CAPILLARY ELECTROPHORESIS METHOD FOR THE DETERMINATION OF LACTULOSE AND MANNITOL RATIO FOR ESTIMATING INTESTINAL PERMEABILITY

Abstract

SUMMARY In this study, a simple, rapid and accurate capillary electrophoresis method has been developed for the determination of lactulose and mannitol in urine for estimating the intestinal permeability. The urine samples were prepared to analyze by using solid phase extraction. For analysis a background electrolyte (BGE) consisted of 6 mmol/L sorbic acid, 1.25mmol/L hexadecyltrimethylammoniumbromide (HDTMA), 50 mmol/L LiOH (pH 12.5) was used. Separation was performed at -5 kV over 20 min within the fused capillary column (45 cm, 50 I¼m I.D.) at 20 °C. The method developed was applied a series of urine sample to measure the lactulose/mannitol concentration rate. Key words: Lactulose, Mannitol, Determination, Permeability, Capillary electrophoresis OZET Bu calismada intestinal gecirgenligin incelenmesi amaciyla idrarda laktuloz ve mannitol tayini icin basit, hizli ve dogru bir kapiler elektroforez yontemi gelistirilmistir. Idrar ornekleri kati faz ozutleme yontemi ile analize hazirlanmistir. Analizler icin 6 mmol/L sorbik asit, 1.25 mmol/L hekzadesil trimetil amonyum bromur (HDTMA), 50 mmol/ L LiOH iceren pH si 12.5 e karsilik gelen destek elektrolit kullanilmistir. Ayrim 45 cm 50 I¼m ic capli fused silika kolonda 20 °C sicaklikta -5 kV ayirma potansiyeli kullanilarak 20 dk icerisinde gerceklestirilmistir. Gelistirilen yontem laktuloz / mannitol derisim oranlarini olcmek icin bir seri idrar numunesine uygulanmistir. Anahtar Kelimeler: Laktuloz, Mannitol, Tayin, Gecirgenlik, Kapiler elektroforez