Journal of global antimicrobial resistance最新文献

{"title":"Impact of COVID-19 on epidemiology and mortality risk factors in patients with carbapenem-resistant Acinetobacter baumannii bloodstream infections in a tertiary care hospital in Thailand","authors":"Darunee Chotiprasitsakul ,&nbsp;Korchart Ao-udomsuk ,&nbsp;Pitak Santanirand","doi":"10.1016/j.jgar.2025.04.015","DOIUrl":"10.1016/j.jgar.2025.04.015","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the epidemiology of <em>Acinetobacter baumannii</em> isolates and bloodstream infections (BSIs) during the COVID-19 pandemic.</div></div><div><h3>Methods</h3><div><em>A. baumannii</em> isolated from clinical specimens were identified between 2019 and 2023. The quarterly incidence of carbapenem-resistant <em>A. baumannii</em> (CRAB) was described. Hospital-wide carbapenem consumption was quantified as defined daily doses (DDD). Risk factors for CRAB acquisition and 30-day all-cause mortality in CRAB BSIs were determined.</div></div><div><h3>Results</h3><div>A total of 400 new CRAB cases were identified. The incidence of total CRAB clinical isolates fluctuated (<em>P</em> = 0.68), while the incidence of CRAB BSIs increased (<em>P</em> = 0.04). No correlation was found between CRAB incidence and hospital-wide carbapenem consumption (<em>P</em> = 0.93). There were 75 CRAB BSI cases and 47 non-carbapenem-resistant <em>A. baumannii</em> (NCRAB) BSI cases. Prior antibiotic exposure (aOR 8.67; <em>P</em> &lt; 0.001) and presence of multidrug-resistant organisms (MDROs) within 90 days (aOR 4.63; <em>P</em> = 0.02) were significantly associated with CRAB BSI acquisition. CRAB BSIs had higher 30-day mortality (66.67%) than NCRAB BSIs (25.53%) (aHR 0.83, 95% CI 0.26–2.59; <em>P</em> = 0.74). Pitt bacteremia score (aHR 1.20; <em>P</em> = 0.01) and recent COVID-19 infection (aHR 2.86; <em>P</em> = 0.03) were independent predictors of mortality in CRAB BSIs.</div></div><div><h3>Conclusions</h3><div>The incidence of total CRAB clinical isolates fluctuated, while the incidence of CRAB BSIs significantly increased. Previous antimicrobial exposure and presence of MDROs within 90 days were risk factors for CRAB. Pitt bacteremia score and recent COVID-19 infection were linked to higher CRAB BSI mortality.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 155-161"},"PeriodicalIF":3.7,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular typing and clinical characteristics of Clostridioides difficile infection in patients with inflammatory bowel disease: A retrospective study","authors":"Jiayiren Li ,&nbsp;Jing Yang ,&nbsp;Zirou Ouyang ,&nbsp;Minghui Ren ,&nbsp;Jianhong Zhao","doi":"10.1016/j.jgar.2025.04.011","DOIUrl":"10.1016/j.jgar.2025.04.011","url":null,"abstract":"<div><h3>Objectives</h3><div>The increasing incidence of <em>Clostridioides difficile</em> infection (CDI) has been associated with poorer prognosis of patients with inflammatory bowel disease (IBD). However, relevant data are limited in China. The aim of this study was to clarify the molecular types and clinical characteristics of <em>C. difficile</em> in IBD patients in China.</div></div><div><h3>Methods</h3><div>Stool samples were collected, cultured anaerobically, and tested for glutamate dehydrogenase and <em>C. difficile</em> toxins A and B. Toxigenic <em>C. difficile</em> isolates were subjected to multi-locus sequence typing and antimicrobial susceptibility testing. Clinical data were collected to compare IBD and non-IBD patients with CDI, and to determine the risk factors associated with CDI in IBD patients.</div></div><div><h3>Results</h3><div>The incidence of CDI was significantly higher in IBD patients than non-IBD patients (27.2% vs. 9.0%, respectively). Among IBD patients, the dominant sequence types (STs) were ST54 (20.2%), ST2 (14.9%), ST3 (14.9%), and ST42 (13.2%). The STs with the highest multidrug resistance rates were ST37 (100%), ST35 (100%), and ST42 (73.3%). In IBD patients, hospitalization within 6 months and use of 5-aminosalicylic acid were independent risk factors for CDI. Other risk factors included the use of proton pump inhibitors, immunosuppressants, and corticosteroids.</div></div><div><h3>Conclusion</h3><div>The incidence of CDI was significantly higher in IBD patients than non-IBD patients. Surveillance of CDI in hospitalized patients should be strengthened to reduce the incidence of CDI in IBD patients.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 188-197"},"PeriodicalIF":3.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of novel phages with antibiotic combinations against ESBL-producing and carbapenem-resistant Escherichia Coli","authors":"Md Shamsuzzaman ,&nbsp;Shukho Kim ,&nbsp;Jungmin Kim","doi":"10.1016/j.jgar.2025.04.005","DOIUrl":"10.1016/j.jgar.2025.04.005","url":null,"abstract":"<div><h3>Objectives</h3><div>The emergence of extended-spectrum <em>β</em>-lactamase (ESBL)-producing <em>E. coli</em> and carbapenem-resistant <em>E. coli</em> (CREC) is a significant global health challenge. This study focuses on isolating and characterizing two novel phages, EC.W1–9 and EC.W15–4, and investigating their efficacy with antibiotics against these resistant <em>E. coli</em><strong>.</strong></div></div><div><h3>Methods</h3><div><em>In vivo</em> experiments were conducted using BALB/c mice, and <em>E.coli</em> isolates were collected, cultured, and evaluated for antibiotic susceptibility. Phages were isolated from hospital sewage and prepared to infect the <em>E. coli</em>.</div></div><div><h3>Results</h3><div>The isolated phages, EC.W1–9 and EC.W15–4, belonged to the <em>Podoviridae</em> and <em>Straboviridae</em> families, and lack integrase or toxin-coding genes, indicating safety for therapeutic use. The combination of these phages significently enhanced their lytic ability, lysing 61.7% of 60 <em>E. coli</em> isolates, compared to 41.6%–55% lysis by individual phages. Furthermore, the phage combination demonstrated 100% susceptibility against different <em>E. coli</em> sequence types, including ST73, ST648, ST2311, ST405, ST7962, ST131, ST13003, and ST167. Additionally, synergy between antibiotics and phage combinations improved susceptibility rates to 73.3% for ESBL producers and 54% for CREC. The combined treatment of isolated phages and antibiotics significantly increased survival rates in BALB/c mice exposed to resistant STs of <em>E.coli</em>, including ST131, ST648, and ST410. Survival rates against ST131 increased by approximately 75% and 50% compared to treatment individual phages. Combined treatment with two phages and antibiotics resulted in 75–100% survival against <em>E. coli</em> ST410 and 100% survival against ST648</div></div><div><h3>Conclusions</h3><div>This study highlights the therapeutic importance of phage and phage-antibiotic combinations in combating ESBL-producing <em>E. coli</em> and CREC isolates.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 86-97"},"PeriodicalIF":3.7,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of NDM-1-producing Klebsiella pneumoniae ST11 clonal strains in Salvador, Brazil","authors":"Giulyana Evelyn Oliveira da Silva ,&nbsp;Adriano Souza Santos Monteiro ,&nbsp;Adriele Pinheiro Bomfim ,&nbsp;Helena Ferreira Leal ,&nbsp;Daniela da Silva Nascimento ,&nbsp;Humberto Fonseca de Freitas ,&nbsp;Verônica de França Diniz Rocha ,&nbsp;Márcio de Oliveira Silva ,&nbsp;Maria Goreth Barberino ,&nbsp;Edilane Lins Gouveia ,&nbsp;Mitermayer Galvão Reis ,&nbsp;Joice Neves Reis","doi":"10.1016/j.jgar.2025.04.008","DOIUrl":"10.1016/j.jgar.2025.04.008","url":null,"abstract":"","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 76-78"},"PeriodicalIF":3.7,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methicillin-resistant Staphylococcus aureus GG0398 on livestock farms and meat in the Netherlands","authors":"Engeline van Duijkeren ,&nbsp;Mike S.M. Brouwer ,&nbsp;Bart Wullings ,&nbsp;Michel Rapallini ,&nbsp;Ben Wit ,&nbsp;Tryntsje Cuperus ,&nbsp;Paul D. Hengeveld ,&nbsp;Sandra Witteveen ,&nbsp;Antoni P.A. Hendrickx ,&nbsp;Cindy M Dierikx ,&nbsp;Kees T. Veldman","doi":"10.1016/j.jgar.2025.04.007","DOIUrl":"10.1016/j.jgar.2025.04.007","url":null,"abstract":"<div><h3>Objectives</h3><div>The objective of this collaborative surveillance project was to investigate the prevalence of methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) among livestock farms, persons working/living on these farms, and on meat.</div></div><div><h3>Methods</h3><div>Samples from Dutch broiler, pig, veal calf, dairy cattle, and sheep farms, persons living/working on these farms and retail meat collected between 2018 and 2023 were cultured using (pre-) enrichment and selective plates. Next-generation sequencing of a subset of MRSA was performed to detect <em>mecA/mecC</em>, Panton-Valentine leucocidin (PVL) and to assign genogroups (GGs).</div></div><div><h3>Results</h3><div>On 113 of 149 (75.8%) pig farms, MRSA was present. The prevalence was lower among veal calf (44/173; 25.4%), and dairy (11/181; 6.1%), sheep (7/156; 4.5%), and broiler farms (0/195; 0%). Among 375 persons working/living on the farms, we identified 17 (4.5%) nasal MRSA carriers and all were farmers. All but two isolates from the farms belonged to GG0398 (= CC398). In total, 4529 Dutch retail meat samples were analysed and 412 (9.1%) were MRSA-positive. Poultry meat was most often contaminated. Most meat isolates (97/148; 65.5%) belonged to GG0398. All but one isolate carried <em>mecA,</em> and all were PVL-negative.</div></div><div><h3>Conclusions</h3><div>Despite the reduction of antimicrobial use by &gt; 70% in veterinary medicine since 2009, most pig farms are still MRSA positive. Farmers have a higher risk of being a nasal MRSA carrier than persons in the general population. Meat is regularly contaminated with MRSA, but this is considered a limited risk for consumers. Almost all the MRSA belonged to GG0398 confirming the continuous predominance of this type of MRSA in livestock and on meat.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 79-85"},"PeriodicalIF":3.7,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First identification and whole-genome characterization of human-associated Enterobacter roggenkampii and Enterobacter sichuanensis carrying blaIMI-4 carbapenemase in Costa Rica","authors":"Ricardo Gutiérrez ,&nbsp;Tábata Elizondo-Álvarez ,&nbsp;Manuel Ramírez-Cardoce ,&nbsp;Francisco Duarte-Martínez ,&nbsp;Estela Cordero-Laurent ,&nbsp;Elvira Segura-Retana ,&nbsp;Daniel Cascante-Serrano ,&nbsp;Romein Walters-Mais ,&nbsp;Roberto G. Melano ,&nbsp;María Antonieta Jiménez-Pearson","doi":"10.1016/j.jgar.2025.04.004","DOIUrl":"10.1016/j.jgar.2025.04.004","url":null,"abstract":"<div><h3>Objective</h3><div>All carbapenem-resistant Gram-negative bacterial isolates obtained in the Costa Rican National Network of Bacteriology Laboratories are routinely referred to the National Reference Center for Bacteriology for the national-laboratory for the surveillance and characterization of their carbapenem-related mechanism of resistance. As a result of this healthcare-associated infection surveillance, two bacterial isolates with unknown mechanisms for carbapenem resistance were investigated.</div></div><div><h3>Methods</h3><div>The isolates were subjected to taxonomic identification, antimicrobial susceptibility testing, and whole-genome characterization, using Illumina and Oxford Nanopore technologies, and bioinformatic analyses.</div></div><div><h3>Results</h3><div>The isolates were identified as <em>Enterobacter roggenkampii</em> and <em>Enterobacter sichuanensis</em>. Although the isolates presented different antimicrobial resistance profiles, both were resistant to carbapenems, positive for the modified carbapenem inactivation method (mCIM), and showed inhibition of carbapenem resistance with boronic acid. The full characterization of the isolates’ genomes, revealed the presence of the rare chromosomal <em>bla</em>_IMI-4 gene inserted within 25-kb integrative mobile elements, closely related to EcloIMEX-2.</div></div><div><h3>Conclusions</h3><div>The limited global distribution of the <em>bla</em>_IMI-4 variant, along with its identification in two distinct bacterial species in Costa Rica within integrative mobile elements, underscores the critical need for regular antibiotic resistance surveillance using next-generation sequencing technologies. This study demonstrates that this practice allows the timely detection of novel or rare mechanisms of antibiotic resistance.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 68-75"},"PeriodicalIF":3.7,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global and regional trends in Clostridioides difficile infection: An analysis from 1990 to 2021","authors":"Xiaoxia Wang ,&nbsp;Jing Pan ,&nbsp;Ting Fu ,&nbsp;Zhongshu Pu ,&nbsp;Kun Liu","doi":"10.1016/j.jgar.2025.04.001","DOIUrl":"10.1016/j.jgar.2025.04.001","url":null,"abstract":"<div><h3>Objectives</h3><div>The epidemiological situation of <em>Clostridioides difficile</em> infection (CDI) has dramatically changed over the past 30 years, but few studies have systematically analysed changes in the global epidemic landscape. This study aimed to delineate the global, regional, and national burden of CDI from 1990 to 2021.</div></div><div><h3>Methods</h3><div>CDI data were derived from the Global Burden of Disease Study 2021. Joinpoint regression analysis assessed changes over time and calculated annual percentage change (APC) statistics. An age-period-cohort model was adopted to estimate net/local drifts as well as the age, period, and cohort effects of CDI mortality. The relationship between the sociodemographic index (SDI) and antibiotic use and disease burden was analysed.</div></div><div><h3>Results</h3><div>The age-standardised death rate (ASDR) increased from 0.10 per 100,000 persons in 1990 to 0.19 per 100,000 persons in 2021, with an estimated annual percentage change of 2.76 (95% confidence interval [CI]: 2.18, 3.33). The ASDR of global CDI exhibited an upward trend from 1990 to 2021, with an AAPC of 2.26 (95% CI: 1.77, 2.76), but a downward trend from 2017 to 2021, with an APC of -2.04 (95% CI: -3.17, -0.90). Antibiotic use was significantly positively associated with CDI burden. The defined daily dose of all antibiotic use per 1000 inhabitants was positively correlated with ASDRs in high, high-middle, and low SDI regions (<em>P</em> <em>&lt;</em> 0.001, <em>P</em> &lt; 0.001, and <em>P</em> <em>&lt;</em> 0.001, respectively).</div></div><div><h3>Conclusions</h3><div>The burden of CDI has increased globally from 1990 to 2021, revealing significant spatial disparities. Antibiotic use is positively associated with CDI mortality.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 59-67"},"PeriodicalIF":3.7,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of low-frequency integrase strand transfer inhibitor resistance mutations on virological outcomes in antiretroviral therapy-naïve individuals initiating second-generation integrase inhibitors","authors":"Daniele Armenia ,&nbsp;Greta Marchegiani ,&nbsp;Daniele Spalletta ,&nbsp;Luca Carioti ,&nbsp;Alessandro Tavelli ,&nbsp;Maria Concetta Bellocchi ,&nbsp;Vincenzo Spagnuolo ,&nbsp;Valentina Mazzotta ,&nbsp;Eugenia Quiros-Roldan ,&nbsp;Valeria Bono ,&nbsp;Stefania Carrara ,&nbsp;Sergio Lo Caputo ,&nbsp;Antonella D’Arminio Monforte ,&nbsp;Francesca Ceccherini-Silberstein ,&nbsp;Stefano Rusconi ,&nbsp;Maria Mercedes Santoro ,&nbsp;the ICONA Study Group","doi":"10.1016/j.jgar.2025.04.002","DOIUrl":"10.1016/j.jgar.2025.04.002","url":null,"abstract":"<div><h3>Objectives</h3><div>This study investigated the role of low-frequency integrase strand transfer inhibitor (INSTI) resistance mutations, detectable by next-generation sequencing (NGS), at predicting virological rebound (VR) among people with HIV (PWH) starting second-generation INSTI-based first-line regimens.</div></div><div><h3>Methods</h3><div>This case-control study compared PWH (retrieved from the ICONA cohort; <span><span>www.icona.org</span><svg><path></path></svg></span>) who experienced VR (cases) with those who maintained virological control (controls) under first-line regimens based on dolutegravir or bictegravir. NGS data obtained through the Illumina platform were interpreted using the HIVdb algorithm version 9.7. Major (MRM), accessory (ARM), and other (ORM) INSTI resistance mutations were analysed at 5%, 10%, and 20% NGS cut-offs, respectively. Conditional logistic regression was used to evaluate the association between INSTI resistance and risk of VR.</div></div><div><h3>Results</h3><div>Among 266 PWH (90 cases, 176 controls), cases experienced VR with a median (interquartile range) viremia of 317 (93–6060) copies/mL after 15 (8–28) months from antiretroviral therapy start. The prevalence of MRM was low (NGS cut-off 5%, 10%, 20%: 1.9%, 0.8%, 0.4%, respectively), while it was moderate for ARM (7.5%, 7.1%, 6.4%) and high for ORM (50.0%, 44.7%, 42.1%). There was no evidence of a difference in prevalence of ≥1 MRM, ARM, or ORM between cases and controls. At 5% NGS cut-off, the prevalence of ≥2 ORM was higher in cases compared with controls. After adjusting for confounders, including HIV-1 subtype, ≥2 ORM detected as minority variants remained associated with VR risk.</div></div><div><h3>Conclusion</h3><div>Our findings suggest that combinations of low-frequency ORM may increase the risk of VR in individuals starting dolutegravir or bictegravir-based regimens. Further studies are needed to better understand these findings.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 51-58"},"PeriodicalIF":3.7,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome sequencing of non-aureus Staphylococcus species from poultry barn bioaerosols across Alberta reveals circulation of isolates with multiple antibiotic resistant genes and disinfectant resistant genes","authors":"Awais Ghaffar ,&nbsp;Karen Liljebjelke ,&nbsp;Sylvia Checkley ,&nbsp;Frank van der Meer ,&nbsp;Muhammad Farooq ,&nbsp;Heshanthi Herath Mudiyanselage ,&nbsp;Mohamed Faizal Abdul-Careem","doi":"10.1016/j.jgar.2025.03.014","DOIUrl":"10.1016/j.jgar.2025.03.014","url":null,"abstract":"<div><h3>Objectives</h3><div>Poultry environment has vast variety of bacterial species and non-<em>aureus Staphylococcus</em> species (NASS) are predominantly found. Various NASS are normal inhabitant of gastrointestinal tract and skin of chickens. The study objective was to isolate and identify NASS from bioaerosols of layer chicken barns across Alberta, leading to the phenotypic and genotypic antimicrobial resistance (AMR) profiling of the isolated bacterial colonies.</div></div><div><h3>Methods</h3><div>A total of 15 barns were sampled in this study with an XMX-CV microbial air sampler. Phenotypic AMR profiles were determined using the Sensititre® broth microdilution method on the standard CMV3AGPF plates and whole genome sequencing (WGS) was conducted to confirm the species and AMR genes.</div></div><div><h3>Results</h3><div>Seven <em>Staphylococcus equorum</em>, 5 <em>Staphylococcus shinii</em>, 1 <em>Staphylococcus pseudoxylosus</em>, 1 <em>Staphylococcus cohnii</em>, and 1 <em>Staphylococcus gallinarum</em> isolates were confirmed and identified by WGS and comparative genomic analysis. One of the <em>S. equorum</em> isolate was genetically highly divergent from the remaining isolates of the study. The phenotypic susceptibility profile showed 73.3% (11/15) of the isolates were resistant to lincomycin, 66.7% (10/15) were resistant to tetracycline, 33.3% (5/15) were resistant to streptomycin, 20% (3/15) were resistant to erythromycin, and 6.3% were resistant to gentamicin. A total of 10 antimicrobial resistance genes (ARGs) for antibiotics and 1 gene encoding resistance to disinfectants were detected among the study isolates by WGS.</div></div><div><h3>Conclusions</h3><div>Normal bioaerosol microflora having ARGs may lead to reduced therapeutic effectiveness of antibiotics in poultry and these ARGs can be a source of serious public health concern.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 18-26"},"PeriodicalIF":3.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of an F2:A-:B- epidemic plasmid harbouring tet(X4) in Escherichia coli isolated from a pig slaughterhouse in China","authors":"Xin Huang ,&nbsp;Xing-Xing Zhang ,&nbsp;Yue Jiang ,&nbsp;Peng-Yun Shao ,&nbsp;Qin-Chun Ma ,&nbsp;Xue-Qin Li ,&nbsp;Xinan Jiao ,&nbsp;Qiuchun Li ,&nbsp;Fa-Gang Zhong ,&nbsp;Jing Wang","doi":"10.1016/j.jgar.2025.03.013","DOIUrl":"10.1016/j.jgar.2025.03.013","url":null,"abstract":"","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"43 ","pages":"Pages 15-17"},"PeriodicalIF":3.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}