Journal of global antimicrobial resistance最新文献

{"title":"The prevalence of carbapenem-resistant Enterobacterales and the emergence of novel ST11-KL30 carbapenem-resistant Klebsiella pneumoniae in Xinjiang, China","authors":"Feng Zhao ,&nbsp;Xiangping Hou ,&nbsp;Gang Sun ,&nbsp;Ying Fu ,&nbsp;Li Wang ,&nbsp;Bingyan Yao ,&nbsp;Xiaochen Liu ,&nbsp;Rui Weng ,&nbsp;Yan Meng ,&nbsp;Jiancang Zhou ,&nbsp;Yan Jiang ,&nbsp;Yunsong Yu ,&nbsp;Qiucheng Shi","doi":"10.1016/j.jgar.2024.10.001","DOIUrl":"10.1016/j.jgar.2024.10.001","url":null,"abstract":"<div><h3>Objectives</h3><div>To address the lack of research on the prevalence of carbapenem-resistant Enterobacterales (CREs) in Xinjiang, China, and elucidate the genomic characteristics of carbapenem-resistant <em>Klebsiella pneumoniae</em> (CRKP) ST11-KL30.</div></div><div><h3>Methods</h3><div>CREs were collected in Xinjiang from 2021 to 2023. The antimicrobial susceptibility testing of carbapenems was performed via agar dilution method. Whole-genome sequencing was completed on the Illumina platform, and subsequent genomic analyses of CRKP, such as sequencing typing, K-locus and O-locus identification, virulence score assessment, and phylogenetic analysis, were performed. The virulence of CRKP isolates was determined <em>in vitro</em> and <em>in vivo</em>, and biofilm formation was assessed by crystal violet staining. Additionally, the virulence plasmid was reconstructed, and the formation of CRKP ST11-KL30 was revealed based on genome data from public database.</div></div><div><h3>Results</h3><div>Eighty-five CRE isolates were collected, among which CRKP was most prevalent (68/85). KPC was the most dominant carbapenemase (60/68) in CRKP, while NDM-type carbapenemase was more prevalent in other species. ST11 was the dominant CRKP clone and was phylogenetically divided into three clusters: ST11-KL64, ST11-KL47 and ST11-KL30. CRKP ST11-KL30 is a novel recombinant clone that harbours a pK2044-like virulence plasmid and can be derived from ST11-KL64 by obtaining an ∼57 kb region from ST29-KL30. Compared to ST11-KL47 and ST11-KL64, ST11-KL30 had lower virulence, but had enhanced biofilm formation.</div></div><div><h3>Conclusions</h3><div>We describe the prevalence of CRE prevalence southern Xinjiang and report the emergence of a region-specific clone. Our findings underscore the potential dissemination of ST11-KL30, which warrants increased monitoring in the future.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 189-195"},"PeriodicalIF":3.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of carbapenem-resistant gram-negative bacilli in Japan using the fully automated bacterial testing device RAISUS S4.","authors":"Yumiko Funashima, Rin Hamabe, Kei Tominaga, Kentaro Wakamatsu, Takahiro Yaguchi, Zenzo Nagasawa, Tsukuru Umemura","doi":"10.1016/j.jgar.2024.09.009","DOIUrl":"10.1016/j.jgar.2024.09.009","url":null,"abstract":"<p><strong>Objective: </strong>We investigated a rapid detection method for carbapenemase-producing gram-negative bacilli (CP-GNR) using meropenem (MEPM) to assess the efficiency of the antimicrobial susceptibility testing.</p><p><strong>Methods: </strong>We used the function that can monitor the growth curve with the resistant bacteria monitoring function (RAISUS S4). Rapid detection of CP-GNR was performed using RAISUS S4 in two types of antimicrobial susceptibility testing, the RAISUS 18-hour method (18-h method) and RAISUS rapid method (rapid method) for Enterobacterales (F-GNR) and non-fermenting gram-negative bacilli (NF-GNR).</p><p><strong>Results: </strong>When F-GNR were based on MEPM MIC ≥ 0.25 µg/mL, CP-GNR were detected with a sensitivity of 100% (58/58) for the 18-h method and 98.3% (57/58) for the rapid method; the shortest detection times were 5.3 and 4.0 h, respectively. When NF-GNR were based on MEPM MIC > 8 µg/mL, it was possible to detect CP-GNR with 100% sensitivity (58/58) in both methods. Furthermore, in the analysis using the 18-h method for monitoring resistant bacteria, when ≥ 2 µg/mL was used as the screening concentration for F-GNR, approximately 50% of the resistant genotypes, NDM, GES, and KPC, were detected in approximately 7 h However, detecting the IMP and VIM took 11-12 h CONCLUSIONS: The 18-h and rapid methods with RAISUS S4 were highly correlated with the results of the microdilution method of CLSI, and CP-GNR detection was rapid using a function that can monitor the growth curve with RAISUS S4.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":"202-208"},"PeriodicalIF":3.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coexistence of seven different carbapenemase producers in a single hospital admission screening confirmed by whole-genome sequencing","authors":"Frieder Fuchs ,&nbsp;Kyriaki Xanthopoulou ,&nbsp;Tessa Burgwinkel ,&nbsp;Rocío Arazo del Pino ,&nbsp;Esther Wohlfarth ,&nbsp;Florian Pavlu ,&nbsp;Ralf M. Hagen ,&nbsp;Paul G. Higgins","doi":"10.1016/j.jgar.2024.09.005","DOIUrl":"10.1016/j.jgar.2024.09.005","url":null,"abstract":"<div><h3>Objective</h3><div>To molecularly characterize several extensively drug-resistant isolates from a single hospital admission screening of a war-injured patient from Ukraine.</div></div><div><h3>Methods</h3><div>Admission screening included swabs from skin, wounds, catheters, nasopharyngeum and rectum. Bacterial identification, antimicrobial susceptibility testing and rapid multiplex PCR assays targeting resistance genes were performed during routine diagnostics. Isolates positive by PCR had their genomes sequenced using short- and long read-platforms (MiSeq and MinION) to confirm species, identify resistance genes and plasmids and investigate clonality with core-genome MLST.</div></div><div><h3>Results</h3><div>Seven Gram-negative pathogens (<em>Acinetobacter baumannii</em> (<em>n</em> = 2; ST78, ST2), <em>Klebsiella pneumoniae</em> (<em>n</em> = 2; ST395), <em>Pseudomonas aeruginosa</em> (<em>n</em> = 1; ST1047), <em>Escherichia coli</em> (<em>n</em> = 1; ST46), <em>Enterobacter cloacae</em> complex (<em>n</em> = 1; ST231)) were molecularly confirmed non-identical. Antimicrobial susceptibility testing showed resistance to carbapenems (7/7 isolates) and last-resort treatment options such as ceftazidime-avibactam (6/7 isolates) and cefiderocol (4/7 isolates). All isolates were colistin susceptible. Sequencing identified the <em>E. cloacae complex</em> as <em>Enterobacter hormaechei</em> subsp<em>. xiangfangensis</em>. Six acquired carbapenemase genes (<em>bla</em>_IMP-1, <em>bla</em>_NDM-1, <em>bla</em>_OXA-48, <em>bla</em>_NDM-5, <em>bla</em>_OXA-23 and <em>bla</em>_OXA-72) were detected<em>.</em> Both <em>A. baumannii</em> isolates differed in sequence type, carbapenemases and cefiderocol susceptibility. Both <em>K. pneumoniae</em> isolates shared sequence type and some resistance genes on an IncR plasmid but were different in core-genome MLST and carbapenemases (OXA-48 or NDM-1). One vancomycin-resistant <em>Enterococcus faecium</em> was also detected (<em>VanA</em>).</div></div><div><h3>Conclusions</h3><div>War-injured patients from Ukraine may carry different clones of multidrug-resistant pathogens with limited treatment options and diverse resistance genes at risk for dissemination. Infection control measures should include early molecular characterization of isolates for detection of routes of transmission.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 184-188"},"PeriodicalIF":3.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of OXA-48 carbapenemase-producing Salmonella enterica in the Netherlands, 2023","authors":"Gijs Teunis ,&nbsp;Maren Lanzl ,&nbsp;Kees T. Veldman ,&nbsp;Roan Pijnacker ,&nbsp;Fabian Landman ,&nbsp;Jeroen Bos ,&nbsp;Michael S.M. Brouwer ,&nbsp;Jenny Schuch ,&nbsp;Karola Waar ,&nbsp;Eelco Franz ,&nbsp;Antoni P.A. Hendrickx","doi":"10.1016/j.jgar.2024.09.008","DOIUrl":"10.1016/j.jgar.2024.09.008","url":null,"abstract":"","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 196-198"},"PeriodicalIF":3.7,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two novel plasmids harbouring the multiresistance gene cfr in porcine Staphylococcus equorum","authors":"Ning Xie ,&nbsp;Tengfei Ma ,&nbsp;Yuan Gao ,&nbsp;Jiani Fu ,&nbsp;Chune Tan ,&nbsp;Zheng Wang ,&nbsp;Zhuo Wang ,&nbsp;Henrike Krüger-Haker ,&nbsp;Andrea T. Feßler ,&nbsp;Congming Wu ,&nbsp;Jianzhong Shen ,&nbsp;Stefan Schwarz ,&nbsp;Yang Wang","doi":"10.1016/j.jgar.2024.09.004","DOIUrl":"10.1016/j.jgar.2024.09.004","url":null,"abstract":"<div><h3>Background</h3><div>The emergence and transmission of the multidrug resistance gene <em>cfr</em> have raised public health concerns worldwide.</div></div><div><h3>Objectives</h3><div>Multidrug-resistant <em>Staphylococcus equorum</em> isolates can pose a threat to public health. In this study, we have characterised the whole-genome of one <em>Staphylococcus equorum</em> isolate harbouring two distinct <em>cfr</em>-carrying plasmids.</div></div><div><h3>Methods</h3><div>Antimicrobial susceptibility testing was performed by broth microdilution. Genomic DNA was sequenced using both the Illumina HiSeq X Ten and Nanopore MinION platforms. <em>De novo</em> hybrid assembly was performed by Unicycler. Genomic data were assessed by in silico prediction and bioinformatic tools.</div></div><div><h3>Results</h3><div><em>Staphylococcus equorum</em> isolate SN42 exhibited resistance or high MICs to linezolid, erythromycin, tetracycline, oxacillin, clindamycin, virginiamycin, tiamulin, chloramphenicol and florfenicol. It carried two <em>cfr</em>-harbouring plasmids: the RepA N-family plasmid pSN42–51 K and the Inc18-family plasmid pSN42–50 K. These two plasmids exhibited low structural similarities to the so far reported <em>cfr</em>-carrying plasmids. Both plasmids harboured an arsenic resistance operon, copper and cadmium resistance genes as well as the lincosamide-pleuromutilin-streptogramin A resistance gene <em>lsa</em>(B). In addition, plasmid pSN42–51 K carried two <em>erm</em>(B) genes for macrolide-lincosamide-streptogramin B resistance, the streptomycin resistance gene <em>ant(6)-Ia</em> as well as mercury resistance genes while pSN42–50 K was associated with the heavy metal translocating P-type ATPase gene <em>hmtp</em>. The co-carriage and co-existence of these antimicrobial resistance and heavy metal resistance genes increases the likelihood of co-selection of the <em>cfr</em>-carrying plasmids.</div></div><div><h3>Conclusion</h3><div>This is the first report of <em>S. equorum</em> carrying two distinct <em>cfr</em>-carrying plasmids, underscoring the need for ongoing surveillance to address the potential dissemination of multi-drug resistance in bacteria from food-producing animals to ensure food safety and public health.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 170-174"},"PeriodicalIF":3.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Occurrence and characteristics of blaOXA-181 - carrying Klebsiella aerogenes from swine in China” [Journal of Global Antimicrobial Resistance 38(2024) 35–41/JGAR_2323]","authors":"Lin Sun ,&nbsp;Nan Meng ,&nbsp;Hanyun Wang ,&nbsp;Zhenyu Wang ,&nbsp;Xinan Jiao ,&nbsp;Jing Wang","doi":"10.1016/j.jgar.2024.08.009","DOIUrl":"10.1016/j.jgar.2024.08.009","url":null,"abstract":"","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Page 92"},"PeriodicalIF":3.7,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524001619/pdfft?md5=893228827e63500e2510674179600f1f&pid=1-s2.0-S2213716524001619-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142271634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age and gender in drug resistance tuberculosis: A cross-sectional case study at a national tuberculosis reference hospital in Nigeria","authors":"Suzie Madaki ,&nbsp;Yusuf Mohammed ,&nbsp;Lawal Dahiru Rogo ,&nbsp;Mustapha Yusuf ,&nbsp;Yazeed Garba Bala","doi":"10.1016/j.jgar.2024.09.002","DOIUrl":"10.1016/j.jgar.2024.09.002","url":null,"abstract":"<div><h3>Objectives</h3><div>Drug resistance in tuberculosis (TB) is a very important public health threat that should not be ignored. Understanding the gender, age, and characteristics of individuals affected by TB (without HIV, diabetes, or hepatitis B complications), particularly in terms of drug resistance or susceptibility, is crucial for effective prevention and management strategies, as most studies focus on TB/HIV co-infection.</div></div><div><h3>Methods</h3><div>A cross-sectional case study of age and gender was carried out in 140 individuals grouped into drug-resistant tuberculosis (DR-TB), drug-susceptible tuberculosis (DS-TB), and apparently healthy controls (AHCs). Data collection was through medical records and a structured questionnaire. Statistical analyses compared age, gender, and selected risk factors across the groups.</div></div><div><h3>Results</h3><div>The mean age of the DR-TB group was 32 years (SD ≈ 2). A total of 80.0% were ≤40 years of age and four times more likely to have DR-TB; 55% were male, with 1.22 times more likelihood of DR-TB in males. The mean age of the DS-TB group was 34 years (SD ≈ 12); 66.7% were ≤40 years old. The odds ratio of DS-TB in males was 2.16. Only 10% of DR-TB enrolees had BCG scars compared with 65% AHCs. A high percentage of the DR-TB group reported handling raw meat (75%) and drinking unpasteurised milk (70%) compared with the DS-TB group.</div></div><div><h3>Conclusions</h3><div>The observed gender disparities and age-related factors, particularly among the DR-TB group, highlight the importance of considering age and gender factors in DR-TB prevention, diagnosis, and treatment. Our findings also highlight the need to bridge gaps in awareness as well as for the prevention of zoonotic TB and issues around effective BCG vaccination and coverage.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 175-183"},"PeriodicalIF":3.7,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete genome of Escherichia coli sequence type 73 with acquired blaTEM-1 and high genotypic virulence load identified in human saliva","authors":"Francesca McDonagh ,&nbsp;Anna Tumeo ,&nbsp;Elaine K. Murray ,&nbsp;Brian Hallahan ,&nbsp;Georgios Miliotis","doi":"10.1016/j.jgar.2024.08.011","DOIUrl":"10.1016/j.jgar.2024.08.011","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Escherichia coli</em> sequence type (ST) 73 is a pandemic lineage of the ExPEC (Extraintestinal Pathogenic <em>E. coli</em>) family associated with conserved virulence. We report the complete genome of a genomically hypervirulent <em>E. coli</em> ST73 strain isolated from the oral cavity of a patient with a diagnosis of treatment resistant schizophrenia and receiving clozapine treatment.</div></div><div><h3>Methods</h3><div><em>E. coli</em> strain GABEEC132 underwent second and third generation sequencing with Illumina and Oxford-Nanopore-Technologies (ONT) platforms. Antibiotic resistance genes (ARGs) and virulence factors (VFs) were bioinformatically identified using the NCBI-AMR-Finder-Plus database and Virulence-Factors-database (VFDB), respectively. To contextualize the genome within a broader epidemiological framework, phylogenetic analysis was conducted using representative genomes of <em>E. coli</em> ST73 O6:H1 (<em>n</em> = 55).</div></div><div><h3>Results</h3><div><em>E. coli</em> strain GABEEC132 was identified as possessing the O6:H1 serotype and classified within the B2 phylogroup. The strain exhibited a high genomic virulence load, encoding for 194 VFs. Additionally, it encoded three ARGs, including an acquired <em>blaTEM-1</em> located on a rep_cluster_2350 8 237 Kb mobilisable plasmid, presenting phenotypic resistance to ampicillin and piperacillin.</div></div><div><h3>Conclusion</h3><div>This report provides novel insights into the oral prevalence of genotypically hypervirulent and drug-resistant <em>E. coli</em> ST73, a pandemic lineage.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 149-152"},"PeriodicalIF":3.7,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The frequency of AmpC overproduction, OprD downregulation and OprM efflux pump expression in Pseudomonas aeruginosa: A comprehensive meta-analysis","authors":"Shaya Alimoghadam ,&nbsp;Arvin Eslami ,&nbsp;Rojina Alimoghadam ,&nbsp;Ibrahim Bahrami Mianrood ,&nbsp;Mehdi Azizmohammad Looha ,&nbsp;Sanaz Khodadadi ,&nbsp;Shervin Shokouhi ,&nbsp;Ilad Alavi Darazam","doi":"10.1016/j.jgar.2024.08.014","DOIUrl":"10.1016/j.jgar.2024.08.014","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Pseudomonas aeruginosa</em> is a major opportunistic pathogen responsible for a wide range of infections. The emergence of antibiotic resistance in this pathogen poses a significant public health challenge. This study aims to conduct a comprehensive meta-analysis of studies conducted in Iran to determine the frequency of key antibiotic resistance mechanisms in <em>Pseudomonas aeruginosa</em> and their association with multidrug-resistant and extensively drug-resistant strains or pandrug-resistant strains.</div></div><div><h3>Methods</h3><div>Systematic database searches encompassing literature up to June 2023 were undertaken. The selected studies centered on OprD downregulation, efflux pump (mexAB-OprM, mexXY-OprM) expression, and AmpC overproduction. Extracted data were synthesised in a meta-analysis for pooled frequency determination of each resistance mechanism.</div></div><div><h3>Results</h3><div>In total, 24 studies were included. OprD downregulation exhibited a pooled frequency of 61%. Efflux pump component frequency ranged from 48% to 77.5%. AmpC overproduction was identified in 29.1% of isolates. Polymyxin B and colistin demonstrated lower antibiotic resistance rates, with pooled frequency of 1% and 1.6%, respectively. Conversely, resistance to other antibiotics ranged widely, with pooled frequency spanning 38.4% to 98.2%.</div></div><div><h3>Conclusions</h3><div>This study underscores the concerning frequency of diverse antibiotic resistance mechanisms in <em>Pseudomonas aeruginosa</em> strains from Iran. Concurrent OprD downregulation, mexAB, mexXY, OprM expression, and AmpC overproduction highlight the urgent need for stringent infection control and prudent antibiotic usage to curb the dissemination of these resistant strains.</div></div><div><h3>PROSPERO</h3><div>CRD42022379311</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 159-169"},"PeriodicalIF":3.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthetic lincosamides iboxamycin and cresomycin are active against ocular multidrug-resistant methicillin-resistant Staphylococcus aureus carrying erm genes","authors":"Camille André ,&nbsp;Kelvin J.Y. Wu ,&nbsp;Andrew G. Myers ,&nbsp;Paulo J.M. Bispo","doi":"10.1016/j.jgar.2024.09.001","DOIUrl":"10.1016/j.jgar.2024.09.001","url":null,"abstract":"<div><h3>Objective</h3><div>Antimicrobial resistance is a global pandemic that poses a major threat to vision health as ocular bacteria, especially methicillin-resistant <em>Staphylococcus aureus</em> (MRSA), are becoming increasingly resistant to first-line therapies. Here we evaluated the antimicrobial activity of new synthetic lincosamides in comparison to currently used antibiotics against clinical ocular MRSA isolates.</div></div><div><h3>Methods</h3><div>Antimicrobial susceptibility testing was performed by broth microdilution for two novel synthetic lincosamides (iboxamycin and cresomycin) and eight comparator antibiotics against a collection of 50 genomically characterised ocular MRSA isolates, including isolates harbouring <em>erm</em> genes (<em>n</em> = 25).</div></div><div><h3>Results</h3><div>Both drugs were active against widespread MRSA clonal complexes CC8 and CC5. The MIC₅₀ and MIC₉₀ of iboxamycin were 0.06 and 2 mg/L, respectively. Cresomycin (MIC₅₀ = 0.06 mg/L) also displayed good activity with an <em>in vitro</em> potency four-fold higher (MIC₉₀ = 0.5 mg/L) than iboxamycin. In isolates harbouring <em>erm</em> genes, MIC₉₀ were &gt;16, 2, and 0.5 mg/L for clindamycin, iboxamycin, and cresomycin, respectively. The <em>in vitro</em> potencies of iboxamycin and cresomycin were similar or higher than that of comparator agents and were not impacted by multidrug-resistance phenotypes or by the presence of <em>erm</em> genes when compared with clindamycin.</div></div><div><h3>Conclusions</h3><div>Our results demonstrate that iboxamycin and cresomycin display potent <em>in vitro</em> activity against ocular MRSA isolates, including multidrug-resistant isolates harbouring <em>erm</em> genes.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 144-148"},"PeriodicalIF":3.7,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}