Journal of Faculty Pharmacy of Istanbul University最新文献_第7页

CAPILLARY ELECTROPHORESIS METHOD FOR THE DETERMINATION OF LACTULOSE AND MANNITOL RATIO FOR ESTIMATING INTESTINAL PERMEABILITY 毛细管电泳法测定乳果糖与甘露醇比值估算肠通透性

Journal of Faculty Pharmacy of Istanbul University Pub Date : 2012-01-01 DOI: 10.16883/JFPIU.58224

E. Acar, D. Ö. Ünal, M. Acar, M. Koc

{"title":"CAPILLARY ELECTROPHORESIS METHOD FOR THE DETERMINATION OF LACTULOSE AND MANNITOL RATIO FOR ESTIMATING INTESTINAL PERMEABILITY","authors":"E. Acar, D. Ö. Ünal, M. Acar, M. Koc","doi":"10.16883/JFPIU.58224","DOIUrl":"https://doi.org/10.16883/JFPIU.58224","url":null,"abstract":"SUMMARY In this study, a simple, rapid and accurate capillary electrophoresis method has been developed for the determination of lactulose and mannitol in urine for estimating the intestinal permeability. The urine samples were prepared to analyze by using solid phase extraction. For analysis a background electrolyte (BGE) consisted of 6 mmol/L sorbic acid, 1.25mmol/L hexadecyltrimethylammoniumbromide (HDTMA), 50 mmol/L LiOH (pH 12.5) was used. Separation was performed at -5 kV over 20 min within the fused capillary column (45 cm, 50 I¼m I.D.) at 20 °C. The method developed was applied a series of urine sample to measure the lactulose/mannitol concentration rate. Key words: Lactulose, Mannitol, Determination, Permeability, Capillary electrophoresis OZET Bu calismada intestinal gecirgenligin incelenmesi amaciyla idrarda laktuloz ve mannitol tayini icin basit, hizli ve dogru bir kapiler elektroforez yontemi gelistirilmistir. Idrar ornekleri kati faz ozutleme yontemi ile analize hazirlanmistir. Analizler icin 6 mmol/L sorbik asit, 1.25 mmol/L hekzadesil trimetil amonyum bromur (HDTMA), 50 mmol/ L LiOH iceren pH si 12.5 e karsilik gelen destek elektrolit kullanilmistir. Ayrim 45 cm 50 I¼m ic capli fused silika kolonda 20 °C sicaklikta -5 kV ayirma potansiyeli kullanilarak 20 dk icerisinde gerceklestirilmistir. Gelistirilen yontem laktuloz / mannitol derisim oranlarini olcmek icin bir seri idrar numunesine uygulanmistir. Anahtar Kelimeler: Laktuloz, Mannitol, Tayin, Gecirgenlik, Kapiler elektroforez","PeriodicalId":15850,"journal":{"name":"Journal of Faculty Pharmacy of Istanbul University","volume":"343 1","pages":"13-22"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76399326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CYTOTOXIC EFFECTS OF TRIAZOLE FUNGUCIDES 三唑类杀菌剂的细胞毒作用

Journal of Faculty Pharmacy of Istanbul University Pub Date : 2012-01-01 DOI: 10.16883/JFPIU.12230

E. Bülbül, G. Bülbül, G. Özhan

{"title":"CYTOTOXIC EFFECTS OF TRIAZOLE FUNGUCIDES","authors":"E. Bülbül, G. Bülbül, G. Özhan","doi":"10.16883/JFPIU.12230","DOIUrl":"https://doi.org/10.16883/JFPIU.12230","url":null,"abstract":"SUMMARY Fungicides are at high risk exposure compounds both pre and post harvest time due to their usage during storage. Fungucides have various benefits on agricultural struggle. Apart from that, studies showed that fungucides also have mutagenic, carcinogenic, chronic toxic effects on nervous system, immune system, hormonal and reproductive system. Triazole group fungicides, prochloraz, tebuconazole, triadimenole and triadimefone, are commonly used in the world. In the study, it is aimed to emphasize about their importance on human and environmental health. And, we evaluated their potential cytotoxic effect on HeLa cells by using MTT and LDH tests. Before the tests application, the parameters like cell counts, incubation times, pesticide concentrations and test circumstances were examined for test optimization. As a result, for MTT cytotoxicity test IC50 values of prochloraz, tebuconazole, triadimefone and triadimenole were recorded in order as 0.4, >1, 0.24 and 0.37 mg/ml. As well for LDH test these values were identified in order as 0.065, 0.985, 0.052 and 0.042 mg/ml. MTT test, used to identify hazards on mitochondrial activity and respiratory chain, showed that cytotoxic effect of prochloraz, triadimefone and triadimenole was dose dependently increased. While tebuconazole showed cytotoxic effect on concentration range studied, it was seen that IC50 values for LDH test which is used to identify membrane damage were lower than IC50 values compared with MTT test. The obtained results would draw attention to expand researches on toxic effects of triazole fungicides which are identified as cytotoxic for human and environment health. Also, it is believed to take necessary precautions for usage of these compounds. Key words: Triazole fungicides, MTT test, LDH test, Cytotoxicity","PeriodicalId":15850,"journal":{"name":"Journal of Faculty Pharmacy of Istanbul University","volume":"18 1","pages":"23-31"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73575035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

THE WILD EDIBLE PLANTS OF WESTERN NIGDE ALADAGLAR MOUNTAINS (CENTRAL TURKEY) 阿拉达格勒山脉西部的野生可食植物(土耳其中部)

Journal of Faculty Pharmacy of Istanbul University Pub Date : 2011-01-01 DOI: 10.16883/JFPIU.16673

E. Ozdemir, .. K.Alpinar

引用次数: 6

PLANTS USED FOR MEDICINAL PURPOSES IN KARAMAN PROVINCE (SOUTHERN TURKEY) 卡拉曼省药用植物(土耳其南部)

Journal of Faculty Pharmacy of Istanbul University Pub Date : 2011-01-01 DOI: 10.16883/JFPIU.00477

N. Özhatay, .. S.Koçak

引用次数: 10

DETERMINATION OF BORONS IN 18 BOTTLED MINERAL WATERS IN TURKEY 土耳其18种瓶装矿泉水中硼的测定

Journal of Faculty Pharmacy of Istanbul University Pub Date : 2011-01-01 DOI: 10.16883/JFPIU.90732

P. Köseoğlu, .. U.Kolak

引用次数: 0

IN VITRO ANTIMICROBIAL ACTIVITIES OF WIDELY USED CHEMICAL DISINFECTANTS AGAINST VARIOUS MICROORGANISMS 常用化学消毒剂对多种微生物的体外抑菌活性研究

Journal of Faculty Pharmacy of Istanbul University Pub Date : 2011-01-01 DOI: 10.16883/JFPIU.15273

S. Döşler, A. Güzel, B. Çelik

引用次数: 0

HPLC-UV METHOD TRANSFER FOR UPLC IN BIO ANALYTICAL ANALYSIS: DETERMINATION OF TERBINAFINE FROM HUMAN PLASMA 高效液相色谱-紫外转移法用于生物分析分析:人血浆中特比萘芬的测定

Journal of Faculty Pharmacy of Istanbul University Pub Date : 2011-01-01 DOI: 10.16883/JFPIU.33235

.. D.Ozerunal

{"title":"HPLC-UV METHOD TRANSFER FOR UPLC IN BIO ANALYTICAL ANALYSIS: DETERMINATION OF TERBINAFINE FROM HUMAN PLASMA","authors":".. D.Ozerunal","doi":"10.16883/JFPIU.33235","DOIUrl":"https://doi.org/10.16883/JFPIU.33235","url":null,"abstract":"Analiz laboratuarlari yuksek miktardaki ornekleri en kisa zamanda analiz etmek zorundadir. Bu problemi cozmek icin hizli ve etkin yeni kalitatif ve kantitatif analiz yontemleri gelistirilmelidir. Ilac ve ilgili bilesikler icin sivi kromatografisi yontemi uygundur. Ancak, Yuksek Basincli Sivi Kromatografisi (YBSK) ile analiz sureleri 10-45 dakika arasindadir. Ultra Performans Sivi Kromatografisi (UPSK) kullanimi hizi ve hassasiyeti yuzunden yenilikci bir yaklasim olabilir. Farmasotik ve biomedikal analizlerde bu teknik son yillarda oldukca genis kullanim alam bulmustur. YBSK yontemlerinin UPSK sistemlerine aktarimi ilac endustrisinde ilac gelistirme ve kalite kontrol prosesinde zamani kisaltmak amaciyla sikca kullanilmaktadn. Terbmafniin insan plasmasindan analizi model olarak alinarak, gelistirilen hizli ve guvenilir YBSK yontemi UPSK'ne metod transferi yapilmistir. Ilk analiz yonteminde Tebinafinin plasmadan analizi HPLC sisteminde Zorbax SB-Aq C18 kolon kullanilmistir. Mobil faz, 50 % H 3 P0 4 cozeltisi/ Asetonitril (40/60) v/v), 0.8 ml/dak akis hizmda calisilmista. Terbinafin ve propil parabenin alikonma zamanlari sirasiyla, 17.43 ve 7.27 dakika bulunmustur. Gelistirilen bu metod benzer kolon dolgu materyali kullanilarak UPSK sistemine transfer edilmistir. Waters Acquity BEH C18 1.7 fim 2.1 x 50 mm kolon 50 % H 3 P0 4 cozeltisi/ACN (50/50, v/v) mobil faz olarak 0.3 ml/dakika akis hizinda kullanilmistir. Terbinafin ve propil parabenin alikonma zamanlan sirasiyla, 2.98 ve 0.92 dakika elde edilmistir. Terbinafinin UBSK ile analiz zamani UBSK ne oranla 6 kat daha azdir ve benzer pik ozelliklerinde, 2.98 dakika da elue edilmistir.","PeriodicalId":15850,"journal":{"name":"Journal of Faculty Pharmacy of Istanbul University","volume":"89 1","pages":"56-65"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80697805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

THE USE OF WILD EDIBLE PLANTS IN KURECIK (AKCADAG/MALATYA) 库列克(akcadag / malatya)野生食用植物的利用

Journal of Faculty Pharmacy of Istanbul University Pub Date : 2011-01-01 DOI: 10.16883/JFPIU.30064

Y. Yeşil, E. Akalın

引用次数: 13

TAXONOMICAL ANATOMICAL AND KARYOLOGICAL REMARKS ON TWO ENDEMIC CENTAUREA L. SPECIES IN TURKEY: C. kilaea Boiss. & C. hermannii F. Hermann 土耳其特有的两种半马属植物的分类、解剖和核学研究。赫尔曼

Journal of Faculty Pharmacy of Istanbul University Pub Date : 2011-01-01 DOI: 10.16883/JFPIU.45236

B. Gürdal, .. N.Özhatay

引用次数: 2

MORPHOLOGICAL AND ANATOMICAL CHARACTERISTICS OF Veronica turrilliana Stoj.& Stef. (Scrophulariaceae): THE RARE AND THREATENED SPECIES OF TURKEY 牛头草的形态解剖特征。&孙燕姿。(玄参科):土耳其的珍稀濒危物种

Journal of Faculty Pharmacy of Istanbul University Pub Date : 2011-01-01 DOI: 10.16883/JFPIU.04436

S. Demirci, N. Özhatay, E. Akalın

引用次数: 0