Journal of global antimicrobial resistance最新文献

{"title":"In vitro activity of sulopenem and comparator agents against US Enterobacterales clinical isolates collected during the SENTRY antimicrobial surveillance program in 2023","authors":"Steven I. Aronin ,&nbsp;Michael D. Huband ,&nbsp;Holly Becker ,&nbsp;Kelley A. Fedler ,&nbsp;Michael W. Dunne","doi":"10.1016/j.jgar.2025.06.013","DOIUrl":"10.1016/j.jgar.2025.06.013","url":null,"abstract":"<div><h3>Objective</h3><div>Sulopenem is a thiopenem antibacterial with an oral and parenteral formulation. Sulopenem etzadroxil/probenecid, the oral formulation, was recently approved by the United States Food and Drug Administration for the treatment of women with uncomplicated urinary tract infection (UTI). This study evaluated the in vitro activity of sulopenem and comparator agents against contemporary Enterobacterales clinical isolates predominantly from patients with UTIs.</div></div><div><h3>Methods</h3><div>A contemporary collection of 1086 community- and nosocomial-acquired Enterobacterales isolates was assembled from US medical centres. Isolates were susceptibility tested using the Clinical and Laboratory Standards Institute broth microdilution reference method.</div></div><div><h3>Results</h3><div>Sulopenem demonstrated potent in vitro antimicrobial activity (MIC_50/90, 0.03/0.25 mg/L) against Enterobacterales isolates regardless of infection type, inhibiting 98.0% of isolates at ≤0.5 mg/L. This activity was conserved against resistant phenotypes, including extended-spectrum β-lactamase (ESBL)-phenotype <em>Escherichia coli</em> (MIC_50/90, 0.03/0.06 mg/L) and ESBL-phenotype <em>Klebsiella pneumoniae</em> (MIC_50/90, 0.06/0.12 mg/L). As would be expected, cross-resistance was found with imipenem and meropenem to a lesser extent. Sulopenem maintained activity against ciprofloxacin-, nitrofurantoin-, and trimethoprim/sulfamethoxazole-non-susceptible subsets, including urinary isolates from patients in the community (MIC_50/90, 0.03–0.12/0.12–0.5 mg/L). Sulopenem also maintained activity against community-acquired ESBL-producing Enterobacterales urinary isolates non-susceptible to two or more oral antimicrobial agents commonly used to treat UTIs.</div></div><div><h3>Conclusions</h3><div>The potent in vitro activity of sulopenem against this large collection of contemporary Enterobacterales clinical isolates from multiple infection types supports its use in the treatment of uncomplicated UTI, as well as its further clinical evaluation in the treatment of other common bacterial infections demonstrating resistant phenotypes.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 152-159"},"PeriodicalIF":3.7,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence of mcr-1.1-producing Escherichia coli in clinical settings in Tunisia","authors":"Nadia Jaidane ,&nbsp;Thierry Naas ,&nbsp;Sameh Boughattas ,&nbsp;Laetitia Du Fraysseix ,&nbsp;Lamia Tilouche ,&nbsp;Meriem Souguir ,&nbsp;Pauline François ,&nbsp;Wajdi Chermiti ,&nbsp;Abdelhalim Trabelsi ,&nbsp;Jean-Yves Madec ,&nbsp;Wejdene Mansour ,&nbsp;Marisa Haenni","doi":"10.1016/j.jgar.2025.06.011","DOIUrl":"10.1016/j.jgar.2025.06.011","url":null,"abstract":"<div><h3>Objective</h3><div>Our study aims at characterizing a <em>mcr-1</em>-producing <em>Escherichia coli</em> ST224 clinical isolate collected at the University Hospital of Sahloul-Sousse from a joint infection.</div></div><div><h3>Methods</h3><div>Phenotypic characterization was performed using a Vitek-2 System. NGS was performed by short-read (150 bp paired-end reads) sequencing on a NovaSeq6000 platform, as well as by long-read sequencing using an ONT R10.4 flow cell. Hybrid assembly was achieved using the Flye de novo assembler, and analyses were performed using tools of the Center for Genomic Epidemiology. The <em>mcr-1</em>-carrying plasmid was compared by BLAST to similar plasmids recovered in the NCBI database and visualized by PROKSEE.</div></div><div><h3>Results</h3><div>The collected <em>E. coli</em> belonged to ST224 and was multi-drug resistant, remaining susceptible to carbapenems, amikacin, gentamicin, and sulfamethoxazole only. Resistances to last-generation cephalosporins (mediated by <em>bla</em>_CMY-2) and fluoroquinolones were chromosomally-encoded. The <em>mcr-1.1</em> gene was carried by an Inc12 plasmid similar but not identical to published data, and that lacked the two copies of the IS<em>Apl1</em> element usually bracketing the <em>mcr-1.1</em> and <em>pap2</em> genes.</div></div><div><h3>Conclusions</h3><div>The dissemination of plasmid-encoded <em>mcr-1</em> genes that can spread across species and sectors is a major health issue in countries such as Tunisia, where colistin is essential for the treatment of complicated human infections due to carbapenem-resistant Enterobacterales. Reporting all <em>mcr-1</em>-positive <em>E. coli</em> clinical isolates is thus important to understand the dynamic of spread of these resistant pathogens.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 149-151"},"PeriodicalIF":3.7,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory role of ISEcp1 and spacer sequences in blaCTX-M–mediated cephalosporin resistance in Shigella","authors":"Hui Zhang ,&nbsp;Shengkun He ,&nbsp;Xiaoying Pang ,&nbsp;Xi He ,&nbsp;Xuechun Liu ,&nbsp;Shaofu Qiu ,&nbsp;Chaojie Yang ,&nbsp;Hongbin Song","doi":"10.1016/j.jgar.2025.06.005","DOIUrl":"10.1016/j.jgar.2025.06.005","url":null,"abstract":"<div><h3>Background</h3><div>Extended-spectrum β-lactamases, especially CTX-M enzymes, play a critical role in cephalosporin resistance in several bacterial species. The IS<em>Ecp1</em> insertion sequence, a mobile genetic element, regulates the high expression and horizontal transfer of the <em>bla</em>_CTX-M gene. Diverse types of spacer regions exist, the length of which varies across the different types of <em>bla</em>_CTX-M genes and their upstream IS<em>Ecp1</em> elements. In this study, we aimed to investigate the distribution and role of IS<em>Ecp1</em> and different length spacers in antibiotic-resistant <em>Shigella</em>.</div></div><div><h3>Methods</h3><div>We selected 1393 cephalosporin-resistant <em>Shigella</em> strains isolated from China between 2004 and 2016 to analyse the distribution of IS<em>Ecp1</em> and spacers using polymerase chain reaction and sequencing. To functionally evaluate the role of these elements, we designed different combinations of IS<em>Ecp1</em>, spacer sequences, and <em>bla</em>_CTX-M gene fragments to generate and express recombinant plasmids containing these fragments in <em>Escherichia coli.</em> We then assessed the cephalosporin resistance phenotype of recombinant clones using the E-TEST method.</div></div><div><h3>Results</h3><div>The carriage rate of IS<em>Ecp1</em> in cephalosporin-resistant <em>Shigella</em> was 90.09%, with an IS<em>Ecp1</em>-CTX-M element carriage rate of 67.19%, and was significantly higher in the <em>bla</em>_CTX-M-9 group than that in the <em>bla</em>_CTX-M-1 group. Moreover, <em>E. coli</em> strains containing different combinations exhibited varying bacterial resistance to β-lactam antibiotics, indicating the regulatory role of IS<em>Ecp1</em> and spacers.</div></div><div><h3>Conclusions</h3><div>Our results demonstrate that the configuration of IS<em>Ecp1</em>, spacer sequences, and <em>bla</em>_CTX-M in plasmids fosters a robust resistance phenotype against various cephalosporins, providing new insights into antimicrobial resistance mechanisms and an important basis for developing novel anti-resistance drugs.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 127-134"},"PeriodicalIF":3.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144368917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights into a novel ST17306 Escherichia coli Isolate carrying tet(X4) from an infant with bronchopneumonia in China","authors":"Mengting Luo ,&nbsp;Qiao Li ,&nbsp;Fang He ,&nbsp;Zhengquan Yang","doi":"10.1016/j.jgar.2025.06.010","DOIUrl":"10.1016/j.jgar.2025.06.010","url":null,"abstract":"<div><h3>Objectives</h3><div>Despite the widespread occurrence of <em>tet</em>(X4)-mediated tigecycline resistance in <em>Escherichia coli</em> from animal and environmental sources, its presence in human clinical isolates remains rare. In this study, we report the first whole-genome sequence of a <em>E. coli</em> isolate harboring <em>tet</em>(X4) and belonging to a novel sequence type, recovered from an infant in China.</div></div><div><h3>Methods</h3><div>The complete genome of <em>E. coli</em> Q65 was sequenced using a combination of Illumina NovaSeq 6000 and Oxford Nanopore MinION sequencing platforms. Functional annotation was conducted using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP), and genomic features were further analyzed with a range of bioinformatics tools.</div></div><div><h3>Results</h3><div>The genome of <em>E. coli</em> strain Q65 is 5 442 192 bp in length and encodes 5089 proteins. Strain Q65 was classified as sequence type ST17306, a novel member of the ST69 clonal complex, and was assigned the serotype O17:H18. Q65 exhibited multidrug resistance, carrying 14 antimicrobial resistance genes, including <em>tet</em>(X4). The <em>tet</em>(X4) gene was located on a 192 048 bp hybrid plasmid belonging to the IncFIA(HI1)/IncHI1B(R27)/IncHI1A replicon types. A search of the NCBI database revealed similar <em>tet</em>(X4)-carrying hybrid plasmids present in various <em>Enterobacteriaceae</em> species, suggesting that such plasmids may play a key role in mediating the horizontal transfer of <em>tet</em>(X4).</div></div><div><h3>Conclusion</h3><div>This study presents the first complete genome sequence of a <em>tet</em>(X4)-positive, multidrug-resistant <em>E. coli</em> strain belonging to the novel sequence type ST17306, isolated from a Chinese infant with bronchopneumonia. Continuous global surveillance of the dissemination of <em>tet</em>(X4)-harboring strains is crucial to monitor and control the potential public health threat.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 146-148"},"PeriodicalIF":3.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144368916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustained outbreak of blaVIM-1 harbouring pathogens in an Italian tertiary hospital: Genomic insights into persistence and spreading","authors":"Francesca Saluzzo ,&nbsp;Federico Di Marco ,&nbsp;Virginia Batignani ,&nbsp;Kiarash Moghaddasi ,&nbsp;Franca Averara ,&nbsp;Silvia Bracco ,&nbsp;Gabriele Del Castillo ,&nbsp;Claudio Farina ,&nbsp;Daniela Maria Cirillo","doi":"10.1016/j.jgar.2025.06.007","DOIUrl":"10.1016/j.jgar.2025.06.007","url":null,"abstract":"<div><h3>Objectives</h3><div>We investigated an outbreak of <em>bla_VIM</em> harbouring pathogens lasting over a year, affecting patients in an intensive care unit (ICU) in a tertiary hospital in Bergamo, Italy. To identify transmission routes of pathogens, we combined classical epidemiological investigation with the molecular identification of the mobile genetic elements (MGEs), integrons, and resistance cassettes that could have played a role in the outbreak persistence.</div></div><div><h3>Methods</h3><div>Short and long reads sequencing were performed. Transmission, resistome, and virulome analysis were conducted, as well as the identification of plasmids and integrons harbouring <em>bla</em>_VIM-1.</div></div><div><h3>Results</h3><div>Forty-four <em>bla_VIM</em> harbouring isolates, including Enterobacterales and <em>Pseudomonadaceae</em>, were identified from patients and environmental samples. The strains exhibited diverse resistomes and virulomes, with high mortality rates among infected patients. The performed assembly analysis did not support the hypothesis of interspecies plasmid transmission, but, nonetheless, genomic analysis revealed a consistent presence of a class 1 integron (IntI1) in all the analysed strains. The identification of the same IntI1 in clinical and environmental samples suggests a potential reservoir for resistant strains in the hospital environment.</div></div><div><h3>Conclusions</h3><div>These findings emphasize the need for comprehensive infection control measures, including genomic-based surveillance, to address MGEs’ horizontal spreading and persistence in the healthcare setting. © 2025 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 111-115"},"PeriodicalIF":3.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology of aminoglycosides resistance and phenotypic detection of aac(6’)-Ib-cr gene in ESBL-producing Enterobacterales in febrile urinary tract infection in children","authors":"Audrey Baron ,&nbsp;Fouad Madhi ,&nbsp;Philippe Bidet ,&nbsp;Corinne Levy ,&nbsp;Robert Cohen ,&nbsp;Yasmine Benhadid-Brahmi ,&nbsp;Camille Jung ,&nbsp;Elsa Sobral ,&nbsp;Kevin La ,&nbsp;Stéphane Bonacorsi ,&nbsp;André Birgy","doi":"10.1016/j.jgar.2025.06.008","DOIUrl":"10.1016/j.jgar.2025.06.008","url":null,"abstract":"<div><h3>Objectives</h3><div>ESBL-producing Enterobacterales (ESBL-E) poses a growing challenge in managing febrile urinary tract infections (FUTIs) in children, leaving amikacin as a good probabilistic once-daily and outpatient treatment option. This epidemiological study investigates aminoglycoside resistance patterns among pediatric ESBL-E isolates, with a particular focus on the <em>aac</em>(6’)-<em>Ib-cr</em> gene—a common resistance determinant that elevates amikacin MICs and may compromise its efficacy. We also aimed to evaluate a phenotypic method to detect <em>aac</em>(6’)-<em>Ib-cr</em> in clinical practice.</div></div><div><h3>Methods</h3><div>We studied 251 ESBL-E from pediatric FUTIs collected in 24 centers. All underwent whole-genome sequencing to determine aminoglycoside resistance genes. Antimicrobial susceptibility was determined by disk diffusion and E-test. Amikacin MICs were measured at standard (10^5 CFU/mL) and high (10^7 CFU/mL) inocula, and interpreted according to EUCAST 2024 breakpoints.</div></div><div><h3>Results</h3><div>Clinically relevant aminoglycoside-resistance genes were identified in 54.2% of isolates, although only 3.6% were resistant to amikacin. Among the isolates, 31.1% (78/251) carried <em>aac</em>(6’)-Ib-cr, co-occurring with other aminoglycoside-resistance genes in 94.9% of cases. Strains harboring aac(6’)-Ib-cr displayed higher amikacin MICs (median 8 mg/L vs 2 mg/L; p&lt;0.001) and became resistant at high inoculum. We propose a simple phenotypic algorithm combining “cliff-like” amikacin inhibition-zone edges and tobramycin disk diameters (&lt;16 mm), correctly identifying 99% of our aac(6’)-Ib-cr-positive strains.</div></div><div><h3>Conclusion</h3><div>The low amikacin resistance in ESBL-E supports its continued use as a reliable empiric treatment for pediatric FUTIs. However, the aac(6’)-Ib-cr gene can raise amikacin MICs, potentially compromising efficacy in high-inoculum infections despite EUCAST susceptibility. Our phenotypic algorithm helps detect it and guide treatment decisions for pediatric FUTIs.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic dissection of nosocomial multiclonal-outbreak by carbapenem-resistant Pseudomonas aeruginosa strains in a tertiary children’s hospital in Japan","authors":"Akihiko Shimizu ,&nbsp;Jun Kurushima ,&nbsp;Koichi Tanimoto ,&nbsp;Naoko Tomie ,&nbsp;Yusuke Hashimoto ,&nbsp;Takahiro Nomura ,&nbsp;Natsuko Ota ,&nbsp;Hidetada Hirakawa ,&nbsp;Haruyoshi Tomita","doi":"10.1016/j.jgar.2025.05.026","DOIUrl":"10.1016/j.jgar.2025.05.026","url":null,"abstract":"<div><h3>Objective</h3><div>Carbapenem-resistant <em>Pseudomonas aeruginosa</em> (CRPA) is an important antimicrobial-resistant bacterial pathogen. We observed an increase in the detection rate of CRPA isolates at a tertiary children’s hospital in Japan. We suspected an outbreak of a clonal CRPA strain and conducted a genetic study to elucidate the resistance mechanism and possible spread of CRPA strains.</div></div><div><h3>Methods</h3><div>For 25 CRPA isolates, antimicrobial susceptibilities to eight antibiotics that are generally used for <em>P. aeruginosa</em> infection were tested. Whole genomes of the isolates were sequenced via next-generation sequencing analysis and subjected to comparative lineage analysis, AMR gene profiling, and Bayesian inference of transmission routes.</div></div><div><h3>Results</h3><div>The genomes of the strains were not monoclonal but comprised multiple lineages with various sequence types. The CRPA isolates had a dysfunctional <em>oprD</em> gene, which is an importer of carbapenem; however, specific nucleotide mutations varied by lineage. Furthermore, administration of antimicrobials against CRPA, such as piperacillin, ceftazidime, and aztreonam, was immediately followed by resistance to these antimicrobials, and in these cases, some unique single nucleotide variants in lasR were identified.</div></div><div><h3>Conclusions</h3><div>Molecular epidemiological data have revealed a multilineage outbreak of <em>P. aeruginosa</em> strains that independently developed carbapenem resistance. This emphasises the rapid evolution of antimicrobial resistance in <em>P. aeruginosa</em> in response to the antimicrobial doses administered to patients who are hospitalised.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 89-94"},"PeriodicalIF":3.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urinary tract infections caused by Gram-negative bacteria in elderly hospitalized patients: Epidemiology, clinical features and outcomes in the era of antimicrobial resistance","authors":"Gabriele Giuliano ,&nbsp;Giulia Hankache ,&nbsp;Margherita Sambo ,&nbsp;Maria Grazia Cusi ,&nbsp;Pietro Enea Lazzerini ,&nbsp;Luigi Gennari ,&nbsp;Massimiliano Fabbiani ,&nbsp;Francesca Montagnani ,&nbsp;Mario Tumbarello","doi":"10.1016/j.jgar.2025.06.006","DOIUrl":"10.1016/j.jgar.2025.06.006","url":null,"abstract":"<div><h3>Objective</h3><div>Urinary tract infections (UTI) are among the most common infections in elderly hospitalized patients, often caused by multi-drug resistant (MDR) organisms and characterized by poor clinical outcomes. This study aimed to evaluate the clinical characteristics, microbiology, treatment patterns, and predictors of mortality in elderly hospitalized patients with UTI, with a focus on infections caused by MDR Gram-negative bacteria (MDR-GNB).</div></div><div><h3>Methods</h3><div>A retrospective analysis was conducted on 171 patients hospitalized between January 2022 and December 2023 at a large academic hospital in Siena, Italy. Data on demographics, comorbidities, infection characteristics, antimicrobial resistance, treatments, and outcomes were collected. Risk factors for MDR-GNB UTI and predictors of 14-d mortality were identified through univariable and multivariable analyses.</div></div><div><h3>Results</h3><div>Of 171 patients (median age: 82 y), 106 (62.0%) had a catheter-associated UTI, and 105 (61.4%) had a healthcare-associated UTI. MDR-GNB were isolated in 68.4% of cases. Common pathogens included <em>Escherichia coli</em> (74/171, 43.3%), <em>Klebsiella</em> spp. (39/171, 22.8%) and <em>Pseudomonas aeruginosa</em> (33/171, 19.3%). Among Enterobacterales 35.1% were Extended-spectrum β-lactamases-producers, 3.7% carried <em>Klebsiella pneumoniae</em> carbapenemase, and 3.0% metallo-β-lactamases. Overall, the 14-d mortality rate was 12.9%. Predictors of 14-d mortality included septic shock, infections caused by <em>Providencia stuartii,</em> infections caused by New Delhi metallo-β-lactamase-producing <em>K. pneumoniae</em>, and inappropriate empirical antibiotic therapy.</div></div><div><h3>Conclusions</h3><div>UTI significantly affect hospital length of stay and mortality in elderly patients. In the current context, resistance mechanisms including <em>K. pneumoniae</em> carbapenemase and metallo-β-lactamases production, must be considered when managing these infections. Prompt recognition of risk factors for infections caused by MDR organisms and optimized antimicrobial strategies are essential to improve outcomes in this vulnerable population.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 116-126"},"PeriodicalIF":3.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights of the co-existence of blaOXA-23, blaOXA-91, blaNDM-1 harboring carbapenem-resistant Acinetobacter Baumannii isolates from the intensive care units environment in Shanghai","authors":"Xiao Wang ,&nbsp;Bing Zhao ,&nbsp;Yuqing Zhou ,&nbsp;Yue Zhang ,&nbsp;Tongsheng Xu ,&nbsp;Yuan Zhuang ,&nbsp;Min Chen ,&nbsp;Lipeng Hao ,&nbsp;Yifeng Shen ,&nbsp;Jun Feng","doi":"10.1016/j.jgar.2025.05.025","DOIUrl":"10.1016/j.jgar.2025.05.025","url":null,"abstract":"<div><h3>Objectives</h3><div>This study assessed the transmission, prevalence and genomic characteristics of carbapenem-resistant <em>Acinetobacter Baumannii</em> (CARB) isolated from object surfaces and healthcare workers' hands in seven hospitals of Shanghai in 2019 (pre-COVID pandemic) and 2023 (post-COVID pandemic).</div></div><div><h3>Methods</h3><div>CRAB isolates were tested for antimicrobial susceptibility using the broth microdilution method and whole-genome sequencing. Antimicrobial resistance genes, Multilocus Sequence Typing (MLST), polysaccharide capsule and plasmid migration were evaluated based on tools such as VFDB database, PubMLST, Kaptive, MOB-suite, etc.</div></div><div><h3>Results</h3><div>Of 3,156 samples collected, 130 strains of CRAB were isolated. The prevalence of CRAB in the ICU setting was significantly lower in 2023 (2.44%) than in 2019 (5.60%). Objects that come into direct contact with patients served as a primary source (43.85%). CRAB isolates exhibited higher resistance to β-lactamase antibiotics but lower to minocycline (2.31%), tigecycline (3.08%) and polymyxin B (2.31%). Five ST_Pasteur and eleven ST_Oxford were identified, predominantly ST2_Pasteur (93.6%) and ST208_Oxford (40%). Two novel sequence types (STs) named ST3329 and ST3331 were found in the surface of objects such as door handles. Notably, two CARB isolates (designated 2023-AB023 and 2023-AB033) co-harboring the <em>bla</em>_OXA-23, <em>bla</em>_OXA-91, <em>bla</em>_NDM-1 were found to be high genomic similarity to DETAB-R21 previously isolated from an ICU patient's oral swab with high environmental adaptability, reported in August 2022 in Zhejiang Province of China.</div></div><div><h3>Conclusions</h3><div>The resistance rate of CRAB was slightly lower in 2023 compared to 2019. However, the emergence of pan-drug-resistant CRAB, along with the coexistence of carbapenemase-producing strains (OXA-23, OXA-51-like, and NDM-1) in 2023, underscore the dynamic progression of antimicrobial resistance (AMR) in this pathogen. These findings suggest a potential epidemiological shift characterized by clonal diversification alongside persistent carbapenemase-driven resistance mechanisms.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 72-80"},"PeriodicalIF":3.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and genomic characterization of Enterobacter kobei ST56 co-carrying mcr-9, blaNDM-1, and blaCTX−M-3 resistance genes","authors":"Bingyou Yin ,&nbsp;Ruishan Liu ,&nbsp;Xinjun Hu","doi":"10.1016/j.jgar.2025.06.001","DOIUrl":"10.1016/j.jgar.2025.06.001","url":null,"abstract":"<div><h3>Objectives</h3><div>This study reports the complete genome sequence of <em>Enterobacter kobei</em> (<em>E. kobei</em>) L5089hy of ST56 isolated from China, carrying <em>mcr-9, bla</em>_NDM-1, and <em>bla</em>_CTX−M-3. It aims to investigate its molecular characteristics and antibiotic resistance mechanisms.</div></div><div><h3>Methods</h3><div>The <em>E. kobei</em> strain was isolated from the faecal sample of an in-patient in a hospital in Hangzhou, China. Whole-genome sequencing was performed using Oxford Nanopore technology, and the genome was assembled with Unicycler version 0.4.7. The strain was identified by 16S rRNA sequencing. The genome sequence was uploaded to ResFinder version 4.1 to identify antimicrobial resistance genes (ARGs). Multilocus sequence typing (MLST) was used to determine the sequence type of the strain. Genome annotation and plasmid type identification were carried out with the aid of the Proksee and PlasmidFinder databases. An antimicrobial susceptibility test (AST) was conducted on the strain. Circular mapping of multiple plasmid comparisons was drawn by BLAST Ring Image.</div></div><div><h3>Results</h3><div><em>E. kobei</em> L5089hy consists of 1 chromosome and 5 plasmids. The <em>mcr-9</em> gene is located on the chromosome, <em>bla</em>_NDM-1 is on the IncFII(Yp) plasmid, and <em>bla</em>_CTX−M-3 is present on both the chromosome and the IncN2 plasmid. The MLST typing result shows that L5089hy is of the ST56 type. AST indicates that the strain is resistant to a variety of antibiotics. However, despite carrying the <em>mcr-9</em> gene, it is sensitive to colistin (minimum inhibitory concentration = 1 μg/mL).</div></div><div><h3>Conclusions</h3><div>This study emphasizes the necessity for monitoring <em>E. kobei</em>© 2025 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"44 ","pages":"Pages 186-188"},"PeriodicalIF":3.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}