Journal of global antimicrobial resistance最新文献

{"title":"Molecular characterization of methicillin-susceptible/resistant Staphylococcus aureus (MSSA/MRSA) from bloodstream infections in northern Japan : the dominance of CC1-MRSA-IV, the emergence of human-associated ST398 and livestock-associated CC20 and CC97 MSSA.","authors":"Meiji Soe Aung, Masako Osada, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Mina Hirose, Masahiko Ito, Kazuki Yamada, Kousuke Tada, Nobumichi Kobayashi","doi":"10.1016/j.jgar.2024.12.010","DOIUrl":"https://doi.org/10.1016/j.jgar.2024.12.010","url":null,"abstract":"<p><strong>Objectives: </strong>Staphylococcus aureus is a major cause of bloodstream infections. The recent epidemiological features and antimicrobial resistance trend were analyzed for methicillin-resistant and susceptible S. aureus (MRSA/MSSA) isolates from blood samples in northern Japan.</p><p><strong>Methods: </strong>S. aureus isolates from blood culture were screened by MALDI-TOF and genotyped by the schemes of MLST, coa, agr, spa, and SCCmec types. Antimicrobial resistance genes and virulence factors were detected by multiplex/uniplex PCR. Antimicrobial susceptibility was examined using a broth microdilution test.</p><p><strong>Results: </strong>A total of 301 isolates (163 MRSA and 138 MSSA) were isolated from bloodstream infections in 2023 (From Apr. to Dec.). The MRSA isolates were classified into three groups, i.e., CC1-SCCmec-IV (CC1-IV) (52%), CC5-II (36%), and CC8-IV (12%). The prevalence of CC1 was significantly higher than those in our previous studies (2017-2021). Four CC8-IVa isolates with PVL genes on ΦSa2usa were considered to be the USA300 clone (ST8/spa-t008/coa IIIa/agr I) or its variants that were genotyped as those closely related to ST8/t008 or lacking ACME. In contrast, MSSA was genetically highly divergent and classified into 22 STs, with CC1 (ST1, ST188) being the most common (25%). It was notable that 29 MSSA isolates (21%) were classified into livestock-associated (LA) genotypes, ST20, ST97, and CC398 (ST398, ST291). Genetic characterization of the CC398 isolates suggested that these belong to human-adapted MSSA clones.</p><p><strong>Conclusions: </strong>The present study revealed the increasing trend of CC1 MRSA surpassing CC5, and the emergence of MSSA representing human-adapted CC398, and LA types ST97 and ST20 from bloodstream infections in Japan.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel allelic variants of bla_OXA-48-like carried on IncN₂ and IncC₂ plasmids isolated from clinical cases in Argentina. In vivo emergence of bla_OXA-567.","authors":"Juan Manuel de Mendieta, Denise De Belder, Nathalie Tijet, Barbara Ghiglione, Roberto G Melano, Melina Rapoport, Pablo Power, Adriana Di Bella, Estefanía Biondi, Fernando Pasterán, Alejandra Corso, Gomez A Sonia","doi":"10.1016/j.jgar.2024.12.008","DOIUrl":"https://doi.org/10.1016/j.jgar.2024.12.008","url":null,"abstract":"<p><strong>Background: </strong>The OXA-48-like enzymes are members of the class D β-lactamases, primarily detected in Enterobacterales, with the capacity to hydrolyze carbapenems. The allelic variant bla_OXA-163, which has low hydrolytic activity towards carbapenemes, was detected in Argentina in 2011 and spread successfully since then, giving sporadic origin to novel local variants.</p><p><strong>Aim: </strong>To study the phenotypic profile and the dissemination strategies of two novel OXA enzymes, bla_OXA-438 and bla_OXA-567, harbored in Escherichia coli M17224 and Klebsiella pneumoniae M21014, isolated from two pediatric patients.</p><p><strong>Methods: </strong>MICs were performed to determine the phenotypic profile of the clinical isolates, transcojugants and transformant cells. Biparental conjugation, PCR, Sanger and whole genome sequencing were performed to determine the complete genetic characteristics of the plasmids.</p><p><strong>Results: </strong>Both isolates were found resistant to carbapenems and susceptible to ceftriaxone. bla_OXA-438 was located on an IncN₂ plasmid of 69 Kb while bla_OXA-567 on an IncC₂ plasmid of 175 Kb, both transferable by biparental conjugation. The close genetic environment of the bla_OXA genes suggests a common origin, likely involving mobile genetic elements. Finally, the clinical case of M21014 revealed previous infections of the patient with two genetically related K. pneumoniae ST6838, that carried bla_OXA-163 on IncC₂ plasmid with equal size and genetic hallmarks than that of M21014, providing strong evidence for the intrapatient emergence of bla_OXA-567. CONCLUSIONS: This research underscores the need for ongoing surveillance and integral studies to understand the emergence, biochemistry and dissemination capacity of OXA enzymes with the overarching aim to halt their spread.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of molecular markers of chloroquine resistance in malaria parasites in East Africa: a systematic review and meta-analysis.","authors":"Wagaw Abebe, Amare Mekuanint, Zelalem Asmare, Dagmawi Woldesenbet, Yenesew Mihret, Abebaw Setegn, Tadele Emagneneh","doi":"10.1016/j.jgar.2024.12.019","DOIUrl":"https://doi.org/10.1016/j.jgar.2024.12.019","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Malaria is a serious global public health problem, which is caused by genus Plasmodium. Resistance of the human malaria parasite to antimalarial drugs is a public health concern in malaria endemic countries. Chloroquine is resistant for both P. vivax and P. falciparum. Chloroquine resistance is understood throughout all of Africa's P. falciparum endemic regions. Molecular markers play a crucial role in tracking and understanding the prevalence of antimalarial drug resistance. Currently, there is inadequate information on the prevalence of molecular markers of chloroquine resistance in malaria parasites.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;This systematic review and meta-analysis aimed to determine the pooled prevalence of molecular markers of chloroquine resistance in malaria parasites in East Africa.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Systematic search was performed to retrieve articles from PubMed, Scopus, Science Direct, and the Google Scholar search engine. Twenty potential studies that provided important data on markers of chloroquine resistance in malaria parasites were systematically reviewed and analyzed. Five antimalarial drug resistance markers of chloroquine resistance were extracted separately into Microsoft Excel and analyzed using STATA 17.0. The Inverse of variance (I&lt;sup&gt;2&lt;/sup&gt;) was done to evaluate heterogeneity across studies. The funnel plot and the Egger's test were used to determine the existence or absence of publication bias. A trim-and-fill meta-analysis was carried out to generate a bias-adjusted effect estimate. A random effect model was used to determine the pooled prevalence of molecular markers associated with chloroquine resistance in malaria parasites. Subgroup analysis was performed based on country and year of publication.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A total of 20 studies were included for this systematic review and meta-analysis. The molecular markers like K76T, 76T, N86Y, Y184F, and 86Y were selected for meta-analysis. From this meta-analysis, the pooled prevalence of K76T, 76T, N86Y, Y184F, and 86Y was 34.5 %, 47.3 %, 43.8 %, 58.3 %, and 29.2 %, respectively. After adjusting for publication bias, the estimated pooled prevalence of K76T, 76T, N86Y, Y184F, and 86Y were 34.5 %, 47.3 %, 43.8 %, 58.3 %, and 29.2 %, respectively. Meta-analysis showed a significant difference in all molecular marker prevalence like K76T and 86Y among studies on year of publication except 76T, N86Y, and Y184F. In addition, the meta-analysis showed a significant difference in all molecular marker prevalence like K76T, 76T, N86Y, Y184F, and 86Y among studies at the country level.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;The findings of this systematic review and meta-analysis concerning the molecular markers of chloroquine resistance of malaria parasites in East Africa revealed a significant prevalence of antimalarial drug resistance markers of chloroquine. As a result, continued surveillance and monitoring of ","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends of Mycoplasma genitalium infections in Berlin, Germany, 2017-2023.","authors":"Roger Dumke, Tobias Glaunsinger","doi":"10.1016/j.jgar.2024.12.020","DOIUrl":"10.1016/j.jgar.2024.12.020","url":null,"abstract":"<p><strong>Objective: </strong>The cell wall-less species Mycoplasma genitalium is a sexually transmitted pathogen with a strong tendency to acquire resistance. Current knowledge on trends of resistance rates and differences between the at-risk population of men who have sex with men (MSM) and heterosexual patients, as well as on circulating genotypes in both groups, is limited.</p><p><strong>Methods: </strong>Between August 2017 and December 2023, M. genitalium strains in 373 samples from patients (MSM: n = 269, non-MSM: n = 104) consulting at a specialized sexually transmitted infection practice in Berlin, Germany, were characterized by molecular methods to detect the presence of mutations associated with macrolide (23S rRNA) and quinolone resistance (parC), and to determine the MgpB strain type.</p><p><strong>Results: </strong>Overall, 37.5% of MSM and 30.8% of heterosexual patients carrying M. genitalium were asymptomatic. Among MSM, the rate of macrolide resistance remained relatively constant during the investigation period (mean: 85.9% of strains), whereas quinolone resistance (mean: 19.7%% of strains) increased from 6.8% (2017) to approximately 38% (2021-2023). In contrast, mean resistance rates of 42.2% for macrolides and 12.5% for quinolones were measured in strains from heterosexual patients. The most common MgpB strain types were types 4 (MSM: 38.4%) and 7 (non-MSM: 16.7%).</p><p><strong>Conclusions: </strong>The results of this study confirm a constantly high rate of macrolide-resistant M. genitalium strains and a trend of increased quinolone resistance among MSM in an urban environment. Despite lower rates, the percentage of resistant strains in heterosexual patients has also reached an alarming extent. The determination of MgpB strain types provides insights into the distribution of genotypes of an important agent of sexually transmitted infections in both population groups.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":"29-34"},"PeriodicalIF":3.7,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and genomic characterization of Klebsiella pneumoniae infections in Dhaka, Bangladesh.","authors":"Zannat Kawser, Sushmita Sridhar, Sanchita Kar, Tanbir Habib, Sharmin Akter Mukta, Kasrina Azad, Neyamul Hasan, Umme Kulsum, Abu Bakar Siddik, Saikt Rahman, Nusrat Noor Tanni, Maherun Nesa, Ashlee M Earl, Colin J Worby, Sarah E Turbett, S M Shamsuzzaman, Jason B Harris, Firdausi Qadri, Regina C LaRocque","doi":"10.1016/j.jgar.2024.12.016","DOIUrl":"10.1016/j.jgar.2024.12.016","url":null,"abstract":"<p><strong>Background: </strong>Klebsiella pneumoniae (Kpn), a WHO priority pathogen with high rates of antimicrobial resistance (AMR), has emerged as a leading cause of hospital acquired pneumonia and neonatal sepsis.</p><p><strong>Objective: </strong>We aimed to define the clinical characteristics of a cohort of patients with Kpn infection in Dhaka, Bangladesh and to perform phenotypic and genetic characterization of the associated isolates.</p><p><strong>Methods: </strong>We retrospectively extracted clinical data about patients at Dhaka Medical College Hospital from whom Klebsiella spp was isolated from a clinical specimen collected between February and September 2022. We used standard microbiologic techniques to evaluate AMR and whole-genome sequencing (WGS) to assess dominant lineages, common capsular (K) and O-polysaccharide (O) antigen types, and AMR and virulence genes.</p><p><strong>Results: </strong>Ninety-eight patients were included, with diagnoses of pneumonia (38/98, 39 %), wound infection (29/98, 31 %), urinary tract infection (29/98, 31 %) and bacteremia (2/98, 2 %). We tested isolates for susceptibility to eight classes of antibiotics. Of the 98 isolates, 41 % were multidrug resistant (MDR), 15 % were extensively drug resistant (XDR), and 16 % were pan-drug resistant (PDR). Three isolates (3 %) were resistant to polymyxin B. Outcome data were available for 46 patients; 4 patients (8 %) died from infections caused by PDR (n = 2), XDR (n = 1), and MDR isolates (n = 1). WGS revealed a high degree of genomic diversity, with multiple sequence types (STs), O-types and K-types represented; ST16:K81:OL101 and ST43:K30:O1 were the most prevalent.</p><p><strong>Conclusion: </strong>Our findings suggest alarming levels of AMR among Kpn isolates in Bangladesh and a critical need for improved treatment modalities and vaccine development.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":"52-58"},"PeriodicalIF":3.7,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of novel FosX family determinants from diverse environmental samples.","authors":"Nicolas Kieffer, Maria-Elisabeth Böhm, Fanny Berglund, Nachiket P Marathe, Michael R Gillings, D G Joakim Larsson","doi":"10.1016/j.jgar.2024.12.018","DOIUrl":"10.1016/j.jgar.2024.12.018","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to identify novel fosfomycin resistance genes across diverse environmental samples, ranging in levels of anthropogenic pollution. We focused on fosfomycin resistance, and given its increasing clinical importance, explored the prevalence of these genes within different environmental contexts.</p><p><strong>Methods: </strong>Metagenomic DNA was extracted from wastewater and sediment samples collected from sites in India, Sweden, and Antarctica. Class 1 integron gene cassette libraries were prepared, and resistant clones were selected on fosfomycin-supplemented media. Long-read sequencing was performed followed by bioinformatics analysis to identify novel fosfomycin resistance genes. The genes were cloned and functionally characterized in E. coli, and the impact of phosphonoformate on the enzymes was assessed.</p><p><strong>Results: </strong>Four novel fosfomycin resistance genes were identified. Phylogenetic analysis placed these genes within the FosX family, a group of metalloenzymes that hydrolyse fosfomycin without thiol conjugation. The genes were subsequently renamed fosE2, fosI2, fosI3, and fosP. Functional assays confirmed that these genes conferred resistance to fosfomycin in E. coli, with MIC ranging from 32 μg/ml to 256 μg/ml. Unlike FosA/B enzymes, these FosX-like proteins were resistant to phosphonoformate inhibitory action. A fosI3 homolog was identified in Pseudomonas aeruginosa, highlighting potential clinical relevance.</p><p><strong>Conclusions: </strong>This study expands the understanding of fosfomycin resistance by identifying new FosX family members across diverse environments. The lack of phosphonoformate inhibition underscores the clinical importance of these poorly studied enzymes, which warrant further investigation, particularly in pathogenic contexts.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":"8-14"},"PeriodicalIF":3.7,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular identification and antimicrobial resistance profiling of pathogenic E. coli isolates from smallholder livestock households in Central Ethiopia.","authors":"Wagaw Sendeku Chekole, Tesfaye Sisay Tessema, Susanna Sternberg-Lewerin, Ulf Magnusson, Haileeyesus Adamu","doi":"10.1016/j.jgar.2024.12.022","DOIUrl":"10.1016/j.jgar.2024.12.022","url":null,"abstract":"<p><p>Escherichia coli of different pathotypes are frequently involved in morbidity and mortality in animals and humans. The study aimed to identify E. coli pathotypes and determine antimicrobial resistance (AMR) profiles in Ethiopian smallholder livestock households. The pathotyping included 198 E. coli isolates identified from human and environmental samples collected from 98 households. AMR profiling was conducted on selected E. coli pathotypes from 89 households, along with known isolates from calf samples obtained from the same households. Morphological and biochemical tests were used to identify presumptive E. coli isolates. DNA was extracted and then singleplex PCR was used to amplify virulence genes. A disc diffusion test was applied for AMR profilings in E. coli pathotypes. Data were evaluated using chi-square tests and logistic regression. Calf (79.8 %) and human (73.7 %) samples were more likely to contain pathotypes (OR 3.2; 95 % CI: 1.7, 5.9; p=0.001 and OR 2.3; 95 % CI: 1.2, 4.1; p=0.008, respectively) than the environmental samples (55.6 %). ETEC (32.3 %) and STEC (15.2 %) were the most common pathotypes detected in the study samples. Out of the 176 isolates selected for AMR profiling, 85 % were resistant to at least one drug and 36 % were multi-drug resistant (MDR). The MDR isolates were found in 44 households, with 11 sharing identical pathotypes and resistance profiles among the different samples. Thus, E. coli strains were likely circulated among humans, animals, and the environment. This in turn calls for a One-health approach to improve antimicrobial usage standards and promote proper waste disposal practices.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":"59-67"},"PeriodicalIF":3.7,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence and genetic characterization of KLUC-3 extended-spectrum β-lactamase-producing Escherichia coli ST95 High-Risk clone causing nosocomial infection in Japan.","authors":"Wataru Hayashi, Shizuo Kayama, Liansheng Yu, Chiemi Tokushige, Makiko Yuki, Yo Sugawara, Koji Yahara, Motoyuki Sugai","doi":"10.1016/j.jgar.2024.12.012","DOIUrl":"10.1016/j.jgar.2024.12.012","url":null,"abstract":"<p><strong>Objectives: </strong>KLUC β-lactamase is a minor extended-spectrum β-lactamase (ESBL) derived from chromosome-encoded cefotaximase in Kluyvera cryocrescens. This study aimed to characterize the genetic context of KLUC-3-producing Escherichia coli and bla_KLUC-3-harboring plasmids and assess nosocomial transmission.</p><p><strong>Methods: </strong>In a national genomic surveillance conducted in 2019 and 2020, KLUC-3-producing E. coli strains (JBEAACH-19-0093 and JBEAACH-19-0210) were recovered from two pediatric inpatients in a Japanese hospital. Short- and long-read sequencing analyses using the HiSeq X Five and GridION were performed to determine the complete genome sequences.</p><p><strong>Results: </strong>JBEAACH-19-0093 and JBEAACH-19-0210 belong to the B2-O1:K1:H7-ST95-fimH41 global high-risk clones and carry virulence genes related to extraintestinal pathogenic and uropathogenic E. coli. Single nucleotide polymorphism analysis showed high homology (13 SNPs) between the strains, suggesting nosocomial transmission. The bla_KLUC-3 gene was flanked by ISEcp1 and Δorf477 on 100-kb IncB/O/K/Z plasmids with a complete sequence identity. Comparative analysis revealed ISEcp1-mediated transposition of bla_KLUC-3 into the IncB/O/K/Z plasmid; the complete plasmid sequence was highly similar to bla_CTX-M- and bla_CMY-2-harboring plasmids from E. coli ST131 isolates. In GenBank database, 26 Enterobacterales harbored bla_KLUC-1 to bla_KLUC-7, particularly in Asia. Among them, the genomic structure of bla_KLUC-orf477/Δorf477 is conserved in 23 strains. In 13 Enterobacterales, except K. cryocrescens, ISEcp1 was inserted upstream of bla_KLUC and 10 strains carried bla_KLUC on the plasmids.</p><p><strong>Conclusion: </strong>This is the first case of nosocomial transmission of KLUC-3 producers outside China. The bla_KLUC-3 emergence in the virulent pandemic lineage ST95 is a public health problem highlighting the need for further investigations to prevent its potential dissemination.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":"35-38"},"PeriodicalIF":3.7,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mobilization of an ICEclc-Like Element as a Potential Mechanism for the Spread of IMP-13 Carbapenemase in Pseudomonas aeruginosa.","authors":"Léa Bientz, Ulysse Guyet, Jennifer Guiraud, Mathieu Metifiot, Mikeldi Moulieras, Sabine Aillerie, Laure Coulange-Mayonnove, Bachir Boureima-Abdou, Alexis Groppi, Macha Nikolski, Cécile Bébéar, Sabine Pereyre, Véronique Dubois","doi":"10.1016/j.jgar.2024.12.006","DOIUrl":"10.1016/j.jgar.2024.12.006","url":null,"abstract":"<p><p>Carbapenem-resistant Pseudomonas aeruginosa is a global public health concern. IMP-13 is a carbapenemase that was described for the first time in 2001 but is often underestimated due to poor hydrolysis of carbapenems and a lack of molecular detection. The aim of this study was to characterize the genetic support of bla_IMP-13 in P. aeruginosa and to assess the ability of mobile genetic elements to disseminate this resistance. A retrospective analysis conducted between 2010 and 2020 revealed eight multiresistant P. aeruginosa isolates by their production of the carbapenemase IMP-13 in Bordeaux. Additionally, three of the studied isolates exhibited high-level resistance to imipenem and imipenem-relebactam that was linked to an insertion sequence in the oprD gene. Successful mating was achieved, and transconjugants and parental clinical isolate genomes were sequenced. All clinical isolates were found to be ST621 strains. The data revealed that bla_IMP-13 was carried on an Integrative and Conjugative Element (ICEclc-like) of 88,589 bp with a 62% GC content harboring 85 CDSs, and was inserted at the tRNA^Gly locus PA0729.1. The ICE was identical in the eight studied clinical isolates and in all the ST621 strains found in the databases. The conjugation rate was low, at approximately 10^-8 transconjugants per donor and ICE transfer appeared to mobilize some adjacent parental genes located immediately downstream of the ICE. In conclusion, these results suggest that even if the spread of bla_IMP-13 is mainly due to an epidemic ST621 clone, the mobilization of a bla_IMP-13-carrying ICEclc-like element is possible and should not be underestimated.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":"44-51"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acinetobacter indicus Coharboring Tet(X6) and bla_NDM-1 Isolated From Slaughterhouse Waste.","authors":"Xiaoqian Long, Feng Lin, Biao Tang, Fengcheng Miao, Zhiyu Li, Yao Shen, Hua Yang, Jiangang Ma","doi":"10.1016/j.jgar.2024.12.004","DOIUrl":"10.1016/j.jgar.2024.12.004","url":null,"abstract":"<p><strong>Objectives: </strong>Acinetobacter indicus is an important pathogen of nosocomial infection. The purpose of this study was to analyze the resistance and transmission of A. indicus strain AIBD14 isolated from slaughterhouse environment.</p><p><strong>Methods: </strong>A total of 96 environmental samples were collected from slaughterhouse. The antimicrobial susceptibility test was carried out by microbroth dilution method and E-test. Whole genome sequencing and bioinformatics analysis of the AIBD14 were performed, then S1-PFGE and southern blot verified the location of bla_NDM-1 and tet(X6).</p><p><strong>Results: </strong>The AIBD14 is resistant to meropenem but susceptibility to tigecycline, and coharboring bla_NDM-1 and tet(X6). The bla_NDM-1 is located on the pAIBD14-NDM-1 that cannot be transferred by conjugation. Specifically, bla_NDM-1 is located on the transposon Tn125, and bla_NDM-1 can be transferred to other species with the help of transposon. The genetic background of bla_NDM-1 is \"ISAba125-bla_NDM-1-ble_MEL-dsbD-cutA-groES-groEL-insE-ISAba125\". pAIBD14-NDM-1 is classified into the GR31 plasmid based on the homology of the repB. Meanwhile, there are two XerC/D-like binding sites on the plasmid, which can mediate the transfer of resistance genes. The tet(X6) gene is located on the chromosome of AIBD14, its downstream is accompanied by the neglected macrolide resistance gene estT, and there is a single copy of the insertion element ISCR2 around tet(X6) as the genetic background \"ISAba4-IS3-hp-hp-tet(X6)-estT-guaA-ISCR2\".</p><p><strong>Conclusions: </strong>This is the first report of the coexistence of tet(X6) and bla_NDM-1 in the A. indicus, and it has the risk of horizontal transfer across multiple species. So strict monitoring the multiple-resistant bacteria in the industrial chain is necessary based on the \"One Heath\".</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":"1-7"},"PeriodicalIF":3.7,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}