Journal of global antimicrobial resistance最新文献

{"title":"Activity of mecillinam against USA urinary tract clinical isolates from 2017 to 2020 including isolates resistant to comparator antibiotics","authors":"Stephen Hawser ,&nbsp;Ian Morrissey ,&nbsp;Nimmi Kothari ,&nbsp;Federica Monti ,&nbsp;Anne Santerre Henriksen","doi":"10.1016/j.jgar.2024.08.013","DOIUrl":"10.1016/j.jgar.2024.08.013","url":null,"abstract":"<div><h3>Objectives</h3><div>To support the clinical development of mecillinam and pivmecillinam in the United States for the treatment of complicated and uncomplicated urinary tract infections (UTIs), this study investigated the activity of mecillinam compared with other antibiotics against Enterobacterales isolates from patients with UTIs in the United States during 2017 to 2020. Mecillinam is a first-in-class amidinopenicillin antibiotic, being the only β-lactam to exert its antibacterial activity through exclusive binding to penicillin-binding protein 2. Pivmecillinam is the oral prodrug of mecillinam and is recommended as a first-line therapy by the Infectious Disease Society of America guidelines for uncomplicated UTIs and is approved for the treatment of uncomplicated UTIs in Europe, Canada, and the United States.</div></div><div><h3>Methods</h3><div>A total of 3303 isolates were collected and antimicrobial susceptibility determined according to Clinical Laboratory and Standards Institute (CLSI) guidelines.</div></div><div><h3>Results</h3><div>Susceptibility was highest for fosfomycin (97.1% susceptible) and mecillinam (94.9% susceptible). Against extended-spectrum beta-lactamase (ESBL)-positive bacteria susceptibilities were highest for mecillinam (98.2% susceptible) and fosfomycin (97.3% susceptible) and against ESBL-positive <em>K. pneumoniae</em> only mecillinam and fosfomycin had &gt; 80% susceptibility. Resistance to comparator antibiotics was highest for trimethoprim-sulfamethoxazole (27.1%), followed by ciprofloxacin (19.3%), ceftriaxone (19.2%), and nitrofurantoin (12.1%). Multi-drug-resistant isolates were most susceptible to mecillinam and fosfomycin.</div></div><div><h3>Conclusion</h3><div>The data further support the clinical development and clinical utility of mecillinam. © 2024 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 137-143"},"PeriodicalIF":3.7,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Easy analysis of bacterial whole-genome sequencing data for clinical microbiologists using open-source Galaxy platform: Characterization of ESBL-producing Enterobacterales from bloodstream infections","authors":"Aimé Berwa ,&nbsp;Yvan Caspar","doi":"10.1016/j.jgar.2024.08.012","DOIUrl":"10.1016/j.jgar.2024.08.012","url":null,"abstract":"<div><h3>Objectives</h3><div>Clinical microbiologists require easy-to-use open access tools with graphical interfaces to perform bacterial whole-genome sequencing (WGS) in routine practice. This study aimed to build a bioinformatics pipeline on the open-source Galaxy platform, facilitating comprehensive and reproducible analysis of bacterial WGS data in a few steps. We then used it to characterize our local epidemiology of ESBL-producing <em>Enterobacterales</em> isolated from patients with bacteremia.</div></div><div><h3>Methods</h3><div>We built a bioinformatics pipeline consisting of the following sequential tools: Fastp (input data trimming); FastQC (read quality control); SPAdes (genome assembly); Quast (quality control of genome assembly); Prokka (gene annotation); Staramr (ResFinder database) and ABRicate (CARD database) for antimicrobial resistance (AMR) gene screening and molecular strain typing. Paired-end short read WGS data from all ESBL-producing <em>Enterobacterales</em> strains isolated from patients with bacteremia over one year were analysed.</div></div><div><h3>Results</h3><div>The Galaxy platform does not require command line tools. The bioinformatics pipeline was constructed within one hour. It only required uploading fastq files and facilitated systematization of <em>de novo</em> assembly of genomes, MLST typing, and AMR gene screening in one step. Among the 66 ESBL-producing strains analysed, the two most frequent ESBL genes were <em>bla</em>_CTX−M-15 (62.1%) and <em>bla</em>_CTX−M-27 (13.6%).</div></div><div><h3>Conclusions</h3><div>The open-access Galaxy platform provides a graphical interface and easy-to-use tools suitable for routine use in clinical microbiology laboratories without bioinformatics specialists. We believe that this platform will facilitate fast and low-cost analysis of bacterial WGS data, especially in resource-limited settings.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 153-158"},"PeriodicalIF":3.7,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of a persuasive antimicrobial stewardship programme on the appropriateness of surgical antimicrobial prophylaxis in a tertiary care hospital in southern Italy","authors":"Lorenzo Onorato ,&nbsp;Margherita Macera ,&nbsp;Caterina Curatolo ,&nbsp;Viraj Pattapola ,&nbsp;Verdiana Zollo ,&nbsp;Caterina Monari ,&nbsp;Federica Calò ,&nbsp;Pasquale Di Girolamo Faraone ,&nbsp;Ferdinando Russo ,&nbsp;Nicola Coppola","doi":"10.1016/j.jgar.2024.09.003","DOIUrl":"10.1016/j.jgar.2024.09.003","url":null,"abstract":"<div><h3>Background and aim</h3><div>The aim of our study was to evaluate the rate of compliance to institutional guidelines on surgical antimicrobial prophylaxis (SAP) among the surgical wards of our facility and to evaluate the impact of our antimicrobial stewardship programme (ASP) on the adherence rate.</div></div><div><h3>Methods</h3><div>We conducted a cross-sectional study including all 14 surgical wards of our hospital. Eight of these wards participate in a non-restrictive ASP. All patients admitted to one of the wards who had been prescribed SAP were included; the appropriateness of prophylaxis was defined according to the institutional protocols.</div></div><div><h3>Results</h3><div>During the study period, we evaluated 169 patients undergoing a surgical procedure, of whom 105 (62.1%) received SAP. Overall, 10 (10.5%) patients were prescribed unnecessary prophylaxis; among the remaining 95, 30 (31.6%) received appropriate prophylaxis, while 65 (68.4%) were prescribed SAP deemed inappropriate for one or more reasons (38.5% received a drug with a larger spectrum of activity, 9.2% a molecule with a narrower spectrum, 36.9% an incorrect dose and 76.9% a longer duration of prophylaxis). A higher number of patients in the inappropriate prophylaxis group received abdominal surgery (<em>P</em> = 0.001) and were admitted to a ward not participating in the ASP (<em>P</em> &lt; 0.001). At multivariate analysis, being admitted to an ASP unit was the only factor independently related to having received appropriate prophylaxis.</div></div><div><h3>Conclusions</h3><div>A low rate of adherence to local guidelines on SAP was observed, but a non-restrictive ASP can significantly impact the appropriateness of surgical prophylaxis.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 122-127"},"PeriodicalIF":3.7,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of a carbapenem-resistant Aeromonas veronii environmental isolate in the United States co-harboring two carbapenemase genes","authors":"Rafael Estrada,&nbsp;Cristian Ruiz","doi":"10.1016/j.jgar.2024.08.010","DOIUrl":"10.1016/j.jgar.2024.08.010","url":null,"abstract":"<div><h3>Objectives</h3><div>The spread of carbapenem-resistant bacteria (CRB), and especially carbapenemase-producing CRB, is a global public health threat. Among them, <em>Aeromonas</em> species are of increasing concern because these emerging opportunistic pathogens are widespread in the environment and have increasingly been found to be resistant to carbapenems. The aim of this study was to investigate the genome and carbapenem-resistance determinants of <em>Aeromonas veronii</em> SS-M2–3, a highly carbapenem-resistant, carbapenemase-producing, river isolate from California (U.S.).</div></div><div><h3>Methods</h3><div>We first used disk diffusion assays to characterize the susceptibility profile to carbapenems and other antibiotics of <em>A. veronii</em> SS-M2–3. We next used whole-genome sequencing using the Illumina platform and bioinformatics analysis to characterize the resistome of this isolate and identify its carbapenemase genes.</div></div><div><h3>Results</h3><div><em>A. veronii</em> SS-M2–3 was resistant to all carbapenems tested and amoxicillin-clavulanic acid, whereas it was sensitive to cefotaxime and all non-β-lactam antibiotics tested. Whole genome sequencing of this isolate revealed a complex resistome that included multidrug efflux pump genes and three chromosomal β-lactamase genes. These three genes encoded for highly conserved variants (82% to 97% amino acid identity) of the ChpA3 subclass B2 metallo-carbapenemase, OXA-12 class D carbapenemase and the FOX-2 class C β-lactamase. This is the first report of an environmental <em>A. veronni</em> isolate from the U.S. co-harbouring two carbapenemase genes.</div></div><div><h3>Conclusions</h3><div>These findings reveal that natural aquatic environments in the U.S. represent an underappreciated reservoir of carbapenem-resistant <em>Aeromonas veronii</em> isolates that can carry multiple carbapenemase genes.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 119-121"},"PeriodicalIF":3.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome sequence analysis of morphological changes in Haemophilus influenzae after beta-lactam exposure","authors":"Lucia Malisova ,&nbsp;Vladislav Jakubu ,&nbsp;Ibrahim Bitar ,&nbsp;Daniel Krsek ,&nbsp;Helena Zemlickova","doi":"10.1016/j.jgar.2024.08.001","DOIUrl":"10.1016/j.jgar.2024.08.001","url":null,"abstract":"<div><div>This report describes mutations in genes responsible for cell deformities in haemophili under beta-lactam pressure in vitro. Light and transmission electron microscopy confirmed a hypothesis regarding changes in the shape of haemophili that had become more filamentous in the presence of ampicillin (2 mg/L) and cefuroxime (8 mg/L) after 30 days of serial passage. Short-axis size increased by 28% (from 0.767 to 1.06 µm) and long-axis length increased by 54% (from 1 to 2.175 µm). Additionally, whole-genome sequencing analysis (Illumina platform, software PROKKA) revealed a variety of mutations in genes responsible for cell morphology in isolates examined in this study: <em>ftsI</em> (A1576 → C; G1154 → C; T986 → C; G1684 → C), <em>mreB</em> (C476 → T), <em>mreC</em> (A5 → G), <em>mrdA</em> (A1148 → G; C179 → T; G1613 → T), <em>mrdB</em> (T668 → G), <em>mltC</em> (C1016 → T) and <em>rodA</em> (T668 → G). The results of this study indicate that shifts in bacterial shape could play a role in the adaptation of haemophili to a new niche created by beta-lactams as a strategy of antibiotic therapy survival.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 114-118"},"PeriodicalIF":3.7,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of gradient diffusion in vitro susceptibility testing of Aerococcus urinae","authors":"Kendall Kling ,&nbsp;Adil Menon ,&nbsp;Paige Norconk ,&nbsp;David Zhu ,&nbsp;Teresa Zembower ,&nbsp;Chao Qi","doi":"10.1016/j.jgar.2024.07.014","DOIUrl":"10.1016/j.jgar.2024.07.014","url":null,"abstract":"<div><h3>Objective</h3><p><em>Aerococcus urinae</em> antimicrobial susceptibility testing can be performed via broth microdilution with Mueller-Hinton broth supplemented with lysed horse blood. We sought to compare this with the commonly used gradient diffusion method.</p></div><div><h3>Methods</h3><p>We compared broth microdilution with Mueller-Hinton broth supplemented with lysed horse blood and gradient diffusion via Mueller-Hinton agar supplemented with sheep blood for 190 <em>A. urinae</em> isolates against 16 antimicrobials.</p></div><div><h3>Results</h3><p>No antimicrobials demonstrated more than 90% essential and categorical agreement, and fewer than 3% demonstrated major and very major error rates. Trimethoprim-sulfamethoxazole demonstrated an 81% major error rate and ceftriaxone demonstrated a 76% very major error rate. Agar dilution with lysed horse blood was performed for trimethoprim-sulfamethoxazole against 94 isolates and showed 100% susceptibility, consistent with previous studies.</p></div><div><h3>Conclusions</h3><p>Given its limitations in detecting resistant strains, our findings cannot support the routine use of gradient diffusion with Mueller-Hinton agar supplemented with sheep blood for <em>A. urinae</em> in lieu of the Clinical and Laboratory Standards Institute method. Our results suggest that <em>A. urinae</em> is usually susceptible to penicillin, linezolid, tetracycline, and vancomycin. Future studies should evaluate alternative testing methods for clinical microbiology laboratories.</p></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"38 ","pages":"Pages 389-400"},"PeriodicalIF":3.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524001449/pdfft?md5=61136a45ff95860eee2efaed6fe52513&pid=1-s2.0-S2213716524001449-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance rates in hypervirulent Klebsiella pneumoniae strains: A systematic review and meta-analysis","authors":"Masoumeh Beig ,&nbsp;Shadi Aghamohammad ,&nbsp;Nahal Majidzadeh ,&nbsp;Mahshid Khazani Asforooshani ,&nbsp;Niloofar Rezaie ,&nbsp;Sahar Abed ,&nbsp;Elham Hajagha Gholizadeh Khiavi ,&nbsp;Mohammad Sholeh","doi":"10.1016/j.jgar.2024.06.018","DOIUrl":"10.1016/j.jgar.2024.06.018","url":null,"abstract":"<div><h3>Objectives</h3><p>In response to the growing global concerns regarding antibiotic resistance, we conducted a meta-analysis to assess the prevalence of antibiotic resistance in hypervirulent <em>Klebsiella pneumoniae</em> (hvKp) strains.</p></div><div><h3>Methods</h3><p>We conducted a meta-analysis of antibiotic resistance in the hvKp strains. Eligible studies published in English until April 10, 2023, were identified through a systematic search of various databases. After removing duplicates, two authors independently assessed and analysed the relevant publications, and a third author resolved any discrepancies. Data extraction included publication details and key information on antibiotic resistance. Data synthesis employed a random-effects model to account for heterogeneity, and various statistical analyses were conducted using R and the metafor package.</p></div><div><h3>Results</h3><p>This meta-analysis of 77 studies from 17 countries revealed the prevalence of antibiotic resistance in hvKp strains. A high resistance rates have been observed against various classes of antibiotics. Ampicillin-sulbactam faced 45.3% resistance, respectively, rendering them largely ineffective. The first-generation cephalosporin cefazolin exhibited a resistance rate of 38.1%, whereas second-generation cefuroxime displayed 26.7% resistance. Third-generation cephalosporins, cefotaxime (65.8%) and ceftazidime (57.1%), and fourth-generation cephalosporins, cefepime (51.3%), showed substantial resistance. The last resort carbapenems, imipenem (45.7%), meropenem (51.0%) and ertapenem (40.6%), were not spared.</p></div><div><h3>Conclusion</h3><p>This study emphasizes the growing issue of antibiotic resistance in hvKp strains, with notable resistance to both older and newer antibiotics, increasing resistance over time, regional disparities and methodological variations. Effective responses should involve international cooperation, standardized testing and tailored regional interventions.</p></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"38 ","pages":"Pages 376-388"},"PeriodicalIF":3.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524001279/pdfft?md5=72b281471f97e18f7ca83db02c5f32cc&pid=1-s2.0-S2213716524001279-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International Society of Antimicrobial Chemotherapy (ISAC) News and Information Page","authors":"","doi":"10.1016/j.jgar.2024.07.011","DOIUrl":"10.1016/j.jgar.2024.07.011","url":null,"abstract":"","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"38 ","pages":"Pages A1-A2"},"PeriodicalIF":3.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524001425/pdfft?md5=a981dbae8065ee0b18a0978ee3830188&pid=1-s2.0-S2213716524001425-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142173058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virological impact of HIV drug-resistance testing in children, adolescents, and adults failing first-line ART in Tanzania","authors":"Shimba Henerico ,&nbsp;Eric Lyimo ,&nbsp;Abel N. Makubi ,&nbsp;Daniel Magesa ,&nbsp;Bernard Desderius ,&nbsp;Andreas Mueller ,&nbsp;John Changalucha ,&nbsp;Michael Aloyce ,&nbsp;Bence Maziku ,&nbsp;Benson R. Kidenya ,&nbsp;Samuel E. Kalluvya ,&nbsp;Gert Van Zyl ,&nbsp;Wolfgang Preiser ,&nbsp;Stephen E. Mshana ,&nbsp;Christa Kasang","doi":"10.1016/j.jgar.2024.07.018","DOIUrl":"10.1016/j.jgar.2024.07.018","url":null,"abstract":"<div><h3>Background</h3><p>Prospective data on the effectiveness of resistance testing in informing treatment decisions and outcomes in with first-line failure in these settings is limited. This study aimed to assess the virological impact of HIV drug-resistance testing in patients with virological failure in Tanzania.</p></div><div><h3>Methods</h3><p>Participants were randomly assigned to either the control or the experimental group. In addition to the standard of care, patients in the experimental group had access to genotypic drug-resistance testing, information used during treatment change and were followed up at six-and 12-months to determine virological suppression.</p></div><div><h3>Results</h3><p>A total of 261 patients with a median age of 32 (14.7–44.7) years were enrolled. In the intention-to-treat analysis, at 6-months, suppression was achieved in 58 (42.3%; 95% CI, 34.1–50.1) experimental group patients versus 51 (41.1%; 95% CI, 32.5–49.8) control group patients, with a p-value of 0.4. At-12 months, suppression was achieved in 110 (80.3%; 95% CI, 73.6–87) experimental patients versus 99 (79.8%; 95% CI, 72.8–86.9) control patients, with a <em>P</em>-value of 0.5. In the per-protocol analysis, at 6-months, suppression was observed in 38.46% (95% CI, 27.6–49.3) experimental patients versus 38.6% (95% CI, 26.0–51.2) control patients, with a <em>P</em>-value of 0.5. At 12-months, suppression was observed in 79.49% (95% CI, 70.5–88.5) of experimental patients versus 75.44% (95% CI, 64.3–86.6) of control patients, with a <em>P</em>-value of 0.3.</p></div><div><h3>Conclusion</h3><p>Conducting HIV drug-resistance testing, and switch to individualised second-line regimens did not significantly improve virological suppression in patients experiencing first-line ART failure in Tanzania.</p></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 73-81"},"PeriodicalIF":3.7,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524001498/pdfft?md5=05e7115e8de29c5cc7922dd58e6687e8&pid=1-s2.0-S2213716524001498-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142093502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shotgun metagenomic analysis reveals the emergence of plasmid-encoded mcr-5.1 gene in hospital wastewater in Bangladesh","authors":"Abu Sayem Khan ,&nbsp;Sunjida Afrin ,&nbsp;Firoz Ahmed ,&nbsp;Sabita Rezwana Rahman","doi":"10.1016/j.jgar.2024.08.007","DOIUrl":"10.1016/j.jgar.2024.08.007","url":null,"abstract":"<div><p>Colistin is considered the last line therapy for treating multidrug-resistant (MDR) bacterial infections in humans. Therefore, the spread of colistin resistance poses a serious threat to human, and environmental health. Though Bangladesh is known as a hotspot of AMR, limited studies have been carried out regarding the status of colistin resistance. Information on the emerging bacterial resistance is inevitable for protecting public health. Nowadays, wastewater analysis has been prioritized for metagenomics-enabled AMR surveillance. Our study on the metagenomic analysis of untreated hospital effluents first detected the colistin resistance-conferring <em>mcr-5.1</em> gene in the hospital environment of Bangladesh. Phylogenetic tree and <em>in silico</em> AMR analysis confirmed the detection of this <em>mcr-5</em> variant, which is located in a plasmid contig. The plasmid was untypeable and belonged to the bacteria from the Enterobacteriaceae family. The <em>mcr-5.1</em> operon was embedded in a Tn3 transposon, suggesting the mobility of the gene. <em>Tnshfr1</em> transposon, chromate resistance protein ChrB, DNA invertase hin, and two MFS-type proteins were present in the genetic environment of <em>mcr-5.1</em>. Our findings provide evidence of the occurrence of <em>mcr-5.1</em> in a hospital environment in Bangladesh, which calls for immediate attention and effective measures to contain the dissemination of colistin resistance in the environment.</p></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 22-26"},"PeriodicalIF":3.7,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524001589/pdfft?md5=7fca164298a25475fe1646d3e994f5b2&pid=1-s2.0-S2213716524001589-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142093500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}