance genes: <em>oqxB, oqxA, fosA6, aac(3)-lld, bla</em><sub>SHV-28</sub>, <em>bla</em><sub>KPC-2</sub>, <em>bla</em><sub>OXA-1</sub>, <em>mph(A), tet(A), catB3</em> and <em>aac(6′)-lb-cr</em>, mediating drug resistance to quinolones, fosfomycin, aminoglycosides, β-lactam, carbapenems, macrolides and chloramphenicol, belonging to multi-drug resistant bacteria. The carbapenem-resistant plasmid p2-KP3762–1 was found to transfer within species, from CRKP to hypervirulent KPRJF293HA, carbapenem-sensitive KP KP3657 and <em>Escherichia coli</em> C600 at a frequency of (1.19 ± 1.58) ×10<sup>−6</sup>, (1.09 ± 1.38) ×10<sup>−7</sup> and (10.9 ± 9.53) ×10<sup>−6</sup> respectively, resulting in the dissemination of carbapenem resistance genes.</div></div><div><h3>Conclusions</h3><div>In this study, KP strains isolated from a single patient exhibited an alternating phenotype of resistant-sensitive-resistant-sensitive to carbapenems. The 9 KP isolates share a high degree of genetic similarity. The plasmid p2-KP3762–1, harbouring the carbapenem resistance gene <em>bla</em><sub>KPC-2</sub>, may undergo inter-strain and inter-clone transfer via conjugation in the patient during treatment. Furthermore, our findings suggest that the pathogens in this pati","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 72-80"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ISAC Information Page","authors":"","doi":"10.1016/S2213-7165(25)00027-X","DOIUrl":"10.1016/S2213-7165(25)00027-X","url":null,"abstract":"","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Page i"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143269903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive analysis of the epidemiological and genomic characteristics of global Serratia Marcescens","authors":"Wenqin Chen ,&nbsp;Zheqian Li ,&nbsp;Jie Zheng ,&nbsp;Danwei Wang ,&nbsp;Shuo Gao ,&nbsp;Wanqing Zhou ,&nbsp;Han Shen ,&nbsp;Yan Zhang ,&nbsp;Xiaoli Cao","doi":"10.1016/j.jgar.2024.11.013","DOIUrl":"10.1016/j.jgar.2024.11.013","url":null,"abstract":"<div><h3>Background</h3><div><em>Serratia marcescens</em> outbreaks present significant challenges in clinical treatment, necessitating a deeper understanding of its epidemiological and genomic traits.</div></div><div><h3>Objective</h3><div>To analyse the epidemiological and genomic characteristics of <em>S. marcescen</em>s at a global scale.</div></div><div><h3>Methods</h3><div>High-quality genomes of <em>S. marcescens</em> were retrieved from NCBI and annotated using Prodigal. Antibiotic resistance genes (ARGs) were identified via Blastn, sequence types (STs) were determined with a proprietary tool, and phylogenetic analysis was conducted to explore evolutionary relationships.</div></div><div><h3>Results</h3><div>The study analysed genomes from 33 countries, with major contributions from the USA (27.8%), UK (15.3%), Italy (14.7%), and Japan (10.7%). Human clinical samples accounted for 73.5% of the isolates, primarily from blood (44.8%) and sputum (19.3%). Eleven ARGs were identified, with <em>sde</em> being the most prevalent. Carbapenemase genes included <em>bla</em>SME, <em>bla</em>KPC, and <em>bla</em>NDM-1, though co-occurrence in individual strains was absent. Novel ARGs, including <em>armA, rmtC</em>, and <em>fosA7.2</em>, were reported. Among 855 genomes with identified STs, ST366, ST367, ST365, and ST423 were most common. Phylogenetic analysis highlighted significant genetic diversity and distinct evolutionary lineages.</div></div><div><h3>Conclusion</h3><div>Temporal analysis showed a genome peak in 2019, underscoring the global prevalence and adaptability of <em>S. marcescens</em>. The distribution of ARGs across diverse STs emphasizes horizontal gene transfer as a key driver of resistance. Judicious antibiotic use is essential to mitigate further resistance.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 81-89"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surveillance and characteristics of vancomycin-resistant Enterococcus isolates in a Chinese tertiary hospital in Shenzhen, 2018 to 2024","authors":"Hongwei Shen ,&nbsp;Qiaomin Zhang ,&nbsp;Shaobo Li ,&nbsp;Tingting Huang ,&nbsp;Wen Ma ,&nbsp;Daming Wang ,&nbsp;Peng Li","doi":"10.1016/j.jgar.2024.11.002","DOIUrl":"10.1016/j.jgar.2024.11.002","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the prevalence and characteristics of vancomycin-resistant <em>Enterococcus</em> (VRE) isolates in a Chinese tertiary hospital in Shenzhen.</div></div><div><h3>Methods</h3><div>A hospital-based retrospective epidemiological survey of <em>Enterococcus</em> was conducted over a 6.5-y period, from January 2018 to June 2024. The VRE isolates were identified and subjected to screening for the six <em>van</em> genes and multi-locus sequence typing (MLST) genotyping. The clinical characteristics, antimicrobial susceptibility profiles and molecular features were subjected to analysis.</div></div><div><h3>Results</h3><div>A total of 34 non-duplicate VRE isolates were identified, comprising 32 vancomycin-resistant <em>Enterococcus faecium</em> (VREfm) and 2 vancomycin-resistant <em>Enterococcus faecalis</em> (VREfa) strains. Since its initial isolation in 2022, there has been an observable increase in the detection rate of VRE. The detection rate of VRE between 2022 and 2024 (until June) was 0.3%, 4.8%, and 8.6%, respectively. The majority of the VRE strains were isolated from urine (25/34, 73.5%), and the highest detection rate (9.1%) of VRE infections was observed in the patients aged ≥75 y. In excess of 90% of VRE isolates exhibited resistance to ciprofloxacin (97.1%) and levofloxacin (97.1%), followed by ampicillin (94.1%) and penicillin (94.1%). The non-susceptible rate was observed to be low for linezolid (2.9%) and tigecycline (5.9%). Of the 29 VREfm isolates preserved and tested, 28 were found to harbour the <em>vanA</em> gene. A total of six STs were identified among the 29 VREfm isolates, with ST80 (16/29, 55.2%) being the predominant. The ST80 remained the most prevalent clone until the introduction of ST78 in May 2023, at which point these two clones became the most prevalent.</div></div><div><h3>Conclusion</h3><div>There has been an observable increase in the prevalence of VRE in our hospital since 2022. Furthermore, an ongoing outbreak of ST80 and ST78 VREfm with <em>vanA</em>-harboring plasmid was identified. It is imperative that continuous surveillance be conducted in order to inform public health interventions.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 29-33"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and antimicrobial resistance of three clones (ST1223, ST2198, ST2250) of Staphylococcus argenteus clinical isolates in northern Japan","authors":"Meiji Soe Aung ,&nbsp;Noriko Urushibara ,&nbsp;Mitsuyo Kawaguchiya ,&nbsp;Nobuhide Ohashi ,&nbsp;Mina Hirose ,&nbsp;Kenji Kudo ,&nbsp;Masahiko Ito ,&nbsp;Nobumichi Kobayashi","doi":"10.1016/j.jgar.2024.11.004","DOIUrl":"10.1016/j.jgar.2024.11.004","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Staphylococcus argenteus</em>, a newly described species of coagulase-positive <em>Staphylococcus</em>, was first identified in clinical isolates in northern Japan in 2019. The aim of the present study was to clarify the trends in the epidemiological features and antimicrobial resistance traits of <em>S. argenteus</em> in the recent years.</div></div><div><h3>Methods</h3><div><em>S. argenteus</em> isolates from various clinical specimens were screened using MALDI-TOF and genotyped using the multilocus sequence typing scheme. Antimicrobial susceptibility was determined using broth microdilution test and resistance genes were detected by multiplex/uniplex PCR. Nucleotide sequences of haemolysin genes (<em>hla, hlb, hld</em>, and <em>hlg</em>) were determined and analysed in some isolates.</div></div><div><h3>Results</h3><div>From August 2020 to December 2023, 210 isolates were identified as <em>S. argenteus</em>. The ratio of <em>S. argenteus</em> to <em>Staphylococcus aureus</em> clinical isolates was 0.0045, showing a slightly decreasing trend compared to what was previously reported (2019.8–2020.7). Three genotypes, ST1223-<em>coa</em>-XV, ST2198-<em>coa</em>-XIV, and ST2250-<em>coa</em>-XId were identified, with ST2250 being dominant (49%) and exhibiting an increasing trend. <em>S. argenteus</em> isolates were almost susceptible to antimicrobials examined, while ST2198 isolates showed higher resistance rates to ampicillin, macrolides, and aminoglycosides than other clones, harbouring <em>blaZ, msrA</em>, and <em>aac(6′)-Ie-aph(2″)-Ia</em>. Only eight isolates (4-ST2250, 3-ST1223, and 1-ST2198) did not show haemolysis on blood agar plates. These isolates had intact haemolysin genes and promoter regions; however, a novel deletion mutation in <em>hlg</em>-component A was detected in some haemolytic and non-haemolytic isolates.</div></div><div><h3>Conclusions</h3><div>The present study revealed the epidemiological trend of <em>S. argenteus</em> clones in northern Japan, along with the occurrence of non-haemolytic isolates with intact haemolysin genes.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 1-7"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing trend of antimicrobial resistance among methicillin-resistant Staphylococcus aureus strains in Southwest Finland, 2007–2016: An analysis of shifting strain dynamics and emerging risk factors","authors":"Jaakko Silvola ,&nbsp;Kirsi Gröndahl-Yli-Hannuksela ,&nbsp;Tiina Hirvioja ,&nbsp;Kaisu Rantakokko-Jalava ,&nbsp;Mari Kanerva ,&nbsp;Kari Auranen ,&nbsp;Harri Marttila ,&nbsp;Jenna Junnila ,&nbsp;Jaana Vuopio","doi":"10.1016/j.jgar.2024.11.015","DOIUrl":"10.1016/j.jgar.2024.11.015","url":null,"abstract":"<div><h3>Objective</h3><div>Substantial rise in the annual incidence of methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) was reported in Southwest Finland (12.4–24.9/100,000 people) between 2007 and 2016. To understand the implications of these changes to the management of MRSA, we sought to analyse the antimicrobial resistance (AMR) trends of MRSA in relation with patient characteristics.</div></div><div><h3>Methods</h3><div>Antimicrobial susceptibility was determined for 10 clinically relevant antimicrobials. Strains with resistance to ≥2 antimicrobials were defined multi-resistant. The isolates were spa-typed and clustered. AMR trends and risk factors were identified by associating resistant phenotypes with patient demographics.</div></div><div><h3>Results</h3><div>A total of 983 new MRSA cases were identified between 2007 and 2016. After 2011, significant increasing trends were observed in the proportion of isolates resistant to clindamycin (13.9%–31.5%, <em>P</em> &lt; 0.001), erythromycin (19.4%–35.4%, <em>P</em> &lt; 0.001) and tetracycline (16.7%–32%, <em>P</em> &lt; 0.001). The proportion of multi-resistant isolates more than doubled from 14.8% to 39.2%. The increasing AMR trend was reflected in the increase of new strain types and the decrease of previously dominant, non-multi-resistant strains. Patient risk factors associated with (<em>P</em> <em>&lt;</em> 0.001) the acquisition of multi-resistant strains included community acquisition, livestock contact, hospital care abroad and immigrant status.</div></div><div><h3>Conclusions</h3><div>Notable increasing AMR trends among MRSA isolates were observed in Southwest Finland, 2007–2016. The shift in patient demographics to younger age groups and community acquisition contributed to the increase in multi-resistant strains. Immigration, contact with hospital environment abroad and contact with livestock were identified as essential risk factors of multi-resistance. The increased level of co-resistance has persisted after 2016.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 47-52"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas aeruginosa ST1971 clinical strain carrying the blaNDM-1 gene on ICETn43716385 in Greece","authors":"Christos-Georgios Gkountinoudis,&nbsp;Efthymia Petinaki","doi":"10.1016/j.jgar.2024.12.003","DOIUrl":"10.1016/j.jgar.2024.12.003","url":null,"abstract":"","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 98-99"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Draft genome sequence of a co-harbouring blaNDM-5 and mcr-1.1 Escherichia coli phylogroup A isolate associated with patient colonisation in Ireland","authors":"Anna Tumeo ,&nbsp;Francesca McDonagh ,&nbsp;Aneta Kovarova ,&nbsp;Kate Ryan ,&nbsp;Christina Clarke ,&nbsp;Georgios Miliotis","doi":"10.1016/j.jgar.2024.11.018","DOIUrl":"10.1016/j.jgar.2024.11.018","url":null,"abstract":"<div><h3>Objectives</h3><div>While <em>Escherichia coli</em> phylogroup-A is typically associated with commensal strains, some isolates can harbour virulence and exhibit multidrug-resistant (MDR) phenotypes. We report the draft genome of a rare instance of carbapenem, fosfomycin and colistin resistant <em>E. coli</em> phylogroup-A, isolated as part of routine screening of a human patient in a clinical setting in Ireland.</div></div><div><h3>Methods</h3><div><em>E. coli</em> E230738 was identified using MALDI-ToF/MS. Antibiotic susceptibility testing was performed using the Sensititre-EUMDRXXF plate. Whole-genome-sequencing was conducted with NextSeq1000, and genomic analysis identified antibiotic-resistance-genes (ARGs) and virulence-factors (VFs). Phylogenetic analysis was performed using whole-genome-multilocus-sequence-typing (wgMLST).</div></div><div><h3>Results</h3><div><em>E. coli</em> E230738 genome was identified to belong to phylogroup-A/ST10 complex and to harbour 63 ARGs, 17 of which acquired. Resistance to beta-lactams, including carbapenems and cephalosporins was likely due to chromosomally identified <em>bla</em>_NDM-5. Colistin resistance appeared associated with acquired <em>mcr</em>-1.1. Despite lacking fosfomycin-inactivating-enzymes, fosfomycin resistance was observed, possibly due to efflux pumps. Forty-seven chromosomal VFs were identified, involved in adhesion and iron acquisition amongst other properties. Plasmid replicons associated with the spread of MDR genes such as IncHI2/HI2A were detected. wgMLST analysis showed the closest relative being a strain from the UK, exhibiting differences in the sequences of 851 genes.</div></div><div><h3>Conclusion</h3><div>This is a first detected instance of a <em>bla</em>_NDM-5 and <em>mcr</em>-1.1 co-occurring in <em>E. coli</em> in Ireland. The MDR profile of <em>E. coli</em> E230738 highlights the growing public health concern posed by the dissemination of MDR <em>E. coli</em> lineages with limited treatment options and underscores the need for clinical screening coupled with genomic surveillance to better understand evolving MDR patterns in <em>E. coli</em>.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"40 ","pages":"Pages 62-65"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clonal dissemination of methicillin-resistant Staphylococcaceae between Algerian sheep farms","authors":"Chahrazed Belhout ,&nbsp;Javier E. Fernandez ,&nbsp;Patrick Butaye ,&nbsp;Vincent Perreten","doi":"10.1016/j.jgar.2024.12.017","DOIUrl":"10.1016/j.jgar.2024.12.017","url":null,"abstract":"<div><h3>Objectives</h3><div>Sheep farming represents an important economic sector in Algeria, and the potential dissemination of methicillin-resistant <em>Staphylococcaceae</em> (MRS) is a critical veterinary and public health concern. This study aimed to determine the prevalence and types of MRS in ovine in Algeria and characterize them using whole-genome sequencing (WGS) analysis.</div></div><div><h3>Methods</h3><div>Two hundred sheep from 20 different Algerian farms across 3 regions were screened for nasal colonization with MRS. The isolates were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), their minimal inhibitory concentration (MIC) was determined by broth microdilution, and the presence of the <em>mec</em> gene was confirmed with polymerase chain reaction (PCR). The <em>mec</em>-positive isolates were sequenced using Illumina technology to build species specific core genome multilocus sequence typing (cgMLST)- and single nucleotide polymorphisms (SNPs)-based phylogenies and perform an in silico screening for antimicrobial resistance genes.</div></div><div><h3>Results</h3><div>The prevalence of MRS-positive farms was 85% (95% confidence interval [CI] = 69.34%–100%) across the sampled farms. Ten distinct <em>Staphylococcaceae</em> species were identified, with <em>Staphylococcus saprophyticus</em> (<em>S. saprophyticus; n</em> = 29), <em>Mammaliicoccus lentus</em> (<em>M. lentus; n</em> = 24), and <em>Staphylococcus haemolyticus</em> (<em>S. haemolyticus; n</em> = 19) being the predominant species. WGS-based phylogeny and SNP analysis (0 to 126 SNPs) revealed that isolates of these three species were highly related, indicating clonal dissemination within and between farms. MRS exhibited a multi-drug resistance pattern, with detection of resistance genes for β-lactams, tetracyclines, fusidic acid, trimethoprim, aminoglycosides, tiamulin, and macrolides.</div></div><div><h3>Conclusions</h3><div>Specific clonal lineages of methicillin-resistant <em>S. saprophyticus, S. haemolyticus</em>, and <em>M. lentus</em> are widespread in Algerian sheep farms. Enhancing hygiene practices on farms is recommended to prevent further dissemination of these resistant strains to animals and humans. © 2025 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"41 ","pages":"Pages 96-104"},"PeriodicalIF":3.7,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of Acinetobacter phage vAbaIN10 active against carbapenem-resistant Acinetobacter baumannii (CRAB) isolates from healthcare-associated infections in Dakar, Senegal","authors":"Issa Ndiaye ,&nbsp;Laurent Debarbieux ,&nbsp;Ousmane Sow ,&nbsp;Bissoume Sambe Ba ,&nbsp;Moussa Moise Diagne ,&nbsp;Abdoulaye Cissé ,&nbsp;Cheikh Fall ,&nbsp;Yakhya Dieye ,&nbsp;Ndongo Dia ,&nbsp;Guillaume Constantin de Magny ,&nbsp;Abdoulaye Seck","doi":"10.1016/j.jgar.2024.12.024","DOIUrl":"10.1016/j.jgar.2024.12.024","url":null,"abstract":"<div><h3>Background</h3><div>Carbapenem-resistant <em>Acinetobacter baumannii</em> (CRAB) is a critical antimicrobial resistance threat and a WHO-prioritized pathogen. With intrinsic resistance to multiple antibiotics and the emergence of pan-resistant isolates, CRAB infections are challenging to treat, often relying on polymyxins, tigecycline, aminoglycosides, or combinations, though co-resistance is rising globally. Phage therapy is considered as a potential treatment for multidrug-resistant <em>A. baumannii</em>. This study focused on isolating and characterizing phages active against CRAB strains from healthcare-associated infections in Dakar, Senegal.</div></div><div><h3>Methods</h3><div>A lytic phage, <em>Acinetobacter</em> vAbaIN10, was isolated from wastewater collected at the Aristide Le Dantec Hospital in Dakar, Senegal. Isolation, host range, efficiency of plating, temperature and pH stability, lysis kinetics, one-step growth test, sequencing, and genomic analysis were performed.</div></div><div><h3>Results</h3><div>Phage vAbaIN10 belongs to the class <em>Caudoviricetes</em> and the genus <em>Friunavirus</em>. Its genome is 40,279 bp in size. Phage vAbaIN10 is stable across a wide pH range (3–9) and temperature range (25°C–60°C). The phage's lytic activity was evaluated at different multiplicities of infection (MOI): MOI 10, 1, and 10⁻¹. All MOIs significantly reduced the growth of host bacteria. The one-step growth curve showed that vAbaIN10 had a latency period of 25 min and a burst size of approximately 4.78 × 10³ phages per infected bacterial cell. No tRNA, mtRNA, clustered regularly interspaced short palindromic repeat, virulence factors, or antibiotic resistance genes were found in the genome.</div></div><div><h3>Conclusions</h3><div>The biological and genomic characteristics of vAbaIN10 meet the requirements for its potential use in phage therapy.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"41 ","pages":"Pages 151-158"},"PeriodicalIF":3.7,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}