Journal of fish diseases最新文献_第7页

Plasma and tissue transferrin and ferritin, and gene expression of ferritin, transferrin, and transferrin receptors I and II in channel catfish Ictalurus punctatus fed diets with different concentrations of inorganic or organic iron 以不同浓度的无机铁或有机铁为饵料的河豚血浆和组织中的转铁蛋白和铁蛋白，以及铁蛋白、转铁蛋白、转铁蛋白受体 I 和 II 的基因表达

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2024-04-14 DOI: 10.1111/jfd.13953

Isaac Buyinza, Grace Ramena, Rebecca Lochmann, Amit Sinha, Michele Jones

{"title":"Plasma and tissue transferrin and ferritin, and gene expression of ferritin, transferrin, and transferrin receptors I and II in channel catfish Ictalurus punctatus fed diets with different concentrations of inorganic or organic iron","authors":"Isaac Buyinza, Grace Ramena, Rebecca Lochmann, Amit Sinha, Michele Jones","doi":"10.1111/jfd.13953","DOIUrl":"10.1111/jfd.13953","url":null,"abstract":"Ferritin, transferrin, and transferrin receptors I and II play a vital role in iron metabolism, health, and indication of iron deficiency anaemia in fish. To evaluate the use of high-iron diets to prevent or reverse channel catfish (Ictalurus punctatus) anaemia of unknown causes, we investigated the expression of these iron-regulatory genes and proteins in channel catfish fed plant-based diets. Catfish fingerlings were fed five diets supplemented with 0 (basal), 125, and 250 mg/kg of either inorganic iron or organic iron for 2 weeks. Ferritin, transferrin, and transferrin receptor I and II mRNA and protein expression levels in fish tissues (liver, intestine, trunk kidney, and head kidney) and plasma were determined. Transferrin (iron transporter) and TfR (I and II) genes were generally highly expressed in fish fed the basal diet compared to those fed the iron-supplemented diets. In contrast, ferritin (iron storage) genes were more expressed in the trunk kidney of fish fed the iron-supplemented diets than in those fed the basal diet. Our results demonstrate that supplementing channel catfish plant-based diets with iron from either organic or inorganic iron sources affected the expression of the iron-regulatory genes and increased body iron status in the fish.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140579462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intestinal strangulation in farmed Atlantic cod (Gadus morhua): Pathological changes and possible predisposing anatomical features 养殖大西洋鳕鱼（Gadus morhua）的肠道绞伤：病理变化和可能的致病解剖特征

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2024-04-08 DOI: 10.1111/jfd.13955

Fredrik Strebel Skedsmo, Guro Løkka, Elvis Chikwati, Julianne Valla Jacobsen, Arild Espenes, Trond M. Kortner

引用次数: 0

Rainbow trout (Oncorhynchus mykiss, Walbaum 1792) adenocarcinoma investigation with various diagnostic imaging techniques 利用各种诊断成像技术调查虹鳟（Oncorhynchus mykiss, Walbaum 1792）腺癌

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2024-04-08 DOI: 10.1111/jfd.13951

Márton Hoitsy, György Hoitsy, János Gál, Árisz Ziszisz, Tamás Tóth, Endre Sós, Viktória Sós-Koroknai, Csaba Jakab, Örs Petneházy, Tamás Donkó, Tamás Molnár, Miklós Marosán

{"title":"Rainbow trout (Oncorhynchus mykiss, Walbaum 1792) adenocarcinoma investigation with various diagnostic imaging techniques","authors":"Márton Hoitsy, György Hoitsy, János Gál, Árisz Ziszisz, Tamás Tóth, Endre Sós, Viktória Sós-Koroknai, Csaba Jakab, Örs Petneházy, Tamás Donkó, Tamás Molnár, Miklós Marosán","doi":"10.1111/jfd.13951","DOIUrl":"10.1111/jfd.13951","url":null,"abstract":"Diagnostic imaging techniques provide a new aspect of the ante-mortem and post-mortem diagnostics in fish medicine. Ultrasonography, computed tomography (CT) and magnetic resonance imaging (MRI) can provide more information about the internal organs and pathognomic lesions. The authors used diagnostic imaging techniques to evaluate and describe the neoplastic malformation in a 3-year-old female rainbow trout (Oncorhynchus mykiss). The fish was examined with Siemens Somatom Definition AS + CT scanner and Siemens Biograph mMR scanner. The animal was lethargic and showed anorectic signs and muscular dystrophy. During the post-mortem investigation, histopathology and immunohistochemistry were also performed allowing us to identify the neoplasms. The results showed a large soft tissue mass in the first mid-intestine segment, which proved to be an adenocarcinoma. This subsequently led to digestion problems and absorption disorders. Immunohistochemically, neoplastic cells of carcinoma revealed E-cadherin and pancytokeratin positivity. This is the first study to report the use of MRI and CT for studying gastrointestinal adenocarcinoma in rainbow trout.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfd.13951","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140579353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outbreak of trypanosomiasis in cage‐cultured large yellow croaker in China 中国笼养大黄鱼爆发锥虫病

IF 2.5 3区农林科学

Journal of fish diseases Pub Date : 2024-04-03 DOI: 10.1111/jfd.13952

Pan Qin, Xingfu Chen, Bilian Lou, Tong Wu, Jingsong Yang, Weicheng Wang, Guangliang Huang, Xinhua Chen

引用次数: 0

Hepatic transcriptome profiling of largemouth bass (Micropterus salmoides Lacépède) injected with Flavobacterium covae or lipopolysaccharide. 大口鲈鱼（Micropterus salmoides Lacépède）肝脏转录组分析：注射黄杆菌或脂多糖。

IF 2.5 3区农林科学

Journal of fish diseases Pub Date : 2024-04-01 DOI: 10.1111/jfd.13948

Nilima N Renukdas, Anita M Kelly, Gaurav Zinta, Amit Kumar Sinha

{"title":"Hepatic transcriptome profiling of largemouth bass (Micropterus salmoides Lacépède) injected with Flavobacterium covae or lipopolysaccharide.","authors":"Nilima N Renukdas, Anita M Kelly, Gaurav Zinta, Amit Kumar Sinha","doi":"10.1111/jfd.13948","DOIUrl":"https://doi.org/10.1111/jfd.13948","url":null,"abstract":"Flavobacterium covae (columnaris) is the most detrimental bacterial disease affecting the largemouth bass (Micropterus salmoides Lacépède) aquaculture industry. In the current study, fish received an intraperitoneal injection of either 1× PBS (100 μL), LPS in PBS (100 μL, 10 μg/mL), or F. covae (100 μL, 2.85 × 1011 CFU/mL) to simulate immunological challenges. After 24 h post-injection, liver tissue from the control and treated groups were then collected for transcriptome analysis. Results of the Gene Ontology (GO) and KEGG pathway analyses for the F. covae and LPS-injected groups found differentially expressed genes (DEGs) enriched primarily in toll-like receptors (TLRs), cytokine-cytokine receptors, complement and coagulation cascades, and the PPAR signalling pathways. This suggests that the liver immune system is enhanced by these five combined pathways. Additionally, the DEGs TLR5, MYD88, and IL-1 were significantly upregulated in F. covae and LPS-injected fish compared to the controls, whereas IL-8 was downregulated. The upregulation of TLR5 was unexpected as F. covae lacks flagellin, the protein that binds to TLR5. Additionally, it is unknown whether the TLR5 is upregulated by LPS. Further research into the upregulation of TLR5 is warranted. These results provide insight into immune responses and associated pathways contributing to the immune system in the liver during columnaris infection and induced response to LPS in largemouth bass.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative transcriptome analysis explored the molecular mechanisms of a luxR-type regulator regulating intracellular survival of Aeromonas hydrophila 比较转录组分析探索了调控嗜水气单胞菌胞内存活的luxR型调控因子的分子机制。

IF 2.5 3区农林科学

Journal of fish diseases Pub Date : 2024-03-31 DOI: 10.1111/jfd.13949

Weiqin Chen, Leilei Mao, Qingpi Yan, Lingmin Zhao, Lixing Huang, Jiaonan Zhang, Yingxue Qin

{"title":"Comparative transcriptome analysis explored the molecular mechanisms of a luxR-type regulator regulating intracellular survival of Aeromonas hydrophila","authors":"Weiqin Chen, Leilei Mao, Qingpi Yan, Lingmin Zhao, Lixing Huang, Jiaonan Zhang, Yingxue Qin","doi":"10.1111/jfd.13949","DOIUrl":"10.1111/jfd.13949","url":null,"abstract":"Aeromonas hydrophila is not a traditional intracellular bacterium. However, previous studies revealed that pathogenic A. hydrophila B11 could temporarily survive for at least 24 h in fish phagocytes, and the regulation of intracellular survival in bacteria was associated with regulators of the LuxR-type. The mechanisms of luxR08110 on the A. hydrophila's survival in macrophages were investigated using comprehensive transcriptome analysis and biological phenotype analysis in this study. The results showed that after luxR08110 was silenced, the intracellular survival ability of bacteria was significantly diminished. Comparative transcriptome analysis revealed that luxR08110 was a critical regulator of A. hydrophila, which regulated the expression of over 1200 genes, involving in bacterial flagellar assembly and chemotaxis, ribosome, sulphur metabolism, glycerolipid metabolism, and other mechanisms. Further studies confirmed that after the inhibition of expression of luxR08110, the motility, chemotaxis and adhesion of A. hydrophila significantly decreased. Moreover, compared with the wild-type strain, the survival rates of silencing strain were all considerably reduced under both H2O2 and low pH stress conditions. According to both transcriptome analysis and phenotypic tests, the luxR08110 of A. hydrophila could act as global regulator in bacteria intracellular survival. This regulator regulated intracellular survival of A. hydrophila mainly through two ways. One way is to regulate bacterial flagellar synthesis and further affects the motility, chemotaxis and adhesion of bacteria. The other way is to regulate sulphur and glycerolipid metabolisms, thus affecting bacterial energy production and the ability to resist environmental stress.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140329812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression profiles of heat shock protein 70 and high-mobility groups box-1 protein in gnotobiotic brine shrimp challenged with different virulence levels of isogenic Vibrio harveyi strains 不同毒力水平的同源哈维氏弧菌菌株对非生物饲养的盐水虾中热休克蛋白 70 和高移动组 box-1 蛋白的表达谱。

IF 2.2 3区农林科学

Journal of fish diseases Pub Date : 2024-03-31 DOI: 10.1111/jfd.13954

Nguyen Thi Xuan Hong, Kartik Baruah, Hung Nguyen Van, Daisy Vanrompay, Peter Bossier

引用次数: 0

Most of the escaped farmed salmon entering a river during a 5-year period were infected with one or more viruses 在 5 年时间里，进入河流的大部分逃逸养殖鲑鱼都感染了一种或多种病毒。

IF 2.5 3区农林科学

Journal of fish diseases Pub Date : 2024-03-31 DOI: 10.1111/jfd.13950

Abdullah S. Madhun, Egil Karlsbakk, Øystein Skaala, Monica F. Solberg, Vidar Wennevik, Alison Harvey, Sonnich Meier, Per T. Fjeldheim, Kaja Christine Andersen, Kevin A. Glover

{"title":"Most of the escaped farmed salmon entering a river during a 5-year period were infected with one or more viruses","authors":"Abdullah S. Madhun, Egil Karlsbakk, Øystein Skaala, Monica F. Solberg, Vidar Wennevik, Alison Harvey, Sonnich Meier, Per T. Fjeldheim, Kaja Christine Andersen, Kevin A. Glover","doi":"10.1111/jfd.13950","DOIUrl":"10.1111/jfd.13950","url":null,"abstract":"Disease interactions between farmed and wild populations have been poorly documented for most aquaculture species, in part due to the complexities to study this. Here, we tested 567 farmed Atlantic salmon escapees, captured in a Norwegian river during 2014–2018, for five viral infections that are prevalent in global salmonid aquaculture. Over 90% of the escapees were infected with one or more viruses. Overall prevalences were: 75.7% for piscine orthoreovirus (PRV-1), 43.6% for salmonid alphavirus (SAV), 31.2% for piscine myocarditis virus (PMCV), 1.2% for infectious pancreatic necrosis virus (IPNV) and 0.4% for salmon anaemia virus (ISAV). A significantly higher prevalence of PMCV infection was observed in immature compared to mature individuals. The prevalence of both SAV and PMCV infections was higher in fish determined by fatty acid profiling to be ‘recent’ as opposed to ‘early’ escapees that had been in the wild for a longer period of time. This is the first study to establish a time-series of viral infection status of escapees entering a river with a native salmon population. Our results demonstrate that farmed escapees represent a continuous source of infectious agents which could potentially be transmitted to wild fish populations.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfd.13950","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140329814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative proteome analysis revealed potential biomarkers and the underlying immune mechanisms in Vibrio-resistant hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ 比较蛋白质组分析揭示了抗振动杂交石斑鱼♀×石斑鱼♂的潜在生物标志物及其免疫机制。

IF 2.5 3区农林科学

Journal of fish diseases Pub Date : 2024-03-24 DOI: 10.1111/jfd.13940

Nurhikmah Abu Aziz, Annie Christianus, Wan Mohd Syazwan Wan Solahudin, Intan Safinar Ismail, Chen-Fei Low

{"title":"Comparative proteome analysis revealed potential biomarkers and the underlying immune mechanisms in Vibrio-resistant hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂","authors":"Nurhikmah Abu Aziz, Annie Christianus, Wan Mohd Syazwan Wan Solahudin, Intan Safinar Ismail, Chen-Fei Low","doi":"10.1111/jfd.13940","DOIUrl":"10.1111/jfd.13940","url":null,"abstract":"Vibrio alginolyticus is the causative agent of vibriosis, a common bacterial infection in grouper aquaculture that is associated with the development of haemorrhagic and non-haemorrhagic ulcerations on the fish. In the present study, comparative proteome analysis was performed on serum samples from Vibrio-resistant and Vibrio-susceptible grouper. Samples were analysed using high-throughput LC-MS/MS and identified 2770 unique peptides that corresponded to 344 proteins. Subsequent analysis identified 21 proteins that were significantly up-regulated in the resistant group compared to the control and the susceptible groups. Those proteins are associated with immunostimulatory effects, signalling and binding cascade, metabolism, and maintaining tissue integrity and physiological condition. Besides, potential protein biomarkers related to the immune system were identified, which could be associated with the disease-resistant phenotype. These data provide insights into the underlying immune mechanism of hybrid groupers upon Vibrio sp. infection.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140206990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inactivated Aeromonas salmonicida impairs adaptive immunity in lumpfish (Cyclopterus lumpus) 灭活的沙门氏菌（Aeromonas salmonicida）会损害鳞鱼（Cyclopterus lumpus）的适应性免疫。

IF 2.5 3区农林科学

Journal of fish diseases Pub Date : 2024-03-24 DOI: 10.1111/jfd.13944

Setu Chakraborty, Hajarooba Gnanagobal, Ahmed Hossain, Trung Cao, Ignacio Vasquez, Danny Boyce, Javier Santander

{"title":"Inactivated Aeromonas salmonicida impairs adaptive immunity in lumpfish (Cyclopterus lumpus)","authors":"Setu Chakraborty, Hajarooba Gnanagobal, Ahmed Hossain, Trung Cao, Ignacio Vasquez, Danny Boyce, Javier Santander","doi":"10.1111/jfd.13944","DOIUrl":"10.1111/jfd.13944","url":null,"abstract":"Aeromonas salmonicida, a widely distributed aquatic pathogen causing furunculosis in fish, exhibits varied virulence, posing challenges in infectious disease and immunity studies, notably in vaccine efficacy assessment. Lumpfish (Cyclopterus lumpus) has become a valuable model for marine pathogenesis studies. This study evaluated several antigen preparations against A. salmonicida J223, a hypervirulent strain of teleost fish, including lumpfish. The potential immune protective effect of A. salmonicida bacterins in the presence and absence of the A-layer and extracellular products was tested in lumpfish. Also, we evaluated the impact of A. salmonicida outer membrane proteins (OMPs) and iron-regulated outer membrane proteins (IROMPs) on lumpfish immunity. The immunized lumpfish were intraperitoneally (i.p.) challenged with 104 A. salmonicida cells/dose at 8 weeks-post immunization (wpi). Immunized and non-immunized fish died within 2 weeks post-challenge. Our analyses showed that immunization with A. salmonicida J223 bacterins and antigen preparations did not increase IgM titres. In addition, adaptive immunity biomarker genes (e.g., igm, mhc-ii and cd4) were down-regulated. These findings suggest that A. salmonicida J223 antigen preparations hinder lumpfish immunity. Notably, many fish vaccines are bacterin-based, often lacking efficacy evaluation. This study offers crucial insights for finfish vaccine approval and regulations.","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfd.13944","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140206991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0