The Y58D mutation in rpsJ gene is correlated with tigecycline and eravacycline combined resistance in ST80 vancomycin-resistant Enterococcus faecium isolates

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES
Felice Valzano , Elisa Beccia , Gianfranco La Bella , Marianna Marangi , Fabio Arena
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Abstract

Objectives

To describe the phenotypic and genetic features of vancomycin-resistant Enterococci (VRE) isolates exhibiting resistance to tetracycline (TET), tigecycline (TGC), and eravacycline (ERV).

Methods

Between December 2023 and September 2024, 32 VRE were collected from clinical samples at the Policlinico Foggia, Italy. Bacterial identification was carried out by matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis. Antimicrobial susceptibility testing for vancomycin, TET, TGC, and ERV was performed by the reference broth microdilution method. Whole-genome sequencing was carried out to explore the content of tetracycline resistance determinants, and the phylogenetic relationship between isolates (in silico multilocus sequence typing [MLST] and single-nucleotide polymorphisms [SNPs] analysis).

Results

Among the studied isolates, 30 and 2 were vancomycin-resistant Enterococcus faecium (VREfm) and vancomycin-resistant Enterococcus faecalis (VREfs), respectively. Overall, eight isolates (six VREfm and two VREfs) were resistant to TET (8/32, 25%), with four (all VREfm) being resistant to TGC and ERV (4/32, 12.5%), also. The two TET-resistant VREfs strains belonged to sequence type (ST)6 and carried the tet(M) gene only. All the TET-resistant VREfm were ST80 and carried the tet(L) and tet(M) genes. Among these, the four TGC-resistant and ERV-resistant strains showed an uncommon Y58D substitution in the ribosomal S10 protein (rpsJ gene). SNPs analysis revealed that Y58D-carrying strains formed a separate cluster, within ST80 VREfm.

Conclusions

In this study, we correlated the presence of the Y58D mutation in the rpsJ gene with resistance to TGC and ERV in a cluster of VREfm ST80 clinical strains. The Y58D mutation was invariably found in co-presence with the tet(L) and tet(M) genes.
rpsJ基因Y58D突变与ST80耐万古霉素屎肠球菌替加环素和依拉瓦环素联合耐药有关。
目的:研究耐万古霉素屎肠球菌(VREfm)对四环素(TET)、替加环素(TGC)和依拉瓦环素(ERV)耐药的表型和遗传特征。方法:2023年12月至2024年9月,在意大利福贾警察局采集临床标本中32株万古霉素耐药肠球菌(VRE)。采用质谱法(MALDI-TOF)进行细菌鉴定。采用对照肉汤微量稀释法对万古霉素(VA)、TET、TGC和ERV进行药敏试验。采用全基因组测序(WGS)研究了四环素耐药决定因子的含量,以及分离株间的系统发育关系(采用硅基MLST和snp分析)。结果:分离株中,粪肠球菌和粪肠球菌分别为32株和32株。总体而言,8株分离株,6株VREfm和2株VREfs对TET耐药(8/ 32,25%),4株(全部VREfm)对TGC和ERV耐药(4/ 32,12.5%)。这2株抗tet的VREfs属ST6株,仅携带tet(M)基因。所有抗tet的VREfm均为ST80,携带tet(L)和tet(M)基因。其中,4株tgc耐药和erv耐药菌株在核糖体S10蛋白(rpsJ基因)中出现罕见的Y58D替代。snp分析显示,携带y58d的菌株在ST80 VREfm内形成了一个单独的簇。结论:在本研究中,我们将rpsJ基因中Y58D突变的存在与VREfm ST80临床菌株对TGC和ERV的耐药性联系起来。Y58D突变总是与tet(L)和tet(M)基因共存。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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