Journal of Feline Medicine and Surgery最新文献

{"title":"Osteochondrodysplasia and the c.1024G>T variant of <i>TRPV4</i> gene in Scottish Fold cats: genetic and radiographic evaluation.","authors":"Stefano Sartore, Riccardo Moretti, Lisa Adele Piras, Maurizio Longo, Stefania Chessa, Paola Sacchi","doi":"10.1177/1098612X231211763","DOIUrl":"10.1177/1098612X231211763","url":null,"abstract":"<p><strong>Objectives: </strong>The objectives of this study were to investigate the c.1024G>T SNP in the <i>TRPV4</i> gene in Scottish Straight and Fold cats, and to evaluate the pattern of skeletal phenotype and the evolution of radiological signs of Scottish Fold osteochondrodysplasia (SFOCD) over time in heterozygous subjects.</p><p><strong>Methods: </strong>DNA was obtained from blood samples of 17 cats (Scottish Fold: n = 12; Scottish Straight: n = 5) and subsequently genotyped by sequencing in a 249 bp region of the <i>TRPV4</i> gene (exon 6), including the known c.1024G>T causative mutation for osteochondrodysplasia. Orthopaedic and radiographic analyses were performed on animals carrying the mutant allele.</p><p><strong>Results: </strong>Genotyping by sequencing confirmed that all and only the Scottish Fold cats carried the mutant allele in a heterozygous asset. Furthermore, two other exon variants, already described in the literature as silent variants, were found in some of the sampled cats. Comparative orthogonal radiographic views of the shoulder, elbow, carpus, hip, stifle and tarsus were obtained. A mediolateral projection of the thoracic and lumbar column was also performed. Three out of four cats were clinically and radiographically examined again 1.5 years later.</p><p><strong>Conclusions and relevance: </strong>Although the presence of the mutant allele in all the tested Scottish Fold cats was confirmed, only 1/12 showed clinical signs of SFOCD. Furthermore, no cats in the 1.5-year follow-up showed skeletal changes. Although significant, the c.1024G>T mutation in the <i>TRPV4</i> gene, supposedly, is not the only cause or risk of developing SFOCD.</p>","PeriodicalId":15851,"journal":{"name":"Journal of Feline Medicine and Surgery","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10811760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138487730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recasting the gold standard - part II of II: communicating healthcare options along a continuum of care.","authors":"Ryane E Englar","doi":"10.1177/1098612X231215639","DOIUrl":"10.1177/1098612X231215639","url":null,"abstract":"<p><strong>Series outline: </strong>This is the second part of a two-part series on spectrum of care that encourages practitioners to tailor case management to the patient along a continuum of acceptable options. Part I defined the spectrum of care and broadened its approach beyond initial cost-of-care considerations. This second article introduces strategies for initiating conversations with clients about their needs, wants and expectations specific to healthcare options and case management decisions. It will explore how open inquiry, reflective listening, transparency and unconditional positive regard facilitate dialogue between providers and their clients as they collaborate on decision-making along a spectrum of care.</p><p><strong>Relevance: </strong>Contextual case management prioritizes interventions that are appropriate for both the patient and the client. To identify and explore which healthcare options represent the best fit for those impacted most by medical decisions, veterinarians need to make space for clients to feel comfortable sharing their perspectives. Clients are more likely to be engaged in decision-making when their insight is actively solicited. They have much to share with us as experts about their cat's overall health and wellness needs. Inviting clients to contribute to the consultation and being receptive to hearing what motivates their choices helps us to structure conversations around healthcare options. The ability to communicate healthcare options is as vital as the provision of healthcare, if not more so.</p>","PeriodicalId":15851,"journal":{"name":"Journal of Feline Medicine and Surgery","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10811761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138829995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence of feline idiopathic cystitis and urethral obstruction during COVID-19 human movement restrictions in Queensland, Australia.","authors":"Kayla Ann Jackson, Kate Eliza Collins, Te Yeun Kim, Rebekah E Donaldson","doi":"10.1177/1098612X231214931","DOIUrl":"10.1177/1098612X231214931","url":null,"abstract":"<p><strong>Objectives: </strong>Feline idiopathic cystitis (FIC) and urethral obstruction (UO) are commonly linked to increased stress. The influence of human movement restrictions on their incidence remains undetermined. FIC with or without UO is associated with environmental stress factors. The severe acute respiratory syndrome coronavirus 2 (COVID-19) pandemic restricted human movement and working behaviours. It is unknown if these restrictions increased the risk of FIC or UO in cats.</p><p><strong>Methods: </strong>Total cat emergency accessions and transfers between 8 February 2019 and 8 February 2021 at two private hospitals were retrospectively reviewed. Cats were included in the FIC group if they presented with lower urinary tract signs and supporting urinalysis, and were included in the UO group if they presented with UO. Cats with current urinary tract infection, or previous FIC or UO, were excluded. Groups were considered 'pre-COVID-19' between February 2019 and 2020 and 'COVID-19' between February 2020 and 2021. Cases of FIC and UO were compared between COVID-19 and pre-COVID-19 using Fisher's exact test and relative risk (RR) calculations.</p><p><strong>Results: </strong>The pre-COVID-19 incidence of FIC was 4.3% (63/1477, 95% confidence interval [CI] 0.0332-0.053), non-obstructive FIC was 1.4% (20/1477, 95% CI 0.008-0.020) and UO was 2.9% (43/1477, 95% CI 0.020-0.038). One cat was excluded as obstruction occurred during hospitalisation. The COVID-19 incidence of FIC was 5.4% (113/2081, 95% CI 0.044-0.64), non-obstructive FIC was 2.1% (70/2081, 95% CI 0.014-0.027) and UO was 3.4% (70/2081, 95% CI 0.026-0.042). The risk of non-obstructive FIC (<i>P</i> = 0.122; RR 0.652, 95% CI 0.387-1.096), UO (<i>P</i> = 0.382; RR 0.839, 95% CI 0.577-1.22) or either (<i>P</i> = 0.098; RR 0.773, 95% CI 0.572-1.044) was not significantly higher in the COVID-19 period than the pre-COVID-19 period.</p><p><strong>Conclusions and relevance: </strong>No clear association between COVID-19 movement restrictions and the incidence of UO or non-obstructive FIC was found within this retrospective population.</p>","PeriodicalId":15851,"journal":{"name":"Journal of Feline Medicine and Surgery","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10811764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138805173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abdominal ultrasonographic findings of cats with feline infectious peritonitis: an update.","authors":"Thiago R Müller, Dominique G Penninck, Cynthia Rl Webster, Francisco O Conrado","doi":"10.1177/1098612X231216000","DOIUrl":"10.1177/1098612X231216000","url":null,"abstract":"<p><strong>Objectives: </strong>The aim of this study was to describe the abdominal ultrasonographic findings in cats with confirmed or presumed feline infectious peritonitis (FIP).</p><p><strong>Methods: </strong>This was a retrospective study performed in an academic veterinary hospital. The diagnosis of FIP was reached on review of history, signalment, clinical presentation, complete blood count, biochemistry panel, peritoneal fluid analysis, cytology and/or histopathology results from abnormal organs, and/or molecular testing (immunohistochemical or FIP coronavirus [FCoV] RT-PCR). Cats with confirmed FIP by molecular testing or with a highly suspicious diagnosis of FIP were included. Abdominal ultrasound examination findings were reviewed.</p><p><strong>Results: </strong>In total, 25 cats were included. Common clinical signs/pathology findings included hyperglobulinemia (96%), anorexia/hyporexia (80%) and lethargy (56%). Abdominal ultrasound findings included effusion in 88% and lymphadenopathy in 80%. Hepatic changes were noted in 80%, the most common being hepatomegaly (58%) and a hypoechoic liver (48%). Intestinal changes were noted in 68% of cats, characterized by asymmetric wall thickening and/or loss of wall layering, with 52% being ileocecocolic junction and/or colonic in location. Splenic changes were present in 36% of cats, including splenomegaly, mottled parenchyma and hypoechoic nodules. Renal changes were present in 32%, encompassing a hypoechoic subcapsular rim and/or cortical nodules. Mesenteric and peritoneal abnormalities were seen in 28% and 16% of cats, respectively. Most cats (92%) had two or more locations of abdominal abnormalities on ultrasound.</p><p><strong>Conclusions and relevance: </strong>The present study documents a wider range and distribution of ultrasonographic lesions in cats with FIP than previously reported. The presence of effusion and lymph node, hepatic and/or gastrointestinal tract changes were the most common findings, and most of the cats had a combination of two or more abdominal abnormalities.</p>","PeriodicalId":15851,"journal":{"name":"Journal of Feline Medicine and Surgery","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10811767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138805170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors associated with house-soiling in Italian cats.","authors":"Barbara Padalino, Martina Zappaterra, Martina Felici, Claire Ricci-Bonot, Leonardo Nanni Costa, Katherine Houpt, Alessandra Tateo","doi":"10.1177/1098612X231202482","DOIUrl":"10.1177/1098612X231202482","url":null,"abstract":"<p><strong>Objectives: </strong>The aim of the present study was to identify factors associated with house-soiling in Italian cats.</p><p><strong>Methods: </strong>A cross-sectional online survey collected information on respondents' and cats' details and litter management, and whether the cat showed house-soiling. Univariable and multivariable regression models were performed using house-soiling (present/absent) and the type of house-soiling (ie, urinary, faecal, concurrent urinary and faecal) as dummy variables.</p><p><strong>Results: </strong>Data from 3106 cats were obtained. The number of dogs and other cats in the household, the cat's age, the number, type and location of the litter boxes, the type of litter, and the frequency of litter scooping and full replacement were retained in the final multivariable regression model for house-soiling (model <i>P</i> <0.001, Akaike information criterion [AIC] = 2454.30). Urinary tract diseases, the type and number of litter boxes and the number of dogs in the household were associated with urinary house-soiling (model <i>P</i> <0.001, AIC = 534.08), and gastroenteric/musculoskeletal diseases, number of litter boxes and litter box location were associated with faecal house-soiling (model <i>P</i> <0.001, AIC = 448.52). Healthy cats, the number of dogs in the household, the type of litter and litter full replacement frequency were retained in the final multivariable regression model for the concurrent expression of urinary and faecal house-soiling (model <i>P</i> <0.001, AIC = 411.47).</p><p><strong>Conclusions and relevance: </strong>Meeting cats' preferences for litter and litter box type, location, behavioural needs and strict litter hygienic conditions is recommended. Cat owners need to be educated to prevent and manage house-soiling in their cats.</p>","PeriodicalId":15851,"journal":{"name":"Journal of Feline Medicine and Surgery","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10812005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136397699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical management of feline chronic kidney disease in Portugal: a questionnaire-based study.","authors":"Tomás Rodrigues Magalhães, Ana Luísa Lourenço, Ronald Jan Corbee, Felisbina Luísa Queiroga","doi":"10.1177/1098612X231206125","DOIUrl":"10.1177/1098612X231206125","url":null,"abstract":"<p><strong>Objectives: </strong>The aim of the study was to characterise the clinical management of feline chronic kidney disease (CKD) by veterinary practitioners in Portugal.</p><p><strong>Methods: </strong>A questionnaire-based study was designed to be completed by all veterinarians who had diagnosed and treated at least one case of feline CKD in the previous year.</p><p><strong>Results: </strong>A total of 409 veterinary practitioners responded to the questionnaire, with approximately half of them diagnosing 2-5 cases of feline CKD per month (n = 219, 53.5%). Although a high proportion of these reported using the guidelines published by the International Renal Interest Society (n = 379, 92.7%), only 19.1% (n = 78) systematically performed systolic blood pressure (SBP) measurements in all of their patients. A renal diet was advised by almost all respondents (n = 406, 99.3%), but 36.9% (n = 150) of them considered that it represented less than 75% of the daily food intake for most of their patients. This dietary intervention was often prescribed regardless of stage (n = 298, 73.4%) and without a proper gradual diet transition. Appetite stimulants were frequently prescribed (n = 366, 89.5%), as well as a calcium channel blocker (n = 171, 41.8%) and an angiotensin-converting enzyme inhibitor (n = 245, 59.9%) to control systemic hypertension and proteinuria, respectively. Prescription of a phosphate binder was also common (n = 311, 76.0%). Regarding monitoring, 70.9% (n = 290) recommended that stable patients be reassessed every 2-3 months or more frequently, but only 35.7% (n = 146) were able to comply with this periodicity due to owners' constraints.</p><p><strong>Conclusions and relevance: </strong>The findings showed that although most survey respondents are aware of international guidelines for the clinical management of cats with CKD, the SBP measurement still needs to be more systematic to allow proper substaging and detection of systemic hypertension. The monitoring frequency was lower than recommended. Furthermore, the introduction of a renal therapeutic diet should be refined to improve its acceptance rate.</p>","PeriodicalId":15851,"journal":{"name":"Journal of Feline Medicine and Surgery","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10811999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138176373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical/research abstracts accepted for presentation at the 2023 ISFM Feline Congress","authors":"Melanie J Dobromylskyj, Agnieszka Zoltowska, Petra Černá, Samantha Evans, Séverine Tasker, D A Gunn-Moore, Nicole Jacque, Katie Clemens, Allison Koonce, Michael R Lappin, E. Spada, F. Bruno, G. Castelli, R. Perego, Fabrizio Vitale, A. Migliazzo, Sara Accettulli, L. Baggiani, D. Proverbio, Yan-Ru Choi, Regina Hofmann-Lehmann, O. Nekouei, V. Barrs, Julia A. Beatty, Maria Paz Iturriaga, Feline Congress, Simona Kovaříková, Izabela Firlova, Jana Bláhová","doi":"10.1177/1098612X231212124","DOIUrl":"https://doi.org/10.1177/1098612X231212124","url":null,"abstract":"Feline gastrointestinal eosinophilic sclerosing fibroplasia (FGESF) is a distinctive clinicopathological entity that primarily, but not exclusively, affects the gastrointestinal tract and associated lymph nodes. The condition is characterised by predominantly eosinophilic inflammation associated with a varied degree of fibroplasia, which together form a mass. Since the first reports more than 10 years ago, the number of cases has slowly increased, although FGESF is still considered a relatively uncommon differential for an abdominal mass. The aim of this study was to identify and characterise a large cohort of cases diagnosed as FGESF via biopsy. Cats with a diagnosis of FGESF based on histopathology samples submitted to a large commercial diagnostic laboratory between","PeriodicalId":15851,"journal":{"name":"Journal of Feline Medicine and Surgery","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139293876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surgical Management Of Feline Biliary Tract Disease: Decision-making and techniques.","authors":"Daniel Low, John Williams","doi":"10.1177/1098612X231206846","DOIUrl":"10.1177/1098612X231206846","url":null,"abstract":"<p><strong>Practical relevance: </strong>Diseases of the hepatobiliary system are not uncommon in first-opinion practice. The anatomy of the feline hepatobiliary tract differs from that of dogs and may predispose cats to inflammatory hepatobiliary disease. Case management can be challenging, and the prognosis is variable and dependent on the underlying disease process.</p><p><strong>Clinical challenges: </strong>Biliary tract disease in the cat has non-specific clinical signs that overlap with those of diseases of other body systems. Diagnostic tests of the hepatobiliary system are widely available, but interpretation of results can be challenging. Deciding on medical or surgical management can also be challenging; for example, various degrees of cholestasis may be seen with different diseases and disease severities, and will influence the decision to manage the patient medically or surgically. Surgery is indicated in cases of bile peritonitis, severe cholestasis, focal neoplasia or disease that is refractory to medical management. Intensive perioperative and postoperative management, with 24 h hospitalisation facilities, is essential.</p><p><strong>Audience: </strong>This review article is written for the small animal general/primary care practitioner, with an emphasis on decision-making in the surgical management of feline hepatobiliary disease.</p><p><strong>Evidence base: </strong>Evidence available in the published literature is reviewed and presented, and the inherent limitations are discussed.</p>","PeriodicalId":15851,"journal":{"name":"Journal of Feline Medicine and Surgery","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10812002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71482333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of age-specific reference intervals for selected serum and urinary biomarkers in elderly cats.","authors":"Femke Mortier, Robby van Leeuwenberg, Sylvie Daminet, Dominique Paepe","doi":"10.1177/1098612X231207492","DOIUrl":"10.1177/1098612X231207492","url":null,"abstract":"<p><strong>Objectives: </strong>Annual health screening is recommended in elderly cats to allow the early detection of conditions such as chronic kidney disease (CKD) and hyperthyroidism. Nevertheless, age-specific reference intervals (RIs) for renal and thyroid parameters in this population are lacking. The aim of this study was to determine age-specific RIs for selected serum and urine biomarkers related to CKD and hyperthyroidism, namely serum creatinine (sCr), symmetric dimethylarginine (SDMA), phosphate (P), total calcium (tCa), total thyroxine (TT4), urinary protein:creatinine ratio (UPC) and urine specific gravity (USG). These RIs were established for elderly cats (aged ⩾7 years) in general, as well as for mature adult cats (aged 7-10 years) and senior cats (aged >10 years) separately.</p><p><strong>Methods: </strong>A prospective study was conducted on client-owned cats aged ⩾7 years and considered healthy by their owners. The cats were screened to rule out metabolic and systemic diseases by means of a thorough history, complete physical examination, blood examination and urinalysis. The data from 206 healthy elderly cats (134 mature adult and 72 senior cats) were included. Age-appropriate RIs were determined following the guidelines of the American Society of Veterinary Clinical Pathology and compared with existing laboratory RIs.</p><p><strong>Results: </strong>Clinically relevant differences between the age-specific RI and the laboratory RI were found for several variables. Compared with the laboratory RI, the upper limit of the RI for cats aged ⩾7 years was lower for sCr, TT4 and P, and higher for SDMA. The lower limit of the age-appropriate RI was lower for USG. The new RI was almost identical to the existing laboratory RI for tCa and UPC.</p><p><strong>Conclusions and relevance: </strong>Using age-specific RIs for renal and thyroid biomarkers in mature adult and senior cats has important clinical consequences for the interpretation of health screening results in elderly cats. This confirms the need to adapt laboratory RIs to the specific animal population for which the RI will be used.</p>","PeriodicalId":15851,"journal":{"name":"Journal of Feline Medicine and Surgery","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10812001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138291160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyometra alters the redox status and expression of estrogen and progesterone receptors in the uterus of domestic cats.","authors":"Acácia Eduarda de Jesus Nascimento, Luciano Cardoso Santos, Bianca Reis Santos, Emilly Oliveira Santos, Maria Clara da Silva Galrão Cunha, Paola Pereira das Neves Snoeck, Mário Sérgio Lima de Lavor, Juneo Freitas Silva","doi":"10.1177/1098612X231170159","DOIUrl":"10.1177/1098612X231170159","url":null,"abstract":"<p><strong>Objectives: </strong>The aim of this study was to evaluate the expression profile of sex steroid receptors and redox mediators in the uterus of domestic cats with pyometra.</p><p><strong>Methods: </strong>Twelve cats were used and divided into groups: (1) non-gestational healthy diestrus (n = 7) and (2) pyometra (n = 5). The plasma profiles of estradiol and progesterone (P₄) as well as uterine expression levels of estradiol alpha (ERα), progesterone (PR) and androgen (AR) receptors, of the antioxidant enzymes superoxide dismutase 1 (SOD1), catalase and glutathione peroxidase 1 (GPX1), and of the oxidative damage marker 8-hydroxy-2'-deoxyguanosine (8-OHdG) were evaluated.</p><p><strong>Results: </strong>Cats with pyometra showed higher plasma P₄ levels and increased uterine messenger RNA (mRNA) and protein expression of ERα and PR, mainly in the glandular epithelium for ERα and in stromal and myometrial cells for PR. In addition, there was an increase in 8-OHdG immunostaining and GPX1 mRNA and protein expression in cats with pyometra compared with those in non-gestational diestrus, while catalase showed a reduction in endometrial immunostaining in cats with pyometra. There were no differences in uterine AR and SOD1 expression between the groups.</p><p><strong>Conclusion and relevance: </strong>The findings of this study showed that pyometra is associated with oxidative stress in the uterus of domestic cats and alterations of the profile of sex steroid receptors, especially ERα and PR, and of antioxidant enzymes, suggesting that changes in these mediators may play a role with the etiopathogenesis of this disease.</p>","PeriodicalId":15851,"journal":{"name":"Journal of Feline Medicine and Surgery","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10812004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138451651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}